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2026

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Structural Evidence For Innervation Of A Single Epitheliomuscular Cell By Multiple Sensory Neurons In The Sea Anemone Nematostella Vectensis, Aparajita Das May 2026

Structural Evidence For Innervation Of A Single Epitheliomuscular Cell By Multiple Sensory Neurons In The Sea Anemone Nematostella Vectensis, Aparajita Das

Graduate Theses and Dissertations

Abstract Here, I report new ultrastructural evidence for multineuronal innervation (many-one) of a single epithelia-muscular cell in sea-anemone cnidarian Nematostella vectensis. Using Serial Block-Face Scanning Electron Microscopy (SBF-SEM) and ImageJ, I found that EMCs are located at the base of ectoderm, with an elongated cell body that includes basal processes, a bilobed nucleus, and myofibrils aligned parallel to the basal surface along with their structural quantification. Notably, I found multiple mechanosensory neurons form synapses with a single EMC. These findings enhance our understanding of cnidarian neuromuscular organization and reveal that coordination between sensory and contractile systems in N. vectensis is …

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Will The Demons Follow (And Other Stories), Eberechi Onyekachi Mcbride May 2026

Will The Demons Follow (And Other Stories), Eberechi Onyekachi Mcbride

Graduate Theses and Dissertations

Will The Demons Follow (and Other Stories) is a collection of interconnected short stories that examines memory, trauma, migration, and womanhood across Nigerian and diasporic spaces. Set in both Nigeria and the United States, the stories move between villages, cities, and immigrant communities, presenting characters whose lives are shaped by cultural expectations, spiritual beliefs, family authority, and personal longing. Through multiple narrative voices, the collection considers how the past continues to shape the present. Characters struggle with family conflict, religious pressure, sexual violence, and the psychological burdens carried across borders. In the title story, “Will The Demons Follow?”, the narrator …

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Exploring A 'Fishy' Solution: Uncovering Human Norovirus Using A Zebrafish Model, Sahaana Chandran May 2026

Exploring A 'Fishy' Solution: Uncovering Human Norovirus Using A Zebrafish Model, Sahaana Chandran

Graduate Theses and Dissertations

Human norovirus (HuNoV) is the leading cause of acute gastroenteritis worldwide; however, the lack of robust laboratory culture systems and reliable animal models has limited progress in understanding viral replication and developing effective intervention strategies. While alternative models such as the zebrafish system have been explored, gaps remain in evaluating their ability to support replication across diverse HuNoV strains and in identifying factors that influence infectivity. This dissertation provides a comprehensive investigation into the use of the zebrafish model for studying viral replication, host interactions, and potential inhibition strategies. Initial studies focused on optimizing viral delivery methods using Tulane virus …

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Navigating Mixedness As A Multiracial Teacher, Casey S. Kraichoke May 2026

Navigating Mixedness As A Multiracial Teacher, Casey S. Kraichoke

Graduate Theses and Dissertations

This qualitative hermeneutic phenomenological study utilized Critical Multiracial Theory (MultiCrit) to construct a counternarrative that amplifies the voices of Multiracial teachers, focusing on the development of personal and teacher identity. Specifically, the study critically examines the trajectory of Multiethnic-Racial (ME-R) identity transformation following experiences teaching English as a Foreign Language (EFL) in participants’ heritage Asian countries. Data were collected through one-time semi-structured virtual interviews with fifteen purposefully selected Asian-White Multiracial EFL teachers who had completed a one-year Fulbright English Teaching Assistantship (ETA) within the past decade. Each participant also provided a written artifact to support the analysis. Participants reflected on …

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The Line In The Sand: Visual Narratives Of Religious Trauma And Leaving Evangelicalism, Elizabeth Humphrey May 2026

The Line In The Sand: Visual Narratives Of Religious Trauma And Leaving Evangelicalism, Elizabeth Humphrey

Graduate Theses and Dissertations

Widespread abuse within religious institutions has challenged the assumption that religion inherently supports mental health, while rising rates of religious disaffiliation often cite trauma as a key factor. Individuals leaving harmful religious environments report a range of outcomes—from increased autonomy to emotional distress—yet research on post-disaffiliation experiences is limited. As the religious landscape of the U.S. shows decrease in religious affiliation, and stories of abuse and trauma circulate, the experience of leaving a religious community because of harm is a needed area for further inquiry. Using visual narrative inquiry, this qualitative research study explores the lived experiences of those who …

