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2026

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Rewilding The Coral Reefs, Magali Rodriguez, Luciana Alvillar Jan 2026

Rewilding The Coral Reefs, Magali Rodriguez, Luciana Alvillar

Posters - 2026

Coral reefs are among the most valuable ecosystems globally, contributing an estimated $10 trillion annually and supporting hundreds of millions of people through food, livelihoods, and coastal protection. (NOAA 2026) They also provide critical habitat for diverse marine species. However, reefs are rapidly declining due to rising ocean temperatures, acidification, pollution, invasive species, and physical disturbances. Globally, 30–50% of coral reefs have already been lost(NOAA 2026), and without intervention, many may face extinction by the end of the century. Rewilding and restoration efforts have become essential strategies to address this crisis. Initiatives such as NOAA’s Coral Reef Conservation Program highlight …


Urban Trees In The Urban Hydrology Cycle, Jorge R. Vera Jan 2026

Urban Trees In The Urban Hydrology Cycle, Jorge R. Vera

Theses, Dissertations and Capstones

In urban forestry, trees provide numerous economic, ecological, and aesthetic benefits to the surrounding community. One of their key roles is intercepting stormwater runoff, reducing flooding risk, and improving water quality. Chapter 1 of this thesis characterized the ecological and economic contributions of urban trees on the Marshall University campus and provides guidance on their management and conservation in the future. Chapter 2 examined how urban tree species modify the urban hydrology cycle by altering the volume and timing of precipitation delivery. In Chapter 1, we inventoried 114 acres of Marshall University's main campus in Huntington, WV, from June 28 …


Maxillary Anterior Teeth Replacement With Immediate Dental Implants Utilizing Vestibular Socket Therapy Versus Ice Cream Cone Technique, Mohamed Faisal Abd El Raorf Shoeib, Mohammed Abdel-Monem Tawfik, Mohammed Talaat Salem, Ayman Ahmed Mustafa Yaseen Jan 2026

Maxillary Anterior Teeth Replacement With Immediate Dental Implants Utilizing Vestibular Socket Therapy Versus Ice Cream Cone Technique, Mohamed Faisal Abd El Raorf Shoeib, Mohammed Abdel-Monem Tawfik, Mohammed Talaat Salem, Ayman Ahmed Mustafa Yaseen

Mansoura Journal of Dentistry

Objective: This study intended to evaluate changes, clinically and radiographically, associated with the immediate placement of dental implants in the maxillary anterior esthetic zone, comparing vestibular socket therapy with the ice cream cone technique. Materials and Methods: The current research included sixteen individuals requiring the replacement of a non-restorable tooth in the maxillary anterior aesthetic zone with an implant. Patients were divided into two groups of equal numbers. In the first group, eight implants were immediately placed utilizing the ice cream cone technique, whereas in the second group, eight implants were immediately inserted using vestibular socket therapy into the maxillary …


Ultrasound-Guided Vs Conventional Intraarticular Injection Of Hyaluronic Acid For Management Of Temporomandibular Joint Internal Derangement: A Randomized Controlled Trial, Muhanad Almufarrih, Muhammad Al-Hawari, Ayman Yaseen, Sally Abdelsameaa Jan 2026

Ultrasound-Guided Vs Conventional Intraarticular Injection Of Hyaluronic Acid For Management Of Temporomandibular Joint Internal Derangement: A Randomized Controlled Trial, Muhanad Almufarrih, Muhammad Al-Hawari, Ayman Yaseen, Sally Abdelsameaa

Mansoura Journal of Dentistry

Objective: This study aimed to evaluate the clinical efficacy of using ultrasound guidance during intra-articular injection of hyaluronic acid (HA) in the management of patients with TMJ internal derangement. Material/subjects and Methods: Forty patients complaining of anterior disc displacement with reduction (DDwR) not responsive to conservative treatment were enrolled in this study. The diagnosis was based on the current update of diagnostic criteria for temporomandibular disorders (TMDs). Intra-articular injection of Hyaluronic Acid guided by Ultrasound in the first group and Intra-articular Injection (IAI) of HA conventional way in the second group were performed. Articular pain was evaluated using the visual …


