Digital Commons Network^™

Gamma Interferon Induces Monocytoid Differentiation In The Hl-60 Cell Line., Edward D. Ball, Paul M. Guyre, Li Shen, John M. Glynn, Charles R. Maliszewski, Paul E. Baker, Michael W. Fanger

Dartmouth Scholarship

We investigated the ability of purified, recombinant DNA-derived interferons (IFN) to induce phenotypic changes in cells of the HL-60 promyelocytic leukemia cell line. Changes in cell surface markers detected by monoclonal antibodies as well as morphologic, histochemical, and functional changes were monitored. We found that gamma-IFN, but not alpha- or beta-IFN, induced the expression of antigens characteristic of monocytes and granulocytes (AML-2-23, 63D3, and 61D3), as well as changes in morphology consistent with monocytoid differentiation. These included induction of alpha-naphthyl acetate esterase, increased cell size, and a decrease in azurophilic granules. The gamma-IFN dose dependency and time course of the …

Triggering Of The T3-Ti Antigen-Receptor Complex Results In Clonal T-Cell Proliferation Through An Interleukin 2-Dependent Autocrine Pathway., Stefan C. Meuer, Rebecca E. Hussey, Doreen A. Cantrell, James C. Hodgdon, Stuart F. Schlossman, Kendall A. Smith, Ellis L. Reinherz

Dartmouth Scholarship

Human T-cell clones and anti-T-cell-receptor antibodies (clonotypic) directed at surface receptors for antigen (T3-Ti molecular complex) as well as anti-interleukin 2 (IL-2) and anti-IL-2-receptor antibodies were utilized to investigate the mechanism by which alloantigens or antigen plus self-major histocompatibility complex (MHC) (i.e., physiologic ligand) trigger specific clonal proliferation. Soluble or Sepharose-bound anti-Ti monoclonal antibodies, like physiologic ligand, enhanced proliferative responses to purified IL-2 by inducing a 6-fold increase in surface IL-2 receptor expression. In contrast, only Sepharose-bound anti-Ti or physiologic ligand triggered endogenous clonal IL-2 production and resulted in subsequent proliferation. The latter was blocked by antibodies directed at either …

Interferon Gamma Blocks The Growth Of Toxoplasma Gondii In Human Fibroblasts By Inducing The Host Cells To Degrade Tryptophan., E. R. Pfefferkorn

Dartmouth Scholarship

Treatment of human fibroblasts with human recombinant gamma interferon blocked the growth of Toxoplasma gondii, an obligate intracellular protozoan parasite. Growth of the parasite was measured by a plaque assay 7 days after infection or by the incorporation of [3H]uracil 1 or 2 days after infection. The antitoxoplasma activity induced in the host cells by gamma interferon was strongly dependent upon the tryptophan concentration of the medium. Progressively higher minimal inhibitory concentrations of gamma interferon were observed as the tryptophan concentration in the culture medium was increased. Treatment with gamma interferon did not make the cells impermeable to tryptophan. The …

G84-717 Fleas And Their Management (Revised January 1993), Shripat T. Kamble, David L. Keith

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide provides information on flea biology and how to solve flea problems.

Fleas are among the most annoying pests known to humans. In ancient times, fleas were even indicted, found guilty and sentenced to condemnation. Today, the verdict remains the same.

In Nebraska, fleas are common household pests attacking dogs, cats and sometimes people. In addition to these, several other flea species occur in Nebraska, feeding principally on rodents and occasionally on poultry and livestock. The information provided in this publication will focus primarily on cat and dog fleas, as they are the most commonly encountered species.

