Digital Commons Network^™

The Bullied Prairie Vole: Modeling Depression And Anxiety Through Social Defeat Stress, Minerva Rodriguez

Open Access Theses & Dissertations

Stress-induced illnesses, such as major depression and anxiety, are significant global causes of disability. This highlights the need for reliable animal models that accurately mimic social stress-induced maladaptations, thus, allowing the experimental study of the etiology of such disorders. Prairie voles (Microtus ochrogaster), known for their human-like bi-parental behavior and monogamous partner preferences, are a relevant model for the study of stress-induced psychopathologies. For this reason, in this dissertation, I examined whether exposing voles to the social defeat stress (SDS) paradigm, an experimental approach that captures bullying behavior, would result in both depression and anxiety-related profiles. To do this, experimental …

Regulation Of Ccr7 Signaling Events By G-Protein-Coupled Receptor Kinases 2 And 3, Anahi Sanchez

Open Access Theses & Dissertations

G protein-coupled receptors (GPCR) can bind multiple ligands, leading to differential signaling events termed biased signaling. C-C chemokine receptor 7 (CCR7) is a GPCR known to have biased signaling between the two ligands it binds to, CCL19 and CCL21. Binding of C-C Chemokine Ligand 19 (CCL19) promotes a high level of CCR7 phosphorylation, while CCL21 leads to a much lower level of CCR7 phosphorylation; findings observed in HEK and dendritic cells. GPCR kinases (GRKs), which can control the phosphorylation patterns of a GPCR, are hypothesized to regulate biased signaling of a receptor. GRK expression differs between cell lines and cell …

Lizita, Meet Liz A Memoir, Elizabeth Shannon

Open Access Theses & Dissertations

A change of life baby, Liz was born to poor, middle-aged Mexican American parents who didn't want the same things in life that she wanted. Generational trauma, internalized racism, and family dysfunction permeated her psyche and crippled her self-worth sending her into the fantasy worlds she created in her head. She managed to escape her family through marriage at nineteen years old, quickly realizing she had traded family dysfunction for marital dysfunction. But, then, an affair with a man more than twenty years her junior forced her to face the reality of her marriage. Newly divorced and finally free to …

Efecto De Soles, Natalia Soriano Moreno

Open Access Theses & Dissertations

En este texto quiero exponer cómo fue el proceso de creación de Efecto de soles, el cual se desarrolló entre los años 2022 y 2025. El libro presenta la cotidianidad de un barrio que un día se ve afectado por distintos cambios en la naturaleza cuando aparece un fenómeno particular: los soles de algunas casas se vuelven cuadrados y aumentan su nivel de alumbramiento. Esto hace que el entorno natural del barrio comience a cambiar: aparecen gusanos fosforescentes, las flores pierden su olor, la lluvia se vuelve negra, las mariposas se van y a los árboles les salen hojas de …

Women’S Participation In Homegrown And Domestic Terrorism Plots, Caroline Johnson

Graduate Theses and Dissertations

The 21^st century ushered in major social, political, and cultural changes in the United States, including a new era of homeland security and new forms of domestic terrorism. Little is known about how such shifts may have impacted women’s participation in terrorism plots. In response, data from the American Terrorism Study (ATS) are used to comparatively examine the involvement of women in homegrown and domestic terrorism plots before and after the 9/11 terrorist attacks. Findings of similarities and differences in women’s involvement in terrorism across time periods are presented and contextualized.

Ghrelin And Decision-Making: Exploring Its Role In Food Reward, Novelty-Seeking, And Cost Valuation, Serina A. Batson

Open Access Theses & Dissertations

When low energy levels are detected, Ghrelin, a peptide hormone, is produced in the fundus of the stomach. Referred to as the "hunger hormone", Ghrelin is traditionally associated with increased food intake and blood sugar levels. A key modulator in the gut-brain interaction, Ghrelin's growth hormone secretagogue receptor can be found widely across the cortico-striosomal circuit. Critical for decision-making, Ghrelin-based manipulation of this circuit can directly affect reward and cost valuation. While Ghrelin is uniformly considered to increase the valuation of food-based rewards, little research has explored its impact on non-food-based rewards or its effects on cost valuation. Therefore, to …

