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Responses Of Artemisia Herba-Alba Asso. And Its Associated Species To Grazing Frequency (Midelt, Morocco), M Mejjati Alami, O Berkat, C Bensalem Jul 2024

Responses Of Artemisia Herba-Alba Asso. And Its Associated Species To Grazing Frequency (Midelt, Morocco), M Mejjati Alami, O Berkat, C Bensalem

IGC Proceedings (1977-2023)

The objective of this study (conducted in 1994) was to assess the effects of season and grazing frequency (control, continuous, intermittent during 2 days per week) on vegetation attributes, and animal feeding behavior. Mean standing crop, for which Artemisia herba alba contributed 72%, attained 533 for the control while it was maintained at 394 and 322 Kg DM/ha, for the continuous and the intermittent grazing, respectively. Mean productivity of Artemisia herba alba varied according to treatment and had values of 5.3, 3.0 and 2.5 Kg DM/ha/day, under the ungrazed control, the continuous and the intermittent grazing regimes, respectively. Percent utilization …


Grazing Management Of An Italian Ryegrass Sward With Dairy Sheep In The Mediterranean Environment, M Sitzia, P P. Roggero, N Fois, G Molle Jul 2024

Grazing Management Of An Italian Ryegrass Sward With Dairy Sheep In The Mediterranean Environment, M Sitzia, P P. Roggero, N Fois, G Molle

IGC Proceedings (1977-2023)

A grazing experiment was conducted in North-West Sardinia with the aim of testing the sward height as a mean to set up an efficient spring management of Italian ryegrass (Lolium multiflorum Lam.). Three fields were continuously grazed during spring 1992 by three groups of 12 dairy Sarda ewes, at three different sward heights (SH): 30 - 60 - 90 mm. The target sward height was obtained by continuously adjusting the field sizes. The results showed that the different grazing managements influenced the above ground biomass production and partitioning of the Italian ryegrass and the animal performances. During the vegetative …


Estimation Of Herbage Intake Of High Producing Milking Cows Under Intensive Grazing With A Supplementary Feeding Method In Northern Japan, K Ohiai, K Sudo, T Ikeda, T Honma Jul 2024

Estimation Of Herbage Intake Of High Producing Milking Cows Under Intensive Grazing With A Supplementary Feeding Method In Northern Japan, K Ohiai, K Sudo, T Ikeda, T Honma

IGC Proceedings (1977-2023)

The objective of this study was to estimate the herbage intake and to assess a supplementary feeding method for high producing milk cows under intensive grazing. Eight high producing milking cows were grazed rotationally on perennial ryegrass (Lolium perenne L) dominant mixed pasture and on meadow fescue (Festuca elatior L) dominant mixed pasture, changing rotation period and grazing area ccording to seasonal pasture growth rate. Pasture intakes were estimated to be about 2% of body weight by TDN requirements estimation of animals. A supplementary feeding method is examined to be proper for high producing milking cows under …


Palatability Of Grasses And Grazing On Hungarian Grassland, Z Barcsak, I Kertesz, L Szeman Jul 2024

Palatability Of Grasses And Grazing On Hungarian Grassland, Z Barcsak, I Kertesz, L Szeman

IGC Proceedings (1977-2023)

Grasslands provide the most basic and cheapest forage diet for cattle. The palatability studies were carried out on pure and mixed swards sown in 1990 on the GATE experimental farm. The studies involved the following 12 species: Lolium perenne, Trifolium repens, Festuca arundinacea, Festuca rubra, Poa pratensis, Festuca pratensis, Bromus inermis, Phalaris arundinacea, Lotus corniculatus, Agropyron crystatum, Dactylis glomerata and a mixed sward. During the study the morning and afternoon grazings were investigated with the involvement of 60 Limousin cattle. The following observations were made: 1. On the basis of our ethological observations regarding the bite rates the animals found …


Selective Sheep Grazing In An Heterogeneous Wheatgrass Dominated Pasture Related To Stocking Rate, G L. Siffredi, M A. Brizuela, M S. Cid, C Cangiano Jul 2024

Selective Sheep Grazing In An Heterogeneous Wheatgrass Dominated Pasture Related To Stocking Rate, G L. Siffredi, M A. Brizuela, M S. Cid, C Cangiano

IGC Proceedings (1977-2023)

It has been hypothesized that patch and community selection are driven by forage quality and/or quantity. We assess the relationship between vegetation characteristics and community and patch selection by sheep under continuous grazing at different stocking rates in an heterogeneous pasture. Our results suggest that when communities strongly differed in nutritive value sheep recurrently select the higher quality community in spite of its low biomass. However, sheep do not completely exclude the poorer quality community, generating a patchy vegetation pattern.


