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Phytochemical Characterization, Cytotoxic Effects, And Potential Oxidative Dna Damage-Modulating Activities Of Arum Italicum Extract, Oznour Mourat Moustafa, Hülya Yağar

Turkish Journal of Chemistry

Arum italicum is traditionally utilized in the Thrace region for the treatment of hepatic and dermatological disorders; however, experimental evidence supporting these ethnopharmacological applications remains limited. As oxidative stress-induced DNA damage is an important contributor to the pathogenesis of liver and skin diseases, the aim in the present study was to investigate the ability of A. italicum leaf and tuber extracts to modulate oxidative DNA damage in vitro using nontumorigenic and cancerous hepatic and cutaneous cell models. Phytochemical profiling, antioxidant capacity, and cytotoxic activity were also assessed to support and contextualize the observed DNA damage-related effects.In vitro investigations were conducted …

Chemical Composition, Antifungal Activity, And Cytotoxic Effects Of Indonesian Origanum Vulgare Essential Oil, Ariyani Ariyani, Ismet D. Nasution, Dwi Suryanto, Saharman Gea, Devi R. Syamsir, Hazrina Hazni, Wan H.A.W. Harun, Syarifah N.S.A. Rahman, Khalijah Awang

Journal of Dentomaxillofacial Science

Objective: This study aimed to determine the phytochemical profile, antifungal activity, and cytotoxic effects of Origanum vulgare essential oil cultivated in North Sumatra, Indonesia (IOVEO).
Materials and Methods: Essential oil was extracted from O. vulgare leaves by hydrodistillation and analyzed using gas chromatography flame ionization detection (GCFID) and gas chromatography/mass spectrometry (GC/MS). The antifungal activity of IOVEO against Candida albicans was evaluated using the disc diffusion method. Its cytotoxic effects were assessed using an MTT assay with oral squamous cell carcinoma (OSCC) cell lines and normal human gingival fibroblast cells.
Results: Phytochemical analysis identified 23 compounds, with germacrene D (20.54%), …

Preparation Of Liposomes And Evaluation Of Their Toxic Properties, Aygul M. Yarilkaganova, Oybek N. Ashirоv, Otabek U. Allaev, Nigora А. Tosheva, Shakhnoz S. Azimova

CHEMISTRY AND CHEMICAL ENGINEERING

Liposomes are nanoscale vesicular systems composed of phospholipid bilayers capable of encapsulating both hydrophilic and hydrophobic molecules. The present study investigates the effect of temperature and repeated freeze–thaw (F/T) cycles on liposomal size, polydispersity and evaluates their cytotoxicity on MDSK cell lines using the MTT assay. Liposomes were prepared by the reverse-phase evaporation method and hydrated at different temperatures (30, 40, 50, and 60 °C). The samples were subjected to multiple F/T cycles (1–9×) to study their particle size and polydispersity index (PDI) were analyzed using dynamic light scattering (DLS). Cytotoxicity was tested using the MTT method on MDSK cell …

Synthesis, Spectroscopic Characterization, And Biological Evaluation Of Ag(I), Ni (Ii), And Mn(Ii) Complexes With A Tridentate (N, O,O) Hydroxyazo Ligand, Hussein Hayder Abbood, Azal Shakir Waheeb

Muthanna Medical Journal

Background: HMSA was prepared as a new hydroxyazo ligand with an N, O, O donor arrangement, then used to form Mn (II), Ni (II), and Ag(I) complexes. Objective: The work examined the structural features of HMSA and the prepared metal derivatives and compared their biological responses. Materials and methods: CHNS analysis, FT-IR, UV–Visible and NMR spectroscopy, powder X-ray diffraction (XRD), and FE-SEM were used for characterization. Antibacterial, DPPH antioxidant, and cytotoxicity tests were applied for biological evaluation. Results and conclusion: Spectral changes supported coordination of HMSA through azo nitrogen and phenolic oxygen sites, with a different environment around each studied …

