Digital Commons Network^™

Mitochondria-Mediated Epigenetic Transfer Between Chondrosarcoma And Wild-Type Cells, Caleb C. J. Wyckoff

Biomedical Sciences Theses & Dissertations

Glioblastoma (GB), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), cholangiocarcinoma, and chondrosarcoma (CS) cancers all contain mutations in the gene isocitrate dehydrogenase 2 (IDH2). The mutant IDH2 enzyme exhibits transformation of alpha-ketoglutarate (αKG) into the oncometabolite D-2-hydroxyglutarate (D2HG) in the mitochondria of these cancers. Mitochondrial-mediated transfer between cancer cells and recipient cells is a significant event that impacts the physiology of the receiving cell, specifically the epigenetic landscape. Previous experiments indicating increased DNA methylation in mesenchymal stem cells exposed to IDH1 or IDH2 conditioned medium underscore the potential role of D2HG to alter methylation states. Additionally, the presence of …

Assessing The Impact Of Maternal Oral Health On The Risk Of Adverse Birth Outcomes, May Amr Salama

Health Services Research Dissertations

Preterm birth (PTB) and low birth weight (LBW) are leading causes of neonatal morbidity and mortality. Maternal periodontal disease (PD) has been increasingly linked to these adverse birth outcomes (ABOs), yet questions about causality and treatment effectiveness persist due to study design heterogeneity and potential unmeasured confounding. These gaps hinder the development of clear clinical and public health guidelines. This dissertation addresses these gaps through three interconnected studies assessing the relationship between maternal oral health and ABOs.

The first study employed a meta-epidemiological analysis of 62 original studies from nine systematic reviews to evaluate the impact of unmeasured confounding on …

Understanding Executive Order 13583’S Impact To Federal Workforce Diversity & Employee Perception Of Federal Policies And Programs, Michael Robert Arenella

School of Public Service Theses & Dissertations

Creating a more diverse, equitable, and inclusive (DE&I) workforce produces beneficial organizational outcomes, such as decreased turnover, improved creativity and innovation, and improved organizational performance (Diaz-Garcia et al., 2013; Gomez & Bernet, 2019; McLeod et al., 1996; Singh & Selvarjan, 2013). While the private sector has placed much emphasis on improving DE&I in their hiring practices, whether the federal government has achieved comparable outcomes remains unclear. Guided by the punctuated equilibrium theory (PET), this dissertation examines the impact of Executive Order 13583 on both change in workforce composition, and employee sentiment towards diversity policies and programs.

Using a multi-level, multi-method, …

United States V. Skrmetti: L’Identité De Genre Dans Le Droit Et La Politique De L’Égale Protection, Olatunde C.A. Johnson

Faculty Scholarship

French Abstract: L’article analyse l’arrêt United States v. Skrmetti, dans lequel la Cour suprême américaine juge que l’interdiction, par le Tennessee, des bloqueurs de puberté pour les mineurs trans ne viole pas la clause constitutionnelle d’égalité, dite clause « d’égale protection ». Refusant de considérer qu’il s’agit d’une discrimination fondée sur le sexe, la Cour applique le contrôle le plus déférent (base rationnelle) et accepte l’argument de l’État selon lequel il protège les mineur·es de risques médicaux incertains. L’auteure montre ici que cette approche s’inscrit dans un contexte plus large de lois et de décrets fédéraux visant les personnes …

Existence, Difference, And The Nigerian Woman: A Feminist Praxis Beyond Patriarchy, Hannah Yemisi Ajewole

Dissertations

This dissertation investigates the postcolonial condition of Nigerian woman through a critical philosophical engagement with feminist theory, focusing particularly on Simone de Beauvoir’s existentialism and Irigaray’s ethics of sexual difference. It interrogates the historical processes through which gender became the basis of hierarchy in the Nigerian society, arguing that colonial and patriarchal override precolonial forms of relationality—marked by dual-sex relationship and age-based hierarchies—into rigid, male-dominated structures of authority and representation. Drawing also on Oyèrónkẹ́ Oyěwùmí’s critique of western gender epistemologies, the work contextualizes the Nigerian woman’s experience within a broader matrix of colonial imposition, cultural displacement, and epistemic violence. This …

Ukrainian Women In The Russo-Ukrainian War: Between Survival And Resistance, Jelena Nikolic Khatatbeh