Grazing Management Of Elephant-Grass For Milk Production, F Deresz, L L. Matos Jul 2024

Grazing Management Of Elephant-Grass For Milk Production, F Deresz, L L. Matos

IGC Proceedings (1977-2023)

The objectives of these experiments were to study the effects of three resting periods (RP) of elephant-grass pasture on milk production and composition of Holstein-Zebu cows. These trials were carried out during two consecutive rainy seasons (December to May) using 24 cows in each year. They were randomly assigned by blocks according to milk yield and live weight (LW) to four treatments: 30- day RP with no concentrate (30N), 30-day RP with concentrate (30C), 36 and 45-day RP with C (36C and 45C) in the first year. In the second year the treatments were: 30C, 30N, 36N and 45N. Concentrate …


Comparison Of Yield, Forage Quality And Persistence Of 11 Grasses Grown With Irrigation In A Semi-Arid Climate, D E. Glover, G A. Kielly, R H. Cohen, P G. Jefferson Jul 2024

Comparison Of Yield, Forage Quality And Persistence Of 11 Grasses Grown With Irrigation In A Semi-Arid Climate, D E. Glover, G A. Kielly, R H. Cohen, P G. Jefferson

IGC Proceedings (1977-2023)

Nutritive quality of many grass species used for forage in the Northern Great Plains of Canada and the United States is not well documented, especially at different maturity stages. This study compared agronomic and forage quality characteristics of eleven grasses common to Saskatchewan. Botanical separation and quality analysis was undertaken at three growth stages. Meadow Brome (Bromus riparius), Russian Wildrye (Psathyrostachys juceus) and Altai Wildrye (Elymus angustus) were shown to have high leaf to stem ratios which corresponded with high protein levels. Separations showed persistence of Reed Canarygrass (Phalaris arudinaceus), Creeping Foxtail …


The Effect Of Grazing On Leaf Development In Eight Grass Species, H A. Lardner, S.B M. Wright, R H. Cohen Jul 2024

The Effect Of Grazing On Leaf Development In Eight Grass Species, H A. Lardner, S.B M. Wright, R H. Cohen

IGC Proceedings (1977-2023)

Grazing by herbivores affects grass species both morphologically and physiologically. A grazing study was conducted on an irrigated pasture near Outlook, Saskatchewan, Canada during the summers of 1991 and 1992 to determine morphological development of regrowth of reed canarygrass (Phalaris arundinacea L.), slender wheatgrass (Elymus trachycaulus (Link) Gould ex Shinners subsp. trachycaulus), intermediate wheatgrass (Thinopyrum intermedium (Host) Barkw. & D.R. Dewey subsp. intermedium), orchardgrass (Dactylis glomerata L.), meadow bromegrass (Bromus beibersteinii Roem. & Schult.), smooth bromegrass (Bromus inermis Leyss.), tall fescue (Festuca arundinacea (Schreb.) Wimm.) and timothy (Phleum pratense L.) in order to …


The Effect Of Slurry And Dirty Water Application To Swards On Grazing Behaviour In Dairy Cows, S Danby, J A. Laws, P D. Penning, B F. Pain, E Owen Jul 2024

The Effect Of Slurry And Dirty Water Application To Swards On Grazing Behaviour In Dairy Cows, S Danby, J A. Laws, P D. Penning, B F. Pain, E Owen

IGC Proceedings (1977-2023)

In preference tests using pairs of turves in boxes (treated and control), dairy cows were more averse to surface spread than shallow injected slurry and more averse to slurry than dirty water. Effects of slurry persisted for up to 6 weeks, with fewer bites and less herbage removed from treated swards compared to control swards. Aversion to slurry may be decreased by injection. Effects of dirty water persisted for only 14 days.


