Digital Commons Network^™

Trem2 Depletion In Pancreatic Cancer Elicits Pathogenic Inflammation And Accelerates Tumor Progression Via Enriching Il-1Β+ Macrophages, Daowei Yang, Xinlei Sun, Hua Wang, Ignacio I Wistuba, Huamin Wang, Anirban Maitra, Yang Chen

Faculty, Staff and Student Publications

Background & aims: Pancreatic ductal adenocarcinoma (PDAC) has a complex tumor microenvironment enriched with tumor-associated macrophages. Triggering receptor expressed on myeloid cells 2 (TREM2) is highly expressed by a subset of macrophages in PDAC. However, the functional role of TREM2 in PDAC progression remains elusive.

Methods: We generated a novel transgenic mouse model (KPPC;Trem2-/-) that enables the genetic depletion of TREM2 in the context of spontaneous PDAC development. Single-cell RNA-sequencing analysis was used to identify changes in the tumor immune microenvironment on TREM2 depletion. We evaluated the impacts of TREM2 depletion on the tumor immune microenvironment to elucidate the functions …

Gsk-3484862, A Dnmt1 Degrader, Promotes Dnmt3b Expression In Lung Cancer Cells, Qin Chen, Swanand Hardikar, Kimie Kondo, Nan Dai, Ivan R Corrêa Jr, Meigen Yu, Marcos R Estecio, Xing Zhang, Taiping Chen, Xiaodong Cheng

Faculty, Staff and Student Publications

DNA methylation alterations, including hypermethylation and silencing of tumor suppressor genes, contribute to cancer formation and progression. The FDA-approved nucleoside analogs azacytidine and decitabine are effective demethylating agents for hematologic malignancies but their general use has been limited by their toxicity and ineffectiveness against solid tumors. GSK-3484862, a dicyanopyridine-containing, DNMT1-selective inhibitor and degrader, offers a promising lead for developing novel demethylating therapeutics. Here, we demonstrate that GSK-3484862 treatment upregulates DNMT3B expression in lung cancer cell lines (A549 and NCI-H1299). Disrupting DNMT3B in NCI-H1299 sensitizes these cells to GSK-3484862, enhancing its inhibitory effects on cell viability and growth. GSK-3484862 treatment induces …

Nlrp3 Inflammasome Activation Expands The Immunosuppressive Myeloid Stroma And Antagonizes The Therapeutic Benefit Of Sting Activation In Glioblastoma, Spencer T Lea, Chao-Hsien Chen, Jun Wei, Ivana William, Inés Lopez Del Castillo, Michael A Curran

Faculty, Staff and Student Publications

Glioblastoma (GBM) is the most common and deadly primary brain malignancy and is clinically refractory to immunotherapy. Active NLRP3 inflammasome signaling and IL-1β secretion have been observed in GBM, and NLRP3-driven myeloid-derived suppressor cell (MDSC) recruitment can mediate cancer immune evasion. Agonists of the cytosolic double-stranded DNA-sensing stimulator of IFN gene (STING) pathway can mediate proinflammatory conversion of cancer MDSCs; however, secretion of the NLRP3 products IL-1β and IL-18 has also been observed in certain myeloid populations following STING activation. In this study, we aimed to determine both the potential mechanistic synergy between STING and NLRP3 agonists, and the effects …

A Developmental Texture Framework For Food Texture Progression: Implications For Feeding Development, Oral Motor Skills, And Pediatric Feeding Disorder, Amy L. Delaney, Sarah Staskiewicz, Suzanne Evans Morris, Sarah Smith-Simpson, Praveen S. Goday

Speech Pathology and Audiology Faculty Research and Publications

The introduction of food textures in a child's first 2 years of life plays a vital role in growth, nutrition, and feeding development. However, the absence of a standardized texture framework for studying texture progression limits both pediatric feeding research and the ability to diagnose pediatric feeding disorder (PFD) based on age-appropriate expectations. To address this gap, authors Delaney and Goday proposed data sharing from the Nestlé Feeding Infant-Toddler Study (FITS) to explore texture progression and define age-appropriate texture expectations. In response, Nestlé assembled a multidisciplinary panel of feeding experts to create standardized textures but did not provide financial or …

