Digital Commons Network^™

Fluvastatin Enhances Healing Of Medication−Related Osteonecrosis Of The Jaw (Mronj) Via Macrophage Polarization In A Rat Mronj−Like Model, Moa Tazaki, Koma Sanda

Journal of Dental Sciences

No abstract provided.

The Utility Of 3d Nanostructured Polyglycolic Acid Fibers For Novel Bone Regenerative Medicine, Itsuki Hayashi, Sayaka Nakamura-Yamada

Journal of Dental Sciences

No abstract provided.

Dual Effects Of 1Α,25-Dihydroxyvitamin D3 On Inflammation And Osteogenic Differentiation In Lipopolysaccharide-Stimulated Dental Pulp Stem Cells, Seong Eun Song, Giyoung Yun

Journal of Dental Sciences

No abstract provided.

A Scientometric Study On Research Trends And Characteristics Of Diabetes-Associated Periodontal Disease, Man Yang, Wenxiao Shi, Yan Xiong, Mengjie Chen, Linjun Shi, Huiwen Chen

Journal of Dental Sciences

Background/purpose: Substantial evidence demonstrates that diabetes is associated with periodontal disease. The purpose of this study was to analyze the scientometric characteristics and research trends of diabetes-associated periodontal disease (DAPD).

Materials and methods: All the papers on DAPD were comprehensively retrieved from the Scopus database. The years of publication were divided into before 2019 and after 2019 in the analysis of research trends.

Results: There were 2466 papers on DAPD, with total citations of 73,553 and the h index of 120. The trend of clinical investigations has highlighted periodontal examination, bone development, micro-computed tomography, tooth brushing, cardiovascular …

Socket Regeneration Using A Composite Regenerative Matrix: A Simplified Clinical Workflow With A 5-Year Follow-Up, Chi-Shan Tseng, Ming-Ko Li, Cheng-Chia Yu

Journal of Dental Sciences

Alveolar ridge remodeling following tooth extraction remains a clinical challenge in implant dentistry, particularly in the esthetic zone where dimensional stability of hard and soft tissues is critical for subsequent implant placement. This short communication describes a simplified clinical workflow for socket regeneration using a composite regenerative matrix (CRM) composed of concentrated growth factor (CGF), autologous fibrinogen glue (AFG), and freeze-dried bone allograft (FDBA). The technique was applied following atraumatic extraction in a single maxillary esthetic-zone case. Clinical and radiographic observations were documented for up to 5 years. Healing was uneventful, and cone-beam computed tomography performed at 3 months demonstrated …

Negative Pulp Sensibility Responses Do Not Necessarily Indicate Pulp Necrosis In Teeth Associated With Cystic Or Cyst-Like Lesions, Le Lu, Xiang Zhang, Ya Shen, He Liu

Journal of Dental Sciences

No abstract provided.

The Story Of A Taiwanese Female Student Studying Dentistry In Japan During The Japanese Colonial Period: An Oral History By Dr. Yueh-E Wu, Feng-Chou Cheng, Mei-Huei Li, Chien-Chi Chu, Chun-Pin Chiang

Journal of Dental Sciences

No abstract provided.

Avian Mesenchymal Stem Cell-Derived Extracellular Vesicle Alleviates The Porphyromonas Gingivalis Lipopolysaccharide Induced Gingival Fibroblast Senescence, Han-Chieh Yang, Earl Fu, Yu-Tang Chin, Ju-Sheng Shieh, Wei-Ni Lin, Cheng-Yang Chiang

Journal of Dental Sciences

Background/purpose: We have shown that extracellular vesicles (EVs) derived from Polygonum multiflorum extract bio-pulsed avian mesenchymal stem cells exhibit anti-inflammatory and proliferation-promoting properties. A significant association has been identified between periodontitis, higher biological age, and accelerated biological aging. This study investigated whether the bio-pulsed EVs could alleviate Porphyromonas gingivalis lipopolysaccharide (Pg-LPS)-induced senescence in human gingival fibroblasts (hGFs).