Applications Of Height-Based Grazing Management In Mixed Swards, A Singh, J E. Johnston, E A. Clark Jul 2024

Applications Of Height-Based Grazing Management In Mixed Swards, A Singh, J E. Johnston, E A. Clark

IGC Proceedings (1977-2023)

Grazing efficiency and livestock performance were compared on swards rotationally grazed down to either 50 or 100 mm for three years. With a fixed entry height of 200-250 mm, standing swards of mixed grasses and white clover (Trifolium repens L.) were stratified into 50 mm vertical layers both pre- and post-grazing. Through four or five cycles of grazing each year for three years, a fixed entry height range stabilized total standing herbage at 3250 to 3690 kg OM ha-1. Organic matter on-offer above 100 mm was 36, 45, and 43% of total standing herbage in the three years, compared …


Ingestive Behavior Of Fistulated And Intact Sheep Grazing Grass And Legumes Indoors And Outdoors, Y M. Gong, J Hodgson, M G. Lambert Jul 2024

Ingestive Behavior Of Fistulated And Intact Sheep Grazing Grass And Legumes Indoors And Outdoors, Y M. Gong, J Hodgson, M G. Lambert

IGC Proceedings (1977-2023)

Components of short-term ingestive behaviour were recorded for fistulated and intact sheep grazing turves in indoor crates, and for esophageal-fistulated sheep grazing swards in situ in outdoor cages. Perennial ryegrass, orchard grass and white clover at vegetative and reproductive growth stages were offered to the sheep. Bite weight, depth, area and volume did not differ between sheep grazing indoors and outdoors, nor between intact and fistulated sheep. Bite rate of fistulated sheep was slower indoors than outdoors (25.3 versus 32.1 bites/min), and intact sheep had a higher biting rate (36.7 versus 25.3 bites/min) than fistulated sheep. Bite weight was over-estimated …


Dairy Production From Pasture In Southern China, P.N P. Matthews, J Hodgson, A.C P. Chu, W L. Jiang, C B. Luo Jul 2024

Dairy Production From Pasture In Southern China, P.N P. Matthews, J Hodgson, A.C P. Chu, W L. Jiang, C B. Luo

IGC Proceedings (1977-2023)

This paper covers the development of dairy production systems as part of the UNDP funded Agro-grasslands Systems Development Project in Guizhou Province, China. The basic philosophy for the project was for local adaptation of New Zealand technology for developing dairy production systems based on pastures. The dairy production systems were established and the outputs of the project successfully achieved. Net incomes of smallholder farmers increased from a baseline of RMB $140 to RMB $1740 per annum over a three year period. Based on the experienced gained in setting up these systems local researchers, administrators, extension personnel and smallholders were able …


Trace Mineral Status Of Beef Cattle Grazing Semiarid Rangelands Of North Mexico, J R. Kawas, G T. Armienta, E Olivares, O Torress, J J. Kawas, F Garza Jul 2024

Trace Mineral Status Of Beef Cattle Grazing Semiarid Rangelands Of North Mexico, J R. Kawas, G T. Armienta, E Olivares, O Torress, J J. Kawas, F Garza

IGC Proceedings (1977-2023)

Trace mineral concentrations of soil, forage and serum of range cattle of the northeast Mexico state of Nuevo Leon were determined in both wet and dry seasons of 1992. For each season, collections were made at twenty-four ranches within eleven counties of three regions. A total of 220 soil, 680 forage and 220 blood samples were collected and analyzed. No differences (P<0.05) between regions were obtained for Zinc (28.0 to 34.9 ppm) or Copper (4.4 to 5.4 ppm). Molybdenum concentrations were greater in the northern (2.1 ppm) than in the central (1.6 ppm) or southern (1.5 ppm) regions. Considering the critical levels in forages for Zinc (30 ppm) and Copper (10 ppm) reported by the National Research Council (1984), Zinc concentrations may be marginally deficient, whereas Copper concentrations were deficient in all three regions, in both seasons, and in grasses and woody species.