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Bats And Bacteria: Infection Dynamics At The Human-Wildlife Interface, Isabella Katherine Deanglis May 2026

Bats And Bacteria: Infection Dynamics At The Human-Wildlife Interface, Isabella Katherine Deanglis

Graduate Theses and Dissertations

As the second most diverse mammalian order, bats host an array of pathogens, some of which are known to infect humans. Synanthropic bats share spaces with humans, resulting in bat-borne pathogen exposure. While most bat-borne disease research has focused on viral pathogens, bats host numerous bacterial pathogens with zoonotic potential. By integrating a global meta-analysis with field studies in Sub-Saharan Africa, I characterize the structural attributes that facilitate shared interfaces, the bacterial pathogens present in synanthropic bats of rural Kenya, and the physiological and environmental dynamics influencing odds of infection in these synanthropic bats. Finally, I evaluate the clinical relevance …

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A Survey Of The Collembola Of The Buffalo National River Park, Emma Renee Critchfield May 2026

A Survey Of The Collembola Of The Buffalo National River Park, Emma Renee Critchfield

Graduate Theses and Dissertations

Collembola are a neglected and understudied class with few recent U.S surveys. The aim of this thesis was to survey the Buffalo National River for Collembola and create a catalogue of all known species in the state of Arkansas, USA. Leaf litter, mosses, rotting wood, fungi and malaise trap samples were taken from 13 locations within the Buffalo National River park. In total, 14 families, 34 genera and 59 species were identified from 22,028 collected specimens. Of those, 31 species represent new state records. This survey also validates malaise traps as useful tools for Collembola sampling. The malaise trap samples, …

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Evaluating The Role Of Hosa–Future Health Professionals In Addressing The Healthcare Workforce Shortage, Amy Warner May 2026

Evaluating The Role Of Hosa–Future Health Professionals In Addressing The Healthcare Workforce Shortage, Amy Warner

Graduate Theses and Dissertations

The United States continues to face a significant healthcare workforce shortage, particularly in rural and underserved communities, with implications that extend globally. As a practitioner in health science education, this study was driven by a problem of practice: understanding how HOSA–Future Health Professionals contributes to developing the future healthcare workforce. While the need for effective workforce pipeline strategies is widespread, this study is centered specifically on Oklahoma HOSA as a model of practice within one state. HOSA is designed as an intracurricular extension of health science programs, providing students with opportunities to apply classroom learning through leadership development, competitive events, …

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Religion, Crime, And The Mediating Effects Of Social Control And Self-Control, Parker Bowman May 2026

Religion, Crime, And The Mediating Effects Of Social Control And Self-Control, Parker Bowman

Graduate Theses and Dissertations

Past research on the relationship between religion and crime has found the two variables have a significant negative correlation. Currently, there are two main mechanisms used to explain this relationship: social control and self-control. Social control theorists argue that participation in religious organizations decreases criminal behaviors by increasing social bonds. Self-control theorists argue that religious norms and values decrease criminal behavior by increasing an individual's self-control. While both mechanisms have been well supported by research, there remains the question of which is the stronger mediator of the religion-crime relationship. This study seeks to answer this question by comparing the effects …

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Simultaneous Carrier, Cargo, And Functional Tracking Of Lipid Nanoparticles, Hannia Vanessa Balcorta Muñoz May 2026

Simultaneous Carrier, Cargo, And Functional Tracking Of Lipid Nanoparticles, Hannia Vanessa Balcorta Muñoz

Open Access Theses & Dissertations

Lipid nanoparticles (LNPs) have become effective delivery vehicles in nanomedicine, particularly for nucleic acid-based treatments. However, a major limitation remains in the field regarding the simultaneous monitoring of the nanoparticle carrier, its encapsulated payload, and the resulting functional protein production in both in vitro and in vivo systems. Furthermore, traditional lipophilic dyes employed for labeling LNPs frequently exhibit incompatibility with tissue-clearing methods, such as CLARITY, due to signal loss following lipid removal. This research developed and evaluated a new fluorescent labeling method using CM-DiI, a dye that reacts with thiols, for multimodal tracking of LNPs. CM-DiI was included in the …

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Blood, Steel, And Stone, Jennifer Camplair Hassell May 2026