Dissertations

This dissertation explores how the Russo-Ukrainian war has transformed the lives, identities, and resistance strategies of Ukrainian women. Drawing on in-depth, semi-structured interviews with forty-five Ukrainian women conducted between March 2022 and February 2023, it analyzes how everyday survival, political thought, and civic activism have changed during the war. Using a modified grounded theory approach and a feminist lens, this study examines women’s psychosocial experiences, their evolving political consciousness, and their active roles in humanitarian, cultural, and cyber resistance.Findings reveal that the war simultaneously reinforced and disrupted traditional gender expectations. While Ukrainian women dealt with increased domestic and caregiving burdens …

Accelerating Relationship Discovery In Chronic Lower Back Pain Through Knowledge Graph And Ontology Enhanced Large Language Models, Damon Lin

Master's Theses

Chronic lower back pain (cLBP) is a widespread public health burden linked to anxiety, depression, and opioid addiction. Interventions aimed at treating cLBP have shown minimal improvements in pain outcomes, leading researchers to reexamine our understanding of cLBP through constructing a causal model. However, constructing causal models through Randomized Controlled Trials are often unfeasible, and relying on domain expertise requires extensive and time-consuming research, posing a serious bottleneck for designing effective treatments. To accelerate this process, we apply Knowledge Graphs, Ontologies, and Large Language Models (LLMs) to aid researchers in determining possible causal relationships. First, we demonstrate how LLMs can …

Generalized Detection Of Animal Behavior Using Accelerometers, Alexander J. Arrieta

Master's Theses

Animal mounted sensors are becoming increasingly used to passively monitor both domestic and wild animals. Advances in lightweight accelerometer and GPS technology have allowed many animals to be fitted with high accuracy sensors for extended periods of time. This leads to new opportunities to study animal behavior without direct observation. However, interpreting the raw data is difficult due to the high volume and missing context of the information. Machine learning techniques excel at extracting information from raw data streams and are excellent candidates for processing the sensor data. However, due to large variance in how different animals execute the same …

Per- And Poly-Fluoroalkyl Substances Fate Across California Wastewater Treatment Plants: A Meta-Analysis Of Formation, Distribution, And Persistence, Elizabeth Reineke

Master's Theses

Per- and poly-fluoroalkyl substances (PFAS) are persistent contaminants that move through municipal and industrial wastewater systems with limited attenuation. As a result, they are increasingly detected in drinking water supplies. Despite growing regulatory concern, a systems-level understanding of PFAS behavior across multiple treatment facilities and watershed settings remains incomplete. This study compiled influent, effluent, and biosolids datasets from more than one hundred California wastewater treatment plants to evaluate PFAS occurrence, transformation, and partitioning across diverse treatment configurations. Analytical steps included calculating removal efficiencies, assessing precursor-to-product oxidation, evaluating co-contaminant correlations, and grouping facilities by process design to quantify configuration-specific fate patterns. …

Hallucination Techniques For Self-Supervised Synthetic Datasets For Mobile Robots, Wyatt D. Colburn

Master's Theses

Classical techniques in autonomous navigation struggle in tightly constrained spaces. Machine learning has been shown to perform better in these difficult environments but most techniques require large amounts of navigation experience for training. Using a new machine learning paradigm learning from hallucination (LfH), training data can be collected in a safe environment and not require supervision. Data is collected in real time while an agent performs a random walk in free space, supervision is not required as there are no obstacles for the robot to run into. After a random walk a post processing pipeline will hallucinate a safety corridor …

Birds In Changing Landscapes: Community Diversity And Breeding Phenology, Lindsay Peria

Master's Theses

Humans are altering the environment in varied and widespread ways. Increasing urban and agricultural development causes changes to habitat and resource availability as well as changes to the sensory environment via light and noise pollution. Climate change also causes more extreme temperature anomalies, storms, and droughts that challenge birds in a multitude of ways. In this thesis, we assessed these impacts of these changes on a local and continental scale. In the first chapter, we assessed the impact of natural, anthropic, and structural characteristics of viticultural land on both taxonomic and structural avian diversity. Loss of habitat from agriculture is …

Associations Between Feed Efficiency And Feeding Behavior In Yearling Angus Bulls In A Competitive Environment, Amelia Katz

Master's Theses

Feed efficiency in beef cattle is a complex trait shaped by physiological, behavioral, and social factors that influence how animals access and utilize feed resources. This study examined feed efficiency classification and how it is influenced by feeding behavior, competitive interactions, and bunk approach patterns in yearling Angus bulls housed in a drylot over two years. Bulls were grouped into high and low feed efficiency categories using residual feed intake and feed conversion ratio within pen and year (n = 40 per year; n = 40 per treatment). Continuous electronic monitoring quantified feeder visitation, feeding duration, intake, and rate. A …

