Digital Commons Network^™

Prmt5 Interacting Partners And Substrates In Oligodendrocyte Lineage Cells, David K. Dansu, Jialiang Liang, Ipek Selcen, Haiyan Zheng, Dirk F. Moore, Patricia Casaccia

Advanced Science Research Center

The protein arginine methyl transferase PRMT5 is an enzyme expressed in oligodendrocyte lineage cells and responsible for the symmetric methylation of arginine residues on histone tails. Previous work from our laboratory identified PRMT5 as critical for myelination, due to its transcriptional regulation of genes involved in survival and early stages of differentiation. However, besides its nuclear localization, PRMT5 is found at high levels in the cytoplasm of several cell types, including oligodendrocyte progenitor cells (OPCs) and yet, its interacting partners in this lineage, remain elusive. By using mass spectrometry on protein eluates from extracts generated from primary oligodendrocyte lineage cells …

The Therapeutic Effect Of Exogenous Melatonin On Depressive Symptoms: A Systematic Review And Meta-Analysis, Cancan Li, Dandan Ma, Min Li, Tao Wei, Xuan Zhao, Yuanyuan Heng, Delong Ma, Enoch Odame Anto, Yanbo Zhang, Mingyun Niu, Wangxin Zhang

Research outputs 2022 to 2026

Background: Depression-related mortality and morbidity pose growing public health burdens worldwide. Although the therapeutic effect of exogenous melatonin on depression has been investigated, findings remain inconsistent. We conducted this systematic review and meta-analysis to clarify the effectiveness of melatonin in the treatment of depression, including primary and secondary depression symptoms. Methods: We searched the online databases of PubMed, EMBASE, and the Cochrane Library for original studies published up to May 2021. We used STATA 14.0 software to synthesize the results of included studies. To evaluate the effectiveness of melatonin, we calculated the standardized mean differences (SMDs) and 95% confidence intervals …

Department Of Hous. Preserv. & Dev. Of The City Of N.Y. V. Rosenfeld

All Decisions

No abstract provided.

Asphaltene Or Polyvinylchloride Waste Blended With Cement To Produce A Sustainable Material Used In Nuclear Safety, Heba A. Esawii, Hosam M. Saleh, Ibrahim I. Bondouk, Elsayed Salama

Faculty Journal Articles

The current research uses sustainable methods to preserve the environment, such as exploiting municipal or industrial waste that may harm the environment. The wreckage of polyvinyl chloride (PVC) pipes and asphaltene are used as additives to cement to improve its mechanical properties, while stabilizing the radioactive waste resulting from the peaceful uses of nuclear materials, or enhancing its radiation shielding efficiency. New composites of Portland cement with ground PVC or asphaltene up to 50% are investigated. Fast neutron removal cross-section (ƩR) and gamma shielding parameters, such as mass attenuation coefficient (MAC), half-value layer (HVL), effective atomic number (Zeff), and exposure …

The Economic And Business Case For Ensuring High-Quality Childcare And Preschool, Timothy J. Bartik

Presentations

No abstract provided.

Stem Showcase: The Loudenslager Steam Center, Rebecca Richardson

STEM Month

Loudenslager Elementary School has a unique approach to teaching STEM education. The school library has been converted into a STEAM center. Each classroom is scheduled to visit the STEAM center for one class period weekly. The STEAM center is equipped with a variety of resources including robots, digital textiles (ex. 3D printer), Ipads, building materials, Promethean board, flexible seating, STEAM-related reading materials, agriculture materials, and a wildlife conservation station.

The curriculum is ever-evolving to meet the changing fields of STEAM. A new topic is introduced monthly focusing on academic vocabulary, hands-on student experience, and career exploration.

Some topics that have …

Importance Of Proactive Safety Analysis For Corridor Planning, Martin Guttenplan, Houssam Ghandour

Purdue Road School

A proactive safety analysis approach assists decision makers in addressing high-risk crash elements, particularly on low-volume roadways. Using corridor planning best practices and lessons learned, this presentation will explore creative and proactive approaches for holistic planning solutions. It will also validate 2020 pandemic crash data accuracy and highlight prioritization methodologies and AASHTO and FHWA guided countermeasures applied to at-risk locations.

