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A Wasteside Story: Establishment And Evolution Of A Wastewater Surveillance Lab, Through The Pandemic And Beyond, Syed Shahrukh Hyder

Graduate Research Theses & Dissertations

Wastewater based epidemiology is a fast growing and practical solution to monitor the spread of disease within a population. As the COVID-19 pandemic grounded the world to a standstill, methods to monitor its spread and prepare appropriate responses became vital to begin the return to normalcy. Such was the case in universities around the world where the campus and surrounding community were vital in fulfilling the educational mission in the face of a generational health crisis. The population in a midsize higher educational institute such as Northern Illinois University (NIU) is a broad mix of on-campus and off campus students, …

The Energetics Of Seasonality In Wild Diademed Sifaka (Propithecus Diadema): A Technical And Biological Validation Of Fecal Triiodothyronine Radioimmunassay, Casey Farmer

Graduate Research Theses & Dissertations

The energetic state of an animal has vital consequences for its fitness, but it can be difficult to detect through behavioral observations. The development of techniques to determine an animals’ energetic state non-invasively would allow conservation planners to rapidly assess the energy balance and conservation potential of individuals, groups, and populations. Thyroid hormones like triiodothyronine (T3) regulate and reflect metabolic state in mammals. T3 is a non-invasive biomarker that can be detected in feces and could potentially be used for this purpose. I perform the first validation of a commercially available T3 radioimmunoassay designed for humans on a wild strepsirrhine …

Assessing The Relationship Between Neighborhood Inequality And Stem Employment For Underrepresented Minorities With Stem Degrees, Brian P. Hurley

Graduate Research Theses & Dissertations

The purpose of this study is to assess whether there is a relationship between neighborhood inequality in which underrepresented minorities (URMs) with STEM degrees reside and their employment in STEM fields. The current study deploys an Intersectional Theory (IT) research framework and Bourdieu’s Theory of Practice to identify the influence of neighborhood capital on successful matriculation through the STEM pipeline as well as variables that elucidate latent inequities in the STEM pipeline which lead to unequal outcomes for URMs. Generalized Linear Multilevel Models (GLMMs) are used to answer the research questions where the individual variables of gender, race, and age …

An Exploration Of The Sense Of Belonging For African American Women Licensed Clinicians In A Voluntary Professional Counseling Organization, Raushanah Jackson

Graduate Research Theses & Dissertations

Due to historical oppression in academe, the voices of African American women have been excluded, or unjustifiably limited, in academic research and from mainstream psychological thought. This study was one aim at understanding the experiences of belonging of women of African American ancestry who were clinically licensed professional members in a volunteer member counseling organization. Centering African American women’s voices was used through a lens of critical race theory and Black Feminist Thought to illuminate the structural, representational, and sociopolitical barriers that African American women face. An Interpretive Phenomenological Analysis approach was used to center the voices of five licensed …

Pathology And Diagnosis In Inflammatory-Related Diseases: Covid-19 And Age-Related Macular Degeneration, Zane Russell Lacasse

Graduate Research Theses & Dissertations

Chronic inflammation and dysfunctional inflammatory responses are believed to be contributing factors to the severity of many diseases. Since the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it has been recognized that levels of inflammatory markers and the severity of coronavirus disease-2019 (COVID-19) cases are closely related. The work within this dissertation begins with establishing an alternative platform for the diagnosis of COVID-19 and explores ways to enhance the diagnostic utility of this platform. An alternative route for COVID-19 diagnosis was explored using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS). The platform utilizes a non-invasive sampling technique …

Genomic Clustering And Comparative Analysis Of Sars-Cov-2 Variants And Subvariants In Year 2022, Fatemeh Nazaritiji

Graduate Research Theses & Dissertations

This study presents a bioinformatic analysis of SARS-CoV-2 genomes sampled in 2022, aimed at showing the genetic diversity and evolutionary patterns of the virus. A random selection of 1,000 genomes from a large collection of sequenced genomes was used to conduct an all-vs-all pairwise alignment, forming the basis for a genetic distance matrix utilizing thenumber of substitutions. This matrix served as a tool for clustering analysis, enabling the identification of distinct genomic variants and their relationships. Multidimensional Scaling (MDS) was employed to visualize the data in a two-dimensional space, revealing several distinct clusters that suggest the presence of diverse viral …

Mixed Mechanisms Of Multi-Site Phosphorylation, Suha Jayyousi Dajani

Graduate Research Theses & Dissertations

Multi-site phosphorylation is an important mechanism in cell biology that regulates protein function and activity. It also plays a critical role in a wide variety of cellular processes that control intra-cellular signaling. Studies on mono- and dual-site phosphorylation have been conducted theoretically and experimentally by researchers. However, there is little research on triple-site and multi-site mixed mechanism phosphorylation.