Forage-Based Seasonal Dairying In South Louisiana, H A. Deramus, L Labbe, K Ingawa, W A. Atkinson, M J. Simon, J D. Roussel Jul 2024

Forage-Based Seasonal Dairying In South Louisiana, H A. Deramus, L Labbe, K Ingawa, W A. Atkinson, M J. Simon, J D. Roussel

IGC Proceedings (1977-2023)

The effects on the net profit and total milk production were compared using two levels of grain supplementation and forage-only feeding in a program of seasonal dairying in South Louisiana using management intensive grazing. Treatments were 8 kg grain, 4 kg grain, and 0 grain supplementation. Annual ryegrass (Lolium multiflorium) and clovers (Trifolium incarnatum, and T. Alexandrinum) were grazed intensively with 51 multiparous Jersey and Holstein cows in mid-lactation in 1993-1995. Milk production, milk fat, milk protein, and blood urea nitrogen were measured. Milk and protein models were all highly significant for all classes. Daily milk …


Influence Of Sheep And Goats On The Natural Succession, H D. Matthes, S Demise, M Schubert, H Mohring Jul 2024

Influence Of Sheep And Goats On The Natural Succession, H D. Matthes, S Demise, M Schubert, H Mohring

IGC Proceedings (1977-2023)

A study was conducted to examine the effect of extensively-grazed pasture on the botanical composition. The trails were carried out in the year 1992 to 1995. For the study different breeds of sheep with the stocking rate of 30 sheep with their lambs / 2.6 ha were used. For the mixed-grazing a ratio of 20 sheep and 10 goats with their lambs / 2.6 ha were used. It is not possible to control all problem species with the help of sheepgrazing only. In contrast to that, it was concluded that goats grazed off all problem species quite effectively. Botanical composition …


Influence Of Different Breeds Of Pigs To The Natural Succession, H D. Matthes, A.C D. Micklich, G Dietl, H Mohring, W Jentsch Jul 2024

Influence Of Different Breeds Of Pigs To The Natural Succession, H D. Matthes, A.C D. Micklich, G Dietl, H Mohring, W Jentsch

IGC Proceedings (1977-2023)

The aim of this study was to find out the effect of pigs on the natural succession. We used 4 different breeds of pigs for our investigations. Because of the grazing behaviour and their rooting activity the total number of plants increased up to 30%. But the vegetation quickly returned to conditions prior to rooting, if the rooting activity fails to happen. Rooting activity of pigs on floodplain soil and following grazing in the next spring after rooting permits seeds of different species of plants to germinate and develop in the plant free areas. These plants often have little chance …


Nutrition And Dietetics Educators’ Experiences Using Systems Thinking In Teaching, Erin E. Bergquist, Lyndi Buckingham-Schutt, Scott Smalley, Christina G. Campbell, Awoke Dollisso, Shuyang Qu Jul 2024

Nutrition And Dietetics Educators’ Experiences Using Systems Thinking In Teaching, Erin E. Bergquist, Lyndi Buckingham-Schutt, Scott Smalley, Christina G. Campbell, Awoke Dollisso, Shuyang Qu

Journal of Dietetic Education

Objective: Describe educator experiences teaching systems thinking in nutrition and dietetics education. Design: Qualitative, semi-structured, online interviews. Setting: Educators teaching in accredited nutrition and dietetics programs. Participants: Purposive sample of thirteen Registered Dietitian Nutritionists (92% female, 77% with > 6 years teaching experience) from each U.S. census region and accredited program type. Phenomenon of Interest: Educators described experiences teaching systems thinking in accredited programs, including benefits. Analysis: Transcribed interviews were analyzed using qualitative grounded theory iterative coding. Excel was used for data organization and thematic coding analysis. Results: Educators described four themes when teaching systems thinking: (1) a holistic approach to …


Transnational Entrepreneurship And The Role Of Cultural Distance: Case Study Research On African Transnational Entrepreneurs, Jörg Freiling Jul 2024

Transnational Entrepreneurship And The Role Of Cultural Distance: Case Study Research On African Transnational Entrepreneurs, Jörg Freiling

European Management Studies

Purpose: While transnational entrepreneurship research considers its transcultural dimension, the role of vast cultural distance is largely ignored. Thus, this paper focuses on the following research question: how do the (cross-)cultural peculiarities of transnational entrepreneurship – in the light of cultural distance – influence the business development of transnational entrepreneurs? Design/methodology/approach: The paper employs a qualitative empirical design that rests on case study research framed by the ‘Gioia’ method. Findings: The paper identifies specific cultural challenges, cross-cultural drivers and general assets impacting transnational business developments in the case of vast cultural distance as aggregate dimensions. Implications: Transnational entrepreneurship in cultural …


The Cålåpitå Past The “Dull Edge” Of Extinction: A Shaggy Dog Story Of Repatriation And Refusal In Bali, Tyler Yamin Jul 2024

The Cålåpitå Past The “Dull Edge” Of Extinction: A Shaggy Dog Story Of Repatriation And Refusal In Bali, Tyler Yamin

Faculty Contributions to Books

No abstract provided.


