Digital Commons Network^™

Click-Ready Gold Nanoparticles From Aqueous Mechanochemistry: 2-Propynylamine As A Reducing Agent And Surface Ligand, Amber L. Garcia, Brian S. Mitchell, Amanda Reusch, Mark J. Fink, Juan P. Hinestroza, Yelin Ko, Julie P. Vanegas

Physics & Astronomy Faculty Publications

We report a rapid aqueous method for synthesizing monodisperse gold nanoparticles (AuNPs), employing 2-propynylamine as both an intrinsic reducing agent and a surface-stabilizing ligand. This self-mediated process—achieved in a single step—yields spherical AuNPs with an average diameter of 4.0 ± 1.0 nm and a well-defined localized surface plasmon resonance band centered at 520 nm. Acting as a bifunctional molecule, 2-propynylamine simultaneously reduces HAuCl4·3H2O to elemental gold and passivates the nanoparticle surface through coordination via the amine group, while preserving a terminal alkyne (–C≡CH) functionality. This reactive moiety remains exposed and chemically accessible, enabling post-synthetic modification through Cu(I)-catalyzed azide–alkyne cycloaddition. Control …

Hypoxia Exposure Fine-Tunes Mitochondrial Function In Sea Turtle Cells, B. Gabriela Arango, David C. Ensminger, Dianna Xing, Céline A. Godard-Codding, José Pablo Vázquez-Medina

Faculty Research, Scholarly, and Creative Activity

Abstract: Sea turtles experience extreme fluctuations in oxygen levels derived from extended breath-hold diving, yet the cellular adjustments underlying hypoxia tolerance in these animals remain poorly understood. Here, we employed metabolite profiling, extracellular flux assays and microscopy analyses of the mitochondrial reticulum to investigate how primary cells derived from sea turtles and lizards cope with extended hypoxia exposure. Cells from both species proliferate in primary culture, stain positive for fibroblast markers, are metabolically active and stabilize HIF1-α when exposed to chemical or environmental hypoxia. In contrast to lizard cells, sea turtle cells exhibit a faster and more robust response to …

Association Of Genetic Scores Related To Insulin Resistance With Neurological Outcomes In Ancestrally Diverse Cohorts From The Trans-Omics For Precision Medicine (Topmed) Program, Chloé Sarnowski, Yixin Zhang, Farah Ammous, Lincoln M P Shade, Daniel Dicorpo, Xueqiu Jian, Donna K Arnett, Thomas R Austin, Alexa Beiser, Joshua C Bis, John Blangero, Eric Boerwinkle, Jan Bressler, Joanne E Curran, Charles S Decarli, Harsha Doddapaneni, Josée Dupuis, David W Fardo, Jose C Florez, Stacey Gabriel, Richard A Gibbs, David C Glahn, Namrata Gupta, Hector M González, Kevin A González, Konstantinos Hatzikotoulas, Kathleen M Hayden, Susan R Heckbert, Bertha Hidalgo, Alicia Huerta-Chagoya, Timothy M Hughes, Sharon L R Kardia, Charles L Kooperberg, Lenore J Launer, W T Longstreth, Ravi Mandla, Rasika A Mathias, Andrew P Morris, Thomas H Mosley, Ilya M Nasrallah, Paul Nyquist, Bruce M Psaty, Qibin Qi, Laura M Raffield, Nigel W Rayner, Alexander P Reiner, Claudia L Satizabal, Elizabeth Selvin, Magdalena D R Sevilla-Gonzalez, Albert V Smith, Jennifer A Smith, Kirk Smith, Beverly M Snively, Lorraine Southam, Tamar Sofer, Ken Suzuki, Henry J Taylor, Miriam S Udler, Karine A Viaud-Martinez, Sylvia Wassertheil-Smoller, Alexis C Wood, Lisa R Yanek, Xianyong Yin, Alisa K Manning, Jerome I Rotter, Stephen S Rich, James B Meigs, Myriam Fornage, Sudha Seshadri, Alanna C Morrison, Topmed Neurocognitive Working Group, Topmed Diabetes Working Group