Materials and methods: Pg-LPS was used to induce cellular inflammation and senescence in hGFs. The cytokines IL-1β, IL-6, and TNF-α were assessed by quantitative polymerase chain reaction (qPCR), and cytotoxicity was assessed with MTS. Senescence after repeated stimulations was evaluated …

Developing An Automated Cytology-Based Dna Ploidy Test For Detecting Oral Squamous Cell Carcinoma In Taiwan: A Feasibility Study, Pei Hsuan Lu, Kelly Yi Ping Liu, Julia Yu Fong Chang, Long‐Wei Lin, Hao-Hung Chang, Chen-Han Chang, Shang Hsuan Peng, Catherine F. Poh

Journal of Dental Sciences

Background/purpose: Oral squamous cell carcinoma (OSCC) remains a global health issue, particularly in underserved settings such Taiwan where access to early diagnosis is limited. DNA ploidy-based oral cytology has shown strong diagnostic performance, but its applicability across populations require evaluation.

Materials and methods: In this prospective feasibility study, brush and oral rinse samples were collected from patients with OSCC, low-grade dysplasia, reactive lesions, and normal mucosa. DNA cytometry was performed to quantify ploidy profiles. Diagnostic performance was evaluated using published algorithm and cohort-specific thresholds derived from stratified 10-fold cross-validation.

Results: A total of 118 patients were included (50 OSCC, 41 …

Anemia, Hematinic Deficiencies, Hyperhomocysteinemia, And Serum Gastric Parietal Cell Antibody Positivity In Recurrent Aphthous Stomatitis Patients With Vitamin B12 Deficiency, Yi-Ping Wang, Yi-Pang Lee, Yu-Hsueh Wu, Julia Yu-Fong Chang, Andy Sun, Chun-Pin Chiang

Journal of Dental Sciences

Background/purpose: Our previous study found that 30 of 430 recurrent aphthous stomatitis (RAS) patients have vitamin B12 deficiency. This study assessed whether the vitamin B12-deficient RAS (B12D/RAS) patients had significantly higher frequencies of anemia, hematinic deficiencies, hyperhomocysteinemia, and serum gastric parietal cell antibody (GPCA) positivity than healthy control subjects and evaluated whether all B12D/RAS patients had pernicious anemia (PA).

Materials and methods: The blood hemoglobin (Hb) and serum iron, vitamin B12, folic acid, homocysteine, and GPCA levels in 430 RAS patients including 30 B12D/RAS patients and 430 healthy control subjects were measured and compared.

Results: We found that 30 B12D/RAS …

A Bibliometric Evolution Of Molecular Mechanisms And Emerging Research Trends Of Therapeutic Resistance In Oral Cancer, Chu-Yen Chien, Ying-Chen Chen, Jia-Rong Wu, Hsu-Hua Chu, Yi-Jen Hung, Yi-Shing Shieh

Journal of Dental Sciences

Background/purpose: Drug resistance has served as a primary determinant of treatment failure and disease recurrence in oral cancer. The present study aimed to evaluate the global research landscape of drug resistance mechanisms in oral cancer by integrating bibliometric mapping with bioinformatic validation.

Materials and methods: A comprehensive dataset of 1660 publications was retrieved from the Scopus database. Bibliometric analyses were performed using Biblioshiny and VOSviewer to evaluate publication trends, collaborative networks, and thematic evolution. To bridge research trends with biological mechanisms, 1469 genes associated with oral cancer and drug resistance were extracted from the GeneCards database and subjected to functional …

Effects Of Low-Level Er:Yag Laser Irradiation On Endothelial Angiogenic Responses In Diabetes: An In Vitro Study, Bruna Akinaga Moreira, Koji Mizutani, Natsumi Saito, Lu Chen, Shu Takemura, Keita Nakagawa, Yujin Ohsugi, Taichen Lin, Takanori Iwata, Akira Aoki

Journal of Dental Sciences

Background/purpose: Low-level Er:YAG laser irradiation has been reported to promote angiogenesis-related endothelial responses and enhance periodontal tissue healing. However, its effects on angiogenesis under hyperglycemic conditions have not been investigated. This study aimed to investigate whether low-level Er:YAG laser irradiation enhances the angiogenic response under hyperglycemic conditions.

Materials and methods: Human umbilical vein endothelial cells (HUVECs) were cultured in control-glucose (CG, 5.5 mM) or high-glucose (HG, 25 mM) medium and subjected to low-level laser irradiation (LLLI) using an Er:YAG laser at 10 Hz and 30 mJ/pulse for 30 s under two pulse-widths. Angiogenesis-related cellular functions were evaluated by assessing cell …

Blood Vessels Expressing Cd31 And Endomucin Contribute To The Formation Of New Hard Tissue Via Osterix-Expressing Cells During The Healing Process Of Pulp Revascularization In Mice, Takatoshi Ikarashi, Keisuke Mitomo, Kentaro Tashiro, Mayuka Haketa, Toshihide Mizoguchi, Akira Yamaguchi, Takashi Muramatsu