Safety Analysis For All Projects, Mike Holowaty, Taylor Ruble

Purdue Road School

The reduction of roadway fatalities and serious injuries is a key goal of INDOT. In order to meet the vision of zero roadway deaths, the analysis of motor vehicle crash risk is an important part of project development. This session will discuss how INDOT is bringing appropriate safety analysis practices to the development of engineering assessment (scoping) preparation and into the design process.

A Substructure-Based Screening Approach To Uncover N-Nitrosamines In Drug Substances, Yu-Ting Kao, Shu-Fen Wang, Meng-Hsiu Wu, Shwu-Huey Her, Yi-Hsuan Yang, Chung-Hsien Lee, Hsiao-Feng Lee, An-Rong Lee, Li-Chien Chang, Li-Heng Pao

Journal of Food and Drug Analysis

Drug substances are at risk of contamination with N-nitrosamines (NAs), well-known carcinogenic agents, during synthesis processes and/or long-term storage. Therefore, in this study, we developed an efficient data-based screening approach to systemically assess marketed products and investigated its scalability for benefiting both regulatory agencies and pharmaceutical industries. A substructure-based screening method employing DataWarrior, an open-source software, was established to evaluate the risks of NA impurities in drug substances. Eight NA substructures containing susceptible amino sources for N-nitrosation have been identified as screening targets: dimethylamine (DMA), diethylamine, isopropylethylamine, diisopropylamine, N-methyl-2-pyrrolidone, dibutylamine, methylphenylamine, and tetrazoles. Our method detected 192 drug …

Repurposing Lansoprazole And Posaconazole To Treat Leishmaniasis: Integration Of In Vitro Testing, Pharmacological Corroboration, And Mechanisms Of Action, Yash Gupta, Steven Goicoechea, Jesus G. Romero, Raman Mathur, Thomas R. Caulfield, Daniel P. Becker, Ravi Durvasula, Prakasha Kempaiah

Journal of Food and Drug Analysis

Leishmaniasis remains a serious public health problem in many tropical regions of the world. Among neglected tropical diseases, the mortality rate of leishmaniasis is second only to malaria. All currently approved therapeutics have toxic side effects and face rapidly increasing resistance. To identify existing drugs with antileishmanial activity and predict the mechanism of action, we designed a drug-discovery pipeline utilizing both in-silico and in-vitro methods. First, we screened compounds from the Selleckchem Bio-Active Compound Library containing ~1,622 FDA-approved drugs and narrowed these down to 96 candidates based on data mining for possible anti-parasitic properties. Next, we completed preliminary in-vitro testing …

Effect Of Repeated Shengmai-San Administration On Nifedipine Pharmacokinetics And The Risk/Benefit Under Co-Treatment, Hong-Jaan Wang, Elise Chia-Hui Tan, Tzu-Yi Chiang, Wei-Ching Chen, Chien-Chang Shen, Yune-Fang Ueng

Journal of Food and Drug Analysis

Herbal interactions with nifedipine/felodipine through cytochrome P450 (CYP) 3A inhibition is significant in humans. Shengmai-San (SMS), a three-herbal formula of Chinese medicine, is commonly prescribed in Asia populations for cardiovascular disorders. This study aimed to elucidate the impact of SMS on nifedipine/felodipine treatment by the findings from rat pharmacokinetic study of nifedipine to the retrospective cohort study of patients with hypertension. The 3-week SMS treatment increased the systemic exposure to nifedipine by nearly two-fold and decreased nifedipine clearance by 39% in rats. Among the ingredients of SMS component herbs, schisandrin B, schisantherin A, and methylophiopogonanone A, inhibited the nifedipine oxidation …

The Antihyperlipidemic Equivalent Combinatorial Components From Peel Of Citrus Reticulata ‘Chachi’, Ping-Ting Xiao, Yu-Jia Kuang, Shi-Yu Liu, Zhi-Shen Xie, Jin-Hua Hao, E-Hu Liu