The aim of this research is to study and identify the number of positive steady states (multistationarity) in a mathematical model of a triple-site mixed mechanism, processive and distributive, phosphorylation network. This research is accomplished by means of ordinary differential equations, Jacobian …

On The Ground And From Above: A Multi-Factor Analysis Of The Direct And Indirect Drivers Of Tallgrass Prairie Small Mammal Communities, Erin Rowland-Schaefer

Graduate Research Theses & Dissertations

Tallgrass prairies are among the most globally threatened ecosystems, with only one percent of the historical extent remaining in Illinois. Prairie restoration is an essential strategy to preserve biodiversity and reestablish threatened species. Practitioners use management tools to shift the landscape context of prairie restoration sites, such as prescribed fire and land cover change. Managers can also impact the landscape connectivity and edge proximity of certain areas through the use of mowed driving lanes. The effects of these factors on small mammals, which are central in tallgrass prairie food webs and relevant to human health and restoration success, are not …

The Dna Double Stranded Break Damage Response Of U87 Gbm Cells Following Irradiation Treatment, Emma Louisa Planck

Graduate Research Theses & Dissertations

Glioblastoma Multiforme is one of the most aggressive forms of cancer and is becoming increasingly prevalent in the population, especially among young adults. An estimated 13,140 people in the United States will be diagnosed with GBM this year and an estimated 10,000 people in the United States will succumb to the disease. The 5-year survival for GBM patients is only 7.2% with a mean survival time of only 12-18 months. Even with subsequent chemotherapy and irradiation treatments GBM has a very high treatment resistance and has a recurrence rate of roughly 70%. Treatments such as radiation therapy induce lethal DNA …

Effort Of Reproducibility: Metrics, Methods, And Models To Navigate The Landscape Of Reproducible Research, Akhil Pandey Akella

Graduate Research Theses & Dissertations

Validating scientific claims is an elaborate process that involves researchers investing considerable time and effort to discover the validity of claims. It involves repeatability, reproducibility, and replicability, where each subsequent step requires increased effort and time. With the evolving nature of computational experiments and the exponential pace at which scientific publications are disseminated, researchers find it challenging to devote time, energy, and resources to assess claims made in papers of interest. Preemptive signals for reproducible effort can be helpful to researchers in filtering academic works of interest, thereby allowing them to adjust efforts based on need. Through this research, I …

How Students On A Healthcare Track Value Learning Communication Skills, Christina M. Cline

Graduate Research Theses & Dissertations

Communication is a critical component of effective healthcare. Students interested in going into healthcare fields require training to learn how to effectively communicate. These students have a need to learn communication skills. Understanding students’ value for learning these skills is necessary for increasing the motivation to learn. In this study, a mixed method approach was used to investigate the value that students on a healthcare track had for learning communication skills. Quantitative methods were used to develop a survey measuring student value and then evaluate students’ value when completing a communication skills activity in a gross human anatomy course. Qualitative …

Academic Grouping And School Sense Of Belonging: An Interpretive Phenomenological Analysis Of Middle School Student Voices, Kevin Cox

Graduate Research Theses & Dissertations

School sense of belonging is critical for students’ academic achievement and emotional well-being. The development of a sense of school belonging is influenced by direct and indirect factors within the school environment. Categorization into low and high achievement groups based on academics is a process largely outside the direct control of a student. This study employed an interpretive phenomenological analysis of interviews conducted with 17 seventh- and eighth-grade students about their belonging experience in a Midwestern public middle school. Experiences of students academically categorized as high, typical, or low achievers were compared and described in the context of three emergent …

Evaluation Of Conservation Strategies For Blanding’S Turtles (Emydoidea Blandingii), Callie Klatt Golba

