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Articles 6091 - 6120 of 14411
Full-Text Articles in Entire DC Network
The Impacts Of Covid-19 On Business Practice: Some Key Insights, Rajeev Sooreea, Brinda Sooreea
The Impacts Of Covid-19 On Business Practice: Some Key Insights, Rajeev Sooreea, Brinda Sooreea
Barowsky School of Business | Faculty Scholarship
This paper provides an overview of some of the key economic impacts of COVID-19 on business practice, especially in the U.S. In particular, we synthesize some of the latest research, findings and developments from various academic literature and business sources to provide a managerial perspective of the effects of this pandemic. In addition, we show some characteristics of the so-called “new normal” and what kind of innovative business opportunities could arise as a result of the fundamental changes in the global economy. We conclude by highlighting how some emerging countries like India could tap into such opportunities despite the dire …
A Survey On Reading Habit Of Law Students In Tamil Nadu During Covid-19: A Case Study, Suresh N, S Thanuskodi
A Survey On Reading Habit Of Law Students In Tamil Nadu During Covid-19: A Case Study, Suresh N, S Thanuskodi
Library Philosophy and Practice (e-journal)
The COVID-19 pandemic in India is a part of a worldwide pandemic, on 24 March 2020, the Government of India has announced a nationwide lockdown. The COVID-19 pandemic has affected the day-to-day activities particularly has created the largest disruption of education systems in India. This study has examined the impact of COVID-19 lockdown on the reading habit of Law Students in Tamil Nadu.A descriptive survey method was adopted andan online web-based questionnaire was used to collect data for the study. The total response collected for the study is 464 and 65.52% of respondents are female. It was found that there …
Covid-19’S Impact On Financial Markets In Spain, Billy Fleurima
Covid-19’S Impact On Financial Markets In Spain, Billy Fleurima
CISLA Senior Integrative Projects
Late December 2019, emerged what would be recognized as the world’s worst global pandemic since the Spanish Flu. In a 23 month period, roughly 4 million people have died. Everyone’s life has now forever been changed as we move to a virtual world. Remote learning and remote work have dominated both 2020 and 2021. Surprisingly, markets in the United States flourished, dominated by a relatively new trend: Special Purpose Acquisition Companies or SPAC. Across the globe, as countries enforce strict lockdown restrictions, major trends start to emerge worldwide such as a global dearth of semiconductor chips, rise in inflation and …
Factors Predicting Intention To Initiate Mental Health Services Among College Students Returning To Campus During Covid-19, Anne Dumadag, Cara Tonn, Hadiza Galadima
Factors Predicting Intention To Initiate Mental Health Services Among College Students Returning To Campus During Covid-19, Anne Dumadag, Cara Tonn, Hadiza Galadima
College of Health Sciences Posters
Introduction: While the impact of transitioning to a virtual learning environment during the COVID-19 pandemic is well-documented, few studies have explored the mental health impact of reopening campus. As a measurement of mental health impact within the context of reopening campus, this study aims to identify factors associated with intention to initiate mental health services (MHS), with a particular focus on race, among college students.
Methods: A cross-sectional survey using stratified sampling was conducted via Qualtrics among students at a Virginia university in January 2022. The survey assessed demographics, online class experiences, past and future use of MHS, and feelings …
Organizational Impact On Healthcare Workers' Moral Injury During Covid-19: A Mixed-Methods Analysis., Katie E Nelson, Ginger C Hanson, Danielle Boyce, Cathaleen D Ley, Deborah Swavely, Michelle Reina, Cynda Hylton Rushton
Organizational Impact On Healthcare Workers' Moral Injury During Covid-19: A Mixed-Methods Analysis., Katie E Nelson, Ginger C Hanson, Danielle Boyce, Cathaleen D Ley, Deborah Swavely, Michelle Reina, Cynda Hylton Rushton
Reading Hospital Nursing Scholarly Activity
OBJECTIVE: The aim of this study was to explore relationships between organizational factors and moral injury among healthcare workers and the impact of perceptions of their leaders and organizations during COVID-19.
BACKGROUND: COVID-19 placed healthcare workers at risk for moral injury, which often involves feeling betrayed by people with authority and can impact workplace culture.
METHODS: Secondary data from a Web-based survey of mid-Atlantic healthcare workers were analyzed using mixed methods. Data were synthesized using the Reina Trust & Betrayal Model.
