Digital Commons Network^™

Structure Of An Lgr Dimer, An Evolutionary Predecessor Of Glycoprotein Hormone Receptors, Zhen Gong, Shuobing Chen, Ziao Fu, Brian Kloss, Chi Wang, Jonathan Kim, Oliver B Clarke, Qing R Fan, Wayne A Hendrickson

2020-Current year OA Pubs

Glycoprotein hormones (GpHs) produced in the human pituitary act through receptors (GpHRs) in the gonads to support reproduction and in the thyroid for metabolism. GpHs are heterodimeric cystine-knot proteins; their receptors bind cognate hormones at an extracellular domain and signal through a transmembrane domain to heterotrimeric G proteins. GpHs and GpHRs have co-evolved from invertebrate counterparts. Structures of the human receptors as isolated for cryogenic electron microscopy (cryo-EM) are all monomeric despite compelling evidence for their functioning as dimers. Here we characterize the homologous receptor from Caenorhabditis elegans. Its biochemical properties are notably similar to those of the thyroid stimulating …

Development Of Intestinal Tissue-Resident T Cells In Early-Life Mice Before And After Weaning, Catherine Montenegro, Siyuan Ding, Federico Perdomo-Celis, Manuel A Franco

2020-Current year OA Pubs

Intestinal tissue-resident T cells have been poorly characterized during early-life. Here, we phenotypically characterized tissue-resident (intravascular staining negative) CD4

Crispr Disruption Of Scarna1 Reduces Pseudouridylation In Spliceosomal Rna U2 At U89 And Perturbs The Transcriptome In Hek293t Cells., Amanda Gardner-Kay, Lynndy Le, Michael Filla, Nataliya Kibiryeva, James O'Brien, Douglas C. Bittel

Manuscripts, Articles, Book Chapters and Other Papers

Small Cajal body-associated RNAs (scaRNAs) are essential for biochemical modification of spliceosomal RNAs and spliceosome function. Changes in scaRNA expression level have been associated with developmental issues, including cancer and congenital heart defects (CHDs), although the mechanism remains unclear. Small Cajal body-associated RNA 1 (scaRNA1) guides pseudouridylation at uridine 89 (Ψ89) of the spliceosomal RNA U2, a highly conserved modification that may be critical for spliceosome function. To investigate the role of scaRNA1 in splicing regulation, CRISPR-Cas9 genome editing was used to introduce targeted deletions in the scaRNA1 locus in HEK293T cells. Edited clones were identified by T7 endonuclease I …

Microrna Profiling Identifies Diagnostic And Prognostic Markers In Pediatric Sarcoma., Terrie Flatt, Leonid M. Yermakov, Shreeram Akilesh, Eleanor Y. Chen, Elizabeth Gonzalez, Alejandro Parrales, Marta Zapata-Tarres, Rocio Cardenas-Cardos, Liliana Velasco-Hidalgo, Celso Corcuera-Delgado, Rodolfo Rodriguez-Jurado, Lillia García-Rodríguez, Midhat S. Farooqi, Atif Ali Ahmed

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: MicroRNAs (miRNAs) function as post-transcriptional gene expression regulators and influence the development and progression of several cancers, yet their roles in pediatric sarcomas remain poorly defined.

METHODS: RNA extracted from formalin-fixed paraffin-embedded tumor tissue scrolls of 108 pediatric tumors, including 32 osteosarcoma (OS), 26 Ewing's sarcoma (EWS), and 50 rhabdomyosarcoma (RMS) cases, were analyzed for microRNA expression using the NanoString multiplex nCounter platform that yielded information on 827 human miRNAs. The expression of candidate miRNAs was validated with in situ hybridization (miRNA-ISH) and QuPath quantification on tissue microarray slides comprising an independent set of 48 OS, 17 EWS, and …

Digital Neuropsychology And Social Anxiety: The Effect Of Observer Presence And Personality On Stress And Cognition, Ava M. Dixon

Master's Theses

This study examined how observer presence and social anxiety influence stress and cognitive performance during neuropsychological assessments, focusing on the potential benefits of unsupervised digital testing environments. Eighty university students were randomly assigned to observed or unobserved digital neuropsychological conditions and completed assessments of social cognition and executive functioning and questionnaires of social anxiety and personality. Participants also completed measures of state anxiety and heart rate variability at baseline and after cognitive tests. The findings indicated that observation led to higher subjective anxiety, but it did not produce significant interactions with social anxiety on stress or cognitive performance. Post-hoc analyses …

