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2024

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Icvd Polymer Thin Film Bio-Interface-Performance For Fibroblasts, Cancer-Cells, And Viruses Connected To Their Functional Groups And In Silico Studies, Asmaa Taha Jan 2024

Icvd Polymer Thin Film Bio-Interface-Performance For Fibroblasts, Cancer-Cells, And Viruses Connected To Their Functional Groups And In Silico Studies, Asmaa Taha

Nanotechnology Research Centre

Thin polymer coatings are used to improve the interface between biological species and functional materials. Their interaction is significantly influenced by the functional groups and roughness of the polymer film and prediction of the interaction is thus of great interest. However, for conventional polymer films, this cannot be examined independently because of the interplay of defects, residual solvent molecules, roughness, and functional groups. Solvent-free polymer films prepared by initiated chemical vapor deposition (iCVD) exhibit conformal, defect-free characteristics and enable precise tailoring of the functional groups. This facilitates to isolate the contribution of functional groups on the bio-interface performance. Consequently, in …

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Front Matter, Natural Resources Journal Jan 2024

Front Matter, Natural Resources Journal

Natural Resources Journal

No abstract provided.

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Introduction, Miguel A. Quintana, Logan Stokes Jan 2024

Introduction, Miguel A. Quintana, Logan Stokes

Natural Resources Journal

No abstract provided.

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A Half-Century Of Pacific Salmon Saving Efforts: A Primer On Law, Policy, And Biology, Michael C. Blumm, Daniel J. Rohlf, Adam Eno Jan 2024

A Half-Century Of Pacific Salmon Saving Efforts: A Primer On Law, Policy, And Biology, Michael C. Blumm, Daniel J. Rohlf, Adam Eno

Natural Resources Journal

Pacific salmon, the signature species of the Pacific Northwest, have declined across their range for well over a century, due to a myriad of human-caused effects on their habitat and the fish themselves. Restoration efforts—some successful, some halting—began in earnest in the late 20th century, with considerable attention focused on the Columbia Basin, where historically salmon runs were crippled by a large interconnected hydroelectric system of federal and non-federal dams. In the 1980 Northwest Power Act, Congress created an interstate agency, the Northwest Power and Conservation Council, with access to a substantial amount of ratepayer dollars; the agency has chosen …

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Symposium On New Mexico's Just Transition, Melanie Coffing, Logan Stokes Jan 2024

Symposium On New Mexico's Just Transition, Melanie Coffing, Logan Stokes

Natural Resources Journal

A Just Transition aims to develop capacity for a low-carbon, climate-resilient economy while maximizing benefits and minimizing hardships for working communities. The ideation and implementation of such a sweeping, intersectional policy framework requires thoughtful conversation, collaborative action, and years of dedication from community members and policy makers alike. On November 3, 2023, the Natural Resources Journal, through the University of New Mexico School of Law, and the New Mexico Speaker of the House, Javier Martínez, hosted the Symposium on New Mexico’s Just Transition. Students from the law school and the Natural Resources Journal had the opportunity to collaborate with community …

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Epigenetic Regulation Of Cancer Testis Antigens In Hepatocellular Carcinoma, Rinrada Klankajorn Jan 2024

Epigenetic Regulation Of Cancer Testis Antigens In Hepatocellular Carcinoma, Rinrada Klankajorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hepatocellular carcinoma (HCC) represents one of the most common causes of cancer-related death worldwide, with few available treatment options. Cancer-testis antigens (CTAs), notably from the MAGE gene family, have opened new possibilities for improving immunotherapy in HCC due to their tumor-specific expression and high immunogenicity. However, the mechanisms regulating MAGE gene expression are largely unknown. This study explored mechanisms controlling the expression of MAGE-A3 and MAGE-C2. We utilized HCC organoid cultures derived from patient HCC tissues known to represent cancer epigenetic status more accurately than cell lines to study the MAGE gene family expression patterns and promoter methylation patterns. Our …

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Acute Toxicity Study Of Canine Mesenchymal Stem Cell (Msc)-Derived Exosome Preparation For Drug Registration, Anatcha Thongsit Jan 2024

