Digital Commons Network^™

Microbiota Dynamics And Bacterial Pathogen Survival During Milk Kefir Fermentation And Storage, Léon Maughan, Leonard Koolman, Guerrino Macori, Christina Killian, Seamus Fanning, Paul Whyte, Declan Bolton

Research Outputs: 2025-Present

This study identified and investigated the relative abundance of bacterial and fungal (predominantly yeast) species in the milk kefir grains, during a typical fermentation (25◦C for 48 h) and subsequent chilled storage (4◦Cfor up to 30d) using Nanopore sequencing (16S and ITS amplicon). Challenge studies were also undertaken toexamine the behaviour of Listeria monocytogenes, Salmonella Senftenberg, Escherichia coli O157:H7, Staphylococcusaureus as well as non-toxigenic mesophilic and psychrophilic Clostridium spp. (Clostridium sporogenes and Clos-tridium gasigenes, respectively). Lactobacillus and Lactococcus were the predominant bacterial genera with relativeabundance of 91% and 2% in milk kefir grains, 32% and 63.7% after 24 h of …

Fire–Climate–Vegetation Linkages In A Quaking Aspen Forest During The Late Holocene, R. Justin Derose, Jesse L. Morris

Wildland Resources Faculty Publications

Fire plays an important role in shaping forested ecosystems around the globe. Unlike many other fire-driven forest types, our understanding of pre-settlement fire behavior in quaking aspen (Populus tremuloides) systems is limited. To better understand the frequency and severity of fires in a putatively stable quaking aspen forest, a small, key watershed was selected for sediment coring to reconstruct fire history, vegetation change, and climatic variability. The study aim was to explore the fire–climate–vegetation linkages in an aspen-dominated catchment. For the past ~4000 years this basin has been dominated by quaking aspen but also subalpine fir (Abies …

Handheld Hyperspectral Imaging Dataset Of Annual Sowthistle And Little Mallow Under Abiotic Stress For Machine Learning, Elijah Brunnengraeber, Tyler Cassidy, Dylan Woodbridge, Arun D. Jani, Arun K. Sharma

Biology, Agriculture and Chemistry Faculty Publications and Presentations

Machine learning has become an increasingly important tool for overcoming agricultural challenges by enabling efficient and consistent classification of crop-related data. Training such supervised models requires high quality labeled datasets. This work presents a dataset consisting of raw and preprocessed hyperspectral imaging (HSI) files capturing reflectance in the visible to near-infrared range (400–1000 nm) from two problematic weed species on California’s Central Coast: annual sowthistle (Sonchus oleraceus) and little mallow (Malva parviflora). Hyperspectral imaging provides rich spectral-spatial data cubes that can support the development of deep learning models and autonomous technology for precision weed management. Plants …

Thermosiphon Lab Experiment, Ella C. Perry, Zach Barry, Madison K. Felton, Lane A. Hunter

Mechanical Engineering

The Cal Poly Mechanical Engineering Department aims to develop a new lab centered on a thermosiphon solar system. This lab will introduce the operating principles of thermosiphons while integrating concepts from heat transfer, fluid dynamics, and thermodynamics. A thermosiphon functions by heating water using thermal energy. The lab will examine the efficiency of this energy transfer. The final lab will be tailored to enhance student learning while ensuring ease of use for the Mechanical Engineering Department.

Finishing Location Influences The Color Stability Of Steaks From Grass-Finished Bison Bulls, Garrett Weldy, Christina Bakker, Judson Grubbs, Keith Underwood, Carter Kruse, Ana Paula Salim, Surendranath Suman, Amanda Blair

Animal Science Research Report 2026

No abstract provided.

Observing Ph Of Grass-Fed Bison Heifer Gastrointestinal Tracts Postmortem, Ekaterina Lopez-Bondarchuk, Madison Higbee, Kallen Kafka, Ava Graham, Phillip Urso

Animal Science Research Report 2026

No abstract provided.

