Antioxidant Properties Of N-Hexane Extract From Tobacco Leaves (Nicotiana Tabacum L.) Using 2,2-Diphenyl-1-Picrylhydrazil (Dpph) Method, Vinda M. Patricia, Dhea K. Az-Zahra, Taufik M. Fakih, Farendina Suarantika

Pharmacology and Clinical Pharmacy Research

Tobacco (Nicotiana tabacum L.) is a commercial plant that often used for making cigarettes. In 2012, the government issued regulations for the diversification of tobacco products besides cigarettes. Some of these diversified tobacco products include organic pesticides, anesthetics, cosmetic ingredients, and biochar as an alternative to coal. Therefore, in order to further to diversification of tobacco product, this study aimed to determine the antioxidant activity from n-hexane extract from leaves using the Soxhlet extraction method. Antioxidant activity was assessed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, while the total phenolic content was determined using the Folin-Ciocalteu method that allowed quantification of phenolic …

Minority Female Political Representation In Westchester County Politics: A View From Minority Female Elected Office Holders, Dwanita Michelle Crosby

Walden Dissertations and Doctoral Studies

African American women have historically been underrepresented in political leadership, a disparity largely attributed to the persistent absence of structured support systems—particularly mentorship—that promote advancement across federal, state, and local levels. Existing literature highlights gender and racial disparities in political trajectories but often overlooked the specific role of mentorship in shaping leadership outcomes for African American women at the municipal level. This gap was particularly critical given the increasing civic engagement and influence of African American women in local governance. The purpose of this general qualitative study was to examine how mentorship shaped the political journeys of African American women …

Phytochemical And Nutritional Composition Of Vernonia Amygdalina: Antioxidant, Antidiabetic Efficacy, And Hematological Benefits, Hassan A. Adewuyi, Atapia I. Messiah, Ochonung E. Ogar, Waheed S. Adio, Adebimpe H. Aluwatoyin, Obunadike Obunadike, Adeleye A. Edema, Samad Hussein, Usman A. Mohammed, Kevin L. Sebastine, Tolulope O. Jaiyeola

Pharmacology and Clinical Pharmacy Research

Vernonia amygdalina, a traditionally used medicinal plant, has been purported to possess health-promoting properties.The aim of this study is to investigate the phytochemical and nutritional composition of V. amygdalina, evaluate its antioxidant and antidiabetic efficacy, and assess its hematological benefits. Phytochemical screening, proximate analysis, antioxidant assays (DPPH, ABTS), antidiabetic evaluations in streptozotocin-induced diabetic rats, and hematological assessments were conducted according to standard protocol. V. amygdalina exhibited a rich phytochemical profile, comprising flavonoids, phenolic acids, and terpenoids, alongside significant antioxidant activity (IC50 = 12.5 μg/mL) and complete source of protein. Proximate analysis revealed high protein (21.1%) and fiber (15.6%) content. Antidiabetic …

Rna Sequencing And Immunohistochemistry Jointly Improve Tumor Biomarker Interpretation, Vladimir Kushnarev, Danil Stupichev, Suren Davitavyan, Kirill Kriukov, Basavaraja U Shanthappa, Anna Butusova, Sofia Menshikova, Anna Belozerova, Anastasia Shvyrkova, Arina Tkachuk, Olga Khatenkova, Linda Balabanian, Ekaterina Postovalova, Jochen K Lennerz, Funda Meric-Bernstam, Alexander Bagaev

Faculty, Staff and Student Publications

This study aimed to assess the correlation between RNA sequencing (RNA-seq) and immunohistochemistry (IHC) in detecting key cancer biomarkers across solid tumors, and then, to establish RNA-seq thresholds that accurately reflect clinical IHC classifications. Expression levels of nine biomarkers-ESR1, PGR, AR, MKI67, ERBB2, CD274, CDX2, KRT7, and KRT20-were analyzed in 365 formalin-fixed, paraffin-embedded samples from breast, lung, gastrointestinal, and other solid carcinomas. Correlations between RNA-seq data and IHC scores were determined using Spearman's correlation coefficients, with RNA-seq cut-offs established to distinguish positive from negative IHC scores. The results revealed strong correlations for most biomarkers, with coefficients ranging from 0.53 to …

Rsph4a-Pcdx: An Index To Predict Lung Function Decline In Primary Ciliary Dyskinesia, Gabriel Román-Ríos, Gabriel Rosario-Ortiz, Marcos J Ramos-Benitez, Ricardo A Mosquera, Wilfredo De Jesús-Rojas

