Digital Commons Network^™

Innate Immune Sensing Of Rotavirus By Intestinal Epithelial Cells Leads To Diarrhea, Gaopeng Hou, Juhee Son, Maria Florencia Gomez Castro, Takahiro Kawagishi, Avan Antia, Qiru Zeng, Anna L Deveaux, Hinissan P Kohio, Megan T Baldridge, Siyuan Ding, Et Al.

2020-Current year OA Pubs

Diarrhea is the predominant symptom of acute gastroenteritis resulting from enteric infections and a leading cause of death in infants and young children. However, the role of the host response in diarrhea pathogenesis is unclear. Using rotavirus and neonatal mice as a model, we found that oral inoculation of UV-inactivated replication-defective rotavirus consistently induced watery diarrhea by robust activation of cytosolic double-stranded RNA sensing pathways and type III interferon (IFN-λ) secretion. Diarrhea was significantly diminished in mice lacking the IFN-λ receptor. Mechanistically, IFN-λ signaling downregulates the expression of Dra, a chloride and bicarbonate exchanger, which contributes to reduced water absorption. …

A Comparison Of Unconventional Microwave And Ultrasound-Assisted Extraction Methods Used For Flavonoids, Istiqomah Rahmawati, Daffa Hafiziaulhaq Azizi, Jihan Nafila Wibowo, Muhammad Reza, Boy Arief Fachri, Bekti Palupi, Meta Fitri Rizkiana, Helda Wika Amini, Ifan Ramadana, Felix Arie Setiawan

Makara Journal of Science

Cocoa pods (Theobroma cacao L.) are a rich source of flavonoids, which are natural antioxidants known for their health benefits. This study investigated the use of microwave-assisted extraction (MAE) and ultrasound-assisted ex-traction (UAE) to extract the maximum flavonoids and antioxidants from cocoa pods. MAE and UAE are efficient and sustainable methods for extracting bioactive compounds like flavonoids and antioxidants from cocoa pods, offer-ing faster extraction, reduced solvent use, and better compound preservation compared to conventional methods. These technologies unlock the untapped potential of cocoa pods for applications in food, cosmetics, and pharmaceuti-cals. The effects of extraction time (2–10 min), microwave …

2025 Nebraska Farmland Values And Cash Rental Rates, Jim Jansen

Cornhusker Economics

The market value of agricultural land in Nebraska declined by 2% over the prior year to an average of $3,935 per acre, according to the 2025 Nebraska Farm Real Estate Market Survey. This marks the first decline in the market value of agricultural land in Nebraska since reaching the record non-inflation-adjusted statewide land value in the survey's 46-year history in 2024.

The University of Nebraska-Lincoln’s Department of Agricultural Economics annually surveys land industry professionals across Nebraska, including appraisers, farm and ranch managers, agricultural bankers, and related industry professionals. Results from the survey are divided by land class and summarized by …

Cost-Effective Active Laser Scanning System For Depth-Aware Deep-Learning-Based Instance Segmentation In Poultry Processing, Pouya Sohrabipour, Chaitanya Kumar Reddy Pallerla, Amirreza Davar, Siavash Mahmoudi, Philip Crandall, Wan Shou, Yu She, Dongyi Wang

School of Industrial Engineering Faculty Publications

The poultry industry plays a pivotal role in global agriculture, with poultry serving as a major source of protein and contributing significantly to economic growth. However, the sector faces challenges associated with labor-intensive tasks that are repetitive and physically demanding. Automation has emerged as a critical solution to enhance operational efficiency and improve working conditions. Specifically, robotic manipulation and handling of objects is becoming ubiquitous in factories. However, challenges exist to precisely identify and guide a robot to handle a pile of objects with similar textures and colors. This paper focuses on the development of a vision system for a …

