Digital Commons Network^™

An Analysis Of Diagnostic Metabolomic Profiles Associated With Hepatotoxicity During Childhood All Induction Therapy, Emily J Mason, Anna M Crain, Michael E Scheurer, Philip J Lupo, Karen R Rabin, Olga A Taylor, Marley Roberts, John P Woodhouse, Ashley Chavana, Kathleen Ludwig, Laura Klesse, Kenneth Heym, Timothy Griffin, Rodrigo Erana, Juan Carlos Bernini, M Monica Gramatges, Joanna S Yi, Sandi L Pruitt, M Brooke Bernhardt, Hong Zhu, Steven D Mittelman, Van Huynh, Etan Orgel, Jeremy M Schraw, Austin L Brown

Faculty, Staff and Students Publications

Hepatotoxicity is a well-documented complication of induction chemotherapy for acute lymphoblastic leukemia (ALL), but our understanding of its biological mechanisms is limited. We identified 314 patients with ALL (aged 1-19 years) treated at Texas Children’s Hospital (2008-2019) with diagnostic bone marrow plasma available for metabolomic profiling: 234 for discovery and 80 for replication. Hepatotoxicity during induction was defined as follows: (1) transaminitis: grade ≥3 aspartate aminotransferase or alanine aminotransferase or (2) conjugated hyperbilirubinemia: conjugated bilirubin (c.bili) >3 mg/dL. Untargeted profiling detected 519 metabolites. Adjusted odds ratios (aORs) for each metabolite were calculated with logistic regression, accounting for sex, age, body …

Turning Patients' Open-Ended Narratives Of Chronic Pain Into Quantitative Measures: Natural Language Processing Study, Raquel Norel, Jennifer Gewandter, Zhengwu Zhang, Anika Tahsin, Chadi G Abdallah, John Markman, Zhiyao Duan, Guillermo Cecchi, Paul Geha

Faculty, Staff and Students Publications

Background: Subjective report of pain remains the gold standard for assessing symptoms in patients with chronic pain and their response to analgesics. This subjectivity underscores the importance of understanding patients' personal narratives, as they offer an accurate representation of the illness experience.

Objective: In this pilot study involving 20 patients with chronic low back pain (CLBP), we applied emerging tools from natural language processing (NLP) to derive quantitative measures that captured patients' pain narratives.

Methods: Patients' narratives were collected during recorded semistructured interviews in which they spoke about their lives in general and their experiences with CLBP. Given that NLP …

Hemolysis Detection Using The Gem 7000 At The Point Of Care In A Pediatric Hospital Setting: Does It Affect Outcomes?, Ridwan B Ibrahim, Nazmin Bithi, Charlene Hernandez, Hanna Uhrova, Brandy D Recio, Sridevi Devaraj

Faculty, Staff and Students Publications

No abstract provided.

Nivolumab Plus Ipilimumab Induce Hyper-Progression In Renal Medullary Carcinoma: Results Of A Phase Ii Trial And Preclinical Evidence, Melinda Soeung, Xinmiao Yan, Ciro Zanca, Jing Qian, Menuka Karki, Fei Duan, Hania Khan, Li Zhang, David H Peng, Mariah Williams, Rong He, Ziheng Chen, Luigi Perelli, Jianfeng Chen, Rebecca S Tidwell, Pankaj K Chauhan, Courtney N Le, Truong N A Lam, Nirjar Bhattacharya, Rutvi Shah, I-Lin Ho, Jason P Gay, Caroline C Carrillo, Ningping Feng, Kang Le, Guang Gao, Teresa L Perry, Faika Mseeh, Yongying Jiang, Quanyun A Xu, Niki Marie Zacharias, Rahul A Sheth, Tharakeswara K Bathala, Priya Rao, Najat C Daw, Durga N Tripathi, Cheryl L Walker, Mohammad M Mohammad, Jianhua Zhang, Guangchun Han, Yanshuo Chu, Ruiping Wang, Minghao Dang, Enyu Dai, Fuduan Peng, Yunhe Liu, Akshaya Jadhav, Wenhua Lang, Claudio A Arrechedera, Leticia Campos Clemente, Edwin R Parra, Hsinyi Lu, Cara L Haymaker, Ignacio I Wistuba, Andrew Futreal, Andrea Viale, Michael J Soth, Philip Jones, Joseph R Marszalek, Timothy Heffernan, Giulio F Draetta, Nizar M Tannir, Jianjun Gao, Linghua Wang, Giannicola Genovese, Pavlos Msaouel