Modified Etoposide Loaded Graphene Oxide Nanoparticles: In-Depth Physical And Biological Evaluation For Lung Cancer Treatment, Umesh Kumar Atneriya, Jitendra Sainy

The Thai Journal of Pharmaceutical Sciences

Background: Lung cancer is approximately 25% of all death cases in any cancer and the death beats the number of deaths reported by colorectal cancer, breast cancer, and prostate cancer combined.
Objectives: This present study was carried out with an objective to investigate the possibility of graphitic carbon toward making a multifunctional anti-lung cancer nano-platform targeting etoposide (ETS) as a model drug.
Materials and Methods: First, the synthesis of graphene oxide (GO) was made by the Hummer method based on graphite flakes and further modified with folic acid (FA) and polyethylene glycol to make FA/GO/ETS. The formation of FA/GO/ETS was …

Nf1 Loss In Estrogen Receptor-Positive Breast Cancer Induces Osteoclast Formation And Immunosuppression To Promote Bone Metastasis, Zifan Zhao, Ze-Yi Zheng, Jonathan T Lei, Matthew J Baik, Yi-Hsuan Wu, Lauren K Somes, Andres F Mosquera Paternina, Omar A Harb, Owen A Chang, Fengshuo Liu, Matthew V Holt, Junkai Wang, Igor Bado, Hai Wang, George Miles, Zbigniew Gugala, Meenakshi Anurag, Ahmed Elkhanany, Yi Li, Valentina Hoyos, Xiang H-F Zhang, Eric C Chang

Faculty, Staff and Students Publications

Estrogen receptor-positive (ER+) breast cancer exhibits a marked propensity for skeletal metastasis; however, the molecular drivers of bone colonization remain incompletely defined. We investigated the tumor suppressor neurofibromin (NF1), a dual repressor for RAS and ER signaling, whose inactivation promotes endocrine therapy (ET) resistance and is associated with inferior relapse-free survival. NF1 copy number loss was detected in 62% of ER+ patients who subsequently developed metastases and was associated with an increased likelihood of bone metastases at initial diagnosis. In mouse xenograft models, NF1-depleted ER+ breast cancer cells demonstrated enhanced dissemination to skeletal sites following surgical resection of primary tumors. …

Comparative Screening Of Cytotoxic Potential Of Berberis Aristata And Senna Alexandrina Extracts, Rida Noor Akhtar, Javaria Zahoor, Rizwan Ullah Khan, Aziz Ullah, Naila Malkani

Journal of Bioresource Management

Medicinal plants provide a safe and efficient source of drugs for cancer treatment. Berberis aristata and Senna alexandrina are traditionally used to treat several ailments because they contain compounds with antimicrobial, antitoxic, anti-inflammatory, and other properties. The objective of the study was to prepare crude ethanolic extracts of B. aristata and S. alexandrina and to determine their cytotoxic potential against cancer cells. Ethanolic extracts were prepared for both plants. Hepatocellular carcinoma cells (HepG2) were used for all assays. The cytotoxicity of extracts was determined by 3‑(4,5‑Dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay using various concentrations of extracts. The obtained sub-lethal concentrations were used …

Comparative Study Of Alginate- And Gum Arabic-Based Liposomal Carboplatin Delivery System, Israa R. Mohammed, Fatimah J. Al-Hasani, Emad S. Al-Hasani

Engineering and Technology Journal

Carboplatin is a common chemotherapeutic drug that has systemic toxicity and low selectivity. This study compares two liposomal drug delivery systems based on natural materials, alginate and gum Arabic, and evaluates the effect of the polymer structure on nanoparticle characteristics, drug release, and anticancer activity. The novelty of this work is the direct link between the polymer architecture and the performance of the drug delivery system on the same platform. Nanoscale formulations were successfully prepared for both formulations. The particle size of gum Arabic was significantly smaller (50 nm) than that of the alginate (170 nm). TEM confirmed the presence …

Synthesis And Characterization Of Organometallic Polymers And Their Cytotoxicity Towards Human Cancer Cell Lines, Paul Peter Slawek

