Digital Commons Network^™

Explorations Of Dna-Single-Walled Carbon Nanotube Interactions To Develop Multiplexed Molecularly Specific Biosensors For Inflammation, Amelia K. Ryan

Dissertations and Theses

Inflammatory cytokines such as interleukin-6 (IL-6) and interleukin-12 (IL-12) are central regulators of immune signaling and key biomarkers of disease, yet existing assays for their detection remain slow, invasive, and lack multiplexing ability. This dissertation advances the development of single-walled carbon nanotube (SWCNT) optical nanosensors capable of real-time, multiplexed, and molecularly specific cytokine detection through innovative use of single-stranded DNA (ssDNA) interfaces.

First, an IL-6-specific DNA aptamer was employed as both a dispersing agent and recognition probe for SWCNT fluorescence sensing. Sequence modifications, including anchor domains, truncations, and thermally induced refolding, were systematically tested to optimize sensitivity and selectivity. The …

Advancing Electrical Stimulation: Full-Head Mri Segmentation For Abnormal Brain Anatomy With Tdcs, Andrew Birnbaum

Dissertations and Theses

Evaluating the effectiveness of transcranial direct current stimulation (tDCS) is essential for guiding its integration into therapeutic and performance-enhancement applications. In our laboratory, we investigate the efficacy of tDCS across multiple experimental models, including both animal and human studies. I have contributed significantly to the execution and analysis of these experiments, which include studies in rats and healthy human participants aimed at evaluating whether electrical stimulation of the motor cortex can enhance motor learning. These studies assess improvements in fine motor performance resulting from tDCS. In stroke patients, I contribute to our investigation of tDCS as a rehabilitative intervention, particularly …

Wearable Disposable Elecetrotherapy, Mohamad Fallahrad

Dissertations and Theses

Non-invasive electrotherapies offer promising treatments for a wide range of conditions such as pain, headaches, depression, addiction, cognitive decline, wound healing, and drug delivery. However, patient compliance remains low due to the high initial cost, cumbersomeness and inconvenience of existing technology compared to pharmaceuticals. Current electrotherapy devices are often bulky and require users to connect disposable electrodes, initiate programs, and manage charging cycles, which hinders widespread adoption.

We address these challenges by designing and validating a novel electrotherapy platform that is single-use, disposable, low-cost, and wearable. Activated automatically upon skin contact, the device delivers a preset dose of controlled electrical …

Utilizing Deep Learning Audio Models For Blind And Low Vision Crosswalk Assistance, Wayne Lam

Dissertations and Theses

Navigating urban environments poses significant challenges for blind and low vision (BLV) individuals, particularly at street intersections where determining when it is safe to cross can be life-threatening. In New York City, where pedestrian fatalities are on the rise and only 2% of intersections are equipped with Accessible Pedestrian Signals (APS), alternative solutions are urgently needed. This thesis proposes an audio-based deep learning approach to support BLV individuals at crosswalks by detecting traffic movement direction and idling states using spatial sound. With 4-channel audio capturing capabilities of wearables, such as Meta Project Aria glasses, we explore state-of-the-art sound event localization …

Multi-Material Adhesive Bonded Joints Under Static And Dynamic Loading, Gizem Derya Demir

Dissertations and Theses

Adhesively bonded joints present significant potential for reducing structural weight and enhancing performance in modern and future engineering structures. By enabling the elimination or reduction of traditional mechanical fasteners and facilitating the integration of dissimilar materials, adhesives can contribute to more efficient, lightweight designs. However, to fully leverage these benefits—especially in high-performance applications—adhesive materials must exhibit robust behavior under both static and dynamic loading conditions, such as ballistic impact. Despite their promise, the failure mechanisms of adhesives under such high-strain-rate scenarios remain insufficiently understood.

