Digital Commons Network^™

Improving Efficiency Of Prime Editing For Superior Genetic Traits In Livestock, Iuri Viótti Périssé

Funded Research Records

No abstract provided.

An Investigation Of Sex-Specific Effects Of Nucleus Accumbens Dopamine Receptor Signaling In Persistent Reward Seeking Under Punishment, Anna Caroline Toburen

Theses and Dissertations

Addiction involves persistent reward-seeking behavior despite adverse outcomes, with the nucleus accumbens (NAc) critically mediating motivation and cost-benefit decision-making. Dopamine (DA)-responsive neurons in the NAc, specifically D1 and D2 receptors within the NAc are crucial in encoding motivated behavior. However, the specific role of D1/D2 receptors in driving reward-seeking under punishment remains unclear. Preliminary findings suggest a sex-specific effect, with females exhibiting greater sensitivity to the suppressive effects of footshock on natural reward-seeking compared to males. We investigated the differential roles of NAc D1/D2 receptors in modulating reward-seeking behavior under punishment, with a focus on potential sex-specific effects. Using a …

Human Adipose Stem Cell-Derived Small Extracellular Vesicles Modulate Glial Cell Response In Aged And Young Tbi Mouse Models, Salma S. Abdelmaboud

USF Tampa Graduate Theses and Dissertations

Traumatic brain injury (TBI) is a leading cause of long-term neurological impairment, with aging significantly increasing vulnerability and worsening outcomes. Older individuals experience greater cognitive and motor deficits after TBI and often show diminished responses to therapeutic interventions. Both aging and TBI independently promote heightened neuroinflammation and cognitive dysfunction, increasing susceptibility to poor recovery. This study investigated the therapeutic potential of small extracellular vesicles (sEVs) derived from human adipose-derived stem cells (hASCs) to promote neurological recovery and modulate neuroinflammation in a mouse model of TBI. Male C57BL/6 mice aged 3, 15, and 20 months underwent controlled cortical impact (CCI) and …

Dissecting Microbial Communities With Single-Cell Transcriptome Analysis, Andrew W Pountain, Itai Yanai

Faculty, Staff and Student Publications

Revealing insights into the function of microbial communities requires moving beyond measuring bulk taxonomic composition to detecting interactions between subpopulations. Following the transformative impact of single-cell gene expression profiling techniques on numerous fields of human biology, recent years have seen increased application to microbes. We review progress in the development of these techniques and discuss challenges in applying them to microbial communities. We highlight applications for dissecting the microbiome in human health and disease that reveal functional heterogeneity within gut communities, antibiotic responses, and the dynamics of mobile genetic elements. As single-cell gene expression technologies continue to develop, they are …

Building Multimodal Knowledge Graphs: Automation For Enterprise Integration, Ritvik Garimella, Hong Yung Yip, Revathy Venkataramanan, Amit P. Sheth

Publications

As enterprises increasingly aim to incorporate artificial intelligence into their workflows to tackle complex, multimodal tasks, the demand for intelligent, robust, and trustworthy systems is paramount. While multimodal large language models offer initial capabilities for processing diverse data streams, their dependence on embedding-based representations limit their effectiveness in delivering semantically grounded explanations and reasoning, as well as qualities essential for enterprise-grade applications. Neurosymbolic approaches provide a promising alternative by enabling traceable, context-aware decision making. However, constructing enterprise-level multimodal knowledge graphs (MMKGs) that enable neurosymbolic approaches remains largely impractical. Although prior efforts have explored MMKG construction, they fall short in addressing …

Montana Kaimin, September 4, 2025, Students Of The University Of Montana, Missoula

Montana Kaimin, 1898-present

Student newspaper of the University of Montana, Missoula.

Controlling The Curriculum: A Content Analysis Of Prageru's Children's Educational Material, Heather Katherine Costa

Dissertations and Theses

PragerU is a conservative media outlet founded by Dennis Prager in 2009 that aims to insert new conservative content into existing U.S. public school curricula. While conservative activist groups have attempted for decades to alter educational content and policy, PragerU is attempting to directly influence public school curricula by having their materials authorized for use in public schools.