Electronic Theses and Dissertations 2020 - Present

This research aimed to synthesize organometallic polymers from dialkyl (methyl, ethyl, butyl, cyclohexyl, and octyl) and diaryl (phenyl) tin dichlorides and three organic monomers, dipicolinic acid (DPA), chloramphenicol (CH), and isomannide (IS), using interfacial polymerization. Structure-activity relationships were investigated through physicochemical characterization (light-scattering photometry, proton NMR, and IR spectroscopy) and cytotoxicity was evaluated against six human cancer cell lines (AsPC-1, PANC-1, MCF-7, MDA-MB-231, HT-29, PC-3), with cisplatin serving as the reference standard.

Five DPA/tin polymers were synthesized, with IC₅₀ values in the range of 0.60–0.71 μg/mL. Their cytotoxicity was significantly lower than that of cisplatin (0.0023-0.044 μg/mL), suggesting that …

Investigating The Effect Of Functional Group On Folic Acid Conjugation And In Vitro Characteristics Of Lomustine From Silica Nanoparticles, Areen M. Khattabi, Lina M. Ibraheem, Eman M. Migdadi

Baghdad Science Journal

Herein, silica nanoparticles (SNs) with different functional groups (propyl amine, propyl thiol, propyl carboxylic acid and unmodified hydroxyl) were loaded with the anticancer agent lomustine (LMS) and conjugated with Folic Acid (FA) to produce FA-NH₂-SNs, FA-SH-SNs, FA-COOH-SNs, and FA-OH-SNs, respectively. The nanoparticles (NPs) were characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Dynamic Light Scattering (DLS). Encapsulation Efficiency (EE) and drug release rate were measured using UV Spectrophotometer. The in vitro cytotoxicity toward HeLa and VERO cells was assessed using MTT assay. LMS encapsulation efficiency exceeded 40% and FTIR spectra confirmed a successful conjugation with FA. Particle size …

Preparation And Characterization Of Fe2O3 And Fe2O3@Ag/Agcl Core-Shell Nps Using Mint Leaf Extract, And Study Of Their Cytotoxicity And Inhibition Of Breast Cancer Cells, Ruqayah A. Ulwali, Nada K. Abass

Baghdad Science Journal

Iron oxide nanoparticles (Fe₂O₃) and iron oxide core–silver/silver chloride shell nanoparticles (Fe₂O₃@Ag/AgCl) were green-synthesized using mint leaf extract .X-ray diffraction (XRD) results showed that Fe₂O₃ formed in two phases, γ -Fe₂O₃ and α -Fe₂O₃, while Fe₂O₃@Ag/AgCl formed in one phase, α -Fe₂O₃, with crystallite sizes increasing from 1.89 nm for Fe₂O₃ to 22.42 nm for Fe₂O₃@Ag/AgCl. Transmission electron microscopy (TEM) revealed that Fe₂O₃ had …

Analysis Of The Effects Of Astaxanthin On Human Colorectal Cancer Cells, Kara A. Walters

Theses/Capstones/Creative Projects

Astaxanthin (AXT) is an antioxidant carotenoid that is produced by the microalgae Haemococcus pluvialis. This supplement is best known for its potential health benefits including anti-inflammatory, antitumor, immunomodulatory, anticancer and antidiabetic activities. Astaxanthin is marketed as an oral dietary supplement and is widely used in the nutraceutical industry as well as a coloring agent in the food industry. Since Astaxanthin has been increasingly popular as a dietary supplement, cytotoxicity is relevant to consumer exposure. Obtaining the IC50, the concentration that inhibits 50% of cell growth, is important to find how Astaxanthin may interact with cancer, and this can be found …

