Digital Commons Network^™

The Rna Receptor Rig-I Binding Synthetic Oligodeoxynucleotide Promotes Pneumonia Survival, Yongxing Wang, Vikram V Kulkarni, Jezreel Pantaleóngarcía, Michael K Longmire, Mathilde Lethier, Stephen Cusack, Scott E Evans

Faculty, Staff and Student Publications

Pneumonia is a worldwide threat to public health, demanding novel preventative and therapeutic strategies. The lung epithelium is a critical environmental interface that functions as a physical barrier to pathogen invasion while also actively sensing and responding to pathogens. We have reported that stimulating lung epithelial cells with a combination therapeutic consisting of a diacylated lipopeptide and a synthetic CpG oligodeoxynucleotide (ODN) induces synergistic pneumonia protection against a wide range of pathogens. We report here that mice deficient in TLR9, the previously described receptor for ODN, still displayed partial ODN-induced protection. This prompted us to seek an alternate ODN receptor, …

Comment Letter For The Butte Priority Soils Operable Unit (Bpsou) Draft Reclaimed Areas Weed Management Plan (Dated April 11, 2024), Molly Roby

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

The Diamond, November 8, 2024, Dordt University

Dordt Diamond

• Front Page: Dordt Students to Attend UN Climate Change Conference; Election Night Watch Party: Dordt Students Witness History in the Making; Retired Missionary and Wife of Martyr, Phyliss Masters Gives Testimony at Dordt
• News: Cell Phone Bans Spreading Throughout Northwest Iowa Schools; Dodgers Beat Yankees 4-1 to Win World Series; Students Experience Rejuvenation Through The Well
• Feature: The Conjuring Meets Little Women: Dordt Professor Releases First Novel; Senior Spotlight; Northwestern Gears Up for NCDC; Professor Natalie Griffioen Joins the Philosophy Department
• Politics: Defenders Vote in their First Presidential Election; Trump's Leapfrog Return; Dordt Students Discuss …

Unlocking Intracellular Oncology Targets: The Unique Role Of Antibody-Based T-Cell Receptor Mimic (Tcrm) Therapeutics In T-Cell Engagers (Tces) And Antibody-Drug Conjugates (Adcs), Jeffrey Molldrem, Dongxing Zha

Faculty, Staff and Student Publications

Effectively targeting intracellular tumor-associated proteins presents a formidable challenge in oncology, as they are traditionally considered inaccessible to conventional antibody-based therapies and CAR-T cell therapies. However, recent advancements in antibody engineering have revolutionized this field, offering promising new strategies to combat cancer. This review focuses on the innovative use of T-cell receptor mimic (TCRm) antibodies within the therapeutic frameworks of T-cell engagers (TCE) and antibody-drug conjugates (ADCs). TCRm antibodies, designed to recognize peptide-MHC complexes rather than cell surface proteins, integrate the capacity of T-cells to reach intracellular targets with the unique strengths of antibodies. When incorporated into T-cell engaging therapeutics, …

Multi-Organ Gene Expression Analysis And Network Modeling Reveal Regulatory Control Cascades During The Development Of Hypertension In Female Spontaneously Hypertensive Rat, Eden Hornung, Sirisha Achanta, Alison Moss, James S. Schwaber, Rajanikanth Vadigepalli

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Hypertension is a multifactorial disease with stage-specific gene expression changes occurring in multiple organs over time. The temporal sequence and the extent of gene regulatory network changes occurring across organs during the development of hypertension remain unresolved. In this study, female spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rats were used to analyze expression patterns of 96 genes spanning inflammatory, metabolic, sympathetic, fibrotic, and renin-angiotensin (RAS) pathways in five organs, at five time points from the onset to established hypertension. We analyzed this multi-dimensional dataset containing ~15,000 data points and developed a data-driven dynamic network model that accounts for …

Plasma Proteome Alterations Of Laying Hens Subjected To Heat Stress And Fed A Diet Supplemented With Pequi Oil (Caryocar Brasiliense Camb.): New Insights In The Identification Of Heat Stress Biomarkers, Joyce Da Silva, Luane Andrade, Paola Rodrigues, Laís Cordeiro, Gabrieli Lima, Júlia Lopes, Elis Castillo, Renata Martins, Andrey Assunção, José Vieira, Marília Busalaf, Jiri Adamec, José Sartori, Pedro Padilha