Roadmap For Mainstreaming Integrated Pest Management (Ipm) Into A Climate Smart One-Health (Cs-Oh) Framework, Henri E.Z. Tonnang, Ghislain T. Tepa-Yotto, Bonoukpoè Mawuko Sokame, Jeannette K. Winsou, Manuele Tamò, Rousseau F. Djouaka

All Peer-Reviewed Publications

Climate change presents significant challenges to agricultural sustainability, particularly in integrated pest management (IPM). To address these challenges, we propose a holistic and interdisciplinary conceptual framework, climate-smart One-Health (CS-OH), which integrates ecological, environmental, and socio-economic factors, considering human, animal, soil, and water health within the environment. This paper introduces a roadmap for Climate-Smart OH IPM, combining One-Health (OH) principles with climate-smart agriculture to promote sustainable pest management amid climate change. The roadmap utilizes Systems Thinking (ST) & System Dynamics (SD) methodologies to comprehend complex interactions in climate-affected agricultural systems. Additionally, we provide a step-by-step implementation of Digital Twin (DT) IPM, …

Status And Distribution Of Diseases Caused By Phytoplasmas In Africa, Shakiru Adewale Kazeem, Agnieszka Zwolińska, Joseph Mulema, Akindele Oluwole Ogunfunmilayo, Shina Salihu, Joy Oluchi Nwogwugwu, Inusa Jacob Ajene, Justina Folasayo Ogunsola, Adedapo Olutola Adediji, Olubusola Fehintola Oduwaye, Kouamé Daniel Kra, Mustafa Ojonuba Jibrin, Wei Wei

All Peer-Reviewed Publications

Phytoplasma (“Candidatus Phytoplasma” species) diseases have been reported globally to severely limit the productivity of a wide range of economically important crops and wild plants causing different yellows-type diseases. With new molecular detection techniques, several unknown and known diseases with uncertain etiologies or attributed to other pathogens have been identified as being caused by Phytoplasmas. In Africa, Phytoplasmas have been reported in association with diseases in a broad range of host plant species. However, the few reports of Phytoplasma occurrence in Africa have not been collated together to determine the status in different countries of the continent. Thus, this paper …

One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika

All Peer-Reviewed Publications

The global human population is rapidly increasing, escalating interactions of people, animals and the environment. This has led to more convoluted health challenges, for which African smallholder farmers bear the brunt. The One Health (OH) approach recognises the interconnectedness of these health challenges and thus follows a transdisciplinary approach involving diverse stakeholders to address them. Although there have recently been global concerted efforts and studies on OH, an information gap exists in Africa, particularly regarding smallholder farmers and their needs. Additionally, evaluation frameworks and outcome metrics for OH initiatives are still unclear. Thus, this study was conducted to critically map …

Blockade Of A2ar Improved Brain Perfusion And Cognitive Function In A Mouse Model Of Alzheimer’S Disease, Mariana Sayuri Berto Udo, Julia Zaccarelli-Magalhães, Garrett Alan Clemons, Cristiane Teresinha Citadin, Julia Langman, Drew James Smith, Luiz Henrique Matuguma, Vesna Tesic, Hung Wen Lin

Faculty, Staff and Student Publications

Alzheimer’s disease (AD) is a neurodegenerative disorder that affects more than 6.2 million Americans aged 65 and older, particularly women. Along with AD’s main hallmarks (formation of β-amyloid plaques and tau neurofibrillary tangles), there are vascular alterations that occurs in AD pathology. Adenosine A2 receptor (A2AR) is one of the key factors of brain vascular autoregulation and is overexpressed in AD patients. Our previous findings suggest that protein arginine methyltransferase 4 (PRMT4) is overexpressed in AD, which leads to decrease in cerebral blood flow in aged female 3xTg mice. We aimed to investigate the mechanism behind A2AR signaling in the …

Therapeutic Hurdles In Acute Myeloid Leukemia: Leukemic Stem Cells, Inflammation And Immune Dysfunction, Bofei Wang, Patrick K Reville, Hussein A Abbas

