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Antimetastatic Effect Of Nanodiamond-Conjugated Quercetin Against Colon Cancer: An In Vivo Study, Firli Rahmah Primula Dewi, Sephia Tiara Marviella, Sri Puji Astuti Wahyuningsih, A'Liyatur Rosyidah, Vuanghao Lim, Lionel Lian Aun In, Amy Yi Hsan Saik, Bimaji Ariyogo, Mee Lee Looi

Turkish Journal of Biology

Background/aim: Quercetin (Q) is a compound that can inhibit the growth of cancer cells in the colon; however, to do so, a high dose is needed, requiring a drug delivery system to target cancer endothelial cells directly. This study investigates the potency of nanodiamond-conjugated quercetin (NDQ) as an anticancer drug against colon cancer in Rattus norvegicus induced by N-methyl N-Nitrosourea (MNU). Materials and methods: This study is experimental-based and was designed using a six-group treatment method, namely normal control (KN: not treated by MNU, nanodiamond (ND), or Q); negative control (K–: treated by MNU); positive control (K+: treated by MNU …

Delivery Of Bikdd Proapoptotic Gene In Peptide-18 Targeted Poly(2-Oxazoline)-Dope Nanoliposomes For Breast Cancer Models, Zeynep Büşra Bolat, Ayça Ece Nezi̇r, Ongun Mehmet Saka, Itir Ebru Zemheri̇, Sevgi̇ Gülyüz, Umut Uğur Özköse, Özgür Yilmaz, Asuman Bozkir, Di̇lek Telci̇, Fi̇kretti̇n Şahi̇n

Turkish Journal of Biology

Breast cancer is the most common cancer type and the major cause of death in females worldwide. For effective breast cancer treatment, systems with promising delivery of anticancer agents is an important strategy. Peptide 18 (P18), a tumor homing peptide, shows high binding affinity towards breast cancer cells. Nanoliposomes are known to have enhanced accumulation ability in tumors with longer systemic circulation. Poly (2-ethyl-2-oxazoline) (PEtOx) polymers conjugated with DOPE were used to prepare PEtOx-DOPE nanoliposomes. BikDD is a mutant form of Bik gene, a member of the BH3-only proapoptotic genes, mimics the constitutively phosphorylated form of the gene. To the …

Pterostilbene Suppresses Head And Neck Cancer Cell Proliferation Via Induction Of Apoptosis, Tali̇h Özdaş, Si̇bel Özdaş, İpek Canatar, Erdem Kaypak

Turkish Journal of Biology

Background/aim: Head and neck cancer (HNC) is one of the most prevalent causes of death worldwide, and the discovery of anticancer agents for its prevention is very important. Pterostilbene (PS) is a trans-stilbene reported to be beneficial in managing various cancers. The objective of the study was to evaluate the cytotoxic, anti-proliferative, pro-apoptotic and anti-migrative effect of PS on HEp-2, SCC-90, SCC-9, FaDu and Detroit-551 cell lines. Materials and Methods: MTT and live/dead assays were employed to assess cell-viability, while cell migration test was performed to evaluate wound healing capacity. The mRNA, protein and intracellular expression level of CASP-3, BAX …

Preparation Of Wound Dressings Using Levan From Lactobacillus Gasseri And Polyvinyl Alcohol Polymer, Jehan Abdul Sattar Salman, Shaimaa Ms Zainulabdeen

Muthanna Medical Journal

Levan had been extracted, purified, and characterized from Lactobacillus gasseri. Levan characterization had been carried out using "thin-layer chromatography (TLC)", 1HNuclear-MagneticsResonances (HNMR), and Fouriere-transforms infrared spectroscopies (FTIR). Characterization demonstrated that pure levan is a homopolysaccharide with just fructose and atypical for carbohydrate bond area of 868 cm-1. The maximum water solubility index (WSI) percentage was 90% at a conc. of 100 mg/5 ml, while the highest water holding capacity value had been 252 at a conc. of 50 mg/5 ml. The wound dressings were prepared using purified levan from L.gasseri and its blend with polyvinyl alcohol (PVA) polymer, and the …