Pepper Spray Exposure And The Effect On Dna Profiling Of Blood, Brandon Schmakel

Honors Projects

Pepper spray is a common self defense tool used in both law enforcement and civilian settings, with the goal of incapacitating the sprayed individual. In real casework where pepper spray was used, a red-brown stain which tested positive presumptively for blood provided no DNA profile after further testing six months later. A previous graduate student, Emma Crawn, attempted to emulate these casework conditions and instead achieved a DNA profile with slight indications of inhibition or degradation. Further analysis of active pepper spray ingredients such as Oleoresin Capsicum (OC) and Chloroacetophenone (CN) reveals that there are observed reactions, such as oxidation, …

Cytotoxic Effect Of Crab Shell Extracts On Different Tumor Cell Lines, Ahmed M. Al-Shammari, Nahi Y. Yaseen, Shareef Al-Alwaji, Khansaa Raad, Shiamaa Salman Dawood

Al-Qadisiyah Medical Journal

Crab, a kind of marine animal that has a unique status in evolution, has many bioactive substances with special functions. In the present study, we investigated the antitumor effects of four different extracts derived from crab shell (aqueous and methanolic extracts of burned and normal crab shell powder). Aqueous and methanolic extracts of burned crab shell powder were shown to be cytotoxic and inhibit the in vitro proliferation of human laryngeal carcinoma tumor cell lines (Hep-2) and human Rhabdomyosarcoma cell line (RD) and one murine mammary adenocarcinoma tumor cell line (AMN3), in dose and time dependant manner. These results suggest …

The Factors Affecting Anticancer, Antibacterial, And Photocatalytic Applications Of Zno Nanocomposite Synthesized By Plal: A Mini Review, Huda Al-Attar, Maeda Mohammad, Basma Bedair

Engineering and Technology Journal

Fresh water is essential for life to continue on Earth. The accelerated growth of industrialization and globalization is to blame for the current apex of environmental problems. Heavy metals and organic pollutants discharged from industrial waste are extremely harmful at relatively minimal concentrations when tolerance levels are exceeded. They will lead to several diseases in humans. One method for treating wastewater is semiconductor photocatalysis. It depends mainly on the oxidation-reduction mechanism by holes and electrons, respectively, that are generated by the exposure of the semiconductor oxide into a source of photons. Pulsed laser ablation in liquid (PLAL) has attracted significant …

Biological Evaluation Of Novel 6,9-Disubstituted Purine Analogues In High-Grade Serous Ovarian Cancer Cell Lines, Duygu Altiparmak, Deren Demi̇rel Yavuz, Pinar Kul Karadeni̇zli̇, İrem Durmaz Şahi̇n, Meral Tunçbi̇lek

Turkish Journal of Biology

Background/aim: High-grade serous ovarian cancer (HGSOC) remains one of the most aggressive forms of ovarian malignancy and frequently shows resistance to conventional therapies. This study aimed to synthesize a novel series of purine analogues, 6-[(4-substituted benzyl amine)/(4-substituted aniline)]-9-cyclopentyl purines, and evaluate their anticancer efficacy against HGSOC cell lines.

Materials and methods: We assessed the biological effects of the synthesized purine analogues on the OVCAR3, OVSAHO, and KURAMOCHI HGSOC cell lines using the sulforhodamine B assay. To investigate the mechanism of action, we conducted flow cytometry and western blot analyses, focusing on DNA replication and apoptosis.

Results: Among the tested compounds, …

Developing Liposomal And Ethosomal Formulations With Potent Antioxidant And Cytotoxic Effects: Unleashing The Power Of Achillea Millefolium L., Esra Akkol, Sena Akçakaya Mutlu, Gökçe Şeker Karatoprak, Çi̇ğdem Yücel, Selen İlgün, Esra Köngül Şafak, Murat Koç

Turkish Journal of Medical Sciences

Background/aim: Achillea millefolium L., a plant rich in phenolics and flavonoids, has been shown to possess strong antioxidant and cytotoxic properties. However, no pharmaceutical formulations have yet been developed for this plant. This study aimed to create and characterize an A. millefolium extract with high antioxidant and cytotoxic activities.