School of Medicine Faculty Publications

Heat stress can disrupt the balance between the heat poultry release into the environment and the heat they generate. Pequi oil has antioxidant properties, which may mitigate the heat stress effects. This study aimed to investigate the response of laying hens to pequi oil supplementation under heat stress using a proteomic approach. A total of 96 Lohmann White laying hens with 26 weeks old were housed in a completely randomized design with a 2 × 2 factorial arrangement. They were housed in two climate chambers, thermal comfort temperature ± 24.04 °C with the relative humidity ± 66.35 and heat stress …

Microglia Drive Diurnal Variation In Susceptibility To Inflammatory Blood-Brain Barrier Breakdown, Jennifer H. Lawrence, Asha Patel, Melvin W. King, Collin J. Nadarajah, Richard Daneman, Erik S. Musiek

2020-Current year OA Pubs

The blood-brain barrier (BBB) is critical for maintaining brain homeostasis but is susceptible to inflammatory dysfunction. While transporter-dependent efflux of some lipophilic substrates across the BBB shows circadian variation due to rhythmic transporter expression, basal transporter-independent permeability and leakage is nonrhythmic. Whether daily timing influences BBB permeability in response to inflammation is unknown. Here, we induced systemic inflammation through repeated LPS injections either in the morning (ZT1) or evening (ZT13) under standard lighting conditions; we then examined BBB permeability to a polar molecule that is not a transporter substrate, sodium fluorescein. We observed clear diurnal variation in inflammatory BBB permeability, …

Flocs As Vectors For Microplastics In The Aquatic Environment, Nan Wu, Stuart Grieve, Andrew Manning, Kate Spencer

School of Biological and Marine Sciences

No abstract provided.

A Framework To Conceptualize Social Prescribing Services From A Prevention Perspective, Adrienne Alayli, Kerryn Husk, Vashti Berry, Freia De Bock

Peninsula Medical School

Objective: Social prescribing models are expanding worldwide to serve multiple goals, such as attending to social conditions that affect people's health, supporting patients with mental health issues or other long-term conditions, facilitating community building and reducing demands on the health care system. Implicitly, many social prescribing services seek to promote health, prevent morbidity or deterioration of disease. Given that the scientific literature currently does not systematically describe these services as preventive service models, we propose a framework to explicitly conceptualize social prescribing from a prevention perspective. Methods: Based on concepts from prevention science (e.g., classifications of prevention approaches), and a …

Ephb4-Rasa1 Inhibition Of Piezo1 Ras Activation Drives Lymphatic Valvulogenesis, Di Chen, Yipei Tang, Philip E Lapinski, David Wiggins, Eva M Sevick, Michael J Davis, Philip D King

Faculty, Staff and Student Publications

BACKGROUND: EPHB4 (ephrin receptor B4) and the RASA1 (p120 Ras GTPase-activating protein) are necessary for the development of lymphatic vessel (LV) valves. However, precisely how EPHB4 and RASA1 regulate LV valve development is unknown. In this study, we examine the mechanisms by which EPHB4 and RASA1 regulate the development of LV valves.

METHODS: We used LV-specific inducible EPHB4-deficient mice and EPHB4 knockin mice that express a form of EPHB4 that is unable to bind RASA1 yet retains protein tyrosine kinase activity (EPHB4 2YP) to study the role of EPHB4 and RASA1 in LV valve development in the embryo and LV …

Modeling Antisense Oligonucleotide Therapy In Mecp2 Duplication Syndrome Human Ipsc-Derived Neurons Reveals Gene Expression Programs Responsive To Mecp2 Levels, Sameer S Bajikar, Yehezkel Sztainberg, Alexander J Trostle, Harini P Tirumala, Ying-Wooi Wan, Caroline L Harrop, Jesse D Bengtsson, Claudia M B Carvalho, Davut Pehlivan, Bernhard Suter, Jeffrey L Neul, Zhandong Liu, Paymaan Jafar-Nejad, Frank Rigo, Huda Y Zoghbi

Faculty, Staff and Students Publications

Genomic copy-number variations (CNVs) that can cause neurodevelopmental disorders often encompass many genes, which complicates our understanding of how individual genes within a CNV contribute to pathology. MECP2 duplication syndrome (MDS or MRXSL in OMIM; OMIM#300260) is one such CNV disorder caused by duplications spanning methyl CpG-binding protein 2 (MECP2) and other genes on Xq28. Using an antisense oligonucleotide (ASO) to normalize MECP2 dosage is sufficient to rescue abnormal neurological phenotypes in mouse models overexpressing MECP2 alone, implicating the importance of increased MECP2 dosage within CNVs of Xq28. However, because MDS CNVs span MECP2 and additional genes, we generated human …