Acute Toxicity Study Of Canine Mesenchymal Stem Cell (Msc)-Derived Exosome Preparation For Drug Registration, Anatcha Thongsit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Exosomes, spherical vesicles secreted by cells containing diverse biomolecules, are gaining attention in veterinary medicine. Mesenchymal stem cell (MSC)-derived exosomes show promise for treating animal diseases. However, their safety profile, particularly the acute toxicity of canine MSC-derived exosomes, has yet to be adequately explored. This study evaluates the acute toxicity of canine MSC-derived exosomes on rat systemic health, adhering to ISO 10993-11 guidelines for systemic toxicity testing to support veterinary drug registration and development. The study tested acute toxicity in five female Sprague Dawley rats by administering exosomes at a concentration of 1×109 particles/mL via intravenous injection into the tail …

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Expression Optimization And Characterization Of Plant-Produced Human Interleukin-15, Chalatorn Charnsatabut Jan 2024

Expression Optimization And Characterization Of Plant-Produced Human Interleukin-15, Chalatorn Charnsatabut

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human interleukin-15 (hIL-15) is a cytokine that plays a crucial role in immune responses like T cell proliferation, activation, and prolongation of natural killer (NK) cells. This cytokine also impacts the cell proliferation of human adult low calcium temperature (HaCaT) keratinocytes which can lead to the wound-healing effect. This study aimed to optimize the expression and characterize the recombinant hIL-15 in Nicotiana benthamiana with the Agrobacterium-mediated transient expression system. The hIL-15 gene was fused with the Fc domain from the IgG1 antibody for purification. The expression cassette was optimized for sub-cellular targeting and agroinfiltration. The transiently expressed hIL-15-Fc was purified …

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Molecular Mechanism Of Lusianthridin On Platelet Aggregation Inhibition And Endothelial Cell Protection, Fozia - Jan 2024

Molecular Mechanism Of Lusianthridin On Platelet Aggregation Inhibition And Endothelial Cell Protection, Fozia -

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lusianthridin (LST), a bioactive compound isolated from Dendrobium venustum, exhibits notable vasoprotective properties. This study aimed to elucidate the underlying molecular mechanisms of LST by evaluating its effects on platelet function and endothelial cell activation in vitro. In human platelets, LST significantly inhibited collagen-induced aggregation, an effect associated with elevated phosphorylation of vasodilator-stimulated phosphoprotein (VASP) and suppression of Akt phosphorylation. In EA.hy926 endothelial cells, LST attenuated tumor necrosis factor-alpha (TNF-α)-induced endothelial dysfunction by reducing nitric oxide (NO) production and suppressing the expression of pro-inflammatory cytokines, including interleukin-6 (IL-6) and interleukin-1β (IL-1β). Mechanistic investigations revealed that LST inhibited key inflammatory signaling …

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Predicting The Eq-5d-5l Utility Scores From The Impact Of Vision Impairment (Ivi) Questionnaire In Thai Patients, Emilia Audrey Tunmas Pasenggong Jan 2024

Predicting The Eq-5d-5l Utility Scores From The Impact Of Vision Impairment (Ivi) Questionnaire In Thai Patients, Emilia Audrey Tunmas Pasenggong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives : (1) This study aims to predict the EQ-5D-5L utility scores from the impact of vision impairment (IVI) questionnaire in Thai patients using mapping techniques. (2) To investigate the association between sociodemographic and clinical variables with the EQ-5D-5L index scores. Methods : This is a secondary data analysis. A total of 499 patients with multiple levels of visual impairment were recruited from King Chulalongkorn Memorial Hospital Thailand between February and July 2022. Ordinary least square (OLS), Tobit, Censored least absolute deviation (CLAD), and Adjusted limited dependent variable mixture model (ALDVMM) regression models were used to map the IVI questionnaire …

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Development Of Granule Product Containing Curcuminoid Extract And Hyaluronic Acid Derivative For Oral Administration, Natkhanang Boonpetcharat Jan 2024

Development Of Granule Product Containing Curcuminoid Extract And Hyaluronic Acid Derivative For Oral Administration, Natkhanang Boonpetcharat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Curcumin is widely recognized for its various pharmacological properties, including antioxidant, anti-inflammatory, and anti-tumor activities. Nevertheless, the development of curcumin as a therapeutic agent is impeded by its limited oral bioavailability, which stems from its chemical instability, poor aqueous solubility, and rapid degradation. This study aimed to develop granule formulations incorporating hyaluronic acid graft poly(N-isopropylacrylamide) or HA-g-pNIPAM to enhance dissolution and protect curcumin from degradation. Three formulations were developed: F10 (HA-g-pNIPAM physically mixed with curcumin), F10 Encap (curcumin encapsulated within HA-g-pNIPAM), and F11 (curcumin granules without HA-g-pNIPAM). The stability results showed that F10 Encap effectively maintained curcumin content throughout the …