Journal of Dental Sciences

Background/purpose: Niche for osteogenesis forms near specialized blood vessels that highly express both CD31 and endomucin (Emcn). However, localization of the vessels remains unknown during wound healing process after pulp revascularization. We investigated the localization of both CD31 and Emcn-expressing vessels and osterix (Osx)-expressing cells after pulp revascularization. Materials and methods: Five-week-old male C57BL/6J mice underwent pulp revascularization in the mesial root canal of the right first maxillary molar. The mice were sacrificed at 1 hour, 7 days, 14 days, and 21 days post-surgery, and immunofluorescence for CD31, Emcn, and Osx was performed. Additionally, calcein labeling was used to visualize …

Physico-Mechanical Properties And Streptococcus Mutans Biofilm Formation Of Bio‑Based Flexible Crowns Versus Stainless Steel And Zirconia Crowns For Pediatric Full-Coverage Restorations, Tzu-Yu Peng, Szu-Yu Lai, Yan-An Wu, Ya-Ting Yang, Yuichi Mine, Huei-Yu Huang

Journal of Dental Sciences

Background/purpose: Bio‑based flexible (BFX) have recently emerged as a potential alternative to conventional pediatric full‑coverage restorations, but evidence regarding their properties and microbial behavior remains limited. Thus, this study aimed to evaluate the physico-mechanical properties and microbial behavior of BFX crown compared with stainless steel crowns (SSC) and zirconia crowns (ZRC) for pediatric full-coverage restorations.

Materials and methods: Mandibular primary molar crowns from BFX, SSC, and ZRC were assessed. Weight was measured using an electronic balance, and surface roughness and topography were evaluated using a stylus profilometer and atomic force microscopy. The arithmetic mean roughness (Ra) and root mean square …

Anemia, Hematinic Deficiencies, And Hyperhomocysteinemia In Gastric Parietal Cell Antibody-Positive And -Negative Recurrent Aphthous Stomatitis Patients, Yu-Hsueh Wu, Yi-Ping Wang, Yi-Pang Lee, Julia Yu-Fong Chang, Andy Sun, Chun-Pin Chiang

Journal of Dental Sciences

Background/purpose: Our previous study found the serum gastric parietal cell antibody (GPCA) positivity in 12.8% of 430 recurrent aphthous stomatitis (RAS) patients. This study assessed whether GPCA-positive RAS (GPCA⁺RAS) patients had significantly higher frequencies of macrocytosis, anemia, hematinic deficiencies, and hyperhomocysteinemia than healthy control subjects or GPCA-negative RAS (GPCAˉRAS) patients.

Materials and methods: The mean corpuscular volume, blood hemoglobin (Hb), and serum iron, vitamin B12, folic acid, homocysteine, and GPCA levels were measured and compared between any two of three groups of 55 GPCA⁺RAS patients, 375 GPCAˉRAS patients, and 430 healthy control subjects.

Results: We found …

Sesamin Suppresses High Glucose-Induced Cyclooxygenase-2 And Prostaglandin E2 Production Through Toll-Like Receptor 2-Dependent Oxidative Stress In Human Gingival Fibroblasts, Ya-Lun Li, Yu-Chia Hsu, Fang-Yu Fan, Yung-Li Wang, Yang-Che Wu, Sin-Yu Li, Thi Thuy Tien Vo, Yu-Chia Chang, Yin-Tzer Shih, I-Ta Lee

Journal of Dental Sciences

Background/purpose: Hyperglycemia contributes to periodontal inflammation through oxidative stress and inflammatory mediator production in gingival fibroblasts. Sesamin, a major lignan from sesame, has antioxidant and anti-inflammatory properties. This study investigated whether sesamin attenuates high glucose (HG)-induced cyclooxygenase-2 (COX-2) expression and prostaglandin E₂ (PGE₂) production in human gingival fibroblasts (HGFs), and examined the involvement of Toll-like receptor 2 (TLR2)-dependent oxidative signaling.