Non-Invasive Ultrasonic Neuromodulation Of The Human Nucleus Accumbens Impacts Reward Sensitivity, Siti N Yaakub, John Eraifej, Nadège Bault, Mathilde Lojkiewiez, Elouan Bellec, Jamie Roberts, Noah S Philip, Amir Puyan Divanbeighi Zand, Alexander L Green, Matthew F S Rushworth, Elsa F Fouragnan

School of Psychology

Precisely neuromodulating deep brain regions could bring transformative advancements in both neuroscience and treatment. We demonstrate that non-invasive transcranial ultrasound stimulation (TUS) can selectively modulate deep brain activity and affect learning and decision making, comparable to deep brain stimulation (DBS). We tested whether TUS could causally influence neural and behavioural responses by targeting the nucleus accumbens (NAcc) using a reinforcement learning task. Twenty-six healthy adults completed a within-subject TUS-fMRI experiment with three conditions: TUS to the NAcc, dorsal anterior cingulate cortex (dACC), or Sham. After TUS, participants performed a probabilistic learning task during fMRI. TUS-NAcc altered BOLD responses to reward …

How Human Personality Will Change With The Use Of Artificial Intelligence, John Mayer

UNH Personality Lab

INTRODUCTION: Human personality changes in response to new habits, situations, and maturation, among other causes. As people interact with artificial intelligence (AI), their personalities are likely to change, from their emotional responses to their self-concept.

METHODOLOGY: This theoretical account draws together empirical studies of how personality changed in response to past technological innovations, changes in personal interactions observed in people’s AI usage, changes that arise from the use and disuse of cognitive skills, and works on the self-concept. Research reports in the review were selected according to their relevance and quality.

THEORETICAL STATEMENT: While AI becomes increasingly human-like, humans increasingly …

Red Midge Larvae Are An Invertebrate Alternative Diet To Beef Liver For Planarian Husbandry, Jonah Pacis , '25, Danielle Ireland, Evangeline Coffinas, Jerome Sheehan, Kate Sun , '27, Eva-Maria S. Collins

Biology Faculty Works

Freshwater planarians are an emerging model for toxicology and neuroscience because of their amenability to rapid behavioral screening and remarkable ability to regenerate a cephalized nervous system. As invertebrates, planarians can help reduce the use of vertebrates in research; however, laboratories typically maintain planarians on diets of homogenized organic beef or chicken liver, raising ethical concerns with feeding a vertebrate diet. Organic liver is difficult to obtain, and preparation methods vary, introducing intra- and interlaboratory variability. Here, we show that Dugesia japonica planarians can be maintained for over a year on commercially available red midge larvae (RML), a natural prey …

The Mechanism Of Action Of Remote Electrical Neuromodulation (Ren) In Treating Migraine And Potentially Other Idiopathic Pain Conditions, Marius Birlea, Christopher L. Robinson, Eden Mama, Alit Stark-Inbar, Alon Ironi, Daniel Atashsokhan, Thomas Berk

College of Nursing Faculty Papers & Presentations

Purpose of Review

This review provides evidence for the mechanism of action (MOA) of Remote Electrical Neuromodulation (REN), a non-pharmacological therapeutic technology for treating migraine and other symptoms manifested by certain idiopathic pain diseases. It discusses the main physiological and anatomical aspects associated with conditioned pain modulation (CPM), and how REN is applied for the treatment of migraine pain and other symptoms.

Recent Findings

The REN wearable device (Nerivio®) is FDA cleared for acute and preventive treatment of migraine in patients aged 8 years and above, and CE marked in patients aged 12 and above. In brief, the REN MOA …

‘The Truth Was, Most Biodiversity Was Redundant’: Caring About Mass Extinction In Ned Beauman’S Venomous Lumpsucker, David Sergeant

School of Law, Humanities and Social Sciences

This essay reads Ned Beauman’s Venomous Lumpsucker as exploring the gap that exists between caring about mass species extinction and taking meaningful action to prevent it. The novel, at first, undermines the conventional reasons for caring; however, one of its protagonists is then revealed as having become willing to die for the issue. Equally, the reason for this change proves strangely hard to locate. This essay argues that the real reason for caring about species extinction in Venomous Lumpsucker is connected to a transcendence of the self that aligns the novel with both science fiction and spiritual traditions; however, this …

The 23rd International Medical, Pharmaceutical, Cosmeceutical And Health Science Symposium (Impachs), Sultan Qaboos University

Sultan Qaboos University Medical Journal

No abstract provided.