RESULTS: Fifty-five percent (n = 328/595) of respondents wrote comments. Forty-one percent (n = 134/328) of commenters had moral …
Teacher Preparation Programs And Teacher Candidates Supporting Staffing Needs During Covid-19 - Program Highlights, Prepared To Teach, Bank Street College
Teacher Preparation Programs And Teacher Candidates Supporting Staffing Needs During Covid-19 - Program Highlights, Prepared To Teach, Bank Street College
Prepared to Teach
A compilation of programs from across the Prepared To Teach National Learning Network that have creative staffing models that directly address staffing and substitute teaching shortages.
Covid-19 Impacts Highlight The Need For Holistic Evaluation Of Research In The Hydrologic Sciences, Cuahsi Board Of Directors & Officers
Covid-19 Impacts Highlight The Need For Holistic Evaluation Of Research In The Hydrologic Sciences, Cuahsi Board Of Directors & Officers
School of Natural Resources: Faculty Publications
No abstract provided.
Public Search Interests Related To Covid-19: Insights From Google Search Trends In Bangladesh, M. Ahamad, M. Ahmed, Daniel R. Uden
Public Search Interests Related To Covid-19: Insights From Google Search Trends In Bangladesh, M. Ahamad, M. Ahmed, Daniel R. Uden
School of Natural Resources: Faculty Publications
No abstract provided.
At War With A Silent Killer: An Ipa Study Of The Lived Experiences Of Community Pharmacists In Ireland During Covid-19, Regina Brady, Niamh Imbusch
At War With A Silent Killer: An Ipa Study Of The Lived Experiences Of Community Pharmacists In Ireland During Covid-19, Regina Brady, Niamh Imbusch
Articles
Objectives: The purpose of this research was to give voice to the unique lived experiences of community pharmacists who continued to work in patient-facing roles throughout the COVID-19 pandemic. Research objectives sought to understand how they felt about working during this time including any risks felt, why they choose to continue working, and what the post-COVID future might hold for them and their profession. Method: The study was guided by the principles of traditional interpretative phenomenological analysis (IPA). Primary research was collated using semi-structured interviews of a small, purposively selected homogenous group of pharmacists. The research context was a …
Drawing The Invisible: Comics As A Way Of Depicting Pscyhological Responses To The Pandemic, Edward Whatley
Drawing The Invisible: Comics As A Way Of Depicting Pscyhological Responses To The Pandemic, Edward Whatley
Library Faculty Scholarship
This graphic article explores the sensory world in the context of pandemic-related mental health issues. While comic artists work in abstract and impressionistic styles to varying degrees, most comic art has been representational. While many artists are adept at rendering facial expressions and body language, these are merely outward manifestations of a character’s mood or emotions. This article explores conveying states of mind less literally, using my own psychological reaction to the pandemic as its subject. I employ visual metaphors, icons, symbols, abstraction, and simplification rather than realistic or highly rendered images in the hopes of better relaying thoughts and …
Covid-19 Financial Consequences In United States Hospitals, Hannah T. Osburn, Murat Mert Solakoglu, Gretel Toloza Alvarez
Covid-19 Financial Consequences In United States Hospitals, Hannah T. Osburn, Murat Mert Solakoglu, Gretel Toloza Alvarez
Theses, Dissertations and Capstones
Introduction: Since the wake of the coronavirus pandemic, United States (U.S.) hospitals have struggled to stay financially afloat amid the constant battle with unprecedented circumstances and financial consequences from the pandemic. The purpose of this research was to analyze the financial consequences of COVID-19 for U.S. hospitals to determine the impact on the number of inpatient cases, length of stay (LOS), surgeries performed, and operating expenses. The hypothesis was that U.S. hospitals were financially affected by the consequences of COVID-19, having more inpatient cases, increased LOS, fewer surgeries performed, and higher operating expenses.