Serum Concentrations Of Inflammatory Markers And Selected Nutrient Metabolites In Holstein And Jersey Cows Across The Transition Period, Avery Orr

Master's Theses

The transition period, spanning the weeks surrounding parturition, requires dairy cows to undergo synchronized metabolic, endocrine, and immunological adjustments that support the onset of lactation but also heighten disease susceptibility. Although individual inflammatory or metabolic biomarkers have been extensively studied, the integrated immunometabolic dynamics that occur across this period and the difference between breeds is not fully understood. The objective of this study was to characterize longitudinal patterns in key nutrient metabolites and inflammatory markers in Holstein and Jersey cows and assess how these systems interact across the transition period.

A total of 63 Holstein and Jersey cows were sampled …

Testing For Local Adaptation In Sympatric Monkeyflowers Growing In Serpentine Seeps And Adjacent Rock Outcrops, Avin Niknafs

Master's Theses

Divergent selection across heterogeneous environments can drive ecological differentiation and the evolution of locally adapted populations, even when gene flow occurs. We tested for local adaptation between two sympatric monkeyflowers, Erythranthe guttata and E. serpentinicola (formerly Mimulus), which co-occur on the central coast of California. Erythranthe guttata is a perennial species primarily found in moist serpentine seeps, whereas E. serpentinicola is an annual species restricted to adjacent serpentine rock outcrops. Using a reciprocal transplant experiment with second-generation, greenhouse-derived seed, we quantified germination, survival, and reproductive success across contrasting microhabitats. We further evaluated the relative contributions of species, lineage, …

The Effects Of Yoga In The Classroom On Social-Emotional Learning And Student Focus, Emily Wall

Capstones, Research Projects, and Curricula

This study investigated how students respond to brief yoga and breathwork practices being introduced in a high school biology classroom, specifically looking at the impacts to students’ social-emotional competencies, engagement, and academic focus. The theory of action guiding this inquiry was that embedding yoga practices into the classroom would decrease physical tension, decrease anxiety, increase self-esteem, and improve concentration, thus supporting their ability to engage and focus during learning tasks. The inquiry examined how students respond to a daily 10-minute yoga sequence implemented over three weeks (six class sessions). Data was collected through multiple tools, including a researcher-designed pre- and …

Terrestrial And Lacustrine Organic Matter Biomolecular Transformations Via Thermal Maturation And Oxidative Degradation, Louis Connor Bondurant

Chemistry & Biochemistry Theses & Dissertations

The most abundant source of fossil fuel-forming kerogen on Earth is classified as Type II kerogen, believed to originate from marine biomass. However, a significant amount of lacustrine and terrigenous carbon is transported to the ocean through fluvial discharge. Less than half of the exported organic carbon is observed in coastal regions and the open ocean. There is a great need for an explanation of what is happening to this organic carbon during transport and deposition. Traditionally, the marine organic matter in coastal regions accumulates to eventually be buried and transformed into petroleum over millions of years due to its …

Exploring Bonding Through Outdoor Recreation In Foster Care Utilizing Attachment Theory, Casandra L. Barnes

Communication & Theatre Arts Theses

As of 2024, there are over 328,000 children in foster care in the United States (AFCARS, 2024), many of whom experience disrupted attachments and trauma that hinder their ability to form healthy relationships. Drawing from attachment theory, this study explores how outdoor recreational activities can facilitate bonding between foster parents and their foster children. Using a mixed method approach this explorative research project consisted of two phases: a pilot study at the Virginia zoo involving foster families participating in a cooperative outdoor activity and completing post event surveys, and semi-structured interviews with foster parents to examine lived experiences of bonding …

Human Identification And Action Recognition Using Small Data And Deep Domain Adaptation, Alexander M. Glandon

Electrical & Computer Engineering Theses & Dissertations

Human identification and human action recognition problems are two important research areas for real-world security and surveillance applications. In both human identification and action recognition, it is necessary to operate by collecting small datasets in the field, possibly in a short time window of observation. This dissertation studies and develops computational modeling and high-performance machine learning (ML) and deep learning (DL) models for human identification and human action recognition using small amounts of data. These methods and computational models may be useful for different security and surveillance applications.

