Digital Commons Network^™

Phosphotungstic Acid Staining To Visualize The Vagus Nerve Perineurium Using Micro-Ct, Aniruddha R. Upadhye, Jennifer Coleman, Michael W. Jenkins, David Wilson, Andrew J. Shoffstall

Faculty Scholarship

Background and Purpose: Peripheral nerve stimulation is approved by the US Food and Drug Administration for treating various disorders, but it is often limited by side effects, highlighting the need for a clear understanding of fascicular and fiber organization to design selective therapies. Micro-CT imaging of contrast-stained nerves enables the visualization of tissue microstructures, such as the fascicular perineurium and vasculature. In this work, we evaluated phosphotungstic acid (PTA) as a contrast agent and assessed its compatibility with downstream histology. Methods: Human vagus nerve samples were collected from three embalmed cadavers and subjected to three different staining methods, followed by …

A Computational Framework For Investigating Novel Bacterial Conjugation Inhibitors, Serena Tuytschaevers

Theses

Antimicrobial resistance (AMR) is a major global threat, contributing to an estimated 700,000 deaths annually. One of the main ways that AMR genes spread among bacteria is through conjugation. Therefore, it is crucial to develop conjugation inhibitors (COINs) to combat AMR. Shared characteristics between known COIN molecules are a hydrophobic tail, unsaturations, and a polar head. Considering these characteristics and accounting for the efficacy of 2-hexadecynoic acid and Tanzawaic Acids (TZAs) low toxicity, we designed a new set of TZA analogs. The predicted protein target of previously identified COINs is TrwD, an ATPase in the type IV secretion system (T4SS). …

The Interchangeable-Part Structure Of Food And Drug Law, Adam I. Muchmore

Faculty Scholarship

This Article demonstrates that nested tiers of interchangeable parts serve as the foundation for the regulatory programs administered by the U.S. Food and Drug Administration. Core interchangeable parts used in FDA-administered programs include product categories, prohibitions on adulteration and misbranding, agency-registration requirements, current good manufacturing practices, product standards, marketing authorization requirements, postmarket requirements, and user fees. In each of these areas, Congress or the FDA has engaged in interchangeable-part lawmaking (IPL). IPL occurs when a government takes a portion of its law in one subject area and uses it as a model for its own law in another subject area. …

We’Re All In This Together: A Proposal For Stronger Response To Invasive Plant Species, Marisa Nardelli

Seton Hall Journal of Legislation and Public Policy

No abstract provided.

Montana Kaimin, April 10, 2025, Students Of The University Of Montana, Missoula

Montana Kaimin, 1898-present

Student newspaper of the University of Montana, Missoula.

Allosteric Targeted Drug Delivery For Enhanced Blood-Brain Barrier Penetration Via Mimicking Transmembrane Domain Interactions, Kaicheng Tang, Zhongjie Tang, Miaomiao Niu, Zuyin Kuang, Weiwei Xue, Xinyu Wang, Xinlong Liu, Yang Yu, Seongdong Jeong, Yifan Ma, Annette Wu, Betty Y S Kim, Wen Jiang, Zhaogang Yang, Chong Li

Faculty, Staff and Student Publications

Current strategies for active targeting in the brain are entirely based on the effective interaction of the ligand with the orthosteric sites of specific receptors on the blood-brain barrier (BBB), which is highly susceptible to various pathophysiological factors and limits the efficacy of drug delivery. Here, we propose an allosteric targeted drug delivery strategy that targets classical BBB transmembrane receptors by designing peptide ligands that specifically bind to their transmembrane domains. This strategy prevents competitive interference from endogenous ligands and antibodies by using the insulin receptor and integrin α

A Nuanced Look At How Self-Leadership Skills Influence Academic Success, Ashley Kornya, Patrick J. Cooper

Faculty and Staff Publications & Presentations

No abstract provided.

Spartan Daily, April 10, 2025, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2025

Volume 164, Issue 31

Effects Of Metformin Use On Aneurysmal Subarachnoid Hemorrhage Outcomes, Angel Bueno, Andrea Becerril-Gaitan, Collins Mokua, Kristina Ramirez-Garcia, Justin Nguyen, Faris Shaker, Tien Nguyen, Antonio Dono, Spiros Blackburn, Peng Roc Chen, Mark Dannenbaum, H Alex Choi, Arthur L Day, Jacques J Morcos, Ching-Jen Chen

Faculty, Staff and Student Publications

Background: Metformin is widely prescribed and has neuroprotective effects in animals, but its impact on brain injury after aneurysmal subarachnoid hemorrhage (aSAH) in humans is unclear.

