Digital Commons Network^™

Recent Progress In Modeling And Treating Diabetes Using Stem Cell-Derived Islets, Marlie M Maestas, Maggie H Bui, Jeffrey R Millman

2020-Current year OA Pubs

Stem cell-derived islets (SC-islets) offer the potential to be an unlimited source of cells for disease modeling and the treatment of diabetes. SC-islets can be genetically modified, treated with chemical compounds, or differentiated from patient derived stem cells to model diabetes. These models provide insights into disease pathogenesis and vulnerabilities that may be targeted to provide treatment. SC-islets themselves are also being investigated as a cell therapy for diabetes. However, the transplantation process is imperfect; side effects from immunosuppressant use have reduced SC-islet therapeutic potential. Alternative methods to this include encapsulation, use of immunomodulating molecules, and genetic modification of SC-islets. …

Molecular Characterization Of Sterol C4-Methyl Oxidase In Leishmania Major, Yu Ning, Somrita Basu, Fong-Fu Hsu, Mei Feng, Michael Zhuo Wang, Kai Zhang

2020-Current year OA Pubs

Sterol biosynthesis requires the oxidative removal of two methyl groups from the C-4 position by sterol C-4-demethylase and one methyl group from the C-14 position by sterol C-14-demethylase. In

A Simple And Fast Optical Clearing Method For Whole-Mount Fluorescence In Situ Hybridization (Fish) Imaging, Junwoo Suh, Yehe Liu, Jordan Smith, Michiko Watanabe, Andrew M. Rollins, Michael W. Jenkins

Faculty Scholarship

We report a single-step optical clearing method that is compatible with RNA fluorescence in situ hybridization (FISH) imaging. We previously demonstrated microscopy imaging with immunohistochemistry and genetic reporters using a technique called lipid-preserving refractive index matching for prolonged imaging depth (LIMPID). Our protocol reliably produces high-resolution three-dimensional (3D) images with minimal aberrations using high magnification objectives, captures large field-of-view images of whole-mount tissues, and supports co-labeling with antibody and FISH probes. We also custom-designed FISH probes for quail embryos, demonstrating the ease of fabricating probes for use with less common animal models. Furthermore, we show high-quality 3D images using a …

Dubai Urban Planning 2040: Resource Requirements In The Urban District 5, Bakhit Alfalasi

Theses

The availability of water, food, energy and air quality resources is compromised by prevailing global issues like environmental stress, climate change, and population growth. This research study details the different requirements for Dubai urban District 5 based on the tenets laid out in the Urban Planning 2040 initiative. The literature review section looks into existing literature on the present state of affairs in the UAE as regards availability of water, food, and energy resources in the country and implications of their production processes on air quality. The Study will employ an exploratory research design, where a systematic review of literature …

Montana Kaimin, October 10, 2024, Students Of The University Of Montana, Missoula

Montana Kaimin, 1898-present

Student newspaper of the University of Montana, Missoula.

Quaker Campus, October 10, 2024 (Vol. 23, Issue 4), Whittier College

Quaker Campus

No abstract provided.

Whole Genome Analyses Of Introgression In British And Irish Apis Mellifera Mellifera, Victoria G. Buswell, J. Vanessa Huml, Jonathan S. Ellis, Andrew Brown, Mairi E. Knight

School of Biological and Marine Sciences

The Western honey bee, Apis mellifera (A. mellifera) Linnaeus, is one of the world’s most economically important pollinators. Conserving genetic resources in this species is therefore of significant value. Despite this, little attention has been paid to the potential erosion of locally adapted genetic variants by transhumance of particular subspecies favoured by beekeepers. In particular, introgression and hybridisation by repeated backcrossing can disrupt naturally-selected allele combinations and therefore erode local adaptation. Here we use whole genome sequencing (WGS) to assess levels of introgression in the native subspecies Apis mellifera mellifera (A. m. mellifera) across a wide geographical scale in Britain …

Systematic Perturbations Of Setd2, Nsd1, Nsd2, Nsd3, And Ash1l Reveal Their Distinct Contributions To H3k36 Methylation, Gerry A Shipman, Reinnier Padilla, Cynthia Horth, Bo Hu, Eric Bareke, Francisca N Vitorino, Joanna M Gongora, Benjamin A Garcia, Chao Lu, Jacek Majewski

2020-Current year OA Pubs

BACKGROUND: Methylation of histone 3 lysine 36 (H3K36me) has emerged as an essential epigenetic component for the faithful regulation of gene expression. Despite its importance in development and disease, how the molecular agents collectively shape the H3K36me landscape is unclear.