Blood, Steel, And Stone, Jennifer Camplair Hassell

Open Access Theses & Dissertations

This thesis, Blood, Stone, and Steel, explores the intergenerational impact of trauma, memory, and history through a unique blend of poetry, photography, and personal family narratives. Drawing on archival materials, family photographs, and the tradition of documentary poetry, the work investigates how the legacy of war and immigration shapes identity across generations. Inspired by Marianne Hirsch's concept of postmemory, the author examines inherited memories and their affective transmission, focusing on the experiences of her great-grandfather - a German immigrant and WWI veteran - and the subsequent effects on her family. The narrative is structured in three parts: BLOOD, reflecting loss …

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Spike-Sorting Algorithm For Neuropixel Probes, Luis David Davila May 2026

Spike-Sorting Algorithm For Neuropixel Probes, Luis David Davila

Open Access Theses & Dissertations

Modern extracellular neural probes like Neuropixels, allow for high-density extracellular neural probes to record terabytes of electrophysiological brain data. Spike sorting is the process of isolating individual neurons on this data through various clustering and filtering techniques. Modern spike sorting algorithms are held back by their need for human intervention to separate high-quality from low quality clusters, due to the volume of data that can be collected a completely automated approach is required. To address this requirement, we have developed a framework to preprocess, cluster, grade, and separate high/low quality clustering results using custom models trained on the grades. To …

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Dungeons And Dragons As Game-Based Learning, Moses Allen May 2026

Dungeons And Dragons As Game-Based Learning, Moses Allen

Language, Literacy, and Sociocultural Studies ETDs

This phenomenological qualitative study explored the use of the Dungeons and Dragons (D&D) Role-Playing Game as a Game-Based Learning (GBL) model, measuring middle school student perceptions of engagement, motivation, and skill development through interactive storytelling. The study used a Practitioner Research theoretical framework and Thematic Analysis to identify emergent themes from the collected data. The study included gameplay of D&D after school with a small group of participants grades 6-8. Results indicated that participants reported consistently high levels of motivation and engagement. Participants perceived that D&D gameplay promoted the development of 21st Century Skills and middle school curricula skills, specifically …

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The Phylogenetic And Biogeographic History Of Cranes (Gruidae), Phillip Conrad Williams May 2026

The Phylogenetic And Biogeographic History Of Cranes (Gruidae), Phillip Conrad Williams

Dissertations

Cranes (Gruidae) are a globally distributed clade of large, wetland-associated birds. Prior phylogenetic analyses have shown there to be two major subfamilies, Balearicinae and Gruinae, and five consistently recovered clades within Gruinae. However, the relationships among those five clades has remained contentious. In addition, little has been done to reconstruct the biogeographic history of cranes. To that end, I first inferred phylogeny using a large, nuclear dataset sampled from all 15 extant crane species and two outgroup species. The phylogeny mostly agreed with previous analysis using whole mitochondrial genomes but differed in that the Anthropoides species group formed a sister …

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Intelligent Surveillance For Weapon Threat Detection: Transformer-Based Detection And Segmentation Using Thermal And Rgb Imagery, Akhila Kambhatla May 2026

Intelligent Surveillance For Weapon Threat Detection: Transformer-Based Detection And Segmentation Using Thermal And Rgb Imagery, Akhila Kambhatla

Dissertations

Proactive public-safety surveillance requires deep learning systems that can detect and segment weapons reliably under real-world conditions. This research develops and evaluates a progressive, multi-phase pipeline that moves (i) from RGB object detection to pixel-level semantic segmentation and (ii) from visible-spectrum cameras to thermal imagery to improve robustness under low-light, clutter, and occlusion. Early phases establish strong detection baselines using one-stage and two-stage detectors (YOLOv5 and Faster R-CNN) for multi-class weapon localization, followed by practical optimization through pruning and ensembling to reduce false positives and computational overhead while maintaining real-time feasibility. Building on the limitations of bounding-box localization, the methodology …

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Robots And Ageing Population – Rehabilitation And Social Robots, Yuzhou Qian, Keng Siau May 2026

Robots And Ageing Population – Rehabilitation And Social Robots, Yuzhou Qian, Keng Siau

