Digital Commons Network^™

Electrostatic Effects On Tau Nanocondensates, Phoebe S Tsoi, Lathan Lucas, Derek Rhoades, Josephine C Ferreon, Allan Chris M Ferreon

Faculty, Staff and Students Publications

Biomolecular condensates (BMCs) are membrane-less protein compartments with physiological and pathological relevance. The formation of BMCs is driven by a process known as liquid-liquid phase separation (LLPS), a field that has largely focused on the study of micron-sized condensates. However, there have been recent studies showing that proteins that undergo LLPS also form nanometer-sized condensates. These nanometer-sized condensates, or nanocondensates, are distinct from microcondensates and potentially exhibit more relevance in cell biology. The field of nanocondensate research is in its infancy, with limited biophysical studies of these structures. Here, we studied condensate formation and dissolution of wild-type and disease-linked (hyperphosphorylated …

Overexpression And Biophysical And Functional Characterization Of A Recombinant Fgf21, Phuc Phan, Jason Hoang, Thallapuranam Krishnaswamy Suresh Kumar

Chemistry & Biochemistry Faculty Publications and Presentations

Fibroblast growth factor 21 (FGF21) is an endocrine FGF that plays a vital role in regulating essential metabolic pathways. FGF21 increases glucose uptake by cells, promotes fatty acid oxidation, reduces blood glucose levels, and alleviates metabolic diseases. However, detailed studies on its stability and biophysical characteristics have not been reported. Herein, we present the overexpression, biophysical characterization, and metabolic activity of a soluble recombinant FGF21 (rFGF21). The far-UV circular dichroism spectra of rFGF21 show a negative trough at 215 nm, indicating that the protein's backbone predominantly adopts a 13 sheet conformation. rFGF21 shows intrinsic tyrosine fluorescence at 305 nm. Thermal …

A Comparison Of Unconventional Microwave And Ultrasound-Assisted Extraction Methods Used For Flavonoids, Istiqomah Rahmawati, Daffa Hafiziaulhaq Azizi, Jihan Nafila Wibowo, Muhammad Reza, Boy Arief Fachri, Bekti Palupi, Meta Fitri Rizkiana, Helda Wika Amini, Ifan Ramadana, Felix Arie Setiawan

Makara Journal of Science

Cocoa pods (Theobroma cacao L.) are a rich source of flavonoids, which are natural antioxidants known for their health benefits. This study investigated the use of microwave-assisted extraction (MAE) and ultrasound-assisted ex-traction (UAE) to extract the maximum flavonoids and antioxidants from cocoa pods. MAE and UAE are efficient and sustainable methods for extracting bioactive compounds like flavonoids and antioxidants from cocoa pods, offer-ing faster extraction, reduced solvent use, and better compound preservation compared to conventional methods. These technologies unlock the untapped potential of cocoa pods for applications in food, cosmetics, and pharmaceuti-cals. The effects of extraction time (2–10 min), microwave …

Inter-Simple Sequence Repeats Application On The Identification Of Genetic Variation Of A New Indonesian Melon Cultivar “Melona”, Tantri Ajeng Salma Salsabila, Adib Fakhruddin Yusuf, Wiko Arif Wibowo, Prima Sekti Kusnanda, Budi Setiadi Daryono

Makara Journal of Science

The field of identification of molecular markers has seen significant advancements, with the Inter-Simple Sequence Repeat (ISSR) emerging as a prominent tool for characterizing genetic variation within species. This study focuses on the new Indonesian melon cultivar “Melona,” developed through selective breeding of commercial melons. This study aimed to assess the utility of ISSR markers in evaluating the genetic variation and stability of the “Melona” cultivar. Comparisons were made with established cultivars—‘Inthanon,” “Rich Sweetness,” “Chamoe,” and “Hikapel”—using five ISSR primers: UBC 807, UBC 808, UBC 810, UBC 812, and UBC 826. A total of 70 DNA fragments with a high …

