Digital Commons Network^™

Advancing Point-Of-Care Healthcare Diagnostics To Tackle Future Outbreaks And Pandemics: Nanotechnology-Driven Developments And Impact On Rapid Pathogen Detection, Naresh Mandal, Bidhan Pramanick, Tarun Kanti Bhattacharyya, Baljit Singh

Research Outputs: 2025-Present

No abstract provided.

Biophysical Characterization And Structural Insights Into The Complex Between The Extracytoplasmic Function Sigma Factor, Pupi, With The Antisigma Domain Of The Sigma Regulator, Pupr, From Pseudomonas Capeferrum, Tajnin Sultana, Robin N. Kutsi, Alexander Tischer, Siri Froiland, Elizabeth L. Gertz, Jaime L. Jensen, Matthew Auton, Sangita C. Sinha, Christopher L. Colbert

APC Fund Awardees

Gram-negative bacteria utilize siderophores to scavenge for environmental iron. Pseudomonas capeferrum produces the siderophores pseudobactin BN7/8, which trigger cell surface signaling (CSS). Iron import CSS pathways consist of an outer membrane TonBdependent transporter, an inner membrane sigma regulator, and a cytoplasmic sigma factor that activates transcription of target genes. The P. capeferrum sigma regulator, PupR, has an N-terminal antisigma domain (ASD). Previously, the PupR ASD was purified as a dimer in the absence of its cognate sigma factor, PupI. Here we use pulldown assays to demonstrate that His6-PupIFL interacts with the PupR ASD as well as with homodimer-disrupting mutants. We …

Investigating Metabolic Heterogeneity In Engineered Isogenic Microorganisms, Xinyue Mu

McKelvey School of Engineering Graduate Student Theses & Dissertations

Engineered microorganisms play an increasingly important role in the sustainable production of fuels, bioplastics, and other valuable biomaterials. Successful industrial deployment depends on maximizing product titers and ensuring process robustness at large scale. However, one of the major obstacles is non-genetic cell-to-cell heterogeneity arising from stochastic intracellular processes. Such heterogeneity can result in subpopulations that divert substrates toward growth rather than product biosynthesis, ultimately reducing yield and contributing to batch-to-batch inconsistency. To better understand the mechanisms by which cell-to-cell variation emerges, propagates, and impacts bioproduction, this dissertation investigated how intracellular energetic dynamics and stochastic biosynthetic noise contributed to metabolic heterogeneity …

Enhancing Public Health Strategies: Addressing The Rsv Burden In Alabama Through Clinical Treatment, Community Prevention, And Surveillance Approaches, Martha K. Findley Roper, Jennifer S. Dawson

Journal of Public Health in the Deep South

Introduction: Respiratory Syncytial Virus (RSV) is a highly contagious pathogen driving morbidity, hospitalizations, and economic burden. This review uses the epidemiological triad and National Wastewater Surveillance System data to assess transmission, viral load spikes in Alabama, and strategies for treatment and prevention.

Methods: A review of literature on supportive care, vaccination, monoclonal antibodies, and emerging nanomedicine was reviewed to evaluate strategies for reducing viral burden and complications.

Results: Trends from December 2024–January 2025 were assessed with National Wastewater Surveillance System data. Primary prevention includes immunization, hygiene, air quality maintenance, and early diagnostics. Tertiary care addresses complications such as secondary …

Early Intervention In Helminth Infections: Investigating Albendazole’S Effects On C. Elegans Egg Hatch Rate And Health, Giselle Domingo Diaz

Student Scholar Symposium

Over 1.5 billion of the poorest people are infected with soil-transmitted helminths (STH) such as whipworm, hookworm, and Ascaris which have devastating effects on human growth, nutrition, and cognition, perpetuating the cycle of poverty. The World Health Organization only approves four anthelmintic drugs to treat STH for mass drug administration (MDA), with Albendazole being the drug of choice. Due to these drugs only having two mechanisms of action, resistance to these drugs has been reported over the years. We aim to better understand STH susceptibility to drugs at multiple points in their life stage. Previously, the effects of Albendazole and …

Emergency Care Team Comfort And Experience With Harm Reduction For Opioid Use Disorder, Penelope J. Haydar, Tania D. Strout, Faen Faen

Journal of Maine Medical Center

Introduction: Harm reduction strategies are effective in mitigating risks associated with substance use. However, their implementation in emergency department (ED) settings has been limited. This study explored ED care team members’ experiences and comfort with harm reduction provision.

