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Cytotoxicity

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Cytotoxicity Of Diepoxybutane And Epichlorohydrin In Relation To Stages Of The Cell Cycle, Megan L. Watts Jan 2008

Cytotoxicity Of Diepoxybutane And Epichlorohydrin In Relation To Stages Of The Cell Cycle, Megan L. Watts

Honors Theses

Work conducted in the Millard Biochemistry Research Laboratory examines the dual nature of molecules as carcinogens and anti-tumor agents through the molecular mechanisms of duplex DNA damage by bifunctional alkylating agents. Diepoxybutane (DEB) and epichlorohydrin (ECH) are polar molecules that form covalent DNA interstrand lesions by cross-linking the N7 position of deoxyguanosine residues. A recent experiment indicated that ECH preferentially targets nuclear DNA over mitochondrial DNA, whereas DEB shows similar rates of lesion formation for both loci. It was concluded that preferential targeting of nuclear DNA results from relatively poor uptake of ECH across the mitochondrial membrane. The objective of …

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Studies On The Effect Of Maleic Hydrazide On Root Tip Cells And Pollen Fertility In Trigonella Foenum-Graecum L., Farha Jabee, Mohammad Yunus Khalil Ansari, Danish Shahab Jan 2008

Studies On The Effect Of Maleic Hydrazide On Root Tip Cells And Pollen Fertility In Trigonella Foenum-Graecum L., Farha Jabee, Mohammad Yunus Khalil Ansari, Danish Shahab

Turkish Journal of Botany

In the present investigation, the effect of maleic hydrazide (MH) has been studied on seed germination, mitosis, and pollen fertility of Trigonella foenum-graecum L. to test its cytotoxicity. Six different concentrations (0.01%, 0.02%, 0.03% 0.04%, 0.05%, and 0.06%) of maleic hydrazide (MH) were given at seed level. The abnormalities noticed were both physiological and clastogenic. Fragments, stickiness of chromosomes, univalents, multivalents, and anaphase bridges were the most frequent abnormalities. The decrease in seed germination and pollen fertility was found significant at 0.05 level. Mitotic index reduced gradually as the concentrations of Maleic Hydrazide increased and it was found significant at …

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Synthesis Of New Bengazole Analogues And Their Antimicrobial Activity, Jurgen Krauss, Sandra Kalkbrenner, Angela Schuster, Anne Obainoke, Franz Bracher Jan 2008

Synthesis Of New Bengazole Analogues And Their Antimicrobial Activity, Jurgen Krauss, Sandra Kalkbrenner, Angela Schuster, Anne Obainoke, Franz Bracher

Turkish Journal of Chemistry

Bengazole A and bengazole B are 2 representatives out of a large family of marine metabolites isolated from a Jaspis sponge and display potent anti-fungal and anthelmintic activity as well as modest cytotoxic activity. This paper reports a short and efficient synthesis and microbiological evaluation of some new bengazole analogues.

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Synthesis, Spectroscopic And Biological Investigation Of Cyclic Octapeptide: Cherimolacyclopeptide G, Rajiv Dahiya Jan 2008

Synthesis, Spectroscopic And Biological Investigation Of Cyclic Octapeptide: Cherimolacyclopeptide G, Rajiv Dahiya

Turkish Journal of Chemistry

A natural cyclic octapeptide cherimolacyclopeptide G (8) was synthesized by coupling of tetrapeptide units Boc-Gly-Ala-Val-Pro-OMe (5) and Boc-Ile-Tyr-Ala-Pro-OMe (6) after proper deprotection at carboxyl and amino terminals followed by cyclization of the linear peptide segment. The structure was elucidated by IR, ^1H-NMR, ^{13}C-NMR, FAB MS spectral data, and elemental analyses. The newly synthesized cyclopeptide was also evaluated for its antimicrobial, anthelmintic, and cytotoxic activities and found to exhibit potent anthelmintic and cytotoxic activity against earthworms, Megascoplex konkanensis, Pontoscotex corethruses, and Eudrilus species, and Dalton's lymphoma ascites (DLA) and Ehrlich's ascites carcinoma (EAC) cell lines. In addition, compound 8 possessed moderate …

