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The Effects Of Tail Flukes On The Swimming Pattern Of Atlantic Bottlenose Dolphins, Lauri Leach

Senior Honors Projects

Atlantic bottlenose dolphins are top predators in the wild, due in part because they are excellent swimmers. When dolphins swim through the water, the tail flukes are moved in an up and down motion to propel the animal through the water. They use the pectoral fins for steering and braking, and the dorsal fin for stabilization as they swim. Using this style of swimming, Atlantic bottlenose dolphins can swim up to 25 miles per hour and leap 15 to 20 feet into the air.

During the spring of 2008, I was an intern at the Clearwater Marine Aquarium in Florida. …

Biomersa: The Biology Media Repository With Semantic Augmentation, Adam Cornwell

Theses

With computers now capable of easily handling all kinds of multimedia files in vast quantity, and with the Internet now well-suited to exchange these files, we are faced with the challenge of organizing this data in such a way so as to make the information most useful and accessible. This holds true as well for media pertaining to the field of biology, where multimedia is particularly useful in education, as well as in research. To help address this, a software system with a Web-based interface has been developed for improving the accuracy and specificity of multimedia searching and browsing by …

An Analysis Of The Environmental And Hormonal Effects On The Growth And Development Of The Moss Ceratodon Purpureus, Megan Knight

Undergraduate Honors Thesis Collection

Ceratodon has not been documented in scientific literature as thoroughly as other species of moss such as Funaria or Tortula. Commonly known as fire moss or purple horned moss, Ceratodon is often reddish or yellow-brown and the spore capsules are usually purple (Crum, 1983). A picture of the species growing in its natural habitat is shown in Figure 4. Ceratodon often grows in tufts and is considered a weed, often thriving in polluted or disturbed areas and frequently invading after a fire (Crum 1983). Ceratodon belongs to the class Bryopsida, which also contains the previously mentioned Funaria and Tortula species. …

From Cells To Coastlines: How Can We Use Physiology To Forecast The Impacts Of Climate Change?, Brian Helmuth

Faculty Publications

The interdisciplinary fields of conservation physiology, macrophysiology, and mechanistic ecological forecasting have recently emerged as means of integrating detailed physiological responses to the broader questions of ecological and evolutionary responses to global climate change. Bridging the gap between large-scale records of weather and climate (as measured by remote sensing platforms, buoys and ground-based weather stations) and the physical world as experienced by organisms (niche-level measurements) requires a mechanistic understanding of how ‘environmental signals’ (parameters such as air, surface and water temperature, food availability, water flow) are translated into signals at the scale of the organism or cell (e.g. body temperature, …

Biology And Conservation Of Horseshoe Crabs, John T. Tanacredi, Mark L. Bottom, David R. Smith

School of Marine and Environmental Sciences Faculty and Staff Publications and Presentations

We dedicate this book to Drs. Carl N. Shuster, Jr. and Koichi Sekiguchi for their life-long contributions to the biology and conservation of the magnificent horseshoe crab.

Lifelines Winter 2009, Southern Adventist University

Lifelines - Biology Department Newsletter

The Winter 2009 issue of Lifelines features articles on RAM Clinics, Dr. Earl Aagaard's retirement, the addition of Richard Seidel to the faculty, and a pictorial directory of the 2009 biology and allied health graduates.

Exploring The Relationship Between Sampling Efficiency And Short Range Endemism For Groundwater Fauna In The Pilbara Region, Western Australia, Harley J. Barron, James Cocking, Stefan Eberhard

KIP Articles

No abstract provided.

Annual Report October 1, 2006 - September 30, 2007 Endangered Species Act Section 10(A)1(B) Permit For The Incidental Take Of The Barton Springs Salamander (Eurycea Sosorum)

KIP Data Sets and Technical Reports

Table of Contents Summary of Compliance 2 Summary of Salamander Status/Biological Data 14 Pool Maintenance and Management Activities 21 Barton Springs Improvements Master Plan Short-Term Projects 25 Literature Cited 32 Open Access - Permission by Publisher See Extended description for more information.