Methods: We conducted a cross-sectional study to assess ED care team members’ experiences and comfort levels (0–10 scale) with providing harm reduction resources to patients. An electronic survey was distributed to 446 care team members at a Level 1 tertiary care ED. Domains included prior harm reduction practice, comfort levels, perceived barriers to harm reduction provision, and perceived importance of various factors …

Evaluation Of Connections Clinic: The Bridge Program Located Within The Preble Street Learning Collaborative, Emily Caron, Emily Follo, Robin E Barnard, Alec Luro, Adam Normandin

Journal of Maine Medical Center

Problem: The opioid epidemic continues, and overdose rates throughout the United States remain high. Only 22% of people diagnosed with opioid use disorder (OUD) access medications for OUD (MOUD). Bridge clinics may improve access to MOUD, specifically buprenorphine. Individuals experiencing homelessness (IEH) face additional barriers to accessing care, though little is known about bridge clinics specifically focused on IEH. The bridge clinic embedded within the MaineHealth–Preble Street Learning Collaborative, called Connections Clinic (CC), aims to address unmet health care needs of IEH in Portland, Maine, by providing low-barrier health care and access to buprenorphine.

Approach: We analyzed demographics, health access, …

Bartonella Endocarditis Unmasking Heart Failure, Nephrotic Syndrome, And Pancytopenia In Repaired Tetralogy Of Fallot: A Case Report, Cani Greg L. Garsuta

Journal of Maine Medical Center

Introduction: Bartonella species are notable causes of culture-negative endocarditis, particularly in patients with prosthetic valves or congenital heart disease. These infections can present insidiously, mimicking other systemic illnesses and posing considerable diagnostic challenges due to their fastidious nature.

Clinical Findings: A person in their 30’s with repaired tetralogy of Fallot and a Melody pulmonary valve presented with acute biventricular heart failure, nephrotic-range proteinuria, and pancytopenia. Initial assessments suggested cardiorenal syndrome. Despite negative routine cultures, persistent renal abnormalities and hypocomplementemia raised suspicion for glomerulonephritis. Subsequent polymerase chain reaction analysis of blood returned positive for Bartonella henselae, and positron emission tomography-computed …

Cotton Town Blues: An Investigation Of Social Inequality, Diet, And Health In A 19th Century Industrializing Population—St Peter’S Churchyard, Blackburn, Lancashire, Andy Barlow, Catriona Pickard, Linda Fibiger, Kerry L. Sayle, Sarah Schaeffer, Emma Smith, Julie Franklin, Brooke Carter, Lea Perrino, Clive Bonsall

Human Biology Open Access Pre-Prints

Historical accounts document that the shift from rural settlement to urban settings and from employment in cottage industries to factory working at the turn of the 19th century had a negative impact on the lower classes.

In 2015, the relocation of the cemetery of St Peter’s Churchyard in Blackburn, Lancashire, provided an opportunity to study the effects of industrialization on the diets and health of an early to mid-19th-century population through osteological and biomolecular analyses. Those interred at St Peter’s represent a socioeconomic cross-section of the church’s parishioners. In this paper, we present an evaluation of the lifetime dietary biographies …

Fpga-Accelerated Computational Photon Counting, Chibueze Onyeador

McKelvey School of Engineering Graduate Student Theses & Dissertations

Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful tool for biomedical re- search. It leverages fluorescence of biological samples to visualize and characterize biological dynamics and molecular interactions. FLIM utilizes fluorescence lifetime, the average time a molecule stays in its excited state after absorbing a photon, to investigate the dynamics of biological samples.