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Characterization And Antimicrobial Activity Of Organotin(Iv) Complexes Of 2-[(2´,6´-Diethylphenylamido)]Benzoates And 3-[(2´,6´-Diethylphenylamido)]Propanoates, Saira Shahzadi, Khadija Shahid, Saqib Ali, Mohammad Bakhtiar Jan 2008

Characterization And Antimicrobial Activity Of Organotin(Iv) Complexes Of 2-[(2´,6´-Diethylphenylamido)]Benzoates And 3-[(2´,6´-Diethylphenylamido)]Propanoates, Saira Shahzadi, Khadija Shahid, Saqib Ali, Mohammad Bakhtiar

Turkish Journal of Chemistry

New organotin(IV) complexes with 2-[(2',6'-diethylphenylamido)] benzoic acid (HL^1) and 3-[(2',6' -diethylphenylamido)]propanoic acid (HL^2) were synthesized by the reaction of di- and triorganotin salts in the presence of triethylamine as base or dioctyltin oxide using a Dean and Stark trap for the removal of azeotropic water. All complexes were characterized by elemental analysis, IR, NMR, and mass spectral studies, and proof that tin-ligand coordination involves only the carboxylate group and complexes show hexa-coordinated geometry in solid state. Multinuclear NMR data show that triorganotin complexes exhibit 4-coordinated geometry while diorganotin complexes show a coordination number greater than 4, probably 5 or 6 …

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Chemical Constituents And Biological Activity Of Three Tanzanian Wild Mushroom Species, L.D. Baraza, C. C. Joseph, M. J. Moshi, M. H. H. Nkunya Dec 2007

Chemical Constituents And Biological Activity Of Three Tanzanian Wild Mushroom Species, L.D. Baraza, C. C. Joseph, M. J. Moshi, M. H. H. Nkunya

Tanzania Journal of Science

The three Tanzanian wild mushroom species Termitomyces letestui, Lactarius edulis and Agaricus sp. aff. arvensis yielded ergosterol, 5,8-peroxyergosterol and ergosta-5,22-dien-3β-ol, and a mixture of ergosterol, ergosta-7,22-dien-3β-ol and ergosta-7-en-3β-ol whose composition was deduced from gas chromatography/mass spectroscopic (GC/MS) analysis of the trimethysilylated mixture. GC/MS analysis of the lipid fraction from T. letestu revealed the presence of linoleic (C18:2), stearic (C18:0), oleic (C18:1), palmitic (C16:0), pentadecanoic (C15:0) and myristic (C14:0) acid. Polar T. letestui and L. edulis fractions yielded α,α,1'trehalose andmannitol. Some of the crude extracts from the three mushroom species showed mild antimicrobial, mosquito larvicidal and cytotoxic activities. The chemical composition …

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Pyridinium Bisretinoids: Synthesis And Photoactivated Cytotoxicity, Junping Gao Dec 2007

Pyridinium Bisretinoids: Synthesis And Photoactivated Cytotoxicity, Junping Gao

Theses and Dissertations

This thesis discusses pyridinium bisretinoid compounds (PBRs), which were prepared for two purposes: 1) to use them as standards for detection of novel fluorophores in human RPE cells, which may be involved in age-related macular degeneration (AMD), and 2) to use them in the development of a targeted and triggered drug delivery system for cancer therapy. We prepared a selection of PBRs using a one-pot biomimetic method; synthesis, mechanisms for formation, and characterization of these compounds is described. We also explored the photoreactivity of three novel PBR compounds and found that these PBRs form oxidation products under blue-light irradiation. The …

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Vitamin E (Tocotrienols) And Prostate Cancer: A Proteomics Approach., Christian Mbangha Muenyi Aug 2007

Vitamin E (Tocotrienols) And Prostate Cancer: A Proteomics Approach., Christian Mbangha Muenyi

Electronic Theses and Dissertations

Proteomics is the large scale study of proteins in cells or organisms. The purpose of this study was to characterize the proteomic alterations occurring in a prostate cancer (LNCaP) cell line after treatment with delta-tocotrienol (a form of vitamin E not very prevalent from most dietary sources). We found that both gamma- and delta-tocotrienols induced time and concentration dependent growth inhibition and programmed cell dead (apoptosis) in LNCaP cells. Secondly, we used two-dimensional gel electrophoresis (2-DE) to characterize changes in protein expression levels associated with this treatment. Our results show that a specific set of proteins are regulated at both …