RESULTS: We use mouse mesenchymal stem cells to perturb the H3K36me methyltransferases (K36MTs) and infer the activities of the five most prominent enzymes: SETD2, NSD1, NSD2, NSD3, and ASH1L. We find that H3K36me2 is the most abundant of the three methylation states and is predominantly deposited at intergenic regions by NSD1, and partly by NSD2. In contrast, H3K36me1/3 are most …

Rapid Evolution Of Mitochondrion-Related Genes In Haplodiploid Arthropods, Yiyuan Li, Gregg W C Thomas, Stephen Richards, Robert M Waterhouse, Xin Zhou, Michael E Pfrender

Faculty, Staff and Students Publications

Background: Mitochondrial genes and nuclear genes cooperate closely to maintain the functions of mitochondria, especially in the oxidative phosphorylation (OXPHOS) pathway. However, mitochondrial genes among arthropod lineages have dramatic evolutionary rate differences. Haplodiploid arthropods often show fast-evolving mitochondrial genes. One hypothesis predicts that the small effective population size of haplodiploid species could enhance the effect of genetic drift leading to higher substitution rates in mitochondrial and nuclear genes. Alternatively, positive selection or compensatory changes in nuclear OXPHOS genes could lead to the fast-evolving mitochondrial genes. However, due to the limited number of arthropod genomes, the rates of evolution for nuclear …

Sestrin 2 As A New Biomarker In Obesity And Diabetes Mellitus: A New Key Player In The Metabolic Regulation, Shashank M Patil, Jayanthi M K, Ramith Ramu

International Journal of Health and Allied Sciences

The global health crisis of diabetes linked to obesity necessitates innovative biomarkers for early diagnosis and targeted treatment. This study explores the pivotal role of sestrins, evolutionarily conserved proteins, in the intricate interplay between obesity and diabetes. Highlighting their significance as regulators of metabolic balance, stress response, and cellular homeostasis, particularly sestrin 2 (SESN2), the study delves into their impact on oxidative stress, insulin sensitivity, and inflammatory responses. Recent studies have highlighted the dysregulation of sestrins in obesity and diabetes mellitus, suggesting their potential as diagnostic markers and therapeutic targets. Examining the molecular processes involving AMP-activated protein kinase (AMPK) and …

Curating And Validating Universally Applicable Primers For Efficient Its2-Based Dna Barcoding Across Plant Taxa, Ajay R Bhat, Subrahmanya Hegde, Supriya S Kammar, Mb Muthamma, Gaurav Mudgal, Prashanth Babu H, Rajulu Charukesi, Thotegowdanapalya C Mohan

International Journal of Health and Allied Sciences

Abstract: Rapid and accurate identification of plant species increasingly seeks to employ molecular techniques. The ITS2 region is highly regarded in DNA barcoding due to its short length and ease of sequencing, making it an ideal candidate for species identification

Epitope Identification Of Rabies Virus Nucleoprotein Using Immunoinformatics Approach, Kavitha G. Manjunatha, Chandrahasa Chethana, Akhila D. Sannejal, Mohammad Shueb, Devananda Devegowda, Shashanka K. Prasad, Deepak S. Pinto, Shrikrishna Isloor

International Journal of Health and Allied Sciences

Abstract

Background

Rabies is a deadly and preventable disease. The nucleoprotein of rabies virus has been found to have group-specific antigenic determinants. The rabies virus nucleoprotein can shield dogs and mice from the lethal infection. Early diagnosis of rabies is crucial for the prevention of rabies.