Seth Lins V. Pa Society For The Prevention Of Cruelty To Animal Jul 2024

Seth Lins V. Pa Society For The Prevention Of Cruelty To Animal

2024 Decisions

USDC for the Eastern District of Pennsylvania


Lindsay Ortega Plaintiff-Appellee, V. Cynthia Jones Defendant-Appellant : Brief Of Appellant, Utah Court Of Appeals Jul 2024

Lindsay Ortega Plaintiff-Appellee, V. Cynthia Jones Defendant-Appellant : Brief Of Appellant, Utah Court Of Appeals

Utah Court of Appeals Briefs (2020 –)

Appeal from [Modified by the Court] Final Order and Judgment in Favor or Plaintiff Lindsay Ortega, Fourth Judicial District Court, Wasatch County, State of Utah, District Court No. 160500012, Honorable Jennifer A. [Brown] Mabey presiding Appellant’s Opening Brief Patricia Geary Glenn (#5280)

ATTORNEY AT LAW, P.C. Attorney for Defendant

/Appellant ii TABLE OF CONTENTS IDENTIFICATION OF PARTIES ........................................................................iv INTRODUCTION .................................................................................................... 1 STATEMENT OF THE ISSUES ............................................................................ 5 1. Whether the district court erred in reinstating its initial 2018 Final Order and Judgment after excluding the evidence which formed the basis of its decision as improperly proffered by Plaintiff under Rule …


Ion-Mediated Structural Discontinuities In Phospholipid Vesicles, Judith U. De Mel, Stefanie Klisch, Sudipta Gupta, Gerald J. Schneider Jul 2024

Ion-Mediated Structural Discontinuities In Phospholipid Vesicles, Judith U. De Mel, Stefanie Klisch, Sudipta Gupta, Gerald J. Schneider

Faculty Publications

Despite intense research, methods for controlling soft matter’s spontaneous self-assembly into well-defined layers remain a significant challenge. We observed ion-induced structural discontinuities of phospholipid vesicles that can be exploited for controlled self-assembly of soft materials, using DOPC and NaCl as a model system. The observations were made for the 0.25 wt % lipid concentration. We used dynamic light scattering, zeta-potential measurement, cryo-electron microscopy, small-angle X-ray, and small-angle neutron scattering to understand the reason for the discontinuities. For salt concentrations below 8 mM, we observed a decrease in the liposome diameter with increased NaCl concentration. Above 8 mM, we measured a …


Susceptibility Of Spodoptera Frugiperda And Helicoverpa Zea Populations In The Southern Region Of The United States To Common Chemical Insecticides, Bhavana Patla Jul 2024

Susceptibility Of Spodoptera Frugiperda And Helicoverpa Zea Populations In The Southern Region Of The United States To Common Chemical Insecticides, Bhavana Patla

LSU Master's Theses

Abstract

The fall armyworm, Spodoptera frugiperda and corn earworm/bollworm, Helicoverpa zea, are two polyphagous pests prevalent in the U.S. In recent years, there were increased infestations of the two pests in the U.S., especially in its southern region. Information on the susceptibility/resistance status of the two pests in the region to chemical insecticides, especially to some new chemistries, is very limited or has not been updated for many years. In this study, susceptibility of 17 S. frugiperda and six H. zea field collected populations from the southern U.S. during 2021-23 to common insecticides was investigated in the laboratory. Dose-responses with …


Experiment With The Scratch Application, Orit D. Gruber, Daniel Agman Jul 2024

Experiment With The Scratch Application, Orit D. Gruber, Daniel Agman

Open Educational Resources

SCRATCH is a free and fun online application for exploring and creating animated programs, games, interactive stories, and projects.

Refer to the following link for an overview of Scratch: https://scratch.mit.edu/

You will be working with four(4) different starter programs; each starter program should be selected from a different category.


