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Nf96-251 A Comparative Study Of Fiber Digestion And Subsequent Nutrient Absorption In The Ostrich Versus The Ruminant, Sheila E. Scheideler

University of Nebraska-Lincoln Extension: Historical Materials

This NebFact compares the digestive systems of the ruminant and the ostrich.

Toxicity Of Aspen Wood Leachate To Aquatic Life: Laboratory Studies, Barry R. Taylor, J. Stephen Goudey, N. Bruce Carmichael

Aspen Bibliography

Trembling aspen (Populus tremuloides Michx.), a common hardwood tree throughout Canada, is being harvested at increasing rates for use in paper and building materials. Piles of aspen logs have been observed to produce a dark, watery, acutely toxic leachate. A laboratory study was undertaken to elucidate the nature, strength, and persistence of aspen leachate toxicity and the chemical composition of the leachate. Leaching from aspen chips in the laboratory was rapid, with 1% mass loss in the first 24 h. Another 2 weeks of immersion was necessary to remove all remaining leachable material (3% total)> Fresh aspen leachate derived …

Crow Cries, Erin Hasley, Fleet Library, Special Collections, Jan Baker

Animals

This book was created for Jan Baker's artists' book class.

The Spider And The Fly, Matthew Palin, Fleet Library, Special Collections, Jan Baker

Animals

This book was created for Jan Baker's artists' book class.

Capture And Counteraction: Self- Help By Environmental Zealots (Allen Chair Symposium 1996: The Future Of Environmental And Land-Use Regulation), James E. Krier

Articles

Self-help is a largely neglected topic in American legal studies.1 With the exception of a survey by a group of law students published a dozen years ago,2 there appears to be little, if anything, in our legal literature that confronts the subject in a systematic way.3 This is so, at least, if one defines self-help as I do. To me, the term refers to any act of bypassing the formal legal system in order to get what one wants.

Lady Bug, Ryan Andrew Scro, Fleet Library, Special Collections, Jan Baker

Animals

This book was completed for Jan Baker's artists' book class, Printed Books

Inactivation Of Raf-1 By A Protein-Tyrosine Phosphatase Stimulated By Gtp And Reconstituted By G Subunits, Paul Dent, Dean B. Reardon, Steven L. Wood, Margaret A. Lindorfer, Stephen G. Graber, James C. Garrison, David L. Brautigan, Thomas W. Sturgill

Faculty & Staff Scholarship

No abstract provided.

A Requirement For Bone Morphogenetic Protein-7 During Development Of The Mammalian Kidney And Eye., Andrew T. Dudley, K. M. Lyons, E. J. Robertson

Journal Articles: Genetics, Cell Biology & Anatomy

BMP-7/OP-1, a member of the transforming growth factor-beta (TGF-beta) family of secreted growth factors, is expressed during mouse embryogenesis in a pattern suggesting potential roles in a variety of inductive tissue interactions. The present study demonstrates that mice lacking BMP-7 display severe defects confined to the developing kidney and eye. Surprisingly, the early inductive tissue interactions responsible for establishing both organs appear largely unaffected. However, the absence of BMP-7 disrupts the subsequent cellular interactions required for their continued growth and development. Consequently, homozygous mutant animals exhibit renal dysplasia and anophthalmia at birth. Overall, these findings identify BMP-7 as an essential …

Stimulation Of Alpha 1(I) Procollagen Gene Expression In Nih-3t3 Cells By The Human T Cell Leukemia Virus Type 1 (Htlv-1) Tax Gene., E. Munoz, D. Suri, S. Amini, K. Khalili, Sergio A. Jimenez

Selected Works of Sergio Jiménez, MD, MACR

The mechanisms that regulate the expression of genes encoding extracellular matrix proteins in fibroblasts and other mesenchymal cells have remained elusive. Studies from several laboratories have indicated that Tax, a trans-regulatory protein from the human T cell leukemia virus type I not only augments viral gene expression but also triggers the expression of various cellular genes. Here, we examined the hypothesis that the expression of collagen genes may also be modulated by Tax. NIH-3T3 cells were simultaneously transfected with a Tax expressor plasmid and a chimeric construct containing regulatory sequences (-804 to +42 bp) of the alpha 1(I) procollagen gene …