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The Effect Of Stilbene Derivatives On Modulation Of P-Gp Efflux Transporter, Shahnila Mukhtar Jan 2024

The Effect Of Stilbene Derivatives On Modulation Of P-Gp Efflux Transporter, Shahnila Mukhtar

Chulalongkorn University Theses and Dissertations (Chula ETD)

Drug efflux by transporters of the ATP-binding cassette (ABC) family such as P-glycoprotein is the earliest and most well-studied multidrug resistant (MDR) mechanism. Overexpression of ATP-binding cassette transporters can decrease the effectiveness of chemotherapy by pumping anti-cancer drugs out of cancer cells. This study was to predict the best-fit of certain stilbenoids isolated from Thai orchids as a specific P-gp moderator, using the molecular docking approach to compare and specify the binding interaction between each stilbenoids and the P-gp binding domains. Furthermore, the in vitro substrate uptake assays of eight stilbenoids with phenanthrene core structure were conducted for assessing their …

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The Effect Of Artogomezianone On Apoptosis Induction In Non-Small-Cell Lung Cancer, Worawich Somboonvoravong Jan 2024

The Effect Of Artogomezianone On Apoptosis Induction In Non-Small-Cell Lung Cancer, Worawich Somboonvoravong

Chulalongkorn University Theses and Dissertations (Chula ETD)

NSCLC severity is driven by dysregulation of the EGFR/PI3K/Akt pathway, promoting tumor growth and drug resistance. Hyperactive EGFR/PI3K/Akt signaling results in aggressive tumors and poor outcomes, further worsened by resistance to EGFR inhibitors. Targeting this pathway could inhibit tumor growth and resistance. artogomezianone, a compound derived from Artocarpus gomezianus, has shown considerable anti-cancer efficacy, mainly by inducing apoptosis and decreasing the proliferation of several cancers. This study investigated artogomezianone’s effects on the EGFR/PI3K/Akt pathway. Cytotoxic and apoptotic effects were assessed using MTT assay and Hoechst 33342/PI double staining, with confirmation by Western blot analysis. Protein levels and binding affinity were …

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Effect Of An Mrna Vaccine On Liver-Associated Macrophages And The Immunogenicity Of Ag85b-Mrna Vaccine Against Tuberculosis, Chitsuda Pongma Jan 2024

Effect Of An Mrna Vaccine On Liver-Associated Macrophages And The Immunogenicity Of Ag85b-Mrna Vaccine Against Tuberculosis, Chitsuda Pongma

Chulalongkorn University Theses and Dissertations (Chula ETD)

A new vaccine technology based on the delivery of mRNA has gained widespread acceptance due to the COVID-19 pandemic. The delivery of mRNA formulated with lipid nanoparticles (LNPs) elicits a robust immune response, activating both humoral and cell-mediated response. Depending on the LNP formula, mRNA encoding proteins can be detected in the liver upon intramuscular (IM) administration in mice. Thus, an mRNA vaccine present novel platform for new vaccines for infection diseases, including tuberculosis (TB). This study proposed two major goals to investigate the impact of an mRNA vaccine on innate immune cells in the liver and the use of …

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Immune Signaling And Pathogenesis Of Enterocytozoon Hepatopenaei Infection In Pacific White Shrimp Litopenaeus Vannamei, Nutthapon Sangklai Jan 2024

Immune Signaling And Pathogenesis Of Enterocytozoon Hepatopenaei Infection In Pacific White Shrimp Litopenaeus Vannamei, Nutthapon Sangklai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Enterocytozoon hepatopenaei (EHP) is an intracellular microsporidian parasite causing hepatopancreatic microsporidiosis (HPM), a disease that impairs the digestion and nutrients absorption of shrimp and leads to growth retardation and size variation. Our studies aim to comprehensively investigate the host’s immune responses to the EHP infection and the pathogenesis of the parasite. It was found that genes in Toll and JAK/STAT pathways, as well as antimicrobial peptides, were highly upregulated upon the EHP infection. Importantly, suppression of the JAK/STAT pathways resulted in a significant reduction of the c-type lysozyme (LvLyz-c) expression and the elevation of EHP copy numbers. These indicate a …