Graduate Research Theses & Dissertations

Across the globe, biodiversity is threatened due to anthropogenic changes to the environment and large numbers of freshwater species, especially turtles, are facing population declines. Resources for conservation are limited, making empirically based conservation planning of utmost importance. Effective management strategies incorporate relevant biological information to outline specific and feasible actions that will mitigate the greatest threats to a species. Blanding’s Turtles (Emydoidea blandingii) are a long-lived, semi-aquatic, freshwater species that are classified as endangered in Illinois and internationally. As with other species of concern, Blanding’s Turtles are threatened by habitat loss, fragmentation, and degradation. They are also threatened by …

A Comparison Of Academic Achievement And Psychological Symptoms In Homeschooled And Traditionally Schooled Children: Accounting For Demographic And Family Characteristics, Lauren Elizabeth Hauck

Graduate Research Theses & Dissertations

The prevalence of homeschooling is increasing, especially in the aftermath of the COVID-19 pandemic. However, methodologically strong research examining how children's well-being may differ based on their schooling type (i.e., homeschooled vs. traditionally schooled) is rare. This study is a response to a call for advancing the methodological rigor and expanding our understanding of family contributions to differences between homeschooled and traditionally schooled children’s adjustment. Thus, the study aims to determine if differences between homeschooled and traditionally schooled (i.e., attending public school) children’s academic and emotional adjustment are present after accounting for both family demographic characteristics and parenting and home …

Ascertaining A Biochemical Approach To Chlorophyll D Biosynthesis In Acaryochloris Spp., Rashmi Shivni

Graduate Research Theses & Dissertations

Photosynthesis is one of the most abundant metabolic systems on Earth, and specifically, oxygenic photosynthesis primed the planet for complex, multicellular life. The hallmark of modern oxygenic photosynthesis is the photopigment chlorophyll a found in nearly all oxygenic phototrophs as their primary photopigment in their photosynthesis pathways. There are also many organisms that tap into a variety of chlorophylls to extend the energetic limits of photosynthesis, which are largely found as accessory pigments. One genus of cyanobacteria, Acaryochloris, has garnered attention for the ability to undergo photosynthesis by harnessing the far-red part of the visible light spectrum using chlorophyll d …

Expressive Writing Rooted In Self-Determination Theory: An Intervention For Racial Trauma, Diana A. Robinson

Graduate Research Theses & Dissertations

Understanding the impact of racial discrimination is not a new endeavor in the field of psychology. However, research utilizing a trauma-focused lens to evaluate the negative mental health outcomes in the aftermath of racial discrimination is only in its infancy. In addition to depression, anxiety, and substance use, recent research has linked racial discrimination to negative mental health outcomes that mirror that of posttraumatic stress disorder (PTSD). Although many researchers have identified the negative effects of racial discrimination, few studies have examined intervention approaches designed to reduce the negative impact of racial discrimination. Self-Determination Theory (SDT) is a comprehensive theory …

Reinterpreting Fort Ancient Plant Relationships Through Native Science And Traditional Ecological Knowledge, Bailey Rae Raab

Graduate Research Theses & Dissertations

Paleoethnobotanical analyses over the past several decades have shed light on subsistence practices, agricultural strategies, and environmental interactions of members of the Fort Ancient culture, an Indigenous society that thrived in the Ohio Valley from 1000 to 1650 CE. Largely absent from these conversations, however, are discussions of non-food uses of plants or considerations of Traditional Ecological Knowledge (TEK). By combining multiple different sources of knowledge, a more holistic view of people-plant relationships within the Fort Ancient culture may be established, one that allows us to better understand these relationships in the past and present. In this thesis, I synthesize …

Iodp U1537: Scotia Sea Climatic And Frontal Variation Reflected In Diatom Productivity And Assemblages Since The Lgm, Garrett Lewis Strittmatter

Graduate Research Theses & Dissertations

Located off the coast of the West Antarctic Ice Sheet, the Scotia Sea is a site of great significance in palaeoclimatological studies due to the nature of its sedimentary record. This site of interest is at the tip of the Antarctic Peninsula on the Eastern side, in the direct flow of the Antarctic Circumpolar Current thereby concentrating icebergs from around the continent. Additionally, these sediments are rich in diatoms. Finally, this site, within the analyzed section, has an average sedimentation rate of ~48 cm/Kyr.

