Digital Commons Network^™

Isolation, Discrimination, And Feeling “Constant Guilt”: A Mixed-Methods Analysis Of Female Physicians’ Experience With Fertility, Family Planning, And Oncology Careers, Sarah Marion, Shraddha M Dalwadi, Aleksandra Kuczmarska-Haas, Erin F Gillespie, Michelle S Ludwig, Emma B Holliday, Bridgette Thom, Fumiko Chino, Anna Lee

Faculty, Staff and Students Publications

Introduction: Family planning among female physicians is harmed by high risks of infertility, workload burden, poor family leave policies, and gender discrimination. Many women report feeling unsupported in the workplace, despite national policies to protect against unfair treatment.

Methods: This secondary analysis applied a modified version of the rigorous and accelerated data reduction technique to conduct a thematic analysis of comments to an open-ended prompt. Comments were coded by multiple trained researchers then grouped and merged into illustrative themes via qualitative techniques.

Results: Of 1004 responses to the quantitative survey, 162 physicians completed the open-ended prompt. Initial codes (n = …

A Phase I First-In-Human Study Of Abbv-011, A Seizure-Related Homolog Protein 6-Targeting Antibody-Drug Conjugate, In Patients With Small Cell Lung Cancer, Daniel Morgensztern, Et Al.

2020-Current year OA Pubs

PURPOSE: Seizure-related homolog protein 6 (SEZ6) is a novel target expressed in small cell lung cancer (SCLC). ABBV-011, a SEZ6-targeted antibody conjugated to calicheamicin, was evaluated in a phase I study (NCT03639194) in patients with relapsed/refractory SCLC. We report initial outcomes of ABBV-011 monotherapy.

PATIENTS AND METHODS: ABBV-011 was administered intravenously once every 3 weeks during dose escalation (0.3-2 mg/kg) and expansion. Patients with SEZ6-positive tumors (≥25% of tumor cells with ≥1+ staining intensity by IHC) were preselected for expansion. Safety, tolerability, antitumor activity, and pharmacokinetics were evaluated.

RESULTS: As of August 2022, 99 patients received ABBV-011 monotherapy [dose escalation, …

Alternatively Spliced Map4 Isoforms Have Key Roles In Maintaining Microtubule Organization And Skeletal Muscle Function, Lathan Lucas, Larissa Nitschke, Brandon Nguyen, James A Loehr, George G Rodney, Thomas A Cooper

Faculty, Staff and Students Publications

Skeletal muscle cells (myofibers) are elongated non-mitotic, multinucleated syncytia that have adapted a microtubule lattice. Microtubule-associated proteins (MAPs) play roles in regulating microtubule architecture. The most abundant MAP in skeletal muscle is MAP4. MAP4 consists of a ubiquitous MAP4 isoform (uMAP4), expressed in most tissues, and a striated-muscle-specific alternatively spliced isoform (mMAP4) that includes a 3,180-nucleotide exon (exon 8). To determine the role of mMAP4 in skeletal muscle, we generated mice that lack mMAP4 and express only uMAP4 due to genomic deletion of exon 8. We demonstrate that loss of mMAP4 leads to disorganized microtubule architecture and intrinsic loss of …

Sample Multiplexing For Retinal Single-Cell Rna Sequencing, Justin Ma, Ting-Kuan Chu, Maria Polo-Prieto, Yong H Park, Yumei Li, Rui Chen, Graeme Mardon, Benjamin J Frankfort, Nicholas M Tran

Faculty, Staff and Students Publications

Rare cell populations can be challenging to characterize using microfluidic single-cell RNA sequencing (scRNA-seq) platforms. Typically, the population of interest must be enriched and pooled from multiple biological specimens for efficient collection. However, these practices preclude the resolution of sample origin together with phenotypic data and are problematic in experiments in which biological or technical variation is expected to be high (e.g., disease models, genetic perturbation screens, or human samples). One solution is sample multiplexing whereby each sample is tagged with a unique sequence barcode that is resolved bioinformatically. We have established a scRNA-seq sample multiplexing pipeline for mouse retinal …

Going Against The Family: Perturbation Of A Greenbeard Pathway Leads To Falsebeard Cheating, Peter Lehmann, Mariko Katoh-Kurasawa, Peter Kundert, Gad Shaulsky

Faculty, Staff and Students Publications

Greenbeards facilitate cooperation by encoding a perceptible signal, the ability to detect it, and a tendency to help others that display it. Falsebeards are hypothetical cheaters that display the signal without being altruistic. Despite many examples of greenbeards, evidence for falsebeards is scarce. The Dictyostelium discoideum tgrB1-tgrC1 allorecognition pathway encodes a greenbeard. It allows development, which yields fruiting bodies with altruistic stalks that increase spore dispersal. Here we show that cells lacking rapgapB, a tgrB1-tgrC1 signaling element, cheat by avoiding the stalk fate and generating more spores in chimeras than in pure populations. rapgapB– cells cheat only on …

Clinical Outcomes Of Isoniazid Preventive Therapy Amongst Persons Living With Hiv Attending A Referral Hospital: A Cross-Sectional Study, Mwaka Dick Odong, Wafula Bossa, Muhwezi Loyce, Nangobi Christine Faith, Mbusa Chrispus, Bontu Aschale Abebe, Daniel Chans Mwandah, Silas Ojuka, Tadele Mekuriya Yadesa