This dissertation on human recognition develops a ML computational model to estimate …

Comparative Analysis Of Emergent Behaviors Of Three Drone Swarm System Models For Targeting Using Agent-Based Modeling And Simulation, Arsenio T. Gumahad Ii

Engineering Management & Systems Engineering Theses & Dissertations

This dissertation introduces a novel computational simulation framework for evaluating the emergent behaviors of three swarm drone models using Agent-Based Modeling and Simulation (ABMS). The three swarm models are a Leader-Follower swarm model based on Bruckstein's antline theory, a Flocking model based on a simplified Reynolds 'Boids’ model, and a Stigmergic model with pheromone-based coordination. The primary objective of the simulation is to evaluate the performance of these models in delivering a user-defined number of drones of each type to a target area of interest in four separate scenarios, resulting in 50,000 separate simulation trials. Each scenario was structured to …

The Impact Of Eyewear On Early And Mid-Latency Erp Components Of Face Processing, Alewa T. Anaya

Electronic Theses, Projects, and Dissertations

Facial recognition plays an important role in human survival and social communication. It relies on fast and efficient visual processing in the brain, as reflected by the P100 and N170 event-related potential (ERP) components. These ERPs are early and mid-latency stamps of facial recognition that reflect visual detection and structural processing of faces. Previous studies have shown that the N170 is sensitive to disruptions in facial configuration, such as inversion or partial face obscuration caused by masks. The present study, however, examines a common yet overlooked visual addition that may influence the N170: eyewear. Specifically, this study investigates how regular …

Robustness Of Network Inference Algorithms Under Network Class Misspecification, Roberto Ceja

Electronic Theses, Projects, and Dissertations

Inferring phylogenies in the presence of hybridization remains a difficult problem. As a result, many current methods for reconstructing phylogenetic networks are restricted to a simple class of networks known as level-1. This restriction arises from theoretical considerations rather than empirical evidence, with real data possibly arising from complex networks. In this work, we evaluate the robustness of two level-1 network inference methods, SNaQ and NANUQ+, through a simulation study, when the input data originates from a more complex network. Specifically, we investigate whether these methods can accurately recover important features of the true species network, such as the circular …

Requirement Of Prepiilin Peptidase Activity For Variovorax Paradoxus Eps Flp/Fap Pilus Assembly, Jenny L. Lanthripp

Electronic Theses, Projects, and Dissertations

Variovorax paradoxus is a Gram-negative aerobic bacterium present in both soil and the human oral microbiome. This species contributes to biotransformation of xenobiotics and promotes plant growth. Interestingly, although the genome of strain EPS lacks the flagellum operon annotated in strain S110, the microbe remains motile, and flagella can be observed microscopically. To investigate this unexpected phenotype, motility mutants were enriched from a Tn5 mutant library using a liquid culture settling strategy. A total of 122 isolates were screened for motility defects, and 57 exhibited significantly reduced motility compared to the wild type. Rescue cloning revealed that over 60% of …

Potential Adverse Effects On Lifestyle And Food Intake Pattern In Night Shift Workers, Stacie L. Coleman

Electronic Theses, Projects, and Dissertations

Abstract

Night shift work is prevalent in healthcare and other industries, yet its effects on lifestyle factors such as food intake, sleep quality, and mental health remain underexplored. This study investigates the impact of night shift schedules on nurses’ wellness, focusing on eating patterns, sleep, physical activity, and mood. A sample of 13 nurses were divided into two shift groups: day (n=5) and night (n=8); and they completed a questionnaire to assess the variables related to their behavior, food choices, eating habits, sleep patterns.

The results of previous research studies indicate that night shift nurses are more likely to adopt …

Characterization Of A New Domestic Feline Macrophage Cell Line To Study Molecular Mechanisms Of Feline Coronavirus, Adam Espinoza

Electronic Theses, Projects, and Dissertations

Feline Coronavirus (FCoV) is a veterinary pathogen common in domestic cats. FCoV is transmitted via the fecal-oral route, results in mild enteric symptoms, and is considered feline enteric coronavirus (FECV). During infection the virus may transition from infecting host enteric epithelial cells to infecting host macrophages, resulting in a lethal systemic disease, and the virus is now considered feline infectious peritonitis virus (FIPV). FIPV arises in an infected individual, is not shed in the feces, and is not transmitted from cat to cat. There is limited research defining the host molecular mechanisms driving FCoV lethal outcomes, with few feline cell …

