Digital Commons Network^™

Synthetic Lethality Between Rb-Loss And E2f3 Inhibition In Small Cell Cancers Targeted By Pyrimidine Synthesis Blockade, Evan R. Abt, Liang Wang, Grigor Varuzhanyan, Jack Freeland, Tian He, Guadalupe M. Peña-Garcia, Lauryn Ruegg, Jami Mclaughlin, Donghui Cheng, Nikolas G. Balanis, Chia-Chun Chen, Yang Xu, Yi Xing, Sanaz Memarzadeh, Caius G. Radu, Thomas G. Graeber, Owen N. Witte

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Small cell carcinoma is a highly lethal cancer variant often found with neuroendocrine (NE) features, as exemplified by small cell lung cancer and small cell NE prostate cancer (SCPC). A genome-wide CRISPR dependency screen using SCPC models generated through human prostate cell transformation identifies a requirement for the transcription factor E2F3. E2F3 dependency is linked to RB inactivation, a near universal occurrence across small cell cancers. The requirement for E2F3 is shared by RB-deficient cells originating from the prostate, lung, and adnexa. In RB-deficient cancer cells, E2F3 inhibition restrains cell cycle progression, proliferation, and tumor growth in vivo. Inhibition of …

Transcriptome Profiling Of Silique Lncrnas Reveals Novel Insights Into Silique Length In Radish (Raphanus Sativus L.), Xiaomin Yu, Yuxin Zhu, Zhoubo Guan, Shisheng Li

Turkish Journal of Botany

Radish is a widely cultivated vegetable with known medicinal value and produces siliques that differ significantly in number and size. The present study compares normal Duanye-13 radish (CK-type) siliques with mutant radish siliques induced by chemical mutagenesis at the young (YS), short (SS), and long (LS) silique stages. Differences in long noncoding ribonucleic acid (lncRNA) expression were investigated using high-throughput RNA sequencing and bioinformatic analyses, revealing 45 324 new lncRNAs in radish siliques. lncRNA expression was higher in YS and LS mutant lines than in CK-type lines, and expression in SS mutants tended to be lower than in CK-type lines. …

Glomerular Basement Membrane Structural Integrity Dictates Trans-Tissue Deposition Of Laminin In The Kidney, Kohei Omachi, Meei-Hua Lin, Pongpratch Puapatanakul, Joshua F Begin, Karen K Mckee, Hironobu Fujiwara, Peter D Yurchenco, Jeffrey H Miner

2020-Current year OA Pubs

Basement membranes (BMs) are specialized extracellular matrices (ECMs) essential for tissue structure and function. In non-vertebrates, ECM components can be produced both locally and by distant tissues. In contrast, mammalian ECM has traditionally been considered to originate predominantly from adjacent or tissue-resident cells. The kidney glomerular basement membrane (GBM), composed of laminin-α5β2γ1 and collagen-α3α4α5(IV), is produced by neighboring cells and functions as a filtration barrier. Alport syndrome, a genetic kidney disease, is characterized by GBM structural defects and ectopic laminin-α2 deposition, but the source of this laminin remains unknown. Here, using CRISPR-Cas9 transgenic models, we demonstrated that ectopic laminin-α2 in …

The George-Anne Inkwell Edition, Georgia Southern University

The Inkwell

No abstract provided.

2,000 Years Of Occupational History And Cultural Change At The Pierce Mounds Complex, An Estuarine Center In Northwest Florida, Hui Xiao

USF Tampa Graduate Theses and Dissertations

Decades of research on Woodland and Mississippian heartlands in the Midwest and Mid-South have greatly enriched our understanding of these ancient societies. More recent studies increasingly emphasize regional variants, recognizing that various environmental settings and local traditions can produce cultural patterns distinct from those of major centers. The Pierce Mounds Complex offers a valuable case for exploring these regional dynamics through an approximately 2,000-year occupational history in an estuarine setting in northwest Florida.

Located at the mouth of Apalachicola River, Pierce is a Woodland and Mississippian center characterized by thirteen burial and platform mounds, extensive shell middens, and associated habitation …

Expanding Access To Latin American Art Song: A Study Of Theodoro Valcárcel's Andean-Influenced Art Songs, Douglas E. Perez

LSU Doctoral Dissertations

EXPANDING ACCESS TO LATIN AMERICAN ART SONG: A STUDY OF THEODORO VALCÁRCEL’S ANDEAN-INFLUENCED ART SONGS advocates for the exploration and performance of underrepresented Latin American composers. Multiple methods of increasing access are discussed, including the creation of a central source for IPA, literal, and poetic translations of Latin American art songs; the study of seldom-performed composers, their output, and influences; and the application of these elements through informed performance. This document centers on the Peruvian composer Theodoro Valcárcel and analyzes the intersection of his indigenous heritage with his Latin American and European musical training as reflected in his compositional style …

Molecular Characterization Of Humanized Apoe Mouse Models Reveals Source And Genotype Dependent Differences, Na Wang, David M Holtzman, Et Al.