Recombinant Immune Interferon Increases Immunoglobulin G Fc Receptors On Cultured Human Mononuclear Phagocytes, Paul M. Guyre, Peter M. Morganelli, Renee Miller

Dartmouth Scholarship

Although recent studies suggest that interferons can increase the number of IgG Fc receptor (FcR gamma) sites on mouse macrophages, direct assessment of similar effects on human mononuclear phagocytes is lacking. We therefore measured the specific binding of 125I- and fluorescein-labeled IgG1 to human monocytes and leukemic cell lines after culture in vitro with highly purified human interferons. We report that natural and recombinant human gamma-interferon causes a dramatic (nearly 10-fold) increase in the number of FcR gamma on normal human monocytes and on the human cell lines HL-60 and U-937. Alpha and beta-interferons cause a modest but significant increase …

Kill That Spider (Reflections From Lebanon), Adnan Hakim

The Prairie Light Review

No abstract provided.

Monoclonal Antibodies To Novel Myeloid Antigens Reveal Human Neutrophil Heterogeneity., Edward D. Ball, Robert F. Graziano, Li Shen, Michael W. Fanger

Dartmouth Scholarship

Three cytotoxic murine monoclonal antibodies that recognize myeloid-specific antigens have been produced by immunization with normal human neutrophils or myeloblasts from a patient with acute myelomonocytic leukemia. Two of these, PMN 6 and PMN 29, are specific for neutrophils; the third monoclonal antibody, AML-2-23, is reactive with the majority of normal monocytes as well as a subpopulation of mature neutrophils. Although neutrophils from all individuals tested expressed these antigens, cytofluorographic analysis revealed that the percentage of cells bearing the PMN 6 and AML-2-23 antigens varied among individuals. Significant additional heterogeneity in the density of each antigen among antigen-bearing cells was …

Kill That Spider (Reflections From Lebanon), Adnan Hakim

The Prairie Light Review

No abstract provided.

Proteins Antigenically Related To The Human Erythrocyte Glucose Transporter In Normal And Rous Sarcoma Virus-Transformed Chicken Embryo Fibroblasts., Donald W. Salter, Stephen A. Baldwin, Gustav E. Lienhard, Michael J. Weber

Dartmouth Scholarship

Antibody raised against the purified human erythrocyte glucose transporter specifically precipitated four proteins from normal and Rous sarcoma virus-transformed chicken embryo cells: a major protein of Mr 41,000 and minor proteins of Mr 68,000, 73,000, and 82,000. The Mr 41,000 and 82,000 proteins were found only in a membrane fraction, not in the soluble fraction, and displayed a heterogeneous mobility on NaDodSO4/polyacrylamide gel electrophoresis, suggesting glycosylation. The Mr 41,000 and 82,000 proteins were increased in amount after malignant transformation in direct proportion to the increase in hexose transport rate, and the increase was dependent on the expression of the src …

The Roles Of Dna Polymerases Alpha, Beta, And Gamma In Dna Repair Synthesis Induced In Hamster And Human Cells By Different Dna Damaging Agents., M. R. Miller, D. N. Chinault

Faculty & Staff Scholarship

No abstract provided.

Microtubule Dynamics And Glutathione Metabolism In Phagocytizing Human Polymorphonuclear Leukocytes., B R Burchill, J M Oliver, C B Pearson, E D Leinbach, R D Berlin

SoM Articles

Glutathione oxidants such as tertiary butyl hydroperoxide were shown previously to prevent microtubule assembly and cause breakdown of preassembled cytoplasmic microtubules in human polymorphonuclear leukocytes. The objectives of the present study were to determine the temporal relationship between the attachment and ingestion of phagocytic particles and the assembly of microtubules, and simultaneously to quantify the levels of reduced glutathione and products of its oxidation as potential physiological regulators of assembly. Polymorphonuclear leukocytes from human peripheral blood were induced to phagocytize opsonized zymosan at 30 degrees C. Microtubule assembly was assessed in the electron microscope by direct counts of microtubules in …

Patterns Of Fatty Acid Release From Endogenous Substrates By Human Platelet Homogenates And Membranes, A Derksen, P Cohen

Faculty, Staff and Students Publications

We describe a method for measuring the release of fatty acids from endogenous substrates of human platelet homogenates and membranes. The method depends on the availability of lipids whose fatty acids are odd-chained and therefore suitable as internal reference compounds that, at the time of lipid extraction, can be added to an incubation to permit subsequent quantification of the content of free fatty acids or fatty acids esterified to specific lipids. We found four types of lipolytic activities in human platelets. In homogenates at pH 4.0 a triglyceride lipase operated as shown by the synchrony of triglyceride degradation and release …

Human Being: The Boundaries Of The Concept, Lawrence C. Becker

Philosophy Faculty Scholarship

No abstract provided.