Phosphorylation At S1288 Of Leukemia Associated Rhogef (Larg/Arhgef12) Induces Plasma Membrane Localization And Promotes Binding And Activation Of Rhoa, Won Seok Yang, Neda Z. Ghanem, Steven D. Scahill, Maisel J. Caliva, Hannah Röttig, Annika Fitz, Michelle L. Matter, Joe W. Ramos

School of Graduate Studies Faculty Publications

Leukemia-associated RhoGEF (LARG) is a guanine nucleotide exchange factor (GEF) known for its specificity toward Ras homolog family member A (RhoA). LARG plays a crucial regulatory role in various cellular processes such as migration, proliferation, invasion, and metastasis by facilitating the exchange of GDP to GTP on RhoA. Phosphorylation of LARG at S1288 by ribosomal S6 kinase 2 (RSK2) promotes RhoA activation. However, the precise mechanism remains unclear. Here, we demonstrate the essential role and mechanism by which S1288 phosphorylation facilitates LARG-mediated invasiveness in response to epidermal growth factor (EGF). Upon EGF stimulation, RSK2 phosphorylates LARG at S1288, thereby promoting …

Training Aba Service Providers To Use Phonemic Transcription: A Comparison Of Two Alphabets, Kristin Marie Albert

Theses and Dissertations

Applied behavior analysis (ABA) service providers regularly develop speech and shape articulation when working with minimally vocal children with autism. However, these providers usually have not received training in related subject areas as part of their certification coursework. This results in a gap between their scopes of competence and practice. The present study aimed to lessen this gap by training ABA service providers to take phonemic transcription. The author, in consultation with a speech-language pathologist, developed and implemented a computer-based programmed instruction training package. Studies 1 and 2 employed a concurrent multiple-probe design with an embedded adapted alternating treatments design …

Understanding Hepatitis C Risk And Harm Reduction Needs Among People Who Smoke Drugs: A Client-Centered Qualitative Inquiry, Maxwell Gustin

Anthropology

The spread of Hepatitis C virus (HCV) via non-injectable equipment is plausible, making people who smoke drugs (PWSD) at-risk for possible infection and transmission. While much research has been conducted into injection drug use (IDU), likely due to the rising opioid epidemic, limited studies focus on the risks PWSD face. Currently, state-funded syringe service programs (SSPs) provide harm reduction education and materials to combat these risks. Safer smoking initiatives have been created and are widely used by PWSD, and have access to SSPs. To gain better insight into the HCV and non-HCV-related risks and burdens PWSD face, a qualitative study …

Exploring Life History Trade-Offs In The Facultative Eusocial Bee Megalopta Genalis, Ana Gutiérrez Zuluaga

All Graduate Theses and Dissertations, Fall 2023 to Present

Organisms face many trade-offs in how to invest in these traits. One example is the trade-off between reproduction and longevity. Queens of social insects seem to escape this trade-off, producing thousands of eggs daily, despite having a longer lifespan than their workers. Some hypotheses have been proposed to explain this phenomenon, such as an evolutionary change in the molecular pathways that regulate queen investment in maintenance, growth, and reproduction. An alternative hypothesis suggests that the tradeoffs are rarely observed in queens due to the extensive resources provided by the help of workers. While these hypotheses have supporting evidence, they have …

Enabling Scalable Camera-Based Streamflow Monitoring With Serverless Cloud Computing, Sajan Neupane

All Graduate Theses and Dissertations, Fall 2023 to Present

This thesis introduces a modern and automated method for monitoring rivers and streams using cameras and cloud-based software tools. Traditional monitoring methods often rely on sensors placed directly in the water, which can be expensive, hard to install, and difficult to maintain, especially in remote or rough terrain. In contrast, this approach uses cameras to observe water flow from a distance, offering a safer and more cost-effective alternative. However, camera systems come with their own challenges, such as collecting large amounts of image data, transferring it reliably from the field, and analyzing it quickly enough to be useful. This research …

Analysis On Modifications To An Outdoor Field-Scale Rotating Algal Biofilm Reactor With A Focus On Biomass Productivity And Power Usage, Davis R. Haag

All Graduate Theses and Dissertations, Fall 2023 to Present

A rotating algal biofilm reactor (RABR) was operated outdoors for one calendar year to see how the microalgae grown were affected. The algae are grown in wastewater that contains nitrogen and phosphorous, which are elements found in fertilizer. The design used was modified from a previous design, and this project tested the modifications to see if they improved how much microalgae were grown. In February 2025, the objectives of the field study were updated to focus on growing as much microalgae as possible instead of removing as much nitrogen and phosphorous as possible. This updated design increased the fluid flow …