This dissertation investigates the performance of adhesively bonded multi-material joints subjected to static loading and ballistic …

Generation And Characterization Of Human Blood-Brain Barrier Models For Investigating Neuropsychiatric Disorders And Tumor Metastasis, Yunfei Li

Dissertations and Theses

The blood–brain barrier (BBB) is important in the normal function of the central nervous system (CNS). An altered BBB has been described in various neuropsychiatric disorders. The brain-specific microvascular endothelial cell (BMEC) is an essential structural component of the BBB. To test if integrity of the BBB formed by BMECs is compromised in 22q11.2 deletion syndrome (also called DiGeorge syndrome), which is one of the validated genetic risk factors for schizophrenia, a 2D iBBB (induced BBB) on a Transwell filter was generated from human microvascular endothelial cells (HBMECs) derived from the induced pluripotent stem cells (iPSCs) out of patients with …

Antibiotic-Resistant Nontyphoidal Salmonella And Campylobacter Isolates Associated With International Travel, Connecticut 2010 – 2023, Shilpa Mudumbe

Public Health Theses

Antibiotics are helpful for limiting duration and complications of Salmonella andCampylobacter isolates in selected age groups and people who are immunosuppressed. However, antibiotic-resistant (AR) foodborne pathogens have been on the rise in the United States over the past 10 years and are often linked to international travel (IT). Long-term trends on AR have not been examined in Connecticut (CT). This study evaluates trends in AR among nontyphoidal Salmonella and Campylobacter isolates in CT from 2010 to 2023 and examines the association between AR and IT. Data from submission of annual systematic samples of CT Salmonella and Campylobacter isolates to the …

The Relevance Of Natural Language Versus Terms And Connectors Searching In Westlaw And Lexis, Amy Taylor

Scholarly Works

Thirty years have passed since a published comparison of natural language and terms and connectors searching in legal databases, and that analysis included only Westlaw. The current study compares natural language and terms and connectors searching in both Westlaw and Lexis for two hypothetical scenarios. Searchers ran four searches for each hypothetical: Lexis natural language, Westlaw natural language, Lexis terms and connectors, and Westlaw terms and connectors. The results showed no statistically significant difference between natural language and terms and connectors for either hypothetical. This suggests it may be appropriate to reduce the time and energy spent teaching law students …

Period Poverty And The Politics Of Refusal: Building Menstrual Equity Beyond The State Through Mutual Aid, Alanah Armstead

Public Health Theses

This thesis examines period poverty as a consequence of systemic neglect, disproportionately affecting those made vulnerable by intersecting structures of racial, gendered, and economic injustice. Situating menstruation as a public health crisis shaped by policy failure, social stigma, and institutional disregard, this research critiques dominant legal and policy frameworks that approach menstrual equity through a limited scope that overlooks deeper structural inequalities rooted in race and class. This thesis explores how marginalized populations experience legal and welfare systems as sites of surveillance and moral judgment in the context of menstrual equity. Through seven semi-structured interviews with menstrual justice organizers, this …

Laro Kwo-Saving Lives: Evaluating The Role Of Community Health Workers In Healthcare Delivery And Disease Control In Northern Uganda, Brett Albee

Public Health Theses

Background: Northern Uganda faces persistent health challenges due to a legacy of armed conflict, poverty, and fragile health infrastructure. In Pader District, malaria remains the leading cause of under-five mortality, and rural communities face ongoing barriers to accessing timely care. Community health workers (CHWs) serve as critical intermediaries in care delivery, but their effectiveness is limited by structural and resource constraints.Methods: This study employed an exploratory sequential mixed-methods design informed by community-based participatory research (CBPR) principles. Qualitative data were collected through 11 semi-structured interviews and 6 focus group discussions with CHWs across six sub-counties in Pader District. Quantitative data were …