The Expression Profile Of The Rank/Rankl/Opg Pathway In Breast Cancer Stem Cells Isolated From Breast Cancer Cell Lines, Mohamed M. Kamal, Hassnaa H. Elgohary, Sherine Maher Rizk, Nadine H. Maurice

Pharmacy

The RANK/RANKL/OPG signaling pathway plays a crucial role in breast cancer progression and metastasis. However, its expression patterns and potential implications in breast cancer stem cells remain poorly understood. This study aimed to characterize the expression profile of this pathway in breast cancer stem cells isolated from two distinct breast cancer cell lines: MDA‐MB‐231 and MCF‐7. Mammospheres (MS), representing breast cancer stem cells, were generated using agar‐coated 6 well tissue culture plates in suitable mammospheres culture conditions. Flow cytometric analysis showed enrichment of the CD44+/ CD24− subpopulations in the mammospheres cultures, with MDA‐MB‐231 exhibiting a higher percentage compared to MCF‐7. …

Effects Of Space Flight On Inflammasome Activation In The Brain Of Mice, Upal Roy, Roey Hadad, Angel A. Rodriguez, Alen Saju, Deepa Roy, Mario Gil, Robert W. Keane, Ryan T. Scott, Xiao W. Mao, Juan Pablo De Rivero Vaccari

Health & Biomedical Sciences Faculty Publications

Space flight exposes astronauts to stressors that alter the immune response, rendering them vulnerable to infections and diseases. In this study, we aimed to determine the levels of inflammasome activation in the brains of mice that were housed in the International Space Station (ISS) for 37 days. C57BL/6 mice were launched to the ISS as part of NASA’s Rodent Research 1 Mission on SpaceX-4 CRS-4 Dragon cargo spacecraft from 21 September 2014 to 25 October 2014. Dissected mouse brains from that mission were analyzed by immunoblotting of inflammasome signaling proteins and Electrochemiluminescence Immunoassay (ECLIA) for inflammatory cytokine levels. Our data …

Textbook Affordability Committee Minutes, March 12, 2025, Illinois State University, Textbook Affordability Committee

Textbook Affordability Committee

No abstract provided.

Analytical Electrochemistry Of Nickel, Platinum, And Platinum-Nickel Electrodepositions, Kyle James Troche

Nanoscience and Microsystems ETDs

Nickel and platinum electrodeposits have numerous applications in corrosion and wear-resistant coatings, electronic connectors, heat resistance, metal finishings, and catalysts. The addition of additives can vastly change the morphology of the deposition. Electrokinetic parameters can be calculated by conducting rotating disk electrode (RDE) experiments along with Koutecky-Levich analysis. Significant challenges need to be addressed to deposit uniform films of Pt-Ni alloys from a single co-deposition bath. The main challenge is platinum’s catalytic property to reduce the overpotential for the hydrogen generation reaction. An Investigation into methanol and carbon monoxide’s hydrogen generation reaction suppression was conducted. Electrodeposition of nickel, platinum, and …

Do Cheaters Prosper? Effect Of Externally Supplied Momentum During Resistance Training On Measures Of Upper Body Muscle Hypertrophy, Francesca Augustin, Jeff Nippard, Alec Pinero, Alysson Enes, Adam Mohan, Max Sapuppo, Max Coleman, Milo Wolf, Patroklos Androulakis Korakakis, Paul Swinton, Brad Schoenfeld

International Journal of Exercise Science

International Journal of Exercise Science 18(3): 329-342, 2025. Exercise technique, defined as the controlled execution of bodily movements to ensure an exercise effectively targets specific muscle groups while minimizing the risk of injury, is a resistance training (RT) variable frequently highlighted as critical to successful RT program outcomes, with proper technique suggested to play a role in maximizing muscle development. This study examined the effects of externally applied momentum on RT-induced muscular adaptations in the upper extremities. Thirty young adults were recruited to participate in a within-participant design, with one limb randomly allocated to perform biceps curls and triceps pushdowns …

American Option Pricing Using Generalised Stochastic Hybrid Systems, Evelyn Buckwar, Sascha Desmettre, Agnes Mallinger, Amira Meddah

Journal of Stochastic Analysis

No abstract provided.