The Brown Foundation: Institute of Molecular Medicine

To better characterize the potential biological mechanisms underlying insulin resistance (IR) and dementia, we derive cross-population and population specific polygenic scores [PSs] for fasting insulin and IR-related partitioned PSs [pPSs]. We conduct a cross-sectional study of the associations of these genetic scores with neurological outcomes in >17k participants (36% men, mean age 55 yrs) from the Trans-Omics for Precision Medicine (TOPMed) program (50% Non-Hispanic White, 23% Black/African American, 21% Hispanic/Latino American, and 4% Asian American). We report significant negative associations (P <  0.002) of the cross-population (P = 1.3 × 10-5) and European (PEA = 3.0 × 10-8) fasting insulin PSs with total cranial volume, and of a metabolic syndrome European PS with general cognitive function (BEA = -0.13, PEA = 0.0002) and lateral ventricular volume (BEA = 0.09, PEA = 0.002). We identify suggestive negative associations (P <  0.007) of metabolic syndrome and obesity pPSs with general cognitive function, and of lipodystrophy pPSs with total cranial volume. A higher genetic predisposition to IR is associated with lower brain size, and a genetic predisposition to specific IR-related type 2 diabetes subtypes, such as metabolic syndrome and mechanisms of IR mediated through obesity and lipodystrophy, is potentially involved in cognitive decline.

Site-1 Protease Is A Negative Regulator Of Sarcolipin Promoter Activity, Isha Sharma, Meredith O Kelly, Katelyn Hanners, Ella S Shin, Muhammad G Mousa, Shelby Ek, Gretchen A Meyer, Rita T Brookheart

2020-Current year OA Pubs

The timed contraction and relaxation of myofibers in tissues such as the heart and skeletal muscle occur via the tightly regulated movement of calcium ions into and out of the sarcoplasmic reticulum (SR). In skeletal muscle, this phenomenon enables humans to exercise, perform day-to-day tasks, and to breathe. Sarcolipin, a small regulatory protein, prevents calcium ions from entering the SR by binding to and inhibiting SERCA, contributing to myofiber contraction. Disruptions in sarcolipin (SLN) expression are implicated in the pathophysiology of obesity and musculoskeletal disease. However, the mechanisms regulating sarcolipin expression are not clearly understood. We recently showed that site-1 …

Pediatric Heart Failure: A Practical Guide For Primary Care Providers Supporting Families Across The Care Continuum, Shahnawaz Amdani, Kriti Puri, Julie Glickstein, Joseph A Spinner, Jonathan N Johnson, Ashraf S Harahsheh, Majd Makhoul, Susan Denfield

Faculty, Staff and Students Publications

Background/Objectives: Pediatric heart failure (HF) is a complex clinical syndrome with diverse etiologies and presentations. Primary care providers (PCPs) are critical in early recognition, family education, and coordination of ongoing care in collaboration with pediatric cardiologists. This review provides a comprehensive guide for PCPs to understand the causes, symptoms, treatments, and longitudinal considerations for children with HF.

Methods: We conducted a narrative review synthesizing published evidence and expert consensus guidelines, focusing on the clinical presentation, treatment options, and longitudinal care strategies in children with HF.

Results: HF in children most commonly results from congenital heart disease and …

Lung Nr3c1+ And Cxcr6high T Cells Distinguish Immunopathogenesis Of Human Emphysema, Yun Zhang, Maor Sauler, David B Corry, Scott A Ochsner, Sarah Perusich, Li-Zhen Song, Joshua Malo, Raul San Jose Estepar, Francesca Polverino, Farrah Kheradmand