Methodology: The methodology for this …
Lineage Abundance Estimation For Sars-Cov-2 In Wastewater Using Transcriptome Quantification Techniques, Jasmijn A. Baaijens, Alessandro Zulli, Isabel M. Ott, Ioanna Nika, Mart J. Van Der Lugt, Mary E. Petrone, Tara Alpert, Joseph R. Fauver, Chaney C. Kalinich, Chantal B. F. Vogels, Mallery I. Breban, Claire Duvallet, Kyle A. Mcelroy, Newsha Ghaeli, Maxim Imakaev, Malaika F. Mckenzie-Bennett, Keith Robison, Alex Plocik, Rebecca Schilling, Martha Pierson, Rebecca Littlefield, Michelle L. Spencer, Birgitte B. Simen, Yale Sars-Cov-2 Genomic Surveillance Initiative, William P. Hanage, Nathan D. Grubaugh, Jordan Peccia, Michael Baym
Lineage Abundance Estimation For Sars-Cov-2 In Wastewater Using Transcriptome Quantification Techniques, Jasmijn A. Baaijens, Alessandro Zulli, Isabel M. Ott, Ioanna Nika, Mart J. Van Der Lugt, Mary E. Petrone, Tara Alpert, Joseph R. Fauver, Chaney C. Kalinich, Chantal B. F. Vogels, Mallery I. Breban, Claire Duvallet, Kyle A. Mcelroy, Newsha Ghaeli, Maxim Imakaev, Malaika F. Mckenzie-Bennett, Keith Robison, Alex Plocik, Rebecca Schilling, Martha Pierson, Rebecca Littlefield, Michelle L. Spencer, Birgitte B. Simen, Yale Sars-Cov-2 Genomic Surveillance Initiative, William P. Hanage, Nathan D. Grubaugh, Jordan Peccia, Michael Baym
Journal Articles: Epidemiology
Effectively monitoring the spread of SARS-CoV-2 mutants is essential to efforts to counter the ongoing pandemic. Predicting lineage abundance from wastewater, however, is technically challenging. We show that by sequencing SARS-CoV-2 RNA in wastewater and applying algorithms initially used for transcriptome quantification, we can estimate lineage abundance in wastewater samples. We find high variability in signal among individual samples, but the overall trends match those observed from sequencing clinical samples. Thus, while clinical sequencing remains a more sensitive technique for population surveillance, wastewater sequencing can be used to monitor trends in mutant prevalence in situations where clinical sequencing is unavailable.
The Effects Of The Covid-19 Pandemic On Postgraduate Endodontic Programs In The United States, Bianca Aboubakare, James Chen, Johnah C. Galicia
The Effects Of The Covid-19 Pandemic On Postgraduate Endodontic Programs In The United States, Bianca Aboubakare, James Chen, Johnah C. Galicia
Orthodontics and Endodontics Theses
In December 2019, the coronavirus disease 2019 (COVID-19) was first identified as an acute infectious disease in Wuhan, China and subsequently led to an ongoing pandemic. At the onset of the pandemic, dental professionals were understood to face the greatest exposure risk to SARS-Cov-2 due to aerosolization of fluids from the oral cavity and respiratory airways . As a result, dental professionals, including academic institutions and their students and residents halted much of their operations to minimize exposure risks and potentially slow the spread of infection to peers and patients alike. Currently, there is little in the literature that describes …
Violence Against Healthcare Workers: An Epidemic Within A Pandemic, Michaela Ramandanes
Violence Against Healthcare Workers: An Epidemic Within A Pandemic, Michaela Ramandanes
College of Health Sciences Posters
Violence and harassment towards healthcare workers has been an underlying problem throughout history. However, with the rapid and evolving onset of the COVID-19 pandemic, the risks that healthcare workers face has been a focal topic of discussion among administration, staff and the public. What was once considered an occupational hazard is now being raised as a public health issue- an epidemic within a pandemic. This literature review seeks to identify the prevalence and causes of violence and harassment towards healthcare workers. Was healthcare violence as prevalent before COVID-19? Has violence increased because patient volume increased? Additionally, this review will determine …
College Adjustment In Ftic Students During Covid-19, Roksan Aydelman
College Adjustment In Ftic Students During Covid-19, Roksan Aydelman
Honors Undergraduate Theses
This study looked at First Time in College (FTIC) students’ overall college adjustment and whether modality of instruction during students’ senior year of high school relates to subsequent college adjustment. Those high school seniors who completed the entire year virtually experienced greater social isolation and restrictions that may have further limited navigation of developmental tasks and experiences that prepare students for college. Due to the novelty of the COVID-19 pandemic, this is the first cohort where such a marked split in high school experiences could impact future college adjustment. College students (N=294) attending a large public university completed the College …
Giving A Lot Of Ourselves: How Mother Leaders In Higher Education Experienced Parenting And Leading During The Covid-19 Pandemic, Laura E Boche
Giving A Lot Of Ourselves: How Mother Leaders In Higher Education Experienced Parenting And Leading During The Covid-19 Pandemic, Laura E Boche
Department of Educational Administration: Faculty Publications
This qualitative interpretative phenomenological analysis explored the lived experience of mother executive administrators in higher education during the COVID-19 pandemic. Utilizing the philosophical underpinnings of the Heideggerian phenomenological approach, the following research question guided this study: What are the lived experiences of mother executive administrators in higher education during the COVID-19 pandemic? Participants included nine self-identified mother executive administrators from one Midwest state at a variety of institution types and locations within the state. Data collection involved two focus groups and individual interviews with all nine participants. After data analysis, three recurrent themes emerged from the data: (1) Burnout and …
Cholinergic Anti-Inflammatory Pathway And Covid-19, Danial Mehranfard, Robert C. Speth
Cholinergic Anti-Inflammatory Pathway And Covid-19, Danial Mehranfard, Robert C. Speth
HPD Articles
The cholinergic anti-inflammatory pathway (CAP) first described by Wang et al, 2003 has contemporary interest arising from the COVID-19 pandemic. While tobacco smoking has been considered an aggravating factor in the severity of COVID-19 infections, it has been suggested by some that the nicotine derived from tobacco could lessen the severity of COVID-19 infections. This spotlight briefly describes the CAP and its potential role as a therapeutic target for the treatment of COVID-19 infections using vagus nerve stimulation or selective alpha7 nicotinic acetylcholine receptor agonists.