Apocalypticism And Prognostication In Early ShiʿI Literature, Mohammad Amin Mansouri

All Faculty Scholarship for the College of Arts and Humanities

This article studies the origins of Jafr, an apocalyptic, eschatological and occult book attributed to the first Shiʿi imam, ʿAlī ibn Abī Ṭālib (d. 661). While it remains unclear whether Jafr was ever physically composed, it became associated with lettrism (ʿilm al-ḥurūf) in medieval Sunni and Shiʿi literature. Jafr gradually evolved into a crucial component of Islamic occult traditions and influenced various cosmological theories as well as the letter-magic practices of prominent Sunni and Shiʿi occultists. Despite its historical significance, confusion regarding Jafr’s roots, authorship and content in Shiʿi sources from the third to fifth centuries AH …

Crispr Disruption Of Scarna1 Reduces Pseudouridylation In Spliceosomal Rna U2 At U89 And Perturbs The Transcriptome In Hek293t Cells, Amanda Gardner-Kay, Lynndy Le, Michael Filla, Nataliya Kibiryeva, James E. O'Brien Jr., Douglas C. Bittel

Student Publications

Small Cajal body-associated RNAs (scaRNAs) are essential for biochemical modification of spliceosomal RNAs and spliceosome function. Changes in scaRNA expression level have been associated with developmental issues, including cancer and congenital heart defects (CHDs), although the mechanism remains unclear. Small Cajal body-associated RNA 1 (scaRNA1) guides pseudouridylation at uridine 89 (Ψ89) of the spliceosomal RNA U2, a highly conserved modification that may be critical for spliceosome function. To investigate the role of scaRNA1 in splicing regulation, CRISPR-Cas9 genome editing was used to introduce targeted deletions in the scaRNA1 locus in HEK293T cells. Edited clones were identified by T7 endonuclease I …

Transdisciplinary Perspectives On Ai: The Fourth Annual Conference Of The European Culture And Technology Laboratory, Connell Vaughan, Ioana Madalina Moldovan, Silivan Moldovan, Noel Fitzpatrick

Books/Book Chapters

The fourth annual conference of the ECT Lab+ was hosted by Technical University of Cluj-Napoca over two days in October 2024 at the Cluj Innovation Park. The conference brought together experts from the Arts, Humanities, Social Sciences, Technology, and other fields to discuss and reflect on the advent of Artificial Intelligence and how the associated technologies are transforming how we live, work and study. Under the title Transdisciplinary perspectives on AI: Alternative Histories, Current Practices and Possible Futures the conference moved beyond simplistic technophila and technophobia to consider whether we can co-evolve with these new technologies which combine machine learning …

The Gastrointestinal Endothelin System: Receptors, Cellular Actions, And Involvement In Gastrointestinal Disorders, Arjun Singh, Rajvir Singh

Department of Medicine Faculty Papers

The gastrointestinal endothelin system regulates vital processes including motility, secretion, sensation, and inflammation. Endothelin-1 acts via endothelin A and B receptors expressed on diverse intestinal cells. Dysregulation of endothelin signaling underlies common gastrointestinal disorders such as inflammatory bowel disease and irritable bowel syndrome. Aberrant endothelin-1 production and endothelin A receptor activation contribute to the inflammation, pain hypersensitivity, impaired epithelial function, and smooth muscle hypercontractility characteristic of these diseases. Endothelin receptor antagonists may provide therapies for managing related symptoms. However, knowledge gaps exist regarding cell-specific signaling pathways, regulatory factors, and precise roles in intestinal pathologies. Elucidating these details and translating insights …