Assessing The Costs Of Illness Attributable To Leptospirosis, Alyssa Chetrick

Public Health Theses

Leptospirosis, caused by the spirochetes of the genus Leptospira, is a zoonotic bacterialdisease endemic to tropical regions and lower middle income countries (LMICs). Leptospirosis causes an estimated 1 million cases and more than 58,900 deaths annually.(Costa et al., 2015; Torgeson et al., 2015) In addition to human health, leptospirosis significantly impacts animal health and the livestock industry, particularly through reproductive losses, increased treatment costs, and decreased milk production, which exacerbates global insecurity.(Bennet, 2015; Torgeson et al., 2015) The estimated global cost loss to productivity due to disease is estimated at Int$ 29.3 billion. (Agampodi et al., 2023) Because of its …

The Impact Of Climate Change On Japanese Encephalitis Trends In China, Jiayi Du

Public Health Theses

Japanese encephalitis (JE) is a mosquito-borne viral disease that poses a significant public health threat across Asia, including China. Climate change has been increasingly recognized as a key driver of JE transmission dynamics, influencing mosquito vector distribution and virus proliferation. This study aimed to assess how climatic variables affect the ecological suitability for Japanese encephalitis virus (JEV) transmission, using species distribution modeling with MaxEnt.

Using climate data from WorldClim and JE incidence data from published sources, we applied the MaxEnt model to identify the key environmental predictors of JE transmission and project future risk areas under changing climatic conditions. The …

The Evolution And Effectiveness Of Sterile Insect Technique For Vector Control: A Historical And Modern Analysis, Katerina Karaiskos

Public Health Theses

Sterile Insect Technique (SIT) has emerged as an important tool in the control of vector-borne diseases, providing an alternative to traditional chemical insecticides. Initially based on the irradiation of insects to induce sterility, SIT has proven effective in eradication campaigns, including the cases of the screwworm and tsetse fly. SIT’s primary strengths lie in its species specificity, environmental safety, and the avoidance of pesticide resistance. However, “classic” irradiating SIT is limited by high operational costs, reduced mating competitiveness of sterilized males, and difficulties in addressing high-density vector populations. Recent advancements in genetic-based SIT methods, such as the Release of Insects …

Assessing The Cross-Species Vaccine Potential Of Pfripr Using Transgenic Chimeric Plasmodium Knowlesi Models, Peiqi Li

Public Health Theses

Malaria remains a major global health burden, with an estimated 263 million cases and597,000 deaths reported in 83 countries in 2023 (1). While P. falciparum is responsible for the most severe cases, non-falciparum species such as P. vivax, P. ovale curtisi, P. ovale wallikeri, P. malariae, and P. knowlesi also pose significant public health risks, particularly in co-endemic regions. Current malaria vaccine efforts mainly focus on P. falciparum which is reasonable because over 90% of the global malaria-related morbidity and mortality are caused by P. falciparum (2). However, laying emphasis on targeting P. falciparum leaves a critical gap in cross- …

Global Perspectives On 1,4-Dioxane: Environmental Persistence, Detection Strategies, And Policy Frameworks, Haoyu Lyu

Public Health Theses

1,4-Dioxane is a persistent environmental contaminant present in industrial activities, pharmaceutical manufacturing, and consumer items, particularly challenging to manage due to its high solubility in water and resistance to traditional water treatment processes. Although some countries have implemented stringent regulatory measures, there is a lack of consistent detection and remediation of 1,4-Dioxane in the environment, potentially leading to unregulated contamination in areas with insufficient monitoring capabilities.