Extended Platelet Rich Fibrin In Lateral Maxillary Sinus Lifting With Simultaneous Implant Placement, Ammar Radwan Samman, Heba Abo-Elfetouh Elsheikh, Walid Abd El-Ghafar Al-Zordk, Noha Ahmed Mansour Jan 2026

Extended Platelet Rich Fibrin In Lateral Maxillary Sinus Lifting With Simultaneous Implant Placement, Ammar Radwan Samman, Heba Abo-Elfetouh Elsheikh, Walid Abd El-Ghafar Al-Zordk, Noha Ahmed Mansour

Mansoura Journal of Dentistry

Objective: This study aimed to investigate the influence of extended platelet-rich fibrin (e-PRF) when used in lateral maxillary sinus floor augmentation (MSFA), with particular emphasis on implant stability and peri-implant marginal bone loss.

Materials and Methods: Thirteen patients presenting with posterior maxillary tooth loss and a residual bone height of 3–5 mm underwent lateral sinus floor augmentation with concomitant implant placement. A total of 16 implant sites were included and equally allocated into two groups: Group I (n = 8), in which an allograft was applied and covered using a pericardium membrane, and Group II (n = 8), in which …


Effect Of Biodentine And Treated Dentine Matrix On Viability And Proliferation Of Human Dental Pulp Stem Cells, Nada Barakat, Rana El-Qashty, Mohamad E. Helal Jan 2026

Effect Of Biodentine And Treated Dentine Matrix On Viability And Proliferation Of Human Dental Pulp Stem Cells, Nada Barakat, Rana El-Qashty, Mohamad E. Helal

Mansoura Journal of Dentistry

Objective: The present study aimed to assess the dose-dependent effect of Biodentine (BD) and human-treated dentin matrix (hTDM) on the proliferation of human dental pulp stem cells (hDPSCs).

Materials and methods: hTDM was prepared and characterized. The concentration of proteins released from BD and hTDM extracts were quantified using the bicinchoninic acid (BCA) assay after 3 and 7 days. hDPSCs were successfully isolated and characterized, then treated with various concentrations of BD extracts (20, 2, and 0.2 mg/mL) and hTDM extracts (100%, 80%, 60%, 40%, 20%, and 10%). Cell viability was determined after 7 days of incubation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium …


An Attempt For In Situ Bio-Root Regeneration By Using Treated Dentin Matrix And Dental Pulp Stem Cells In Albino Rats, Amani Mohamad, Ahmad A. Emam, Eman Hany, Mohamed Abdulrahman Jan 2026

An Attempt For In Situ Bio-Root Regeneration By Using Treated Dentin Matrix And Dental Pulp Stem Cells In Albino Rats, Amani Mohamad, Ahmad A. Emam, Eman Hany, Mohamed Abdulrahman

Mansoura Journal of Dentistry

Objectives: This study was conducted to prepare and characterize root-shaped treated dentin matrix (TDM) scaffolds, and to evaluate their efficacy together with seeded dental pulp stem cells (DPSCs) as potential bio-roots in extraction sockets of rat models.

Materials and Methods: Root-shaped TDM specimens were prepared and characterized. Following extraction of maxillary incisors, the alveolar sockets were implanted with TDM segments measuring 8 mm in length, either alone (TDM group) or seeded with dental pulp stem cells (TDM/DPSCs group). Animals were euthanized at two and six weeks post-implantation, specimens were processed for histological, histochemical and immunohistochemical analyses.

Results: TDM group at …


In Utero Drug Exposure (Iude): Fact Sheet, The Rees-Jones Center For Foster Care Excellence Jan 2026

In Utero Drug Exposure (Iude): Fact Sheet, The Rees-Jones Center For Foster Care Excellence

Infographics and One-Pagers

This one-pager provides an overview of Intrauterine Drug Exposure (IUDE), outlining its clinical definitions and the short-term and long-term developmental impacts on infants.