The Feast And The Furious? The Effects Of Diet On Opioid Tolerance, Withdrawal, And Stress, Nina Marie Beltran

Open Access Theses & Dissertations

Introduction: Opioid drugs (e.g., morphine) are used medicinally for pain relief but can also lead to opioid use disorder (OUD). These medications are prescribed at a higher rate among individuals with obesity (who might consume high fat diets) than the general population. Further, previous work from our lab and others has demonstrated that eating a high fat diet enhances sensitivity of rats to other addictive substances, including stimulants. However, it is not known if dietary manipulations impact individual sensitivity to the effects of opioids. This dissertation systematically explored the effects of dietary manipulation on sensitivity to the effects of morphine. …

The Depths Of Horror: Exploring The Manifestation Of Fear In The Natural World, Jordan Eugene Easley

Open Access Theses & Dissertations

The horror genre is uniquely powerful in that it is one of the only hands of fiction with the ability to reach off the page and follow you. The feeling of fear originally formed in the confines of a scary story can lie dormant in the reader after the story has finished and be resurfaced later by a related trigger. On a late night after work walking through a deserted car garage, home alone in the middle of a storm, or stumbling into an abandoned hospital, the reader is imbued with an ability to detect a threat that was previously …

Testing A Benign Multi-Modal Bilingual Intervention To Increase Self-Efficacy, Intentions, And Willingness To Administer A Covid-19 Antigen Rapid Test In A Predominately Hispanic Sample, Uchenna Gbugu

Open Access Theses & Dissertations

Introduction. Testing is one of the most effective methods for reducing the spread of COVID-19. COVID-19 antigen tests can detect proteins from the COVID-19 virus called antigens and produce results within minutes, making them suitable for at-home use. Several factors have been identified as barriers to self-administering a COVID-19 antigen test, including the language of the testing instructions, the cost of testing, and access to tests. Interventions to reduce barriers to self-administering COVID-19 rapid tests are warranted to reduce the spread of COVID-19. The purpose of the present study is to test the impact of a benign multi-modal bilingual intervention …

Elevated P16ink4a Expression Enhances Tau Phosphorylation In Neurons Differentiated From Human-Induced Pluripotent Stem Cells, Kristopher Holloway, Kashfia Neherin, Yingduo Song, Kazuhito Sato, Andrew Houston, Feng Chen, Li Ding, Hong Zhang

2020-Current year OA Pubs

Increased expression of the cyclin-dependent kinase inhibitor p16Ink4a (p16) is detected in neurons of human Alzheimer's disease (AD) brains and during normal aging. Importantly, selective eliminating p16-expressing cells in AD mouse models attenuates tau pathologies and improves cognition. But whether and how p16 contributes to AD pathogenesis remains unclear. To address this question, we tested whether induction of p16 expression in neurons exacerbates AD pathologies. We created a doxycycline-inducible system to trigger p16 up-regulation in human-induced pluripotent stem cells (iPSCs) and neurons differentiated from iPSCs. We demonstrated that up-regulated p16 expression in iPSCs reduces cell proliferation, down-regulates cell cycle genes, …

American Academy Of Dermatology Acne Guidelines Of Care Versus Modern Skincare Trends, Natasha Barton, Patrick Mcclain, Monika Ziogaite, Robert P. Dellavalle, Cory A. Dunnick

Student Publications

Acne vulgaris remains one of the most prevalent dermatologic conditions worldwide, yet its management has become increasingly influenced by pop culture trends and consumer marketing. This review critically compares the American Academy of Dermatology (AAD) guidelines for acne treatment with the most popular skincare practices of the 2020s, including natural remedies, light therapy, and multi-step routines popularized through platforms like TikTok and Instagram. By examining the scientific evidence supporting AAD-recommended treatments, such as topical retinoids, benzoyl peroxide, and systemic therapies, and contrasting them with trending regimens that often lack clinical validation, this paper explores the widening gap between expert guidance …