Hydropower Collegiate Competition 2026, Andres R. Arreola, Sammy D. Taporco, Kathryn E. Tinney, Ahmer S. Dhillon

Mechanical Engineering

The Hydropower Collegiate Competition (HCC) is sponsored by the Department of Energy’s (DOE) Water Power Technologies Office (WPTO) and is meant to offer university students an opportunity to solve complex hydropower challenges, build industry connections, explore career connections, and obtain a greater knowledge of hydropower’s potential in the United States. For this year’s competition, our team investigated a potential in-conduit hydropower site in California. Our work included creating a design package for a crossflow turbine related to our selected site and completing the community connections challenge. We also completed a siting and design report, community connections report, design and siting …

University Of Nebraska At Omaha Division Of Biomechanics And Research Development Annual Report 2023-2025, Uno Biomechanics

Biomechanics Annual Report

This report contains:

A letter from the Director: Dr. Nick Stergiou

Highlights articles

Center for Cardiovascular Research in Biomechanics (CRiB) articles

The Center for Research in Human Movement Variability (MOVCENTR) articles

People of UNO Biomechanics

Student Centered articles

Education Initiative articles

Resource Assessment Report No. 8: Pilbara Prawn Resource - 2024 Assessment, Inigo Koefoed, Rachel Marks, Scott Evans, Sharon Wilkin, Jason How

Resource Assessment Reports

The Pilbara Prawn Resource (Resource) is accessed by three managed commercial fisheries; the Exmouth Gulf Prawn Managed Fishery (EGPMF), the Onslow Prawn Managed Fishery (OPMF) and the Nickol Bay Prawn Managed Fishery (NBPMF). All three fisheries are managed under effort controls, with the EGPMF and OPMF targeting brown tiger prawns (BTP; Penaeus esculentus), western king prawns (WKP; Penaeus latisulcatus), blue endeavour prawns (BEP; Metapenaeus endeavouri), and lesser quantities of banana prawns (Penaeus merguiensis). The NBPMF primarily targets banana prawns while also catching BTP, WKP, and BEP in lower numbers. These fisheries are situated adjacent to one another along the Pilbara …

A Phenomenological Study Of The Lived Experiences Of Vicarious Trauma And Emotional And Somatic Resource Replenishment Among K-12 School Principals In Suburban Communities, Rachael A. Garcia

Education Doctoral

This qualitative study, grounded in the job demands-resources (JD-R) theory, explores the lived experiences of K-12 principals in suburban communities as they encounter vicarious trauma (VT). The study aims to fill a gap in the research by examining how these leaders deliberately employ strategies to replenish their emotional and somatic resources and develop resilience. The role of school principals has evolved significantly, now requiring them to provide substantial social-emotional support for both students and staff, which exposes them to others' traumatic experiences. This exposure can lead to vicarious trauma, a deep psychological transformation resulting in emotional exhaustion, compassion fatigue, and …

Human Stem Cell-Based Embryo Models: Innovation, Ethics, And Policy, Alfonso Martinez Arias, Thorold Theunissen, Et Al.

2020-Current year OA Pubs

The aim of this White Paper is to establish a foundational framework for research, technological development, and regulation in the emerging field of stem cell-based embryo models (SCBEMs). These models, generated from Pluripotent Stem Cells, are designed to recapitulate essential events in early stages of human development. They have the potential to illuminate the early stages of embryo development and implantation and hold promise as an avenue to address global health challenges, including infertility and pregnancy loss, congenital, neonatal and adult conditions, and the need for organ transplants. While SCBEMs are not a substitute for human embryos, their tractability for …

Synergistic Effects Of Diabetic Neuropathy And Kidney Function Decline On Second Metatarsal Skeletal Health And Foot Function: A Pilot Study, Victor A Cheuy, Saghi Sadoughi, Hasung Moon, Jalen Ellis, Rodney Gross, David R Sinacore, Mary K Hastings, Richard B Souza, Galateia J Kazakia

2020-Current year OA Pubs

A discouraging increase in diabetes-related nontraumatic lower-extremity amputations (NLEA) has occurred over the last decade. Individuals with diabetic neuropathy (DMPN) and CKD are at the highest risk for NLEA. Despite the critical implications, little is known about the factors that initiate the cascade of events leading to NLEA, limiting early and effective interventions. We aimed to develop and implement a technique for quantifying metatarsal bone density and microstructure using HR-pQCT. Twelve participants were enrolled:

Spatial Transcriptomic Profiling Of Decalcified Murine Musculoskeletal Samples Via Xenium Prime 5k, Lindsey Lammlin, Huong X Tran, Aanya Mohan, Michael D Newton, Scarlet C Howser, Stephen J Redding, Christina A Capobianco, Kurt D Hankenson, Alexander J Knights, Tristan Maerz

2020-Current year OA Pubs

Successful generation of high-quality spatial transcriptomics data from murine musculoskeletal tissues has been impeded by the challenge of preserving RNA integrity through the harsh tissue processing steps required for histological sectioning. In particular, the need to thoroughly fix and decalcify mineralized tissues has proven problematic. We detail a comprehensive sample processing pipeline for 3 common murine musculoskeletal tissue samples, enabling high-quality transcript detection via imaging-based spatial transcriptomics using the Xenium Prime 5K platform from 10x Genomics. Our protocol outlines methodological details for transcardiac perfusion, fixation, decalcification, paraffin processing, and a sample co-embedding strategy facilitating anatomically consistent and simultaneous sectioning of …

Expanding The Omop Common Data Model To Support Extracorporeal Life Support Research, Clemens Rieder, Ahmed Said, Et Al.