Materials and methods: A total of 10 extracts were prepared from the plant’s aerial parts using ethanol concentrations ranging from 10% to 99.9%. The DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging tests were used to assess the antioxidant properties of the extracts. Cytotoxic effects were assessed using the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium …

Comparative Genomics Across Ganoderma Species To Identify Secondary Metabolite Clusters With Cytotoxic Properties, Fatima Muhammad, Maya Pasapuleti, Navya Mysore, Emily Abraham, Rajendra P. Pangeni, Braham Dhillon, Navi Gill Dhillon

Bioinformatics

Fungi in the genus Ganoderma have been widely studied in Chinese culture for their medicinal properties and promoting overall health and longevity. Our prior work on eight Ganoderma species showed that they contain Secondary Metabolite Clusters (SMCs) that code for multiple terpenes, betalactones, and ribosomally synthesized and post-translationally modified peptides (RiPPs). These compounds are well known for their cytotoxic and anticancer potential. Using the bioinformatics platform antiSMASH1, we previously identified and annotated the specific SMCs within these genomes that are predicted to produce such bioactive metabolites. To test the results obtained from the bioinformatics analysis, fungal extracts were prepared from …

Biocompatibility And Cytotoxic Effects Of Myofunctional Appliance Materials On Human Periodontal Ligament Fibroblasts, Tsui-Hsien Huang, Tso-Hsun Yang, Chuan-Yi Kao, Chun-Te Ho, Peerapong Santiwong, Chia-Tze Kao

Journal of Dental Sciences

Background/purpose Prefabricated myofunctional devices are widely used in children's dentistry and early orthodontics. This study investigated the effects of materials used in myofunctional appliances on the viability of human periodontal ligament fibroblasts (HPLFs), inflammatory responses, and bone remodeling under simulated oral conditions. The focus was on biocompatibility and cytotoxicity to ensure safety in clinical applications. Materials and methods Four materials—EF Line (EF), ProOrtho (PO), Myobrace (MB), and Invisalign (IV)—were tested under conditions with and without artificial saliva (AS). HPLFs were cultured and exposed to eluates from these materials for 24, 48, and 72 h. Cell viability was measured using the …

Evaluating Thymol Vapor For Biofilm Removal And Biocompatibility In Curved Root Canal Models In Vitro, Maria Paulene Dee Manuel, Yin-Hwa Shih, Shih-Min Hsia, Tong-Hong Wang, Yu-Hsin Tseng, Ming-Gene Tu, Tzong-Ming Shieh

Journal of Dental Sciences

Background/purpose Conventional irrigants such as chlorhexidine and sodium hypochlorite have strong antimicrobial properties but high cytotoxicity, limiting their use in regenerative endodontics. We hypothesized that thymol vapor could provide effective antibacterial activity with lower cytotoxicity. Materials and methods The antimicrobial activity of thymol in both liquid and vapor phases was tested against Enterococcus faecalis, Streptococcus mutans, and Aggregatibacter actinomycetemcomitans using a resin block model simulating curved root canals. The effect of thymol vapor, alone or with mechanical instrumentation, was tested on early-stage biofilms removal. Cytotoxicity was assessed using MTT assays in L-929 fibroblasts and MG-63 osteoblast-like cells, and pro-inflammatory cytokine …

Evaluation Of The Cytotoxicity And Antimicrobial Activity Of Citrus Aurantifolia Extract, Hanan Adnan Shaker Al-Naymi, Sura Mouaid Abbas, Noor Adnan Shaker Al-Naymi, Ayat Majeed Zeadan, Zinah Ameer Abbood

Baghdad Science Journal

The antimicrobial activity of Citrus aqueous extracts was assessed with concentration (100, 80, 60, and 40 μg/ml) using the well diffusion method against Gram-positive, Gram-negative bacteria and yeast that were obtained from urine, vaginal swabs, seminal fluid, sputum, stool, and throat swabs diagnosed by Vitek 2 Compact System. The cytotoxic effect was investigated on tumor hepatic cell line HepG2, Colon cancer CaCo-2 and normal cell line HdFn. The Lethal dose (LD50) for C. aurantifolia was studied on rats. Chemical screening revealed that the aqueous extract contained phenols, tannins, glycosides, saponins, resins, alkaloids, coumarins, and flavones. That exhibits inhibitory activity …