Ultra-fast and precise (ps-ns) timing fluorescence acquisition techniques suffer from different trade-offs. Some methods suffer from high dead times, decreased accuracy in fluorescence lifetime calculations, and high data volumes and transfers. Finding a new method that can provide the best performance while experiencing low dead times, adequate …

Ratio Quantification Of Geranium And Rose Essential Oil Mixtures Via Leep Learning Analysis Of Complex Raman Spectra, Jia Wei Tang, Yong Xuan Hong, Jie Chen, Yun Yun Xie, Zhang Wen Ma, Qing Hua Liu, Liang Wang

Research outputs 2022 to 2026

Due to their high economic value, essential oils (EOs) are increasingly subject to adulteration, posing significant challenges for quality assessment and control. Owing to its excellent molecular specificity, Raman spectroscopy has been widely employed for the analysis and evaluation of EO products. In this study, we collected a total of 2700 Raman spectra comprising rose essential oil (REO, n = 900), geranium essential oil (GEO, n = 900), and their mixtures (n = 900). We first constructed a conventional convolutional neural network to distinguish spectra corresponding to varying EO mixing ratios. Interpretability analysis revealed that spectral peaks at 800, 1000, …

Incidence Of Bacterial Chondronecrosis With Osteomyelitis Lameness In Commercial Duck Flocks In Mojokerto, Indonesia, Andi Asnayanti, Siti Azizah, Anif Mukaromah Wati, Ahmad Ridwan, Ahmad Arman Dahlan, Dinda Rosalita, Anh Dang Trieu Do, Adnan Alrubaye

Poultry Science Faculty Publications and Presentations

Bacterial Chondronecrosis with Osteomyelitis (BCO) lameness is an infection of opportunistic bacteria in the structural skeletal bones impacting multiple animal species, particularly poultry species. BCO lameness results in significant financial losses to industrial poultry production and increases the risk of foodborne illnesses, posing a major threat to consumers' food safety. As BCO lameness is an inherent risk of fast body weight gain in poultry species, especially broiler chickens, abundant studies have been conducted in broilers and turkeys. Nevertheless, BCO lameness incidence in ducks remains elusive. Thus, this is the first survey investigating the prevalence of BCO lameness cases in ducks. …

Her2-Low And Her2-Ultralow Metastatic Breast Cancer And Trastuzumab Deruxtecan: Common Clinical Questions And Answers, Nusayba A Bagegni, Karthik V Giridhar, Daphne Stewart

2020-Current year OA Pubs

Approximately 80% of invasive breast cancers are classified as human epidermal growth factor receptor 2 (HER2)-negative; however, many of these tumors have detectable levels of HER2 surface expression. Trastuzumab deruxtecan (T-DXd) is a HER2-directed antibody-drug conjugate with a membrane-permeable payload that is cytotoxic to both HER2-expressing tumor cells and neighboring cells via the bystander antitumor effect. T-DXd has shown significant antitumor activity in clinical trials for patients with HER2-positive (immunohistochemistry [IHC] 3+ or IHC 2+/in situ hybridization [ISH]+) breast cancer. In addition, the results of the DESTINY-Breast04 trial demonstrated the clinical benefit of T-DXd in patients with HER2-low (IHC 1+ …

Covid-19 Event Impact: Religious/Spiritual Coping And Posttraumatic Growth In Adult Female Survivors Of Childhood Sexual Abuse, Mindy Barrett Perlman

Doctoral Dissertations and Projects

The COVID-19 pandemic presented a unique collective trauma that has disproportionately impacted vulnerable populations, including adult female survivors of childhood sexual abuse (CSA). This quantitative, non-experimental, correlational study examined the relationship between spiritual/religious coping and posttraumatic growth (PTG) among this population, while also exploring the psychological impact of the COVID-19 pandemic and the mediating role of religious/spiritual coping. Grounded in Tedeschi and Calhoun’s Posttraumatic Growth Theory and Pargament’s Religious Coping Theory, this study utilized the Posttraumatic Growth Inventory (PTGI), the Brief Religious Coping Scale (Brief RCOPE), and the Impact of Event Scale–Revised (IES-R) adapted for COVID-19 to assess trauma exposure, …

Methods For The Discovery Of Antibodies Toward Junin Virus Utilizing Yeast Surface Display, Jonathon E. Corral

Doctoral Dissertations and Projects

The importance of this study is to perform antibody discovery as a means to derive novel preventative and therapeutic antibody countermeasures against the Junín virus, to alleviate the potential risk of a global pandemic. The purpose of this methodological study was to develop a protocol that utilizes yeast surface display to discover antibodies, from naïve human antibody libraries, that can bind the Junín Virus antigen. The methodology of yeast surface display employs the galactose-inducible GAL1 promoter to synthesize and express antibody proteins bound to the Aga2p and Aga1p and anchored to the cell wall by glycosyl phosphatidylinositol motif (GPI). The …