For questions about this publication or others, please email [email protected]

childrens.com/fostercare


Intensive Longitudinal Methods: Toward A Psychological Science Of Daily Life, Jean-Philippe Laurenceau, Ana M. Digiovanni, Niall Bolger Jan 2026

Intensive Longitudinal Methods: Toward A Psychological Science Of Daily Life, Jean-Philippe Laurenceau, Ana M. Digiovanni, Niall Bolger

Department of Psychology Faculty Scholarship and Creative Works

Intensive longitudinal methods (ILMs) represent a class of longitudinal designs used to understand the flow of people's thoughts, feelings, physiology, and behaviors in their natural settings. This term encompasses daily diaries, experience sampling, ecological momentary assessment, ambulatory assessment, and related methods. Research on ILMs has grown exponentially, evolving into a core approach that complements more traditional designs. This article builds on this journal's first review on this topic, published in 2003. In the quarter-century since, there have been marked advances in design, technology, and statistical modeling. Three core ideas permeate this review: To build adequate theories of psychological functioning in …


A Farewell To Space Arms Control, Eytan Tepper, James B. Romano Jan 2026

A Farewell To Space Arms Control, Eytan Tepper, James B. Romano

Saint Louis University Law Journal

This paper examines the nearly seven decades of attempts to adopt multilateral rules restricting space arms race and limit military uses of outer space. The paper presents the early success to introduce, as part of the 1967 Outer Space Treaty, basic key limitations, notably a ban on weapons of mass destruction and offensive military uses of celestial bodies, and the subsequent failure to expand these basic rules. Indeed, despite forty-five years since the early 1980s of work in various streams and frameworks attempting to prevent a space arms race, and despite many countries’ proclamations supporting space arms control, no meaningful …


Birthing Inequality: How U.S. Midwifery Laws Perpetuate Racial And Socioeconomic Disparities, Miranda Martin Jan 2026

Birthing Inequality: How U.S. Midwifery Laws Perpetuate Racial And Socioeconomic Disparities, Miranda Martin

Saint Louis University Law Journal

“In our nation . . . more women are facing death because of childbirth than in any other developed nation.” “Black women are three times more likely to die in connection with childbirth . . . Native women twice as likely . . . [and] rural women are one and a half times more likely to die,” warned Vice President Kamala Harris, emphasizing the country’s maternal health crisis. Despite efforts to improve maternal health, access to safe and effective care remains uneven. In the United States, there is confusion among legal and healthcare professionals regarding the different types of midwives …


Filling The Network Gap In Research Ethics: Analyzing Ethical Issues At Scale In Big Team Science, Susan Wolf, Gillian H. Roehrig, Timothy L. Pruett, Korkut Uygun, Claire Colby Mcvan, Evelyn Brister, Shawneequa L. Callier, Alexander M. Capron, James F. Childress, Rosario Isasi, Andrew D. Maynard, Kenneth A. Oye, Paul B. Thompson, Terrance R. Tiersch Jan 2026

Filling The Network Gap In Research Ethics: Analyzing Ethical Issues At Scale In Big Team Science, Susan Wolf, Gillian H. Roehrig, Timothy L. Pruett, Korkut Uygun, Claire Colby Mcvan, Evelyn Brister, Shawneequa L. Callier, Alexander M. Capron, James F. Childress, Rosario Isasi, Andrew D. Maynard, Kenneth A. Oye, Paul B. Thompson, Terrance R. Tiersch

Articles

Scientific research increasingly involves large, multidisciplinary teams networked across multiple institutions to develop new technologies. Despite the rise of complex research networks and big team science, there has been too little analysis to date of the ethical challenges facing these networks. The extensive literature on the ethical issues confronting individual researchers and small teams (the microlevel) and on the larger societal challenges flowing from research and new technology (the macrolevel) leaves a troubling gap in between, at the mesolevel of the research network involved in big team science. Yet the ability of complex networks to conduct research ethically—which is essential …


Ms-Ls2-1: Resource Availability, Maddie Epler, Eden Jasicki Jan 2026

Ms-Ls2-1: Resource Availability, Maddie Epler, Eden Jasicki

5th Grade

No abstract provided.