Journal of Food and Drug Analysis

Since the combinatorial components responsible for the antihyperlipidemic activity of Citrus reticulata‘Chachi’ (CRC) peels remains unclear, we herein developed a bioactive equivalence oriented feedback screening method to discover the bioactive equivalent combinatorial components (BECCs) from CRC peels. Using palmitic acid (PA)- stimulated hepatocyte model, a combination of five polymethoxyflavones (PMFs) including tangeretin, sinensetin, nobiletin, 5,7,8,4′-tetramethoxyflavone and 3,5,6,7,8,3',4'-heptamethoxyflavone was identified to be responsible for the antihyperlipidemic effect of CRC peels. Via evaluation of combination effect by combination index (CI), these 5 PMFs were found to take effect via a synergistic mode. Our data indicated that the antihyperlipidemic mechanism of PMF …

Ulva Prolifera Polysaccharide Exerts Anti-Obesity Effects Via Upregulation Of Adiponectin Expression And Gut Microbiota Modulation In High-Fat Diet-Fed C57bl/6 Mice, Hai-Ching Pung, Wei-Sheng Lin, Yi-Chen Lo, Cheng-Chih Hsu, Chi-Tang Ho, Min-Hsiung Pan

Journal of Food and Drug Analysis

Obesity is characterized by metabolic disorder and accompanying an altered and less diverse gut microbiota composition during a fat-enriched diet. Recent studies indicated that sulphated polysaccharide prevents high-fat diet (HFD) induced obesity, reduces metabolic disorder, and restores the gut microbiota. However, there are few studies about Ulva prolifera polysaccharide (UPP) may induce anti-obesogenic effects. Therefore, the present study investigates the enzymatic extracted UPP effects in HFD-fed mice. The results showed that UPP considerably slowed down the HFD-induced weight gain and improved metabolic disorders in HFD-fed mice. Notably, the effects were associated with lower body weight gain, reduced adipose tissue hypertrophy, …

Direct Immersion Solid-Phase Microextraction Combined With Ambient Ionization Tandem Mass Spectrometry To Rapidly Distinguish Pesticides In Serum For Emergency Diagnostics, Hung Su, Hsin-Hua Lin, Lin-Jhen Su, Chia-Chen Lin, Zong-Han Jiang, Shiang-Jin Chen, Jentaie Shiea, Chi-Wei Lee

Journal of Food and Drug Analysis

Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care platform as rapid analyses could be done in tiny sample volumes with satisfactory recovery (89.46%–116.32%), precision (covariance < 20%), sensitivity (LOD < 0.1 µg/mL), turnaround time (< 5 minutes), and linearity (R² = 0.9827–0.9992) within 0.1–100 µg/mL.

The Next Generation Beneficial Actions Of Novel Probiotics As Potential Therapeutic Targets And Prediction Tool For Metabolic Diseases, Hsin-Lin Cheng, Gow-Chin Yen, Shih-Chien Huang, Shiuan-Chih Chen, Chin-Lin Hsu

Journal of Food and Drug Analysis

The prevalence of metabolic disease has rising and affected over 1,000 million populations globally. Since the metabolic disease and its related complication are board, it has become the major health hazard of modern world. However, Long term medication of metabolic disease may cause serious side effects and risk for adverse health problems. Recently, emerging studies focus on exploring the mechanistic details of metabolic state in disease development and progression. Gut bacteria ecosystem was considered to play a pivotal role in regulating energy homeostasis and great associated with the development of metabolic disease. Accumulated evidences indicated that Akkermansia muciniphila, Faecalibacterium prausnitzii, …

Roadhat D, Version 4.1 - Changes From Roadhat 3, Thomas E. Ford

Purdue Road School

This session will describe the subtle and not-so-subtle changes between the new RoadHAT version 4 and previous versions and also discuss some recommended choices in using RoadHAT 4 for the various road facility types.