Faculty, Staff and Student Publications

Acute myeloid leukemia (AML) is an aggressive and highly heterogeneous hematological malignancy characterized by clonal expansion and differentiation arrest in myeloid progenitor cells. Despite advancements in chemotherapy, allogeneic hematopoietic stem cell transplantation, and post-remission maintenance therapies, the long-term survival remains unsatisfactory with high rates of relapse and refractory. These therapeutic challenges are mediated by multiple factors, including the complexity of the cellular hierarchies in AML, the interaction of leukemic stem cells (LSCs) with the bone marrow niche, inflammation, and immune evasion mechanisms. Further, the absence of specific surface markers that distinguish LSCs from normal hematopoietic stem cells, together with LSCs' …

A Gut-On-A-Chip Incorporating Human Faecal Samples And Peristalsis Predicts Responses To Immune Checkpoint Inhibitors For Melanoma, Mattia Ballerini, Serena Galiè, Punit Tyagi, Carlotta Catozzi, Hariam Raji, Amir Nabinejad, Angeli D G Macandog, Alessandro Cordiale, Bianca Ionela Slivinschi, Karol K Kugiejko, Martina Freisa, Paola Occhetta, Jennifer A Wargo, Pier F Ferrucci, Emilia Cocorocchio, Nicola Segata, Andrea Vignati, Andrey Morgun, Michela Deleidi, Teresa Manzo, Marco Rasponi, Luigi Nezi

Faculty, Staff and Student Publications

Patient responses to immune checkpoint inhibitors can be influenced by the gastrointestinal microbiome. Mouse models can be used to study microbiome-host crosstalk, yet their utility is constrained by substantial anatomical, functional, immunological and microbial differences between mice and humans. Here we show that a gut-on-a-chip system mimicking the architecture and functionality of the human intestine by including faecal microbiome and peristaltic-like movements recapitulates microbiome-host interactions and predicts responses to immune checkpoint inhibitors in patients with melanoma. The system is composed of a vascular channel seeded with human microvascular endothelial cells and an intestinal channel with intestinal organoids derived from human …

Probing Shielding Tensor Components Of Amino Acids Using Nuclear Magnetic Resonance, Shiva Agarwal

Dissertations

Chirality is fundamental to terrestrial life. While most amino acids exist as nonsuperimposable mirror images, amino acids in terrestrial life are homochiral, with the L-enantiomer being ubiquitous. The detection of an excess of L-amino acids in carbonaceous meteorites suggests that extraterrestrial processes may have contributed to this enantiomeric excess (ee). One proposed mechanism, the magnetochiral model, provides a potential explanation for this phenomenon in stellar environments characterized by strong magnetic and electric fields and the presence of relativistic leptons. According to this model, subtle differences in the electronic environments of chiral amino acids under such conditions …

Compression Of The Ulnar Nerve By The Arcade Of Struthers: Look And You Shall Find, Shady Elmaraghi, Ruby Taylor, Isaac Tung, Megan M Patterson, Susan E Mackinnon

2020-Current year OA Pubs

BACKGROUND: The arcade of Struthers was first proposed by Kane et al in 1973. Clinical investigations of this structure have been limited to small case series, focusing on the arcade as an isolated cause of compressive ulnar neuropathy. The purpose of our study was to investigate the incidence of this structure in patients undergoing ulnar nerve transposition.

METHODS: A retrospective chart review of prospectively maintained data in a single surgeon's practice was performed. Records of patients undergoing surgery for compressive ulnar neuropathy at the cubital tunnel were evaluated for documentation of a compressive arcade of Struthers. In addition, a scoping …

A Snapshot Of The Role Of Estrogen-Regulated Divergent Non-Coding Transcripts, Barbara Yang, Melina J. Sedano, Kimberly Diwa, Johnathan Dominguez, Gabriela Boisselier, Alana L. Harrison, Victoria A. Reid, Enrique I. Ramos, Maria V. Jimenez, Laura A. Sanchez-Michael