2020-Current year OA Pubs

BACKGROUND: Humanized APOE targeted-replacement (TR) mice are essential tools for studying apoE isoform effects in Alzheimer’s disease (AD) and other apoE-related disorders. Despite their widespread use, existing APOE mouse models, generated with different gene targeting strategies, have not been directly compared in terms of apoE isoform expression, lipid profiles, and transcriptomic signatures. Such differences could impact how we interpret APOE genotype-related outcomes, as well as related underlying molecular mechanisms. METHODS: We conducted a comprehensive molecular comparison of humanized APOE mouse models from three sources: Taconic Biosciences (TAC), the Cure Alzheimer’s Fund (CAF), and The Jackson Laboratory (JAX). We assessed apoE …

Tulane Virus Protease As A Structural Surrogate For Inhibitor Screening Of Human Norovirus Proteases, Son Pham, Nikhil Sharma, Banumathi Sankaran, Jalen Nguyen, Mary K Estes, Joseph M Hyser, B V Venkataram Prasad

Faculty, Staff and Students Publications

Human norovirus (HuNoV) is a significant cause of gastroenteritis worldwide, affecting people of all age groups. There are currently no vaccines or drugs available, leaving susceptible populations vulnerable to severe or protracted illness. A HuNoV cultivation system is pivotal for screening norovirus antivirals. While the human intestinal enteroid cultivation system allows robust replication of multiple HuNoV strains, it presents technical and cost barriers. Tulane virus (TV), a surrogate for HuNoV, replicates well in monkey kidney cell lines and is closely related to norovirus in cellular biology. Here, we determined the structures of TV protease (TV-Pro) alone and in complex with …

Heterogeneity And Plasticity Of The Naive Cd4+ T Cell Compartment, Alia Sajani, Evelien Schaafsma, Walburga Croteau, Mohamed Eltanbouly, Elizabeth C Nowak, Chao Cheng, Christopher M Burns, Mary Jo Turk, Randolph J Noelle, J Louise Lines

Faculty, Staff and Students Publications

This study describes the transcriptional heterogeneity of murine and human naive CD4+ T cells as comprising multiple discrete clusters that impact CD4+ T cell fate and trajectories. Naive CD4+ T cells experiencing inflammatory environments exhibit an altered transcriptional state that biases their differentiation trajectories.

Genome-Wide Crispr Screen Identifies A Cytokine-Enhancer Circuit Driving Hif-2Α Activation In Renal Cancer, Jun Fang, Jeremy M Simon, Tao Wang, Yunpeng Gao, Xianju Bi, Lianxin Hu, Chengheng Liao, Cheng Zhang, Yayoi Adachi, Jin Zhou, Hongyi Liu, Qian Liang, James A Nathan, Ram Mani, James Brugarolas, Qing Zhang

Faculty, Staff and Student Publications

Resistance to HIF-2α inhibitors such as Belzutifan underscores the need to better understand how HIF-2α is transcriptionally regulated in clear cell renal cell carcinoma (ccRCC). Here, we uncover a cytokine-driven enhancer mechanism that sustains HIF-2α expression through the JAK1-STAT3 signaling pathway. Using a genome-wide CRISPR screen in VHL-deficient ccRCC cells, we identified SOCS3 as a key negative regulator of HIF-2α. Mechanistically, loss of SOCS3 activates JAK1-STAT3 signaling, leading to the recruitment of STAT3 to distal enhancers upstream of EPAS1 that physically loop to its promoter to drive HIF-2α transcription. This cytokine-enhancer circuit was recapitulated in ccRCC patient samples and functionally …

Eoq Model For Deteriorating Items With Exponential Demand Rate, Price Dependence And Shortages Under Trapezoidal And Neutrosophic Fuzzy Techniques, Partha Ray, Agniva Dey, Somen Seal

Neutrosophic Sets and Systems

No abstract provided.

Class Of Analytic Function Defined By Neutrosophic Poisson Distribution, Olushola Adeyemo, Sayo Abidemi Gbangbala, Adeniyi Abimbola Ayeni, Folorunso Isola Akinwale, Abel Onaolapo Aremu

Neutrosophic Sets and Systems

No abstract provided.