Beyond The Bars: Reimagining The Dc Correctional System As The Frontline For Hepatitis C Elimination, Rahimatu Jalloh, Amber Munoz, Riyan Lee, Kristen Martinez, Malia Allen, Gerard Lin Jan 2026

Beyond The Bars: Reimagining The Dc Correctional System As The Frontline For Hepatitis C Elimination, Rahimatu Jalloh, Amber Munoz, Riyan Lee, Kristen Martinez, Malia Allen, Gerard Lin

DC Health and Academic Prep Program (DC HAPP)

Hepatitis C is a major public health concern among incarcerated populations, who are infected at rates approximately 10 times higher than the general U.S. population. In Washington, D.C., an estimated 12,700 people are living with hepatitis C, and the District has one of the highest HCV prevalence and mortality rates in the nation. Although hepatitis C is highly curable, many incarcerated individuals do not receive treatment despite having enough time to complete therapy while incarcerated.

This project proposes a comprehensive hepatitis C program for the D.C. prison system that addresses HCV through prevention, intake screening, treatment during incarceration, and post-release …


Shaping The Future: Emerging Technologies And Their Role In Industry 4.0 And Beyond, Liuliu Qin Jan 2026

Shaping The Future: Emerging Technologies And Their Role In Industry 4.0 And Beyond, Liuliu Qin

Information Technology & Decision Sciences Faculty Publications

This paper provides a comprehensive review of emerging technologies driving the transition from Industry 4.0 to Industry 5.0. It examines the foundational concepts and pillars of Industry 4.0 and explores the transformative roles of Artificial Intelligence (AI), Extended Reality (XR), Collaborative Cobots (Cobots), Brain–Computer Interfaces (BCIs), quantum technologies, and next-generation connectivity (5G/6G). By integrating technological, human-centric, and sustainability perspectives, the study outlines how these emerging technologies reshape industrial systems and enable intelligent, adaptive, and inclusive futures.


Urban Heat Dynamics: Comparing Thermal Regulation Of Patios And Green Roofs, Charlotte Gordy, Bishoy S. Sami, Darlene Panvini Jan 2026

Urban Heat Dynamics: Comparing Thermal Regulation Of Patios And Green Roofs, Charlotte Gordy, Bishoy S. Sami, Darlene Panvini

SPARK Symposium Presentations

Urban environments are characterized by elevated temperatures caused by the Urban Heat Island effect, an ecological phenomenon distinguished by the abundance of artificial surfaces such as concrete and tile that absorb heat. This study investigates how surface temperatures differ between traditional patios and green roof gardens, and how these differences vary under sunny and shady conditions. We hypothesize that green roof gardens will exhibit significantly lower surface temperatures than paved surfaces, particularly on sunny days.

To test this hypothesis, we will select two to three buildings on campus that contain both traditional patio surfaces and green roof gardens. Using an …


A New Framework For Drug Pricing Law And Policy, Rachel E. Sachs Jan 2026

A New Framework For Drug Pricing Law And Policy, Rachel E. Sachs

Indiana Law Journal

Scholars and policymakers have expressed concern about the impact of high prescription drug costs on patients and healthcare budgets. This Article presents a new theoretical framework for evaluating both the problem to be addressed by drug pricing reforms as well as the efficacy of potential solutions. In seeking to solve “the drug pricing problem,” our legal system has given primacy to competition rather than regulation to drive down drug prices. This Article disaggregates the broader concept of drug competition into four steps, each of which must be addressed and each of which elevates different institutional actors to key roles. In …