Development And Evaluation Of A Colorimetric Lamp Based-Assay Targeting The Bacteroides Hf183 Marker For Tracking Sewage Pollution In Environmental Waters, Mariah C.A. Do Nascimento, Wendy J.M. Smith, Metasebia Gebrewold, Yawen Liu, Stuart L. Simpson, Aaron Bivins, Paula Rahal, Warish Ahmed

Faculty Publications

Surface waters are vulnerable to contamination by human and animal feces, posing risks to human health due to potential exposure to enteric pathogens. This research developed a colorimetric loop-mediated isothermal amplification (cLAMP) assay to detect sewage associated Bacteroides dorei HF183/BacR287 (HF183) marker in wastewater and environmental water samples. The host sensitivity and host specificity of the assay were evaluated, and their performance was compared to the Bacteroides HF183 qPCR assay using control materials (gBlocks), environmental water samples seeded with untreated sewage, and ambient environmental water samples. In serial dilutions of control materials, qPCR produced quantifiable data across all dilutions, while …

The Utility Of Indocyanine Green Angiography In Breast Reconstruction To Detect Mastectomy Skin Flap Necrosis And Free Flap Perfusion: An Umbrella Review, Nicholas Fadell, Flora Laurent, Sai Anusha Sanka, Esther Ochoa, Lauren Yaeger, Xiaowei Li, Matthew D Wood, Justin M Sacks, Saif Badran

2020-Current year OA Pubs

Two of the greatest challenges in breast reconstruction are mastectomy skin flap necrosis (MSFN) and autologous flap failure. This review summarizes current evidence regarding the usage of indocyanine green angiography (ICGA) in breast reconstruction, identifies knowledge gaps, and provides directions for future studies. An umbrella review was conducted to identify related syntheses in Embase, Ovid Medline, Scopus, the Cochrane Central Register of Controlled Trials, the Cochrane Database of Systematic Reviews, and the Clinical Trials databases. Data were extracted from systematic reviews (SRs) and meta-analyses (MAs) that discussed the use of ICGA in breast reconstruction. Sixteen syntheses were included (10 SRs …

Immuno-Scanning Electron Microscopy Of Islet Primary Cilia, Sanja Sviben, Alexander J Polino, Isabella L Melena, Jing W Hughes

2020-Current year OA Pubs

The definitive demonstration of protein localization on primary cilia has been a challenge for cilia biologists. Primary cilia are solitary thread-like projections that have a specialized protein composition, but as the ciliary structure overlays the cell membrane and other cell parts, the identity of ciliary proteins are difficult to ascertain by conventional imaging approaches like immunofluorescence microscopy. Surface scanning electron microscopy combined with immunolabeling (immuno-SEM) bypasses some of these indeterminacies by unambiguously showing protein expression in the context of the three-dimensional ultrastructure of the cilium. Here, we apply immuno-SEM to specifically identify proteins on the primary cilia of mouse and …

Adipocyte Lipin 1 Expression Associates With Human Metabolic Health And Regulates Systemic Metabolism In Mice, Andrew Lapoint, Jason M. Singer, Daniel Ferguson, Trevor M. Shew, M. Katie Renkemeyer, Hector H. Palacios, Rachael L. Field, Gordon I. Smith, Mai He, Gary J Patti, Samuel Klein, Jonathan R. Brestoff, Brian N. Finck, Et Al.

2020-Current year OA Pubs

Dysfunctional adipose tissue is believed to promote the development of hepatic steatosis and systemic insulin resistance, but many of the mechanisms involved are still unclear. Lipin 1 catalyzes the conversion of phosphatidic acid to diacylglycerol, the penultimate step of triglyceride synthesis, which is essential for lipid storage. Herein we found that adipose tissue LPIN1 expression is decreased in people with obesity compared with lean subjects, and low LPIN1 expression correlated with multi-tissue insulin resistance and increased rates of hepatic de novo lipogenesis. Comprehensive metabolic and multiomic phenotyping demonstrated that adipocyte-specific Lpin1-/- mice had a metabolically unhealthy phenotype, including liver and …