Faculty, Staff and Students Publications

Therapeutic options for patients with renal medullary carcinoma (RMC) are limited. Here we report the results of a phase II clinical trial (NCT03274258) of anti-PD1 nivolumab plus anti-CTLA4 ipilimumab in patients with RMC, with objective response rate as primary outcome. Enrollment was halted for futility at a prespecified interim analysis as all 10 treated patients experienced rapid disease progression. 5/10 met radiological criteria for hyperprogression and median progression-free survival (secondary outcome) was 1.38 months (95% confidence interval: 1.28, 1.60). In a post-hoc single-cell RNA sequencing analysis, data from patients with RMC before and after nivolumab plus ipilimumab treatment indicated that …

Leaping Between Branches: Hybridisation And The Tangled Evolutionary History Of True Lemurs, Giacomo Mercuri, Giovanni Merici, Kyle Kai-How Farh, Lukas F K Kuderna, Jeffrey Rogers, Tomàs Marques-Bonet, Giuseppe Donati, Riccardo Percudani, Cristian Capelli

Faculty, Staff and Students Publications

The true lemurs (genus Eulemur) are a genetically diverse and spatially widespread group of species inhabiting most of Madagascar's forests. Including 12 recognized species, the genus can be divided into four major evolutionary groups: E. rubriventer, E. mongoz, the Brown Lemur Species Complex (BLSC), and the coronatus-macaco-flavifrons complex (CMFC), although monophyly for the CMFC is not always supported. Recent genome-based studies highlighted topological and chronological differences between nuclear and mitochondrial phylogenies of true lemurs, which could be explained by events of hybridisation. In order to reconstruct the evolutionary history of the genus, we test for gene-flow between Eulemur clades using …

Deep Learning Reveals How Cells Pull, Buckle, And Navigate Fibrous Environments, Abinash Padhi, Arka Daw, Atharva Agashe, Medha Sawhney, Maahi M Talukder, Mehran M H Pour, Mohammad Jafari, Guy M Genin, Farid Alisafaei, Sohan Kale, Anuj Karpatne, Amrinder S Nain

2020-Current year OA Pubs

Cells in tissues navigate fibrous environments fundamentally differently than they do on flat substrates, but the establishment of cell forces in physiological fibrous settings remains poorly understood. Although factors such as the stiffness of the extracellular matrix (ECM) are known to drive behaviors, including cell motility on flat nonfibrous substrates, the interplay between fiber architecture and stiffness in fibrous ECM is not known. Here, we find that in fibrous environments, the directionality of mechanical forces overrides ECM stiffness as the primary regulator of contractility in migrating cells. Using an approach combining phase microscopy with deep learning to map forces in …

Mitochondrial Iron Transport Via Mfrn1 Is Required For Erythroid Cell Cycle Progression, Mark Perfetto, Aidan Danoff, Muhammad Ishfaq, Heidi Monroe, Aiden Mohideen, Meilin Chen, Jesus Tejero, Amber N Stratman, Satoshi Okawa, Yvette Y Yien

2020-Current year OA Pubs

Iron metabolism drives key erythropoietic processes, including hemoglobinization, survival, and proliferation. Here, we developed in vivo methods to interrogate how iron regulates erythropoiesis and report that mitochondrial iron transport via mitoferrin-1 (MFRN1) is essential for erythroid cell cycle progression. mfrn1 embryos had severely decreased erythroid cell number caused by cell cycle arrest at G2/M. They had enlarged nuclei, suggesting a mitotic defect. Iron supplementation rescued the cell cycle defect, implicating mitochondrial iron deficiency as its cause. In contrast, fpn1 mutants, anemic from systemic iron deficiency, had less severe decreases in erythroid mitochondrial iron than mfrn1 mutants and no proliferative defects. …