Human Plasma Proteomic Profile Of Clonal Hematopoiesis, Zhi Yu, Amélie Vromman, Ngoc Quynh H Nguyen, Art Schuermans, Linke Li, Thiago Rentz, Tetsushi Nakao, Shamsudheen K Vellarikkal, Md Mesbah Uddin, Abhishek Niroula, Gabriel Griffin, Michael C Honigberg, Amy E Lin, Christopher J Gibson, Daniel H Katz, Usman A Tahir, Shi Fang, Jacqueline S Dron, Michael Pan, Sara Haidermota, Shriienidhie Ganesh, Tajmara Antoine, Joshua Weinstock, Thomas R Austin, Ramachandran S Vasan, Gina M Peloso, Whitney Hornsby, Peter Ganz, Joann E Manson, Bernhard Haring, Charles Kooperberg, Alexander P Reiner, Joshua C Bis, Bruce M Psaty, Yuan-I Min, Adolfo Correa, Leslie A Lange, Wendy S Post, Jerome I Rotter, Stephen S Rich, James G Wilson, Benjamin L Ebert, Bing Yu, Christie M Ballantyne, Josef Coresh, Vijay G Sankaran, Alexander G Bick, Siddhartha Jaiswal, Robert E Gerszten, Nhlbi Trans-Omics For Precision Medicine, Peter Libby, Rajat M Gupta, Pradeep Natarajan

Faculty, Staff and Student Publications

Plasma proteomic profiles associated with subclinical somatic mutations in blood cells may offer insights into downstream clinical consequences. Here we explore these patterns in clonal hematopoiesis of indeterminate potential (CHIP), which is linked to several cancer and non-cancer outcomes, including coronary artery disease (CAD). Among 61,833 participants (3881 with CHIP) from TOPMed and UK Biobank (UKB) with blood-based DNA sequencing and proteomic measurements (1,148 proteins by SomaScan in TOPMed and 2917 proteins by Olink in UKB), we identify 32 and 345 proteins from TOPMed and UKB, respectively, associated with CHIP and most prevalent driver genes (DNMT3A, TET2, and ASXL1). These …

Knowledge, Attitude And Practice Of Butchers Regarding Crimean Congo Hemorrhagic Fever In An Endemic Area Of Iran, Mohammad Amin Gorouhi, Abbas Aghaei Afshar, Najmeh Mansoori, Parya Jangipour Afshar, Leila Shirani-Bidabadi, Ismaeil Alizadeh, Nariman Shahhosseini

Chulalongkorn Medical Journal

Background: In endemic countries, Crimean-Congo hemorrhagic fever (CCHF) is an important zoonotic disease with significant public health implications. Previous studies in Iran suggests that CCHF poses a significant health risk to slaughterhouse workers and butchers. The main objecive of this study was to investigate the knowledge, attitude, and practice of butchers regarding CCHF at their workplace in an endemic area in southeastern Iran.

Methods: A descriptive cross-sectional study was conducted from January to March 2022 in Zarand city. In total, 60 butchers were included in the city. Statistical analysis was performed using SPSS version 22.

Results: The average scores of …

Trauma Therapy For Orcs: A Psychotherapeutic Consideration Of Orcs In Tolkien's Legendarium, Karen L. Pollock Ms

Journal of Tolkien Research

The complex, violent behavior of the Orcs can be understood through the lens of modern trauma psychology, specifically Complex Post-Traumatic Stress Disorder (CPTSD) and the mechanism of Intergenerational Trauma Transmission.

CPTSD is a diagnosis often applied to individuals who have experienced prolonged, repeated, and inescapable trauma, particularly within an abusive or authoritarian environment. The foundational myth of the Orcs—that they were captured, enslaved, tortured, and physically and mentally mutated by Morgoth—perfectly aligns with a foundational, systemic trauma event.

The resulting Orcish behaviors align with the core symptom clusters of CPTSD including; emotional dysregulation,dissociation/distorted self-perception, disturbed relationships, and a lack of …

How Not To Go Viral? A Covid-19 Handbook For Students

University press releases and calendars

No abstract provided.