Climate Change Policies And Good Governance In Egypt, Mahmoud Elshourbagy

Theses and Dissertations

Egypt recognizes the impacts of climate change and is particularly vulnerable because of its high-density population, economic burden and geographical location. In response, Egypt established National Climate Change Council in 2016. In 2022, the council issued “National Climate Change Strategy 2050” to integrate and align the efforts of government bodies to adapt and mitigate climate change risks. One of the strategic goals of “National Climate Change Strategy 2050” is to enhance climate governance in Egypt as a critical tool. This study attempts to evaluate climate change policies in the context of good governance by using a tool developed by World …

Spectrum, Volume 54, Issue 6, Sacred Heart University

Newspapers (Obelisk & Spectrum)

Highlights include: SHU Health Science Class Partners with the CT Mirror - SHU Professor and Student Collaborate on Children’s Book - Senior Week 2025: The Final Show, Off-Campus Food Finds - Celebrating Saint Patrick’s Day in Connecticut - The Stage is Set for TheatreFest Round Three - Short Filmmakers Club Showcases Reel Talent - Men’s Hockey Advances to AHA Semifinals

Effects Of Space Flight On Inflammasome Activation In The Brain Of Mice, Upal Roy, Roey Hadad, Angel A. Rodriguez, Alen Saju, Deepa Roy, Mario Gil, Robert W. Keane, Ryan T. Scott, Xiao W. Mao, Juan Pablo De Rivero Vaccari

Health & Biomedical Sciences Faculty Publications

Space flight exposes astronauts to stressors that alter the immune response, rendering them vulnerable to infections and diseases. In this study, we aimed to determine the levels of inflammasome activation in the brains of mice that were housed in the International Space Station (ISS) for 37 days. C57BL/6 mice were launched to the ISS as part of NASA’s Rodent Research 1 Mission on SpaceX-4 CRS-4 Dragon cargo spacecraft from 21 September 2014 to 25 October 2014. Dissected mouse brains from that mission were analyzed by immunoblotting of inflammasome signaling proteins and Electrochemiluminescence Immunoassay (ECLIA) for inflammatory cytokine levels. Our data …

Comprehensive Review On The Application Of Bio-Immunoinformatics In The Development Of Highly Ef-Fective New Candidate Vaccines Against Tuberculosis, Ahyar Ahmad, Andriansjah Rukmana, Miski A. Khairinisa, Dian A. E. Pitaloka, Rosana Agus, Rusdina B. Ladju, Tarwadi Ahmad, Astutiati Nurhasanah, Carina C. D. Joe, Muhammad N. Massi, Harningsih Karim, Irda Handayani, Siti Roszilawati Binti Ramli

Karbala International Journal of Modern Science

Tuberculosis (TB) remains a significant public health challenge worldwide. Currently, Bacillus Calmette-Guerin (BCG) is the only vaccine available for TB prophylaxis. However, the efficacy of the BCG vaccine against adult pulmonary TB is considered inconsistent. This condition encourages researchers to look for more effective options, such as subunit vaccines. This condition requires the development of a more effective subunit vaccine to protect active TB in productive and adult ages. There is an urgent need for more effective vaccines, as the Bacillus Calmette-Guérin (BCG) vaccine currently available has inconsistent efficacy and is only partially effective in adults. Bio-immunoinformatics, an interdisciplinary field …

Articulated Robot Path Planning Based On Hybridization Of Adaptive ‎Dimensionality Algorithm And Grey Wolf Optimizer In Dynamic Environments, Noor Kadhim Ayoob‏, Ali Hadi Hasan

Karbala International Journal of Modern Science

A new method was developed to plan a path for a robotic articulated vehicle using the Grey Wolf Optimizer (GWO) ‎and Adaptive ‎Dimensionality (AD)‎. Existing studies in robotics path planning ignore the differences between robots in terms of size and flexibility and allocate a single cell to the robot regardless of the mentioned factors. Since the articulated robotic vehicle is longer than obstacles moving in the environment, this study takes into account vehicle size and flexibility in path planning by adapting the number of cells allocated to the robotic vehicle to contain the vehicle parts while performing different movements. Considering …