Bio-Corrosion Studies On Novel Zr‑Co‑Ti Based Metallic Glass Alloys For Biomedical Implant Applications, Shubhra Shitole

Thesis/ Dissertation Defenses

The relentless pursuit of advanced biomaterials that synergistically combine high mechanical strength, exceptional corrosion resistance, and inherent biocompatibility is critical for next-generation medical implants. This research addresses this challenge through the development and multi-faceted evaluation of a novel library of Zr-Co-Ti-based metallic glasses (MGs); Zr₆₀Co₃₀Ti₁₀, Zr₅₅Co₃₅Ti₁₀, and Zr₅₀Co₄₀Ti₁₀, fabricated via melt-spinning. The alloys were confirmed to be fully amorphous by X-ray diffraction (XRD), a structure underpinned by exceptional thermal stability, with the Zr60 composition exhibiting a larger supercooled liquid region (Δ𝑇𝑥) of …

Determination Of Steroid Hormones And ⍺-Zeranol In Beef Products In East Tennessee, W. Colby Huffman, S. Ellie Jaynes, Nathan C. Duncan, D. Andrew Crain

RURALS: Review of Undergraduate Research in Agricultural and Life Sciences

Hormone growth implants are commonly used in cattle to increase growth and there is some concern that these implants may result in altered hormone concentrations in the beef from implanted cattle. This study aims to (1) develop a simple, standard, cost-effective method to measure hormones in beef and (2) compare hormones in meat from cattle not exposed to growth implants (negative controls), cattle exposed to growth implants (positive controls), and from a commercial retail store. Extraction and measurement using LCMS/MS was successful in detecting 17b-estradiol, progesterone, testosterone, and a-zeranol in meat, with a trend to lower concentrations in meat not …

Immunological Surveillance Against Cancer Across Mammals, Orsolya Vincze, Piotr Minias, Alexandre Corthay, Fernando Colchero, Jean-François Lemaître, Louise Maille, Tamás Malkócs, Justus Hagemann, Dalia A. Conde, Samuel Pavard, Antoine M. Dujon, Beata Ujvari, Frédéric Thomas, Amy M. Boddy, Carlo C. Maley, Damien Chevallier, Tuul Sepp, Thomas Pradeu, Mathieu Giraudeau

Presidential Fellows Articles and Research

Contrary to expectations based on their higher cell numbers, larger and longer-lived species do not face dramatically increased risk of cancer. This strongly suggests that evolution has fashioned natural cancer resistance mechanisms, yet our knowledge remains limited on what these mechanisms might be. The cancer immunological surveillance hypothesis, proposed by Burnet and Thomas in the 1950s, highlights immunity as a key factor determining species-specific cancer resistance. Here we address the original, evolutionary interpretation of this hypothesis by investigating the relationship between cancer mortality risk and markers of efficient antigen presentation. Our results show that the expansion of the MHC class …

Dissecting The Efficacy And Immunogenicity Of Tlr7 Agonist–Antibody Conjugates Through The Lens Of Fc Effector Function, Conjugation Strategies, And Linker Cleavability, Siteng Fang, Samantha R. Benjamin, Lina Wu, Mohammad Asikur Rahman, Kayla K. Elder, Victor T. Ojo, Tao Zhang, Tracy A. Brooks, L. Nathan Tumey

Pharmacy Faculty Scholarship

The advent of immuno-oncology therapeutics has given rise to antibody-drug conjugates (ADC) designed to selectively activate pattern recognition receptors such as Toll-Like Receptors (TLRs). In contrast to classical ADC technology, these Immune Stimulating Antibody Conjugates (ISACs) are widely reported to rely on Fcγ-mediated uptake and noncleavable linkers. Herein, we systematically study the impact of Fc effector function, linker cleavability, and conjugation strategy on the efficacy and immunogenicity of a series of TLR7 agonist-based ISACs. We demonstrate that the combination of FcγR-ablation and incorporation of a cleavable linker results in increased stimulation of myeloid cells, enhanced efficacy, and improved tolerability as …