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Cytotoxicity Of Novel Cisplatin Analogs In Lung, Prostate And Breast Cancer Cells, Van Vo Jan 2007

Cytotoxicity Of Novel Cisplatin Analogs In Lung, Prostate And Breast Cancer Cells, Van Vo

UNLV Retrospective Theses & Dissertations

The antitumor agent that is most prescribed for the treatment of cancer is cis-diamminedichloroplatinum (II) (cisplatin), but its application is limited due to toxic side effects; especially nephrotoxicity. Due to these limitations, numerous analogs have been developed. The cytotoxicity of five novel cisplatin analogs, dichloro(4,4'-diethyl-2,2'-bipyridine)platinum, dichloro(4,4'-dipropyl-2,2'-bipyridine)platinum, dichloro(4,4'-di-t-butyl-2,2'-bipyridine)platinum, dichloro(4,4'-dimethoxy-2,2'-bipyridine)platinum and dichloro[4,4'-bis(3-methoxypropyl)-2,2'-bipyridine]platinum, in lung (A549), prostate (DU-145) and five breast (MCF-7, MDA-MB-435, MDA-MB-231, DC4 and DB46) cancer cell lines were investigated. Clonogenic survivals demonstrated that these novel analogs were 47 to 242 times more lethal than cisplatin and killed cells predominately by apoptosis. The analogs did not typically cause a block in …

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In Vitro Toxicity Evaluation Of Single Walled Carbon Nanotubes On Human A549 Lung Cells, Maria Davoren, Eva Herzog, Alan Casey, Benjamin Cottineau, Gordon Chambers, Hugh Byrne, Fiona Lyng Jan 2007

In Vitro Toxicity Evaluation Of Single Walled Carbon Nanotubes On Human A549 Lung Cells, Maria Davoren, Eva Herzog, Alan Casey, Benjamin Cottineau, Gordon Chambers, Hugh Byrne, Fiona Lyng

Articles

This paper describes the in vitro cytotoxicity assessment of single walled carbon nanotubes (SWCNT) on A549 cells, a human lung cell line. Cellular viability was determined using the alamar blue (AB), neutral red (NR) and MTT assays, which evaluated metabolic, lyso-somal and mitochondrial activity respectively. In addition, the total protein content of the cells was measured using the coomassie bril-liant (CB) blue assay. Supernatants were also assayed for Adenylate Kinase (AK) release and Interleukin 8 (IL-8) which indicated a loss of cell membrane integrity and an in Xammation response respectively.

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A New Approach To Furan-Containing Macrolactones, Christoph Schmidt, Ruth Messmer, Franz Bracher, Jurgen Krauss Jan 2007

A New Approach To Furan-Containing Macrolactones, Christoph Schmidt, Ruth Messmer, Franz Bracher, Jurgen Krauss

Turkish Journal of Chemistry

Furan and tetrahydrofuran heterocycles are part of many natural products, like the Galerucella pheromone, furano epothilones, bipinnatin, and amphidinolides. This paper describes a short and efficient synthetic approach to furan-containing macrolactones in 4 steps, including as key steps a Sonogashira reaction and an olefin metathesis. The resulting compounds were tested at the National Cancer Institute for their cytotoxicity, but did not exhibit significant cytotoxicity in the human tumour cell line screen.

