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Full-Text Articles in Entire DC Network
Flood Adaptation And Productivity Of Savanna Grasses, E Gonzalez Jimenez, A Escobar
Flood Adaptation And Productivity Of Savanna Grasses, E Gonzalez Jimenez, A Escobar
IGC Proceedings (1977-2023)
Savannas subjected to seasonal flooding occupy· vast areas of the Tropics. Above-ground biomass values for Venezuelan flooded savannas are high and related to the longer availability of water. C3 plants colonized the waterlogged unit and showed the highest productivity and nutritive value. This natural system permits a prolonged grazing season. These first results show the great potential for animal production of this ecosystem, stressing the need for coordinated research efforts in order to gain a better understanding of it.
Studies On Site, Vegetation And Intensification Of Temporarily Flooded Grassland Of The Lower Oder River Near Schwedt, Gisbert Schalitz
Studies On Site, Vegetation And Intensification Of Temporarily Flooded Grassland Of The Lower Oder River Near Schwedt, Gisbert Schalitz
IGC Proceedings (1977-2023)
On the temporarily flooded grassland of the lower Oder River near Schwedt, yields can be considerably increased by establishing highyielding Phalaris arundinacea stands. In the drier zone it is indispensable to kill the old sward. To this effect, adequate methods should be chosen according to the type of existing vegetation. In the range of moisture-tolerant plant associations, increased nitrogen fertilization will allow to build up high-yielding pure stands in short time.
Lead Content In Meadow Vegetation From NonContaminated Areas, Andrzej Sapek
Lead Content In Meadow Vegetation From NonContaminated Areas, Andrzej Sapek
IGC Proceedings (1977-2023)
In 270 samples of selected meadow plant species, taken from natural sites and permanent meadows in the 1st-cut period, the lead content was determined. Mean lead content in particular species fluctuated from 0.36 ppm in Phalaris arundinacea to 1.38 ppm in Carex vulpina. On a whole, the lead content was lower in grasses than in herbs. No relationship between the total lead content in soil and plants has been proved. On the other hand, a positive and often significant relationship between the lead content in particular plant species and their potassium, copper, nickel, and phosphorus contents was observed.
Influence Of Plant Nutrition And Method Of Utilization On The Accumulation And Decomposition Of Root Matter In Meadow Soils, Yan Liyv, Rikhard Toomre
Influence Of Plant Nutrition And Method Of Utilization On The Accumulation And Decomposition Of Root Matter In Meadow Soils, Yan Liyv, Rikhard Toomre
IGC Proceedings (1977-2023)
Root matter formation of forage plants depends primarily on soil fertility. In fertile soils and under intensive fertilization less root matter is formed than in soils of low fertility. The decomposition of root matter as influenced by microbiological processes was found to be more intensive under grazing than under mowing. The application of high rates of nitrogen fertilizer (more than 200 kg N/ha) over longer periods is likely to cause undesirable changes in the relationship between forage plants and soil microorganisms, this resulting in yield decline and in lower efficiency of the fertilizers applied.
The Influence Of Grassland Plants Ort The Accu1nulation Of Humus And Nitrogen In The Soil In The Conditions Of The Estonian Ssr, Arnold Sau
IGC Proceedings (1977-2023)
The accumulation of nitrogen and humus in soil under perennial grassland plants was studied between 1965 and 1974 in a trial laid out on carbonated reddish brown sandy loam moraine of original levels of 0.22 % humus and 18 m %1 nitrogen, the C: N ratio being 7 .2. Nitrogen and humus accumulation in the moraine was found to proceed the faster, the higher were the yields of above-ground and underground plant matter. In the course of 10 years, the humus content in the upper 5 cm of soil reached 1.92 to 2.26 % for the higheryielding variants (grasses+ 150 …
Nitrogen Status Of A Shortgrass Prairie, Pasture Ecosystem, Robert G. Woodmansee, Jerrold L. Dodd, Rudolph A. Bowman, Charles E. Dickinson
Nitrogen Status Of A Shortgrass Prairie, Pasture Ecosystem, Robert G. Woodmansee, Jerrold L. Dodd, Rudolph A. Bowman, Charles E. Dickinson
IGC Proceedings (1977-2023)
Nitrogen was partitioned among soil organic matter, living and dead plant material, microorganisms, above-ground and below-ground animals, and mineral N in a shortgrass prairie, pasture ecosystem in eastern Colorado, USA Soil organic matter contained the largest amount of N (89.5 %), and dead plant material contained 6.8 % of the total N in the system. Living organisms contained 3.4 % of the N in the ecosystem. Mineral N accounted for 0.2 %. Less than 1.0 % of the N in the system was located above ground. The amount of N immobilized in animals was small.