A Silver Spoon Effect Reduces Lifetime Fitness In A Declining Loon Population, Walter H. Piper, Claudia Kodsuntie, Hayden Walkush

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

A complete understanding of factors that influence animal fitness requires that we measure not only those occurring day to day in the life of an animal but also those that operate on longer time scales. Here, we investigated silver spoon effects (fitness impacts resulting from conditions faced early in life) and carryover effects (fitness impacts caused by environmental factors in a previous season) in a northern Wisconsin population of the common loon (Gavia immer). The mass of a loon chick divided by its age, an indication of food it received from its parents in its first 4 to …

The Effect Of Noise Pollution On The Diversity And Abundance Of Bird Species At Different Locations At Warner Parks, Nashville, Tn, Jeremy T. Little, Wyatt L. Fooks, Darlene Panvini, Jackson Whiteside

Science University Research Symposium (SURS)

Noise pollution is a significant abiotic factor that can alter bird biodiversity and abundance of birds in urbanized areas. A new road is proposed through a relatively untouched area of Warner Parks in Nashville, TN, which may affect the bird community. Acoustic Song Meters were used to monitor bird sounds and soundscapes in the proposed road site and two areas of the park with different levels of noise pollution (roadside=high noise; isolated=low noise) for three weeks. Recordings were analyzed to determine bird species and abundance at each site to predict how increasing noise pollution may affect bird diversity in the …

Antifungal Effects Of Fatty Acids On The Growth Of Ophidiomyces Ophiodiicola In A Simulated Snake Skin Environment, Krupa S. Patel, Lizbeth Delarosa, Jacey Cardenas, Thalia Rodriguez, John Niedzwiecki

Science University Research Symposium (SURS)

Emerging infectious diseases have increasingly threatened wildlife populations worldwide, with devastating impacts observed in frogs, bats, and more recently, snakes. Snake fungal disease (SFD), caused by the pathogenic fungus Ophidiomyces ophiodiicola (Oo), currently poses a serious threat to snake populations across multiple regions. Infected snakes exhibit disrupted skin shedding, rough or scaly patches, crusty sores, and ulcers that compromise their health and survival. Although the mechanisms underlying transmission and susceptibility to SFD remain unclear, the snake cutaneous microbiome is known to act as a defensive barrier, mediating interactions between the host and invading pathogens. The lipid layer is a key …

Fungal Foes And Fatty Defenses: How Simulated Snake Skin Lipids Influence Ophidiomyces Ophiodiicola Growth, Thalia Rodriguez, Jacey Cardenas, Lizbeth Delarosa, Krupa Patel, John Niedzwiecki

Science University Research Symposium (SURS)

Emerging fungal diseases are a continuous threat to wildlife health as well as biodiversity. Fungal diseases, such as white nose syndrome in bats, can devastate populations by disrupting their ecosystems. Many other species around the world are affected by fungal diseases, such as amphibians, birds, and snakes. In more recent years, snake fungal disease (SFD) emerged due to the fungal pathogen Ophidiomyces ophiodiicola (O.o). Like Pseudogymnoascus destructans, the fungal pathogen responsible for white nose syndrome, O.o targets the skin which contains many fatty acids. Some studies have shown that fatty acids present in bat skin are able to inhibit fungal …

Surviving The Seasons: Climate And Time Effects On Native Songbirds, Essence Donaldson, Todd Hoskinson, John Niedzwiecki

Science University Research Symposium (SURS)