Lipocalin 2 Orchestrates Resistance To Ferroptosis Via Axl, Sabrina Z Wang, J Payton Timken, Ellen S Hong, Sehaj Kaur, Eli Newby, Kristen E Kay, Erin E Mulkearns-Hubert, Daniel J Silver, Juyeun Lee, Joshua B Rubin, James R Connor, Loic P Deleyrolle, Deanna Tiek, Andrew Dhawan, Justin D Lathia

2020-Current year OA Pubs

Glioblastoma (GBM) remains a lethal tumor, largely due to robust mechanisms that prevent effective induction of cell death. Ferroptosis, a form of iron-dependent cell death, is a promising vulnerability in GBM. Here, we demonstrate that lipocalin-2 (LCN2) suppresses ferroptosis in GBM cells via the receptor tyrosine kinase AXL. LCN2 was elevated in GBM cells compared to lower-grade tumor and non-transformed cells, and Lcn2 knockdown impaired GBM cell fitness and growth in vitro and in vivo. Mechanistically, Lcn2 knockdown triggered ferroptosis, which was specifically rescued with ferroptosis inhibitors but not apoptosis or necroptosis inhibitors. Lcn2 knockdown reduced AXL phosphorylation, which was …

Efficacy And Safety Of Stem Cell-Based Interventions For Burn Wound Healing: A Systematic Review And Meta-Analysis Of Pre-Clinical And Early Clinical Evidence, Prayuth Poowaruttanawiwit, Patsanan Kulla, Naveeya Pakpoomkamonlert

The Thai Journal of Pharmaceutical Sciences

Background: Burn injuries remain a major therapeutic challenge, with conventional treatments often failing to achieve complete functional skin regeneration. Stem cell-based interventions, including mesenchymal stem cells (MSCs) and exosome derivatives, have demonstrated regenerative and immunomodulatory potential, yet their translation to clinical practice remains limited.
Objectives: To systematically evaluate the efficacy and safety of stem cell-based therapies for burn wound healing across pre-clinical and clinical studies.
Materials and Methods: Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines, four databases (PubMed, Embase, Scopus, and Cochrane) were searched through April 2025. Eligible studies included randomized controlled trials and controlled pre-clinical …

Identification Accuracy Of Cost-Effective Visible (Vis) To Near-Infrared (Nir) Sensor As7265x On Plastic Waste In The Philippines, Ma. Tricia Jasmin M. Ocho, Chloe Lizanne R. Bravo, Jennika Love R. Loyola, Kierra Jamielle Velandria Taroma, Leianne Nina Arabelle E. Lampayan

DLSU Senior High School Research Congress Conference Proceedings

In the Philippines, plastic waste persists as a significant issue caused by the widespread use of single-use plastics and the lack of recycling systems. The lack of proper systems made waste segregation and identification inefficient and disorganized. Past studies explain the use of VIS/NIR in plastic segregation and the potential of spectral frequencies. However, there is limited investigation into the consistency of the AS7265x sensor to address reliability in real-world conditions, where contaminants, variable lighting, and material degradation significantly impact performance. Thus, the study evaluates the effectiveness of the AS7265x triad spectroscopy sensor, incorporating machine learning algorithms. An experimental setup …

Development Of An Mhealth Application To Be Used In Assisting Caregivers, Samantha Lianne Lim Cheong, Sofia Margarita S. Cristobal, Precious Channe Esther S. Dioquino, Matteo G. Guanzon, Kyle Ian Jeffrey Lim Lim

DLSU Senior High School Research Congress Conference Proceedings

Assistive technology refers to any product, device, or system that helps individuals with their care and aids with tasks that have become difficult due to physical, cognitive, or sensory limitations. Within the scope of assistive technology are mobile health (mHealth) applications, which utilize mobile devices to provide healthcare services and information. Due to the mHealth applications' implementations on mobile devices, their adaptability is universal and accessible. This research aims to develop a mHealth application that is primarily accessible and designed specifically for caregivers by using their recommendations as the main references alongside literature reviews. It explores the features of mHealth …

Iah-Net (Informative Air Quality And Heat Index Network): Drone-Assisted System For Real-Time Air Quality And Heat Index Monitoring, Brent Breo B. Magbanua