By collecting sediment cores rich in diatoms, among material used to generate other paleoclimate proxies, we …

An Environmental Isolate Of Pseudomonas, 20ei1, Reduces Aspergillus Flavus Growth In An Iron-Dependent Manner Affecting The Expression Of Numerous Genes, Elizabeth Marion Wyman

Graduate Research Theses & Dissertations

Aspergillus flavus is an opportunistic pathogenic fungus that infects oilseed crops worldwide. When colonizing the plants, it produces mycotoxins, including carcinogenic compounds such as aflatoxins. Mycotoxin contamination results in an important economic and health impact. The design of new strategies to control A. flavus colonization and mycotoxin contamination is paramount. In this study, we identified a promising new isolate of Pseudomonas spp., 20EI1, and observed that it is able to reduce the growth of A. flavus AF70. Furthermore, we determined that this growth inhibition is iron-dependent. To further elucidate the nature of this bacterial-fungus interaction, we performed metabolomics and transcriptomics …

Trajectory Of Infant Emotion Regulation Strategies And Negative Affect And Associations With Toddler Externalizing Problems, Sabrina D. Ung

Graduate Research Theses & Dissertations

Previous research recognizes the critical role played by child emotion regulation (ER), the regulatory component of temperament, and negative emotionality (i.e., the tendency to experience negative emotions such as fear, sadness, anger, and frustration) in emotional and behavioral functioning throughout the lifespan. Poor ER and high negative affect in early childhood are linked to impulsive and aggressive behaviors, internalizing behaviors, and lower cognitive functioning. However, few studies have investigated how specific ER strategies and negative affect change across infancy by employing latent growth modeling. Additionally, existing work has neglected to examine the growth trajectory of escape behaviors across infancy as …

Dna I-Motifs: Biophysical Characterization And Structure-Property Correlation As Foundation For Functional Material Design, Alexander Minasyan

Graduate Research Theses & Dissertations

Over the past decades, nucleic acids have emerged as crucial frameworks with significance in biological systems and functional material engineering. Cytosine-rich deoxyribonucleotide sequences can form quadruplex structures a.k.a. i-motifs. Recently, i-motifs have attracted scientific interest in biological and material design areas.

In this work, we systematically investigate an effect of structured and unstructured modifications such as hairpins, stems, loops and overhangs on the kinetic and thermodynamic properties of DNA i-motifs. We demonstrate that the modifications can influence i-motif’s stability and folding/unfolding rates: thus, double stranded stems and hairpins are the best at stabilizing i-motifs and increasing kinetic rates of folding. …

Self-Compassion Intervention For Shame In Trauma-Exposed Gender And Sexual Minorities, Kyla A. Leonard

Graduate Research Theses & Dissertations

Individuals who identify as gender or sexual minorities (e.g., lesbian, gay, bisexual, pansexual, asexual, queer, transgender, nonbinary, intersex, gender nonconforming, etc.) have a high prevalence of interpersonal trauma. These individuals also experience a higher likelihood of experiencing posttraumatic stress disorder (PTSD) symptoms after exposure to potentially traumatic events. It has also been shown that those who identify as gender or sexual minorities experience high rates of shame due to minority stress and psychological factors such as internalized heterosexism. Experiences of shame are a central component in the development and maintenance of PTSD symptoms, particularly within this population. Self-compassion is an …

Producing Biocomposites From Hemp Residues, Mohammad Faghani

Graduate Research Theses & Dissertations

The significant environmental challenges posed by traditional fossil fuel-derived plastics necessitate the exploration of sustainable alternatives. The global production of plastic has dramatically increased from 2 million tons in 1950 to 368 million tons in 2019, with projections exceeding 600 million tons by 2025. By 2040, the greenhouse gas emissions from the lifecycle of these plastics are expected to consume 19% of the global carbon budget. The United States alone produces nearly twice as much plastic waste as China and more than the entire European Union combined, highlighting the urgency of finding eco-friendly solutions.