Methods: This single-center retrospective review assessed patients with aSAH from 2009 to 2023, categorizing them based on pre-admission metformin use. The primary outcome was delayed cerebral ischemia (DCI), while secondary outcomes included in-hospital mortality, rebleeding, angiographic cerebral vasospasm (CVS), and favorable modified Rankin Scale (mRS) scores at discharge and the 3-month follow-up. Outcomes were analyzed using logistic regression. Sensitivity analysis was performed after excluding patients receiving comfort care.

Results: A total of 900 patients …

The Zbtb24-Cdca7-Hells Axis Suppresses The Totipotent 2c-Like Reprogramming By Maintaining Dux Methylation And Repression, Dan Guo, Zeling Du, Youqi Liu, Meiqi Lin, Yue Lu, Swanand Hardikar, Yanna Xue, Jinghong Zhang, Taiping Chen, Jiameng Dan

Faculty, Staff and Student Publications

Two-cell-like cells (2CLCs), a rare population (∼0.5%) in mouse embryonic stem cell (mESC) cultures, are in a transient totipotent-like state resembling that of 2C-stage embryos, and their discovery and characterization have greatly facilitated the study of early developmental events, such as zygotic genome activation. However, the molecular determinants governing 2C-like reprogramming remain to be elucidated. Here, we show that ZBTB24, CDCA7, and HELLS, components of a molecular pathway that is involved in the pathogenesis of immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome, function as negative regulators of 2C-like reprogramming by maintaining DNA methylation of the Dux cluster, a master …

The Laureate, Brooklyn Hoover

Honors Theses

The Laureate’s mission is to provide undergraduate students at Western Michigan University a medium through which to publish their fiction, poetry, nonfiction, photography, graphic novels, and other creative works. The Laureate strives to be a professional and engaging journal that appeals to all. The 23^rd edition of The Laureate is unique in that, despite having no strict guidelines on themes, mediums, or styles, we found that many students expressed themes of grief, recovery, self-discovery, and new beginnings. Together, these works create a complex story of introspection and reflection.

Co-editors, Grace Cieslikowski and Brooklyn Hoover facilitated the launch of the …

April 10, 2025, James Madison University

The Breeze, 2020-

The Breeze is the student newspaper of James Madison University in Harrisonburg, Virginia.

Brewers’ Yeast (Saccharomyces Cerevisiae) Purified Functional Feed Additives Mitigate Soybean Meal-Induced Enteritis In Atlantic Salmon (Salmo Salar) Parr, Taofik A. Momoh, Sheu Gbolahan Odu-Onikosi, Folasade Damilola Amulejoye, Joshua Wilson, Benjamin Eynon, Holger Kühlwein, Victor Kuri, Daniel L. Merrifield

School of Biological and Marine Sciences

Soybean meal (SBM) is commonly used in aquafeeds due to its wide availability, reasonable protein content, and cost-effectiveness. However, high SBM inclusion levels in the diets of carnivorous fish, such as Atlantic salmon (Salmo salar), can cause soybean meal-induced enteritis (SBMIE), resulting in compromised gut health, reduced nutrient absorption, and impaired growth. An 8-week study was conducted to evaluate the potential of brewers’ yeast-derived functional feed additives (FFAs), specifically yeast cell wall β-glucans (PβG [purified β-glucan]) and yeast cytosolic extracts (YEs), to mitigate the adverse effects of SBMIE in Atlantic salmon parr. Fish were fed diets containing 30% SBM (30-SBM) …

Sustained Release Of Antifibrotic Nintedanib From Polymer Microparticles Reduces Dosing Frequency While Reducing Inflammation In Murine Idiopathic Pulmonary Fibrosis, Emmanuel Einyat Opolot, Filip Goshevski, Rahul Chaudhary, Horst A. Von Recum, Amar B. Desai

Faculty Scholarship

Purpose: Idiopathic pulmonary fibrosis (IPF) is a life-threatening, progressive lung disease with limited therapeutic options, often resulting in poor patient outcomes. Current treatments, such as Nintedanib (NTB) and Pirfenidone (PFD), require frequent administration, leading to adverse effects and low patient adherence. The purpose of this study was to investigate a sustained-release drug delivery system utilizing microparticles (MPs) composed of insoluble beta-cyclodextrin (β-CD) polymers to enhance the bioavailability and extend the release of NTB and PFD. Methods: A multidisciplinary approach, including in silico modeling, in vitro assays, and in vivo studies, was employed to assess the efficacy of β-CD-polymer MPs as …