Drug Abuse Law Abuse And The Eighth Amendment New York’S 1973 Drug Legislation And The Prohibition Against Cruel And Unusual Punishment, Robert E. Glanville

Cornell Law Review

No abstract provided.

Chemotactic Factor Inactivator In Normal Human Serum., J L Berenberg, P A Ward

SoM Articles

Normal human serum contains an inactivator of chemotactic factors for neutrophilic leukocytes. The chemotactic factor inactivator (CF-I) remains soluble when serum is fractionated with ammonium sulfate (at 45% saturation), directly and irreversibly inactivates chemotactic factors, and it has a broad spectrum of activity as indicated by its inactivation of the chemotactic fragments of human C3 and C5 (third and fifth components of complement), C5̅6̅7̅, and the bacterial chemotactic factor derived from Escherichia coli. CF-I appears as a biphasic activity according to preparative techniques of sucrose density ultracentrifugation, electrophoresis, and gel filtration. Studies on the interaction of CF-I with the radiotagged …

The Doctor-Patient Relationship Revisited. An Analysis Of The Placebo Effect., Herbert M. Adler, Md, Van B. Hammett. Md

Department of Psychiatry and Human Behavior Faculty Papers

An overview of prescientific medicine, evolution, and individual human development is presented in an attempt to discover the generic factors operating in all interpersonal therapies. We hypothesize that the placebo effect rests on the universal human need for a group and, by symbolic extension, a system.

Adaptive Changes Of Some Enzyme Activities In Rats And Humans To Dietary Protein, Jung Ja Kim

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A study of the adaptive changes of some enzyme activities to the dietary protein intake was made in the liver, kidney, and pancreas of rats and serum of humans.

The rats fed the 40 per cent casein diet had a higher rate of weight gain and the weights of the liver and kidney were higher than in the rats fed the 10 per cent casein diet.

Three enzymes involved in the elimination of excess nitrogen from the body were found to show a similar response to increased dietary protein intake. These enzymes were: D-amino acid oxidase in the kidneys, arginase …

A New Surgical Approach For Vagotomy, Diaphragmatic Herniotomy, Splenectomy, And Operations Upon The Gastro-Esophageal Area Through The Left Sub-Diaphragmatic Extraperitoneal Space, H. A. Davis

Medical Arts and Sciences: A Scientific Journal of the College of Medical Evangelists

No abstract provided.

The Incidence Of Avian Tuberculosis In Mammals Other Than Swine, L. Van Es, H. M. Martin

Nebraska Agricultural Experiment Station: Historical Research Bulletins

The recognition of a very widespread and intense distribution of avian tuberculosis on the farms of a wide area of the United States, as well as the involvement of a mammalian species also, brought to the foreground the question whether or not the great prevalence of poultry tuberculosis could be of importance also from a public health point of view. This phase of the problem not only pertains to the people on the farms but likewise is of fundamental importance to the consumers of poultry produce. European authors had already identified the avian tubercle bacillus in human lesions while in …

Studies On The Ascaris Lumbricoides, H. M. Martin

Nebraska Agricultural Experiment Station: Historical Research Bulletins

The Ascaris lumbricoides, in one phase or another, has attracted the attention of parasitologists for many years, and during the last decade this parasite has been studied by a large number of investigators. The recent observations have been, to a great extent, directed along one line, the study of its life history. These investigators have established two important points: (a) That the life history of Ascaris is more complex than was supposed, as it has been found that a vasculo-pulmonary circuit of the larvae is necessary before they settle down in the intestine, where they develop to the adult …