Faculty, Staff and Students Publications

There is a significant knowledge gap in how T cells promote emphysema in smokers with chronic obstructive pulmonary disease (COPD). Single-cell RNA sequencing (scRNA seq) analysis of human samples and relevant clinical data can provide new mechanistic insights into disease pathogenesis. We generated a human lung scRNA seq dataset with extensive disease characteristic annotation and analyzed a second independent scRNA seq dataset to examine the pathophysiological role of T cells in emphysema. Comparisons of pulmonary immune landscapes in emphysematous (E)-COPD, non-emphysematous (NE)-COPD, and control showed positive enrichment of T cells in E-COPD. Pathway analyses identified upregulated inflammatory states in CD4 …

Biologic Management In Severe Asthma For Adults: An American College Of Chest Physicians Clinical Practice Guideline, Amber J Oberle, Farrukh Abbas, Muhammad Adrish, Ioana Agache, Megan Conroy, Angel Coz, Frederic F Little, Manoj J Mammen, Mahesh Padukudru Anand, Raju Reddy, Neha Solanki, Fernando Holguin

Faculty, Staff and Students Publications

Background: Severe asthma affects 5% to 10% of asthma patients but constitutes close to one-half of the medical costs related to asthma due to higher morbidity and health care utilization. Biologic agents have become a standard of care in those unresponsive to standard treatments yet the choice of biologic agent is complex due to the varying mechanisms of action, efficacies, and lack of head-to-head comparisons. Therefore, clinicians need further clinical guidance to optimize their use.

Study design and methods: Panelists developed key clinical questions utilizing the PICO (population, intervention, comparator, and outcome) format to address choice of a biologic agent …

Green Synthesis Of Copper And Silver Nanostructured Particles From Eremurus Plant Extract And Comparison Of Their Optical Properties And Antibacterial Activities, Doaa Ayad Kamil, Ali F. Al-Rawaf, Mohammed Yarub Hani, H.H. Obeed, Tabarek Falah Deindee, Mohammed Ridha Shaeed

Karbala International Journal of Modern Science

In the present investigation, silver (Ag) and copper (Cu) nanostructured particles were synthesized from Eremurus plant by means of an environmentally benign methodology in which plant extracts served as both reducing and stabilizing agents. The resultant nanostructured particles were subjected to characterization techniques, including X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), field-emission scanning electron microscopy (FESEM), and energy-dispersive X-ray spectroscopy (EDXS). XRD analysis confirmed development of Ag nanostructured particles with face-centered cubic (fcc) structure and also Cu nanostructured particles with cubic form. Moreover, the average crystallite dimensions obtained for Ag and Cu were 18.3 nm and 65.7 nm, …

Fabrication And Characterization Of Epoxy Resin Co-Doped With 2,5-Diphenyloxazole And Cerium Fluoride Nanoparticles For Radiation Detection, Akapong Phunpueok, Jaruwan Seangrit, Sarawut Jaiyen, Krittiya Sreebunpeng, Wuttichai Chaiphaksa, Kewalee Nilgumhang, Voranuch Thongpool

Karbala International Journal of Modern Science

This paper presents the fabrication and characterization of a plastic scintillator prepared from epoxy resin doped with 2,5-diphenyloxazole (PPO) and cerium fluoride nanoparticles (CeF₃ NPs) for radiation detection. The CeF₃ NPs were prepared by a chemical process and examined by X-ray diffraction (XRD) and scanning electron microscopy (SEM); it was found that the prepared particles were true CeF₃ NPs with an average particle size of approximately 17 nm. The CeF₃ NPs were co-doped with PPO into epoxy resin and formed into a plastic scintillator of 3 cm in diameter and 2 cm in length. Analysis of …

Healthy Aging And Pet Caretaking: Findings From The Health And Retirement Study, Beth A. Pratt, Erika Friedmann

International Nursing Research Congress (INRC)