Ras El-‘Amud, Jerusalem: Faunal Remains From The Intermediate Bronze And Middle Bronze Age Settlement, Nimrod Marom

Qadum: Journal of Excavation Reports from Israel

The small faunal assemblage from Ras el-‘Amud provides insights into the economic strategies of a rural settlement during a period of urban growth. A shift from a self-sufficient goat and pig economy to a specialized sheep-focused one is observed, likely driven by urban demand. Analysis of caprine skeletal remains suggests consumption patterns and potential ritualistic practices. The Middle Bronze IIA village exhibits a decrease in butchery waste, possibly due to changes in spatial organization or waste disposal practices. The assemblage offers valuable insights into the economic and social dynamics of rural settlements during the Middle Bronze Age.

Ras El-‘Amud, Jerusalem: The Groundstone Tool Assemblage, Aaron Greener

Qadum: Journal of Excavation Reports from Israel

The excavations at Ras el-‘Amud yielded 71 identified groundstone tools, 55 of which were discovered in the Intermediate Bronze Age (IBA) strata, with the remaining 16 found in the Iron Age and Roman/Byzantine strata and in unclear contexts. These tools mainly comprise hammerstones, likely associated with the production of flint tools at the site, as well as perforated stones, mortars, bowlets, rubbing stones, a grinding stone, a pestle and some other tools. The identified groundstone tools suggest activities related to the processing of cereals, grains and other agricultural produce, as well as possible animal fiber processing and various domestic tasks …

Patterns And Representation In Play-Based Learning: A Systematic Meta-Synthesis Of Empirical Studies In K-13+ Settings, Steph Dean, Julianne A. Wenner

Publications

This systematic review provides a comprehensive meta-synthesis that examines empirical research on the implementation of play-based learning in formal educational settings. The review aims to identify patterns in the enactment of play-based learning, including its representation across academic disciplines, methodologies, grade levels, geographic contexts, and key indicators of play—choice, wonder, and delight. A comprehensive search across nine databases yielded 1,475 studies, of which 87 met stringent inclusion criteria: empirical, K-13+ formal settings, and an intentional learning objective tied to play. We extracted data from each study and used thematic synthesis to analyze patterns across multiple dimensions. Findings indicate that play-based …

Exploring The Impacts Of Food System Education: A Case Study, Constanza Arevalo Msc, Andie Thompkins Ph.D.

Diet

This case study, involving qualitative and quantitative methods, explores the long-term impacts of an education program that trains students in effective communication, advocacy, and leadership skills to combat factory farming. The results from this study will shed light on the effectiveness and value of educational programs for the animal protection movement and for the students who partake in them.

Necrosis Drives Susceptibility To Mycobacterium Tuberculosis In Polg(D257a) Mutator Mice, C J Mabry, C G Weindel, L W Stranahan, Jordyn J Vanportfliet, J R Davis, E L Martinez, A Phillip West, K L Patrick, R O Watson

Faculty Research 2025

The genetic and molecular determinants that underlie the heterogeneity of Mycobacterium tuberculosis (Mtb) infection outcomes in humans are poorly understood. Multiple lines of evidence demonstrate that mitochondrial dysfunction can exacerbate mycobacterial disease severity, and mutations in some mitochondrial genes confer susceptibility to mycobacterial infection in humans. Here, we report that mutations in mitochondria DNA (mtDNA) polymerase gamma potentiate susceptibility to Mtb infection in mice. PolgD257A mutator mtDNA mice fail to mount a protective innate immune response at an early infection time point, evidenced by high bacterial burdens, reduced M1 macrophages, and excessive neutrophil infiltration in the lungs. Immunohis tochemistry reveals …

Pgrmc2 Is A Pressure-Volume Regulator Critical For Myocardial Responses To Stress In Mice, Farideh Amirrad, Vivian La, Sharareh Ohadi, Miram Albotaif, Sha Webster, James K. Pru, Kiumars Shamloo, Ashraf M. Mohieldin, Surya M. Nauli