Methods

In this study, B-cell epitopes of the nucleoprotein gene of the rabies virus were identified, and the characteristics of the epitopes were analyzed using various bioinformatics tools, such as the immune epitope database's Bepipred Major Histocompatibility Complex II (IEDB MHC II) prediction tool, NetCTL 1.2, Vaxijen v20, AllerTOP v2.0 server.

Results

Fourteen epitopes …

Harnessing Nature's Power Of Azolla Pinnata Superfood With Biofertilizers For A Sustainable Future, Khalander Bibi, Sushma Shree Krishnappa, Charan Kumar Kachintaya, Shankramma Kalikeri, Lingaraju Honnuru Gurusiddappa

International Journal of Health and Allied Sciences

Azolla pinnata, a rapidly growing floating aquatic fern, flourishes in freshwater bodies throughout India. It forms clusters in marshes, drains, and rice fields, doubling its biomass every 1.9 days. Azolla, known for its versatility, is used in food, medicinal, water purification, biofertilizers, and livestock feed. Its use in animal diets improves live weight, protein efficiency, and feed conversion rates. Azolla has pharmaceutical benefits in addition to agricultural, including antioxidant and immunological stimulation, as well as hepatoprotective properties. It also plays an important part in phytoremediation and bioremediation. Azolla's potential extends to biofuel production, making it a promising feedstock for sustainable …

Vol. 32, No. 4-8 - Whole No. 173, David Bratman

Mythprint

Mythprint is the monthly bulletin of the Mythopoeic Society, a nonprofit educational organization devoted to the study, discussion, and enjoyment of myth and fantasy literature, especially the works of J.R.R. Tolkien, C.S. Lewis, and Charles Williams. To promote these interests, the Society publishes three magazines, maintains a World Wide Web site, and sponsors the annual Mythopoeic Conference and awards for fiction and scholarship, as well as local and written discussion groups.

Vol. 31, No. 9 - Whole No. 168, David Bratman

Mythprint

Mythprint is the monthly bulletin of the Mythopoeic Society, a nonprofit educational organization devoted to the study, discussion, and enjoyment of myth and fantasy literature, especially the works of J.R.R. Tolkien, C.S. Lewis, and Charles Williams. To promote these interests, the Society publishes three magazines, maintains a World Wide Web site, and sponsors the annual Mythopoeic Conference and awards for fiction and scholarship, as well as local and written discussion groups.

Vol. 31, No. 8 - Whole No. 167, David Bratman

Mythprint

Mythprint is the monthly bulletin of the Mythopoeic Society, a nonprofit educational organization devoted to the study, discussion, and enjoyment of myth and fantasy literature, especially the works of J.R.R. Tolkien, C.S. Lewis, and Charles Williams. To promote these interests, the Society publishes three magazines, maintains a World Wide Web site, and sponsors the annual Mythopoeic Conference and awards for fiction and scholarship, as well as local and written discussion groups.

Vol. 31, No. 7 - Whole No. 166, David Bratman

Mythprint

Mythprint is the monthly bulletin of the Mythopoeic Society, a nonprofit educational organization devoted to the study, discussion, and enjoyment of myth and fantasy literature, especially the works of J.R.R. Tolkien, C.S. Lewis, and Charles Williams. To promote these interests, the Society publishes three magazines, maintains a World Wide Web site, and sponsors the annual Mythopoeic Conference and awards for fiction and scholarship, as well as local and written discussion groups.

Vol. 31, No. 5 - Whole No. 164, David Bratman

Mythprint

Mythprint is the monthly bulletin of the Mythopoeic Society, a nonprofit educational organization devoted to the study, discussion, and enjoyment of myth and fantasy literature, especially the works of J.R.R. Tolkien, C.S. Lewis, and Charles Williams. To promote these interests, the Society publishes three magazines, maintains a World Wide Web site, and sponsors the annual Mythopoeic Conference and awards for fiction and scholarship, as well as local and written discussion groups.