The Impact Of Street Medicine Programs On Addressing Poor Health Outcomes In Unhoused Populations, Lori R. Kelley

Doctoral Dissertations and Projects

The purpose of this phenomenological study was to understand the problem of poor health among unhoused populations based on the perspectives of outreach workers and shelter staff. This study was conducted in Monroe County, Indiana. The theory guiding this study was social ecological theory by Urie Bronfenbrenner. Social ecological theory was used to understand how social ecological layers impact poor health in unhoused populations. Hermeneutic phenomenology was used with purposive maximum variation sampling to gather seven study participants with lived experience of working with unhoused populations. Data collection included one-on-one interviews, observation, and documentation review. After data collection was completed, …

Comparison Of Cost, Accessibility, Speed, And Efficiency Of Modern Microbial Detection Methods For Wastewater Surveillance And Their Potential Benefits In Low-Resource Settings, Gloria Rodriguez, Davida Smyth

Masters Theses (Archived)

During the COVID-19 pandemic, wastewater surveillance helped predict the emergence of several waves of the SARS-CoV-2 virus, sometimes before clinical detection, alerting public health authorities to investigate. As human fecal material can contain the nucleic acids of both DNA- and RNA-based pathogens, new methods isolating total nucleic acid (TNA) can be used to detect a wider variety of pathogens and genetic targets. Wastewater Surveillance is faster, cost effective and scalable. This study compared three commercially available TNA extraction kits: Promega Rapid Capture Beads (Rapid), Maxwell RSC Enviro Total Nucleic Acid (Vacuum), and Nanotrap® Microbiome A and B Enviro Water Protocol …

What Impact Did Covid-19 Have On College Students With Social Anxiety Disorder: A Phenomenological Research Study, Kenneth R. Reines

Doctoral Dissertations and Projects

This phenomenological study aimed to explore the impact the COVID-19 pandemic (2020-2023) had on college students diagnosed with social anxiety disorder (SAD). Guided by Lazarus and Folkman’s (1984) Stress and Coping Theory, this study explored how participants perceived and coped with the difficulties they experienced during this difficult period. Data was gathered through Zoom interviews with six former students with a clinical diagnosis of social anxiety disorder.. Participants’ experiences collectively reflected persistent psychological decline, characterized by long-term worsening of symptoms and a lasting psychological impact marked by the exacerbation of social anxiety disorder (SAD). Isolation and disconnection stemming from limited …

Clinical Practice Variation Among Pediatric Rheumatologists Treating Kawasaki Disease: Results Of A North American Survey, Daniel Ibanez, Bianca Lang, Julia Shalen, Ali Yalcindag, Linda Wagner-Weiner, Kenneth N Schikler, Shoghik Akoghlanian, Hulya Bukulmez, Kristen Hayward, Laura Berbert, Sivia Lapidus, Andrea A Ramirez, Cagri Yildirim-Toruner

Faculty, Staff and Students Publications

Background: The best treatment for children with KD who fail to respond to the first dose of IVIG (refractory KD) is currently unknown. The purpose of this study was to determine treatment practices of pediatric rheumatologists in North America who manage IVIG-refractory KD.

Methods: A 34-item web-based survey was sent to 102 randomly selected members of the Childhood Arthritis and Rheumatology Research Alliance (CARRA). The anonymous survey addressed the use of primary intensification as well as the treatment of IVIG-refractory KD.

Results: The response rate was 82%; 56% (all pediatric rheumatologists) completed the survey. Primary intensification was …

Baseline Ecg And Cardiovascular Outcomes In People With Hiv: Insights From Reprieve, Aya Awwad, Shashwatee Bagchi, Et Al.

2020-Current year OA Pubs

BACKGROUND: With antiretroviral therapy, people with HIV (PWH) have an increased burden of cardiovascular disease. The REPRIEVE (Randomized Trial to Prevent Vascular Events in HIV) trial demonstrated that pitavastatin reduces major adverse cardiovascular events (MACEs) among PWH at low to moderate traditional atherosclerotic risk. Electrocardiographic abnormalities are common in PWH, but little is known about their association with MACEs. We sought to examine whether baseline electrocardiographic abnormalities are associated with increased MACE risk among a global primary cardiovascular disease prevention cohort of PWH in REPRIEVE.