This study utilizes qualitative content analysis to understand the meaning behind PragerU's content, using a sample of 39 videos, targeted at students in grades 6 and higher. The transcript data were analyzed to address two research questions: 1) What are the key …

Lewy Body Dementia Promotion By Air Pollutants, Xiaodi Zhang, Haiqing Liu, Xiao Wu, Longgang Jia, Kundlik Gadhave, Lena Wang, Kevin Zhang, Hanyu Li, Rong Chen, Ramhari Kumbhar, Ning Wang, Chantelle E Terrillion, Bong Gu Kang, Bin Bai, Minhan Park, Ma Cristine Faye Denna, Shu Zhang, Wenqiang Zheng, Denghui Ye, Xiaoli Rong, Liu Yang, Lili Niu, Han Seok Ko, Weiyi Peng, Lingtao Jin, Mingyao Ying, Liana S Rosenthal, David W Nauen, Alex Pantelyat, Mahima Kaur, Kezia Irene, Liuhua Shi, Rahel Feleke, Sonia García-Ruiz, Mina Ryten, Valina L Dawson, Francesca Dominici, Rodney J Weber, Xuan Zhang, Pengfei Liu, Ted M Dawson, Shizhong Han, Xiaobo Mao

Faculty, Staff and Student Publications

Evidence links air pollution to dementia, yet its role in Lewy body dementia (LBD) remains unclear. Here we showed in a cohort of 56.5 million individuals across the U.S. that PM2.5 exposure raises LBD risk. Mechanistically, we found PM2.5 exposure led to brain atrophy in wild-type mice, an effect not seen in α-synuclein (αSyn)-deficient mice. PM2.5 exposure generated a highly pathogenic αSyn strain, PM-PFF, with enhanced proteinase K-resistance and neurotoxicity, resembling αSyn LBD strains. PM2.5 samples from China, the U.S., and Europe consistently induced proteinase-resistant αSyn strains and in vivo pathology. Transcriptomic analyses revealed shared responses between PM2.5-exposed mice and …

Air Pollution And Diseases: Signaling, G Protein-Coupled And Toll Like Receptors, Isabella Cattani-Cavalieri, Katrina F. Ostrom, Jordyn Margolis, Rennolds S. Ostrom, Martina Schmidt

Pharmacy Faculty Articles and Research

Air pollution is a significant public health issue that impacts lung health, particularly in vulnerable populations such as children, the elderly, and individuals with pre-existing respiratory conditions. Both natural and anthropogenic sources of air pollution give rise to a variety of toxic compounds, including particulate matter (PM), ozone (O₃), sulfur dioxide (SO₂), nitrogen dioxide (NO₂), carbon monoxide (CO), and polycyclic aromatic hydrocarbons (PAHs). Exposure to these pollutants is strongly associated with the development and exacerbation of respiratory diseases, including asthma, chronic obstructive pulmonary disease (COPD), lung cancer, and idiopathic pulmonary fibrosis (IPF). Notably, early life exposure to pollutants such as …

Effects Of Chatbot On Surgical Patients: A Systematic Review, Chung Man Ho, Mei Ping Priscilla Choi

International Nursing Research Congress (INRC)

Aim: To evaluate the effectiveness of chatbots in enhancing patient knowledge, alleviating psychological distress, and improving compliance compared to traditional methods among surgical patients.

Design: A systematic review.

Methods: Randomised controlled trials and quasi-experimental studies involving patients undergoing surgeries under general anaesthesia were reviewed. The risk of bias was assessed using the Cochrane Risk of Bias Tool for randomised controlled trials and the Risk of Bias in Non-randomised Studies of Interventions tool for non-randomised controlled trials.