The global population of 1 billion people ≥ age 60 is expected to double by 2050, likely increasing the prevalence of age-related cognitive decline (Alzheimer’s Association, 2019). Examining biological, psychological, and social (biopsychosocial) factors and possible moderation by other critical factors, including neighborhood physical disorder (i.e., graffiti, litter, vandalism, safety) and cohesion (i.e., mutual trust/solidarity between neighbors), may inform development of targeted interventions to improve biopsychosocial and cognitive health. Human-animal interaction (HAI) has been found to improve health outcomes and may be a viable option to enhance biopsychosocial factors and delay cognitive decline in older adults (Gee et al., 2017). …

An Observational Study Of Personalized Mhealth To Promote Wellness In Healthcare Workers, Mary S. Mccarthy

International Nursing Research Congress (INRC)

Background: Healthcare workforce resilience weakened during and after the pandemic. Nutrition, physical activity, and sleep deficits further diminish health status. Providing just-in-time resources may promote healthier behaviors in direct-care hospital staff. We had two study aims: 1) coach healthcare workers (HCWs) to make lifestyle changes that enhance wellness, and 2) compare high and low engagement with mHealth resources to achieve recommendations for cardiometabolic health.

Methods: In this 1-year prospective observational study, HCWs had 24/7 access to a dashboard built for personalized health messages and wore an activity and sleep tracker continuously. On-demand UVB phototherapy was available to boost vitamin D …

The Effects Of Lactation Practices On Lactational Amenorrhea: A Systematic Review, Helen Baker, Anna Newton-Levinson, Sharon Leslie

International Nursing Research Congress (INRC)

Background: Lactational amenorrhea (LA) is a contraceptive method that suppresses ovulation postpartum through frequent and exclusive breastfeeding, with up to 98% effectiveness in the first six months if practiced optimally (Curtis et al., 2024). LA relies on consistent breastfeeding intervals and nipple stimulation, which inhibit gonadotropin-releasing hormone (GnRH) secretion (McNeilly, 2001). Factors such as supplemental feeding, breast pump use, and early return to work may reduce LA's efficacy (Stuebe & Chen, 2022). This review assesses the impact of various lactation practices on LA to provide current insights and support informed counseling on breastfeeding as a contraceptive method.

Methods: Following PRISMA …

A Mixed Method Study On Nurses Pain Management For Hospitalized Persons Living With Dementia, Beverley F. Ingelson

International Nursing Research Congress (INRC)

Purpose Statement: To assess factors influencing nurses’ beliefs and knowledge when assessing and managing pain in hospitalized older persons living with dementia (PLWD).

Background: Hospitalized PLWD often experience unrelieved pain. Pain is common in PLWD. Undermanaged pain accelerates changes in cognition, memory loss, and functional decline. Nurses’ personal feelings and professional experiences with pain may lead to inaccurate knowledge and beliefs based on negative stereotypes of aging and dementia.

Methods: A mixed method study was conducted using an explanatory sequential design. The quantitative cross-sectional survey study measured nurses’ self-perceived knowledge and beliefs about pain assessment and management in PLWD. The …

Motivation In Community Engaged Learning: For Grades Or Growth?, Anastasios Catanzaro, Sarah Mclean

Journal of Community Engagement and Higher Education

Community-engaged learning (CEL) is a pedagogy characterized by reciprocal partnerships between academic institutions and community partners. Literature suggests that CEL has a potent ability to enhance skill, empathy and engagement in both students and community members. This investigation is a mixed methods study aimed to assess whether CEL can influence student motivation in a biomedical science CEL course. We found that student motivation is dynamic, and CEL supports both intrinsic and extrinsic motivation approaches.

The Impact And Strategies Utilized By College Students During The Covid-19 Pandemic, Kristi Lewis, Olivia Hansen, Amanda Kavaja

Journal of Community Engagement and Higher Education

The COVID-19 pandemic only exacerbated the growing mental health concerns seen on college campuses. A qualitative study was conducted to identify the impact and the strategies utilized by undergraduates during the pandemic. Semi-structured one-on-one interviews were conducted with 15 undergraduates. Findings suggest that college students encountered academic challenges and social struggles during COVID. The academic challenges and social struggles experienced during the pandemic have only added to the growing mental health concerns of college students.