Genetic Variation Within The Daphnia Pulex Genome, Abraham Eaton Tucker

Doctoral Dissertations

Genetic variation within the diploid Daphnia pulex genome was examined using a high quality de novo assembly and shotgun reads from two distinct D. pulex clones. Patterns of variation and divergence at single nucleotides were examined in physical and functional regions of the genome using comparative assembly output and available annotations. Additionally, mitochondrial genomes of the same D. pulex clones were assembled and compared for patterns of divergence, and substitutional biases. Intron presence/absence polymorphisms were identified computationally and verified experimentally. Finally, gene duplicate demographics were examined for patterns of divergence and estimates of gene birth rates.

Modeling Congenital Disorders Of Glycosylation In Caenorhabditis Elegans: Genetic Influences And Structural Consequences Of N-Linked Glycosylation, Weston Booth Struwe

Doctoral Dissertations

The attachment of oligosaccharides to the amide nitrogen of asparagine side chains in proteins is a fundamental process occurring in all metazoans. This process, known as N-glycosylation, is complex and is achieved by the precise interactions of various cellular components. The initial stage of N-glycosylation occurs in the endolasmic reticulum and is preserved among eukaryotes. Glycans are further developed in the Golgi and the structural complexity depends greatly on the animal species, tissue and developmental stage. Oligosaccharides are unique biomolecules because unlike DNA or proteins, no primary sequence exists nor is its' synthesis template driven. A major goal of glycobiologist …

Photosynthetic And Oxidative Stress In The Green Alga Dunaliella Tertiolecta: The Effects Of Uv-B And Uv-A Radiation, Priya Sampath Wiley

Doctoral Dissertations

The penetration of ultraviolet-B (UV-B; 290-320 nm) into the biosphere has increased in response to decreased stratospheric ozone. As a consequence, significant attempts have been made to elucidate the effects of UV-B radiation on primary producers such as phytoplankton and plants. Considerably less effort has been devoted to describing the role played by ultraviolet-A (UV-A; 320-400 nm) radiation, which is not attenuated by stratospheric ozone. The present work details the independent and combined effects of UV-B and UV-A radiation on photosynthetic and oxidative stress responses using the unicellular green alga Dunaliella tertiolecta as a model organism. A UV-B spectral profile …

Gonadotropin Releasing Hormone In Agnathans, Scott Ira Kavanaugh

Doctoral Dissertations

Hagfish and lampreys are the only two representatives of Agnathans among extant vertebrates. The regulatory hypothalamic neurohormone, gonadotropin-releasing hormone (GnRH), regulates reproduction in all vertebrates through the hypothalamic-pituitary-gonadal axis. Most vertebrates have at least two forms of GnRH in the brain. In hagfish the primary amino acid structure of GnRH has not been identified as yet, however, indirect methods have shown an immunoreactive GnRH or a GnRH-like peptide in the brain of hagfish. In addition concentrations of brain immunoreactive-GnRH have been correlated with reproductive stages in the Atlantic hagfish ( Myxine glutinosa). Therefore the objective of the first study was …

Tracking Diet And Movement Of Atlantic Bluefin Tuna (Thunnus Thynnus) Using Carbon And Nitrogen Stable Isotopes, John Logan

Doctoral Dissertations

Atlantic bluefin tuna (ABFT; Thunnus thynnus) are important top predators in pelagic ecosystems. Understanding their diet and movements is necessary for proper management, but existing methods are costly and logistically challenging. Naturally occurring stable isotopes have been used to study diet and movement in many aquatic ecosystems and offer a novel approach for studying these large pelagic fishes.

Lipids, which have lower carbon isotope values than protein, can affect isotope analyses of bulk tissue. Both chemical and mathematical corrections were used to address lipid effects on ABFT and the prey. All chemical extraction methods altered nitrogen isotope values. Mathematical corrections …

Acute And Chronic Effects Of Coal Tar And Asphalt Sealants On Salamanders, Thomas Bommarito, Richard Halbrook, Donald W. Sparling