Biochar Derived From Different Sources Ameliorates The Phytochemical And Physiological Characteristics Of Foxtail Millet (Setaria Italica) Under Water Stress, Mohammad Keshavarz, Ehsan Bijanzadeh, Hamid Reza Boostani

Turkish Journal of Botany

Organic material, such as biochar, can improve soil fertility and crop yield under water stress. A pot experiment was conducted to consider the effects of biochar on the pigment content, enzyme activity, physiological characteristics, and nutrient uptake of foxtail millet under water stress. Plants were tested under normal, mild, and severe irrigation regimes (100%, 75%, and 50% field capacity, respectively) using different biochar sources (licorice root biochar (LRB), cow manure biochar (CMB), cotton residue biochar (CRB), and municipal waste biochar (MWB)) and a control without fertilizer. Under severe water stress, the chlorophyll a content and total chlorophyll increased by 137.3% …

Tannin Supplementation Alters Foraging Behavior And Spatial Distribution In Beef Cattle, Bashiri Iddy Muzzo, R. Douglas Ramsey, Kelvyn Bladen, Juan J. Villalba

Wildland Resources Student Research

Beef production on chemically uniform grass monocultures can limit nutrient synchrony and contribute to uneven pasture use. We evaluated whether supplementing tannins with bioactive plant secondary compounds improves foraging dynamics and landscape use by beef cattle grazing a meadow bromegrass monoculture in ways aligned with rangeland sustainability. Twenty-four Angus cow–calf pairs were allocated to six 3.6-ha paddocks (four pairs/paddock), randomly assigned to Control (Ctrl; n = 3) or Tannin treatment (TT; n = 3). Animals received 1 kg/cow/day of DDGs, with TT receiving an added 0.4% tannins (2:1 condensed:hydrolyzable). Grazing occurred during four 15-day periods (July– September) across two years. …

Art Teams Year 4 Annual (Ad Hoc) Report 2024-2025, Guy Trainin, Kimberley D'Adamo, Hyeonjin Yoon, Azadeh Hassani, Zohreh Tamimdari, Jiabin Lyu, Michael Nti Ababio, Sarah Trainin

Research and Evaluation in Education, Technology, Art, and Design

This report documents the fourth year of the Art TEAMS project, a multi-year professional learning initiative focused on arts-integrated, transdisciplinary teaching practices across Nebraska K-12 schools. During the 2024-2025 academic year, the project advanced work with its second cohort of educators while maintaining engagement with the first cohort to assess the sustainability of implementation. The second cohort brought increased diversity in art forms, including ceramics, digital media, and career and technical education, demonstrating the versatility of arts integration across curricula.

Year Four activities included delivering refined professional development courses grounded in emergent curriculum design, with significant curriculum modifications based on …

Dna Extrusion Size Determines Pathway Choice During Cag Repeat Expansion, Mayuri Bhatia, Ashutosh S. Phadte, Anna Lakhina, Anthony R. Monte Carloi Iii, Sarah Barndt, Anna Pluciennik

Department of Biochemistry and Molecular Biology Faculty Papers

DNA triplet repeat expansion causes several primarly neurological disorders like Huntington's disease, myotonic dystrophy type 1, and fragile-X related disorders. There is general consensus that recognition of extrahelical extrusions or hairpin-loop structures (formed by strand slippage) by the DNA mismatch repair protein MutSβ leads to repeat expansion by a mutagenic process. By contrast, the FAN1 nuclease attenuates triplet repeat expansion, the molecular basis of which was explained by our recent finding that FAN1 nuclease cleaves and initiates removal of extrahelical extrusions. Here we show that extrusions containing two or more triplet repeats are subject to recognition and processing by either …

Educational Program For Primary Care On Reducing Antibiotic Overuse For Upper Respiratory Infections, Adebusola Onwuemene

Biennial Convention (CONV)

With the advent of antibiotics in 1928, the death rate declined (Fleming, 1980; Hoyert et al., 1999). However, antibiotics’ effectiveness led to the notion that antibiotics could be used for various illnesses. Thus, the overuse of antibiotics leaped, causing antibiotic resistance, rising healthcare costs, and increased mortality rates (CDC, 2024). Many research studies identified factors influencing antibiotic overuse for upper respiratory infections (URIs) in primary care, such as primary care providers’ ignorance, and patients’ expectations (Carlsson et al. , 2023; Gidengil et al., 2016). This study aimed to provide an educational program, via the Canva media tool, on reducing the …