Perceived Effects Of Covid-19 On K-5 Educators And Learners, Fairbee Mintz
Perceived Effects Of Covid-19 On K-5 Educators And Learners, Fairbee Mintz
Master of Science in Nursing Theses and Projects
The COVID-19 pandemic disrupted traditional teaching methods at all academic levels bringing about unprecedented changes in the way education was delivered. In-person learning was halted all over the world as educators scrambled to transition to virtual education. The purpose of this Master of Science in Nursing research was to examine the perceived effects of systematic learning changes caused by the COVID-19 pandemic on primary and secondary educators and students as reported by educators. This research will then be applied to implications for future nursing educators and students. Nine K-5 educators were interviewed via Zoom in the spring of 2021 and …
Returning To A New Normal: A Study Examining Student's Perceptions And Experiences Of Campus Reopening During Covid-19, Cara Tonn, Anne Dumadag, Hira Nadeem, Brenda Berumen-Flucker, Priyanka Patel, Hadiza Galadima
Returning To A New Normal: A Study Examining Student's Perceptions And Experiences Of Campus Reopening During Covid-19, Cara Tonn, Anne Dumadag, Hira Nadeem, Brenda Berumen-Flucker, Priyanka Patel, Hadiza Galadima
College of Health Sciences Posters
Background: Existing research on the mental health and academic impact of the COVID-19 pandemic reports adverse effects during the early stages of the pandemic due to the abrupt campus closures. However, with the relaxation of some restrictions, college students are allowed to return to in-person learning amid new policies. This study aims to assess students' perceptions on several topics related to the pandemic during campus reopening.
Methods: A 22-question cross-sectional survey based on stratified sampling was conducted among 1,025 students at Old Dominion University, and responses were collected via QualtricsTM in January 2022. The survey records socio-demographic information, self-reported …
Telehealth Utilization In Response To The Covid-19 Pandemic: Current State Of Medical Provider Training, Ragan Dubose-Morris, Christina Coleman, Sonja I. Ziniel, Dana A. Schinasi, S. David Mcswain
Telehealth Utilization In Response To The Covid-19 Pandemic: Current State Of Medical Provider Training, Ragan Dubose-Morris, Christina Coleman, Sonja I. Ziniel, Dana A. Schinasi, S. David Mcswain
MUSC Faculty Journal Articles
Background: The COVID-19 pandemic accelerated the development of telehealth services and thus the need for telehealth education and training to support rapid implementation at scale. A national survey evaluating the current state of the telehealth landscape was deployed to organizational representatives, and included questions related to education and training. Materials and Methods: In the summer of 2020, 71 survey participants (31.8%) completed an online survey seeking to determine the utilization of telehealth services across institutional types and locations. This included data collected to specifically compare the rates and types of formal telehealth education provided before and during the pandemic. Results: …
Exploring The Effects Of Masks On Student Engagement In Ece And Ese: A Literature Review Of Related Research, Anna M. Frahm
Exploring The Effects Of Masks On Student Engagement In Ece And Ese: A Literature Review Of Related Research, Anna M. Frahm
Honors Undergraduate Theses
During the advancement of COVID-19, safety protocols (including facial masks) were incorporated into public settings. The Centers for Disease Control and Prevention (CDC), due to safety regulations, recommend wearing face masks when in close contact with other people in public environments, such as in a classroom, where social distancing is difficult. Understanding these CDC recommendations is still critical when looking for effective and safe alternative forms of masking for particular industries. Many industries smoothly transitioned to daily use of traditional cloth masks, but other industries (such as education) serving clients with high communicational needs and communication-centered services sought masking alternatives. …