School of Medicine Publications

Recent high-throughput sequencing technologies have discovered various polymerase II transcribed transcripts. The majority of them are non-protein-coding, understudied, and poorly conserved. Noncoding transcripts are categorized based on their location in the genome and the direction in which they are transcribed; these categories classify a noncoding transcript as either antisense, intergenic, or divergent. The RNAs belonging to divergent classes consist of two transcripts, transcribed in sense and antisense direction, generated from the same promoter or locus. Multiple environmental and genetic cues can determine the regulation of these transcripts. One of the well-known signaling molecules, estrogen, has been shown to play a …

Resilient Church Planting In Global Cities : Identifying Best Practices For Building Resilience In Urban Church Planters, Branden D. Petersen

ATS Dissertations

No abstract provided.

Optimized Electrocoagulation Pre-Treatment For Fouling Reduction During Nanofiltration Of Lake Water Containing Microcystin-Lr, Thomas Mckean, Chidambaram Tharmaraiselvan, Sarah Do, S. Ranil Wickramasinghe

Chemical Engineering Faculty Publications and Presentations

Microcystin-LR (MCLR) is a toxin produced by harmful algal blooms that is emerging as a threat to drinking and recreational water systems worldwide. Nanofiltration (NF) is an effective technique for purifying contaminated water sources; however, membrane fouling caused by coexisting organic matter limits the practicality of the process. This research studies the use of an electrocoagulation (EC) pretreatment to limit fouling during the NF process. Water for this study was taken from Lake Fayetteville, a local body of water where MCLR concentrations have been recorded to be >15 µg/L. EC was performed using polyaluminum chloride as a background electrolyte at …

Little Aussie Bugs: Piloting Health Literacy Educational Resources At Early Childhood Education And Care, Ruth Wallace, Amelia Ruscoe, Leesa Costello, Gillian Kirk, Ros Sambell, Amanda Devine

Research outputs 2022 to 2026

Building health literacy in early childhood is essential for lifelong health and underpins children's learning and developmental trajectories. This study assessed the efficacy of the Little Aussie Bugs books, designed to enhance health literacy in Australian Early Childhood Education and Care (ECEC) centres. The books, featuring inclusive characters and key health literacy catchphrases, were distributed to >1200 early learning settings Australia-wide. Exploratory research design used online surveys (n = 110) and in-depth interviews with ECEC teacher participants (n = 8) to determine the compatibility of the books with ECEC contexts and practices to facilitate the delivery of health literacy education. …

Solving Multi-Objective Machine Scheduling Problem Using The Meerkat Clan Algorithm, Tahani Jabbar, Bayda Attia Khalaf, Erum Rehman

Journal of Economics and Administrative Sciences

Machine scheduling problems have become increasingly complex and dynamic. The complexity and size of the problems require the development of methods and solutions whose efficiency is measured by their ability to find acceptable results within a reasonable amount of time. Therefore, this paper addresses to propose a new mathematical model for multi objective function based on Single-machine scheduling problems  by minimizing  the discounted total weighted completion time the number of tardy jobs the maximum earliness and the maximum weighted tardiness  with release date denoted   +  which are an NP-hard. To achieve efficient solutions, a metaheuristic method (Meerkat clan algorithm (MCA)) …

The Performance Analysis Of Dynamic Investment Portfolio Insurance Strategies Based On Value At Risk, Fatima Faisal Kadim Alkhalidi, Ayad Tahir Mohammed

Journal of Economics and Administrative Sciences

The research aims to address the fundamental problem by reducing the systematic risks to which investments in the stock market are exposed and to benefit from the emerging potential provided by investment portfolio insurance strategies, through Value at Risk Based Portfolio Insurance (VBPI), using the comparative analytical approach. The research community was represented by the (ISX60) index, The data was collected through the annual reports of the Iraq Stock Exchange to form three intentional yearly conditional samples for the period (2022-2024), and several financial models were relied upon, such as Simple Ranking, Single Index, Sharpe, and Treynor Models. Several results …

The Density Of Virgin, Consumer And Environmental Plastics: An Investigation Using Gas Displacement Pycnometry, Andrew Turner, Katie Jones, Montserrat Filella