Tumor-Associated Edema In Children With Kaposi Sarcoma: 14 Years' Experience At Kamuzu Central Hospital, Lilongwe, Malawi, Fatsani Rose Manase, Allison Silverstein, William Kamiyango, Jimmy Villiera, Clement Dziwe, Claudia Wallrauch, Tom Heller, Mark Zobeck, Tamiwe Tomoka, Michael E Scheurer, Carl E Allen, Nmazuo Ozuah, Rizine Mzikamanda, Nader Kim El-Mallawany, Casey L Mcatee

Center for Medical Ethics and Health Policy Staff Publications

Background/objectives: Kaposi sarcoma (KS) is a common lymphatic endothelial cancer among children with and without HIV in central and eastern Africa. Despite its clinical heterogeneity, its various clinical phenotypes are often grouped together in staging and treatment algorithms. Patients with KS tumor-associated edema, referring to hard, non-pitting lesions which often lead to chronic disability, represent a unique, understudied subgroup of children with KS. To continue our work defining the distinct phenotypes of pediatric KS, this study aimed to assess the clinical progression and outcomes of KS edema in children.

Methods: A retrospective cohort study was conducted at Kamuzu Central Hospital …

Dynamic Pricing System For Recommerce Transaction, Madhusudan St

Defensive Publications Series

The present disclosure related to a technique for real-time dynamic pricing recommendations. The technique includes fetching historical data and transactional information related to the plurality of products and corresponding similar products based on queries. The technique further include calculating optimal pricing recommendations based on the fetched historical data and transactional information. Subsequently, the technique include distributing price recommendations for recommerce transaction to the plurality of merchants

Otherness, Survival And Hope: Pedagogies In Popular Media [Dialogue: The Interdisciplinary Journal Of Popular Culture And Pedagogy Volume 6, Issue 3 (December 2019)], Anna S. Cohenmiller

Dialogue: The Interdisciplinary Journal of Popular Culture and Pedagogy

Otherness, Survival and Hope: Pedagogies in Popular Media [Dialogue: The Interdisciplinary Journal of Popular Culture and Pedagogy Volume 6, Issue 3 (December 2019)]

Editorial

Teaching and Learning about Otherness in Popular Culture, Anna S. CohenMiller

Section 1

Learning about Otherness within Formal Education “I am a Conversation”: Media Literacy, Queer Pedagogy, and Steven Universe in College Curriculum, Misty Thomas

Using News to Start Class: How Small Daily Interactions Affect Larger Classroom Interactions, Laura Dumin

Section 2

Informal Learning as Survival “No te voy a dejar nunca”: Culture and Second Language Acquisition for Survival in Fear the Walking Dead, Sharon …

Using News To Start Class: How Small Daily Interactions Affect Larger Classroom Interactions, Laura M. Dumin

Dialogue: The Interdisciplinary Journal of Popular Culture and Pedagogy

In 2013, I added an extra credit assignment to my freshmen composition classes encouraging students to bring in news stories each class period; this assignment was designed to encourage students to be more willing to participate in classroom discussions. We then spent the first few minutes of each class discussing the stories they brought. After using this assignment for a few years, I had anecdotal evidence to suggest that my students were generally more talkative in class after the first week or two of sharing news. These experiences made me want to see if I could measure some change or …

“I Am A Conversation”: Media Literacy, Queer Pedagogy, And Steven Universe In College Curriculum, Misty Thomas

Dialogue: The Interdisciplinary Journal of Popular Culture and Pedagogy

The recent cartoon show on Cartoon Network Steven Universe allows for the blending of both queer theory and media literacies to create a pedagogical space for students to investigate and analyze not only queerness, but also normative and non-normative identities. This show creates characters as well as relationships that both break with and subvert what would be considered traditional masculine and feminine identities. Additionally, Steven Universe also creates a space where sexuality and transgender bodies are represented. This paper demonstrates both the presence of queerness within the show and the pedagogical implications for using this piece of media within a …

Bad Girls: Agency, Revenge, And Redemption In Contemporary Drama, Courtney Watson

Dialogue: The Interdisciplinary Journal of Popular Culture and Pedagogy

Cultural movements including #TimesUp and #MeToo have contributed momentum to the demand for and development of smart, justified female criminal characters in contemporary television drama. These women are representations of shifting power dynamics, and they possess agency as they channel their desires and fury into success, redemption, and revenge. Building on works including Gillian Flynn’s Gone Girl and Netflix’s Orange is the New Black, dramas produced since 2016—including The Handmaid’s Tale, Ozark, and Killing Eve—have featured the rise of women who use rule-breaking, rebellion, and crime to enact positive change.