KIP Data Sets and Technical Reports

The Barton Springs salamander (Eurycea sosorum) is a federally endangered species inhabiting pools of the Edwards aquifer in the City of Austin, Texas. The presence of polycyclic aromatic hydrocarbons (PAH) in these pools elicited concern for the conservation of the species because 4 PAHs have acute and chronic effects on aquatic organisms, including amphibians. These PAHs appear to be coming from sealants applied to pavements within the watershed of these ponds. To determine if Barton Springs salamanders could be a risk to these PAHs three laboratory experiments were funded by the Barton Springs Conservation Foundation and conducted by at Southern …

Orientation Of Vernal Pool Amphibians In An Industrial Forest Landscape, Emma Carcagno

Master's Theses and Capstones

Understanding the movement patterns of vernal pool amphibians is a critical aspect of effective conservation and land management. I used clearcutting to manipulate buffer widths at 11 vernal pools within an industrial forest landscape located in Maine. Forested buffers were either 30m or 100m wide, surrounded by 100m wide clearcut. Each pool was encircled with a drift fence and pitfall traps. I captured wood frogs (Lithobates sylvaticus ) and spotted salamanders (Ambystoma maculatum) as they entered and exited pools and documented orientation across three years. Orientation at all pools for both species was non-uniform, differed among pools, between species, and …

Distribution Of Epibionts And Their Effects On The Marine Snail Littorina Littorea In Northern New England, Laura Chelynne Page

Master's Theses and Capstones

Epibiosis, or the growth of one organism on another, is a common life history strategy in marine environments where space is at a premium. Many epibiotic organisms live on or in biotic hard substrates, such as shells. In the spatially competitive rocky intertidal of New England, hard substrate surface area is greatly increased by the presence of the non-native marine periwinkle snail Littorina littorea. The snail's shell can be exploited by epibiotic organisms such as barnacles, bryozoans, and encrusting calcareous algae. However, an in-depth examination of the prevalence and impact of epibionts on Littorina littorea in New England has not …

Experimental Evolution Of Vibrio Fischeri To Squid Symbiosis, Lauren Perry

Master's Theses and Capstones

Background. Co-evolution of Vibrio fischeri strains with Euprymna scolopes has led to isolates that are superior squid colonists [1, 2]. To better understand how V. fischeri adapts to symbiosis with squid we serially transferred a planktonic Hawaiian isolate, H905 [3], and the natural E. scolopes symbiont, ES114, in squid. We characterized derived isolates for colonization ability as well as other phenotypic traits that have been implicated as being important to symbiosis.

Results. We hypothesized that as a result of adaptation to symbiosis H905 would become more phenotypically similar to ES114. We see this trend in luminescence and siderophore production of …

Disruption Of Cytokeratin 18-Containing Intermediate Filaments In Bovine Luteal Cells: Effects On Fas Expression, Progesterone Secretion, And Fasl-Induced Apoptosis, Alice Duncan

Master's Theses and Capstones

In the current study, the possibility that cytokeratin 18 (CK18)---containing intermediate filaments (IFs) protect bovine luteal cells from FasL-induced apoptosis was explored. Bovine corpora lutea (CL) from early and late stages of the luteal phase were collected and prepared for cell culture. The cultures were exposed to culture medium without (control) or with 5mM acrylamide for 4 hr to disrupt CK18 IFs. Subsequently, the cultures were exposed to fresh medium without (control) or with FasL (50ng/mL) for 24 hr to induce apoptosis. Acrylamide disrupted CK18 IFs without affecting microtubules, progesterone secretion (P>0.05), or cell viability (P>0.05). Surface expression …

Frequency Of Reovirus Detection In Biosolids: Comparison Of The Epa Cfr 503 Technique To Integrated Cell Culture - Quantitative Pcr, Elizabeth Gallagher

Master's Theses and Capstones

The public health threat from pathogens creates controversy for the land application of biosolids, a sewage treatment byproduct. Previous work has demonstrated that some enteric viruses are not detected with a plaque assay, the current method for virus detection in biosolids. The Integrated Cell Culture - Quantitative Polymerase Chain Reaction (ICC-qPCR) assay, which combined quantitative PCR with seven days incubation in cell culture, allows for detection of more viruses.