Multi-Antigen Intranasal Vaccine Protects Against Challenge With Sarbecoviruses And Prevents Transmission In Hamsters, Ankita Leekha, Arash Saeedi, K M Samiur Rahman Sefat, Monish Kumar, Melisa Martinez-Paniagua, Adrian Damian, Rohan Kulkarni, Kate Reichel, Ali Rezvan, Shalaleh Masoumi, Xinli Liu, Laurence J N Cooper, Manu Sebastian, Courtney M Sands, Vallabh E Das, Nimesh B Patel, Brett Hurst, Navin Varadarajan Jul 2024

Multi-Antigen Intranasal Vaccine Protects Against Challenge With Sarbecoviruses And Prevents Transmission In Hamsters, Ankita Leekha, Arash Saeedi, K M Samiur Rahman Sefat, Monish Kumar, Melisa Martinez-Paniagua, Adrian Damian, Rohan Kulkarni, Kate Reichel, Ali Rezvan, Shalaleh Masoumi, Xinli Liu, Laurence J N Cooper, Manu Sebastian, Courtney M Sands, Vallabh E Das, Nimesh B Patel, Brett Hurst, Navin Varadarajan

Faculty, Staff and Student Publications

Immunization programs against SARS-CoV-2 with commercial intramuscular vaccines prevent disease but are less efficient in preventing infections. Mucosal vaccines can provide improved protection against transmission, ideally for different variants of concern (VOCs) and related sarbecoviruses. Here, we report a multi-antigen, intranasal vaccine, NanoSTING-SN (NanoSTING-Spike-Nucleocapsid), eliminates virus replication in both the lungs and the nostrils upon challenge with the pathogenic SARS-CoV-2 Delta VOC. We further demonstrate that NanoSTING-SN prevents transmission of the SARS-CoV-2 Omicron VOC (BA.5) to vaccine-naïve hamsters. To evaluate protection against other sarbecoviruses, we immunized mice with NanoSTING-SN. We showed that immunization affords protection against SARS-CoV, leading to protection …


Cell-Specific Regulation Of The Circadian Clock By Bmal1 In The Paraventricular Nucleus: Implications For Regulation Of Systemic Biological Rhythms, Rachel Van Drunen, Yulin Dai, Haichao Wei, Baharan Fekry, Sina Noori, Samay Shivshankar, Rafael Bravo, Zhongming Zhao, Seung-Hee Yoo, Nicholas Justice, Jia Qian Wu, Qingchun Tong, Kristin Eckel-Mahan Jul 2024

Cell-Specific Regulation Of The Circadian Clock By Bmal1 In The Paraventricular Nucleus: Implications For Regulation Of Systemic Biological Rhythms, Rachel Van Drunen, Yulin Dai, Haichao Wei, Baharan Fekry, Sina Noori, Samay Shivshankar, Rafael Bravo, Zhongming Zhao, Seung-Hee Yoo, Nicholas Justice, Jia Qian Wu, Qingchun Tong, Kristin Eckel-Mahan

Faculty, Staff and Student Publications

Circadian rhythms are internal biological rhythms driving temporal tissue-specific, metabolic programs. Loss of the circadian transcription factor BMAL1 in the paraventricular nucleus (PVN) of the hypothalamus reveals its importance in metabolic rhythms, but its functions in individual PVN cells are poorly understood. Here, loss of BMAL1 in the PVN results in arrhythmicity of processes controlling energy balance and alters peripheral diurnal gene expression. BMAL1 chromatin immunoprecipitation sequencing (ChIP-seq) and single-nucleus RNA sequencing (snRNA-seq) reveal its temporal regulation of target genes, including oxytocin (OXT), and restoring circulating OXT peaks in BMAL1-PVN knockout (KO) mice rescues absent activity rhythms. While glutamatergic neurons …


Nine Students Land Gilman Scholarships To Study Internationally, Keith Testa Jul 2024

Nine Students Land Gilman Scholarships To Study Internationally, Keith Testa

UNH Today Archive

No abstract provided.