Energy-Aware Clustering Using Intelligent Scheme For Heterogeneous Wireless Sensor Networks, Enaam A. Al-Hussain, Ghaida A. Al-Suhail

Karbala International Journal of Modern Science

Heterogeneous Wireless Sensor Networks (WSNs) involve nodes with varying capabilities, such as different energy levels, sensing ranges, and computational abilities, which enable them to execute different tasks professionally. Clustering techniques play a crucial role in improving energy efficiency and reliability in WSNs. The evolution of cluster based WSNs from homogeneous into heterogeneous techniques allowed the deployment of smart devices capable of performing complex operations in in diverse environments. However, the heterogeneity of nodes necessitates more sophisticated and adaptive algorithms to fully exploit these capabilities. This paper proposes a new protocol, referred to as IT2F-HLEACH, which integrates Interval Type-2 Fuzzy Logic …

Comprehensive Review On The Application Of Bio-Immunoinformatics In The Development Of Highly Ef-Fective New Candidate Vaccines Against Tuberculosis, Ahyar Ahmad, Andriansjah Rukmana, Miski A. Khairinisa, Dian A. E. Pitaloka, Rosana Agus, Rusdina B. Ladju, Tarwadi Ahmad, Astutiati Nurhasanah, Carina C. D. Joe, Muhammad N. Massi, Harningsih Karim, Irda Handayani, Siti Roszilawati Binti Ramli

Karbala International Journal of Modern Science

Tuberculosis (TB) remains a significant public health challenge worldwide. Currently, Bacillus Calmette-Guerin (BCG) is the only vaccine available for TB prophylaxis. However, the efficacy of the BCG vaccine against adult pulmonary TB is considered inconsistent. This condition encourages researchers to look for more effective options, such as subunit vaccines. This condition requires the development of a more effective subunit vaccine to protect active TB in productive and adult ages. There is an urgent need for more effective vaccines, as the Bacillus Calmette-Guérin (BCG) vaccine currently available has inconsistent efficacy and is only partially effective in adults. Bio-immunoinformatics, an interdisciplinary field …

Ras El-‘Amud, Jerusalem: Faunal Remains From The Intermediate Bronze And Middle Bronze Age Settlement, Nimrod Marom

Qadum: Journal of Excavation Reports from Israel

The small faunal assemblage from Ras el-‘Amud provides insights into the economic strategies of a rural settlement during a period of urban growth. A shift from a self-sufficient goat and pig economy to a specialized sheep-focused one is observed, likely driven by urban demand. Analysis of caprine skeletal remains suggests consumption patterns and potential ritualistic practices. The Middle Bronze IIA village exhibits a decrease in butchery waste, possibly due to changes in spatial organization or waste disposal practices. The assemblage offers valuable insights into the economic and social dynamics of rural settlements during the Middle Bronze Age.

March 11, 2025, Council On Graduate Studies

Minutes

No abstract provided.

Medical School Watercooler Newsletter - March 11, 2025, Lindsay Hughes

Watercooler Newsletter

This is the Tuesday, March 11, 2025 edition of the Frederick P. Whiddon College of Medicine's newsletter -- Watercooler.

Contents include:

• Borchert lab research challenges longstanding view of gene regulation
• Medical students and pediatrics residents present research at regional meeting
• Class 2025 to celebrate Match Day March 21
• First-year medical student to receive a community health leadership award

Competitive Language Use: A Linguistic Analysis Of Speech And Debate, Gina Iberri-Shea

Studies in Debate and Oratory

This study explores linguistic variation in competitive debate and prepared speech. A corpus of competitive public speech was collected and the situational characteristics of two contexts were analyzed. The linguistic characteristics of texts in each category were calculated along five dimensions of variation found across texts in English, and placed among other types of texts. Results show that three of the dimensions were able to distinguish between the two registers. The results indicate a clear direction for future work and implications for English for Academic Purposes instruction.