Faculty, Staff and Student Publications

Primary ciliary dyskinesia (PCD) is a rare, genetically heterogeneous disorder that impairs mucociliary clearance and leads to progressive lung disease. This study aimed to characterize lung function decline in a genetically homogeneous cohort of Puerto Rican patients with

Quantile Index Predictors Using R Package Hyper.Gam, Tingting Zhan, Misung Yi, Inna Chervoneva

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

MOTIVATION: Evaluation of single-cell protein expression from immunohistochemistry images is used increasingly in biomedical research. Many proteins are used solely for phenotyping cells in the tumor microenvironment. Other proteins with meaningfully quantitative expression levels provide so-called functional protein biomarkers. There is still a limited number of methods and software tools available for utilizing the entire distributions of single-cell expression levels.

RESULTS: We present the R package hyper.gam, providing a supervised learning framework for deriving biomarkers based on single-cell distribution quantiles. The single-cell data are first converted into sample quantile functions, which are then used as predictors in scalar-on-function regression models …

Impact Of Firefighters' Work Schedule On Cardiovascular Disease Risk, Wellbeing And Safety In Firefighters: Protocol For The Swift Study, A Multidisciplinary Prospective And Cross-Sectional Study, Walaa F. Abdelmoaty, Shelby L. Watkins, Aanuoluwakiitan Ayeni, David A. Hurtado, Andrew W. Mchill, Jeremy J. Biggs, Kurt T. Hegmann, Steven A. Shea, Todd Bodner, Nicole P. Bowles

Psychology Faculty Publications and Presentations

Firefighters face frequent physical and psychosocial stressors, increasing their risk for hypertension. Rising call volumes with a stable workforce have heightened occupational burdens. To meet their occupational demands while increasing time off-duty, fire departments across the country have switched from a 24 hours on 48 hours off (termed ‘24/48’) work schedule to one that increases the number of consecutive days off (eg, 1 day on, 3 days off, 2 days on, 3 days off (termed ‘1/3/2/3’) or 48 hours on 96 hours off (termed ‘48/96’)). However, these schedule changes come at the expense of increasing time on-duty, which may have …

Inhibition Of Epithelial Cell Yap-Tead/Lox Signaling Attenuates Pulmonary Fibrosis In Preclinical Models, Darcy Elizabeth Wagner, Hani N Alsafadi, Nilay Mitash, Aurelien Justet, Qianjiang Hu, Ricardo Pineda, Claudia Staab-Weijnitz, Martina Korfei, Nika Gvazava, Kristin Wannemo, Ugochi Onwuka, Molly Mozurak, Adriana Estrada-Bernal, Juan Cala-Garcia, Katrin Mutze, Rita Costa, Deniz Bölükbas, John Stegmayr, Wioletta Skronska-Wasek, Stephan Klee, Chiharu Ota, Hoeke A Baarsma, Jingtao Wang, John Sembrat, Anne Hilgendorff, Jun Ding, Andreas Günther, Rachel Chambers, Ivan Rosas, Stijn De Langhe, Naftali Kaminski, Mareike Lehmann, Oliver Eickelberg, Melanie Königshoff

Faculty, Staff and Students Publications

Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal disease characterized by excessive extracellular matrix deposition. Current IPF therapies slow disease progression but do not stop or reverse it. The (myo)fibroblasts are thought to be the main cellular contributors to excessive extracellular matrix production in IPF. Here we show that fibrotic alveolar type II cells regulate production and crosslinking of extracellular matrix via the co-transcriptional activator YAP. YAP leads to increased expression of Lysl oxidase (LOX) and subsequent LOX-mediated crosslinking by fibrotic alveolar type II cells. Pharmacological YAP inhibition via verteporfin reverses fibrotic alveolar type II cell reprogramming and LOX …

Sporadic Als Induced Pluripotent Stem Cell Derived Neurons Reveal Hallmarks Of Tdp-43 Loss Of Function, Jeffrey D Rothstein, Olivia Keeley, Caroline Warlick, Timothy M Miller, Cindy V Ly, Jonathan D Glass, Alyssa N Coyne