Exploring Experiences Of Mental Health Challenges In Under-Represented Young People (Aged 16–24 Years) In England: A Narrative Inquiry Protocol, Rebecca Syed Sheriff, Jason Arday, Rohit Shankar, Roisin Mooney, Louise Chandler, Helen Adams, Lili Z Nagy, Roger Farrell, Daisy Fancourt, Scott Weich, Catherine Henderson, Shaima Hassan, Joe Langley, Kamaldeep Bhui

Peninsula Medical School

Introduction Three-quarters of mental health problems start before the age of 25. However, young people are the least likely to receive mental healthcare. Some young people (such as those from ethnic minorities) are even less likely to receive mental healthcare than others. Long-term impacts of mental health problems include poorer physical health, relationships, education and employment. We aim to elicit the views, experiences and needs of diverse young people (aged 16–24 years), to better understand (1) their experiences of under-representation, mental health and coping, (2) mechanisms that shape mental health trajectories and (3) how online arts and culture might be …

Reclaiming Relational Pedagogies: The Role Of Talking Circles In Decolonizing Higher Education Classrooms, Veneice Lacy

Faculty Research, Scholarly, and Creative Activity

This article explores the use of Talking Circles as a decolonizing pedagogical practice within higher education, grounded in Indigenous epistemologies and relational worldviews. Drawing on the works of Indigenous scholars such as Graveline, Wilson, Archibald, and Simpson, the article positions Talking Circles not merely as instructional strategies but as ceremonial, spirit-centered pedagogies that resist the Eurocentric hierarchical and individualistic norms of Western academia. Through autoethnographic reflection and student narratives from undergraduate and graduate education courses, the article demonstrates how Talking Circles foster relational accountability, ethical engagement, and transformative learning. By centering oral tradition, reciprocity, and communal learning, Talking Circles reconfigure …

Investigating The Neuronal Role Of The Proteasomal Atpase Subunit Gene Psmc5 In Neurodevelopmental Proteasomopathies, Sébastien Küry, Janelle E Stanton, Geeske M Van Woerden, Amélie Bosc-Rosati, Tzung-Chien Hsieh, Lise Bray, Marielle Oloudé, Cory Rosenfelt, Marie Pier Scott-Boyer, Victoria Most, Tianyun Wang, Jonas J Papendorf, Charlotte De Konink, Wallid Deb, Virginie Vignard, Maja Studencka-Turski, Thomas Besnard, Anna M Hajdukowicz, Franziska G Thiel, Sophie Wolfgramm, Laëtitia Florenceau, Silvestre Cuinat, Sylvain Marsac, Yann Verrès, Audrey Dangoumau, Léa Poirier, Ingrid M Wentzensen, Annabelle Tuttle, Cara Forster, Johanna Striesow, Richard Golnik, Damara Ortiz, Laura Jenkins, Jill A Rosenfeld, Alban Ziegler, Clara Houdayer, Dominique Bonneau, Erin Torti, Amber Begtrup, Kristin G Monaghan, Sureni V Mullegama, Catharina M L Nienke Volker-Touw, Koen L I Van Gassen, Renske Oegema, Mirjam S De Pagter, Katharina Steindl, Anita Rauch, Ivan Ivanovski, Kimberly Mcdonald, Emily Boothe, Andrew Dauber, Janice Baker, Noelle Andrea V Fabie, Raphael A Bernier, Tychele N Turner, Siddharth Srivastava, Kira A Dies, Lindsay C Swanson, Carrie Costin, Alali Abdulrazak, Rebekah K Jobling, John Pappas, Rachel Rabin, Dmitriy Niyazov, Anne Chun-Hui Tsai, Karen Kovak, David B Beck, May Christine V Malicdan, David R Adams, Lynne Wolfe, Rebecca D Ganetzky, Colleen C Muraresku, Davit Babikyan, Zdeněk Sedláček, Miroslava Hančárová, Andrew T Timberlake, Hind Al Saif, Berkley Nestler, Kayla King, M J Hajianpour, Gregory Costain, D'Arcy Prendergast, Chumei Li, David Geneviève, Antonio Vitobello, Arthur Sorlin, Christophe Philippe, Tamar Harel, Ori Toker, Ataf Sabir, Derek Lim, Mark J Hamilton, Lisa J Bryson, Elaine Cleary, Sacha Weber, Trevor L Hoffman, Anna M Cueto-González, Eduardo F Tizzano, David Gómez-Andrés, Marta Codina-Solà, Athina Ververi, Efterpi Pavlidou, Alexandros Lambropoulos, Kyriakos Garganis, Marlène Rio, Jonathan Levy, Sarah J Langas, Anne M Mcrae, Mathieu K Lessard, Maria Daniela D'Agostino, Isabelle De Bie, Meret Wegler, Rami Abou Jamra, Susanne B Kamphausen, Viktoria Bothe, Lorraine Potocki, Eric Olinger, Yves Sznajer, Elsa Wiame, Michelle L Thompson, Molly C Schroeder, Catherine Gooch, Raphael A Smith, Arti Pandya, Larissa M Busch, Uwe Völker, Elke Hammer, Kristian Wende, Benjamin Cogné, Bertrand Isidor, Jens Meiler, Clémentine Ripoll, Stéphanie Bigou, Frédéric Laumonnier, Peter W Hildebrand, Evan E Eichler, Kirsty Mcwalter, Peter M Krawitz, Florence Roux-Dalvai, Ype Elgersma, Julien Marcoux, Marie-Pierre Bousquet, Arnaud Droit, Jeremie Poschmann, Andreas M Grabrucker, Francois V Bolduc, Stéphane Bézieau, Frédéric Ebstein, Elke Krüger