Engaging Biblical Criticism In The American South: A Local Church Approach, Adam Harris

Doctor of Leadership

Historical critical approaches to the Bible that reexamine doctrines of inerrancy are unheard of, dismissed, or viewed as a threat throughout Christian faith communities in Sumner County, TN. This underutilizes a credible method of interpretation for Christians who have only been exposed to views that tightly hold biblical inerrancy. The lack of awareness and disregard for aspects of biblical criticism in local churches by leaders and congregation members in Sumner County, TN, is multifaceted.

Most stakeholders in this NPO context have backgrounds in conservative American Evangelical Protestant congregations. They are either struggling with or reexamining their inherited beliefs due to …

Transcriptome Complexity Disentangled: A Regulatory Molecules Approach, Amir Asiaee, Zachary B Abrams, Heather H Pua, Kevin R Coombes

2020-Current year OA Pubs

Transcription factors (TFs) and microRNAs (miRNAs) are fundamental regulators of gene expression, cell state, and biological processes. This study investigated whether a small subset of TFs and miRNAs could accurately predict genome-wide gene expression. We analyzed 8895 samples across 31 cancer types from The Cancer Genome Atlas and identified 28 miRNA and 28 TF clusters using unsupervised learning. Medoids of these clusters could differentiate tissues of origin with 92.8% accuracy, demonstrating their biological relevance. We developed Tissue-Agnostic and Tissue-Aware models to predict 20,000 gene expressions using the 56 selected medoid miRNAs and TFs. The Tissue-Aware model attained an R2 of …

Structural Basis For Dimerization And Activation Of Uvrd-Family Helicases, Ankita Chadda, Binh Nguyen, Timothy M Lohman, Eric A Galburt

2020-Current year OA Pubs

UvrD-family helicases are superfamily 1A motor proteins that function during DNA replication, recombination, repair, and transcription. UvrD family monomers translocate along single-stranded (ss) DNA but need to be activated by dimerization to unwind DNA in the absence of force or accessory factors. However, prior structural studies have only revealed monomeric complexes. Here, we report the first structures of a dimeric UvrD-family helicase,

Global Aging & Geriatric Experiments In Bipolar Disorder (Gage-Bd): Building A Global Consortium Benefiting People With Bipolar Disorder In Later Life, Martha Sajatovic

Faculty Scholarship

Purpose of Review: Findings from the Global Aging & Geriatric Experiments in Bipolar Disorder (GAGE-BD) project, including sociodemographic and clinical information from older age bipolar disorder (OABD) and healthy participants around the globe (approximately N = 5000) were reviewed. Data was collected in multiple waves to create a large integrated dataset. Recent Findings: BD does not seem to fade with age. BD subtype and early/late onset did not show significant differences in daily functioning. Physical comorbidities were more frequent in OABD compared with controls. Women with OABD had an earlier age at onset and more psychiatric hospitalizations. Summary: GAGE-BD is …

Academic Senate - Agenda, 3/11/2025, Academic Senate

Academic Senate Agendas

No abstract provided.

The Minister’S Midlife Quest: A Relational Coaching Framework, Esther E. Edwards

Doctor of Leadership

Midlife arrives with a tsunami of life transitions and a myriad of internal shifts that need processing. Furthermore, the lengthening lifespan, negative impacts of ageism and inadequate framing of midlife’s intention can leave the midlife journeyer unaware of the deep transformation, fresh purpose, and future prospects that God intends the journey to bring.