Nutrient Leaching In Hydromorphic Grassland Soils As Established In Lysimeters, Gerhart Mundel
Nutrient Leaching In Hydromorphic Grassland Soils As Established In Lysimeters, Gerhart Mundel
IGC Proceedings (1977-2023)
Lysimeter experiments were laid out for studying the leaching of nitrogen, calcium, potassium, and phosphorus from different hydromorphic soils. Most of the hydromorphic soils, above all those of low-bog sites, have to be considered as instable open systems from the very beginning of their cultivation. Matter removal well exceeds the amount of matter introduced into these soils. The degree of nutrient leaching in most cases is clearly dependent on the soil humus status. Attention is drawn to the intensive calcium leaching and the low level of nitrogen removal as compared with anpydromorphic soils. The level of potassium leaching is closely …
Available Soil Nutrients Related To Species Composition Of Some Meadow Communities In North-Western Bohemia, Emilie Balatova-Tulackova
Available Soil Nutrients Related To Species Composition Of Some Meadow Communities In North-Western Bohemia, Emilie Balatova-Tulackova
IGC Proceedings (1977-2023)
The pH value as well as the content of humus and some available nutrients (Ca, Mg, K, P, N) were assessed in soil samples taken from the upper part of the rhizosphere in 15 different types of moist meadows situated in northwestern Bohemia. The results obtained were evaluated mainly from the point of view of the occurrence of Ca, K, and Pin soil related to the species composition (which is the decisive factor in its classification in the phytocenological system). The greatest indicative value is shown by plants with relatively narrow ecological amplitude in the given phytocenological bounds, such as …
Yearly Nitrogen Budget In Natural And Fertilized Grasslands Of A Bohemian-Moravian Highlands District, Milada Findeisova, Vladimir Ruzicka, Blanka Ulehlova
Yearly Nitrogen Budget In Natural And Fertilized Grasslands Of A Bohemian-Moravian Highlands District, Milada Findeisova, Vladimir Ruzicka, Blanka Ulehlova
IGC Proceedings (1977-2023)
Simplified models are presented of nitrogen cycling in the three grassland ecosystems, as compiled from soil, plant, rain- and lysimetric water analyses pertaining to an experimental site near Kamenicky, in the nature conservancy area Zdarske vrchy in the Bohemian-Moravian highlands. The plant stands, all originally classified as Polygalo-Nardetum strictae, include a control and two stands as modified by two levels of fertilization for three years. The increment of nitrogen harvested decreases considerably at the higher level of fertilization, while the nitrogen losses from the ecosystem increase and the atmospheric nitrogen fixation decreases dramatically.
Mineral Composition Of Vegetation Related To Primary Production In Alluvial Meadow Ecosystems, Jana Jakrlova
Mineral Composition Of Vegetation Related To Primary Production In Alluvial Meadow Ecosystems, Jana Jakrlova
IGC Proceedings (1977-2023)
Concentration and ratio of mineral nutrients, dynamics of mineral nutrient uptake per 1 m2, and the contents of mineral nutrients in permanent biomass reserve and in maximum biomass were assessed in three selected types of meadow ecosystems representing a natural hydrological series in a given area (dry, moist, wet). The assessment was based on chemical analyses (N, P, K, Na, Ca, Mg) and production analysis of above-ground and underground parts of defined vegetation.