2020-Current year OA Pubs

OBJECTIVE: Despite the increased availability of electronic health records, open-source standardized data collection to facilitate high-resolution data during extracorporeal life support (ECLS) is lacking. This project aimed to assess the Observational Medical Outcomes Partnership Common Data Model (OMOP CDM) for interoperability to store data sufficiently generated in the context of ECLS and to develop a custom data model expansion in case the OMOP CDM proved insufficient.

DESIGN AND SETTING: The OMOP CDM was analyzed qualitatively by expert consensus for its capability to capture data relative to ECLS as well as the presence of fitting ECLS-related concepts. Database entries necessary to …

Role Of Households With Children In Community Spread Of Multidrug-Resistant Enterobacterales, St. Louis, Missouri, Usa, Barrett Breeze, Ahmed Babiker, Sreenivas Konda, Alaina L Robinson, Stefan J Green, Catherine C Babbs, Federico Cunha, Katherine Y Shen, India Shepherd Hammond, Stephanie A Fritz, Latania K Logan

2020-Current year OA Pubs

Community-acquired multidrug-resistant (MDR) Enterobacterales bacteria are an increasing public health concern, yet whether households play a role in community spread remains unclear. We investigated 150 households with children in St. Louis, Missouri, USA, for MDR Enterobacterales. We cultured swab specimens from household members and environmental surfaces for identification and antimicrobial susceptibility testing. We also performed whole-genome sequencing in the 53 (35%) households where >1 MDR Enterobacterales species were recovered. Enterobacter hormaechei predominated, followed by Klebsiella pneumoniae and Pantoea species. Whole-genome sequencing revealed closely related strains shared between persons and environmental surfaces, suggesting potential intra-household transmission. We identified >1 horizontal gene …

U.S. Regulation And Litigation Regarding Gmos: A Cautionary Tale Of A Piecemeal Framework, Michael R. Reese, George V. Granade

Northern Illinois University Law Review

Bioengineered crops using genetically modified organisms (GMOs or GM) are big business in the United States. In little more than two decades, GM agriculture constitutes more than 90% of crops such as corn, soy, and canola. The acceptance of GMO foods by consumers, however, has not been as robust—leading to demand for labeling regulations so consumers can identify whether their foods contain GMOs. U.S. law governing GMOs, particularly food labeling, lacks a comprehensive regulatory scheme and is instead highly fragmented. The Food and Drug Administration (FDA) has debated how to label such foods for over a decade, issuing no rules …

Bringing The Museum To The Library: A Collaboration Between A Natural History Museum And An Academic Science Library, Angela D. Price, Jean Bossart

Collaborative Librarianship

A persistent challenge on college campuses is effectively connecting students with the wide array of university resources available to them. At the University of Florida, an opportunity to address this challenge emerged when the Florida Museum of Natural History (FLMNH), a major campus institution with extensive scientific collections, was scheduled for a 15-month closure due to renovations. In response, librarians at the Marston Science Library initiated a collaborative partnership with FLMNH, offering the library as a venue for science-related events and programming. This collaboration enhanced student engagement, added a non-traditional venue for science learning and interaction, and proved mutually beneficial …

Roots And Greens: Discovering The Power Of Indigenous Produce, Abiodun T. Atoloye, Sherif O. Olasoji, Ebunoluwa G. Ibidapo

Nutrition, Dietetics, and Food Sciences Courseware

The curriculum was designed in alignment with the Cognitive Theory of Multimedia Learning (CTML), Empowerment Theory (ET), and Social Cognitive Theory (SCT) frameworks. These frameworks were used to determine the selection and sequencing of learning activities. As reflected in CTML principles, the use of texts, images, infographics, and videos enhances knowledge retention and information comprehension and helps prevent cognitive overload. Through activities, homework, and family engagement, the principles of ET were incorporated into the curriculum to facilitate participants’ perceived control over food-related decisions. Additionally, by applying the principles of SCT, the curriculum incorporates scenario-based questions, opportunities to engage with peers, …

Comparable Endogenous Immune Reconstitution, Car-T Expansion, And Cytokine Profiles With Bendamustine Versus Fludarabine/Cyclophosphamide Lymphodepletion, Matthew Schwede, Sarah Hawley, Zachary Reitz, Faith Kierney, Neil Bailey, Tenzin Tsomo, Laiken Lopez, Terry Gilbertson, Christopher A Carney, Doug Kieper, Kelly Paulson, Krish Patel

All Poster Presentations

No abstract provided.