Survival Of Mouse Pancreatic Islet Allografts In Recipients Treated With Allogeneic Small Lymphocytes And Antibody To Cd40 Ligand., David C. Parker, Dale L. Greiner, Nancy E. Phillips, Michael C. Appel, Alan W. Steele, Fiona H. Durie, Randolph J. Noelle, John P. Mordes, Aldo A. Rossini

Dartmouth Scholarship

Combined treatment with allogeneic small lymphocytes or T-depleted small lymphocytes plus a blocking antibody to CD40 ligand (CD40L) permitted indefinite pancreatic islet allograft survival in 37 of 40 recipients that differed from islet donors at major and minor histocompatibility loci. The effect of the allogeneic small lymphocytes was donor antigen-specific. Neither treatment alone was as effective as combined treatment, although anti-CD40L by itself allowed indefinite islet allograft survival in 40% of recipients. Our interpretation is that small lymphocytes expressing donor antigens in the absence of appropriate costimulatory signals are tolerogenic for alloreactive host cells. Anti-CD40L antibody may prevent host T …

Expression Of Genes Encoding Antioxidant Enzymes In Preimplantation Mouse And Cow Embryos And Primary Bovine Oviduct Cultures Employed For Embryo Coculture., M B Harvey, M Y Arcellana-Panlilio, X Zhang, G A Schultz, A J Watson

Obstetrics & Gynaecology Publications

Preimplantation embryos from a variety of mammalian species contrast markedly in their response to culture in vitro. Murine preimplantation embryos display a wider tolerance than other mammalian species to culture environments, and this has contributed to the development of several effective defined culture media. Embryo coculture on somatic cells remains the most effective method of supporting reasonable rates of bovine preimplantation development in vitro. The patterns of gene expression for several antioxidant enzymes during preimplantation murine and bovine development were examined by use of the reverse transcription-polymerase chain reaction technique to determine whether the differential developmental capacity of mammalian preimplantation …

Asbestos Induces Nuclear Factor Kappa B (Nf-Kappa B) Dna-Binding Activity And Nf-Kappa B-Dependent Gene Expression In Tracheal Epithelial Cells., Yvonne M. Janssen, Aaron Barchowsky, Melinda Treadwell, Kevin E. Driscoll, B T. Mossman

Dartmouth Scholarship

Nuclear factor kappa B (NF-kappa B) is a transcription factor regulating expression of genes intrinsic to inflammation and cell proliferation--features of asbestos-associated diseases. In studies here, crocidolite asbestos caused protracted and dose-responsive increases in proteins binding to nuclear NF-kappa B-binding DNA elements in hamster tracheal epithelial (HTE) cells. This binding was modulated by cellular glutathione levels. Antibodies recognizing p65 and p50 protein members of the NF-kappa B family revealed these proteins in two of the DNA complexes. Transient transfection assays with a construct containing six NF-kappa B-binding DNA consensus sites linked to a luciferase reporter gene indicated that asbestos induced …

Astrocytes Derived From P53-Deficient Mice Provide A Multistep In Vitro Model For Development Of Malignant Gliomas., Alan M. Yahanda Md, Facs, J M Bruner, L A Donehower, R S Morrison

PCI Publications and Projects

Loss or mutation of p53 is thought to be an early event in the malignant transformation of many human astrocytic tumors. To better understand the role of p53 in their growth and transformation, we developed a model employing cultured neonatal astrocytes derived from mice deficient in one (p53 +/-) or both (p53 -/-) p53 alleles, comparing them with wild-type (p53 +/+) cells. Studies of in vitro and in vivo growth and transformation were performed, and flow cytometry and karyotyping were used to correlate changes in growth with genomic instability. Early-passage (EP) p53 -/- astrocytes achieved higher saturation densities and had …

The Rat Tram Flap: A Human Analogue?, Geoffrey G. Hallock Md

Department of Surgery

No abstract provided.

Takings Of Wildlife Under The Endangered Species Act After Babbitt V. Sweet Home Chapter Of Communities For A Great Oregon, Lawrence R. Liebesman, Steven A.G. Davison

All Faculty Scholarship

No abstract provided.

Just Go On, Mitch Raney

A Midwestern Review: A Magazine of the Arts and Issues at DePauw University

No abstract provided.