Total above-ground biomass produced per year was estimated (from the dry type to the wet one) at 706, 1070, 2430 g • W • m-2, respectively; total …
Biomass Budget And Energy Flow In Alluvial Meadow Ecosystem, Milena Rychnovska, Blanka Ulehlova, Jana Jakrlova, Marta Tesrova
Biomass Budget And Energy Flow In Alluvial Meadow Ecosystem, Milena Rychnovska, Blanka Ulehlova, Jana Jakrlova, Marta Tesrova
IGC Proceedings (1977-2023)
Primary production, production processes, and decomposition have been studied in alluvial meadow ecosystems for 6 years. Water has been found to be the decisive factor in the energy flow and mineral elements turnover. The quality and quantity of production and decomposition processes depend on the occurrence and duration o( floods. Permanent pool of underground biomass in Alopecurus pratensis stand makes 45.3 %, permanent pool of shoot biomass makes 54.7 % of the total biomass. 19.4 % of the total biomass is taken off as forage. 11. 7 % of shoot biomass is decomposed during the summer season, and 3 .1 …
Current Aspects Of Eco-Physiological Studies Of Meadow Plants, Momcilo Konic
Current Aspects Of Eco-Physiological Studies Of Meadow Plants, Momcilo Konic
IGC Proceedings (1977-2023)
Basic parameters applied in eco-physiological studies of meadow plants are: water balance (hydration, transpiration intensity, amounts and forms of water, water deficit, etc.), assimilation balance (CO2 balance, assimilation performance, compensation effect), matter production. Eco-physiological studies should be followed by microclimatic measurements and the anatomical analysis of the meadow plants under study. Eco-physiological studies of meadow plants, if performed in a complex and systematic way, constitute the safest basis for detennining the characters of the entire meadow plant community under the concrete conditions of natural factors prevailing in terms of natural conditions and human influence.
Landscape Management Problems Of Grassland Utilization In Mountainous Regions Of The Gdr, Peter Hentschel
Landscape Management Problems Of Grassland Utilization In Mountainous Regions Of The Gdr, Peter Hentschel
IGC Proceedings (1977-2023)
In the medium-altitude mountains of the GDR, many meadows cannot be utilized for intensive forage production. Because of their importance for recreation, game feeding and nature conservation, general afforestation is not possible. A model is presented that allows for every meadow to choose between intensive utilization, afforestation, and reservation for other social needs. Management methods for meadows not suitable for intensive utilization, especially the employment of herbicides, are discussed.
Mitigating An Influenza Outbreak At A Residential Youth Summer Camp, Sandra D. Shapiro, Rachel Thomas, Stacey A. Hobbick, Bretta Weissman, Erica Gottlieb, Rebecca Spielman
Mitigating An Influenza Outbreak At A Residential Youth Summer Camp, Sandra D. Shapiro, Rachel Thomas, Stacey A. Hobbick, Bretta Weissman, Erica Gottlieb, Rebecca Spielman
CompassPoint
No abstract provided.
Fall 2024 - Volume 34, Number 2, Compass Point
Can Ungrading Make Sport Management Education More Practical And Engaging?, Michael A. Odio
Can Ungrading Make Sport Management Education More Practical And Engaging?, Michael A. Odio
The COSMA Journal
In this essay I take on the subject of grades in sport management education. While grades are used as a measure of success across higher education, they are an imperfect measure of achievement and learning and have major limitations in terms of student employability. The ungrading movement brings attention to the ways grades can harm learning by shifting attention away from students’ development, and instructor or peer feedback, and intrinsic motivation, while incentivizing compliance and risk-aversion. These harms directly affect what are arguably some of the most important aspects of sport management education, as when grades are the main incentive, …
Technical Disclosure: A Multi-Variable, Object-Oriented Database Schema And Api Protocol For Ai-Guided, Performance-Based Curriculum Routing, Christopher L. Eckes
Technical Disclosure: A Multi-Variable, Object-Oriented Database Schema And Api Protocol For Ai-Guided, Performance-Based Curriculum Routing, Christopher L. Eckes
Defensive Publications Series
This technical disclosure details the architecture for an automated, multi-variable, object-oriented data schema and Application Programming Interface (API) designed to execute performance-based curriculum routing without age-segregated grade boundaries. The system structures diverse academic, kinetic, and technical disciplines into standardized, interoperable data objects called Discipline Blueprints. Each blueprint enforces a non-linear advancement pipeline governed by two immutable checkpoints: a zero-assist algorithmic velocity sprint and a physical/systemic product generation milestone. A localized machine learning agent parses real-time telemetry from user interactions, dynamically matching individual cognitive profiles against localized workforce shortages and trade vectors to prevent network drop-out. Crucially, the architecture incorporates an …
From Ai To Alien(S): Universal Alignment Principles And The Architecture Of Inter-Species Communication Metrics, Christopher L. Eckes
From Ai To Alien(S): Universal Alignment Principles And The Architecture Of Inter-Species Communication Metrics, Christopher L. Eckes
Defensive Publications Series
The critical bottleneck in both contemporary Artificial Intelligence (AI) alignment and the Search for Extraterrestrial Intelligence (SETI) is anthropomorphic bias. Modern AI alignment models rely heavily on human linguistic structures, reinforcement learning from human feedback (RLHF), and qualitative ethical frameworks. These models degrade rapidly when scaled to non-human or multi-agent networks. Similarly, traditional SETI protocols look for messages encoded in cultural or linguistic formats that assume shared biological or social evolutionary tracks.