Climate Change, Air Pollution, And Children’S Mental Health, Wuyue Yu, Michael Weitzman

Journal of Applied Research on Children: Informing Policy for Children at Risk

Climate change and air pollution are twin global public health challenges of high priority. Sharing linked causes and solutions, both climate change and air pollution are suggested by a growing body of research to inflict negative mental health outcomes through multiple mechanisms. However, there are still gaps in understanding the links between climate change, air pollution and mental health, especially in children and adolescents, who are particularly at-risk. Current findings are summarized and their implications for future research as well as public health policies are discussed.

We Are ... Marshall, March 16, 2022, Office Of Marshall University Communications

We Are ... Marshall: the Newsletter for Marshall University 1999-2023

No abstract provided.

Benthic Biolayer Structure Controls Whole-Stream Reactive Transport, Kevin R. Roche, Marco Dentz

Civil Engineering Faculty Publications and Presentations

Hyporheic zone reaction rates are highest just below the sediment-water interface, in a shallow region called the benthic biolayer. Vertical variability of hyporheic reaction rates leads to unexpected reaction kinetics for stream-borne solutes, compared to classical model predictions. We show that deeper, low-reactivity locations within the hyporheic zone retain solutes for extended periods, which delays reactions and causes solutes to persist at higher concentrations in the stream reach than would be predicted by classical approaches. These behaviors are captured by an upscaled model that reveals the fundamental physical and chemical processes in the hyporheic zone. We show how time scales …

Transcriptional Control Of Retinal Ganglion Cell Death After Axonal Injury., Stephanie B Syc-Mazurek, Hongtian Stanley Yang, Olivia J Marola, Gareth R Howell, Richard T Libby

Faculty Research 2022

Injury to the axons of retinal ganglion cells (RGCs) is a key pathological event in glaucomatous neurodegeneration. The transcription factors JUN (the target of the c-Jun N-terminal kinases, JNKs) and DDIT3/CHOP (a mediator of the endoplasmic reticulum stress response) have been shown to control the majority of proapoptotic signaling after mechanical axonal injury in RGCs and in other models of neurodegeneration. The downstream transcriptional networks controlled by JUN and DDIT3, which are critical for RGC death, however, are not well defined. To determine these networks, RNA was isolated from the retinas of wild-type mice and mice deficient in Jun, Ddit3, …

Adult Mouse Fibroblasts Retain Organ-Specific Transcriptomic Identity., Elvira Forte, Mirana Ramialison, Hieu T Nim, Madison Mara, Jacky Y Li, Rachel Cohn, Sandra Daigle, Sarah Boyd, Edouard G Stanley, Andrew G Elefanty, J Travis Hinson, Mauro W Costa, Nadia Rosenthal, Milena B Furtado

Faculty Research 2022

Organ fibroblasts are essential components of homeostatic and diseased tissues. They participate in sculpting the extracellular matrix, sensing the microenvironment, and communicating with other resident cells. Recent studies have revealed transcriptomic heterogeneity among fibroblasts within and between organs. To dissect the basis of interorgan heterogeneity, we compare the gene expression of murine fibroblasts from different tissues (tail, skin, lung, liver, heart, kidney, and gonads) and show that they display distinct positional and organ-specific transcriptome signatures that reflect their embryonic origins. We demonstrate that expression of genes typically attributed to the surrounding parenchyma by fibroblasts is established in embryonic development and …

The Apoeε3/Ε4 Genotype Drives Distinct Gene Signatures In The Cortex Of Young Mice, Kate E Foley, Amanda A Hewes, Dylan Garceau, Kevin P Kotredes, Gregory W. Carter, Michael Sasner, Gareth R Howell

Faculty Research 2022

Introduction: Restrictions on existing APOE mouse models have impacted research toward understanding the strongest genetic risk factor contributing to Alzheimer's disease (AD) and dementia, APOE^ε4 , by hindering observation of a key, common genotype in humans - APOE^ε3/ε4 . Human studies are typically underpowered to address APOE^ε4 allele risk as the APOE^ε4/ε4 genotype is rare, which leaves human and mouse research unsupported to evaluate the APOE^ε3/ε4 genotype on molecular and pathological risk for AD and dementia.

Methods: As a part of MODEL-AD, we created and validated new versions of humanized APOE^ε3/ε3 and APOE^{ε4/ε4 …}

Board Of Pharmacy, Sarah Dahm, Steven Funk, Bridget Fogarty Gramme, Karen Thomas Stefano

California Regulatory Law Reporter

No abstract provided.