Covid-19: A Review Of Random Testing As An Augmentation Of A Close-Contact Testing Regime In Response To The Covid-19 Pandemic, John Mcdonnell
Covid-19: A Review Of Random Testing As An Augmentation Of A Close-Contact Testing Regime In Response To The Covid-19 Pandemic, John Mcdonnell
Dissertations
The COVID-19 pandemic has presented the world with a huge challenge to balance the health of the population with the scale of stringent response measures. Policy makers need evidence based recommendations to inform their response to the pandemic and the ever changing landscape it creates. Testing and isolation of infected individuals plays a critical role in containing the spread of the disease, but is aimed primarily at detecting symptomatic individuals. However, asymptomatic individuals are much more difficult to detect. This study presents the augmentation of a close contact testing regime with random testing to examine the elect on the number …
Florida State Law Alumni Magazine & Annual Report (2022), Florida State University College Of Law Office Of Development And Alumni Affairs
Florida State Law Alumni Magazine & Annual Report (2022), Florida State University College Of Law Office Of Development And Alumni Affairs
Alumni Newsletter & FSU Law Magazine
No abstract provided.
Covid-19: Views On Seriousness, Handling/Response, And Behaviors Of Undergraduate Students At The University Of Central Florida, Shemuwel H.I Russ
Covid-19: Views On Seriousness, Handling/Response, And Behaviors Of Undergraduate Students At The University Of Central Florida, Shemuwel H.I Russ
Honors Undergraduate Theses
Objective – Views surrounding COVID-19 are divided. The objective of this thesis is to investigate if views surrounding COVID-19 can be inferred based on college-level demographics. I explore the views of undergraduate students at the University of Central Florida.
Methods – A link to the questionnaire created through Qualtrics was sent out to university leaders asking them to distribute it accordingly among their respective undergraduate populations. Students (N=149) responded to questions about issues related to COVID-19, college-level demographics, and political attitudes. The data was analyzed using both descriptive and inferential analysis.
Results – Students still viewed COVID-19 as a serious …
Advancing Harm Reduction Services In The United States: The Untapped Role Of The Americans With Disabilities Act, Valarie K. Blake, Abigail Fletes, Maria Katherine Delos Reyes, John C. Messinger, Leo Beletsky
Advancing Harm Reduction Services In The United States: The Untapped Role Of The Americans With Disabilities Act, Valarie K. Blake, Abigail Fletes, Maria Katherine Delos Reyes, John C. Messinger, Leo Beletsky
Scholarly Works
Now in its third decade, the overdose crisis continues to worsen. Harm reduction strategies, such as syringe service programs (SSPs), are proven, cost-effective responses to this ongoing public health emergency. Despite extensive research demonstrating that the health and social benefits of harm reduction services far outweigh alleged negative externalities, the number and scope of these programs continue to be severely limited. Restrictive zoning and other discriminatory legal measures figure among key barriers to harm reduction service access. The Americans with Disabilities Act (ADA) and Rehabilitation Act (RA) have recently gained prominence in challenging discrimination against people who seek substance use …
Rethinking Constitutionally Impermissible Punishment, Nadia Banteka
Rethinking Constitutionally Impermissible Punishment, Nadia Banteka
Scholarly Publications
Prisons and jails endanger the health and wellbeing of incarcerated individuals and their communities. These facilities are often overcrowded and unsanitary,1 with limited access to medical care, 2 and no basic sanitation and personal hygiene products unless a person can pay the spiked prices of the jail's commissary.3 Public health emergencies compound these dangers. Most recently, the spread of the COVID-19 pandemic created a crisis for people in detention, their families, and the communities surrounding jails and prisons. For over a year, there were no vaccines against COVID-19, new strains of the virus continue to evade vaccine-induced immunity, and there …