When Death Is Not Binary, Teneille R. Brown Jan 2026

When Death Is Not Binary, Teneille R. Brown

Indiana Law Journal

There are few dichotomies more recognized than the one between life and death. Given the many legal rights that pivot on this distinction, one would think that the law has exceptionally clear standards for diagnosing death. It may surprise you then to learn that the line between legal life and death is no longer so obvious. That is, in a growing number of cases, there is now disagreement about whether someone is legally dead or alive. This is due to two things: the development of medical devices that can breathe for patients with severe brain injuries, and neuroimaging technology that …


Testing Spatial Memory And Associative Learning In Planaria With Chronic Nicotine Usage, Gregory Smith, Jessica Toledo-Franco Jan 2026

Testing Spatial Memory And Associative Learning In Planaria With Chronic Nicotine Usage, Gregory Smith, Jessica Toledo-Franco

SPARK Symposium Presentations

Nicotine is a psychoactive substance, commonly delivered through e-cigarettes. In the short-term, nicotine can cause an individual to feel enhancements in attentiveness, while also reducing stress or withdrawal symptoms. Withdrawal generally refers to the phase after quitting or reducing the use of a substance; however, in nicotine, these symptoms can be seen as early as the first usage. Unlike most studies that have focused on mammals as the subject, the goal of this study is to investigate how nicotine addiction and withdrawal influences learning and memory through Dugesia dortocephala in T and Y mazes. As the concentration of nicotine increases, …


Impacts Of Native And Nonnative Plant Species On Avian Community Diversity, Matty John Mackay, Steven Van Dang, Brandon Lee Corn Jan 2026

Impacts Of Native And Nonnative Plant Species On Avian Community Diversity, Matty John Mackay, Steven Van Dang, Brandon Lee Corn

SPARK Symposium Presentations

Avian communities play an essential role in maintaining a healthy ecosystem dynamic by regulating insect populations, assisting in plant pollination, and seed dispersal. These contribute to the overall health of natural ecosystems and mitigate ecological disruptions that would arise in their absence. However, the introduction of non-native invasive plant species can interrupt the balance avian communities bring to ecosystems by reducing availability of food sources, altering habitat structures, increasing competition among individuals, and disrupting ecological relationships through rapid invasion of ecological spaces. Point count observations of birds at Shelby Bottoms Park, Nashville, Tennessee in areas dominated by non-native privet and …


Ashley Hanson '82-Diana Kornett '66, Ashley Hanson, Diana Kornett Jan 2026

Ashley Hanson '82-Diana Kornett '66, Ashley Hanson, Diana Kornett

Alumni Oral History Project

No abstract provided.


Chronic Alcohol Consumption Reprograms Osteoclast Lineage Communications To Promote Osteoclastogenesis, Hami Hemati, Brianna M. Doratt, Ilhem Messaoudi Jan 2026

Chronic Alcohol Consumption Reprograms Osteoclast Lineage Communications To Promote Osteoclastogenesis, Hami Hemati, Brianna M. Doratt, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Chronic alcohol consumption increases the risk of osteoporosis and fracture by disrupting bone remodeling, in part by enhancing osteoclastogenesis. However, the cellular mechanisms underlying this process remain incompletely defined. We analyzed scRNA-seq data from osteoclasts differentiated in vitro from bone marrow mononuclear cells obtained from macaques following 12 months of chronic ethanol or isocaloric control solution consumption. Module scoring, trajectory inference with generalized additive modeling (tradeSeq), and CellChat-based analyses of intercellular communication were applied to uncover ethanol-induced changes in metabolic reprogramming, lineage progression, and signaling network dynamics. Module scoring indicated metabolic reprogramming toward oxidative phosphorylation, with reduced glycolytic, migratory, and …


Can We Close The Rhode Island Women’S Prison?, Nick Horton Jan 2026

Can We Close The Rhode Island Women’S Prison?, Nick Horton

Roger Williams University Law Review

No abstract provided.