To compare method sensitivities, a biosolid sample was seeded with mammalian orthoreovirus. 3x105 plaque forming units (PFU) per ml were detected by the plaque assay and 108 PFU equivalents per ml …

Runx1 In Primitive Hematopoiesis And Characterization Of Hematopoietic Stem Cells In A Mouse Chronic Inflammatory Arthritis Model, Yunglin Ma

All Theses and Dissertations (ETDs)

Hematopoietic cells are essential for growth and survival throughout adult life. Two different aspects of hematopoiesis are addressed in this dissertation. I. The regulation of primitive hematopoiesis by Runx1 and TGF-beta signaling. Primitive hematopoiesis, occurring exclusively in the yolk sac, is characterized by its transient nature. As the primitive hematopoiesis declines in the yolk sac, definitive hematopoietic progenitors generated in the yolk sac and/or embryo take over in blood cell production. Whether the transition from primitive to definitive hematopoietic program reflects a mere shift in hematopoietic sites or whether it is an actively regulated process is currently unknown. Runx1 is …

Mechanisms Of Feedback In The Visual System, Adam Eggebrecht

All Theses and Dissertations (ETDs)

Feedback is an ubiquitous feature of neural systems though there is little consensus on the roles of mechanisms involved with feedback. We set up an in vivo preparation to study and characterize an accessible and isolated feedback loop within the visual system of the leopard frog, Rana pipiens. We recorded extracellularly within the nucleus isthmi, a nucleus providing direct topographic feedback to the optic tectum, a nucleus that receives the vast majority of retinal output. The optic tectum and nucleus isthmi of the amphibian are homologous structures to the superior colliculus and parabigeminal nucleus in mammals, respectively. We formulated a …

A Quest For Meaning In Spontaneous Brain Activity - From Fmri To Electrophysiology To Complexity Science, Biyu He

All Theses and Dissertations (ETDs)

The brain is not a silent, complex input/output system waiting to be driven by external stimuli; instead, it is a closed, self-referential system operating on its own with sensory information modulating rather than determining its activity. Ongoing spontaneous brain activity costs the majority of the brain's energy budget, maintains the brain's functional architecture, and makes predictions about the environment and the future. I have completed three separate studies on the functional significance and the organization of spontaneous brain activity. The first study showed that strokes disrupt large-scale network coherence in the spontaneous functional magnetic resonance imaging: fMRI) signals, and that …

Coiled-Coil-Mediated Dimerization Is Not Required For Myosin Vi To Stabilize Actin During Spermatid Individualization In Drosophila Melanogaster, Tatsuhiko Nogushi, Deborah J. Frank, Mamiko Isaji, Kathryn G. Miller

Biology Faculty Research

Myosin VI is a pointed-end-directed actin motor that is thought to function as both a transporter of cargoes and an anchor, capable of binding cellular components to actin for long periods. Dimerization via a predicted coiled coil was hypothesized to regulate activity and motor properties. However, the importance of the coiled-coil sequence has not been tested in vivo. We used myosin VI's well-defined role in actin stabilization during Drosophila spermatid individualization to test the importance in vivo of the predicted coiled coil. If myosin VI functions as a dimer, a forced dimer should fully rescue myosin VI loss of function …

The Role Of Astrocyte Activation In Infantile Neuronal Ceroid Lipofuscinosis, Shannon Macauley-Rambach

All Theses and Dissertations (ETDs)

Infantile neuronal ceroid lipofuscinoses: INCLs), or Batten Disease, is an inherited neurodegenerative lysosomal storage disorder affecting the central nervous system: CNS) during infancy or childhood. Hallmark pathological changes include accumulation of autofluorescent material, neuronal loss, cortical thinning, and brain atrophy, which ultimately lead to cognitive deficits, motor dysfunction, seizure activity, and blindness. INCL is the result of mutations in the CLN1 gene leading to a deficiency in the lysosomal enzyme, palmitoyl protein thioesterase 1: PPT1). A mouse model of INCL, the PPT1-deficient: PPT1-/-) mouse, was recently created by a targeted disruption in the CLN1 gene. The phenotype of the PPT1-/- …

Expression Analysis And Stem Cell Engineering, Cara Rieger

All Theses and Dissertations (ETDs)