DLSU Senior High School Research Congress Conference Proceedings

Air pollution and rising temperatures threaten public health and safety, necessitating advanced monitoring solutions. This study developed a drone-assisted system for autonomous monitoring of air quality and heat index, ensuring real-time alerts even in the absence of human presence. By integrating the K-Nearest Neighbors (K-NN) machine learning algorithm, the system provides an innovative and cost-effective approach to identifying environmental risks and raising awareness. The system consists of a Drone system, ESP32, MQ-135 gas sensor, and DHT11 temperature and humidity sensor for data acquisition. Software development includes implementing the K-NN algorithm and calibrating sensors to enhance classification accuracy. The system categorizes …

Medicines Security And Bioprospecting: A Future Agenda For Pharmaceutical Research, Geoffrey A. Cordell

The Thai Journal of Pharmaceutical Sciences

Humanity is at an extraordinary moment in history, where our interwoven future in Gaia depends on showing reverence for the modulation of our resource limits after significant losses of biodiversity and encroaching environmental change, including disappearing glaciers, rising sea levels, and changing climates. Engaging with the interconnectedness of all life on Earth and integrating sustainability and optimization of the valuable, renewable resources for future generations are essential beginning actions. Fostering economic and resource sustainability deepens the focus for contemporary research priorities toward assuring access to biological agents needed by the consumer/patient. Based on the outcomes of the COP28/29/30 and COP15/16 …

Plastilapia: Potential Of Tilapia (Oreochromis Niloticus) Scales For Creating Bioplastics, Rain Andrea Q. Matilde, Kathlene Ann R. Aspiras-Lorenzo

DLSU Senior High School Research Congress Conference Proceedings

Plastic waste is a global problem affecting General Santos City (GenSan). Though brown paper bags are alternatives, they tear easily and may cause deforestation. South Cotabato, including GenSan, produces large amounts of tilapia. Their discarded scales contain collagen that can be used to create sustainable biopolymers. This study explores the potential of tilapia scales for creating bioplastics, focusing on tensile strength and biodegradability as eco-friendly alternatives to plastics. For this study, one kilogram of raw tilapia fish scales was used. The scales were cleaned, oven-dried at 60°C for three hours, ground, and soaked in a 0.1N sodium hydroxide solution for …

Pinenergy: A Sustainable Bio-Piezoelectric Generator From Queen Pineapple Crown Leaves (Ananas Comosus [Linn.] Merr.) For Energy Generation, Harvesting, And Conversion, Angelyzha Julienne V. Carpio, Rhionna Marie Marcelo Mayor, Mark Cyril C. Brazil, Phebe P. Mendoza

DLSU Senior High School Research Congress Conference Proceedings

Energy is one of the necessities in life. The Philippines has abundant energy resources; however, as the energy demand increases, energy reserves are diminishing faster than they can be replenished, and alternatives remain underutilized due to cost and ethical challenges, resulting in a shortage in energy production. To combat this, bio-piezoelectricity, which transforms mechanical forces into electrical energy through an organic material, offers a secure substitute for current energy resources. This study aims to determine the efficacy of queen pineapple crown leaves (QPCL) as a bio-piezoelectric generator in energy generation, harvesting, and conversion efficiency. The leaves served as the primary …

Optimizing Sea Grapes (Caulerpa Lentillifera) Extract Concentration For Maximum Larval Mortality, Bench Russel D. Caol-Olan, Nicole Shane D. Sabaldan, Jullian Jake D. Labastida, Steffany C. Illagan, Herlynne C. Mallari, Raven Pauline A. Ebuen, Jairra Lysandra M. Atun, Deness Patricia Lauress P. Turla, Dana Kriesha E. Prudente, Christian Dale Luis F. Valenzuela, June Ray O. Filler

DLSU Senior High School Research Congress Conference Proceedings

This research investigates the larvicidal potential of Caulerpa lentillifera, commonly known as sea grapes. Specifically, it aims to determine whether 0%, 10%, 20%, or 30% concentrations of C. lentillifera extract can significantly increase mosquito larval mortality after four hours of exposure. The experiment followed standardized guidelines established by the World Health Organization (WHO), and larval mortality was calculated using Abbott’s formula. This research investigates the larvicidal potential of C. lentillifera, commonly known as lato sea grapes. Specifically, it aims to determine whether 0%, 10%, 20%, or 30% concentrations of C. lentillifera extract can significantly increase mosquito larval mortality after four …

Teaching The Whole Child Through Project Based Learning, Loren Ryan

Teacher Education Journal of South Carolina

This paper describes in detail the current teaching practices integrating Project Based Learning with explicit examples of projects administered at the gateway grades of 4th, 8th, and 12th grade, respectively. Incorporating Project Based Learning at a local independent school in Columbia, South Carolina, offers students the opportunity to demonstrate their cumulative knowledge at each gateway grade while allowing students to integrate the arts into their exhibition of mastery.