Bird populations across North America have declined sharply in recent decades due to habitat loss, climate change, and changes in food availability. As bird numbers fall, ecosystems lose essential pollinators, seed dispersers, and insect predators. Long-term monitoring of bird populations is vital for understanding how environmental changes influence their survival and abundance. This study examines capture data from a long-term bird banding station at Percy Warner Nature Center in Nashville, Tennessee, focusing on five native species: Northern Cardinal (NOCA), American Robin (AMRO), American Goldfinch (AMGO), Tufted Titmouse (TUTI), and Carolina Chickadee (CACH), from fall 1983 to fall 2023. We analyzed …

Kairiomone Moderated Anti-Predator Responses In Pleurocera Laqueta, Daisy V. Mcleod, John Webb, Amanda Waggoner, John Niedzwiecki

Science University Research Symposium (SURS)

Predator-prey coevolution in freshwater ecosystems often involves chemical communication, where the prey detect predator released compounds known as kairomones. These chemical cues allow prey to alter behavior to reduce risk of predation. In Middle Tennessee streams, the native snail Pleurocera laqueata commonly coexists with predator crayfish, and previous studies have shown that exposure to crayfish kairomones can induce an anti-predator “crawl out” response. This response is commonly demonstrated through snails climbing above the waterline to avoid crayfish predators below. We hypothesized that Pleurocera laqueata would display a similar anti-predator response when exposed to both native and non-native crayfish cues. After …

Physiological Response To Concealed Information In A Mock-Crime Paradigm​, Ezra Koenigsberg, Tamya Mchenry, Jasmine Manzana, Taylor Powell, Samantha Jackson

Science University Research Symposium (SURS)

The Concealed Information Test (CIT), based on Lykken’s guilty knowledge framework, measures physiological responses when individuals recognize crime-related details compared to neutral alternatives. Previous studies show that variations in stimulus presentation enhance the CIT’s accuracy, especially through changes in electrodermal activity and heart rate. However, few studies have tested whether a Qualtrics-based mock-crime version of the CIT can replicate these physiological patterns. This study addresses that gap by adapting the CIT for a digital research environment. The goal is to determine whether participants show greater physiological arousal—measured through skin conductance, heart rate, and respiration—when exposed to crime-related “critical” stimuli compared …

Deconstructing The Black Box: An Explainability Analysis Of Deep Learning Architectures In Cytopathology, Chase A. Garrett

Master's Theses or Doctor of Nursing Practice

Deep learning shows strong potential in medical-image analysis, yet adoption in cyptopathology remains limited. Cytopathology could benefit from deep learning applications by improving diagnostic efficiency and accuracy. However deep learning comes with a notorious “black box” that keeps the models from being transparent and trustworthy for widespread clinical adoption. We conducted a comprehensive and comparative analysis of several deep learning architectures for multi-class classification of acute leukemia types, ALL, AML, and normal healthy cells from peripheral blood smear images. The models in this research include a Vision Transformer (ViT) and a diverse selection of Convolutional Neural Network (CNN) models. The …

Characterization Of Antiretroviral Therapy (Art) Adherence Phenotypes And Psychosocial Symptom Clusters Among Black/African American (Aa) And Hispanic/Latine Adolescents And Young Adults (Ayas) With Hiv In The Adherence Connection For Counseling, Education, And Support (Access-Ii) Trial, Ann-Margaret Navarra, Taehoon Ha, Eva Liang, Maurade Gormley, David R. Garcia, Jason Fletcher, Lloyd A. Goldsamt, Michael G. Rosenberg, Karin Hasegawa, Jie Yang

School of Nursing Faculty Publications

Antiretroviral therapy (ART) adherence behavior is heterogeneous among adolescents and young adults (AYAs) with HIV and influenced by individual and interpersonal psychosocial factors. The primary objective of this study is to characterize ART adherence phenotypes and psychosocial symptom clusters, as related to ART adherence and HIV viral load suppression. This analysis included 60 AYAs with HIV enrolled in an ART adherence support clinical trial. Self-reported ART adherence at baseline, 12-weeks, and 24-weeks was used to define four ART adherence phenotypes: consistently high adherence (YY), early-only adherence (YN), late-only adherence (NY), and consistently low adherence (NN). Symptom clusters were empirically derived …