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Development Of Biosensors For Detection Of Human Papillomavirus Type 16 Dna, Panon Tungkunaruk Jan 2024

Development Of Biosensors For Detection Of Human Papillomavirus Type 16 Dna, Panon Tungkunaruk

Chulalongkorn University Theses and Dissertations (Chula ETD)

This dissertation aims to develop two unconventional methods for the detection of a high-risk, cancer-causing human papillomavirus type 16 (HPV16) on miniaturized platforms for point-of-care testing (POCT). The first part focuses on a direct detection of HPV16 double-stranded DNA (dsDNA) using the non-self-pairing pyrrolidinyl peptide nucleic acid (acpcPNA) pair on a paper-based substrate coupled with fluorescence and electrochemical dual detections using a DNA-binding dye in a single device. The acpcPNA pair can perform double duplex invasion into dsDNA by hybridizing with each strand of DNA in the same region without the requirement of denaturing dsDNA to single-stranded DNA (ssDNA) before …

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Development Of Electrochemical Sensor Based On Molecularly Imprinted Polymer For Determination Of Roxarsone, Pitranan Kunthanasap Jan 2024

Development Of Electrochemical Sensor Based On Molecularly Imprinted Polymer For Determination Of Roxarsone, Pitranan Kunthanasap

Chulalongkorn University Theses and Dissertations (Chula ETD)

Roxarsone (ROX), a growth enhancer in poultry and swine, has the potential to improve weight gain and prevent coccidiosis. Excessive use of ROX may lead to its accumulation in animal tissues, potentially contaminating food products and posing health risks to humans through consumption. This research aims to develop an electrochemical sensor based on a magnetic screen-printed graphene electrode (mSPGE) modified with a highly selective molecularly imprinted polymer (MIP) for the determination of ROX with enhanced sensitivity and selectivity. Typically, an applied magnetic field at the mSPGE could accelerate mass transport at the electrode surface through the magnetohydrodynamic (MHD) effect, leading …

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Andrews University Press 2024 Booklist, Andrews University Press Jan 2024

Andrews University Press 2024 Booklist, Andrews University Press

Booklists

No abstract provided.

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(2024) Different Maps, Same Territory: Dialogical Self Theory And Its Predecessors, David Kennedy Jan 2024

(2024) Different Maps, Same Territory: Dialogical Self Theory And Its Predecessors, David Kennedy

Collected Papers of David Kennedy

Dialogical Self Theory has not emerged in a historical vacuum. Its fore-runners, whether the Eastern Atman, the tripartite Platonic self with itsclear hierarchical relations, the Cartesian “ghost in the machine,” theFreudian ego-id hydraulic model or the Jungian topography of ego andunconscious, represent the theoretical grounding from which the dia-logical self has emerged and self-articulated as much as have WilliamJames, George Herbert Mead and Mikel Bakhtin. Most of them, includ-ing DST, center on developmental models, and see the self as intrinsi-cally oriented toward a more integrated sense of itself that Jung called“wholeness,” although their internal politics differ. Plato is an authoritar-ian, …

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“With Intent To Destroy, In Whole Or In Part”: Genocide, Ethnic Cleansing, And A Lost History, Alexander K.A. Greenawalt Jan 2024

“With Intent To Destroy, In Whole Or In Part”: Genocide, Ethnic Cleansing, And A Lost History, Alexander K.A. Greenawalt

Elisabeth Haub School of Law Faculty Publications

Drawing upon original research into the travaux préparatoires of the 1948 Genocide Convention, this Article advances several claims that complicate the standard account according to which genocide must entail a purpose to physically destroy at least a substantial part of a protected group. The core of the Article closely explores the words “intent,” “destroy,” and “in part,” showing how international authorities have settled on a received and largely uninterrogated wisdom regarding the meaning of these terms, one which is supported neither by the drafting history of the Genocide Convention, nor even by the actual results of the judicial decisions that …