Assessing Radio Transmitter Weight Effect And Evaluation Of Northern Bobwhite Chick Survival In Coastal North Carolina, Autumn S. Randall, Theron M. Terhune Ii, Matthew T. Springer, Darin J. Mcneil Jan 2026

Assessing Radio Transmitter Weight Effect And Evaluation Of Northern Bobwhite Chick Survival In Coastal North Carolina, Autumn S. Randall, Theron M. Terhune Ii, Matthew T. Springer, Darin J. Mcneil

Forestry and Natural Resources Faculty Publications

Studies on the earliest life stages are essential to our ecological understanding of avian demography; however, monitoring technologies that allow tracking of small birds are still limited in a variety of ways. One critical limitation, until recently, has been the development of methods for attaching transmitters to young birds with precocial development (e.g., Galliformes, Charadriiformes, etc.). The modified suture technique offers a means to attach transmitters to precocial chicks while accommodating rapid growth characterized by birds with precocial young. Although the sutured transmitter method was developed for northern bobwhite (Colinus virginianus) chicks, there remain key unknowns regarding best …


A Defense Of The Good Samaritan Argument For The Moral Permissibility Of Abortion, Ava Von Pechmann Jan 2026

A Defense Of The Good Samaritan Argument For The Moral Permissibility Of Abortion, Ava Von Pechmann

Pitzer Senior Theses

Judith Jarvis Thomson’s 1971 essay “A Defense of Abortion” is a foundational work in contemporary abortion ethics. It offers a critical analysis of and detailed rebuttal against the argument from a right to life, a prominent anti-abortion argument premised on fetal personhood. Thomson crucially asserts that we may offer a concession to the abortion critic by accepting the premise that a fetus is a person, and still reject the conclusion that abortion is morally impermissible. This is because the right to life does not necessarily entail the right to use another’s body for survival. As such, carrying an unwanted fetus …


“Why Can’T I Do My Homework Without Crying?” Neurodivergence And The Gendered Expectations Of Functioning, Alia Wang Jan 2026

“Why Can’T I Do My Homework Without Crying?” Neurodivergence And The Gendered Expectations Of Functioning, Alia Wang

Pitzer Senior Theses

The purpose of this qualitative interview study was to examine the experiences of neurodivergent women and gender-diverse individuals regarding productivity. Neurodivergence is often understood in biological and medical terms; far less attention has been given to how neurodivergent individuals experience and navigate everyday life. Meanwhile, productivity is often framed as a neutral measure of efficiency, but in practice, it is shaped by social norms that shape particular ways of thinking, working, and behaving.

Particularly for women and gender diverse people, these expectations are compounded not only by cognitive differences but also by gendered norms around behavior. This creates a mismatch …


Evaluating The Effects Of Biochar-Based Slow-Release Nitrogen Fertilizers On Camelina Growth And Development In Greenhouse, Lily Geffre Jan 2026

Evaluating The Effects Of Biochar-Based Slow-Release Nitrogen Fertilizers On Camelina Growth And Development In Greenhouse, Lily Geffre

Schultz-Werth Award Papers

The world’s dependence on conventional fertilizers such as urea allows for significantly increased crop yields but has also greatly contributed to the environmental degradation of agricultural lands and surrounding areas. Biochar-based slow-release fertilizers (SRFs) are an ideal alternative to conventional fertilizers, as they enhance nutrient uptake and retention, improve soil properties, and reduce nutrient leaching and volatilization. This study evaluated the effects of biochar-based SRFs, in comparison with traditional non-SRF fertilizers, on the vegetative growth, physiological growth, and yield of Camelina sativa under greenhouse conditions. Conventional urea yielded the best overall crop results, but a coated biochar-based urea SRF achieved …


Spanish Speakers And Healthcare: Experiences And Perceptions, Ainsley Boucher Jan 2026

Spanish Speakers And Healthcare: Experiences And Perceptions, Ainsley Boucher

Schultz-Werth Award Papers

The Spanish-speaking population in the United States is large and diverse. As such, individual perceptions regarding healthcare access and quality held by native Spanish speakers vary widely. In order to provide insight into local healthcare access, a customized survey was developed and distributed to a workplace in the Brookings area. Responses varied considerably, reflecting a diversity of experiences and perceptions. Determinants such as language barriers, occupational health risks, and various socioeconomic factors may affect healthcare access and quality for native Spanish speakers in the United States.