Results: Nine studies with 1476 patients were included in this review. Conversational agents significantly improved perioperative knowledge, psychological distress and compliance with preoperative instructions, …

Blood Glucose Concentration In Highly Prolific Sows Before Farrowing: Its Relationship With Parity And Litter Size, Preechaphon Taechamaeteekul, Silvia Comencini, Fabio De Rensis, Roberta Saleri, Padet Tummaruk

The Thai Journal of Veterinary Medicine

Farrowing is an energy-demanding period for prolific sows, with blood glucose levels serving as key indicators affecting farrowing outcomes and piglet survival. This study aimed to evaluate blood glucose levels in prolific sows prior to farrowing, assess the prevalence of hypoglycemia in late gestation, and investigate the relationship between blood glucose levels, parity, and litter size under tropical conditions. A total of 67 Danish Landrace × Yorkshire sows were grouped based on litter size (6–14, 15–17, or ≥18 piglets) and parity (2–4 and 5–6). To determine glucose levels, blood samples were collected at 115.8 ± 1.6 days of gestation, at …

The Green Approach Of Arabic Gum-Based Adsorbent In Wastewater Treatment, Atheel H. Alwash

AUIQ Technical Engineering Science

The natural polysaccharide Arabic gum is a multifunctional, sustainable material used widely in wastewater treatment technology. Its active functional groups such as hydroxyl, carboxyl, and amino groups facilitate the efficient removal of heavy metals, dyes, pesticides, pharmaceuticals, and persistent organic pollutants. The high surface area and active functional groups facilitate its modification to form various structures such as hydrogels, nanocomposites, or biochar integrated into hybrid adsorption–photocatalysis systems. This review highlights the most familiar forms of Arabic gum in wastewater treatment technology, such as hydrogels, hydrogels nanocomposite, stabilizing agents, coating agents, and bio-activated carbon derived from Arabic gum using different preparation …

An Isoform-Specific Runx1c-Btg2 Axis Governs Aml Quiescence And Chemoresistance., Cuijuan Han, Zhiping Zhang, Edie I Crosse, Sogand Sajedi, Bin Lu, Xiyue Wang, Sadik Karma, Mitchell S. Kostich, Sakthi Harini Rajendran, Dylan B Udy, Steven Chen, Alexander Arnuk, Abimbola Eunice Lawal, Kayla R Koenig, Meryl Mckenna, Patrick K Reville, Hussein A Abbas, Omar Abdel-Wahab, Pedro Miura, Robert K Bradley, Eric Wang

Faculty Research 2025

Aberrant levels or structures of RNA isoforms are a hallmark of many cancers, including acute myeloid leukemia (AML), yet their role in AML chemoresistance remains unclear. We conducted a paired analysis of RNA isoform changes in patients with AML before therapy and at relapse after chemotherapy and identified intragenic DNA methylation at the proximal promoter of the transcription factor RUNX1, which resulted in elevated expression of the long-isoform RUNX1C through its alternative distal promoter. The unique N-terminal region of RUNX1C orchestrated an isoform-specific transcriptional program that promoted chemoresistance, with its direct target BTG2 playing a role in chemotherapy resistance. …

Life-History Stage-Dependent Effects Of Food Supplementation On Gut Microbiome Diversity In Sialia Sialis (Eastern Bluebird), Marcella D. Baiz, Allison Cornell

Biology Faculty Publications

Diet can be an important health factor that links fitness to an individual’s ecology. The gut microbiome responds to diet changes and plays an important role in host physiology. Dietary interventions are known to impact gut microbiome composition, but long-term effects and to what extent these impacts vary among individuals at different life-history stages are unclear. To address this question, we performed a supplemental feeding experiment by providing live mealworms (Tenebrio molitor) to Sialia sialis (Eastern Bluebird) during the chick-rearing period. We produced 16S metabarcoding data to quantify gut microbiome diversity from fecal samples of adults and nestlings …

In Re: Eileen Adams

2025 Decisions

USDC for the District of New Jersey

Commonwealth Times 2025-09-03

Commonwealth Times, 1969-

No abstract provided.