Cognitive Correlates Of Borderline Personality Disorder Features In Youth With Bipolar Spectrum Disorders And Bipolar Offspring, Alessio Simonetti, Sherin Kurian, Evelina Bernardi, Antonio Restaino, Francesca Bardi, Claudia Calderoni, Gabriele Sani, Jair C Soares, Kirti Saxena

Faculty, Staff and Student Publications

Background: Bipolar disorder (BD) and borderline personality disorder (BPD) share common cognitive impairments. These deficits are also shared by bipolar offspring (BD-OFF). Nevertheless, little is known regarding the association between cognitive impairments and BPD features in youth with BD and BD-OFF. Objectives: This study aimed to investigate the association between BPD features and cognitive impairments in youth with BD and BD-OFF.

Methods: Thirty-nine participants (7-17 years) with BD, 18 BD-OFF, and 50 healthy controls (HCs) were recruited. BPD features were assessed using the Borderline Personality Features Scale for Children (BPFS-C). Deficits in executive functions and affective processing were assessed using …

Crop Advisor Approaches To Soil Health Advising And Opportunities For Improvement, Peggy Petrzelka, Jessica D. Ulrich-Schad, Matt Yost, Jenae Dean, Earl Creech, Linda Schott, Tony Richards, Kristy Davis

All Current Publications

In this report, we use data from a 2023 online survey of crop advisors in Utah and Idaho to examine how they are working with their clients on soil health and where opportunities for improvement exist.

Jenseits Der Adoption: Wie Kleine Liebesakte Heimtiere Retten Können, Natalie Derewjanko

COD in Lingua

This text explores the positive impact of volunteering at animal shelters and fostering pets. It highlights ways to help animals in need, such as creating homemade toys for cats and offering a loving environment for fostering. The author emphasizes that these small acts of kindness reduce the need for euthanasia, improve animals' chances of adoption, and contribute to a more compassionate society. The text also reflects on the mental well-being benefits for shelter workers and advocates for a kinder, more responsible approach to caring for homeless animals.

Adaptation Of Influenza A Virus Strains A/Anhui/1/2013 (H7n9) And A/Shanghai/1/2013 (H7n9) For Development Of Effective Mouse Models, Spencer Mullen

Student Research Symposium

Introduction

Animal models are key to the research and development of antiviral interventions. Two strains of low-pathogenic avian influenza virus, A/Anhui/1/2013 and A/Shanghai/1/2013 (H7N9), isolated from a 2013 outbreak in China and acquired by USU’s Institute for Antiviral Research from the CDC, are the subject of this project.

Developing antiviral interventions against Influenza A H7N9 is essential because:

• Global outbreaks have caused high mortality among bird and human populations since 2013.^[1]

• Low-pathogenic avian flu viruses can mutate to become highly pathogenic.^[2]

• No current prophylactic or therapeutic human interventions exist.^[3]

BALB/c mice, commonly used for …

Evaluating The Stress System Of The Grieving Brain: Corticotropin-Releasing Factors And Partner Loss In Coyotes, Rachel Tong

Student Research Symposium

Introduction

Social bonds are fundamental and essential

• Positive and negative effects

The stress system¹ :

• Corticotropin-releasing factor (CRF) system
• CRFR1 and CRFR2
• Acts on the HPA axis, amygdala, and hippocampus

Genetic monogamy is common among coyotes

Importance:

• Coyotes’ greatest threat are humans
• An abandoned partner can disrupt biology and behavior

Animals experience social loss too!

Media Entertainment, Louis Bosshart, Ilaria Macconi

Communication Research Trends

No abstract provided.

Biosecurity Strategies For Combating Iuu Fishing: Protecting Ecosystems And Economies, Spencer Anderson

Student Research Symposium

Illegal, Unreported, and Unregulated (IUU) fishing threatens marine ecosystems and global economies by driving overfishing, spreading aquatic diseases, and introducing invasive species. It disrupts biodiversity, jeopardizes fisheries' sustainability, and undermines the livelihoods of millions, intensifying food insecurity and socio- economic vulnerabilities, particularly in fishing- dependent communities.

This research examines biosecurity strategies— including regulatory frameworks and collaborative efforts—to safeguard marine biodiversity and support sustainable fishing economies. Addressing the complex challenges of IUU fishing bridges gaps between conservation, public health, and economic resilience.