Poshuouinge — City At The Center, All Roads Lead To Poshu, Alice Ruth Gomez

Architecture and Planning ETDs

Poshuouinge was situated in the center of not only the space of the Lower Río Chama Valley, but in the center of the time the Valley was inhabited by Tewa ancestors between the 12th and 16th centuries. Its architecture ends the Coalition Period (AD 1200-1325) and begins the the Classic (AD 1325-1600), a time of population growth and prosperity, trade expansion and diversity, all due to major agricultural innovations unique to the Valley. The new style architecture lent itself to fewer restrictions for neighbors and visitors, and was replicated in some fashion in the Valley’s other nine largest communities. Poshuouinge’s …

Preservice Teachers As Learners, Designers And Implementers Of Computational Thinking (Ldi-Ct), Line Saint-Hilaire, Daisuke Akiba

Publications and Research

Computational thinking (CT) has gained significant recognition in education in recent years. When considering young children learning CT concepts, it's essential to also assess teachers’ understanding of computational thinking. Therefore, educators in teacher preparation programs must equip candidates with these skills to meet the growing demand for developing students’ digital literacy. This project aimed to understand how to strategically incorporate CT instruction into a teacher education program in the Elementary and Early Childhood Education Department. It focuses on assessing preservice teachers' (PSTs) understanding of computational thinking and their ability to design CT-integrated science lesson plans based on their experiences with …

Reproduction Has Immediate Effects On Female Mortality, But No Discernible Lasting Physiological Impacts: A Test Of The Disposable Soma Theory, Sharon E Mitchell, Megan Simpson, Lena Coulet, Solenn Gouedard, Catherine Hambly, Juliano Morimoto, David B Allison, John R Speakman

Children’s Nutrition Research Center Staff Publications

The disposable soma theory (DST) posits that organisms age and die because of a direct trade-off in resource allocation between reproduction and somatic maintenance. DST predicts that investments in reproduction accentuate somatic damage which increase senescence and shortens lifespan. Here, we directly tested DST predictions in breeding and nonbreeding female C57BL/6J mice. We measured reproductive outputs, body composition, daily energy expenditure, and oxidative stress at peak lactation and over lifetime. We found that reproduction had an immediate and negative effect on survival due to problems encountered during parturition for some females. However, there was no statistically significant residual effect on …

Molecular Detection And Genomic Characterization Of Samak Micromys Paramyxovirus-1 And -2 In Micromys Minutus, Republic Of Korea, Augustine Natasha, Sarah E Pye, Carolina B López, Et Al.

2020-Current year OA Pubs

BACKGROUND: The discovery of viruses in small mammalian populations, particularly rodents, has expanded the family Paramyxoviridae. The overlap in habitats between rodents and humans increases the risk of zoonotic events, underscoring the importance of active surveillance. Rodent species, such as Apodemus agrarius, are natural hosts for Paramyxoviridae in the Republic of Korea (ROK). However, it is unknown whether Paramyxoviridae is present in Micromys minutus, another common rodent.

METHOD: Here, we screened M. minutus collected from the Gangwon Province in the ROK for paramyxoviruses using nested polymerase chain reaction and confirm positive samples by next-generation metagenomic sequencing. Complete paramyxovirus genomes were …

“It Is What It Is” – The Lived Experience Of Women With Breast Cancer Undergoing Axillary Lymph Node Dissection, Eva Vikhe Patil, Anna Forsberg, Carina Wennerholm, Jenny Drott

Journal of Patient-Centered Research and Reviews

Purpose: The lived experience of women undergoing axillary procedures as part of their breast cancer (BC) treatment remains unexplored. This lack of in-depth understanding could hamper implementation of person-centred care, which is concerning because BC is the most common form of cancer in women. The aim of this study was therefore to explore the lived experiences of women undergoing axillary lymph node dissection (ALND) due to BC.

Methods: Twelve women with a mean age of 59 years were interviewed about their lived experiences of axillary procedures as part of their BC treatment. The interviews were recorded, transcribed verbatim, and analysed …

Assessing The Climate Readiness Of Physician Education Leaders In Graduate Medical Education, Deborah Simpson, Anne Getzin, Aaron A. Levy, Victoria Gillet

Journal of Patient-Centered Research and Reviews

Climate change affects patient health through an array of exposures, including increasing heatwaves, extreme weather events, poor air quality, and expanding vector-borne illnesses. Physicians are at the forefront of addressing the health consequences of these exposures with patients, and environmental sustainability has become a priority for health care organizations. Accordingly, climate change and health is becoming a critical area for graduate medical education (GME). As GME leaders design and drive education in residency and fellowship programs, understanding those leaders’ baseline knowledge on this topic and its alignment with their organizations’ priorities is an essential step in the development of climate …

United States Centers For Disease Control And Prevention Scientific Integrity Policy

Copyright, Fair Use, Scholarly Communication, etc.