In this literature review, studies that have already been published focusing on 1,4-dioxane pollution in China, South America (including Brazil, Argentina, and Peru), and Europe (Germany and the Netherlands) will be evaluated against relevant …

Evaluating The Implications Of Class And Regional Stereotypes On Attributions Of Blame During Hurricane Helene: A Reflexive Content Analysis Of Tiktok, Abby Ong

Public Health Theses

Background and Objectives: Hurricane Helene devastated entire towns across the Southern and Appalachian U.S. Following the hurricane, people who lived in impacted regions were derided for their perceived failures in evacuating. Utilizing Bernard Weiner’s attribution theory of social motivation, this study sought to analyze potential implications of regional stereotypes on how people blamed or supported disaster victims. Primary objectives were to: (1) to analyze content regarding residents’ decisions to evacuate or not evacuate during the storm, (2) to document attributions of blame or support and how those experiencing the disaster and outside observers responded to these attributions, and (3) to …

New Horizons In Hepatitis B Therapy: Antigen Reduction And Immune Modulation Approaches, Yiyun Pan

Public Health Theses

Chronic hepatitis B (CHB) remains a significant global health burden despite advances in antiviral therapy. Current treatments, including nucleoside analogs (NAs) and pegylated interferon (Peg-IFN), effectively suppress viral replication but rarely achieve functional cure, defined as sustained hepatitis B surface antigen (HBsAg) loss. Emerging therapeutic strategies focus on antigen reduction and immune modulation to overcome the limitations of existing therapies.

Antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) offer promising approaches by directly targeting viral RNA to reduce HBsAg levels. Clinical trials of ASOs such as bepirovirsen and siRNAs such as JNJ-3989 and VIR-2218 have shown substantial HBsAg declines, but …

Liver Epigenetic Changes In Mice Exposed To 1,4-Dioxane In The Drinking Water: Implications For 1,4-Dioxane Liver Carcinogenicity, Shuya Qiao

Public Health Theses

1,4-Dioxane, a probable human carcinogen and widespread environmental contaminant, has been consistently associated with liver tumor development in rodents. However, limited research has addressed its role in epigenetic dysregulation. This study investigated epigenetic alterations in liver tissue following chronic exposure to 1,4-dioxane. Male mice of wild-type (WT) and glutamate-cysteine ligase modifier subunit knockout (Gclm-KO) genotypes were exposed to 0, 50, or 500 ppm of 1,4-dioxane in drinking water for six months. qPCR, Western blotting, and immunohistochemistry (IHC) were used to assess changes in DNA methylation-related enzyme gene expression, histone acetylation, and SAM (S-adenosylmethionine) distribution. qPCR results showed significant downregulation of …

Assessing Environmental And Sociodemographic Risk Factors For Leptospira Infection In A Urban Informal Settlements: A Study In Salvador, Brazil, Sebastian Alonso Salazar

Public Health Theses

Background: Leptospirosis is a neglected zoonotic disease that disproportionately affects people living in areas with poor sanitation, water contamination and rodent infestation such as urban informal settlements. More than one billion people worldwide reside in such communities, and with increasing urbanization, coupled with environmental change, the risk of Leptospira transmission continues to increase. Despite its global burden, this disease is often underreported due to diagnostic challenges and limited healthcare access, particularly in urban informal settlements. Understanding the incidence and risk factors in these settings is essential for improving public health interventions. This study aims to estimate the annual incidence of …

Epidemiological Changes In Shiga Toxin-Producing Escherichia Coli (Stec) Pre- And Post-Covid In Connecticut, 2018–2019 And 2023–2024, Brooke Thanh-Hang Tran

Public Health Theses

Background: Shiga toxin-producing Escherichia coli (STEC) causes over 265,000 infections, 3,600 hospitalizations, and 30 deaths annually in the U.S.. While STEC O157 is the most common serotype, the increased use of culture-independent diagnostic tests (CIDT) led to a rise in non-O157 serotypes detected. Nationally, STEC incidence declined during the COVID-19 pandemic but has since surpassed pre-pandemic levels. An analysis was conducted to determine the changes that occurred in STEC infections in Connecticut from pre-pandemic to post-pandemic and to identify differences between culture-confirmed cases and CIDT-only/untyped cases.Methods: A descriptive analysis using STEC surveillance data collected in Connecticut was performed to identify …