This paper introduces a foundational framework for AI-Alien Alignment Principles (AAAP). By treating communication as a problem of non-linear dynamical systems integration, this model establishes Topology, Vector Calculus, …
Molecular Detection Of Chlamydiales In Captive Zoo Animals At Songkhla Zoo, Southern Thailand, Rawikan Inchuai, Sunisa Khongthong, Boontarikaan Wititkornkul, Naruepan Wongpluksin
Molecular Detection Of Chlamydiales In Captive Zoo Animals At Songkhla Zoo, Southern Thailand, Rawikan Inchuai, Sunisa Khongthong, Boontarikaan Wititkornkul, Naruepan Wongpluksin
The Thai Journal of Veterinary Medicine
Several species of Chlamydia are recognized as significant zoonotic pathogens that infect a wide range of hosts, including humans. This study aimed to investigate the occurrence and genetic characteristics of Chlamydiales–related bacteria in captive animals at Songkhla Zoo, southern Thailand. A total of 92 cloacal and rectal swab samples were collected from mammals (n = 22), birds (n = 55), and reptiles (n = 15). Genomic DNA was screened using polymerase chain reaction (PCR) targeting the Chlamydiales 16S rRNA gene and the ompA gene specific for Chlamydia psittaci. Positive amplicons were subjected to Sanger sequencing and phylogenetic analysis. A …
Explainable Machine Learning Revealing The Impact Of Mental And Physical Health On Arthritis, Ma Shams, S Fatema, Dmh Islam, A Datta, David Eisenberg, Dt Mridula, J Mawa, A Sultana, N Ahmed, M Islam, Ts Pias
Explainable Machine Learning Revealing The Impact Of Mental And Physical Health On Arthritis, Ma Shams, S Fatema, Dmh Islam, A Datta, David Eisenberg, Dt Mridula, J Mawa, A Sultana, N Ahmed, M Islam, Ts Pias
Department of Information Management and Business Analytics Faculty Scholarship and Creative Works
Objectives To develop a robust and interpretable machine learning framework for arthritis risk prediction and to identify important risk factors associated with the disease.Methods This study used four datasets, which are Behavioral Risk Factor Surveillance System (BRFSS) 2019, BRFSS 2021, National Health Interview Survey (NHIS) 2020 and NHIS 2022. We evaluated 11 machine learning and deep learning architectures including a custom stacked ensemble combined with five resampling techniques to address class imbalance and 9 imputation methods to handle missing data. Area under the receiver operating characteristic curve (AUROC), sensitivity, specificity and balanced accuracy are used to evaluate model performance, with …
Identification Of Active Compounds And Antidiabetic Activity Of Red Shoot (Syzygium Myrtifolium) Leaves Extract With In Vitro And In Silico Methods, Muhamad Iqbal Prakoso, Siti Warnasih
Identification Of Active Compounds And Antidiabetic Activity Of Red Shoot (Syzygium Myrtifolium) Leaves Extract With In Vitro And In Silico Methods, Muhamad Iqbal Prakoso, Siti Warnasih
Pharmaceutical Sciences and Research
Diabetes mellitus (DM) is a chronic metabolic disease that affects approximately 537 million people worldwide (1 in 10 individuals), and its prevalence is projected to reach 783 million by 2045. One of the plants that has the potential to be an antidiabetic is red shoots (Syzygium myrtifolium) since it contains various secondary metabolite compounds such as flavonoids, triterpenoids, steroids, saponins, phenolics, and anthocyanins. This study aimed to evaluate the antidiabetic properties of red shoot leaves extract and to identify and predict interactions between its active compounds and the α-glucosidase receptor. Ethanol, ethyl acetate, and n-hexane extracts of red …
Repetition As Ritual Structure: Sound, Movement, And Electrodermal Activity In A Hmong Shaman Ceremony, Maile Williams
Repetition As Ritual Structure: Sound, Movement, And Electrodermal Activity In A Hmong Shaman Ceremony, Maile Williams
Theses and Dissertations
This thesis examines the relationship between repetitive sound, physiological arousal, and embodied participation in a Hmong shamanic ritual. Drawing on scholarship about music, trance, absorption, repetition, and Hmong ritual journeying and communication, I consider how sonic layers such as chant, rattle and gong help structure ritual time and sustain the shaman's altered state. The project uses electrodermal activity (EDA) data from wearable sensors, video and audio recordings, and my own event coding of sonic and physical activity during a selected 30-minute section of a recorded Hmong shaman ceremony. By aligning EDA data with a timeline of chanting, rattling, gong playing, …