Ministers are no exception to this journey. However, they contend with the unique pressures of being a constant Godly example for others often with few trusted relationships to authentically process with. In addition, many lack the necessary awareness of tools that foster strategic approaches to weathering transitions …

Stem Ability Perceptions, Basic Needs Satisfaction, And Intrinsic Motivation In Adolescents: The Role Of Inclusive Perceptions In Self-Determination, Angelina Joy, Adam Hartstone-Rose, Jerica Knox, Channing J. Mathews, Jacqueline Cerda-Smith, Kelly Lynn Mulvey

Purdue Center for Early Learning Faculty and Staff Publications

Current work suggests that basic psychological needs are related to higher intrinsic motivation, which in turn, can promote more positive academic outcomes. However, few studies have examined how perceptions around one’s abilities in science, engineering, technology, and math (STEM) are related to intrinsic motivation and what role needs satisfaction plays in this association. This study assessed adolescents’ (N = 285, 56.1% female, Mage = 15.76 years, SD = 1.24) STEM ability perceptions, basic needs satisfaction, and intrinsic motivation. A path analysis was used to examine the association between STEM ability perceptions, basic needs satisfaction, and intrinsic motivation in …

Everyday Life Information Behavior, Health Information Seeking, And Hiv And Sti Awareness Among People Who Inject Drugs (Pwid), Nafiz Z. Shuva, Radia Taisir, Parvez Sazzad Mallick

Publications and Research

This study aims to explore the everyday life and health information behavior of people who inject drugs (PWID) in Bangladesh, as well as the effectiveness of HIV and STI informational programs in promoting safe injecting and sexual behavior. The study received 97 completed survey responses and conducted two focus group discussions (FGDs) with 13 PWID. Using de Certeau's everyday life practices framework, the study aims to understand the tactical information strategies of PWID in the context of a developing country where access to government services and trust in authorities may be significantly limited among marginalized populations. PWID's information needs focused …

Transcriptional Profiles Reveal Physiological Mechanisms For Compensation During A Simulated Marine Heatwave In Yellowtail Kingfish (Seriola Lalandi), Sharon E. Hook, Ryan J. Farr, Jenny Su, Alistair J. Hobday, Catherine Wingate, Lindsey Woolley, Luke Pilmer

Fisheries Research Articles

Background

Changing ocean temperatures are already causing declines in populations of marine organisms. Predicting the capacity of organisms to adjust to the pressures posed by climate change is a topic of much current research effort, particularly for species we farm or harvest. To explore one measure of phenotypic plasticity, the physiological compensations in response to heat stress as might be experienced in a marine heatwave, we exposed Yellowtail Kingfish (Seriola lalandi) to sublethal heat stress, and used the transcriptome in gill and muscle, benchmarked against heat shock proteins and oxidative stress indicators, to characterise the acute heat stress …

Structural Basis For Intrinsic Strand Displacement Activity Of Mitochondrial Dna Polymerase, Ashok Nayak, Viktoriia Sokolova, Sirelin Sillamaa, Karl Herbine, Juhan Sedman, Dmitry Temiakov

Department of Biochemistry and Molecular Biology Faculty Papers

Members of the Pol A family of DNA polymerases, found across all domains of life, utilize various strategies for DNA strand separation during replication. In higher eukaryotes, mitochondrial DNA polymerase γ relies on the replicative helicase TWINKLE, whereas the yeast ortholog, Mip1, can unwind DNA independently. Using Mip1 as a model, we present a series of high-resolution cryo-EM structures that capture the process of DNA strand displacement. Our data reveal previously unidentified structural elements that facilitate the unwinding of the downstream DNA duplex. Yeast cells harboring Mip1 variants defective in strand displacement exhibit impaired oxidative phosphorylation and loss of mtDNA, …

Exploratory Co-Design On Electronic Health Record Nursing Summaries: Case Study, Suhyun Park, Jenna L Marquard, Robin R Austin, Christie L Martin, David S Pieczkiewicz, Connie W Delaney

The Brown Foundation: Institute of Molecular Medicine

Background: Although electronic health record nursing summaries aim to provide a concise overview of patient data, they often fall short of meeting nurses' information needs, leading to underutilization. This gap arises from a lack of involvement of nurses in the design of health information technologies.