Expanding The Trypanosoma Brucei Parasite Detection Limit Through Tb177 Gene Detection In Qpcr, Yi Yan

Public Health Theses

Trypanosomiasis, caused by protozoan parasites of the Trypanosoma genus, remains a significant health and veterinary burden in sub-Saharan Africa, with Human African Trypanosomiasis (HAT) and Animal African Trypanosomiasis (AAT) posing ongoing threats to underserved populations. Despite advances in therapeutics, the lack of sensitive and scalable diagnostic tools continues to limit early detection, effective disease management, and evaluation of emerging vaccine candidates. Current diagnostic methods such as light microscopy and serological tests frequently fail to detect low levels of parasitemia during the early stages of infection, thereby delaying intervention and increasing the risk of disease progression and transmission.

Our study mainly …

Age-Associated Alterations In Immune And Inflammatory Responses In Captive Olive Baboons (Papio Anubis), Michele M. Mulholland, Bharti P. Nehete, Ashley Delise, Angela M. Achorn, Lisa M. Pytka, Pramod N. Nehete

Anthropology Faculty Publications

Introduction: Advanced age is a primary risk factor for many chronic diseases and conditions; however, age-related immune dysregulation is not well understood. Animal models, particularly those that resemble human age-related physiological changes, are needed to better understand immunosenescence and to improve health outcomes. Here, we explore the utility of the olive baboon (Papio anubis) in studying age-related changes to the immune system and understanding mechanisms of immunosenescence.

Methods: We examined immune cell, inflammatory responses, cytokines, and cortisol levels using hematology and flow cytometry, mitogen stimulation, multiplex cytokine assay, and cortisol immunoassay.

Results and Discussion: Our results reveal significant age effects …

The Transformative Power Of An Ethic Of Care In Patricia Piccinini’S Mythic Posthuman Offspring, Keely Parrish

Dissertations and Theses

This thesis examines the contemporary artist Patricia Piccinini and her posthuman hybrid creations within the context of a feminist ethic of care. Largely referring to scholarship by the political scientist Joan C. Tronto, I argue that Piccinini expands the definition of an ethic of care to include non-human entities as well as human ones. The four phases of care identified by Tronto will serve to outline the encounter between the audience and Piccinini’s work to see how humans and non-humans (1) care about, (2) take care of, (3) give care and (4) receive care from each other, as well as …

Revelations: Spirituality As Privileged Perspectivism In Magical Realist Distortions, Isabella Scala-Kazanecki

Dissertations and Theses

This thesis examines the role of spiritual beliefs in the literary mode now known as magical realism in Cuban Alejo Carpentier’s The Kingdom of This World (1949), Indian-Born British and American Salman Rushdie’s The Satanic Verses (1988), and a lesser-known novel, Household Saints (1981) by Francine Prose, an American writer. The Kingdom of This World, itself the originator of the mode of magical realism, has allowed me to conclude with Carpentier that magical realism arises from “privileged revelations,” as he notes in the preface to his seminal book. But these revelations in magical realism are not necessarily always of the …

Isolating Confounding Abiotic And Biotic Effects On The Phenotype On Physa Acuta Snails, Ashley M. Bauer

Theses and Dissertations

Organisms match their phenotypes to prevailing environmental conditions leading to habitat specific polymorphisms. Polymorphisms arise from genetic divergence, phenotypic plasticity, or a combination of both processes. I examined the presence of habitat-specific polymorphism in Physa acuta snails collected from three streams and their corresponding downstream reservoir. In lotic systems shells were rotund with larger apertures, while those from lentic systems were more elongate with smaller apertures. In a reciprocal transplant lab experiment that crossed flow regime and crayfish predator cues, I tested for the underlying mechanisms responsible for the observed phenotypic differences. I found no evidence for plasticity in relation …

Comparative Analysis Of Lipid Accumulation And Antioxidant Responses In Masld And Control Ipsc-Heps, Anna Werts