Pharmacy Faculty Articles and Research

Progesterone receptors are classified into nuclear and membrane-bound receptor families. Previous unbiased proteomic studies indicate a potential association between cardiac diseases and the progesterone receptor membrane-bound component-2 (PGRMC2); however, the role of PGRMC2 in the heart remains unknown. In this study, we use a heart-specific knockout (KO) mouse model (MyH6•Pgrmc2^flox/flox) in which the Pgrmc2 gene was selectively deleted in cardiomyocytes. Here we show that PGRMC2 serves as a mediator of steroid hormones for rapid calcium signaling in cardiomyocytes to maintain cardiac contraction, sufficient stroke volume, and adequate cardiac output by regulating the cardiac pressure-volume relationship. The KO …

Recessive Genetic Contribution To Congenital Heart Disease In 5,424 Probands, Weilai Dong, Sheng Chih Jin, Et Al.

2020-Current year OA Pubs

Variants with large effect contribute to congenital heart disease (CHD). To date, recessive genotypes (RGs) have commonly been implicated through anecdotal ascertainment of consanguineous families and candidate gene-based analysis; the recessive contribution to the broad range of CHD phenotypes has been limited. We analyzed whole exome sequences of 5,424 CHD probands. Rare damaging RGs were estimated to contribute to at least 2.2% of CHD, with greater enrichment among laterality phenotypes (5.4%) versus other subsets (1.4%). Among 108 curated human recessive CHD genes, there were 66 RGs, with 54 in 11 genes with >1 RG, 12 genes with 1 RG, and …

Transcriptional Profiles Reveal Physiological Mechanisms For Compensation During A Simulated Marine Heatwave In Yellowtail Kingfish (Seriola Lalandi), Sharon E. Hook, Ryan J. Farr, Jenny Su, Alistair J. Hobday, Catherine Wingate, Lindsey Woolley, Luke Pilmer

Fisheries Research Articles

Background

Changing ocean temperatures are already causing declines in populations of marine organisms. Predicting the capacity of organisms to adjust to the pressures posed by climate change is a topic of much current research effort, particularly for species we farm or harvest. To explore one measure of phenotypic plasticity, the physiological compensations in response to heat stress as might be experienced in a marine heatwave, we exposed Yellowtail Kingfish (Seriola lalandi) to sublethal heat stress, and used the transcriptome in gill and muscle, benchmarked against heat shock proteins and oxidative stress indicators, to characterise the acute heat stress …

Structural Basis For Intrinsic Strand Displacement Activity Of Mitochondrial Dna Polymerase, Ashok Nayak, Viktoriia Sokolova, Sirelin Sillamaa, Karl Herbine, Juhan Sedman, Dmitry Temiakov

Department of Biochemistry and Molecular Biology Faculty Papers

Members of the Pol A family of DNA polymerases, found across all domains of life, utilize various strategies for DNA strand separation during replication. In higher eukaryotes, mitochondrial DNA polymerase γ relies on the replicative helicase TWINKLE, whereas the yeast ortholog, Mip1, can unwind DNA independently. Using Mip1 as a model, we present a series of high-resolution cryo-EM structures that capture the process of DNA strand displacement. Our data reveal previously unidentified structural elements that facilitate the unwinding of the downstream DNA duplex. Yeast cells harboring Mip1 variants defective in strand displacement exhibit impaired oxidative phosphorylation and loss of mtDNA, …

Avasimibe Abolishes The Efficacy Of Fluvastatin For The Prevention Of Cancer In A Spontaneous Mouse Model Of Breast Cancer, Anjana Bhardwaj, Alexander Koh, Rhea Bhala, Janvi Sandhu, Zhenlin Ju, Leslie Faye Cando, Jing Wang, Isabelle Bedrosian