Nature Vs. Nurture In Albuquerque: What Breaking Bad And Better Call Saul Teach Us About How We Talk About Criminals, Max Romanowski

Dialogue: The Interdisciplinary Journal of Popular Culture and Pedagogy

Breaking Bad and Better Call Saul focus on the criminal transformation of their two main characters, Walter White (Bryan Cranston) and Jimmy McGill (Bob Odenkirk). While quite similar on the surface, Walter and Jimmy’s narratives represent two different criminal transitions, evoking the classic nature vs nurture conversation. Both of these shows bring the conversation to the idea of inevitability. The nature vs. nurture argument is a popular one because it acts as a teaching tool for how we think and talk about criminal behavior. At first, it follows that since criminality was in Walter White’s nature the whole time, his …

Anticancer Activity Of Capparis Spinosa Extract Against Colorectal Cancer, Shamaa Abdul Samad

Thesis/ Dissertation Defenses

Colorectal cancer (CRC) is one of the most frequently diagnosed cancers in the world. Plants are becoming a rich source of new anticancer compounds with novel targets. In this study, we evaluated the anticancer effects of Capparis spinosa on two CRC cell lines. HCT-116 and HT-29 CRC cells treated with various concentrations of C. spinosa leaf extract (CSLE) demonstrated inhibition of proliferation in a concentration-dependent manner. Additional analyses such as Western blot analysis, colony forming assay, and wound healing assay were performed to elucidate the mechanisms and pathways involved. The results revealed that non-cytotoxic concentrations of CSLE significantly inhibited the …

Effects Of Different Warm-Up Protocols On The Cardiopulmonary Responses To Exercise Testing In Youth, Avery D. Faigenbaum, Jie Kang, Jenna Ingui, Aidan Fish, Jessica Dimatteo, Izzy Leazier, Jill Bush, Nicholas Ratamess

International Journal of Exercise Science

International Journal of Exercise Science 17(4): 1530-1539, 2024. The aim of this study was to compare the warm-up effects of a treadmill walking warm-up (TW) with a dynamic warm-up (DW) on the responses to cardiopulmonary exercise testing (CPET) in youth. A sample of 16 active youth (age 13.6 ± 1.8 yr) were tested for peak oxygen uptake (VO₂ peak) using the Fitkids treadmill test protocol on 2 nonconsecutive days following different 6-min warm-up procedures. The TW consisted of walking on a treadmill at 2.2 mph and 0% grade whereas the DW consisted of 12 bodyweight exercises with a 2 …

Using A Rabbit Model To Understand The Impact Of Oral Versus Intravenous Transmission Of Epstein-Barr Virus, Meera Mohammed Al Ameri

Thesis/ Dissertation Defenses

The isolation of Epstein-Barr Virus (EBV) in 1964 from a case of Burkitt lymphoma prompted a surge in research on the biology of this oncogenic virus and its role in the pathogenesis of associated malignancies. Over half a century later, a number of pertinent questions on the dynamics of EBV and the cellular targets of primary infection remain poorly defined. One major obstacle in EBV research has been the lack of a suitable small animal model. EBV is a highly human tropic virus. We have recently established a novel rabbit model of EBV infection that appears to mimic natural infection …

We All Have Jobs Here: Teaching And Learning With Multiple Intelligences In The Walking Dead, Lizabeth Gartley

Dialogue: The Interdisciplinary Journal of Popular Culture and Pedagogy

With the rise of cultural studies, positivism and formalism fell out of favor. But in recent years, altered versions of these methodologies have been suggested as solutions to the deficiencies of the ideological approach dominating the field. Where the ideological approach looks at the content of texts to determine their meaning, the aesthetic approach adopted by media scholars in recent years returns to the close textual readings of formalism (while abandoning its assertion that meaning resides in the text alone). Similarly, where the ideological approach tends to use textual analysis devoid of sociological empiricism, the use of “big data” in …

Assessing The Impact Of Irrigation Water And Soil Salinity On Date Palm And Desert Soil Associated Microbiota, Subha Chandran