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Conceptualizing Language Ideologies About Education: Exploring Perceptions Of Multilingualism Among Marathi Speakers From India, Priya M. Dabak, Jessica Sujata Chandras Jan 2024

Conceptualizing Language Ideologies About Education: Exploring Perceptions Of Multilingualism Among Marathi Speakers From India, Priya M. Dabak, Jessica Sujata Chandras

UNF Faculty Research and Scholarship

Perceptions of multilingualism have an impact on the educational choices of urban Indians and larger policy decisions around language in education in the Indian state of Maharashtra. By surveying 150 speakers of Marathi, the official language of the state and mother tongue, or first language of survey respondents, perceptions of multilingualism reveal conceptual assumptions and biases that can be uncovered through cognitive-linguistic analysis. Unprompted, survey respondents drew connections between multilingualism and education, underscoring language ideologies, or beliefs about languages which motivate urban Maharashtrians' educational decisions. Education is the stage upon which multilingual speakers in India express their aspirations and anxieties …

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Calcium Permeability Of Rat Trpv1 In A Close State When Expressed In Xenopus Laevis Oocytes, Zinnia Adhikary Jan 2024

Calcium Permeability Of Rat Trpv1 In A Close State When Expressed In Xenopus Laevis Oocytes, Zinnia Adhikary

Theses and Dissertations

Transient Receptor Potential V1 (TRPV1) is a nonselective cation channel, which is expressed in nociceptors/ pain detecting receptors. Activated by a wide variety of exogenous and endogenous physical and chemical stimuli such as heat, TRPV1 is involved in temperature detection, body temperature regulation, and pain perception. It is generally accepted that TRPV1's conductance to cations, including Ca2+, depends on its conformational change from a closed to an open state following gating. In the current study, I observed that the plasma membrane of Xenopus laevis oocytes, upon exogenous expression of rat TRPV1, exhibited weak permeability to Ca2+, as evidenced by the …

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Searching For Novel Mutations That Regulates The Mouse Polycystin-2 (Trpp2) Ion Channel, Akhilraj Rajan Pillai Jan 2024

Searching For Novel Mutations That Regulates The Mouse Polycystin-2 (Trpp2) Ion Channel, Akhilraj Rajan Pillai

Theses and Dissertations

Polycystin-2 (PC2) is an ion channel belonging to the polycystin subfamily of the transient receptor potential (TRP) cation channel superfamily. Mutations in PC2 are associated with autosomal dominant polycystic kidney disease (ADPKD), one of the most common genetic diseases in humans. Despite extensive studies over the past decades, the role of PC2 in normal and ADPKD kidneys remains largely unknown, and the activation and regulation of this ion channel are elusive. To gain a better understanding of the structure and function of the mouse PC2 channel, this study focused on generating five pore mutations in a gain-of-function (GOF) mutant of …

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Illustrating The Fantastic: Cross-Writing Illustrations In British Children’S Literature Of The Golden Age, Stephanie Montalti Jan 2024

Illustrating The Fantastic: Cross-Writing Illustrations In British Children’S Literature Of The Golden Age, Stephanie Montalti

Theses and Dissertations

This dissertation argues that British children’s literature of the Golden Age addresses children and adults through their illustrations. It considers why the nineteenth century experienced a proliferation in fantasy and illustration, as well as why Golden Age children’s texts appeal to mixed-age audiences. Through an application of the term “cross-writing,” I argue that the Golden Age of children’s literature did not think of audiences in a binary manner, as some children’s literature scholars tend to assert. Instead, they used the dual mode of textual and visual narration to complicate the boundaries between childhood and adulthood. I also offer an original …

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Exploring The Dual Role Of Vegf165 In Bleomycin Induced Lung Injury And Recovery In A Aav Mice Model, Igor Ban Jan 2024

Exploring The Dual Role Of Vegf165 In Bleomycin Induced Lung Injury And Recovery In A Aav Mice Model, Igor Ban

Theses and Dissertations

Pulmonary fibrosis (PF) poses a significant challenge in respiratory medicine due to its complex etiology and progression characterized by excessive lung scarring, leading to compromised pulmonary function. The multifaceted roles of Vascular Endothelial Growth Factor A (VEGF-A) and specifically its isoform VEGF-A165 are critical in this context, given their involvement in angiogenesis, lymphangiogenesis, and tissue repair. The dual nature of VEGF-A165, promoting angiogenesis and potentially exacerbating or mitigating fibrosis, underscores its significance in both the progression and recovery phases of PF. Our work, explores the nuanced functions of VEGF-A165 using AAV1 as a gene delivery vector to overexpress this growth …