2020-Current year OA Pubs

Nuclear loss and cytoplasmic buildup of the RNA-binding protein TDP-43 is a hallmark of ALS and related disorders. While studies using artificial TDP-43 depletion in neurons have revealed changes in gene expression and splicing, their relevance to actual patients remained unclear. Induced pluripotent stem cell (iPSC)-derived neurons (iPSNs) from 180 individuals, including controls, C9orf72 ALS/FTD, and sporadic ALS (sALS) patients were used to generate and analyze ~32,500 qRT-PCR data points across 20 genes which identified variable, time-dependent signatures of TDP-43 loss of function in individual lines. Notably, the same changes were also seen in postmortem brain tissue from the same …

Sex, Stress And Hormones : The Role Of Modifiable Risk Factors In The Kinetics Of Alzheimer’S Disease, Hannah Marie Edwards

Arts & Sciences Graduate Student Theses and Dissertations

Alzheimer’s Disease (AD) is a neurodegenerative disease hallmarked by the presence of amyloid- plaques and tau tangles in the brain. The process of the disease begins decades prior to the onset of symptoms and is thought to be driven first by a buildup of amyloid- peptide in the extracellular space, triggering a cascade of pathology and symptomology culminating in loss of cerebral volume and cognitive impairment. Women are diagnosed with AD at a rate 2/3 higher than their male counterparts, even when controlling for age. While most driving factors of the disease are thought to be genetic, roughly 40% of …

Does Woody Encroachment In Eastern Iowa Crp Fields Depend On Age Or Surrounding Forest Cover?, Drew Denotter, Tyler Evans, Laura Jackson

Summer Undergraduate Research Program (SURP) Symposium

Historically, tallgrass prairie covered approximately 85% of Iowa’s landscape. Today, Iowa has experienced the most extensive land conversion and destruction of native habitats, with nearly all of the biodiversity from the native prairie converted to private cropland. This transformation has led to declines in water and soil quality, as well as losses in plant and animal genetic diversity. The Conservation Reserve Program (CRP) incentivizes farmers to remove land from production and restore it to tallgrass prairie, but these restorations face a growing threat: woody encroachment. Invasive woody species can expand rapidly, with densities increasing over a relatively short period of …

Implications Of Plant Clonality For Nutrient Pollution In Grasslands And Wetlands, Sydney Aird, Kenneth Elgersma

Summer Undergraduate Research Program (SURP) Symposium

No abstract provided.

Whole Genome Sequencing Of Bumblebees With Varied Tissue Quantity, Extraction Methods, And Bioinformatic Inquiries, Halle Hoefing, Ai Wen

Summer Undergraduate Research Program (SURP) Symposium

Bumblebee populations are a key source of pollination in many local prairies across Iowa, and genetic diversity among these populations is essential to their health. In order to study the variation, it needs to be examined at the genomic level. However, the current method for bumblebee DNA extraction is lethal, and involves removing tissue from the thorax of captured bees. To find a non-lethal method, procedures were adapted from reference literature that used tarsal clippings of bees rather than capturing and freezing the entire bee. DNA was extracted from 18 previously captured Bombus impatiens individuals, among which four bees were …

Developing A Dna Extraction Method For Mineral Abundant Limestone Cave Samples, Treytun Garcia, Marek Sliwinski

Summer Undergraduate Research Program (SURP) Symposium

Caves offer a unique environment to study microbial life that survives under extreme nutrient limitation. To this extent, 16s metabarcoding serves as a sort of “DNA fingerprint” that allows us to identify and study the microbial composition of these environments, but there are hurdles that must be overcome to obtain enough DNA for effective and reliable 16s sequencing. It is important to maximize the amount of DNA that can be extracted from the Wind Cave samples because it is an extremely low biomass environment and sufficient DNA for sequencing can be hard to get. Wind Cave National Park serves as …

Breeding Season Bird Use Of And Non-Native Plants Found In Food Plots In The Southern Appalachian Mountains, Nathan A. Klaus, Kirk Stodola

Georgia Journal of Science

Traditional agricultural style food plots, or wildlife openings, are widely used for wildlife management and hunting opportunities on public and private lands in the Southeastern United States. These habitats are often cited as beneficial for game and nongame wildlife, but there are few published studies examining nongame bird use of food plots in recent decades. Modern concerns about the ecological costs of food plots, particularly fragmentation and invasive non-native species, warrant a new examination of this practice. We conducted avian surveys during the breeding season of 2008 at 39 sites composed of three different treatments: traditional food plots, novel food …

Reimagining Learning Outdoors: How Active Outdoor Play-Based Learning (Aopl) Supports Student Growth And Academic Success - A White Paper For School Leaders And Classroom Teachers, Steph Dean

Publications

Active Outdoor Play-Based Learning (AOPL) is an evidence-based approach that integrates active play, outdoor environments, and academic goals. It supports not only curricular learning but also students’ physical, emotional, social, and cognitive development. This white paper shares practical insights from a systematic review of 21 empirical studies (2008-2024) on AOPL implementation in formal school settings.