Fish Oil And Vernonia Amygdalina Leaves-Supplemented Biscuits Increased Insulin Secretion And Suppressed Pro-Inflammatory Cytokines In Type 2 Diabetic Wistar Rats, Omolola Soji-Omoniwa, Uthman Olanrewaju Abdulazeez, Christfavour Oluwaseyi Oloba, Samuel Olajuwon Kolawole, Johnson Opeyemi Olayinka, Emmanuel Olorunleke Oludipe, Anuoluwapo Faith Ajediti, Habib Tajudeen Yusuf

Al-Bahir

This research determined the effect of fish oil and Vernonia amygdalina-enriched biscuits (FVSB) on insulin sensitivity and inflammatory cytokines in type 2 diabetic rats. Forty-nine male rats were grouped into seven, i.e. A-G. Type 2 diabetes (T2DM) was induced in groups B-G by administering 230 mg/kg body weight [BW] NAD (ip) and 65 mg/kg BW streptozotocin (ip). Group A represented the control (Non-diabetic + unsupplemented biscuit), B (untreated diabetic), C (diabetic + 120 mg/kg BW metformin), D-F (diabetic + FVSB at 1g, 2g and 3g leaf inclusion respectively), and G (diabetic + conventional biscuit). The experiment lasted 35 days, …

A Case Of Extra-Arterial Vascular Anomalies In A Patient With Williams Syndrome, Meredith Barrientez, Katherine Forrester, Margaret Rozier-Chen

Journal of Pediatric Genetics

Williams-Beuren syndrome (WBS), commonly abbreviated to Williams syndrome, is well described in the literature regarding its typical clinical presentation, which is the result of a heterozygous deletion within the genetic sequence located on chromosome 7q11.23. The gene for elastin (ELN) is located within this DNA sequence. Patients with WBS exhibit multi-organ involvement including cardiac abnormalities such as supravalvular aortic stenosis and connective tissue disorders due to the disruption of ELN.1 In this case, we present an infant born with extensive infantile hemangiomas, including cutaneous and organ involvement, verified by skin biopsy, and abnormal hepatic vessels. Our patient received an extensive …

Diagnosis And Initial Tratment Of Gorham-Stout’S Disease, Jesús González Cayón, Maria Del Carmen San Basilio Berenguer, Julio César Moreno Alfonso, Santiago De La Puente Pérez, Paloma Triana Junco, Juan Carlos López Gutiérrez

Journal of Pediatric Genetics

The Gorham-Stout’s disease, also known as massive osteolysis or evanescent bone disease; is an extremely rare disease characterized by a proliferation of intraosseous lymphatic channels causing progressive osteolysis. Usually, surgery, sclerotherapy and radiotherapy have been reserved for local and symptomatic control of the disease, while medical treatments have presented variable results that are not entirely satisfactory according to literature. The recent identification of germline-activating somatic mutations in the PIK3CA gene has opened the way to treatment with mTOR inhibitors, being Sirolimus an effective option with a low incidence of serious adverse effects. We present a patient diagnosed with GSD and …

Phenotyping And Provisional Diagnosis Of Mucopolysaccharidoses Based On Machine Learning, Srilatha Kadali, Shaik Mohammad Naushad, Vijaya Lakshmi Bodiga

Journal of Pediatric Genetics

In view of significant overlapping clinical features in mucopolysaccharidoses (MPS) subtypes, clinicians face difficulty in differential diagnosis, thus requiring the need for a machine learning-based clinical tool for the provisional diagnosis of MPS subtypes. Out of 520 patients with suspicion of MPS, 296 patients were identified with MPS types. To develop the model, we took 53 clinical symptoms of MPS patients (n=255) into account for differential diagnosis. The diagnosis was based on enzyme testing. Among mucopolysaccharidoses, MPS I was the most prevalent. Different machine learning tools were examined, and classification and regression tree (CART) emerged as the most promising. The …