Computational And Functional Prioritization Identifies Genes That Rescue Behavior And Reduce Tau Protein In Fly And Human Cell Models Of Alzheimer Disease, Morgan C Stephens, Jiayang Li, Megan Mair, Justin Moore, Katy Zhu, Akash Tarkunde, Bismark Amoh, Alma M Perez, Arya Bhakare, Fangfei Guo, Joshua M Shulman, Ismael Al-Ramahi, Juan Botas

Faculty, Staff and Students Publications

Genome-wide association studies (GWASs) in Alzheimer disease (AD) have uncovered over 70 loci significantly associated with AD risk, but identifying the true causal gene(s) at these loci requires systematic functional validation that is rarely performed due to limitations of time and cost. Here, we integrate transcriptome-wide association study (TWAS) with colocalization analysis, fine-mapping, and additional annotation of AD GWAS variants to identify 123 genes at known and suggestive AD risk loci. A comparison with human AD brain transcriptome data confirmed that many of these candidate genes are dysregulated in human AD and correlate with neuropathology. We then tested all available …

Glands Of Moll: History, Current Knowledge And Their Role In Ocular Surface Homeostasis And Disease, Michael Stopfer, Ingrid Zahn, Katharina Jüngert, Gerhard Aumüller, Frans L Moll, Martin Schicht, Helen P Makarenkova, Cintia S De Paiva, Friedrich P Paulsen

Faculty, Staff and Students Publications

Over the last 20 years, research into the Meibomian glands of the eyelids has increased exponentially and is now widely recognized as a field of research. It is all the more astonishing that knowledge about another type of gland in the eyelids, the Moll glands or ciliary glands, has almost stagnated and there has been little to almost no progress, even though this type of gland as a whole takes up a relatively large volume in the upper and lower eyelids. There is not much information about the namesake Moll or the function of the glands although these are listed …

Cross-Tissue Coordination Between Slc Nucleoside Transporters Regulates Reproduction In Caenorhabditis Elegans, Youchen Guan, Yong Yu, Shihong M Gao, Lang Ding, Qian Zhao, Meng C Wang

Faculty, Staff and Students Publications

Metabolism is fundamental to organism physiology and pathology. From the intricate network of metabolic reactions, diverse chemical molecules, collectively termed metabolites, are produced. In multicellular organisms, metabolite communication between different tissues is vital for maintaining homeostasis and adaptation. However, the molecular mechanisms mediating these metabolite communications remain poorly understood. Here, we focus on nucleosides and nucleotides, essential metabolites involved in multiple cellular processes, and report the pivotal role of the SLC29A family of transporters in mediating nucleoside coordination between the soma and the germline. Through genetic analysis, we discovered that two Caenorhabditis elegans homologs of SLC29A transporters, Equilibrative Nucleoside Transporter …

Locked Dimerized Cxcl12 Exerts Radiosensitizing Effects In Head And Neck Cancer, Oscar Villarreal Espinosa, Musaddiq Awan, Abdullah A. Memon, Anne Frei, Jamie Foeckler, Rachel Kuehn, Jennifer Bruening, Becky Massey, Stuart J. Wong, Monica Shukla, Julia Kasprzak, Amit Joshi, Michael B. Dwinell, Heather A. Himburg, Joseph Zenga

Biomedical Engineering Faculty Research and Publications

Background

Head and neck squamous cell carcinoma (HNSCC) presents significant treatment challenges, particularly in cases unrelated to human papillomavirus (HPV). The chemokine receptor CXCR4, interacting with its ligand CXCL12, plays a crucial role in tumor proliferation, metastasis, and treatment resistance. This study explores the therapeutic potential of engineered monomeric and dimerized CXCL12 variants (CXCL121 and CXCL122, respectively) in HNSCC and evaluates potential additive effects when combined with radiation therapy.