Who's More Cruel, Johnny Or Jenny? Sex Differences In Adults' Perceptions Of Cruelty To Animals By Children, Teresa Michelle Thompson

Undergraduate Honors Capstone Projects

As a symptom of Conduct Disorder, cruelty to animals (DSM IV, 1994) is often assessed via parental checklists (e.g., Child Behavior Checklist). However, little information exists on the criteria that adults use to make judgments of cruelty.

Increased Alpha 1(I) Procollagen Gene Expression In Tight Skin (Tsk) Mice Myocardial Fibroblasts Is Due To A Reduced Interaction Of A Negative Regulatory Sequence With Ap-1 Transcription Factor., Neena Philips, Reza I. Bashey, Sergio A. Jimenez

Department of Medicine Faculty Papers

The TSK mouse, a model of fibrosis, displays exaggerated connective tissue accumulation in skin and visceral organs including the heart. To study the mechanisms of myocardial fibrosis in TSK mice, we established several strains of TSK mice myocardial fibroblasts in culture and examined the regulation of collagen gene expression in these cells. These strains displayed increased collagen gene expression in comparison with myocardial fibroblasts established from normal mice. On an average, the TSK myocardial fibroblast cultures showed a 4-fold increase in collagen synthesis and 4.4- and 3.6-fold increases, respectively, in alpha 1(I) and alpha 1(III) collagen mRNA steady state levels. …

Alteration Of Wildlife Habitat As A Prohibited Taking Under The Endangered Species Act, Steven A.G. Davison

All Faculty Scholarship

No abstract provided.

Sensitization To Self (Virus) Antigen By In Situ Expression Of Murine Interferon-Gamma., Myung-Shik Lee, Matthias Von Herrath, Hans Reiser, Michael B.A. Oldstone, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Autoimmune disease results from inflammatory destruction of tissues by aberrant self-reactive lymphocytes. We studied the autoimmune potential of T lymphocytes immunologically ignorant of viral antigens acting as self antigens and whether the host defense molecule IFN-gamma could stimulate these cells to cytotoxic competency. For this purpose, we produced double transgenic mice expressing pancreatic IFN-gamma as well as lymphocytic choriomeningitis virus (LCMV) nucleoprotein (NP) or glycoprotein (GP) antigen. 100% of the NP+/IFN-gamma+ mice became diabetic before 2 mo of age, while none of the NP single transgenic littermates and only 10% of IFN-gamma single transgenic littermates did. Strikingly, NP+/IFN-gamma+ mice spontaneously …

Myasthenia Gravis-Like Syndrome Induced By Expression Of Interferon Gamma In The Neuromuscular Junction., Danling Gu, Lise Wogensen, Nigel A. Calcutt, Chunyao Xia, Simin Zhu, John P. Merlie, Howard S. Fox, Jon Lindstrom, Henry C. Powell, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Abnormal humoral responses toward motor end plate constituents in muscle induce myasthenia gravis (MG). To study the etiology of this disease, and whether it could be induced by host defense molecules, we examined the consequences of interferon (IFN) gamma production within the neuromuscular junction of transgenic mice. The transgenic mice exhibited gradually increasing muscular weakness, flaccid paralysis, and functional disruption of the neuromuscular junction that was reversed after administration of an inhibitor of acetylcholinesterase, features which are strikingly similar to human MG. Furthermore, histological examination revealed infiltration of mononuclear cells and autoantibody deposition at motor end plates. Immunoprecipitation analysis indicated …

The Animal Research Controversy: Protest, Process & Public Policy, Andrew N. Rowan, Franklin M. Loew, Joan C. Weer

Experimentation Collection

The controversy today regarding the use of animals in research appears on the surface to be a strongly polarized struggle between the scientific community and the animal protection movement. However, there is a wide range of opinions and philosophies on both sides. Mistrust between the factions has blossomed while communication has withered. Through the 1960s, 1970s and early 1980s, the animal movement grew in numbers and financial resources, and developed much greater public recognition and political clout. The research community paid relatively little attention to the animal movement for much of this period but, alarmed by several public relations coups …

G95-1244 Ventilation Fans: Efficiency And Maintenance, David P. Shelton, Gerald R. Bodman

University of Nebraska-Lincoln Extension: Historical Materials

This publication describes efficiency and design factors to consider when selecting a ventilation fan. Basic maintenance procedures needed for efficient operation are also discussed.

An energy efficient ventilation system requires good design and good equipment. Selecting efficient ventilation fans and keeping them in good working condition will keep operating costs down.