Systematic Exploration Of Trpv6 Variants In Pancreatitis: Unraveling Genetic Associations And Pathophysiological Insights Through Comprehensive Systematic Review And In-Silico Analysis, Omar Alomari, Rumeysa Yegin, Muhammed Edib Mokresh, Ghazaleh Kokabi Ghahremanpour, Nurullah Komurcu, Elif Nur Ari, Magda Wojtara, Ali Karaman, Abdulqadir J. Nashwan

Journal of Pediatric Genetics

Background and Aims: The transient receptor potential vanilloid subfamily member 6 (TRPV6), a calcium-selective channel, is crucial for intracellular calcium (Ca2+) transport, impacting the maintenance of Ca2+ homeostasis in the pancreas. Variants in the TRPV6 gene have been associated with disruptions in calcium balance, potentially contributing to chronic pancreatitis (CP). This systematic review aims to unravel the connections between TRPV6 gene variants and pancreatitis, with a particular focus on CP. Additionally, we conducted an in-silico co-expression analysis to explore the interrelationships among validated genetic variants associated with pancreatitis pathophysiology and TRPV6 gene variants . Methods: A comprehensive literature search was …

Bard College: An Ecosystem Of Engagement, Jonathan Becker

Journal of Community Engagement and Higher Education

Despite its moderate size and rural location, Bard’s civic engagement efforts resonate locally, nationally, and internationally, and have significant public policy impacts. Bard has achieved success by making engagement central to its institutional mission, viewing liberal arts and sci-ences education as both a means and an end of civic engagement efforts, and forging an “ecosystem of engagement” that encourages organizational engineers, links student-led and in-stitutional initiatives, and unites a network of partners across the globe.

Healing On The Homefront: Convelescent Homes In England During Wwi, Elizabeth Rogerson

Montview Journal of Research & Scholarship

This paper explores the response to soldiers needing rehabilitation in World War One and how convalescent facilities impacted the everyday lives of injured men, nurses, and military medicine. During the Great War, there was an influx of the diversity of injuries due to the expanse of biological and machine warfare. Recognizing that field hospitals were insufficient, England’s War Office executed a plan to requisition public facilities and use volunteer forces not only to heal English men but soldiers from across the world. This paper establishes the context for the convalescing system in England and then its accommodation and improvisation for …

Antioxidants And Metabolic Biomarkers: Clinical Insights In Breast Cancer Patients, Rania Kareem, Emad Yousif, Taghried Salman, Muna Bufaroosha

AUIQ Technical Engineering Science

Breast cancer is the primary cause of cancer-related deaths among women and the most common life-threatening malignancy identified in this population. The second most common cause of cancer-related fatalities in women is breast cancer. According to the most recent Iraq Cancer Requisition, breast cancer is the most common kind of cancer in women in Iraq, making up almost one-third of all female malignancies. Total of 100 patients with breast cancer was used in this study and compared to 100 healthy groups. Serum levels of vitamin C, vitamin E, Triglycerides, HDL, prolactin, cysteine protease, glutathione peroxidase and glutathione reductase were measured …

An Online Scientific Twitter World: Social Network Analysis Of #Sciencetwitter, #Scicomm, And #Academictwitter, Man Zhang, Lisa Lundgren, Ha Nguyen

Instructional Technology and Learning Sciences Student Research

Understanding who makes up online affinity spaces as well as how information flows within those spaces is important as more people access news, research topics, collaborate with others, and entertain themselves. During a month-long period in summer 2021, we collected 100,000 tweets from 53,311 Twitter users who used the hashtags #ScienceTwitter, #SciComm, and #AcademicTwitter. We then classified users and determined the type of social network they formed. Scientists, the public, and educators formed this affinity space. They built connections by initiating activities and interacting with others, which created a Community Clusters social network structure, characterized by several medium-sized groups of …

Molecular Biophysics And Engineering Lab, University Of Mississippi. School Of Engineering