Objective: The purpose of this exploratory co-design case study was to solicit insights from nurses regarding nursing summary design considerations, including key information types and the preferred design prototype.

Methods: We recruited clinical nurses (N=33) from 7 inpatient units at a university hospital in the Midwestern United States using a purposive sampling method. We used …

Metabolic Signatures In Adipose Tissue Linking Lipophilic Persistent Organic Pollutant Mixtures To Blood Pressure Five Years After Bariatric Surgery Among Adolescents, Shudi Pan, Zhenjiang Li, Douglas I Walker, Brittney O Baumert, Hongxu Wang, Jesse A Goodrich, Sarah Rock, Thomas H Inge, Todd M Jenkins, Stephanie Sisley, Scott M Bartell, Stavra Xanthakos, Xiangping Lin, Brooklynn Mcneil, Anna R Robuck, Catherine E Mullins, Michele A La Merill, Erika Garcia, Max T Aung, Sandrah P Eckel, Rob Mcconnell, David V Conti, Justin R Ryder, Lida Chatzi

Faculty, Staff and Students Publications

Persistent organic pollutants (POPs) are lipophilic environmental contaminants accumulated in the adipose tissue. Weight loss interventions, such as bariatric surgery, can mobilize POPs from adipose tissue into the bloodstream. We hypothesized that this mobilization could contribute to increases in blood pressure among 57 adolescents with severe obesity undergoing bariatric surgery. POPs and metabolic features were measured from visceral adipose tissue collected during surgery using gas and liquid chromatography, coupled with high-resolution mass spectrometry. Blood pressure was assessed at baseline, 6 months, and 5 years post-surgery. We used quantile g-computation to estimate associations of POP mixtures with blood pressure changes. With …

Molecular Identification Of The Italian Soldiers Found In The Second World War Mass Grave Of Ossero, Barbara Di Stefano, Barbara Bertoglio, Filomena Melchionda, Monica Concato, Solange Sorçaburu Ciglieri, Alessandro Bosetti, Pierangela Grignani, Eros Azzalini, Yasmine Addoum, Raffaella Vetrini, Fabiano Gentile, Francesco Introna, Serena Bonin, Chiara Turchi, Carlo Previderè, Paolo Fattorini

Faculty, Staff and Students Publications

Background/objectives: DNA analysis is the most reliable method for the identification of human skeletal remains, especially the ones found in mass disasters or recovered from mass graves. To this aim, DNA was extracted from bones and teeth allegedly belonging to 27 Italian soldiers executed during the Second World War and exhumed from a mass grave in Ossero (Croatia).

Methods: A selection of 131 different bone samples (petrous bones, femurs, metacarpal, and metatarsal bones) and 16 molar teeth were used for DNA extraction. Autosomal and Y-chromosome STR profiles were determined using a conventional CE approach, while a panel of 76 microhaplotypes …

Conformational Free Energy Landscape Of A Glutamate Transporter And Microscopic Details Of Its Transport Mechanism, Sundar Thangapandian, Ashkan Fakharzadeh, Mahmoud Moradi, Emad Tajkhorshid

Chemistry & Biochemistry Faculty Publications and Presentations

Removing glutamate from the synaptic cleft is vital for proper function of the brain. Excitatory amino acid transporters mediate this process by uptaking the neurotransmitter from the synaptic cleft back to the cell after its release. The archaeal homolog, GltPh, an aspartate transporter from Pyrococcus horikoshii, presents the best structurally characterized model for this family of transporters. In order to transport, GltPh undergoes elevator-like conformational changes between inward-facing (IF) and outward-facing (OF) states. Here, we characterize, at an atomic level, the OF=+/- IF transition of GltPh in different apo/bound states using a combination of ensemble-based enhanced sampling techniques, employing more …