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Antibacterial, Antifungal And Cytotoxic Activities Of Tuberous Roots Of Amorphophallus Campanulatus, Alam Khan, Moizur Rahman, Shariful Islam Jan 2007

Antibacterial, Antifungal And Cytotoxic Activities Of Tuberous Roots Of Amorphophallus Campanulatus, Alam Khan, Moizur Rahman, Shariful Islam

Turkish Journal of Biology

Antibacterial, antifungal and cytotoxic activities of ethanol extract of tuberous roots of Amorphophallus campanulatus were studied. Disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Cytotoxicity was determined against brine shrimp nauplii. In addition, minimum inhibitory concentration (MIC) was determined using serial dilution technique to determine antibacterial potency. The extract showed significant antibacterial activities against four gram-positive bacteria (Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, Streptococcus b-haemolyticus) and six gram-negative bacteria (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella flexneri, Pseudomonas aeruginosa, Salmonella typhi). The MIC values against these bacteria ranged from 16 to 128 µg/ml. The antifungal …

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A New Approach To The Toxicity Testing Of Carbon-Based Nanomaterials: The Clonogenic Assay, Eva Herzog, Alan Casey, Fiona Lyng, Gordon Chambers, Hugh Byrne, Maria Davoren Jan 2007

A New Approach To The Toxicity Testing Of Carbon-Based Nanomaterials: The Clonogenic Assay, Eva Herzog, Alan Casey, Fiona Lyng, Gordon Chambers, Hugh Byrne, Maria Davoren

Articles

The cellular toxicity of three types of carbon nanoparticles, namely HiPco® single-walled carbon nanotubes (SWCNT), arc dischargeSWCNTand Printex 90 carbon black nanoparticles,was studied on three different cell models including the human alveolar carcinoma epithelial cell line (A549), the normal human bronchial epithelial cell line (BEAS-2B) and the human keratinocyte cell line (HaCaT) using the clonogenic assay. Carbon nanomaterials are known to interact with colorimetric indicator dyes frequently used in cytotoxicity assays. By employing the clonogenic assay, any such interactions could be avoided, allowing a more reliable method for the in vitro toxicity assessment of carbon-based nanoparticles. It could be shown …

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Signaling In Natural Killer Cells: Ship, 2b4 And The Kinome, Joseph A. Wahle Jan 2007

Signaling In Natural Killer Cells: Ship, 2b4 And The Kinome, Joseph A. Wahle

USF Tampa Graduate Theses and Dissertations

The NK cell is a large granular lymphocyte that plays a key role in protecting the body against numerous pathogens including parasites, intracellular bacteria, viral infections, as well as showing anti-tumor activity and playing a role in the rejection of allogeneic BM. Unlike other lymphocytic cell types, that utilize rearranging receptors, NK cells are regulated by a complex array of germ line encoded activating and inhibitory receptors. NK cells are often described as a front line or rapid defense given their response to stimuli can be immediate, although they also maintain functions that extend their role well into the adaptive …

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Cellular Mechanism Of Arglabin-Dimethylaminohydrochloride Cytotoxicity, Xiaofei Qin Jul 2006

Cellular Mechanism Of Arglabin-Dimethylaminohydrochloride Cytotoxicity, Xiaofei Qin

Theses and Dissertations in Biomedical Sciences

Cancer is the second leading killer in the United States. Anticancer drug development is always based on the understanding of molecular and cellular mechanisms of carcinogenesis, as well as comprehensive knowledge of potential anticancer drugs. Arglabin-dimethylaminohydrochloride (arglabin-DMA) represents one of the new classes of anti-cancer agents that have shown promise in suppressing the growth of various tumor cells. However, the cellular mechanism of arglabin-DMA cytotoxic effects on tumor cells is still unclear. The current study was to determine the farnesyltransferase (FTase) inhibitory activity of arglabin-DMA and to investigate the effects of arglabin-DMA on three proteins: Ras, Rho and cyclin kinase …

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Cytotoxicity And Inhibitory Activity On Hepatitis B Surface Antigen Secretion From Plc/Prf/5 Cells Of Medicinal Plant Extracts, Thaveechai Prommetta, Sattaporn Sirotamarat, Kruavon Balachandra, Ekarin Saifah Jan 2006

Cytotoxicity And Inhibitory Activity On Hepatitis B Surface Antigen Secretion From Plc/Prf/5 Cells Of Medicinal Plant Extracts, Thaveechai Prommetta, Sattaporn Sirotamarat, Kruavon Balachandra, Ekarin Saifah

The Thai Journal of Pharmaceutical Sciences

Ethanolic extracts of three medicinal plants including Derris scandens, Rhinacanthus nasutus and Santalum album were investb'ated for cytotoxicity by MTT assay and inhibition of hepatitis B surface antigen secretion from PLC/PRF/5 cells by ELISA. Results...