University Website Migration from WordPress

Molecular Biophysics sits at the crossroads of biology, chemistry, physics, and engineering—and is rapidly transforming how we understand life. Our lab focuses on the cytoskeletal machinery of the cell, aiming to uncover how molecular components work together to drive essential cellular processes. We study how motor proteins, crosslinkers, and cytoskeletal filaments coordinate to perform complex tasks like cell division, movement, and muscle contraction. By combining cutting-edge biophysical tools—including optical trapping, fluorescence microscopy, and QCM-D—we explore how these molecular interactions scale up to support the functions of living systems. This foundational knowledge helps reveal the fundamental design principles that govern living …

Multiscale Modelling Of Eg5: Structural Insights Into Microtubule Interactions, Atp Hydrolysis, And Allosteric Inhibition Mechanisms, Soundarya Priya Alexandar Ms

Theses and Dissertations

The mitotic kinesin EG5 is essential for spindle bipolarity and accurate chromosome segregation during cell division, making it a validated anti-cancer target. Unlike conventional microtubule-targeting agents, EG5 inhibitors offer mitosis-specific action, reducing neurotoxicity. Although inhibitors targeting EG5’s allosteric site-I have been extensively studied, mechanistic insights into inhibition at site-II, and their combined effects, remain underexplored. This thesis systematically investigates the structural and dynamic mechanisms underlying EG5 function and its inhibition using an integrative computational approach.

Conformational analysis, coarse-grained dynamics, all-atom molecular dynamics simulations, and protein network analysis were performed on EG5 motor domain structures and EG5-microtubule complexes. Results show that …

Monitoring The Hematologic Markers In Patients Undergoing Single-Stage Exchange Arthroplasty For Periprosthetic Joint Infection, Wenbo Mu, Juan D. Lizcano, Boyong Xu, Wentao Guo, Abudousaimi Aimaiti, Xiaogang Zhang, Javad Parvizi, Li Cao

Rothman Institute Papers

BACKGROUND: Periprosthetic joint infection (PJI) is a serious complication that necessitates a complex treatment strategy. Single-stage exchange arthroplasty, combined with intravenous and intra-articular antibiotic infusions, has shown high efficacy in treating complex PJIs. However, the impact of this approach on hematologic parameters remains underexplored. This study aims to evaluate the postoperative trends in blood platelet count, white blood cell (WBC) count, and neutrophil count in patients undergoing single-stage exchange arthroplasty.

METHODS: A retrospective analysis was conducted on 313 patients who underwent single-stage revision for PJI between June 2010 and October 2022. Hematologic parameters were monitored for the first seven postoperative …

Nrf2 Drives Activation-Driven Expansion Of Cd4+T Cells By Modulating Glucose And Glutamine Metabolism, Aprajita Tripathi, Debolina Dasgupta, Michael S. Dahabieh, Rachel Griffard-Smith, Anil Pant, Ashlyn Bugbee, Nanda Kumar Yellapu, Emily Burt, Ben H Y Choi, Abigail E. Ellis, Ryan D. Sheldon, Shailendra Giri, Devin Koestler, Russell G. Jones, Viveka Nand Yadav, Kalyani Pyaram

Manuscripts, Articles, Book Chapters and Other Papers

Upon antigenic stimulation, CD4+T cells undergo clonal expansion elevating their bioenergetic demands and utilization of nutrients like glucose and glutamine. The nuclear factor erythroid-2-related factor 2 (Nrf2) is a well-known regulator of oxidative stress, but its involvement in modulating the metabolism of CD4+T cells remains unexplored. We report that Nrf2 protein levels are temporally regulated in activated CD4+T cells, with elevated expression during early activation followed by a decline. T cell-specific constitutive activation of Nrf2, by deletion of its negative regulator Keap1, enhances early activation and interleukin-2 (IL-2) expression, upregulates T cell receptor (TCR) signaling, and increases activation-driven expansion of …