US CDC SIP

Cdk12 Loss Drives Prostate Cancer Progression, Transcription-Replication Conflicts, And Synthetic Lethality With Paralog Cdk13, Jean Ching-Yi Tien, Jie Luo, Yu Chang, Yuping Zhang, Yunhui Cheng, Xiaoju Wang, Jianzhang Yang, Rahul Mannan, Somnath Mahapatra, Palak Shah, Xiao-Ming Wang, Abigail J Todd, Sanjana Eyunni, Caleb Cheng, Ryan J Rebernick, Lanbo Xiao, Yi Bao, James Neiswender, Rachel Brough, Stephen J Pettitt, Xuhong Cao, Stephanie J Miner, Licheng Zhou, Yi-Mi Wu, Estefania Labanca, Yuzhuo Wang, Abhijit Parolia, Marcin Cieslik, Dan R Robinson, Zhen Wang, Felix Y Feng, Jonathan Chou, Christopher J Lord, Ke Ding, Arul M Chinnaiyan

Faculty, Staff and Student Publications

Biallelic loss of cyclin-dependent kinase 12 (CDK12) defines a metastatic castration-resistant prostate cancer (mCRPC) subtype. It remains unclear, however, whether CDK12 loss drives prostate cancer (PCa) development or uncovers pharmacologic vulnerabilities. Here, we show Cdk12 ablation in murine prostate epithelium is sufficient to induce preneoplastic lesions with lymphocytic infiltration. In allograft-based CRISPR screening, Cdk12 loss associates positively with Trp53 inactivation but negatively with Pten inactivation. Moreover, concurrent Cdk12/Trp53 ablation promotes proliferation of prostate-derived organoids, while Cdk12 knockout in Pten-null mice abrogates prostate tumor growth. In syngeneic systems, Cdk12/Trp53-null allografts exhibit luminal morphology and immune checkpoint blockade sensitivity. Mechanistically, Cdk12 inactivation …

Yap1 Status Defines Two Intrinsic Subtypes Of Lcnec With Distinct Molecular Features And Therapeutic Vulnerabilities, C Allison Stewart, Lixia Diao, Yuanxin Xi, Runsheng Wang, Kavya Ramkumar, Alejandra G Serrano, Azusa Tanimoto, B Leticia Rodriguez, Benjamin B Morris, Li Shen, Bingnan Zhang, Yan Yang, Samera H Hamad, Robert J Cardnell, Alberto Duarte, Moushumi Sahu, Veronica Y Novegil, Bernard E Weissman, Michael Frumovitz, Neda Kalhor, Luisa Solis Soto, Pedro Da Rocha, Natalie Vokes, Don L Gibbons, Jing Wang, John V Heymach, Bonnie Glisson, Lauren Averett Byers, Carl M Gay

Faculty, Staff and Student Publications

Purpose: Large cell neuroendocrine carcinoma (LCNEC) is a high-grade neuroendocrine malignancy that, like small cell lung cancer (SCLC), is associated with the absence of druggable oncogenic drivers and dismal prognosis. In contrast to SCLC, however, there is little evidence to guide optimal treatment strategies, which are often adapted from SCLC and non-small cell lung cancer approaches.

Experimental design: To better define the biology of LCNEC, we analyzed cell line and patient genomic data and performed IHC and single-cell RNA sequencing of core needle biopsies from patients with LCNEC and preclinical models.

