Digital Commons Network^™

Implementation And Outcomes Of A Digital Onboarding Taskforce In The Acute Care Setting, Julianna Lenoir, Alexzandra Gentsch, Akshay Krishnan, Jeffrey Ndubisi, Marissa Witmer, Kristin L. Rising, Brooke Worster, Angela M. Gerolamo

Jefferson Center for Connected Care Faculty Papers

INTRODUCTION: Use of digital health technology can improve patient health outcomes; however, not all patients have the knowledge and skills to download a health app and access a patient portal. Providing digital onboarding support to hospitalized patients has potential to overcome some barriers to accessing needed education in the community, including both having the time and a location to receive education. To address this, our team developed the Jefferson Digital Onboarding Taskforce (JeffDOT), a group of staff and students who approach hospitalized patients and provide one-on-one teaching on how to sign up for and use a patient portal.

METHODS AND …

Vesicle-Mediated Mitochondrial Clearance Presents An Actionable Metabolic Vulnerability In Triple-Negative Breast Cancer, Jody Vykoukal, Yihui Chen, Mingxin Zuo, Riccardo Ballarò, Monica J Hong, Hansini Krishna, Daniela B Rodriquez-Perera, Hiroyuki Katayama, Ehsan Irajizad, Ranran Wu, Ricardo A León-Letelier, Jennifer B Dennison, Angelica M Gutierrez, Adriana Paulucci-Holthauzen, Timothy C Thompson, Leona Rusling, Yining Cai, Fu Chung Hsiao, Soyoung Park, Banu Arun, Samir Hanash, Johannes F Fahrmann

Faculty, Staff and Student Publications

Selective autophagy of mitochondria is known to promote cancer cell survival and progression, including in triple-negative breast cancer (TNBC). Here, we apply an integrated multi-omics approach together with functional experimental analyses to investigate metabolic adaptations that support mitochondrial quality control in TNBC. We detail a mitochondrial quality control mechanism, complementary to mitophagy, that is enabled by a program of heightened extracellular sphingomyelin salvaging in TNBC coupled with extracellular vesicle-mediated intracellular clearance of mitochondrial damage. Targeting of this onco-metabolic pathway via repurposing of eliglustat, a selective small molecule inhibitor of glucosylceramide synthase, results in ceramide-mediated compensatory mitophagy and cancer cell death …

Accurate Imputation Of Pathway-Specific Gene Expression In Spatial Transcriptomics With Pasta, Ruoxing Li, Peng Yang, Mauro Di Pilato, Jianjun Zhang, Christopher R Flowers, Lulu Shang, Ziyi Li

Faculty, Staff and Student Publications

Mapping the entire transcriptome at single-cell resolution under its natural spatial context is essential for investigating the oncogenesis and progression of diseases. The recently emerged targeted in-situ technologies retain the spatial organization of cells at high resolution, although they remain limited in the number of genes that can be simultaneously measured. To overcome this obstacle, numerous computational methods have been developed to predict unmeasured gene expression in spatial transcriptomics data by leveraging scRNA-seq data. Most of these methods focus on the expression of individual genes and usually generate highly variable predictions. In this study, we introduce PASTA (PAthway-oriented Spatial gene …

Successful Treatment Of Comorbid Sapho Syndrome And Hidradenitis Suppurativa With Upadacitinib, Deepika Narayanan, Tiffaney Tran, Valeria Duque-Clavijo, Hung Q Doan, Stephen K Tyring

Faculty, Staff and Student Publications

No abstract provided.

Lung Adenocarcinoma Surfaceome Remodeling With Egfr Inhibitors Uncovers Placental Alkaline Phosphatase As A Target For Combination Therapy, Yihui Chen, Rongzhang Dou, Monica J Hong, Hanwen Xu, Jody Vykoukal, Ricardo A León-Letelier, Yining Cai, Soyoung Park, Ehsan Irajizad, Fu Chung Hsiao, Jennifer B Dennison, Edwin J Ostrin, Johannes F Fahrmann, Hiroyuki Katayama, Samir M Hanash

Faculty, Staff and Student Publications

Treatment of lung adenocarcinomas (LUADs) that exhibit activated epidermal growth factor receptor (EGFR) with EGFR tyrosine kinase inhibitors (TKIs) has limited efficacy. Assessment of the impact of EGFR TKI on the LUAD surfaceome remodeling reveals potential therapeutic targets. We identify placental type alkaline phosphatase (ALPP), which has restricted expression in normal tissues, among upregulated surface proteins following EGFR TKI treatment of both TKI sensitive as well as resistant cells. EGF treatment represses ALPP expression, whereas EGFR TKIs upregulate its expression through dephosphorylation and activation of FoxO3a, a transcriptional regulator that binds to the promoter region of ALPP. The combination of …