School of Geography, Earth and Environmental Sciences

Density is a fundamental property of plastics and is particularly significant in determining the transport and fate of waste plastics that enter aquatic systems. However, densities are rarely determined in the environmental literature and values employed for modelling or impacts are often unsourced or derived from secondary databases. In this study, we employ helium displacement pycnometry to determine the skeletal densities of non-porous plastics whose polymer composition had been established from manufacturer's data, resin codes or Fourier transform infrared spectrometry. Two independent, collaborative laboratories, providing measurements within 3.5 % of each other and with precisions of <1 % (as relative standard deviation), analysed a total of 42 virgin, consumer and environmental plastics consisting of ten common polymer types. Measured densities of plastics in all categories, and most notably for polybutylene terephthalate, polyethylene terephthalate, polypropylene and polyvinyl chloride, were often outside the ranges reported by a comprehensive online resource. Possible reasons for discrepancies include the occurrence of dense additives (evaluated by X-ray fluorescence analysis), the presence of inaccessible microscopic pores below a laminated surface, contamination of the main polymer by a secondary one, and structural changes on weathering. Regardless of precise causes, most results suggest that individual polymers have a broader range of densities than is generally published or considered in the literature. Accordingly, and in particular where buoyancy is critical, more precise, sample-specific measurements are recommended.

Alloparenting And Male Infant Care Behaviors In Varecia Rubra (Red-Ruffed Lemur), Noelle Wilcox

University Honors Theses

Nonhuman primates are known for their complex social structures that sometimes includes cooperative breeding strategies. In recent years there has been an increase in investigating alloparenting (infant care provided by an adult or subadult that is not a genetic parent) and male infant care, and how such behaviors contribute to the overall assistance to the extreme energetic investment and costs of the mother for the rearing of offspring. There is also debate to how male care has evolved in the primate clade, be it through the theory of kin selection or theory of mutualism. However, there is not enough research …

What Is Known About The Psychological Impacts Of Acting, And Where Are The Gaps?, Elizabeth Blaydoe

University Honors Theses

Acting has been a profession since before 400 BCE, and still is to this day through theater, television shows, movies, voice acting, and more. While studies consider the impact of acting on the audience, there are significant gaps of information for the psychological impacts this profession has. This thesis conducts a literature review of topics such as pretend play, role-play, drama therapy, and acting, to examine what is known about the topic, and a thematic analysis of fifteen publicly available interviews with a total of twenty-two actors to investigate what is known of the experience of actors. Six themes highlighted …

The Influence Of Ethnic Racial Identity On The Critical Consciousness Of Asian American College Students, Aliyah Sukkram

University Honors Theses

Ethical and racial identity (ERI) and critical consciousness play crucial roles in how youth of color understand themselves, contextualize their racialized experiences, and navigate oppressive social conditions. However, there has been a lack of research on both constructs in the lives of Asian American youth. Thus, this thesis aims to study the influence of ERI on the critical consciousness of Asian American college students by conducting semi-structured interviews. ERI was found to influence critical reflection and critical action but not political efficacy. The relationship between ERI and critical consciousness is understood through how youth engage with their ERI and contextualize …

American Consumer Culture And Social Media Polarization: Analyzing Twentieth Century Trends And Their Effects On Twenty First Century Politics, Max Lally

University Honors Theses

The emergence of social media as a mainstream communication technology during the early twenty first century has had an outsized impact on the United States' political system. By analyzing trends in American culture and economics beginning in the decades following the end of World War Two, the circumstances that shaped social media during its development can be tied to long term historical changes. Shifts away from traditional models of community and towards rising individualism reshaped communication with both political allies and opponents. Generational shifts in political attitudes have heightened these divisions as well, as younger and liberal perspectives challenge traditional, …

Gianjara's Ribs: The Ecosystem, Body, Animalia, And Politic, Linus Gomez Ellis

University Honors Theses

This work is a series of four six foot tall full-body portraits of people, which are displayed alongside an actual, physical item or garment present in the associated painting. The purpose of this is to blur the line between the fiction of the individuals depicted and the viewer. By creating "portals" through which to view them as equals in stature, Linus Gomez Ellis is taking something that can be seen in the ecosystem of this fiction and making it into an objective reality. The most enduring, well preserved, and eternal pieces of history and anthropology are tall tales. This ecosystem, …