Quantifying The Impact Of Immune History And Variant On Sars-Cov-2 Viral Kinetics And Infection Rebound: A Retrospective Cohort Study, James A. Hay, Stephen M. Kissler, Joseph R. Fauver, Christina Mack, Caroline G. Tai, Radhika M. Samant, Sarah Connolly, Deverick J. Anderson, Gaurav Khullar, Matthew Mackay, Miral Patel, Shannan Kelly, April Manhertz, Isaac Eiter, Daisy Salgado, Tim Baker, Ben Howard, Joel T. Dudley, Christopher E. Mason, Manoj Nair, Yaoxing Huang, John Difiori, David D. Ho, Nathan D. Grubaugh, Yonatan H. Grad
Quantifying The Impact Of Immune History And Variant On Sars-Cov-2 Viral Kinetics And Infection Rebound: A Retrospective Cohort Study, James A. Hay, Stephen M. Kissler, Joseph R. Fauver, Christina Mack, Caroline G. Tai, Radhika M. Samant, Sarah Connolly, Deverick J. Anderson, Gaurav Khullar, Matthew Mackay, Miral Patel, Shannan Kelly, April Manhertz, Isaac Eiter, Daisy Salgado, Tim Baker, Ben Howard, Joel T. Dudley, Christopher E. Mason, Manoj Nair, Yaoxing Huang, John Difiori, David D. Ho, Nathan D. Grubaugh, Yonatan H. Grad
Journal Articles: Epidemiology
BACKGROUND: The combined impact of immunity and SARS-CoV-2 variants on viral kinetics during infections has been unclear.
METHODS: We characterized 1,280 infections from the National Basketball Association occupational health cohort identified between June 2020 and January 2022 using serial RT-qPCR testing. Logistic regression and semi-mechanistic viral RNA kinetics models were used to quantify the effect of age, variant, symptom status, infection history, vaccination status and antibody titer to the founder SARS-CoV-2 strain on the duration of potential infectiousness and overall viral kinetics. The frequency of viral rebounds was quantified under multiple cycle threshold (Ct) value-based definitions.
RESULTS: Among individuals detected …
To Mask Or Not To Mask: Debunking The Myths Of Mask-Wearing During Covid-19 Across Cultures, Rong Chen, Bih-Jen Fwu, Tong-Rong Yang, Quang-Anh Ngo Tran
To Mask Or Not To Mask: Debunking The Myths Of Mask-Wearing During Covid-19 Across Cultures, Rong Chen, Bih-Jen Fwu, Tong-Rong Yang, Quang-Anh Ngo Tran
Psychology | Faculty Scholarship
Mask-wearing is the simplest yet most effective preventive behavior during COVID-19. However, it has sparked great controversy, particularly in America. Little is known about what psychosocial factors predict people’s decision to mask. This research challenges three myths about mask-wearing. First, does mask-wearing provide a false sense of security? Second, is knowledge of COVID-19 a more robust predictor than political ideology of mask-wearing behavior? Third, does resistance to masks reflect anti-authoritarianism or a lack of trust in government? With nationally representative samples across two cultures (N = 1,121), findings reveal a significant positive correlation between mask-wearing and other preventive behaviors. …
Insect Cell Expression And Purification Of Recombinant Sars-Cov-2 Spike Proteins That Demonstrate Ace2 Binding, Lucas R. Struble, Audrey L. Smith, William E. Lutz, Gabrielle Grubbs, Satish Sagar, Kenneth W. Bayles, Prakash Radhakrishnan, Surender Khurana, Dalia El-Gamal, Gloria E. O. Borgstahl
Insect Cell Expression And Purification Of Recombinant Sars-Cov-2 Spike Proteins That Demonstrate Ace2 Binding, Lucas R. Struble, Audrey L. Smith, William E. Lutz, Gabrielle Grubbs, Satish Sagar, Kenneth W. Bayles, Prakash Radhakrishnan, Surender Khurana, Dalia El-Gamal, Gloria E. O. Borgstahl
Journal Articles: Pathology and Microbiology
The COVID-19 pandemic caused by SARS-CoV-2 infection has led to socio-economic shutdowns and the loss of over 5 million lives worldwide. There is a need for the identification of therapeutic targets to treat COVID-19. SARS-CoV-2 spike is a target of interest for the development of therapeutic targets. We developed a robust SARS-CoV-2 S spike expression and purification protocol from insect cells and studied four recombinant SARS-CoV-2 spike protein constructs based on the original SARS-CoV-2 sequence using a baculovirus expression system: a spike protein receptor-binding domain that includes the SD1 domain (RBD) coupled to a fluorescent tag (S-RBD-eGFP), spike ectodomain coupled …