Gust-Induced Aerodynamic Performance In Insects' Forward Flapping Flight, Arash Farsani, Ori Stearns, Gal Ribak, Roi Gurka
Gust-Induced Aerodynamic Performance In Insects' Forward Flapping Flight, Arash Farsani, Ori Stearns, Gal Ribak, Roi Gurka
Physics and Engineering Science
Flapping-wing flight is inherently unsteady, where atmospheric gusts can substantially degrade the aerodynamic performance when their characteristic time scales are comparable to the wingbeat period. This study presents a numerical investigation and time–frequency characterization of gust-induced aerodynamic response of flapping wings of varying size, inspired by the flight of a longhorn beetle, Batocera rufomaculata. Geometrically similar wings spanning the biological range were simulated in forward flight under identical prescribed kinematics and a transient frontal gust with a smoothly ramped profile. Three-dimensional unsteady Reynolds-averaged Navier–Stokes simulations using the shear stress transport k–ω turbulence model were performed to resolve the instantaneous pressure …
Upar-Guided Dendrimer Gel Nanoparticles Reprogram Inflammation To Stabilize Atherosclerotic Plaques, Hsin Yin Chuang, Huari Kou, Yue-Wern Huang, Hu Yang
Upar-Guided Dendrimer Gel Nanoparticles Reprogram Inflammation To Stabilize Atherosclerotic Plaques, Hsin Yin Chuang, Huari Kou, Yue-Wern Huang, Hu Yang
Biological Sciences Faculty Research & Creative Works
Atherosclerosis is characterized by lipid deposition, chronic inflammation, and apoptosis within the arterial wall, leading to plaque progression and instability. Current lipid-lowering therapies fail to fully address residual cardiovascular risk driven by local inflammation and cell death. Here, we report the development of uPA-functionalized, rapamycin-encapsulated dendrimer nanoparticles (G5PM-uPA/RA) that preferentially accumulate in urokinase plasminogen activator receptor (uPAR)-enriched atherosclerotic plaque, including macrophage- and apoptosis-rich lesion microenvironments. G5PM-uPA/RA was constructed by cross-linking reaction-enabled flash nanoprecipitation in a custom-made multi-inlet vortex mixer, followed by thiol-maleimide conjugation of uPA for uPAR-guided targeting. The nanoparticles demonstrated uniform morphology, favorable stability, and efficient rapamycin loading. In …
The Relationship Between Autistic Traits And Depression Among Self-Reporting College-Aged Adults, Tyler Michael Davis
The Relationship Between Autistic Traits And Depression Among Self-Reporting College-Aged Adults, Tyler Michael Davis
Walden Dissertations and Doctoral Studies
Depression is a prevalent mental health concern among emerging adults, particularly within college populations where academic, social, and developmental stressors are heightened. Autistic traits are increasingly understood as dimensional characteristics that exist across both clinical and non-clinical populations, yet their relationship to depressive symptoms in college-aged adults remains insufficiently understood. The purpose of this quantitative, cross-sectional correlational study was to examine the relationship between self-reported autistic traits and depressive symptoms among non-clinical adults aged 18 to 25. The study was grounded in Beck’s cognitive theory of depression and the diathesis–stress model, which posit that cognitive vulnerabilities interact with environmental stressors …
Polydopamine-Cu(Ii) Ions Functional Coatings On Zinc Wire: Surface Characterization And Degradation Behavior, Md Tanvir Hossain, H. R. Bakhsheshi-Rad, Bashir Ahamed, Erico Freitas, Bruce Lee, Jeremy Goldman, Jaroslaw Drelich
Polydopamine-Cu(Ii) Ions Functional Coatings On Zinc Wire: Surface Characterization And Degradation Behavior, Md Tanvir Hossain, H. R. Bakhsheshi-Rad, Bashir Ahamed, Erico Freitas, Bruce Lee, Jeremy Goldman, Jaroslaw Drelich
Michigan Tech Publications