Some Like It Hot -Structural Changes In Extremophile Rubredoxin At 120 °C., Tzanko Doukov, Igor Leontyev, Francis E. Jenney, Dominic George, Stephen P Cramer

PCOM Scholarly Works

How does the structure of a protein change as the temperature is raised from cryogenic conditions at 100 K to 393 K? Understanding the structure and dynamics of proteins under environmental extremes is relevant for human health, biotechnological applications, and our search for life elsewhere in the universe. Here we reveal the high temperature crystal structure of a hyperthermophilic (Pyrococcus furiosus) rubredoxin at 393 K (120 °C), together with multiple complementary structures down to 100 K. The results are compared with molecular dynamics calculations. Significant changes in H-bonding are observed. Discussions about high-temperature protein structure and stability need to recognize …

A Geothermal Amoeba Sets A New Upper Temperature Limit For Eukaryotes, H Beryl Rappaport, Natalie A. Petek-Seoane, Tomáš Tyml, Felix Mikus, Kurt Labutti, Godwin Ani, Jessica K. Niblo, Ethan Macvicar, Rachel M. Shepherd, Ignacio De La Higuera, Samuel J. Lord, Kenneth M. Stedman, Multiple Additional Authors

Center for Life in Extreme Environments Publications

The study of temperature limits has transformed our knowledge of the boundaries of life but has been largely focused on bacteria and archaea. We isolated a novel geothermal amoeba, , that divides at 63°C (145.4°F), establishing a new record for the upper temperature limit across all eukaryotes. We demonstrated cellular proliferation with growth experiments and visualized mitosis expansion microscopy. Using high-temperature live-cell imaging, we quantified movement up to 64°C. We assembled the genome of and using comparative genomics found an enrichment of genes related to proteostasis, genome stability, and sensing the external environment. Taken together, our findings challenge the current …

Navigating The Future: Healthcare Professionals’ Perspectives On Cobot Integration In Hospitals, Amanda Spence, Kellie Patterson, Stephanie Rogers, Paige Merring, Wendy Bassett, Stephanie Roth, Sanaz Taherzadeh, Susan Smith Birkhoff, Briana Abernathy, Hannah Rackie, Erin Mitchell, Morgan Tallo

Biennial Convention (CONV)

There is growing interest in the implementation of delivery robots in healthcare, yet a lack of understanding remains regarding frontline clinicians’ views on the implementation of these robots. Limited research exists addressing the perceptions, attitudes, and experiences of staff following the integration of delivery robots into clinical practice. Gaining insight into staff perspectives is essential for the effective integration and optimization of robots in healthcare settings.

This study aims to explore the perceptions of healthcare workers after the implementation of delivery robots within a large tertiary care hospital setting.

Guided by Bowden’s feasibility framework, a mixed methods design with a …

From Fairy Tales To Fascist Nightmares: Countering Ron Desantis’ Florida, Ronald Mize, Et. Al

University of Miami Race & Social Justice Law Review

The recent spate of anti–‘woke’, don’t say gay, anti–trans, and anti–immigrant legislation, led by Florida Governor Ron DeSantis, puts the sunshine state in the unenviable position of serving as the nation’s test case for Republicans’ neofascist agendas. This paper explores the exclusionary, targeting, and banning aspects of these culture war assaults on public education and outgroups. The specific banning of Critical Race Theory is discussed alongside cis–heteronormative efforts to target queer and trans communities, as well as DeSantis’ executive memoranda of transporting those at the border to sanctuary cities and S.B. 1718 aimed at excluding immigrant communities from public life …

Macrophyte Community Analysis Of The Tennessee-Tombigbee Waterway, The Pearl River, And The Pascagoula River, Samuel A. Schmid, Nicholas J. Engle-Wrye, Gray Turnage

Research Data

The macrophyte Tennessee-Tombigbee Waterway, Pearl River, and Pascagoula River were surveyed using point surveys. Data collected from these surveys were used to determine differences in species composition among macrophyte communities, how latitude affected species richness, and how the presence/absence of introduced species was affected by native richness. Differences in species composition were measured using NMDS, and the latitude-richness and introduced-native relationships were modeled using linear modeling methods. This record includes the data and code used for these analyses.