Biocomposites, combining natural fibers with either …

Re-Feminizing The Divine: Understanding The Cultural Constructs Of Gender And Sexuality In A Church-Based Christian Community, Cameron K. Thomas

Graduate Research Theses & Dissertations

Historically, Christianity has sought to maintain rigid separation between men and women. Christianity often views women as secondary to men, particularly in relation to authority under Jesus Christ. These ideals have led to the restrictions of rights and roles of people who choose not to adhere to strictly defined or enforced gender roles. For this ethnography, I examined one church community; namely Barbwire Baptist Church in northern Illinois and how its members used scripture and doctrine beyond simply worship to reinforce norms and expectations of self and others regarding gender and sexuality. I draw on scholarship in gender studies, and …

Motivation, Engagement, And The Student-Teacher Relationship As Predictors Of Academic Outcomes, Courtney Weaver

Graduate Research Theses & Dissertations

Motivation and engagement have long been studied in educational literature as factors that contribute to student academic achievement. The student-teacher relationship has also gained attention as a factor that exerts influence on students’ performance in the classroom. Models that consider context, or the ways in which these student- and teacher-level factors work together, are lacking in the literature. The current study employed an ecological systems framework with concepts from two related theories, attachment and self-determination, to investigate the roles of motivation, engagement, closeness and conflict in the student-teacher relationship as influences on student academic outcomes. Data for the current study …

Bidirectional In-Situ Analysis And Visualization Of Fluid Structure Interactions With Applications In Blood Flow, Nazariy Tishchenko

Graduate Research Theses & Dissertations

Interactive blood flow simulation has applications in the understanding of complex fluids,training medical professionals, diagnostics, and surgical procedure planning. Bidirectional in situ visualization and analysis are much needed to streamline the analysis of large-scale patient-specific blood flow simulations. The in situ component ensures the live visualization of the simulation results while the simulation is running, and the bidirectional component allows for real-time control and configuration of the simulation parameters (i.e., setting and retrieving the simulation parameters and data based on need). In this project, a highly accurate multiphysics simulation was instrumented to work with an existing bidirectional in situ framework. …

Social Gender Norms And Depression In College Students, Derek Deeney

Masters Theses

The primary purpose of this quantitative study was to determine if there was a relationship between the conformity to social gender norms (masculine and feminine) and depression among male and female college students at a mid-sized, four-year public institution in the Midwest. A secondary purpose was to investigate if there was a difference in depression between males and females, and if a there was a difference in conformity to social gender norms (masculine and feminine) between males and females. Understanding the trends in social norm conformity and depression can lead to changes in how faculty and staff in higher education …

Taking A Look At The Chicago Renaissance: A Cultural And Influential Movement, Shannon Watkins

Masters Theses

A resource guide for educators to allow students to explore literature from the Chicago Renaissance; while bridging the gap between Social Studies and English/Language Arts. This guide has lesson plans, curricular resources, and references for Intermediate Elementary teachers and students. Lesson plans are aligned with Bloom’s Taxonomy, Illinois State & National Standards. This guide implements Common Core State Standards with interdisciplinary subject areas while developing and emerging critical thinkers in students.

Are Elaeagnus Umbellata Invasions Limited By Microbial Interactions?, Cody Anderson Leverette

Masters Theses

Mutualistic interactions with soil microbes are a key component of plant invasions. Mutualists such as ectomycorrhizal fungi and Rhizobia bacteria often limit invasive plant establishment and performance, but the role of nitrogen-fixing Frankia bacteria in invasions remains largely unexplored. In this study, I quantified the net effects of positive and negative plant-soil feedbacks, including Frankia nodulation, on invasive Elaeagnus umbellata (autumn olive; hereafter AO) seedling performance and traits at varying distances from focal shrubs using live soil and sterilized soil. The expectation was that seedling performance would decrease with further distance from shrubs as Frankia abundance decreased. Contrary to expectations, …

Investigating Sleep Education Criteria In Illinois Public Schools And Its Alignment With Evidence-Based Practices, Bayleigh T. Pasley

Masters Theses

The purpose of the current study was to examine high school health curricula regarding sleep prevention and intervention practices and whether instructional content aligned with best practice for this age group. Specifically, the Primary Investigator and her thesis chair developed the Sleep Education and Preventative Practices Checklist/Questionnaire (SEPPC and SEPPQ) as tools to evaluate the sleep content covered in different public high schools across Illinois. The SEPPC and SEPPQ were identical in that they contained 17 different items which looked at different sleep topics reviewed by current literature. The SEPPC/Q contained two main topics: adolescent sleep knowledge (11 topics/items) and …