Methods

Clinical HNSCC biopsies were evaluated for CXCR4 expression in both previously untreated and radiorecurrent disease. HNSCC cell lines were then treated with combinations of CXCL12 variants and radiotherapy and interrogated …

Involvement Of Long Non-Coding Rna (Lncrna) Malat1 In Shear Stress Regulated Adipocyte Differentiation, Justin Caron, Marjan Ghanbariabdolmaleki, Madison Marino, Chong Qiu, Bo Wang, Michael Mak, Shue Wang

Biomedical Engineering Faculty Research and Publications

Adipocyte differentiation plays an important role in bone remodeling due to secretory factors that can directly modulate osteoblast and osteoclast, thus affecting overall bone mass and skeletal integrity. Excessive adipocyte differentiation within the bone marrow microenvironment can lead to decreased bone mass, eventually causing osteoporosis. The mechanical microenvironment of bone marrow, including fluid shear, maintains the balance of adipocyte and osteoblast differentiation during bone remodeling. However, how mechanical cues interact with long noncoding RNA (lncRNA) and regulate adipocyte differentiation remains unexplored. In this study, we investigated the mechanosensitive role of lncRNA MALAT1 during mesenchymal stem cells (MSCs) adipocyte differentiation. By …

Introducing Social Determinants Of Health To The Alzheimer's Disease Research Center Network: Development And Implementation In The Uniform Data Set, Megan Zuelsdorff, Erin L. Abner, Joyce E. Balls-Berry, Gregory A. Jicha, Serggio Lanata, Gladys Maestre, Monica Rosselli, Shana D. Stites, Rachel A. Whitmer, Consuelo H. Wilkins

School of Medicine Publications

The Alzheimer's Disease Research Centers (ADRCs) consortium represents a critical locus of research on Alzheimer's disease and related disorders (ADRD) prevention, diagnosis, and intervention. Through the National Alzheimer's Coordinating Center's (NACC) standardized protocol, the Uniform Data Set (UDS), ADRCs have collected rich, harmonizable clinical and cognitive data. However, the collection of social data has been sparse and Center specific, constraining ADRD science that addresses research priorities on social determinants of health (SDOH) and health equity. Capitalizing on the transition to a revised UDS version 4, an interdisciplinary committee representing 10 ADRCs reviewed the literature and instrumentation, ultimately creating a brief …

Promoting Global Collaboration To Improve Bioaerosol Exposure Assessment And Understanding Of Associated Health Impacts: Outcomes From A Series Of Workshops, Emma L. Marczylo, Simon Jackson, Christine Bell, Daniel Andrews, Martin J.D. Clift, Ian Crawford, Gyorgy Fejer, Robert M.W. Ferguson, Matthew C. Fisher, Emma Jane Goode, James Isaac, Rob Kinnersley, Julie A. Morrissey, Sofya Pozdniakova, Carla Viegas, Andrew Ward, Inge M. Wouters, Frederic Coulon, Zaheer A. Nasir, Philippa Douglas

School of Biomedical Sciences

We are surrounded, in both indoor and outdoor environments, by air containing particles of biological origin (bioaerosols). We constantly inhale them, and, depending upon their size, they deposit in different parts of our airways. Despite their ubiquitous nature and our constant exposure, bioaerosol diversity and composition of the environment are not well characterized, and we understand little about which bioaerosols we are exposed to and how this impacts our health, either positively or negatively. Indoor/Outdoor Bioaerosols Interface and Relationships Network (BioAirNet), a Clean Air Programme-funded network, has recognized the need for the bioaerosol community to reflect on the current challenges …

A Standardized Nomenclature For The Rods And Cones Of The Vertebrate Retina, Tom Baden, Takeshi Yoshimatsu, Joseph C. Corbo, Et Al.

2020-Current year OA Pubs

Vertebrate photoreceptors have been studied for well over a century, but a fixed nomenclature for referring to orthologous cell types across diverse species has been lacking. Instead, photoreceptors have been variably-and often confusingly-named according to morphology, presence/absence of 'rhodopsin', spectral sensitivity, chromophore usage, and/or the gene family of the opsin(s) they express. Here, we propose a unified nomenclature for vertebrate rods and cones that aligns with the naming systems of other retinal cell classes and that is based on the photoreceptor type's putative evolutionary history. This classification is informed by the functional, anatomical, developmental, and molecular identities of the neuron …

The Multiverse Of Cd46 And Oncologic Interactions, M. Kathryn Liszewski, John P. Atkinson