Faculty, Staff and Student Publications

The cholesterol biosynthesis pathway is upregulated during breast cancer development and progression. Inhibition of the aberrantly upregulated cholesterol pathway by statins reduces breast tumor incidence and burden by 50% in SV40 C3(1) TAg mice, a mouse model of triple negative breast cancer. We hypothesized that fluvastatin's preventive efficacy could be further enhanced by co-targeting the statin-induced restorative feedback pathways that tightly control the cholesterol pathway and are involved in resistance to statins. Acyl-coenzyme A: cholesterol acyltransferase (

Vdac2 And Bak Scarcity In Liver Mitochondria Enables Targeting Hepatocarcinoma While Sparing Hepatocytes, Shamim Naghdi, Piyush Mishra, Soumya S. Roy, David Weaver, Ludivine Walter, Erika Davies, Anil N. Antony, Xuena Lin, Gisela Moehren, Mark A. Feitelson, Christopher A. Reed, Tullia Lindsten, Craig B. Thompson, Hien T. Dang, Jan B. Hoek, Erik S. Knudsen, György Hajnóczky

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Differences between normal tissues and invading tumors that allow tumor targeting while saving normal tissue are much sought after. Here we show that scarcity of VDAC2, and the consequent lack of Bak recruitment to mitochondria, renders hepatocyte mitochondria resistant to permeabilization by truncated Bid (tBid), a Bcl-2 Homology 3 (BH3)-only, Bcl-2 family protein. Increased VDAC2 and Bak is found in most human liver cancers and mitochondria from tumors and hepatic cancer cell lines exhibit VDAC2- and Bak-dependent tBid sensitivity. Exploring potential therapeutic targeting, we find that combinations of activators of the tBid pathway with inhibitors of the Bcl-2 family proteins …

Flapping Dynamics And Wing Flexibility Enhance Odor Detection In Blue Bottle Flies, Naeem Haider, Zhipeng Lou, Chengyu Li

Faculty Scholarship

One of the most ancient and evolutionarily conserved behaviors in the animal kingdom involves utilizing wind-borne odor plumes to track essential elements such as food, mates, and predators. Insects, particularly flies, demonstrate a remarkable proficiency in this behavior, efficiently processing complex odor information encompassing concentrations, direction, and speed through their olfactory system, thereby facilitating effective odor-guided navigation. Recent years have witnessed substantial research explaining the impact of wing flexibility and kinematics on the aerodynamics and flow field physics governing the flight of insects. However, the relationship between the flow field and olfactory functions remains largely unexplored, presenting an attractive frontier …

Longitudinal Multi-Omics In Alpha-Synuclein Drosophila Model Discriminates Disease- From Age-Associated Pathologies In Parkinson’S Disease, Justin Moore, Timothy Wu, Justin Dhindsa, Omar El Fadel, Anh Le, Alma Perez, Bismark Amoh, Akash Tarkunde, Katy F Zhu, Matthew Avalos, Eric B Dammer, Duc M Duong, Nicholas T Seyfried, Joshua M Shulman, Ismael Al-Ramahi, Juan Botas

Duncan NRI Faculty and Staff Publications

Parkinson's disease (PD) starts decades before symptoms appear, usually in the later decades of life, when age-related changes are occurring. To identify molecular changes early in the disease course and distinguish PD pathologies from aging, we generated Drosophila expressing alpha-synuclein (αSyn) in neurons and performed longitudinal bulk transcriptomics and proteomics on brains at six time points across the lifespan and compared the data to healthy control flies as well as human post-mortem brain datasets. We found that translational and energy metabolism pathways were downregulated in αSyn flies at the earliest timepoints; comparison with the aged control flies suggests that elevated …

Rescue Of Memory, Samuel P. Oliner, Ronnie Swartz

Trade & Scholarly Monographs

What motivates people to help, to care, to risk their own lives for others? What differentiates rescuers, helpers, carers, and bystanders?