The Cytotoxicity And Anti-Inflammatory Properties Of 1’S-1’-Acetoxychavicol Acetate (Aca) On Oral Cells, Nur Umairah Atiqah Sabri, Nor Syazwani Muhammad Zahidan, Zulfahmi Said, Siti Noor Adnalizawati Adnan, Normaliza Ab Malik

Journal of Dentistry Indonesia

Objective: Mouthwash is widely used to maintain oral health by reducing the prevalence of pathogenic microbiomes. Nonetheless, chlorhexidine, a common active ingredient, is associated with side effects including teeth staining, mucosal peeling, mouth and tongue numbness. This has prompted interest in natural alternatives. This study aimed to identify the cytotoxicity and anti-inflammatory properties of 1’S-1’-acetoxychavicol acetate (ACA) isolated from Alpinia conchigera rhizome against human primary gingival fibroblast and keratinocyte (HPGF and HPGK). Methods: Cytotoxicity of ACA (1.57 mg/mL) against HPGF and HPGK was assessed using the MTT assay. Meanwhile, the anti-inflammatory property of ACA was analyzed based on …

Comparative Analysis Of The Effects Of Peppermint Oil On Colorectal Cancer Cells And Human Fibroblasts, Allen W. Mccumbers

Theses/Capstones/Creative Projects

This study contrasted the effects of Peppermint Oil on two cell lines, HT29s (colorectal adenocarcinoma cells) and HFFs (Human Foreskin Fibroblasts). Cytotoxic as well as genomic effects were compared between the cell lines. Trypan Blue, Alamar Blue, and qPCR assays were used to study these effects. Both cell lines were treated with a range of Peppermint Oil concentrations, and it was determined that there is a dose-dependent factor associated with cytotoxicity. To confirm results, Alamar and Trypan Blue assays were repeated at 300 and 500 cells/mL respectively. Effects were confirmed, and results were averaged and plotted. Alamar Blue revealed that …

Cytotoxicity And Genotoxicity Of Glyphosate And Roundup Quickpro On Chinese Hamster Ovary Cells, Tasnimul Ferdous, Qingfang He, Wen Zhang

Civil Engineering Faculty Publications and Presentations

Glyphosate and Roundup products have become the most used herbicide worldwide. However, previous bans in Europe and recent reversal have caused confusion over their safety. This study aims to investigate the cytotoxic and genotoxic effects of Roundup QuickPro and its main ingredient glyphosate using Chinese hamster ovary (CHO) cells. Cytotoxicity was calculated by measuring CHO cell viability with 72 h of exposure to each toxicant. Roundup QuickPro exhibited higher cytotoxicity with LC50 of 511.2 mu g/mL than glyphosate's LC50 of 3389 mu g/mL. The recommended application concentration (11,233.7 mu g/mL) of Roundup QuickPro is much higher than the measured LC50, …

Cytotoxic And Genotoxic Effects Of Minor Temperature Changes And Zinc Oxide Nanoparticles Synthesized Using Plantago Major On Oncorhynchus Mykiss Gonadal Cells, Semra Çi̇çek, Sevda Işik

Turkish Journal of Zoology

Temperature changes and nanoparticle exposure have emerged as critical environmental stressors with potential cytotoxic and genotoxic effects on aquatic species. This study investigates the impact of zinc oxide nanoparticles (ZnO NPs), synthesized using Plantago major leaves, on Oncorhynchus mykiss gonadal (RTG-2) cells under different temperature conditions (23 °C, 24 °C, and 25 °C). RTG-2 cells were exposed to hexagonal ZnO NPs (≤100 nm) at concentrations of 6.25, 12.5, 25, 50, 75, and 100 μg/mL for 24 and 48 h. Cytotoxicity was assessed using the sulforhodamine B assay, and genotoxicity was evaluated via the comet assay. Results demonstrated that both temperature …