Bioactive surface modification of degradable zinc (Zn) vascular implants is essential for controlling biodegradation and supporting vascular healing. Here, we describe the fabrication of polydopamine coating (PDA-Cu) containing up to ∼0.8 wt % Cu ions on Zn substrates using an immersion-assisted mussel-inspired polymerization strategy. The coating is composed of densely packed PDA-Cu nanospheres with diameters of ∼130-170 nm, exhibiting an even distribution of Cu ions within the PDA matrix via Cu-catechol coordination. Thirty-day leaching tests confirmed a steady release of 0.01-0.04 ppm of Cu ions per 5 days from Zn/PDA-Cu samples in Hank's balanced salt solution (HBSS). In vitro nitric …
Risk, Trust, And Medical Decision-Making: Perinatal Care Among Patients And Providers In South Florida, Isabella Viera
Risk, Trust, And Medical Decision-Making: Perinatal Care Among Patients And Providers In South Florida, Isabella Viera
Electronic Theses and Dissertations
This thesis examines how structural inequality shapes the experience of perinatal care among pregnant individuals and healthcare providers in South Florida, with particular attention to how risk is perceived and communicated, how trust between patients and providers is built or eroded, and how medical decision-making unfolds under conditions of structural vulnerability. Drawing on multi-sited ethnographic fieldwork conducted between October 2025 and April 2026, supplemented by a quantitative analysis of low-birth-weight rates across all 67 Florida counties, the project analyzes how risk is defined, communicated, and lived across community-based prenatal organizations, online educational platforms, and in-depth provider and patient interviews. The …
Avian Conservation And Translocations In The Mariana Archipelago, John V. Bender
Avian Conservation And Translocations In The Mariana Archipelago, John V. Bender
Dissertations
Island birds are among the world’s most imperiled vertebrates, and conservation translocations are increasingly used to combat threats, but their success depends on understanding the mechanics of whether and how to conduct translocations effectively. In the Commonwealth of the Northern Mariana Islands, native forest birds persist across a chain of islands where habitat degradation and loss, invasive species, and extreme weather create persistent conservation challenges. For my dissertation, I investigated how avian conservation programs and translocations can be planned, implemented, and improved under biological, logistical, and social uncertainty. For Chapter 1 I analyzed 15 years of avian translocations across seven …
Oregon State Rank Assessment For Bensonia (Bensoniella Oregana), Nora Dunkirk, Sue Vrilakas, Gabriel Campbell
Oregon State Rank Assessment For Bensonia (Bensoniella Oregana), Nora Dunkirk, Sue Vrilakas, Gabriel Campbell
Institute for Natural Resources Publications
Oregon state conservation status assessment for Bensonia (Bensoniella oregana) using NatureServe methodology, 2026.
Unbiased Avidity-Based Isolation Of Antigen-Specific T Cells, Amanda Montoya, Meredith L Frank, Peixin Jiang, Hui Nie, Minying Zhang, Emily Bontekoe, Jared K Slone, Ludovica L Posta, Sofia Rosy Caterina Sorice, Tina Cascone, Maura Gillison, Don L Gibbons, Jianjun Zhang, Eleonora Dondossola, Lydia Kavraki, Pamela L Wenzel, John V Heymach, Alexandre Reuben
Unbiased Avidity-Based Isolation Of Antigen-Specific T Cells, Amanda Montoya, Meredith L Frank, Peixin Jiang, Hui Nie, Minying Zhang, Emily Bontekoe, Jared K Slone, Ludovica L Posta, Sofia Rosy Caterina Sorice, Tina Cascone, Maura Gillison, Don L Gibbons, Jianjun Zhang, Eleonora Dondossola, Lydia Kavraki, Pamela L Wenzel, John V Heymach, Alexandre Reuben
The Brown Foundation: Institute of Molecular Medicine
Background: Cancer immunotherapies have significantly improved treatment efficacy and patient survival by exploiting antigen-specific T cells to eliminate cancer cells. However, current approaches for identifying and isolating antigen-specific T cells typically require prior knowledge of target antigens, limiting discovery, and reducing the ability to consistently detect rare tumor-reactive T cells. We therefore sought to develop an unbiased platform for the identification and enrichment of antigen-specific T cells using naturally processed and presented tumor antigens.
Methods: We developed ATTACH (Assessment of T cells Tethered to Antigen Class I Histocompatibility), a microfluidic platform that applies controlled shear stress and leverages tumor cells …