This study adopts an interdisciplinary approach to develop practical solutions to preserve biodiversity, enhance public health protections, and promote …

Environmental Racism: The Role Of Racism In The Unequal Distribution Of Environmental Hazards And Resources In Urban Areas, Acadia Petersen

Student Research Symposium

Introduction

There is a large difference in the number of environmental hazards and environmental resources that people live with depending on their race. This is very damaging as certain minorities are being provided with fewer resources and more polluted areas, leading to worse health outcomes.

There is “A strong association between non-white and poor communities and the location of hazardous waste sites…Studies on air quality have documented that minorities and the poor face higher levels of air pollution than other groups” (Pinderhughes 1996).

Martha Stewart's Enduring Legacy: A Social Media Case Study, Holly Asay

Student Research Symposium

Martha Stewart gained fame in the 1990s and, at 83 years old, remained culturally relevant on her personal Instagram account. Despite early criticism, she had 2.6M followers and high engagement in 2025.

Establishing Methods For In Vitro Static Culture Of Gut Microbiomes For Nutritional Intervention Studies, Jeremy Case

Student Research Symposium

The National Cancer Institute reports colorectal cancer as the leading cause of cancer death for individuals under 50 in the United States (American Cancer Society). These rates continue to rise with poor diet, microbiome dysbiosis, and gut inflammation being the primary drivers.

Our lab performs nutrient- intervention studies on DSS/AOM colitis-induced mouse models fed the total Western diet, reflective of the average American’s diet. A poor diet promotes gut microbiome dysbiosis and subsequent colitis, ultimately yielding colon cancer. We seek to understand how the introduction of a bioactive compound may lessen or promote these effects in in vivo mouse models. …

Kosher Kitchens: A Balance Of Faith And Function, Kirra Mcentire

Student Research Symposium

Jewish dietary regulations (kashrut) are followed as a religious practice derived from the Torah, teaching what food one can eat and how to prepare it. The customs require the separation of meat and dairy, the consumption only of specific beasts, and the adherence of ritual slaughter (shechita). Being kosher is a way in which Jewish individuals can be spiritually trained and obey God's commandments while generating a sense of sanctity within daily life.

Aside from religious significance, keeping kosher also emphasizes ethical eating and cultural heritage. The practice promotes humane treatment of animals, cleanliness in food handling, and enriching Jewish …

Farmstead Therapy: Intergenerational Connection And Sense Of Place Via Equine Therapy, Kaleb Griffeth

Student Research Symposium

Therapy has become a crucial element within society. It has offered relief, understanding, and hope for a changed future to hundreds of millions of people just in the last five years (SAMHSA, 2004).

On another front, division between generations seems to be growing by the month. Political, social, and economic differences between generations “X”, “Z”, Alpha, and Baby Boomers make it appear that there is no connection whatsoever across age gaps.

Because equine therapy has proven a succesful branch, especially for those of a younger demographic, Farmstead Therapy was a project designed to bring together the skills of the older …

Anthropological Perspectives Of The Relationship Between Landscape And Human Health In The Neo-Assyrian Empire, Sydnee Jensen

Student Research Symposium

Drought can directly impact human health through agriculture. In the distant past, human health data is limited, however we can use studying agricultural data to gain insight into the impacts of drought on human health. This study focuses on phytoliths from animal diets in to shed light on human health during the Assyrian Empire (911 – 609 BCE), an agrarian empire that declined during an arid period.

Exercise And Its Effect On The Nad Content Of Tissues In Niacin Deficient Mice With Free Choice Running Wheels, Stellaluna Star

Student Research Symposium

Nicotinamide adenine dinucleotide (NAD) content of tissues decreases over time during aging. In our study, we determine if physical exercise has an effect on the NAD content in tissues of transgenic mice that were NAD- deficient because of vitamin B3-free diet. The underlying hypothesis of this study is that exercise can prevent NAD decline and maintain higher level of NAD in tissue. A potential impact of this study is that exercise could be used to lengthen healthspan compared to individuals not participating in any physical exercise.

Grassland For Our World, F Riveros

IGC Proceedings (1977-2023)

The present-day role of grasslands and fodder is reviewed with respect agriculture, animal production and environmental concerns. The world 's grazing lands are a finite resource nnd, overall, are being badly mnnaEed; in most developing countries action is required now, before it is too lnte. They will continue to be a major source of animal feed for the foreseeable future; encroachment from cropping will con

Effect Of Irrigation On Vegetation Succession And Forage Regerneration, Fu Jing-Yan

IGC Proceedings (1977-2023)

No abstract provided.