There is no single explanation. Anyone who offers you a single explanation of why people do good things is oversimplifying. Empathy is very important. So is courage. And compassion. Bystanders, I want to impress on you, are not evil people; they are not people who should be put in a ship and sent to outer space. They are also good human beings, but they have not internalized some of the ethics of caring, of social responsibility, of knowing. They …

Frequent Disturbance To A Foundation Species Disrupts Consumer-Mediated Nutrient Cycling In Giant Kelp Forests, Joseph R. Peters, Daniel C. Reed, June Shrestha, Scott L. Hamilton, Deron E. Burkepile

Faculty Research, Scholarly, and Creative Activity

Structure-forming foundation species facilitate consumers by providing habitat and refugia. In return, consumers can benefit foundation species by reducing top-down pressures and increasing the supply of nutrients. Consumer-mediated nutrient dynamics (CND) fuel the growth of autotrophic foundation species and generate more habitat for consumers, forming reciprocal feedbacks. Such feedbacks are threatened when foundation species are lost to disturbances, yet testing these interactions requires long-term studies, which are rare. Here, we experimentally evaluated how disturbance to giant kelp, a marine foundation species, affects (1) CND of the forest animal community and (2) nutrient feedbacks that help sustain forest primary production during …

Navigating The Landscape Of Reinterventions After Tavr, Gilbert H L Tang, Syed Zaid, Ole De Backer

Faculty, Staff and Students Publications

No abstract provided.

Zero & Infinity Two Divergent Concepts Converging Into Nothing, Aiden Hills

Osmosis Magazine

The concepts of zero and infinity have perplexed mathematicians, philosophers, and scientists since the very beginning of civilization. These abstract ideas challenged existing paradigms in arithmetic and geometry, paving the way for groundbreaking advances in fields ranging from quantum mechanics to cosmology. Let us examine the resistance and eventual acceptance of zero and infinity, highlighting their indispensable roles in modern science and technology.

Osmosis Magazine - Spring 2024

Osmosis Magazine

Osmosis is a student read, led, and written publication from the University of Richmond, focusing on all aspects of healthcare and science.

Cars Don’T Stop For Yellow Lights: Turning Maine’S Yellow Flag Law Red, Jamison Rhoads-Doyle

Maine Law Review

This Case Note is about Maine’s Extreme Risk Protection Order law, Title 34-B, Section 3862-A, of the Maine Revised Statutes. The purpose of Maine’s law and others like it is to proactively take firearms away from individuals who present a substantial risk of violently harming themselves or others. In the aftermath of the mass shooting which occurred in Lewiston, Maine on October 25, 2023, there was debate over whether law enforcement could have used Section 3862-A to proactively prevent the shooting, as well as over whether Section 3862-A was an effective tool for preventing violence in the first place. This …

Mitigating The Negative Effects Of Over And Early Exposure To Media With Joint Media Engagement: A Literature Review, Mary K. Littlepage

Kentucky Teacher Education Journal: The Journal of the Teacher Education Division of the Kentucky Council for Exceptional Children

This paper examines the negative impact of over and early exposure to media on children’s developmental domains. Among other deleterious effects, early and over exposure to media has been associated with attention deficits, language delays, and interrupted social interactions. The American Academy of Pediatrics (AAP) addresses these concerns with recommendations, discouraging the use of screens for children birth to eighteen months and recommends that children over two years old limit their viewing to one to two hours or less per day (AAP Council on Communications and Media, 2016). For children within the recommended viewing and age parameters, many studies have …

Using Diverse Picture Books To Strengthen Social And Emotional Skills, Jessica L. Edwards

Kentucky Teacher Education Journal: The Journal of the Teacher Education Division of the Kentucky Council for Exceptional Children

Social and emotional learning skills are inherently interconnected with culturally responsive teaching, as any progress with equity and justice requires intrapersonal, interpersonal, and cognitive competence. This article discusses the significance of this connection, providing numerous ideas for using diverse picture books to establish and further strengthen social and emotional learning skills within any classroom environment.