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Chemical Investigation Of Two Antarctic Invertebrates, Synoicum Adareanum (Chordata: Ascidiaceae: Enterogona:Polyclinidae) And AustrodorisKergulenensis (Molusca: Gastropoda:Nudibranchia: Dorididae), Thushara Kelum Kaviraj Diyabalanage Jan 2006

Chemical Investigation Of Two Antarctic Invertebrates, Synoicum Adareanum (Chordata: Ascidiaceae: Enterogona:Polyclinidae) And AustrodorisKergulenensis (Molusca: Gastropoda:Nudibranchia: Dorididae), Thushara Kelum Kaviraj Diyabalanage

USF Tampa Graduate Theses and Dissertations

Synoicum adareanum is a colonial tunicate commonly found on the benthos around Palmer Station on Anvers Island, Antarctica. A comprehensive chemical investigation of the lipophilic extract of the frozen tunicates gave a new series of polyketide macrolides, palmerolides A-E and H. The structure elucidation of these compounds was accomplished by extensive NMR and mass spectral studies.The palmerolides are unusual 20-membered macrolides displaying functionality more commonly found in sponges or cyanobacteria. Palmerolide A, the major member of the group, shows significant and selective in vitro cytotoxicity against melanoma with three orders of greater sensitivity relative to other cell lines tested, in …

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2,6-Hexadecadiynoic Acid And 2,6-Nonadecadiynoic Acid - Novel Synthesized Acetylenic Fatty Acids As Potent Antifungal Agents, Nestor Carballeira, David Sanabria, Clarisa Cruz, Keykavous Parang, Baojie Wan, Scott Franzblau Jan 2006

2,6-Hexadecadiynoic Acid And 2,6-Nonadecadiynoic Acid - Novel Synthesized Acetylenic Fatty Acids As Potent Antifungal Agents, Nestor Carballeira, David Sanabria, Clarisa Cruz, Keykavous Parang, Baojie Wan, Scott Franzblau

Pharmacy Faculty Articles and Research

The hitherto unknown 2,6-hexadecadiynoic acid, 2,6-nonadecadiynoic acid, and 2,9-hexadecadiynoic acid were synthesized in two steps and in 11–18% overall yields starting from either 1,5-hexadiyne or 1,8-nonadiyne. Among all the compounds 2,6-hexadecadiynoic acid displayed the best overall antifungal activity against both the fluconazole resistant Candida albicans strains ATCC 14053 and ATCC 60193 (MIC = 11 μM) and against Cryptococcus neoformans ATCC 66031 (MIC < 5.7 μM). The 2,9-hexadecadiynoic acid did not display any significant cytotoxicity against the fluconazole resistant C. albicans strains, but it showed fungitoxicity against C. neoformans ATCC 66031 with a MIC value of <5.8 μM. Other fatty acids, such as 2-hexadecynoic acid, 5-hexadecynoic acid, 9-hexadecynoic acid, and 6-nonadecynoic acid were also synthesized and their antifungal activities compared. The 2-hexadecynoic acid, a known antifungal fatty acid, exhibited the best antifungal activity (MIC = 9.4 μM) against the fluconazole resistant C. albicans ATCC 14053 strain, but it showed a MIC value of only 100 μM against C. albicans ATCC 60193. The fatty acids 2,6-hexadecadiynoic acid and 2-hexadecynoic acid also displayed a MIC of 140–145 μM towards Mycobacterium tuberculosis H37Rv in Middlebrook 7H12 medium. In conclusion, 2,6-hexadecadiynoic acid exhibited the best fungitoxicity profile compared to other analogues. This diynoic fatty acid has the potential to be further evaluated for use in topical antifungal formulations.