CMC Senior Theses

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a prevalent liver condition characterized by lipid accumulation and associated inflammation, driven by genetic, environmental, and metabolic factors. Much is still unknown about the mechanisms of the disease, and research on this is important for advancing effective treatments. This study investigated lipid accumulation and oxidative stress responses to varying concentrations of oleic acid (OA) and palmitic acid (PA) in induced pluripotent stem cell-derived hepatocytes (iPSC-Heps) from MASLD and healthy donors. BODIPY staining revealed that MASLD iPSC-Heps exhibit significantly higher lipid accumulation than controls, especially under prolonged OA and PA exposure. Additionally, PLIN2 expression …

New Genetic Tools To Define The Pathophysiology Of Inborn Errors Of Cobalamin Metabolism Impacting Mammalian Development, Tiffany Chern, Xuefei Tong, William G Bauer, David J Quispe-Parra, Xia Gao, Kamryn N Gerner-Mauro, Ross A Poché

Children’s Nutrition Research Center Staff Publications

The congenital, autosomal recessive disorder combined methylmalonic acidemia and homocystinuria - cblC type, is the most common inborn error of cobalamin (vitamin B12) metabolism. In its early onset form, cblC profoundly impacts fetal development of the central nervous system, hematopoietic system, and other tissues. Previously, mutations in the MMACHC gene, which encodes a protein required for the intracellular trafficking and enzymatic processing of free cobalamin into active coenzyme forms, were found to cause cblC. These coenzymes are required in two metabolic pathways which produce either succinyl-CoA in the mitochondria or methionine in the cytosol. However, due to a lack of …

Lawyers And Public Service: Duty, Faith, And The 'Good Republican' In The West Wing, Andrew Flavelle Martin

Articles, Book Chapters, & Popular Press

Popular culture reveals much about the perceived role of lawyers in contemporary life. In this article, I draw lessons from the portrayal of lawyers in Aaron Sorkin's classic television series, The West Wing. As a drama centred around a Democratic presidential administration, Republicans often provide the foil. From time to time, however, the show lionizes what might be termed ‘the good Republican’. That ‘good Republican’ is most often a practicing lawyer whose desire to serve is grounded in duty or faith. In this essay, I use a trio of these characters to explore the role of lawyers in public service. …

A Reflection-Spectroscopy Measured Skin Carotenoid Score Strongly Correlates With Plasma Concentrations Of All Major Dietary Carotenoid Species Except For Lycopene, Qiang Wu, Cheryl Webb Cherry, Stephanie Jilcott Pitts, Melissa N Laska, Neal Craft, Nancy E Moran

Children’s Nutrition Research Center Staff Publications

Skin carotenoids can be measured non-invasively using spectroscopy methods to provide a biomarker of total dietary carotenoid and carotenoid-rich fruit and vegetable intake. However, the degree to which skin carotenoid biomarkers reflect intakes of specific carotenoids must be determined for specific devices. Previously, findings were mixed regarding the correlation between reflection spectroscopy (RS)-assessed skin carotenoids and individual plasma carotenoid concentrations. The current study expands on prior analyses to examine the cross-sectional associations between adult RS-assessed skin carotenoids and individual carotenoid species intakes and plasma concentrations, controlling for potential covariates. We hypothesized that RS-assessed skin carotenoid scores would strongly correlate with …

Is This Cultural Property Law?: The Emerging Norm Of Voluntary Repatriations, Erin L. Thompson

Chapman Law Review

As the public’s awareness of the histories of theft and smuggling that brought many cultural artifacts from their communities of origin to American collections has grown, attitudes toward the ethics of retaining these artifacts has also shifted. This presentation will first consider challenges posed by existing legal remedies available for source countries who seek to reclaim their heritage and then discuss the emerging practice of voluntary repatriations, which occur when the current owner of an artifact returns it to a source country or community even though legal authorities would likely not compel its surrender.