The Pedagogical Implications Of School Choice Initiatives In South Carolina, A. Scott Henderson

Teacher Education Journal of South Carolina

School choice theory (SCT) is an important component of contemporary discussions about educational reform. For the past several years, South Carolina lawmakers have attempted to enact legislation based on SCT principles. These attempts, albeit unsuccessful, illustrate recurring concerns and suggest a need for teacher preparation programs to address more fully the pedagogical implications of school choice initiatives.

Full Issue: Volume 2, 2009, Christopher Burkett

Teacher Education Journal of South Carolina

This file contains the full issue of TEJSC, including front matter, table of contents, editorial notes, and articles.

Old Dogs, Jesse A. Parkman

Landshark Literary Review

No abstract provided.

Defying The Conventional: Musical Performance, Embodied Cognition, And The Reconfiguration Of Institutional Discourse, Dillon Parmer

Music & Musical Performance: An International Journal

This essay confronts the dialectic between theory and practice through a comparison of an idea basic to how we understand music (the notion that pitch moves up and down in vertical space) with how pitch is thought of in actual music-making. The comparison leads to both a new conceptual model for thinking about pitch, what might be called Pitch Horizontality, as well as a proposal for how to bring institutional discourse about music into sync, not with how scholars in other disciplines think about their subject matter, but with the thinking that goes on in contexts of real-world artistic production.

Savior Complex, Stereotyping, Sensationalism Of The Filipino Poor: Critical Media Analysis Of Raffy Tulfo In Action’S Charity Episodes, Charisse Anne C. Bautista, Jamella Panes Jamolangue, Yellowbelle Duaqui

DLSU Senior High School Research Congress Conference Proceedings

In the Philippines, one of the significant issues in society is the continuous growth of poverty and inequality. In the field of media, poverty porn is a term used to describe any content that exploits the images and narratives of impoverished individuals to gain sympathy, notoriety, and funds for a particular cause. However, there is a lack of research on poverty porn in the social media context, especially in the Philippine landscape. Thus, this study focuses on the portrayal of poverty in social media, specifically in the charity content of Raffy Tulfo in Action YouTube channel. Inductive content analysis was …

Transcranial Alternating Current Stimulation As An Adjuvant For Nonfluent Aphasia: A Proof-Of-Concept Study, Lynsey M. Keator, Lisa Johnson, Roger Newman-Norlund, Kyler Spell, Samaneh Nemati, Leigh Ann Spell, Dirk B. Den Ouden, Christopher Rorden, Julius Fridriksson

Department of Speech-Language Pathology Faculty Papers

Effective rehabilitation tools are essential for improving language outcomes in chronic aphasia. Speech entrainment is a behavioral treatment that has shown promise in enhancing speech output in nonfluent aphasia, potentially by acting as an external mechanism to synchronize anterior and posterior language regions in the left hemisphere. Transcranial alternating current stimulation has been hypothesized to enhance functional connectivity between brain regions by amplifying endogenous oscillations. This proof-of-concept study explored whether high-definition tACS (HD-tACS) could improve speech fluency in nonfluent aphasia when paired with speech entrainment. In a double-blind, pseudorandomized study, 1 mA of HD-tACS at 7 Hz was applied to …

Bacteriophage Therapy For Antibiotic-Resistant Infections, Shiva Pariyar

Best Integrated Writing

Pariyar examines bacteriophage therapy as a promising alternative to antibiotics for treating multidrug-resistant infections, highlighting its targeted mechanism, ability to disrupt biofilms, and synergy with antibiotics. While challenges such as regulation, immune response, and limited clinical trials remain, the study argues that advances in biotechnology and global collaboration position phage therapy as a future cornerstone in combating antimicrobial resistance.

Assessment Of Setup Errors In Breast And Uterus Radiotherapy Using Epid, Zainab Abdul-Qader, Hiba Al-Hameed

Baghdad Science Journal

The difference between the true treatment image represented by the portal image and a picture digitally reconstructed radiograph (DRR) coming from the physics planning as the reference image helps us measure displacement. The treatment area is dependent on the edges of the setting error. The essential step is to estimate the setting error during treatment. Dosage allocation at PTV (planning treatment volume) follows on set-up edges. Set up errors for the radiation oncology collective to minimize the therapy error. Position errors explain the variance between the first position the patient was scanned at the Computer Tomography (CT) simulation and the …