Materials and methods: HGFs were exposed to HG in the presence or absence of sesamin. Cell viability, reactive oxygen species (ROS) generation, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, COX-2 expression, and PGE₂ production were evaluated. …

Anemia, Hematinic Deficiencies, Hyperhomocysteinemia, And Serum Gastric Parietal Cell Antibody Positivity In Recurrent Aphthous Stomatitis Patients With Iron Deficiency, Yi-Ping Wang, Yu-Hsueh Wu, Yi-Pang Lee, Julia Yu-Fong Chang, Andy Sun, Chun-Pin Chiang

Journal of Dental Sciences

Background/purpose: Our previous study found that 101 of 430 recurrent aphthous stomatitis (RAS) patients have iron deficiency (ID). This study assessed whether all RAS patients with ID (so-called ID/RAS patients) had iron deficiency anemia (IDA) and evaluated whether the ID/RAS patients had significantly higher frequencies of anemia, hematinic deficiencies, hyperhomocysteinemia, and serum gastric parietal cell antibody (GPCA) positivity than healthy control subjects.

Materials and methods: The blood hemoglobin (Hb) and serum iron, vitamin B12, folic acid, homocysteine, and GPCA levels in 101 ID/RAS patients and 430 healthy control subjects were measured and compared.

Results: We found that …

Routine Dental Scaling And Reduced Risk Of Serious Infections In Individuals With Moderate-To-Severe Cerebral Palsy: A Nationwide Population-Based Cohort Study, Li-Wei Ou-Yang, Yu-Tung Huang, Ya-Ting Chang, Hsin-Mao Hsu, Shih-Hao Chang, Chia-Ling Wu, Tsung-Chieh Yao

Journal of Dental Sciences

Background/ purpose: Individuals with moderate-to-severe cerebral palsy (CP) are highly susceptible to serious infections. Oral biofilms may serve as reservoirs for pathogenic microorganisms, yet the impact of professional oral healthcare on systemic infection risk in this population remains unclear. This study investigated the association between routine dental scaling and the risk of serious infections and infection-related mortality among individuals with moderate-to-severe CP.

Materials and methods: This nationwide, population-based cohort study used data from Taiwan’s National Health Insurance Research Database and National Death Registry (2009–2019). Individuals with moderate-to-severe CP were categorized into a routine dental scaling group (≥1 scaling annually for …

Artificial Intelligence Assisted Multitask Framework For Dental Detection And Root Canal Filling Assessment On Periapical Radiographs, Chia-Lin Hsu, Li Wei Ou-Yang, Chih-Hao Chang, Yuan-Jin Lin, Po-Lin Huang, Kai-Syun Chiu, Heng-Chia Chen, Tsung-Yi Chen, Jia-Ching Wang, Wei-Chen Tu, Patricia Angela R. Abu

Journal of Dental Sciences

Background/purpose: Accurate evaluation of root canal filling (RCF) quality is paramount for successful endodontic outcomes. However, conventional radiographic interpretation is often subject to subjective variability. This study developed an automated, multitask artificial intelligence (AI) framework using YOLO11 and advanced image pre-processing to identify multiple dental structures and provide quantitative assessments of RCF quality on periapical radiographs.

Materials and methods: We proposed an image enhancement pipeline, which integrate intensity normalization, white top-hat transform, and linear contrast stretching to refine dental contours for training the YOLO11 multitask model. The framework simultaneously detected primary, permanent, dental crowns, and RCFs. Quantitative metrics, including …

Do Anatomy, Physiology And Biochemistry Subject Scores Predict Future Academic Performance? A Longitudinal Analysis Of Undergraduate Dental Students Across All Professional Years., Shahid Akhtar Akhund, Hassan Shaibah

Journal of Dental Sciences

Background/purpose: Basic sciences subjects including anatomy, physiology and biochemistry form the foundation of the Bachelor of Dental Surgery (BDS) programme. However, empirical evidence linking the basic science academic performance to sustained academic achievement across subsequent clinical years remains limited. This is true for low and medium sized economies like Pakistan.

Materials and methods: A retrospective, quantitative, and longitudinal cohort study was conducted.  The deidentified data from a representative dental college was used. Basic science examination scores from the 1^st professional year examination served as predictor variables. The examination scores across 14 subjects served as outcome variables. Pearson product-moment correlations …

Deer Antler Tip Cell Culture Supernatant Restores Endothelial Barrier Function And Angiotensin Converting Enzyme 2/Endothelial Nitric Oxide Synthase Nitric Oxide Signaling Impaired By Porphyromonas Gingivalis Lipopolysaccharide, Ming-Shou Hsieh, Ya-Ting Yang, Chin-Sheng Huang, Ting-Yi Renn, Chuen-Kai Wang, Chia-Chen Hsu, Wei Jen Chang, Yu-Sheng Chang

Journal of Dental Sciences

Background/purpose:

Periodontal inflammation contributes to endothelial dysfunction, but effective biologically active strategies for vascular protection remain limited. This study investigated whether deer antler tip cell culture supernatant could rescue Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) induced endothelial injury and restore endothelial homeostasis.