Antibody-Activation Of Connexin Hemichannels In Bone Osteocytes With Atp Release Suppresses Breast Cancer And Osteosarcoma Malignancy, Manuel A Riquelme, Xuewei Wang, Francisca M Acosta, Jingruo Zhang, Jeffery Chavez, Sumin Gu, Peng Zhao, Wei Xiong, Ningyan Zhang, Guo Li, Saranya Srinivasan, Chaoyu Ma, Manjeet K Rao, Lu-Zhe Sun, Nu Zhang, Zhiqiang An, Jean X Jiang Jul 2024

Antibody-Activation Of Connexin Hemichannels In Bone Osteocytes With Atp Release Suppresses Breast Cancer And Osteosarcoma Malignancy, Manuel A Riquelme, Xuewei Wang, Francisca M Acosta, Jingruo Zhang, Jeffery Chavez, Sumin Gu, Peng Zhao, Wei Xiong, Ningyan Zhang, Guo Li, Saranya Srinivasan, Chaoyu Ma, Manjeet K Rao, Lu-Zhe Sun, Nu Zhang, Zhiqiang An, Jean X Jiang

The Brown Foundation: Institute of Molecular Medicine

Bone tissue represents the most frequent site of cancer metastasis. We developed a hemichannel-activating antibody, Cx43-M2. Cx43-M2, directly targeting osteocytes in situ, activates osteocytic hemichannels and elevates extracellular ATP, thereby inhibiting the growth and migration of cultured breast and osteosarcoma cancer cells. Cx43-M2 significantly decreases breast cancer metastasis, osteosarcoma growth, and osteolytic activity, while improving survival rates in mice. The antibody's inhibition of breast cancer and osteosarcoma is dose dependent in both mouse and human cancer metastatic models. Furthermore, Cx43-M2 enhances anti-tumor immunity by increasing the population and activation of tumor-infiltrating immune-promoting effector T lymphocytes, while reducing immune-suppressive regulatory T …


Endoplasmic Reticulum Morphology Regulation By Rtn4 Modulates Neuronal Regeneration By Curbing Luminal Transport., Laura M. Westrate, Pierre Parutto, Cecile C. Crapart, Elena F. Koslover, Edward Avezov, Tasuku Konno Jul 2024

Endoplasmic Reticulum Morphology Regulation By Rtn4 Modulates Neuronal Regeneration By Curbing Luminal Transport., Laura M. Westrate, Pierre Parutto, Cecile C. Crapart, Elena F. Koslover, Edward Avezov, Tasuku Konno

University Faculty Publications and Creative Works

Cell functions rely on intracellular transport systems distributing bioactive molecules with high spatiotemporal accuracy. The endoplasmic reticulum (ER) tubular network constitutes a system for delivering luminal solutes, including Ca2+, across the cell periphery. How the ER structure enables this nanofluidic transport system is unclear. Here, we show that ER membrane-localized reticulon 4 (RTN4/Nogo) is sufficient to impose neurite outgrowth inhibition in human cortical neurons while acting as an ER morphoregulator. Improving ER transport visualization methodologies combined with optogenetic Ca2+ dynamics imaging and in silico modeling, we observed that ER luminal transport is modulated by ER tubule narrowing and dilation, proportional …


Dna Fork Remodeling Proteins, Zranb3 And Smarcal1, Are Uniquely Essential For Aging Hematopoiesis, Saul Kushinsky, Matthew V. Puccetti, Clare M. Adams, Irina Shkundina, Nikkole James, Brittany M. Mahon, Peter Michener, Christine M. Eischen Jul 2024

Dna Fork Remodeling Proteins, Zranb3 And Smarcal1, Are Uniquely Essential For Aging Hematopoiesis, Saul Kushinsky, Matthew V. Puccetti, Clare M. Adams, Irina Shkundina, Nikkole James, Brittany M. Mahon, Peter Michener, Christine M. Eischen

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Over a lifetime, hematopoietic stem and progenitor cells (HSPCs) are forced to repeatedly proliferate to maintain hematopoiesis, increasing their susceptibility to DNA damaging replication stress. However, the proteins that mitigate this stress, protect HSPC replication, and prevent aging-driven dysregulation are unknown. We report two evolutionarily conserved, ubiquitously expressed chromatin remodeling enzymes with similar DNA replication fork reversal biochemical functions, Zranb3 and Smarcal1, have surprisingly specialized roles in distinct HSPC populations. While both proteins actively mitigate replication stress and prevent DNA damage and breaks during lifelong hematopoiesis, the loss of either resulted in distinct biochemical and biological consequences. Notably, defective long-term …