G95-1243 Ventilation Fans: Types And Sizes, Gerald R. Bodman, David P. Shelton

University of Nebraska-Lincoln Extension: Historical Materials

This publication describes the most common types of ventilation fans and compares their physical and performance characteristics.

Two general types of ventilation fans are commonly used for agricultural applications. Each type has many different blade diameters, materials, blade shapes, speeds, and motor horsepowers available. The desired airflow rate and the resistance to airflow through the ventilation system, or static pressure capabilities, are the two primary considerations when selecting a ventilation fan. Other considerations include maintenance requirements, noise levels, and energy efficiency.

G95-1253 Basic Principles Of Mastitis Control, Jeffrey F. Keown, Paul J. Kononoff

University of Nebraska-Lincoln Extension: Historical Materials

Mastitis in dairy cows is a frustrating, costly, and complex infection. A good, workable, effective preventive control program is presented in this NebGuide.

General Characteristics of Mastitis

Mastitis is an inflammation of the mammary gland usually caused by bacterial infection of udder tissues. Except for rare injuries, infectious bacteria or other pathogens, e.g. yeast, fungi, etc., enter the udder through the teat end. Mastitis in both clinical (obvious abnormality, visible to naked eye) and subclinical (unseen signs of abnormality, invisible to nake eye) stages is a frustrating, costly and complex disease that reduces the quality and quantity of milk.

Afap-120 A Variant Form Of The Src Sh2/Sh3-Binding Partner Afap-110 Is Detected In Brain And Contains A Novel Internal Sequence Which Binds To A 67-Kda Protein, D C Flynn, T C Koay, C G Humphries, A C Guappone

Faculty & Staff Scholarship

No abstract provided.

The Gtp-Bound Form Of The Yeast Ran/Tc4 Homologue Blocks Nuclear Protein Import And Appearance Of Poly(A)+ Rna In The Cytoplasm., Gabriel Schlenstedt, Claudio Saavedra, Jonathan D. Loeb, Charles N. Cole, Pamela A. Silver

Dartmouth Scholarship

Ran/TC4, a Ras-like GTP-binding protein, and its nucleotide exchanger, RCC1, have been implicated in control of protein movement into the nucleus and cytoplasmic accumulation of mRNA. Saccharomyces cerevisiae contains two homologues of the mammalian Ran/TC4, encoded by the GSP1 and GSP2 genes. We have constructed yeast strains that overproduce either wild-type Gsp1 or a form of Gsp1 with glycine-21 converted to valine (Gsp1-G21V), which we show stabilizes the GTP-bound form. Cells producing Gsp1-G21V have defects in localization of nuclear proteins; nuclear proteins accumulate in the cytoplasm following galactose induction of Gsp1-G21V. Similarly, cells producing Gsp1-G21V retain poly(A)+ RNA in their …

Standing Up To Mr. O. (1998) By Claudia Mills, Gareth B. Matthews

Middle Grades Books

Fifth-grade Maggie's refusal to dissect a worm in science class is the first step on a philosophically-complicated path of moral awakening that will lead to conflict with her family, her friends, and her school. Claudia Mills’ middle-grades novel Standing Up to Mr. O. is an ideal text to introduce a discussion of moral issues concerning the treatment of animals. Almost all the points one would want to include in such a discussion are raised here, and they appear in a way that makes them immediately accessible both to teenagers and to their parents and teachers.

The Interdependent Morality Directive: An Extension Of The Animal Rights And Environmental Ethics Debate, Gerald Anthony Voltura

UNLV Retrospective Theses & Dissertations

The Interdependent Morality Directive: An Extension of the Animal Rights and Environmental Ethics Debate offers a foundation that seeks to reconcile the contending arguments from the animal rights and the environmental ethics schools of thought. By constantly emphasizing the interdependency of all members of the biosphere, the Interdependent Morality Directive offers a unique perspective concerning how humans can learn to interact with the Earth's varied and diverse life forms. First, this thesis presents an analysis of two opposing camps: (1) the environmentalist argument as represented by Aldo Leopold's A Sand County Almanac (the land ethic) and Paul Taylor's Respect For …

The Chicken's Travels, Roman Karas, Fleet Library, Special Collections, Jan Baker

Animals

This book was created for Jan Baker's artists' book class.