Left Atrial Thrombus In Rheumatic Mitral Stenosis: A Case Of Therapeutic International Normalized Ratio Failure, Muhammad Salman Sabri, Carly Weber, Yanis Belkadi, Donald C. Haas, Yasotha Rajeswaran

Division of Internal Medicine Faculty Papers & Presentations

BACKGROUND: Atrial fibrillation or flutter in rheumatic mitral stenosis (MS) increases left atrial appendage (LAA) thrombus risk despite therapeutic anticoagulation.

CASE SUMMARY: A 72-year-old woman on warfarin with moderate MS and atrial flutter presented with dyspnea. Transesophageal echocardiogram (TEE) showed a large LAA thrombus despite an international normalized ratio >2.5. Cardioversion was deferred, and the international normalized ratio target was raised to a range of 2.5 to 3.5.

TAKE-HOME MESSAGES: While cardioversion can be considered in patients with uninterrupted anticoagulation without TEE, those with rheumatic MS are at high risk of LAA and LA thrombus and should undergo TEE before …

September 3, 2025 Blazer Weekly, University Of Alabama At Birmingham

Blazer Weekly (student newsletter)

No abstract provided.

An Allele-Agnostic Mutant-Kras Inhibitor Suppresses Tumor Maintenance Signals And Reprograms Tumor Immunity In Pancreatic Cancer, Kathleen M Mcandrews, Francesca Paradiso, Clint A Stalnecker, Benson S Chellakkan, Fredrik I Thege, David H Peng, Barbara A Moreno Diaz, Hikaru Sugimoto, Sarah I Patel, Krishnan K Mahadevan, Michelle L Kirtley, Danielle Wills, Amari M Sockwell, Andre Luis F Fonseca, Yunhe Liu, Kimal I Rajapakshe, Nathaniel G Yee, Phuong Thao Tran, Huda Alchikh Omar, Antonio Tedeschi, Fiorella Schischlik-Siegl, Andrew S Boghossian, Matthew G Rees, Melissa M Ronan, Jennifer A Roth, Dorothea Rudolph, Martin Aichinger, Florian Ebner, Artem V Artemov, Jesse Lipp, Laura Pisarsky, Valerie Laura Herrmann, John Park, Jörg F Rippmann, Otmar Schaaf, Vanessa Chandler, Mariah Williams, Charles E Deckard, Linghua Wang, Channing J Der, Christopher Vellano, Paola A Guerrero, Timothy P Heffernan, Raghu Kalluri, Anirban Maitra

Faculty, Staff and Student Publications

KRAS is among the most frequently mutated oncogenes in cancer, and for decades, efforts at pharmacological blockade of its function in solid cancers have been unsuccessful. A notable advance in this endeavor is the recent development of small molecule KRAS inhibitors, which enable direct targeting of the mutant oncoprotein. Here, we comprehensively evaluate the pre-clinical efficacy of BI-2493 a panKRASi, a first-in-class allele agnostic mutant KRAS inhibitor, in pancreatic ductal adenocarcinoma (PDAC). We report effective tumor growth suppression across a broad range of models, including cell lines, patient-derived xenografts (PDXs), syngeneic orthotopic models, and prolonged survival in genetically engineered mouse …

The Parthenon, September 03, 2025, Baylee Parsons, Kaitlyn Fleming, Ashton Pack, Nate Harrah, Caden Adkins, Scott Price, Lilli Ross, Wade Sullivan

The Parthenon

No abstract provided.

Genome-Wide Association Study And Genomic Prediction Of Leaf Spot (Stemphylium Vesicarium) Resistance In Spinach Diversity Panel, Gehendra Bhattarai, Bo Liu, James Cor, Ainong Shi

Horticulture Faculty Publications and Presentations

Stemphylium leaf spot (SLP), caused by Stemphylium vesicarium, has emerged as an increasing threat to spinach production in the United States, with widespread outbreaks reported across major spinach-growing regions over the past two decades. The objectives of this study were to: (1) evaluate global USDA spinach germplasm collections and commercial cultivars for resistance to S. vesicarium; (2) perform genome-wide association studies (GWAS) to identify genomic regions associated with resistance; and (3) conduct genomic prediction (GP) to enhance selection accuracy. A total of 311 diverse spinach genotypes, including USDA germplasm accessions and commercial cultivars, were evaluated under greenhouse conditions at the …