Faculty, Staff and Students Publications

Neurodevelopmental proteasomopathies are a group of disorders caused by variants in proteasome subunit genes, that disrupt protein homeostasis and brain development through poorly characterized mechanisms. Here, we report 26 distinct variants in PSMC5, encoding the AAA⁺ ATPase subunit PSMC5/RPT6, in individuals with syndromic neurodevelopmental conditions. Combining genetic, multi-omics and biochemical approaches across cellular models and Drosophila, we unveil the essential role of proteasomes in sustaining key cellular processes. Loss of PSMC5/RPT6 function impairs proteasome activity, leading to protein aggregation, disruption of mitochondrial homeostasis, and dysregulation of lipid metabolism and immune signaling. It also compromises synaptic balance, neuritogenesis, and neural progenitor …

Flash Effect Is Diminished By Daily Fractionation Of Electron Rt In Mouse Skin, Aleksandra Ilina, William S. Thomas, Xu Cao, Matthew S. Reed, Kendall Jarvis, Albert Van Der Kogel, Nathaniel Van Asselt, Wesley S. Culberson, Brian W. Pogue

Dartmouth Scholarship

Objective. While FLASH radiotherapy is known to reduce skin damage in vivo from ultra-high dose rate (UHDR) irradiation relative to conventional dose rates (CDR), it is not clear whether this sparing is preserved when delivered as fractionated. This study was designed to directly assess whether three daily fractions would maintain the sparing effects in murine leg models and preserve the murine skin sparing with single fraction treatments. Approach. C57Bl/6j female mice were irradiated with 9 MeV UHDR and CDR beams from a FLASH-capable Mobetron system, in a dose escalation study with doses ranging from single dose 22–30 Gy in a …

Maternal Obesogenic Diet Exposure Regulates The Offspring Gut Liver Axis And Fibroinflammatory Liver Disease, Naresh Naik Ramavath, Oğuz Özler, Holly Hinrichs, Francisco R Victorino, Vung Lian, Monica Young, Tarin M Bigley, Michael D Thompson

2020-Current year OA Pubs

Maternal obesogenic diet exposure (MODE) promotes fibroinflammatory liver disease in offspring via vertical transfer of an altered microbiome. The mechanism for how an altered offspring microbiome increases susceptibility to liver disease is not clear. A critical early life event termed the 'weaning reaction' is dependent on the early microbiome and when altered, results in worse pathologic inflammation. MODE attenuates the weaning reaction promoting worse liver disease in mice in an early microbiome-dependent manner. Using our MODE model and cross-fostering approaches we assessed the effect of MODE on neonatal gut-liver axis development. MODE shifts the bile acid (BA) profile, expression of …

El Pintor, Eryck León Tablada

Cuaderna Vía

No abstract provided.