Research Collection School Of Computing and Information Systems

Population ageing is placing increasing pressure on healthcare and caregiving worldwide. Governments in different countries are looking into the use of advanced information technologies such as artificial intelligence and robotics to help in caring for ageing population. Older adults often need both physical assistance and psychological support, and robots have emerged as a promising technological approach to supplement eldercare. This paper provides an overview of the current state of robots in eldercare. It reviews the major definitions, categories, and features of robots used in healthcare and eldercare. The paper also examines the potential benefits of robotic technologies, such as reducing …

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Diacylglycerol Kinase Eta As A Novel Target In Nras Mutant Melanoma, Haley P. Wilson, Casey D. Stefanski, Signe Caksa, Glenn L. Mersky, Scott D. Varney, Jelan I. Haj, Dan A. Erkes, Timothy J. Purwin, Vivian Chua, Andrew E. Aplin May 2026

Diacylglycerol Kinase Eta As A Novel Target In Nras Mutant Melanoma, Haley P. Wilson, Casey D. Stefanski, Signe Caksa, Glenn L. Mersky, Scott D. Varney, Jelan I. Haj, Dan A. Erkes, Timothy J. Purwin, Vivian Chua, Andrew E. Aplin

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

NRAS mutations occur in 10%-30% of cutaneous melanomas and are associated with high tumor mutational burden. Mutant NRAS signaling drives aberrant cell growth and proliferation, in part, through activation of the RAF-MEK-ERK1/2 kinase pathway; however, targeted therapies to this pathway have limited effectiveness in patients with NRAS mutant melanoma. The role of other targetable signaling pathways in NRAS mutant melanoma is poorly characterized. Here, we demonstrated that one isoform of diacylglycerol kinase, diacylglycerol kinase eta (DGKη), a lipid signaling regulator, was highly expressed in NRAS mutant melanoma patient samples. Knockdown of DGKH in NRAS mutant melanoma cell lines resulted in …

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Something Doesn’T Add Up: Hhs Complicated By Xylazine Withdrawal, Rahul Nimmagadda Do, Daniel Logatto Do, Joshua Kepfinger Do, Breanna S. Goldner Do May 2026

Something Doesn’T Add Up: Hhs Complicated By Xylazine Withdrawal, Rahul Nimmagadda Do, Daniel Logatto Do, Joshua Kepfinger Do, Breanna S. Goldner Do

Department of Medicine

No abstract provided.

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Twenty-Four-Hour Intermittent Fasting Extends Lifespan At The Expense Of Fecundity In Daphnia, Meridith L. Smith May 2026

Twenty-Four-Hour Intermittent Fasting Extends Lifespan At The Expense Of Fecundity In Daphnia, Meridith L. Smith

Undergraduate Honors Theses

Dietary restriction (DR) is a well-established intervention for extending lifespan, yet the specific efficacy of intermittent fasting (IF) and its associated trade-offs remain under-explored in many Daphnia magna. This study investigated the effects of three IF intervals—2h, 24h, and 48h—on the longevity and fecundity of two clones of Daphnia. We hypothesized that longer fasting intervals would significantly extend lifespan at the cost of reproductive output, consistent with the disposable soma theory of resource allocation.
Our results demonstrate that 24h IF significantly increased longevity relative to ad libitum controls, even outperforming caloric restriction (CR) by reducing late-life mortality. In …

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A Planetary Health Approach To Address Food Insecurities In New Mexico, Mariah Kennell May 2026

A Planetary Health Approach To Address Food Insecurities In New Mexico, Mariah Kennell

Doctor of Nursing Practice Scholarly Projects

Food insecurity remains a persistent public health concern throughout New Mexico, impacting both rural and urban populations and contributing to significant health disparities. Limited access to affordable, locally sourced fresh foods exacerbates chronic disease burden and undermines community health. The purpose of this Doctor of Nursing Practice project was to conduct a comprehensive policy analysis to identify feasible state-level strategies to improve food access through strengthening local food systems. Using the Centers for Disease Control and Prevention POLARIS policy analysis framework, a structured evaluation of existing and potential state and community-level policies was conducted. The analysis incorporated stakeholder interviews with …

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Unravelling Taxonomic Complexity In Elusive Cetaceans: Mitogenome Insights Into Evolutionary History And Cryptic Diversity Of Bryde's Whales, Dominique Paynee, Paulette Bloomer, Gwenith Penry, Simon Elwen, Ada Natoli, Vaisakh Dhanalakshmy, Thyago Cardoso, Els Vermeulen May 2026