Exposure To Zinc Oxide Nanoparticles Reveals A Death Resistance Mechanism In Dictyostelium Discoideum, Zoya Nadeem Faruqui

Theses and Dissertations

Zinc oxide nanoparticles (ZnO NPs) are very versatile due to their multifunctional properties including unique physicochemical and antimicrobial characteristics. They are widely utilized across various fields such as biomedical, industrial fields, environmental remediation, and agriculture. Therefore, their cytotoxic effects on biological systems necessitate a deeper understanding of the cellular responses to nanoparticle exposure. This project primarily investigates the impact of ZnO NPs on the model organism Dictyostelium discoideum AX4 (social amoeba) which has not been examined before unlike a vast variety of organisms in the literature. The cytotoxic effects of ZnO NPs on Dictyostelium discoideum AX4 cells are compared with …

Specificity Of Interaction Of Antimicrobial Peptide With Cell Membrane, Srishhti Kumar Jha

Pharmaceutical Sciences (MS) Theses

Antimicrobial resistance (AMR) is an increasing global health threat due to its rising incidence worldwide. Resistant microbial infections have become difficult to treat and pose serious risks to patients. In 2019, AMR was responsible for approximately 1.27 million deaths globally, with numbers continuing to rise. The World Health Organization (WHO) has highlighted the urgent need for new antibiotics with novel mechanisms of action and low toxicity to combat this issue. Antimicrobial peptides (AMPs) represent a promising solution. These peptides exhibit broad-spectrum antimicrobial activity and rapid killing kinetics, reducing the likelihood of resistance development. AMPs primarily target bacterial cell membranes. Their …

Targeted Delivery Of Rapamycin Via Epidermal Growth Factor Receptors In Pancreatic Cancer Cells Inhibits Cell Proliferation And Induces Apoptosis, Adeolu S. Oluremi, Christofer Baldwin, Nickolas L. Gustavision, Mujeebat Bashiru, Adeniyi Nsiah Oyebade, Noureen Siraj, Raj Raghavendra Rao, N. Ali

Biomedical Engineering Faculty Publications and Presentations

Pancreatic cancer (PC) is aggressive and deadly; most PC cells overexpress an epidermal growth factor receptor (EGFR). EGFR-targeting peptide (GE11) was conjugated to gold nanoparticles (AuNPs) for delivering rapamycin (Rap), an mTOR inhibitor that reduces proliferation and induces apoptosis. Biophysical techniques confirmed the conjugation. Rap loading efficiency was 41.5%, with sustained release at a lower pH. Conjugates showed higher uptake in PANC-1 cells than unmodified AuNPs. It also inhibited proliferation and induced apoptosis via pathways involving reactive oxygen species, mitochondrial damage, and caspase activation. This approach represents a promising nanoparticle-based therapeutic strategy targeting PC cells, utilizing GE11-EGFR interactions to minimize …

Determination Of Phenotypic Alteration Of Arecoline-Induced Buccal Mucosal Fibroblasts An, Ritiha Patil, Alka D. Kale, Deepa R. Mane, Dhanashree Patil

Indian Journal of Health Sciences and Biomedical Research KLEU

Introduction:

Oral cancer is one of the worldwide health problems accounting as the 6

th

common of all malignancies. Majority of the oral cancer develop from premalignant conditions of the oral cavity due to the chronic habit of tobacco chewing and smoking. The prominent cells of the oral mucosa are fibroblasts playing a major role in synthesis of extracellular matrix, wound healing, and wound repair. Arecoline, one of main ingredients of tobacco is considered as a risk factor for the development of oral premalignant lesions and cancer. The arecoline is reported to have both genotoxic and morphological alteration of oral …

Develpoment Of Urchin-Like Gold Nanoparticles For Drug Delivery Of Glioblastoma And The Colorectal Cancer Cells, Joshua Ozougwu, Vinayak Shama, Bilal Javed Dr., Furong Tian Dr.

Level 3

No abstract provided.