Chronic Alcohol Consumption Disrupts The Gut Microbial And Metabolic Landscapes, Madison B. Blanton, Ethan G. Napier, Katelyn E. Keen, Ethan V. Stuart, Isaac R. Cinco, Hami Hemati, Ronald C. Bruntz, Landon Wilson, Stephen Barnes, Rupak Khadka, Kathleen A. Grant, Ilhem Messaoudi Jan 2026

Chronic Alcohol Consumption Disrupts The Gut Microbial And Metabolic Landscapes, Madison B. Blanton, Ethan G. Napier, Katelyn E. Keen, Ethan V. Stuart, Isaac R. Cinco, Hami Hemati, Ronald C. Bruntz, Landon Wilson, Stephen Barnes, Rupak Khadka, Kathleen A. Grant, Ilhem Messaoudi

Pharmaceutical Sciences Faculty Publications

Introduction: Alcohol use disorder (AUD) increases incidence of infections, organ damage, and cancers. Aberrant inflammation is likely a driver of these adverse outcomes. Indeed, chronic alcohol consumption (CAC) rewires macrophages/monocytes toward a hyper-inflammatory phenotype. Prior studies showed increased gut permeability and dysbiosis. Translocation of host- and microbial-derived metabolites could trigger the hyper-inflammatory responses generated by macrophages/monocytes. However, the exact changes in these metabolites remain poorly defined due to confounders that complicate clinical studies and the differences between human and rodent gut microbiomes.

Methods: Here, we utilized a non-human primate model of ethanol self-administration to characterize alcohol-induced alterations in gut microbes …


Optimized Nano-Bilosomes Loaded Ocular Insert As A Promising Approach For Boosting Voriconazole Ocular Bioavailability: In-Vitro, Microbiological, And In-Vivo Assessments, Eman Gomaa Jan 2026

Optimized Nano-Bilosomes Loaded Ocular Insert As A Promising Approach For Boosting Voriconazole Ocular Bioavailability: In-Vitro, Microbiological, And In-Vivo Assessments, Eman Gomaa

Nanotechnology Research Centre

The eye possesses numerous physiological barriers that significantly limit the absorption of administered drugs, as only < 5 % get through. These barriers restrict drug bioavailability, often necessitating multiple dosing, which can lead to poor patient compliance. The study goal is to efficiently incorporate Voriconazole (VRC), a wide-spectrum antifungal candidate with a short half-life, into stable, biocompatible, non-irritating bile salt nanoparticles (Bilosomes, BLOs) to enhance its ocular delivery. Bilosomes were prepared by the ethanol injection method. Experimental (33) Box–Behnken Design (BBD) was employed; the amount of bile salt, cholesterol, and span-60 were chosen as independent variables, while entrapment efficiency (EE%), cumulative VRC release (Q8h), particle size (PS), zeta potential (ZP), and polydispersity index (PDI) were the dependent variables. The formula of optimization showed EE% (59.04 ± 1.88 %), cumulative release after 8 h (81.35 ± 0.69 %), PS (222.53 ± 2.04 nm), ZP (−72.53 ± 1.75 mV), and PDI (0.225 ± 0.03). It was chemically characterized using DSC and FTIR. Its spherical arrangement was confirmed using TEM. For ophthalmic application, the optimized formula was included in ocular inserts to extend ocular residence time while minimizing dosing frequency. The optimal VRC-loaded bilosomal ocular insert met the acceptable physicochemical criteria, was sterilized using UV radiation for conducting microbiological assay, in-vivo study, and pharmacokinetic analysis. For safety evaluation, an eye irritation study and histopathological examination were conducted. The results confirmed its effectiveness as evidenced by a significantly larger inhibition zone and 8.39-fold enhancement in VRC bioavailability compared to its suspension. These findings support the potential of bilosomes-loaded ocular insert as an effective ocular delivery of VRC.