Unravelling Taxonomic Complexity In Elusive Cetaceans: Mitogenome Insights Into Evolutionary History And Cryptic Diversity Of Bryde's Whales, Dominique Paynee, Paulette Bloomer, Gwenith Penry, Simon Elwen, Ada Natoli, Vaisakh Dhanalakshmy, Thyago Cardoso, Els Vermeulen

All Works

Cetaceans, despite their ability to cross wide distances, enabling mostly high levels of gene flow, can develop unique genetic lineages driven by variations in life-history traits, diverse migratory routes and local adaptation, complicating taxonomy and delineation of conservation units. Comprehensive genetic data are essential to overcome the challenges of obtaining suitable sample sizes from wild cetaceans while untangling cryptic species diversity. The Bryde's whale (Balaenoptera edeni) has a complicated discovery and taxonomic history that hinders accurate conservation assessments. In southern Africa, two populations are recognised: the inshore (SAi) and the offshore southeast Atlantic population (SEA). This study examines their phylogenetic …

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Restorying The Reader: Adolescent Narratives Of Identity, Context, And Motivation, Jennifer R. Gardner May 2026

Restorying The Reader: Adolescent Narratives Of Identity, Context, And Motivation, Jennifer R. Gardner

Dissertations

This present study examined how adolescents narrate their reading experiences and what those narratives reveal about reading identity, motivation, and engagement. This study responds to a persistent problem in literacy education: adolescent reading is often framed through disengagement or reduced to isolated constructs, while students’ lived reading experiences across school, home, digital, linguistic, and social contexts remain underexamined. Guided by Self-Determination Theory, Intersectionality, and Reader Response Theory, this study centers on adolescent voices and treats these participants’ stories as the primary avenue of understanding how they experience and interpret their reading lives and selves. Data were collected through narrative interviews, …

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Qualitative Phenomenography: Sustaining Motivations Of Black Women Principals In Pk-5 Title I Schools, Kahliah C. Rachel May 2026

Qualitative Phenomenography: Sustaining Motivations Of Black Women Principals In Pk-5 Title I Schools, Kahliah C. Rachel

Dissertations

Black women principals remain underrepresented in educational leadership, yet they are disproportionately assigned to PK-5 Title I schools, where complex academic, social, and political demands converge. Limited research examines what sustains these leaders over time, particularly in roles marked by intersecting oppressions of race and gender. Grounded in Black feminist thought and intersectionality, this qualitative phenomenographic study explored the sustaining motivations of six Black women who currently served or had served as principals in a PK-5 Title I school in Georgia. The purpose of this study was to describe the different ways these principals understood and experienced the internal and …

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Traumatic Stress Recruits An Excitatory Orbitofrontal-Amygdala Pathway To Drive Maladaptive Aggression, Mikaela Aholt May 2026

Traumatic Stress Recruits An Excitatory Orbitofrontal-Amygdala Pathway To Drive Maladaptive Aggression, Mikaela Aholt

Theses

Traumatic stress is a reliable predictor of heightened aggression, yet the mechanisms linking stress exposure to aggression remain poorly understood. The Nordman lab previously found that traumatic stress activates the posterior ventral segment of the medial amygdala (MeApv) to drive persistent increases in aggressive behavior. However, why traumatic stress would engage an aggression-promoting pathway is unclear. To address this, the inputs to the MeApv were mapped and a population of excitatory, but not inhibitory, neurons in layer 5 of the medial orbitofrontal cortex (mOFC) were identified. The OFC is classically viewed as an inhibitory brake on amygdala-driven impulses, yet emerging …

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Evaluation Of Otolith Microchemistry For Identifying Walleye Stocked As Fry Or Fingerlings, Joshua Fluur May 2026

Evaluation Of Otolith Microchemistry For Identifying Walleye Stocked As Fry Or Fingerlings, Joshua Fluur

Theses

Walleye (Sander vitreus) is a popular sport fish whose populations are often supported by maintenance or supplemental stocking, with most fish stocked as fry, fingerlings, or advanced fingerlings. Evaluation of stocking efficacy is important to inform decisions regarding fish stocking rates and sizes and allocating hatchery fish to locations where maintenance or supplemental stocking is most needed. Several types of artificial tags or marks have been proven useful in the identification of stocked fish, but they often have various drawbacks. Natural chemical markers in otoliths offer several advantages compared to conventional tags or marks and can be used to identify …