Results: In this study, we demonstrate that the presence …

Biochemical And Structural Insights Into A 5′ To 3′ Rna Ligase Reveal A Potential Role In Trna Ligation, Yingjie Hu, Victor A Lopez, Hengyi Xu, James P Pfister, Bing Song, Kelly A Servage, Masahiro Sakurai, Benjamin T Jones, Joshua T Mendell, Tao Wang, Jun Wu, Alan M Lambowitz, Diana R Tomchick, Krzysztof Pawłowski, Vincent S Tagliabracci

Faculty, Staff and Student Publications

ATP-grasp superfamily enzymes contain a hand-like ATP-binding fold and catalyze a variety of reactions using a similar catalytic mechanism. More than 30 protein families are categorized in this superfamily, and they are involved in a plethora of cellular processes and human diseases. Here, we identify C12orf29 (RLIG1) as an atypical ATP-grasp enzyme that ligates RNA. Human RLIG1 and its homologs autoadenylate on an active site Lys residue as part of a reaction intermediate that specifically ligates RNA halves containing a 5’-phosphate and a 3’-hydroxyl. RLIG1 binds tRNA in cells and can ligate tRNA within the anticodon loop in vitro. Transcriptomic …

Transcranial Direct Current Stimulation For Improving Balance In Healthy Older Adults And Older Adults With Stroke: A Scoping Review, Shih-Chiao Tseng, Sharon Dunnivan-Mitchell, Dana Cherry, Shuo-Hsiu Chang

Faculty, Staff and Student Publications

Background/Objectives: Age-related decline in balance and postural control is common in healthy elders and is escalated in aging adults with stroke. Transcranial direct current stimulation (tDCS) has emerged as one of the promising brain stimulations adjoining therapeutic exercise to enhance the recovery of balance and motor functions in persons with and without neurological disorders. This review aims to summarize and compare the available evidence of the tDCS on improving balance in the older adults without neurological disorders and the older adults with stroke.

Methods: The Ovid (Medline) database was searched from its inception through to 06/15/2024 for randomized …

Molecular Detection And Genomic Characterization Of Samak Micromys Paramyxovirus-1 And -2 In Micromys Minutus, Republic Of Korea, Augustine Natasha, Sarah E Pye, Carolina B López, Qq Et Al.

2020-Current year OA Pubs

BACKGROUND: The discovery of viruses in small mammalian populations, particularly rodents, has expanded the family Paramyxoviridae. The overlap in habitats between rodents and humans increases the risk of zoonotic events, underscoring the importance of active surveillance. Rodent species, such as Apodemus agrarius, are natural hosts for Paramyxoviridae in the Republic of Korea (ROK). However, it is unknown whether Paramyxoviridae is present in Micromys minutus, another common rodent.

METHOD: Here, we screened M. minutus collected from the Gangwon Province in the ROK for paramyxoviruses using nested polymerase chain reaction and confirm positive samples by next-generation metagenomic sequencing. Complete paramyxovirus genomes were …

The Catholic Imaginations Of J.R.R. Tolkien And Oscar Wilde, Frazier A. Johnson

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

This paper uses terminology from The Catholic Imagination by Andrew Greeley to explore the influence of Catholicism on the writings of Oscar Wilde and J.R.R. Tolkien. Greeley asserts that Catholics see metaphors for the transcendent in matter, value community within a well-ordered hierarchy, and sanctify suffering—qualities that abound in the writings of Wilde and Tolkien. I begin with short biographical sketches of both authors, paying special attention to Wilde’s and Tolkien’s varied involvement with orthodox Catholicism. I then move into a discussion about both authors’ aesthetic philosophies and the similar value they see in art. Next, I detail the authors’ …

“The Company At St. Anne’S” And “The God’S Gardeners”: What C.S. Lewis And Margaret Atwood Teach Us About Caring For Our Planet, Anne-Frédérique Mochel-Caballero

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

At first sight, That Hideous Strength (1945) by C.S. Lewis and the MaddAddam trilogy (2003, 2009, 2013) by Margaret Atwood seem to have little in common. Yet, despite differences of time and place, and approach of the authors, there are startling and generative similarities between the two communities they depict, the Company at St. Anne’s and the God’s Gardeners. In considering the groups' opposition to reductionist technocracy and a shared set of principles, Atwood's MaddAddam trilogy seems to be carrying on the heart of Lewis' dystopic That Hideous Strength as effective ecological and ethical speculative fiction.

The Wart, The Wizard, And The Cathedral: Applying C.S. Lewis's Intertextuality Theory To T.H. White And J.K. Rowling, G. Connor Salter

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

Applying C.S. Lewis' metaphor of authors being cathedral-builders to understand intertextual writing (combining influences with innovation) provides a fascinating lens to understand T.H. White's Arthurian work. Exploring how White's The Once and Future King influenced J.K. Rowling's Harry Potter series (particularly her characters Vernon and Dudley Dursley) provides a particularly interesting example of how fantasy authors use intertextuality to create original works.