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Chemical Constitutents And Cytotoxicity Of Some Tanzanian Wild Mushrooms, V. Nyigo, L. D. Baraza, M. H. H. Nkunya, S. J. M. Mdachi, C. C. Joseph, A. Waziri Dec 2005

Chemical Constitutents And Cytotoxicity Of Some Tanzanian Wild Mushrooms, V. Nyigo, L. D. Baraza, M. H. H. Nkunya, S. J. M. Mdachi, C. C. Joseph, A. Waziri

Tanzania Journal of Science

The sterol ergosterol and ergosta-4,22-diene-3β,7α-diol, together with 4-hydroxybenzaldehyde were isolated from the mushroom species Polyporus molluscensis, Cantharellus isabelinus, C. symoensii and a Podaxis species. Their structures were established on the basis of spectroscopic data. The ethanol extracts of mushroom samples of Agaricus sp. and Termitomyces letestui also showed cytotoxicity against the brine shrimp larvae.

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Bioassay-Guided Fractionation Of Rubus Suavissimus Leaf Extracts Possessing Nf-Κb Inhibitory Activities And A Separable Cytotoxicity, Dong Liu, Zhanguo Gao, Jian Zhang, Jianping Ye, Zhijun Liu Nov 2005

Bioassay-Guided Fractionation Of Rubus Suavissimus Leaf Extracts Possessing Nf-Κb Inhibitory Activities And A Separable Cytotoxicity, Dong Liu, Zhanguo Gao, Jian Zhang, Jianping Ye, Zhijun Liu

Faculty Publications

Leaf extract of Rubus suavissimus S. Lee (Rosaceae), a perennial woody shrub indigenous to southern China, was tested for its biological activity in the regulation of transcription factor NF-κB (nuclear factor kappa B) A NF-κB luciferase reporter cell line was used in this study. The crude extract inhibited NF-κB at 100-1000 μg/ml concentrations with minor cytotoxicity. This finding prompted two more rounds of fractionation toward separating the active fraction from the cytotoxic fraction. Five fractions were obtained in the first round of fractionation, of which RUS-C20 was found to be the most potent in inhibiting NF-κB without signs of cytotoxicity …

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Cell Toxicology Study Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs)., Clarisse Sornsay Muenyi Aug 2005

Cell Toxicology Study Of Rrr-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (Tpgs)., Clarisse Sornsay Muenyi

Electronic Theses and Dissertations

This research focused on the cytotoxic properties of RRR-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) in transformed and cancerous cell lines. We used RAW264.7 macrophage and prostate cancer (LNCaP) cell lines in this study. TPGS caused cell death and decreased cell viability in a dose and time dependent manner. Cell death was evaluated fluorimetrically by employing the nucleic acid-binding fluorophore; propidium iodide. A colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate cell viability. Cell death can occur through necrosis or apoptosis. Our results suggested that TPGS triggered apoptotic cell death. Induction of apoptosis, as measured by caspase …

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Novel Cisplating Analogs: Synthesis, Characterization And Cytotoxicity In Breast Cells, Kyler Elizabeth Elwell Jan 2005

Novel Cisplating Analogs: Synthesis, Characterization And Cytotoxicity In Breast Cells, Kyler Elizabeth Elwell

UNLV Retrospective Theses & Dissertations

Cis-diamminedichloroplatinum (II) (cisplatin) has been used for three decades as an anti-cancer treatment, but is limited in its therapeutic uses by side effects and cell resistance. In this study, new cisplatin analogues were developed, synthesized, and evaluated for cytotoxicity and cellular mechanisms. Two novel drugs 4,4'-bis(4,4,4-trifluorobutyl)-2,2'-bipyridine-PtCl 2 and 4,4'-bis(4,4,4-trifluorobutyl)-2,2'-bipyridine-Re(CO)3Cl and two previously synthesized drugs 4,4'-dimethyl-2,2'-bipyridine-PtCl 2 and 4,4'-dimethyl-2,2'-bipyridine-Re(CO)3Cl were tested, in breast cancer cell lines MDA-MB-435, MDA-MB-231 and transformed cell lines DC4 and DB46. Cytotoxicity assays found that the rhenium-containing compounds had no effect in the transformed lines and the platinum compounds were more effective than cisplatin at inducing cell …

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Role Of A Cytotoxic-T-Lymphocyte Epitope-Defined, Alternative Gag Open Reading Frame In The Pathogenesis Of A Murine Retrovirus-Induced Immunodeficiency Syndrome, Arti Gaur, William R. Green Nov 2004