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The Making Of Shi'a Resistance In Afghanistan: Historical Roots, Communist Rule, And Soviet Occupation, 1978–1989, Sayed Sajjad Hojet May 2026

The Making Of Shi'a Resistance In Afghanistan: Historical Roots, Communist Rule, And Soviet Occupation, 1978–1989, Sayed Sajjad Hojet

Theses

This thesis examines Shi'a political mobilization in Afghanistan from the 1978 Saur Revolution through the Soviet occupation, ending in 1989. It argues that Shi'a resistance was not simply a religious reaction to Marxist rule but a complex movement shaped by long-term marginalization, influenced by rival political ideas, and connected to cross-border networks in Iran. It traces how persecution under Amir Abdurrahman Khan created a system of political exclusion that shaped Shi'a collective identity before the communist takeover, and how ideologies such Marxism and Political Islam further politicized Shi'a youth in the mid-twentieth century. The PDPA's coercive policies after 1978 helped …

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Relationships Between Therapists And Diverse Children's Populations And Their Effects, Brenda Sanchez Vega May 2026

Relationships Between Therapists And Diverse Children's Populations And Their Effects, Brenda Sanchez Vega

Undergraduate Honors Theses

Roughly around 13.8% of children and adolescents in the United States receive some sort of counseling or therapy; the main focus of the research is on racial/ethnic minoritized children. Because APA’s ethical codes don’t address all possible situations, positive client-therapist relationships are bound to develop, including dyads or dual relationships, improving treatment progress. With the United States continuing to grow in diversity, there’s still a big gap in the literature surrounding minority children in therapy. Speculating if therapy approaches are used effectively for all children, including minoritized children. Based on past research already conducted, developing a positive client-therapist dual relationship …

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Molecular Mechanisms Of Pfas Induced Protein Dysfunction: Implications For Neurodegenerative Risk, Daisy Lee Wilson May 2026

Molecular Mechanisms Of Pfas Induced Protein Dysfunction: Implications For Neurodegenerative Risk, Daisy Lee Wilson

Open Access Theses & Dissertations

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants with widespread human exposure and well-established links to adverse health outcomes. However, despite increasing epidemiological and experimental evidence, the molecular mechanisms underlying PFAS-induced neurotoxicity remain incompletely defined. This dissertation investigates PFAS toxicity across biological scales, integrating protein biophysics with neuronal cell models to elucidate pathways relevant to neurodegeneration. At the molecular level, we demonstrate that PFAS disrupts the structure and function of globular proteins, including the impairment of α-lactalbumin's calcium-binding capacity and of β-lactoglobulin's retinol binding, through concentration-dependent conformational destabilization. By applying these findings to a neuronal model, we show that …

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Querida Ana, Lorena Castillo Berrones May 2026

Querida Ana, Lorena Castillo Berrones

Open Access Theses & Dissertations

Querida Ana es una novela epistolar de carácter híbrido que narra la relación entre dos amigas separadas, primero por fronteras geográficas y después por la enfermedad. La historia inicia cuando Carlina emigra a Houston, Texas, para reunirse con su familia, mientras Ana Lucía permanece en San Luis Potosí, México. A través de cartas, postales y mensajes, la obra construye un espacio íntimo donde las protagonistas conversan, crean y sostienen su amistad pese a la distancia. La escritura se convierte en el puente que articula la narración y permite explorar temas como la migración, la frontera, la memoria, la enfermedad y …

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From Covid-19 To Influenza: Transforming Public Health Surveillance Through Freeze-Drying Innovations, Rui Dong May 2026

From Covid-19 To Influenza: Transforming Public Health Surveillance Through Freeze-Drying Innovations, Rui Dong

Open Access Theses & Dissertations

Public health surveillance increasingly relies on wastewater-based epidemiology (WBE) as a non-invasive, community-level early warning system for infectious diseases like COVID-19 and influenza. However, detecting minute viral concentrations in complex wastewater matrices remains a significant analytical challenge. Traditional concentrating methods, such as ultrafiltration, are limited by high performance variability and significant viral signal loss due to necessary pretreatment steps, such as pre-clarification, which inadvertently discard solid-associated viral fractions. To address these limitations, this dissertation introduces and evaluates freeze-drying as a novel, pretreatment-free concentrating method for wastewater viral surveillance. By sublimating water, freeze-drying uniquely retains both liquid and solid-phase viruses within …

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