From Lineage Discovery To Conservation Prioritisation, Zachary G. Macdonald, Joscha Beninde, Julian R. Dupuis, Thomas W. Gillespie, H. Bradley Shaffer, Gregory F. Grether

Entomology Faculty Publications

Accurate inferences of diversification and evolutionary processes depend on knowing how many independently evolving lineages exist within nominally widespread taxa. Uncertainty in lineage number and composition also limits our ability to meaningfully prioritise conservation efforts. Addressing these knowledge gaps requires frameworks that sequentially identify distinct lineages, assess mechanisms that contribute to their divergence, and quantify spatial and environmental determinants of their genomic diversity. With these goals in mind, we apply whole-genome, ecological, and morphological analyses to the American rubyspot damselfly (Hetaerina americana), a widespread and well-studied North American insect that has been long regarded as a single species of least …

Owning Geothermal Energy, Gabriel Eckstein

Faculty Scholarship

Geothermal energy holds vast potential for decarbonizing the U.S. energy system, yet the sector remains significantly underdeveloped, supplying only 0.4% of the nation's electricity. A principal barrier to growth is the fragmented and ambiguous legal landscape currently governing ownership of geothermal resources: thirty-three states lack any clear statutory or common-law framework identifying who owns subsurface thermal energy. This Article examines the physical characteristics of geothermal energy, recent developments in the U.S. geothermal sector, and the principal doctrinal approaches that might govern ownership of subsurface heat, including the ad coelum doctrine, common-pool and open-access frameworks, the rule of capture, and public …

Shape-Based Profiling Of Cortical Bone Mineral Density In Growing Children: A Proof-Of-Concept Pqct Study, Anna Maria Markarian, R. Taaffe, Daniel Galvão, Jodie Cochrane Wilkie, Carolyn J. Peddle-Mcintyre, Daniel J. Schiferl, Robert U. Newton

Research outputs 2022 to 2026

Background: Understanding how bones adapt during growth is essential for promoting lifelong skeletal health. Peripheral quantitative computed tomography (pQCT) is a widely used imaging technique for assessing volumetric bone mineral density (vBMD) in pediatric populations; however, conventional methods may obscure subtle cortical adaptations. The primary aim of this study was to evaluate the feasibility of shape-based cortical vBMD profiling, with an exploratory objective of assessing its sensitivity to inter-individual variation in lean mass index (LMI). Methods: Eighteen children (6–12 years) underwent pQCT scanning at the 38% and 66% tibial sites (distal and proximal diaphysis). Cortical vBMD was quantified across three …

The Interplay Between Osteosarcopenia And Intrinsic Capacity: Insights And Associations With All-Cause Mortality In The Toledo Study For Healthy Aging, Ivan Baltasar-Fernandez, Pedro L. Valenzuela, Irene Rodríguez-Gómez, Fabio Quiñonez-Bareiro, Luis M. Alegre, Konstantinos Prokopidis, Leocadio Rodriguez-Mañas, Ignacio Ara, Oscar Rosas-Carrasco, Lana J. Williams, Robinson Ramirez-Vélez, Julie A. Pasco, Guy Hajj-Boutros, Raman Agnihotram, Marc Sim, Wayne Lok Ok Choo, Mahdi Imani, Ana M. Ayala-Copete, Julia Chabot, Miguel G. Borda, Nailton J. Neto, Claire Godard-Sebillotte, Howard Bergman, Anna Andrianova, Hidenori Arai, Pierrette Gaudreau

Research outputs 2022 to 2026

Background: Osteosarcopenia—the coexistence of osteopenia/osteoporosis and sarcopenia—is associated with numerous adverse health outcomes, but its association with intrinsic capacity (IC) remains unknown. We aimed to explore the associations of osteosarcopenia with IC and all-cause mortality in older adults. Methods: We conducted a cross-sectional analysis of IC and a prospective analysis of all-cause mortality in 1142 participants from the Toledo Study for Healthy Aging (75.1 ± 6.0 years, 53.1% women). Participants were classified as having no musculoskeletal disorders, osteopenia/osteoporosis, sarcopenia, or osteosarcopenia (DXA- and EWGSOP2-based criteria). IC was operationalized as a composite score integrating locomotion, cognition, psychological, vitality, and sensory domains. …

Development And Validation Of The Lifetime Sense Of Safety Scale In Kenyan Adults, Karen Blackmon, Anne Nyambura, Anne Gitere, Levi Muyela, Catherine Onyancha, Kendi Muchungi, Catherine Ajalo, Litha Musili, Rachel Maina, Edna Bosire, Lukoye Atwoli, Zul Merali, Chinedu Momoh

Brain and Mind Institute

Background. Safety supports psychological wellbeing and potentially shapes brain aging yet existing scales overlook cumulative lifespan safety experiences.