Quantitative Profiling Of Intratumor Immune Heterogeneity Identifies Loss Of Immune Diversity As A Hallmark Of Cancer Progression, Qiqi Lu, Jiangti Luo, Chia-Hao Tung, Xiaosheng Wang, Zhongming Zhao

Faculty, Staff and Student Publications

Immunological intratumor heterogeneity (ImTH) describes the variability in the types, spatial distribution, and functional states of immune cells within tumors. While evidence suggests that ImTH influences tumor progression and therapeutic response, few studies have provided a quantitative characterization of ImTH. Here, we present Scoring Immunological Intratumor Heterogeneity (ScImTH), a novel algorithm that quantifies ImTH by calculating the Shannon entropy of immune cell type proportions within the tumor microenvironment. Using bulk, single-cell, and spatial transcriptomic datasets, we show that reduced ScImTH scores are associated with unfavorable survival outcomes, tumor progression-related molecular and phenotypic features, immunosuppressive states, and resistance to immunotherapy across …

The Inferred Functional Connectome Underlying Circadian Synchronization In The Mouse Suprachiasmatic Nucleus, K L Nikhil, Bharat Singhal, Daniel Granados-Fuentes, Jr-Shin Li, István Z Kiss, Erik D Herzog

2020-Current year OA Pubs

Circadian rhythms in mammals arise from the spatiotemporal synchronization of ~20,000 neuronal clocks in the suprachiasmatic nucleus (SCN). Although anatomical, molecular, and genetic approaches have revealed diverse SCN cell types, how network-level wiring enables their synchronization remains unclear. To overcome the challenges of inferring functional connectivity from fixed tissue, we developed Mutual Information & Transfer Entropy (MITE), an information-theoretic framework to infer directed cell-cell connections with high fidelity from long-term live-cell imaging. Recording and analyzing 3,290 h of clock gene expression from 8,261 SCN neurons across 17 mice, we uncovered a highly conserved, sparse SCN network organized into two asymmetrically …

Wdfy4-Dependent Cross-Presentation Proceeds Via A Vacuolar Antigen-Processing Route, J Luke Postoak, Alyssa Koch, Sicong Shan, Ray A Ohara, Suin Jo, Jing Chen, Pritesh Desai, Feiya Ou, Magdalena Kraft, Michael S Diamond, Sunkyung Kim, Theresa L Murphy, Kenneth M Murphy

2020-Current year OA Pubs

The intracellular processing route used by type I conventional dendritic cells (cDC1) for cross-presentation of viral- or tumor-associated antigens remains controversial. One model proposes that captured antigens exit from damaged phagolysosomes and enter the cytosol, where they are processed for presentation by MHC class I molecules (MHC-I). This model relies on proteasomal degradation and TAP-dependent peptide transport into the endoplasmic reticulum (ER) for loading by the peptide loading complex (PLC). An alternative model proposes a vesicular route in which captured antigens are retained and processed within an endocytic compartment. A compelling argument favoring the cytosolic model is the dramatic loss …

William Welling Werntz [Inducted 1968], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Yuji Ijiri [Inducted 1989], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Norton Moore Bedford [Inducted 1988], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Philip Leroy Defliese [Inducted 1987], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Oscar Strand Gellein [Inducted 1985], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Lord Henry Alexander Benson [Inducted 1984], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Sidney Davidson [Inducted 1983], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Herbert Elmer Miller [Inducted 1982], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Elmer Boyd Staats [Inducted 1981], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Marshall Smith Armstrong [Inducted 1980], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Maurice Moonitz [Inducted 1979], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Howard Irwin Ross [Inducted 1977], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

John William Queenan [Inducted 1976], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Leonard Paul Spacek [Inducted 1975], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Robert Martin Trueblood [Inducted 1974], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

John Lansing Carey [Inducted 1968], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

George Davis Bailey [Inducted 1968], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

James Loring Peirce [Inducted 1965], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Perry Empey Mason [Inducted 1964], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Paul Franklin Grady [Inducted 1964], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Lloyd Morey [Inducted 1963], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.

Andrew Barr, Jr. [Inducted 1963], American Accounting Association

Accounting Hall of Fame Brochures

No abstract provided.