Single-Cell Genomics Reveals Complex Microbial And Viral Associations In Ciliates And Testate Amoebae, Frederik Schulz, Ying Yan, Agnes K. M. Weiner, Ragib Ahsan, Laura A. Katz, Tanja Woyke

Biological Sciences: Faculty Publications

Protists play important roles in nutrient cycling across ecosystems, yet the composition and function of their associated microbiomes remain poorly studied. Here, we use cultivation-independent single-cell isolation and genome-resolved metagenomics to investigate the microbiomes and viromes of more than 100 uncultivated ciliates and amoebae from diverse environments. Our findings reveal unique microbiome structures and complex associations with bacterial symbionts and viruses, with stark differences between ciliates and amoebae. We recover 117 microbial genomes affiliated with known eukaryotic endosymbionts, including Holosporales, Rickettsiales, Legionellales, Chlamydiae, and Babelota, and 258 genomes linked to host-associated Patescibacteriota. Many show …

Development And Applications Of A Health Equity Summary Score Using The Medical Expenditure Panel Survey, Ronald R. Sanchez

Dissertations and Theses

Despite sustained national efforts, disparities in healthcare delivery persist among racial and ethnic minorities, low-income individuals, and socially at-risk populations. Monitoring modifiable aspects of care delivery, such as the structures and processes that health systems can directly improve, is a key strategy for addressing these gaps. However, most measures evaluate modifiable aspects of care delivery separately, limiting understanding of how improvements in one domain reinforce the other and how their combined effect influences equitable care.

This dissertation addresses this gap by combining patient experience and clinical quality, two modifiable aspects of care that captures how patients interact with the health …

Red Snapper (Lutjanus Campechanus) Neurocranium, Gabriella K. Flora, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Assessment Of Traffic Related Air Pollution Effects On Indoor Air Quality In Educational Buildings, Ahmed Gouda Mohamed, Mohamed Sherif Dr., Fahad Alqahtani Dr., Yousif Deeb Eng.

Civil Engineering

This study assesses the impact of traffic-related air pollution (TRAP) on indoor air quality (IAQ) within a Riyadh school. The main objectives are to pinpoint factors influencing IAQ and to evaluate the effectiveness of existing ventilation and filtration systems. Air quality Egg sensors monitored nitrogen dioxide (NO2), carbon monoxide (CO), and particulate matter (PM1.0, PM2.5, PM10) levels both indoors and outdoors over 30 days. Findings revealed average indoor PM2.5 and PM10 concentrations of 14.09 µg/m3 and 18.15 µg/m3, respectively, while outdoor concentrations averaged 20.63 µg/m3 and 27.88 µg/m3. A strong positive correlation was observed between indoor and outdoor PM levels, …

High-Throughput Screens Identify Genotype-Specific Therapeutics For Channelopathies, Christian L. Egly, Alex Shen, Tri Q. Do, Carlos Tellet Cabiya, Paxton A. Ritschel, Suah Woo, Matthew Ku, Brian P. Delisle, Brett M. Kroncke, Björn C. Knollmann

Physiology Faculty Publications

Genetic diseases such as ion channelopathies substantially burden human health. Existing treatments are limited and not genotype specific. Here, we report a 2-step high-throughput approach to rapidly identify drug candidates for repurposing as genotype-specific therapy. We first screened 1,680 medicines using a thallium-flux trafficking assay against Kv11.1 gene variants causing long QT syndrome (LQTS), an ion channelopathy associated with fatal cardiac arrhythmia. We identified evacetrapib as a suitable drug candidate that improves membrane trafficking and activates channels. We then used deep mutational scanning to prospectively identify all Kv11.1 missense variants in an LQTS hotspot region responsive to treatment with evacetrapib. …