Methods. To address this gap, we developed and validated the Lifetime Sense of Safety (LSS) scale in Kenyan adults. The LSS uses visual analog scaling with a red-to-green color gradient to retrospectively rate felt safety across life stages and settings. We evaluated scale psychometrics and associations with social determinants of health and indicators of brain health.

Results. The LSS shows adequate internal consistency and test-retest reliability, with two distinct but correlated factors representing childhood and adulthood safety. Women and …

Contextual Variability In Under-Diagnosed Cardiometabolic Disease And Cognitive Vulnerability Among Populations At High Risk For Alzheimer's Disease And Related Dementias, Nwanyieze Jiakponnah, Joseph Curran, Tamlyn Watermeyer, Jasmit Shah, Litha Musili, Stanely Onyango, Benard Aliwa, Zul Merali, Karen Blackmon, Chinedu Momoh

Brain and Mind Institute

Underdiagnosis of cardiometabolic risk factors (CMRFs) may represent an unrecognised biological pathway contributing to dementia risk; yet remains poorly characterised in African and African diaspora populations. We quantified the prevalence and determinants of underdiagnosed hypertension and abnormal glycaemia across four cohorts comprising up to 7000 adults aged ≥40 years from Nigeria, Kenya, and The United States: Indianapolis, and North Texas. Underdiagnosis was defined as absence of self-reported diagnosis despite elevated systolic blood pressure ( ≥130 mmHg) or fasting blood glucose ( ≥100 mg/dL). Cohort-stratified analyses examined demographic, socioeconomic, cognitive, Alzheimer’s genetic, and blood-based biomarker correlates. Underdiagnosis was pervasive in African …

Navigating Precarity: Resourcefulness And Interdependence In The Upper Valley Dairy Industry, Benna Mcdermott

Geography Undergraduate Senior Theses

No abstract provided.

Informal Humans? Rights To A Clean Environment In Bangkok’S Informal Settlements, Maisie Pike

Geography Undergraduate Senior Theses

No abstract provided.

Adhesion Index Integrates Cell And Bacterial Responses To Titanium Across Protein Conditioning And Surface Roughness, Sahar Afshari, James D. Boyd, Victoria L. Stanton, Arnold Stromberg, Katherine L. Thompson, David M. Devito, Craig S. Miller, Martha E. Grady

Mechanical Engineering Faculty Publications

Biofilm formation is a primary cause of implant-associated infections in dental and orthopedic devices. Early interactions between bacteria and host cells at the implant surface critically influence subsequent infection or successful integration. Therefore, biocompatibility screening should evaluate both biofilm formation and cell adhesion. Adhesion Index, defined as the ratio of cell adhesion to biofilm adhesion, provides a non-dimensional number to optimize towards that end. In this work, the laser spallation technique is implemented to compare the effects of implant surface characteristics (rough and smooth titanium) and protein coatings (blood plasma and fibronectin) on bacterial biofilm (Streptococcus mutans, Staphylococcus aureus) and …

Striveforpride: Online Program To Promote Sexual And Gender Minority Well-Being, Shayna R. Greenberg

Loma Linda University Electronic Theses, Dissertations & Projects

Sexual and gender minority (SGM) young adults are more likely to experience anxiety, depression, and stress relative to non-SGM peers. Digitally-administered mental health interventions show potential for reducing mental health distress in the general population. However, limited reporting of SGM demographics and adherence to online interventions constrain uptake and effectiveness. The present study explored the effect of an existing evidence-informed, self-guided, online-based mental health intervention, StriveWeekly, on internalizing symptoms among SGM university students. The sample consisted of 160 SGM university students, identifying as female, women, or feminine (63.1%), bisexual (36.10%), and Non-Hispanic (77.5%). Cluster randomization was employed on the basis …

Comparable Endogenous Immune Reconstitution, Car-T Expansion, And Cytokine Profiles With Bendamustine Versus Fludarabine/Cyclophosphamide Lymphodepletion, Matthew Schwede

All Podium Presentations

No abstract provided.