Materials and methods:

Velvet antler tissues from the tip, shaft, and burr regions were examined histologically and used for primary cell isolation. Human umbilical vein endothelial cells were exposed to Pg-LPS or indirectly co-cultured with LPS-stimulated THP-1-derived macrophages. Deer antler tip cell culture supernatant was subsequently applied to these endothelial inflammatory models, and its protective effects were …

Hollow Fiber Assay Microtube Array Membrane (Mtam-Hfa) For Personalized Anticancer Drug Screening In Selection Of Concurrent Chemoradiotherapy In Oral Squamous Cell Carcinoma, Shan-Ju Chou, Po-Chih Hsu, Chee Ho Chew, Wan-Ting Huang, Amanda Lin Chen, Chen-Yin Yong, Chia-Che Wu, Chia-Lun Chang, Chien-Chung Chen, Kuan-Chou Lin

Journal of Dental Sciences

Background/purpose: In Asia, oral squamous cell carcinoma (OSCC) are on the rise in part due to the consumption of betel nut in several regions. This clinical case study evaluated the application of the microtube array membrane-hollow fiber assay (MTAM-HFA) that was previously clinically demonstrated in the context of the selection of chemotheraputic agents as part of the treatment of concurrent chemoradiotherapy (CCRT) in OSCC.

Materials and methods: In this double-blind cohort study, surgical samples from OSCC patients were retrieved and sent for screening of tumor response against a panel of chemotherapeutic agents (5-fluorouracil (5-FU), cisplatin, carboplatin and docetaxel). …

Heat Shock Factor 1 Promotes Cell Migration And Predicts Poor Prognosis In Oral Squamous Cell Carcinoma, Fang-Yu Wu, Hui-Hsin Ko, Wen-Ren Lin, Shih-Jung Cheng

Journal of Dental Sciences

Background/purpose

Heat shock factor 1 (HSF1) regulates heat shock proteins and stress-responsive genes and has been implicated in tumor progression and poor prognosis in several malignancies. However, the clinicopathological significance and biological role of HSF1 in oral squamous cell carcinoma (OSCC) remain unclear. This study investigated HSF1 expression in OSCC tissues, its association with clinicopathological parameters and survival, and its functional role in OSCC cell migration.

Materials and methods

HSF1 expression was evaluated by immunohistochemical staining in 80 OSCC specimens and 20 normal oral mucosa samples. HSF1 immunoreactivity was quantified using the labeling index (LI). Associations between HSF1 LI and …

Runt-Related Transcription Factor 2 Transcriptionally Represses Sirtuin 1 To Regulate Osteoclastogenesis And Tooth Eruption, Jie Li, Changqing Yan, Xinyi Sun, Chenying Zhang, Xiaozhe Wang, Yang Liu, Shuguo Zheng

Journal of Dental Sciences

Background/purpose: Cleidocranial dysplasia (CCD), caused by Runt-related transcription factor 2 (RUNX2) haploinsufficiency, features delayed tooth eruption. Although impaired osteoclastogenesis contributes to eruption failure, the direct molecular mechanisms linking RUNX2 deficiency to osteoclast dysfunction remain elusive. Sirtuin 1 (SIRT1) is a critical regulator of osteoclastogenesis; however, whether it serves as a downstream effector of RUNX2 remains unexplored. This study aimed to elucidate the mechanistic link between RUNX2 and SIRT1 in regulating osteoclast differentiation and tooth eruption.

Materials and methods: Tooth eruption and osteoclast differentiation were assessed by micro-computed tomography and histological analysis in Runx2^+/- mice. Chromatin immunoprecipitation quantitative polymerase chain …

Erased From The Permanent Record: Data Collection Practices And Non-Binary Student Experiences In School, Ashley Carey, Peter Piazza

Education Faculty Publications

State education data systems often undercount non-binary students, constraining schools’ ability to support them. This study examines the scope and implications of non-binary erasure in school data collection. Drawing on survey responses from nearly 13,000students across seven Massachusetts districts, we find that current practices substantially undercount non-binary students, who also report less positive experiences of school culture, including student–teacher relationships, belonging, and emotional safety. Although inclusive data could guide efforts to create more supportive environments, districts face growing political resistance from parent organizations aligned with the White Christian Nationalist movement. These groups frame inclusivity as a threat to parental rights. …