2020-Current year OA Pubs

Initially identified as a regulator of complement activation on host cells, the known roles of CD46 (membrane cofactor protein [MCP]) have expanded. We now know that this ancient molecule is expressed on almost all nucleated cells as a family of four predominant isoforms. It also is involved in human reproduction, modulation of T cell activation and immunoinflammatory effector functions, autophagy, and the newly identified intracellular complement system (complosome). CD46 is also known as a "pathogen" magnet, being a port of entry for at least seven bacteria and five viruses. Moreover, CD46 has recently emerged as a key player in cancer …

Multi-Omic And Spatial Analysis Of Mouse Kidneys Highlights Sex-Specific Differences In Gene Regulation Across The Lifespan, Siqi Chen, Ruiyang Liu, Chia-Kuei Mo, Michael C Wendl, Andrew Houston, Preet Lal, Yanyan Zhao, Wagma Caravan, Andrew T Shinkle, Atieh Abedin-Do, Nataly Naser Al Deen, Kazuhito Sato, Xiang Li, André Luiz N Targino Da Costa, Yize Li, Alla Karpova, John M Herndon, Maxim N Artyomov, Joshua B Rubin, Sanjay Jain, Sheila A Stewart, Li Ding, Feng Chen, Et Al.

2020-Current year OA Pubs

There is a sex bias in the incidence and progression of many kidney diseases. To better understand such sexual dimorphism, we integrated data from six platforms, characterizing 76 kidney samples from 68 mice at six developmental and adult time points, creating a molecular atlas of the mouse kidney across the lifespan for both sexes. We show that proximal tubules have the most sex-biased differentially expressed genes emerging after 3 weeks of age and are associated with hormonal regulations. We reveal potential mechanisms involving both direct and indirect regulation by androgens and estrogens. Spatial profiling identifies distinct sex-biased spatial patterns in …

Multi-Epitope Immunocapture Of Huntingtin Reveals Striatum-Selective Molecular Signatures, Joshua L Justice, Todd M Greco, Josiah E Hutton, Tavis J Reed, Megan L Mair, Juan Botas, Ileana M Cristea

Faculty, Staff and Students Publications

Huntington's disease (HD) is a debilitating neurodegenerative disorder affecting an individual's cognitive and motor abilities. HD is caused by a mutation in the huntingtin gene producing a toxic polyglutamine-expanded protein (mHTT) and leading to degeneration in the striatum and cortex. Yet, the molecular signatures that underlie tissue-specific vulnerabilities remain unclear. Here, we investigate this aspect by leveraging multi-epitope protein interaction assays, subcellular fractionation, thermal proteome profiling, and genetic modifier assays. The use of human cell, mouse, and fly models afforded capture of distinct subcellular pools of epitope-enriched and tissue-dependent interactions linked to dysregulated cellular pathways and disease relevance. We established …

Restating The Law Of Children And Youth: The Evolution Of Reform, Elizabeth S. Scott

Faculty Scholarship

A brief roadmap of this article may be helpful. Part I describes the emergence of the Child Wellbeing framework and its powerful role in shaping twenty-first-century youth justice reforms captured by the Restatement. Part II shows how this framework has begun to have a far broader influence on the law affecting children and how the framework and the developmental approach embodied in the Restatement can guide courts and shape the trajectory of the law in the future. Part III explores potential challenges to the Restatement’s effectiveness in attaining a fully integrated system based on the Child Wellbeing framework.

Angelic Wasting: The Impacts Of The Enlightenment, Evangelicalism, And The Industrial Revolution On Romanticization Of Tuberculosis In England, 1800-1850, Sara M. Sudano

Electronic Theses and Dissertations

This thesis aims to elucidate the intersections between the portrayal of tuberculosis in the Romantic era and the Enlightenment, evangelism, and the Industrial Revolution. This study aims to do so by locating and analyzing depictions in the primary source representations of the disease, then considering the effects of the three factors mentioned above on its representation. The Enlightenment, evangelism, and the Industrial Revolution interacted in a variety of ways, and the influence of all three factors was found within the representations of tuberculosis, albeit in often indirect ways. In the novels of Elizabeth Cleghorn Gaskell and Charlotte Brontë, tuberculosis was …

The Scream Notation System: A Notational System For Screamed Vocals, Matthew Delcambre