Role Of A Cytotoxic-T-Lymphocyte Epitope-Defined, Alternative Gag Open Reading Frame In The Pathogenesis Of A Murine Retrovirus-Induced Immunodeficiency Syndrome, Arti Gaur, William R. Green

Dartmouth Scholarship

LP-BM5 murine leukemia virus-infected C57BL/6 mice develop profound immunodeficiency and B-cell lymphomas. The LP-BM5 complex contains a mixture of defective (BM5def) and replication-competent helper viruses among which BM5def is the primary causative agent of disease. The BM5def primary open reading frame (ORF1) encodes the single gag precursor protein (Pr60gag). Our lab has recently demonstrated that a novel immunodominant cytotoxic-T-lymphocyte (CTL) epitope (SYNTGRFPPL) is expressed from a +1-nucleotide translational open reading frame of BM5def during the course of normal retrovirus expression. The SYNTGRFPPL CTL epitope may be generated from either of two initiation methionines present, ORF2a or ORF2b, located …

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Durable Cytotoxic Immune Responses Against Gp120 Elicited By Recombinant Sv40 Vectors Encoding Hiv-1 Gp120 +/- Il-15., Hayley J Mckee, Patricia Y T'Sao, Maria Vera, Puri Fortes, David S Strayer Aug 2004

Durable Cytotoxic Immune Responses Against Gp120 Elicited By Recombinant Sv40 Vectors Encoding Hiv-1 Gp120 +/- Il-15., Hayley J Mckee, Patricia Y T'Sao, Maria Vera, Puri Fortes, David S Strayer

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: A vaccine that elicits durable, powerful anti-HIV immunity remains an elusive goal. In these studies we tested whether multiple treatments with viral vector-delivered HIV envelope antigen (gp120), with and without IL-15, could help to approach that goal. For this purpose, we used recombinant Tag-deleted SV40-derived vectors (rSV40s), since they do not elicit neutralizing antibody responses, and so can be given multiply without loss of transduction efficiency. METHODS: SV(gp120) carried the coding sequences for HIV-1NL4-3 Env, and SV(mIL-15) carried the cDNA for mouse IL-15. Singly, and in combination, these two vectors were given monthly to BALB/cJ mice. Cytotoxic immunity and …

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Cd4 T Cell Responses To Influenza Infection, Deborah M. Brown, Eulogia Roman, Susan L. Swain Jun 2004

Cd4 T Cell Responses To Influenza Infection, Deborah M. Brown, Eulogia Roman, Susan L. Swain

Nebraska Center for Virology: Faculty Publications

Immune responses to viral infections involve a complex orchestration between innate signals and adaptive responses of specific T and B cells. Anti-viral CD4 cells can direct CD8 responses by secreting a Type 1 panel of cytokines including IFN-γ, IL-2 and TNF- α and can drive B cell production of IgG2a to neutralize infective viral particles. This review will focus specifically on the role of CD4 cells in the immune response to influenza, an acute, localized respiratory viral infection. We suggest that CD4 cells act as direct effectors in protection against influenza, may contribute to immunopathology and generate functionally distinct memory …

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Comparative Cytotoxicity Of Fludarabine In Human Peripheral Blood Mononuclear Cells And In The Promyelocytic Leukemia Cell Line (Hl60), A. Lale Doğan Jan 2004

Comparative Cytotoxicity Of Fludarabine In Human Peripheral Blood Mononuclear Cells And In The Promyelocytic Leukemia Cell Line (Hl60), A. Lale Doğan

Turkish Journal of Medical Sciences

Fludarabine is an inhibitor of DNA/RNA synthesis with antitumor activity on lymphoproliferative malignancies. We investigated the dose-dependent cytotoxic effects of the drug on human peripheral blood mononuclear cells, the HL60 cell line and the differentiated HL60 cells. Dimethylformamide was used for the neutrophilic differentiation of HL60 cells and the MTT assay was used to estimate drug cytotoxicity. Undifferentiated tumor cells had higher proliferation rates compared to the differentiated subline. There was 57% cell death in HL60 cells at a dose of 5 µg/ml of the drug. On the other hand, in differentiated HL60 cells and nonmalignant cells, cell death did …