Veterinary Medical Board, Stella Gerson, Bridget Fogarty Gramme, Karen Thomas Stefano

California Regulatory Law Reporter

No abstract provided.

A Course-Based Undergraduate Research Experience (Cure) In Biology: Developing Systems Thinking Through Field Experiences In Restoration Ecology, Erin Stanfield, Corin D. Slown, Quentin Sedlacek, Suzanne E. Worcester

Biology, Agriculture and Chemistry Faculty Publications and Presentations

In this study, we posit that integrating design-based research (Scott et al., 2020) and nature-based learning (Jordan and Chawla, 2019) approaches in biology can lead to gains in holistic systemic thinking (Verhoeff et al., 2018), thinking like a scientist, and greater equity for historically unrepresented student groups. While such experiences can be achieved through participation in research apprenticeships and internships, course-based undergraduate research experiences (CUREs) have a greater potential to impact a broader range of students. This paper invites not only the students to broaden their thinking to expand their horizons, but also faculty to broaden their design and implementation …

Case Study: Evaluating The Impact Of Preference On The Efficacy Of The High Probabilityinstructional Sequence, Alexandria Torres

USF Tampa Graduate Theses and Dissertations

Non-compliance is a common behavior amongst children with ASD (Esch & Fryling, 2013). Non-compliance is known to have multiple negative consequences, including the hinderance of acquisition of skills which then can result in academic and social deficits (Belfiore et al., 2008; Esch & Fryling, 2013; Lee et al., 2006). Therefore, the purpose of this study was to compare two high-probability instructional sequence (HPIS) conditions, one in which highly preferred high-probability instructions (High-P) were included in the HPIS and another in which the HPIS included non-preferred High-P instructions. Furthermore, this study used a questionnaire to assess sociality validity of HPIS from …

Effects Of Pore-Forming Peptides (Melittin And Magainin 2) On The Phospholipid Bilayer Interior, Elmukhtar Ehmed Alhatmi

Dissertations and Theses

Antimicrobial peptides (AMPs) are one of the most promising solutions to drug-resistant bacteria. Melittin and magainin 2 are two of the most representative and extensively studied AMPs. In this research, I investigated the interaction of these two AMPs with three models of cell membranes: 80% POPC 20% POPG, 40%POPC 40% POPE and 20% POPG, and 80%POPC 20%POPG plus 30% mole fraction of cholesterol. Time-resolved fluorescence emission and fluorescence anisotropy decays of the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) were analyzed to determine the effects of AMPs on the bilayer headgroup packing and changes in the interior of the phospholipid bilayer during the …

Word Imageability Is Associated With Expressive Vocabulary In Children With Autism Spectrum Disorder, Kimberly R. Lin, Lisa Wisman Weil, Audrey Thurm, Catherine Lord, Rhiannon Luyster

Emerson Authors, Researchers, & Creators

Throughout typical development, children prioritize different perceptual, social, and linguistic cues to learn words. The earliest acquired words are often those that are perceptually salient and highly imageable. Imageability, the ease in which a word evokes a mental image, is a strong predictor for word age of acquisition in typically developing (TD) children, independent of other lexicosemantic features such as word frequency. However, little is known about the effects of imageability in children with autism spectrum disorder (ASD), who tend to have differences in linguistic processing and delayed language acquisition compared to their TD peers. This study explores the extent …

Imaginary Landscapes, Richard Oliver Reed

Theses and Dissertations

Worldbuilding is a common practice that informs and enriches our everyday experience. My practice of worldbuilding investigates the conception of an interior landscape. Through my cultural and material influences I construct spaces that highlight imagination and escapism. These overlooked practices serve an important mediative function in our lives. In a digital world where our play spaces and workspaces overlap it becomes harder to distinguish between them. How do we differentiate the real from the unreal? Perhaps the distinction isn’t as clear as it seems.

The Current - Volume 32 Issue 21, Nova Southeastern University

Student Newspapers

No abstract provided.