AOPL is a pedagogical strategy that uses play and the outdoors as meaningful contexts for learning. Teachers who implement AOPL build connections between curriculum and students’ natural curiosity while leveraging the benefits of being outside¹.

This paper highlights practical strategies and compelling outcomes …

Barriers In Trauma-Responsive School Implementation: A Study Exploring And Comparing Rural, Suburban, And Urban Districts In Indiana, Allison L. Makowski

All-Inclusive List of Electronic Theses and Dissertations

Trauma responsive practices are a type of educational practice currently being utilized in our schools. Our students come to school every day with some type of trauma which is a negative event that a child experiences. Types of traumatic experiences could be in the form of a natural disaster, car accident, or some type of abuse. The current study examined barriers that schools experience when implementing trauma responsive practices. The schools were examined further to investigate whether or not there were differences in barriers in rural, suburban, and urban school districts in the state of Indiana.

Gender Role Conflict In Women, Candyletta Lewis

All-Inclusive List of Electronic Theses and Dissertations

This study examines gender role conflict (GRC) in women within the context of feminine norms, with a focus on developing a scale designed to measure GRC derived from feminine ideology that may contribute to gender related stress. The primary research questions address the factor structure, reliability, and validity of the Gender Role Conflict Scale for Women (GRCS-W). Additional research questions examine the relationship between feminine gender role conflict and psychological well-being, and whether any racial differences exist in self-reported GRC. Participants include 132 women aged 18–70 from diverse educational backgrounds. Findings revealed a three-component solution for the GRCS-W. The internal …

A Convergent Parallel Mixed Method Design Analysis Of Sponsorship And The Advancement Of Women In The Workplace, Shaparak Malek-Morvai

Masters Theses & Specialist Projects

Sponsorship is vital for advancing women's careers but is often inconsistently applied. Limited programs for women seeking leadership roles and a lack of sponsors can hinder progress. This study used a mixed-methods approach, combining quantitative survey data from 73 participants with qualitative insights. Sponsors advocate for protégés, emphasizing visibility, promotion, and networking, while mentorship offers guidance and coaching. Many leadership roles confuse sponsorship with mentorship, as noted by Helms et al. (2016). However, the key difference lies in influence. The study had four objectives: to explore women's perceptions of sponsorship, analyze how roles and tenure influence these perceptions, enhance discussions …

Tides, Tourists, And Tails: Exploring Human-Wildlife Overlap On The Homer Spit, Alaska, Serena Seiler

Masters Theses & Specialist Projects

Coastal areas present unique challenges for human-wildlife coexistence due to their shared use by people and marine species. The Homer Spit in Homer, AK (59.6481° N, 151.5299° W) is a projection of land into Kachemak Bay on which lodging, restaurants, campgrounds, RV parks, fishing, and a variety of coastal activities exist or occur, especially during the summer months. Two of the most popular nearshore marine mammals that attract visitors are sea otters (Enhydra lutris) and harbor seals (Phoca vitulina). These two species spend time swimming, foraging, and resting around the Spit, potentially resulting in co occurrences with humans. Using a …

“Literally Made Me Cry”: The Impact Of Course Material Costs On University Of Kentucky Students, Stephen Krueger

Library Reports and White Papers

The Student Course Materials Cost Survey was administered September 16 – October 6, 2024. It was open to current students at the University of Kentucky (UK). The goals were to help UK Libraries better understand how the cost of course materials affects students at UK and to provide a snapshot of student spending across the university. The questions were based on similar research done at other institutions in Virginia (Virginia Course Materials Survey, 2021) and Florida (2022 Student Textbook and Instructional Materials Survey, 2022). Other work based on those surveys includes the 2023 Illinois Course Materials Survey (CARLI, 2023) and …

Rangelands Glossary, Department Of Primary Industries And Regional Development, Western Australia

Natural resources factsheets

An alphabetical list of common Rangelands terminology.