Electronic Theses and Dissertations

In Western music notation, there is no specified system of notation for screamed vocals, a style of singing that has become increasingly popular since the late 1970s with the advent of metal music and seen increased interest in screams in other genres since. Screams are a unique and diverse vehicle of delivery that can be differentiated according to timbre, technique, pitch and physiology. This research aims to first identify the anatomical and timbral differences between named screams and create a database of screams to be studied. The next task is to design a notational system appropriate for screams and then …

Initial Impacts Of Different Fertilizers On Improved East Texas Equine Pastures, Carolina De Leon, Brian Oswald, Leland Thompson, Candis Scallan

Electronic Theses and Dissertations

ABSTRACT

In Texas, approximately 1.6 million acres support an equine population exceeding 748,000 horses. This study assessed the effects of organic and inorganic fertilizer treatments on improved Bermudagrass (BG) and other vegetation (OTH) in overgrazed equine pastures at the Stephen F. Austin State University Equine Center. Treatments included equine manure (H), broiler poultry litter (P), and a commercial 13-13-13 fertilizer (C), with application rates based on pasture requirements.

Initial vegetation cover was evaluated using the Daubenmire cover class method in September 2024, followed by a 60-day undisturbed growing period. Final biomass (g/2ft² converted to lbs/ac) and percent cover measurements were …

Per- And Polyfluoroalkyl Substances In A Suburbanizing Watershed, Bailey Mullins

Master's Theses and Capstones

Per- and polyfluoroalkyl substances (PFAS) are persistent man-made chemicals that accumulate in aquatic environments due to their widespread industrial and commercial use and resistance to degradation. Understanding their spatial distribution and relative abundance in streams draining suburbanizing watersheds is crucial for effective watershed and water quality management. This study investigates how the concentrations and composition of PFAS in surface water and sediment are impacted by urbanization. PFAS were quantified in the Lamprey River watershed, a coastal watershed in southeastern New Hampshire that is experiencing ongoing suburbanization. Sampling sites include tributaries, mainstem, and estuarine areas. Surface water, sediment, and well water …

Polyphenol-Protein Interactions In Pigmented Cereals – A Clean-Label Approach To Tailor Starch Viscosity, Annegret Jannasch

Graduate Theses and Dissertations

Viscosity plays an important role in the food industry as it affects the quality, processing stability, and shelf-life of food and impacts physiological responses such as satiation and satiety. Greater viscosity reduces food intake by increasing the perception of satiety, primarily by delaying gastric emptying and increasing tactile stimulation. Starch is commonly added to food products as a viscosifying ingredient, which often involves chemically modifying the starch via cross-linking, to enhance its resistance to high shear, temperature and acidity. However, chemically modified starches are not “clean-label” ingredients due to the use of toxic chemicals. Therefore, further exploration is needed to …

Identifying Microbial Complexities And Control Strategies At Pre-Harvest Poultry Production, Tolulope Tiwa Ogundipe

Graduate Theses and Dissertations

While significant progress has been made in post-harvest control of Salmonella in the poultry industry, the development of effective pre-harvest intervention strategies remains critical for reducing foodborne pathogens. Environmental reservoirs such as poultry litter and drinking water systems (DWS) are potential harborage sites for Salmonella and other microbial communities. Understanding the microbiota of these environments, including biofilms within water lines, is essential to identifying factors contributing to Salmonella colonization and improving pre-harvest control approaches. This study aimed to evaluate the incidence and quantity of Salmonella in poultry litter and water lines, characterize the microbial populations in these environments, and assess …

In Vitro Investigation Of Macrophage Functional And Metabolic Modulation, Shelby Nicole Bess

Graduate Theses and Dissertations

Macrophages are a subset of immune cells with a variety of functions that are abundant in numerous tumor types such as colorectal and breast cancer. It has been suggested that macrophages may serve an important function as immune mediators in tumor behavior; however, macrophage activity is complex and remains not completely understood. The overall goal of this dissertation was to explore and exploit tumor-associated macrophage phagocytic function and the changes in their interactions with cancer cells in an in vitro mouse model and in 2D and 3D in vitro culture models. First, we wanted to better understand macrophage behavior under …