METHODS: In this observational analysis, entry electrocardiographic abnormalities were adjudicated and classified as major or …

2022u_Nhp48_Approved_Cm_Emsp-2244-Medical-Emergencies-I-Rev3, Undergraduate Curriculum Committee

All Approved Curriculum

No abstract provided.

2022u_Nhp48_Approved_Cm_Emsp-2244-Medical-Emergencies-I-Rev2, Undergraduate Curriculum Committee

All Approved Curriculum

No abstract provided.

2022g_Nhp122_Approved_Nc_Nurs_615v-Fnp-Systems-Iii, Graduate Council

All Approved Curriculum

No abstract provided.

2022g_Nhp118_Approved_Nc_Nurs_612v-Fnp-Pediatrics, Graduate Council

All Approved Curriculum

No abstract provided.

Economic Evaluation Of Ai-Assisted Technologies In Healthcare: A Systematic Review, Wen-Tung Wu, Yi-Wen Chao, Tsung-Kun Lin, Ching-Kuo Huang, Ping-Hsuan Hsieh

Journal of Food and Drug Analysis

Artificial intelligence (AI) technologies are increasingly integrated into healthcare, yet their economic value remains uncertain. Traditional economic evaluation methods may not adequately capture the unique features of AI, including dynamic model evolution, scalability, and broader societal impacts. This systematic review synthesized existing evidence on the cost-effectiveness of AI-based healthcare interventions and assessed the methodological rigor of published studies. A comprehensive search identified health economic evaluations of AI applications published between September 2019 and March 2025, following PRISMA and SWiM guidelines and registered in PROSPERO (CRD42025641230). Eligible studies were full economic evaluations comparing AI-based interventions with non-AI alternatives, and data were …

Rewriting The Monster: Carmilla As Laura's Gothic Double And Subversive Prince Charming In Le Fanu's Queer Gothic Fairy Tale, Annalee Tanner

English Theses

Rewriting the Monster: Carmilla as Laura's Gothic Double and Subversive Prince Charming in Le Fanu's Queer Gothic Fairy Tale

Diagnostic Stewardship Considerations In Infectious Diseases, Shawn Vasoo

Asian Medical Journal and Alternative Medicine

Diagnostic stewardship in infectious diseases is crucial to ensure that the right test is performed for the right patient for the right reasons at the right time, with the right interpretation which results in the right treatment, optimizing patient care. Managing clinicians have a critical role to play in thoughtful decision making in the pre- and post-analytic phases of test selection, interpretation and subsequent action. They also need to have a good understanding of the analytic phase of testing (test performance characteristics and limitations). In this paper, diagnostic stewardship across the main testing modalities for infectious diseases (culture, serology and …

Rsv In Pediatrics: Immunization Strategies And Treatment Insights, Mike Smith, Maria Deza Leon

Pediatrics in Practice: A CME Podcast

Respiratory Syncytial Virus (RSV) remains a leading cause of hospitalization in infants and young children. In this episode, we sit down with Dr. Maria Deza Leon to explore the latest in RSV prevention and treatment. From maternal immunization and monoclonal antibodies like Nirsevimab to practical guidance on managing severe cases, we’ll cover what pediatricians need to know this season. Tune in for evidence-based strategies, emerging therapies, and tips to navigate supply and coverage challenges—helping you protect your patients and stay ahead of RSV trends.

Click here to stream the podcast episode.

Mapping Climatic And Environmental Determinants Of Parasitic Helminth Prevalence In Southeast Asia, Angelica Avisado

UNLV Theses, Dissertations, Professional Papers, and Capstones

Climate change is reshaping environmental conditions across Southeast Asia (SEA) through rising temperatures, altered precipitation patterns, and increased droughts. These changes alter transmission dynamics of neglected tropical diseases (NTDs) by shifting spatial distribution, increasing larval development rates, faster pathogen growth development, and increasing transmission rates. NTDs are a group of preventable and treatable infections that disproportionately affect more than 1 billion people globally, specifically in tropical and subtropical regions. A subgroup of NTDs, parasitic helminth infections (PHIs) - particularly schistosomiasis, soil-transmitted helminths, and lymphatic filariasis remains a public health concern in the Southeast Asian region. PHIs and their ecological responses …

Results Of The 2024 Immunization Status Survey Of 24-Month-Old Children In Tennessee, Tennessee. Department Of Health.

Immunization Rates

No abstract provided.