An Isoform-Specific Runx1c-Btg2 Axis Governs Aml Quiescence And Chemoresistance, Cuijuan Han, Zhiping Zhang, Edie I Crosse, Sogand Sajedi, Bin Lu, Xiyue Wang, Sadik Karma, Mitch Kostich, Sakthi Harini Rajendran, Dylan B Udy, Steven Chen, Alexander Arnuk, Abimbola Eunice Lawal, Kayla R Koenig, Meryl Mckenna, Patrick K Reville, Hussein A Abbas, Omar Abdel-Wahab, Pedro Miura, Robert K Bradley, Eric Wang

Faculty, Staff and Student Publications

Aberrant levels or structures of RNA isoforms are a hallmark of many cancers, including acute myeloid leukemia (AML), yet their role in AML chemoresistance remains unclear. We conducted a paired analysis of RNA isoform changes in patients with AML before therapy and at relapse after chemotherapy and identified intragenic DNA methylation at the proximal promoter of the transcription factor RUNX1, which resulted in elevated expression of the long-isoform RUNX1C through its alternative distal promoter. The unique N-terminal region of RUNX1C orchestrated an isoform-specific transcriptional program that promoted chemoresistance, with its direct target BTG2 playing a role in chemotherapy resistance. …

Chapter 1: The Ink Spell, Garon C. Smith

G. Wiz’s Book of Spells

The Ink Spell demonstration is a dramatic way to engage audiences into the wonders of chemical reactions. Two potions are swirled together in an Erlenmeyer flask as an incantation is delivered. As the spell is concluded, the liquid instantly turns from clear to purplish black when the wizard’s hand rubs the bottom of the flask. It is actually the well-known iodine clock reaction dressed up as the opening act of a chemistry course or a wizard show. It is a great example of chemical kinetics, allowing chances for demonstrating the effect of concentrations and temperature on the rate of the …

Persistent Na+ Current Couples Spreading Depolarization To Seizures In Scn8a Gain-Of-Function Mice, Isamu Aiba, Yao Ning, Jeffrey L Noebels

Faculty, Staff and Students Publications

Spreading depolarization (SD) is a slowly propagating wave of massive cellular depolarization that transiently impairs the function of affected brain regions. Although SD typically arises as an isolated hemispheric event, we previously reported that reducing M-type potassium current (IKM) by ablation of Kcnq2 in forebrain excitatory neurons results in tightly coupled spontaneous bilateral seizure–SD complexes in the awake mouse cortex.

Here we find that enhanced persistent Na+ current attributable to gain-of-function mutations in Scn8a (N1768D/+, hereafter D/+) produces a similar compound cortical excitability phenotype. Chronic direct-current (DC)-band EEG recording detected spontaneous bilateral seizure–SD complexes accompanied by seizures with …

Nebulization Of An Mrna-Encoded Monoclonal Antibody For Passive Immunization Of Foals Against Rhodococcus Equi, Rebecca M Legere, Jeannine A Ott, Cristina Poveda, Daryll Vanover, Karin E R Borba, Jae Yeon Joo, Cameron L Martin, Bibiana P Da Silveira, Jocelyne M Bray, Kerstin Landrock, Gus A Wright, J Chistensen Blazier, Andrew E Hillhouse, Ashley L Benham-Duret, Brandon Mistretta, Rafaela L Klein, Sarah M Thompson, Amelia R Woolums, Michael F Criscitiello, Luc R Berghman, Angela I Bordin, Philip J Santangelo, Jeroen Pollet, Noah D Cohen