The overall goal of the thesis was to develop tools to advance Investigations of stem cells in tissue engineering therapeutics for neurodegenerative disease and spinal cord injury. Two tools to characterize cell fate and a tool to separate a subset of neural cells were developed and evaluated. In the first study, a digital PCR technology, called polonies, was applied to measure mRNA from several key stem cell genes in small numbers of ES cells. Due to its properties, we hypothesized that polonies would be uniquely poised to profile stem cells. Polonies were counted for Oct3 in a sample of 10 …

Subunit Compositions Of Arabidopsis Dna-Dependent Rna Polymerases And The Roles Of The Plant-Specific Rna Polymerases Iv And V In Gene Silencing, Thomas Ream

All Theses and Dissertations (ETDs)

In addition to RNA polymerases I, II and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play non-redundant roles in siRNA-directed DNA methylation and gene silencing. Using a combination of affinity purification and protein identification by mass spectrometry, my studies show that Arabidopsis Pol IV and Pol V are composed of subunits that are paralogous or identical to the twelve subunits of Pol II. Four subunits of Pol IV are distinct from their Pol II paralogs, six subunits of Pol V are distinct from …

The Effect Of Particle-Associated Viruses On Disinfection Processes In Water Treatment, Jessica L. Tokson

Master's Theses and Capstones

This research focused on what accelerates or hinders virus-particle association, how the addition of turbidity affects both chlorine and ultraviolet light disinfection, and if sonication can disrupt virus-particle associations exposing viruses to disinfection methods.

A decrease in pH, from 7--4, was found to accelerate association by 2-logs. Calcium increased association by 2.74-logs, and total organic carbon decreased association by 0.36-logs.

An addition of 5 NTU, the maximum turbidity level allowed by USEPA for unfiltered drinking water supplies, affected both chlorine and UV light disinfection showing differences in the rate of kill per dose. The association of viruses and particles can …

Service Learning In A Non-Majors Biology Course Promotes Changes In Students’ Attitudes And Values About The Environment, Alissa Packer

International Journal for the Scholarship of Teaching & Learning

I was interested in whether the inclusion of a service-learning experience would affect students’ attitudes and values about the environment. To investigate this question, I incorporated a service-learning project into the laboratory portion of one of the two sections of my non-majors biology course, and kept the lecture portions of the course identical. Several methods were used to assess the impact of the service-learning experience: the New Ecological Paradigm survey was used to measure changes in students’ attitudes and values about the environment at the beginning and end of the semester, and reflective writing assignments were used throughout the semester …

In Vivo Function Of Otopetrin 1 In The Vestibular Sensory Epithelium, Euysoo Kim

All Theses and Dissertations (ETDs)

Otopetrin family genes encode multi-transmembrane domain proteins with three highly conserved domains. In mice, three Otopetrin paralogues are found. One of its members, Otopetrin 1: Otop1) has been previously shown to be essential for the formation of otoconia in the vestibular system of the inner ear. Otoconia are calcium carbonate biominerals that are required for normal balance and the sensation of linear acceleration with respect to gravity. The mechanism by which OTOP1 mediates otoconia biosynthesis is not known, but the ability of OTOP1 to modulate [Ca2+]i in response to purinergic signals in heterologous systems suggest that OTOP1 may be involved …

Microbial Diversity And Geochemical Energy Sources Of Tutum Bay, Ambitle Island, Papua New Guinea, An Arsenic-Rich, Shallow-Sea Hydrothermal System, Nancy Akerman

All Theses and Dissertations (ETDs)

I investigate the hydrothermal system located in Tutum Bay, Ambitle Island, Papua New Guinea, a shallow-sea system ~5 - 10 meters below sea level that is arsenic-rich. Hydrothermal vents in the bay expel fluids with arsenite: AsIII) concentrations as high as 950 μg/L. To determine the role that Tutum Bay microorganisms might play in mediating As-redox reactions, three approaches were used: analyzing the geochemical environment for energy sources, characterizing the archaeal community composition of the sediments, and conducting culture-dependent As-cycling experiments. The second chapter of this dissertation discusses an energetic study of potential chemolithotrophic metabolic reactions, including As-redox reactions. Results …