Exploring Vaccine Efficacy And Safety For Ms Patients On Immunosuppressant Treatment In Light Of The Covid-19 Pandemic, Rain Pross

University Honors Theses

Multiple sclerosis (MS) is a chronic neurodegenerative autoimmune disorder affecting approximately 2.8 million people worldwide. Despite decades of research to understand the molecular mechanisms of MS pathology, little is known about why the neurodegeneration caused by this illness occurs. For this reason, current treatments for MS target two broader aspects of the disease: relapse management and prevention via disease-modifying therapies. These treatments often involve immunosuppressive mechanisms, creating vulnerability to infectious diseases that became particularly concerning during the COVID-19 pandemic. This literature review examines the safety and efficacy of vaccination for MS patients on immunosuppressant therapies, highlighting the novel mRNA vaccines …

Messy Kitchens, Clean Hands, Emily Camp

University Honors Theses

Contemporary industrial food systems are well-tuned towards their primary goal: the efficient production of cheap food to generate high profits. Perhaps nowhere is this more acute than in the production of industrial meat. Here, workers, consumers and animals are trapped in a system that ignores their interests in order to achieve maximum profit for shareholders. But how does modern industrial meat production remain mostly hidden from view?

This interdisciplinary art and cultural history thesis explores this question by looking to the kitchen, and more specifically, to its decor. These decorative objects both reflect and enable a complex ideological transformation. I …

Penicillin: The Burdensome Physiology Of Β-Lactamase, Lena Uyen-Thi Ngo

University Honors Theses

This thesis is a literature review investigating the effects of overusing penicillin and the nonadherence to proper treatment on the evolutionary changes in a bacterium's resistance. Drawing upon the history, present, and future of penicillin research, the examination analyzes how penicillin came to be, how bacteria developed their resistance, and how scientists are combating this issue. In doing so, the study investigates the historical timeline regarding the discovery of penicillin as an antibiotic. Furthermore, the review will examine the scientific changes within a bacterium's physiology to develop its antibiotic resistance. Finally, the review will delve into what scientists are currently …

The Impact Of Emotions On Informal Learning Experiences In The Workplace, Jennifer L. Walavalkar

University Honors Theses

This study investigates the role of emotion--specifically valence and arousal--on informal learning experiences in the workplace. As remote and flexible work environments grow, informal learning has become the dominant method through which employees acquire new skills. Drawing on theories such as Cognitive Load Theory and Ego Depletion Theory, this research explores how emotions influence cognitive processes critical to learning, such as attention, memory, and self-regulation. Prior literature suggests that both positive and negative affect (valence) impact learning outcomes, but the specific influence of emotional intensity (arousal) in informal learning remains underexplored. Using a longitudinal survey method, ten employees at Portland …

Indigenous Language Revitalization: The Klamath And Cherokee Tribes, Josephine Stapleton

University Honors Theses

In recent decades, there have been initiatives to revitalize Indigenous languages all across the globe. This paper explores the efforts of two Native American tribes to revitalize their Indigenous languages in the face of historical and ongoing colonial pressures. Models for language revitalization necessarily differ based on an Indigenous group’s specific historical, cultural, and even geographical contexts, which makes the development of standardized models for Indigenous Language Revitalization challenging. This literature review will examine how the Cherokee Nation and the Klamath Tribes are implementing language revitalization on a tribal level in ways that address each tribe’s unique historical successes and …

An Erotics Manifesto: Developing Critical Feminist Theory Through Erotic Fiction, Cairn Des Lions

University Honors Theses

Erotic literature provides a unique lens through which feminists can develop theory because it reveals the natures of our gendered relations in the contexts of our desires. By either writing or critically reading erotic literature, feminists can explore questions about what and why we desire, how we act upon our desires, and what our desires and actions reveal about our relationships to each other. This thesis uses feminist theory to analyze the potential of fiction focused on eroticism to aid in rethinking gender and sexuality norms.