Faculty, Staff and Students Publications

Inhalation of Rhodococcus equi causes severe pneumonia in humans and animals worldwide, most commonly affecting horse foals. The standard for preventing R. equi pneumonia in foals is transfusion of hyperimmune plasma, which is expensive and carries the risk of adverse effects. Our goal was to passively immunize foals against R. equi by nebulizing mRNA encoding an equine monoclonal antibody (mAb) against the virulence-associated protein A (VapA) directly into the lungs. VapA-specific memory B cells from an immunized horse were used to identify and select the sequence for an equine immunoglobulin (Ig)G1 mAb. In vitro-transcribed mRNA encoding this sequence expressed …

The Sea Ice For Walrus Outlook—A Network For Weather And Sea-Ice Information Sharing In The Bering Strait, Alaska, Lisa Sheffield Guy

Benefits of Ocean Observing Catalog (BOOC)

The Sea Ice for Walrus Outlook (SIWO) is a collaborative information-sharing network that delivers weekly springtime updates on sea ice and weather conditions relevant to walrus habitat in the Bering Strait region of Alaska. Launched in 2010, SIWO shares Indigenous Knowledge from Alaska Native sea-ice experts alongside custom forecasts and imagery from the National Weather Service Alaska, the University of Alaska Fairbanks, and the Alaska Ocean Observing System. Weekly reports incorporate community-based observations, satellite imagery, 7-day forecasts, and animated current and ice movement models to inform subsistence hunters and support maritime safety. This hybrid approach facilitates real-time communication and mutual …

Use Of Eagala Model–Based Psychotherapy For Youth Psychological Disorders, Kimberley D. Ryan, Tammy L.B. Mckenzie

People and Animals: The International Journal of Research and Practice

Global youth psychological disorder prevalence has increased exponentially. Therefore, a drastic increase in mental health care utilization is expected. Since traditional approaches to the treatment of adult psychological disorders are ineffective with youth, alternative approaches to treatment of youth psychological disorders must be considered. One such approach is equine assisted psychotherapy (EAP), a person-centered, experiential approach using horses during the treatment of psychological disorders and the basis of the Equine Assisted Growth and Learning Association (Eagala) model–based programming. This article presents the quantitative research findings of a study investigating the effectiveness of the Eagala model–based EAP programming in the treatment …

Introducing Therapy Dog Programs Within Organizational Settings: A Scoping Review Of Implementation Processes And Practices, Laura Mundy, Carlie Driscoll, Janette Young

People and Animals: The International Journal of Research and Practice

Organizations are introducing therapy dog programs at a growing rate. Standards and guidelines for including therapy dogs in programs with people are also being developed and made available to those implementing such programs. How organizations are implementing programs, how they are aligning with available standards, and the presence of any barriers and facilitators to their implementation has been largely unexplored. Therefore, the aim of our review was to summarize the literature available on the implementation of therapy dog programs within organizational settings. Furthermore, we aimed to summarize available descriptions of program practices and their alignment with best practice guidelines, and …

Comparison Of Hepcidin-25 And Angiogenin (Rnase-5) Levels In Healthy Children And Children With Brucellosis, Ümmü Seleme Mumcu, Özge Metin Akcan, Mehmet Özdemir, Gökçe Kader Arslan, Mustafa Gençeli

Journal of Pediatric Infectious Diseases

Objective: The aim of this study is to determine whether there is a change in serum levels of the anti-microbial peptides Hepcidin-25 and angiogenin, in pediatric patients with Brucellosis compared to healthy controls. In addition, to evaluate their role in brucellosis and relationship with complications of brucellosis. Methods: In our study, children aged 3 to 18 years were included. There were 50 patients in the brucellosis group and 40 healthy children in the control group. Serum hepcidin-25 and angiogenin levels were studied, as well as complete blood count, alanine aminotransferase, aspartate aminotransferas, C-reactive protein, erythrocyte sedimentation rate. The relationship between …

Complete Issue - Volume 87, No. 1 And 2, Jere Nash, Jim Gulley, J. Janice Coleman, Terry Beckenbaugh, Megan Bodily, William S. Critchfield, Nathan Drake, Charles E. Jones, William Harrison Taylor, Emma L. Waldie

Journal of Mississippi History

No abstract provided.