Vol. 31, No. 3 - Whole No. 162, David Bratman

Mythprint

Mythprint is the monthly bulletin of the Mythopoeic Society, a nonprofit educational organization devoted to the study, discussion, and enjoyment of myth and fantasy literature, especially the works of J.R.R. Tolkien, C.S. Lewis, and Charles Williams. To promote these interests, the Society publishes three magazines, maintains a World Wide Web site, and sponsors the annual Mythopoeic Conference and awards for fiction and scholarship, as well as local and written discussion groups.

Vol. 31, No. 2 - Whole No. 161, David Bratman

Mythprint

Mythprint is the monthly bulletin of the Mythopoeic Society, a nonprofit educational organization devoted to the study, discussion, and enjoyment of myth and fantasy literature, especially the works of J.R.R. Tolkien, C.S. Lewis, and Charles Williams. To promote these interests, the Society publishes three magazines, maintains a World Wide Web site, and sponsors the annual Mythopoeic Conference and awards for fiction and scholarship, as well as local and written discussion groups.

Ligand Directed Signalling In Therapeutics, Julia Shield

Discussions

No abstract provided.

[Discussions] Vol. 16 Iss. 1

Discussions

No abstract provided.

Disemboweling Binaries: How Medical Texts And Gothic Fiction Propagate Deviance Through The Damaged Brain, Hailey Q. Barab

Discussions

No abstract provided.

The Problem With Direct Collateral Review, Jaden M. Lessnick

Pepperdine Law Review

Federal habeas review of state convictions is sharply circumscribed for a reason: Granting the writ of habeas corpus disrupts the federalism and finality interests that lie at the heart of state sovereignty over criminal law. Both the Antiterrorism and Effective Death Penalty Act (AEDPA) and the Supreme Court’s equitable bars to relief reflect the structural dangers inherent in collateral review of state convictions. Given the increasing unavailability of federal habeas relief, state prisoners have turned to another vehicle for collateral federal review, one that bypasses AEDPA’s demanding standard: direct review of state post-conviction proceedings. And regrettably, the Court has entertained …

Index To Volume 69

Merrill-Palmer Quarterly: A Peer Relations Journal

Index to Volume 69

Spartan Daily, October 9, 2024, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2024

Volume 163, Issue 20

Association Between Psychopharmacotherapy And Postpartum Hemorrhage, Frank Ian Jackson, Insaf Kouba, Natalie Meirowitz, Nathan A. Keller, Luis A. Bracero, Matthew J. Blitz

Specialty Medicine Faculty Publications

Background: Prior studies evaluating the relationship between psychopharmacotherapy (PPT), and postpartum hemorrhage (PPH) have yielded inconsistent findings. Clarifying this potential relationship is important for effective counseling and risk stratification.
Objectives: Our primary objective was to evaluate the association between prenatal exposure to PPT (any drug class) and the occurrence of PPH requiring transfusion of packed red blood cells (PPH+pRBC) after systematically adjusting for known hemorrhage risk factors at the time of admission for delivery. Secondary objectives were to evaluate the association between individual PPT drug classes and PPH+pRBC, and the association between treatment intensity of mental health condition and PPH+pRBC. …

No. 06: Enhancing Rural-Urban Linkages Through Food Remittances, Elizabeth Opiyo Onyango, Jonathan Crush, Samuel Owuor

Migration and Food Security (MiFOOD) Network

In many rapidly urbanizing African countries, food remittances from rural to urban areas play a vital role in maintaining rural-urban linkages. In Nairobi, Kenya, half of all households, regardless of migration status, receive food remittances from rural areas. Contrary to conventional beliefs, these food transfers are not solely linked to migration or poverty. Instead, they appear to be a long-standing, integral part of urban household resilience, especially for better-off households. As urbanization intensifies, policymakers should recognize the role of food remittances in ensuring food security for both migrants and non-migrant households. This brief explores key challenges such as economic inequality, …

October 9, 2024 (Vol. 109, No. 7), Daily Eastern News

Daily Eastern News: 2024-2025

No abstract provided.

Oregon State Rank Assessment For Catlow Tui Chub (Siphateles Bicolor Ssp. 2), Jesse Laney

Institute for Natural Resources Publications

Oregon state conservation status assessment for Catlow tui chub (Siphateles bicolor ssp. 2) using NatureServe methodology, 2024.