Joint Kinematics And Kinetics During Vertical Jump Landings With Progressively Increasing External Loads, Darcie L. Yount

Dissertations

This study examined sagittal and frontal plane kinematics and kinetics during countermovement jump (CMJ) landings performed with either a traditional barbell (BB) or a hexagonal bar (HEX) across increasing submaximal loads in recreationally active males. Generally, hip and knee flexion angles and joint mean angular velocities decreased as loads increased. Hip extension and knee flexion moments increased as load increased up to 20 kg, at which point these values decreased. Performing the CMJ with the HEX exhibited increased frontal plane stability compared with the BB group. Additionally, the BB group exhibited stiffer landing strategies, with decreased sagittal plane joint angles …

Aging Is Not All Doom And Gloom: How Do Beliefs About Aging Moderate The Effects Of Mortality Salience On Ageism Attitudes?, Kristen Schwindt Edmondson

Masters Theses & Specialist Projects

The purpose of the study is to examine how young adults’ beliefs about aging moderate the effects of mortality salience on their ageism attitudes. Past research suggests that ageism is, in part, due to the presence of elderly people reminding people of their own aging process and mortality (Martens et al., 2004). Little is known, however, about how individuals’ beliefs about aging may moderate the impact of mortality salience on their attitudes toward aging. Some people tend to perceive aging-related changes as relatively fixed (i.e., essentialist belief), whereas others perceive them as relatively malleable (nonessentialist belief). Would a more malleable …

Smokejumper Biographies For 1963 Rookie Class, National Smokejumper Association

Smokejumper Biographies by Rookie Year

This document contains short biographies of smokejumpers who rookied in 1963.

Perspectives On Water Policies And Alternative Land Uses Among Alfalfa Farmers In The Great Salt Lake Basin, Bryn Watkins, Stacia Ryder

Publications

Climate change threatens many resources necessary for life. In arid and semiarid regions like Utah, water represents one of the key climate-related conflicts (1,2). In Utah, a changing climate paired with human overconsumption has contributed to the drying of the Great Salt Lake. Rising temperatures will lead to greater evapotranspiration and decreasing headwater streamflow and groundwater storage, resulting in increasing dryness in the Great Salt Lake Basin regardless of human water consumption (3). This in turn threatens the ecosystems, economies, and cultures of the Great Salt Lake Basin, accelerating the local impacts of climate change (4).

Fisheries Research Report No. 353: Ecological Risk Assessment For The North Coast Demersal Scalefish Resource, Kimberley A. Smith, Brett Crisafulli, Gabby E. Mitsopoulos, Fabian I. Trinnie, Taylor Grosse, T Thompson, Stephen J. Newman

Fisheries Research Reports

On the 26 March 2025, the Department of Primary Industries and Regional Development (DPIRD) convened an ecological risk assessment (ERA) of the fisheries that access the North Coast Demersal Scalefish Resource (Resource). This Resource comprises a large number of tropical demersal scalefish species that occur in waters of the North Coast Bioregion, including tropical snappers, emperors and cods.

This document contains the results of the ERA along with the background information used to support the risk scoring process. This includes an overview of the Western Australian commercial fisheries that access the Resource, namely the Pilbara Fish Trawl Interim Managed Fishery, …

Smokejumper Biographies For 1962 Rookie Class, National Smokejumper Association

Smokejumper Biographies by Rookie Year

This document contains short biographies of smokejumpers who rookied in 1962.

Review: Wombs Of Empire: Population Discourses And Biopolitics In Modern Japan, By Sujin Lee Available, Alexander Bay

History Faculty Articles and Research

A review of Wombs of Empire: Population Discourses and Biopolitics in Modern Japan by Sujin Lee.

A Quality Improvement Project To Improve Touch Exposures In The Neonatal Intensive Care Unit., Jeremi Tricia Braga

Doctor of Nursing Practice Papers

Introduction: Premature infants in the NICU are exposed to many noxious stimuli that could cause negative effects on development. The SENSE® program has been developed to provide developmentally appropriate sensory stimulation for neonates. This project will focus on sense of touch primarily with KC or also referred to as skin-to-skin.

Environment: This QI project took place in to a Level III NICU at a tertiary medical center in Louisville, Kentucky. The facility has a total of 421 beds including the NICU that is licensed for 34 beds and caring for infants born prematurely as early as 22 weeks to term …