Pity, Power, And Tolkien’S Ring: To Rule The Fate Of Many, By Thomas P. Hillman, Giovanni Carmine Costabile

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

No abstract provided.

Reading Tolkien In Chinese: Religion, Fantasy, And Translation, By Eric Reinders, Cait Coker

Mythlore: A Journal of J.R.R. Tolkien, C.S. Lewis, Charles Williams, and Mythopoeic Literature

No abstract provided.

Factors Affecting Plant Sulphur Requirements In New Zealand Pastoral And Arable Systems, M L. Nguyen, K M. Goh

IGC Proceedings (1977-2023)

This paper reviews major factors affecting sulphur (S) cycling in New Zealand pastoral and arable· systems and identifies areas of research required to improve the efficient use of S fertilisers and soil S reserves. In the grazed pastoral system, S removal in animal products constitutes a small fraction of total S output while S leaching losses and excreta! S transfer losses to stock camps are substantial. Although grazing animals may cause a considerable S loss from the system, they enhance the oxidation of elemental S. Accumulated soil organic S due to the return of animal excreta and long-term annual applications …

An Overview Of The New Zealand Experience In Establishing, Maintaining, And Utilizing White Clover Based Pastures, M B. O'Connor, A M. Bryant, R A. Prestidge

IGC Proceedings (1977-2023)

Establishment of white clover (Trifolium repens L.) involves the provision of all major and minor elements necessary for clover growth and N fixation (particularly P, S, K, Mo). Nitrogen fertiliser is not necessary. Inoculation of seed with rhizobium is required where white clover has not been grown previously and in difficult environments. Pest control where necessary is enhanced by insecticidal and nematicidal seed coatings on the white clover seed, Barty management is critical and involves regulnr close grazing during the first 6 months. Maintenance of white clover pastures involves annual inputs of fertiliser (P, S, K) with lime at …

Recent Considerations Of Grassland "Soil Quality" In Temperate Regions, S P. Mcgrath, S C. Jarvis

IGC Proceedings (1977-2023)

The term "soil quality" is increasingly being used in Europe, albeit in an ill-defined way. Perceived quality depends critically on the use being made of the soil and the tolerance of other parts of the environment to substances moving from soils. This paper focuses on issues relevant to the maintenance of quality in temperate grassland soils. Soils are under threat from heavy metals, organic contaminants and radionuclides because they are sinks for many of these substances, thus lending to accumulation. The impact of these contaminants is assessed, along with the management options and relevant legislation to minimise their effects. Most …

Carcinogenicity Of Bracken Fern (Pteridium Spp.) In Relationship To Grazing Ruminants, B L. Smith, D R. Lauren, P P. Embling, M Agnew

IGC Proceedings (1977-2023)

Bracken fern (Pteridium spp.) is the only higher plant which has been shown to cause cancer naturally in animals. Bracken contains ptaquiloside (Pta) a known clastogen and mutagen which is believed to be the major carcinogen involved in the urinary bladder neoplasia. In New Zealand, bracken fern from an area where bovine enzootic haematuria occurs has been shown to have much higher concentrations of Pia than an area where the disease has not been identified. The difference was shown to be related to the bracken rather than the environment and was widespread for the plants in the two areas …

A Slow Release Intra-Ruminal Device For Protection Against Facial Eczema In Sheep, R Munday, E A. Fowke, A M. Thompson, C Wesselink, N R. Towers

IGC Proceedings (1977-2023)

Facial eczema (FE) is a hepatogenous photosensitization disease of ruminants which causes severe losses in agricultural revenue in New Zealand. It is caused by sprophytic, a myotoxin produced by the saprophytic pastoral fungus Pilllomyces chartarum. Zinc (Zn) salts, given to animals before exposure to the toxin, protect against the harmful effects of sporidesmin. Large amounts of Zn are required and dosing must be continued, at regular intervals, throughout the period when animals are exposed to the fungus. While practicable for dairy cattle, these requirements preclude the routine use of Zn for FB prophylaxis in sheep. For these animals, a …