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A Quality-By-Design Approach For The Development Of Inhalable Dry Powders For Tuberculosis (Tb) Treatment, Suyash M. Patil Jan 2024

A Quality-By-Design Approach For The Development Of Inhalable Dry Powders For Tuberculosis (Tb) Treatment, Suyash M. Patil

Theses and Dissertations

Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), remains a pressing concern globally. The emergence of drug-resistant strains and the need for prolonged treatment regimens continue to complicate disease management. Innovative therapeutic strategies are urgently required to enhance treatment efficacy, improve patient adherence, and minimize adverse effects. Toward this, strategies involving use of drug combinations, reducing dosing frequencies, and targeting TB-infected cells are gaining attention. In my dissertation, we explored the combination therapy involving two FDA-approved anti-TB drugs, Bedaquiline (BDQ) and Pretomanid (PTD). The BDQ-PTD combination reduced minimum inhibitory concentration (MIC), compared to individual drugs and demonstrated additive/synergistic effects at 1:4 …

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The Additionality Double Standard, James Salzman Jan 2024

The Additionality Double Standard, James Salzman

Public Law and Legal Theory Working Papers

Carbon offsets are widely criticized for lacking additionality. The public debate, how- ever, has failed to recognize that the problem of additionality goes far beyond offsets. It arises any time the government subsidizes an activity. Programs with longer histories than offsets and far more money at stake offer valuable lessons for managing lack of additionality in offsets. The article sets out the current efforts to prove offset additionality, explains why major government programs present comparable additionality challenges, and show why other programs’ problems of additionality are perceived as significantly different than those for offsets. We examine the toolkit used to …

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Long Term Trends In Mortality Rates Of A Smallmouth Bass Population, Caleb D. Anderson Jan 2024

Long Term Trends In Mortality Rates Of A Smallmouth Bass Population, Caleb D. Anderson

Journal of Earth and Life Science

Smallmouth bass Micropterus dolomieu are known as a popular sport fish for anglers. When a known smallmouth bass fishery appears to have a dwindling population, it raises questions about the population health. A common indicator of the health of the population is the mortality rate. By comparing mortality, conclusions can be made on whether smallmouth bass are dying at a different rate in the system over time. Therefore, the objective of this study was to see if there had been a significant change in smallmouth bass mortality over a span of thirty years in Round Lake (DOW:010204), Aitkin County, Minnesota. …

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Relationship Between Northern Pike And Walleye Cpue In Minnesota Lakes, Simon Oslund Jan 2024

Relationship Between Northern Pike And Walleye Cpue In Minnesota Lakes, Simon Oslund

Journal of Earth and Life Science

Northern pike Esox lucius and walleye Sander vitreus are both top predators in freshwater systems. Due to their predatorial nature, high populations of one species can prohibit populations of the other species from thriving, specifically northern pike foraging on walleye. This information is tracked by the Minnesota Department of Natural Resources through catch per unit effort (CPUE). CPUE data was gathered for all lakes in Beltrami County, Minnesota, and a regression analysis was done to test for a relationship between pike and walleye. It was found that the relationship between northern pike and walleye CPUE was significant (P = 0.02). …

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The Effects Of Length, Weight, Age, And Gender On Mercury Concentrations In Burbot In North-Central Minnesota Lakes, Liam Hanley Jan 2024

The Effects Of Length, Weight, Age, And Gender On Mercury Concentrations In Burbot In North-Central Minnesota Lakes, Liam Hanley

Journal of Earth and Life Science

Burbot Lota lota in north-central Minnesota lakes have the potential to reach large sizes and consume large volumes of prey. This caveat may make burbot susceptible to higher rates of biocontamination, bioaccumulation and biomagnification. The objective of this study was to determine how changes in age, length, gender, weight, and lake affect total mercury concentrations in burbot. In this experiment 28 burbot were angled from three lakes: Cass (n = 17), Winnibigoshish (n = 4), and Bad Medicine (n = 7). Then tissue samples were taken from each fish and were lyophilized and homogenized. Homogenized tissue samples were analyzed by …

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