Practice Patterns In Management Of Low- To Intermediate-Grade Salivary Gland Carcinoma: A Multi-Institutional Study, Mirabelle Sajisevi, Kenny Nguyen, Peter Callas, Andrew Holcomb, Emre Vural, Kyle Davis, Carissa Thomas, John Stein, Ameya Asarkar, Ricardo Aulet, Rebecca Bell, Michael Blasco, Vanessa Bowmaster, Clayton Burruss, Jeffson Chung, Kimberly Chan, Brent Chang, Charles Coffey, David Cognetti, Dylan Cooper, Joehassin Cordero, John Donovan, Yue Jennifer Du, Yusuf Dundar, Rogerio Dedivitis, Heather Edwards, Boban Erovic, Antoine Eskander, Philip Feinberg, Emily Garvey, David Goldstein, Joseph Goodman, Rafael Goulart, Neerav Goyal, Stefan Grasl, Jonathan Giurintano, Nikita Gupta, Andy Habib, Trevor Hackman, Jared Hara, Christina Henson, Michael Hinni, Nadia Hua, Stephanie Johnson-Obaseki, Aditya Juloori, Noah Kalman, Alexandra Kejner, Sobia Khaja, Jamie Ku, Arnaud Lambert, Bao Luu, Kelly Magliocca, Luiz Medina Dos Santos, Cara Michael, Brett Miles, Giulianno De Melo, Michael Moore, Gregoire Morand, Kauê Moura, Hesameddin Noroozi, Rusha Patel, Joseph Paydarfar, Karolina Plonowska-Hirschfeld, Nader Sadeghi, Fabrice Savaria, Nicole Schmitt, Justin Shapiro, Timothy Shaver, Sandro Stoeckli, William Stokes, Anita Sulibhavi, Jason Tasoulas, Varun Vendra, Daniel Vinh, Celina Virgen, Neil Woody, Geoffrey Young, Kiran Kakarala, Danny Enepekides, Michael Hier, Louise Davies, William Ryan

Department of Otolaryngology - Head and Neck Surgery Faculty Papers

OBJECTIVES: To characterize practice patterns and outcomes in the management of low- and intermediate-grade salivary gland carcinoma prior to the existence of treatment guidelines.

METHODS: Analysis of a registry of patients who underwent parotid and submandibular gland resections for low-and intermediate-grade carcinomas between 2010 and 2019.

RESULTS: Of all 786 patients included in the study, 726 (92%) had preoperative imaging and 653 (83%) had preoperative biopsy. Of the 729 patients with parotid gland cancer, the majority underwent superficial (n = 384, 53%) or total (n = 254, 35%) parotidectomy. In patients with facial nerve preservation, total parotidectomy was …

Mt1-Mmp Inhibition Rejuvenates Ageing Brain And Rescues Cognitive Deficits In Obesity, Pallavi Asthana, Liguo Li, Lin Lu, Jiayan Wu, Shuo Zhang, Ningning Li, Sheung Kin Ken Wong, Susma Gurung, Yijing Zhang, Yuwan Lin, Yufeng Peng, Zongtang Xu, Kui Ming Chan, Lixiang Zhai, Aiping Lyu, Zhao-Xiang Bian, Xin Ge, Ashok Iyaswamy, Min Li, Ya Su, Zhongjun Zhou, Pingyi Xu, Hoi Leong Xavier Wong

The Brown Foundation: Institute of Molecular Medicine

Obesity has been linked to an increased risk of cognitive impairment and dementia in later life. Although aging and obesity are both associated with cognitive decline, it remains unclear how they interact to affect cognitive function across the lifespan and how brain function might mediate their relationship with cognition. Our previous findings and other studies have shown that membrane type 1-matrix metalloproteinase (MT1-MMP/MMP14), which increases with age, regulates energy homeostasis. Inhibiting MT1-MMP improves insulin sensitivity, reduces body fat, and lowers serum cholesterol. Here, we demonstrate that MT1-MMP links neuroinflammation to cognitive decline in aging and obesity. Inflammatory responses in the …