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Cytotoxicity Of Low Dose Epirubicin-Hci Combined With Lymphokine Activated Killer Cells Against Hepatocellular Carcinoma Cell Line Hepatoma G2, Aysun Özkan, Kayahan Fişkin Jan 2004

Cytotoxicity Of Low Dose Epirubicin-Hci Combined With Lymphokine Activated Killer Cells Against Hepatocellular Carcinoma Cell Line Hepatoma G2, Aysun Özkan, Kayahan Fişkin

Turkish Journal of Medical Sciences

Recent evidance supports the concept that Epirubicin-HCI and Lymphokine-activated killer cells (LAK) cytotoxicity may be mediated by free radical and oxyradical generation. We tested this hypothesis further by exposing Hep G2 cells to Epirubicin-HCI and LAK cells and subsequently monitored cell viability as a measure of cytotoxicity. The cytotoxicity of LAK alone and LAK together with low dose epirubicin-HCI (IC50 1/10) which is measured using the MTT cytotoxicity test on viability of Hep G2 cells and sensitization of target cells to effector cells was investigated. The cytotoxicity of LAK (IC50 value of 5LAK/Hep G2 in 24 hours) and LAK+epirubicin-HCI (IC50 …

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Screening Of Some Rubiaceous Plants For Cytotoxic Activity Against Cervix Carcinoma (Kb-3-1) Cell Line (การคัดกรองความป็นพิษต่อเซลล์มะเร็งปากมดลูกชนิด Kb-3-1 ข..., Patcharawan Tanamatayarat, Porn-Ngarm Limtrakul, Sunee Chunsakaow, Chadarat Duangrat Jan 2003

Screening Of Some Rubiaceous Plants For Cytotoxic Activity Against Cervix Carcinoma (Kb-3-1) Cell Line (การคัดกรองความป็นพิษต่อเซลล์มะเร็งปากมดลูกชนิด Kb-3-1 ข..., Patcharawan Tanamatayarat, Porn-Ngarm Limtrakul, Sunee Chunsakaow, Chadarat Duangrat

The Thai Journal of Pharmaceutical Sciences

Twenty plants in the family Rubiaceae collected from Northern Thailand were extracted and compared for their cytotoxic activities against a human cervix carcinoma cell line (KB-3T). MTT assay was the technique utilized for cell survival determination. Th...

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Purification And Antioxidant Activities Of Soybean Isoflavones, Qiju Wu Jan 2003

Purification And Antioxidant Activities Of Soybean Isoflavones, Qiju Wu

LSU Master's Theses

An efficient procedure was developed and used to extract and purify isoflavones from defatted soy flour. The purity of the final product reached 94%. The antioxidant activities of soybean isoflavones (genistein and daidzein purchased from Sigma Chemical Co, and a mixture of isoflavone glycosides extracted from defatted soy flour) were investigated by a cholesterol oxidation model accelerated by 2,2’-azobis(2-methylpropionamidine) dihydrochloride (AAPH). All components exhibited very high antioxidant activities in the inhibition of cholesterol oxidation. Genistein had higher inhibition to the formation of 5,6beta-epoxycholesterol (5,6b-EP) and 7-ketocholesterol (7-keto), while no significant difference was found between the antioxidant activities of daidzein and …

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Characterization Of Berberine On Human Cancer Cells In Culture, Savio Szeto, Christine Miu Ngan Yow, Ka Wah Fung Jan 2002

Characterization Of Berberine On Human Cancer Cells In Culture, Savio Szeto, Christine Miu Ngan Yow, Ka Wah Fung

Turkish Journal of Medical Sciences

Berberine originates from a Chinese herbal medicine and possesses a wide variety of anti-cancer activities. In this study, the killing effect of berberine on nasopharyngeal carcinoma cells (NPC/HK1) was investigated. The trypan blue exclusion assay was used to assess the cytotoxic effect of berberine in this cell line. Berberine, at 5-200 µM, induced cell death in a dose-dependent manner. Treatment of cells with 200 µM berberine for 5 h yielded a lethal dose of 50% (LD50). The Comet Assay was employed to evaluate the extent of DNA damage and repair after berberine treatment (0 -100 µM). DNA damage was evident …

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