Thesis/ Dissertation Defenses

Soil salinization poses a critical challenge in arid and semi-arid regions, where water scarcity is exacerbated by climate change and unsustainable agricultural practices. This phenomenon not only reduces soil fertility but also significantly impacts groundwater quality, leading to detrimental effects on crop yields and ecosystem health. Date palm (Phoenix dactylifera) cultivation, a key agricultural practice in many arid regions, often relies on saline groundwater for irrigation due to limited freshwater resources and low precipitation rates. The use of saline water can create an intricate balance between providing necessary nutrients and introducing harmful salts into the soil. While the role of …

Detection Of Pollutants In Soil Using Laser-Induced Breakdown Spectroscopy (Libs), Ekhlas Jabir Mahmood, Mithaq Muter Mehdy Al- Sultani

Al-Bahir

The tremendous development in various industrial and agricultural fields has led to an increase in pollution of soil and water bodies, which has been reflected in the quality of agricultural products and their quantity, as well as the damage that this pollution causes to human health and the growth system of other living organisms. Many soils are polluted with heavy metal elements like lead (Pb), zinc (Zn), cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), aluminum (Al), and mercury (Hg), which makes the environmental situation more difficult. As part of this sensitive stage, monitoring and analysis have become essential to …

Spartan Daily, November 7, 2024, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2024

Volume 163, Issue 33

Agenda/Program: Frank Reidy Research Center For Bioelectrics Retreat 2024, Frank Reidy Research Center For Bioelectrics, Old Dominion University

2024 Frank Reidy Research Center for Bioelectrics Retreat

Agenda and condensed program for the 2024 Frank Reidy Research Center for Bioelectrics Retreat held on November 7, 2024 at the ODU Priority Club in Norfolk, VA.

41st Annual Reception Bibliography, 2023-2024, Leonard H. Axe Library

Bibliographies

Since 1984, Leonard H. Axe Library at Pittsburg State University has honored book authors and other creators for their outstanding scholarship and contributions to their fields. This bibliography documents creative works published by university faculty and staff within the last year.

Investigating The Role Of Peat Moss In Sage Plant Growth, Lilianna Wrenne, Lindsey Powell, Victoria Rylan Wolkonowski, Fatema Al-Hemoud, Darlene Panvini

Science University Research Symposium (SURS)

This research examined the effect peat moss (Sphagnum flexuosum) had on growth of garden sage (Salvia officinalis). Sage has been used for centuries in traditional medicine for various health benefits. Sage grows best in cooler seasons, but with rising temperatures and longer seasons of heat and humidity, it may be harder to cultivate. Since peat moss helps maintain moisture while fixing carbon and nitrogen levels in the surrounding soil, it might promote healthier sage plant growth. Three sage plants were grown in sheets of peat moss (experimental) and three plants were grown alone (control) on Belmont’s …

A Protocol For Immunostaining Of Macrophages In Whole-Mount Mouse Cornea, Conjunctiva, And Lacrimal Gland, Saleh Alfuraih, Dhruv Patel, Wonsuk Choi, Karthikeyan Ramasamy, Rais Ansari, Ajay Sharma

Pharmacy Faculty Articles and Research

The healthy lacrimal functional unit contains a resident macrophage population. Here, we present a protocol for immunofluorescent staining of macrophage markers, CD11b, F4/80, and CD206, in whole-mount mouse cornea, conjunctiva, and 50-μM-thick lacrimal gland section. We describe steps for dissection, fixation, permeabilization, and blocking. We then detail procedures for the detection and spatial localization of macrophages through immunostaining and confocal imaging. This approach circumvents the need to obtain thin tissue sections and acquire macrophage images from each tissue section.

Identification And Treatment Of Ipv/Nonfatal Strangulation By First Responders, Mary L. Turner

Walden Dissertations and Doctoral Studies

Ineffective and inconsistent education focusing on domestic violence (DV)/intimate partner violence (IPV)/nonfatal strangulation identification and treatments results in first responders’ failure to move patients to a higher level of care, resulting in possible delayed complications. The purpose of the Doctor of Nursing practice (DNP) project was to determine if an educational program increased first responders’ knowledge and improved treatment of DV/IPV/nonfatal strangulation patients. Roger’s diffusion of innovation theory provided the framework throughout the educational process. The Physician Readiness to Manage Intimate Partner Violence Survey (PREMIS) tool was utilized to score the participants’ knowledge prior to and after the education. The …