AUIQ Complementary Biological System

Tuberculosis (TB) is the most common opportunistic infection amongst persons living with HIV (Human Immunodeficiency Virus) (PLWHIV). World Health Organisation recommends tuberculosis preventive therapy (TPT) to avert the effects of TB/HIV co-infection. This study aimed at determining the incidence of active tuberculosis infection, assessing the burden of adverse drug events (ADEs) and identifying the associated factors. In this study, records of 379 patients who started isoniazid preventive therapy at Mbarara Regional Referral Hospital (MRRH) between 1^st July, 2017 and 30^th June, 2022 were retrospectively reviewed. Data was analyzed using a statistical software (SPSS version 20.0) and presented using …

Pulmonary Alveolar Microlithiasis And Interstitial Pneumonitis: A Case Report Of The West Of Iran, Mazaher Ramezani, Zahra Aminparast, Masoud Sadeghi

BioMedicine

Pulmonary alveolar microlithiasis (PAM) is a rare disease with autosomal recessive inheritance. Herein, a 20-year-old lady referred to the hospital with a dry cough for two years. The chest X-ray findings were bilateral reticulonodular opacities in both lungs and honeycomb appearance suspicious for miliary tuberculosis and idiopathic pulmonary fibrosis. A wedge biopsy of lung showed that there were several intraalveolar laminated concretions in the pathology report compatible with pulmonary alveolar microlithiasis and interstitial infiltration of lymphocytes and neutrophils compatible with interstitial pneumonitis. PAM is a rare progressive disease with the production of microliths in pulmonary alveoli. The pathologist, radiologist, and …

Epigenetic Profiling Of Mutyh, Klf6, Wnt1 And Klf4 Genes In Carcinogenesis And Tumorigenesis Of Colorectal Cancer, Kosar Babaei, Roya Khaksar, Tahereh Zeinali, Hossein Hemmati, Ahmadreza Bandegi, Pirouz Samidoust, Mohammad Taghi Ashoobi, Hooman Hashemian, Kourosh Delpasand, Fereshteh Talebinasab, Hoora Naebi, Seyed Hossein Mirpour, Arman Keymoradzadeh, Seyedeh Elham Norollahi

BioMedicine

Colorectal cancer (CRC) is distinguished by epigenetic elements like DNA methylation, histone modification, histone acetylation and RNA remodeling which is related with genomic instability and tumor initiation. Correspondingly, as a main epigenetic regulation, DNA methylation has an impressive ability in order to be used in CRC targeted therapy. Meaningly, DNA methylation is identified as one of most important epigenetic regulators in gene expression and is considered as a notable potential driver in tumorigenesis and carcinogenesis through gene-silencing of tumor suppressors genes. Abnormal methylation situation, even in the level of promoter regions, does not essentially change the gene expression levels, particularly …

The Rna Degradation Pathway Is Involved In Pparα-Modulated Anti-Oral Tumorigenesis, Nai-Wen Chang, Yi-Ping Huang

BioMedicine

Background: The activation of peroxisome proliferator-activated receptor alpha (PPARα) has been shown to reprogram tumor metabolism and exhibits great potential for treating anti-oral tumorigenesis.

Methods: In this study, we used a pathway-based strategy to explore possible functional pathways involved in the anticancer activity of PPARα in oral cancer cells through next-generation sequencing (NGS) and bioinformatic approaches.

Results: We found that 3919 genes were upregulated and 1060 genes were downregulated through PPARα activation. These genes were mainly involved in the proteasomal, mRNA surveillance, spliceosomal, RNA transport, and RNA degradation pathways, as indicated by GO and KEGG enrichment analysis. Importantly, a total …

Oral Acute And Sub-Acute Toxic Effects Of HydroalcoholicTerminalia ChebulaRetz AndAchillea WilhelmsiiExtracts In Balb/C Mice, Mahnaz Jafari, Kourosh Manochehri Naeini, Zahra Lorigooini, Rasool Namjoo

BioMedicine

Background: This study examined the acute and sub-acute toxic effects ofTerminalia chebulaandAchillea wilhelmsiiextracts on the murine model.

Methods: In both phases, mice were assigned to intervention and control groups. At the end of study, the liver, kidney, and heart tissues were collected for histopathological studies.

Results: In the acute phase of the study, the safe dose was ≤5000 mg/kg for both extracts. In sub-acute phase, LD50 (95% CI) ofAchillea wilhelmsiiextract was determined ≥5000 mg/kg and that ofTerminalia chebulaextract 2754.436 (2438-3114) mg/kg. The highest dose ofT. chebulaextract induced few histopathological changes.

Conclusion: It will be useful to gain information on the …

The Potential Therapeutic Effect For Melatonin And Mesenchymal Stem Cells On Hepatocellular Carcinoma, Yasser Mohamed, Mohamed A. Basyony, Nabila I. El-Desouki, Walied S. Abdo, Mohammed A. El-Magd

BioMedicine

Background/aim: Herein, we investigated the potential therapeutic effect of Melatonin (Mel) and/or mesenchymal stem cells (MSCs) on rat model of HCC.

Materials and Methods: Female mature rats were divided into 5 groups (n= 10/group): normal (Nor), HCC group intraperitoneally injected with 200 mg/kg DEN, and 3 treated groups; HCC + Mel (Mel) group given Mel intraperitoneally 20 mg/kg, twice a week, HCC + MSCs (MSCs) group intravenously injected by 1 × 10⁶ cells, and HCC + MSCs (Mel +MSCs) group.

Results: Rats in HCC group showed most deteriorated effect in form of increased mortality and relative liver weight, elevated …

Immunohistochemical Expression Of P53, Ki-67, And Cd34 In Psoriasis And Psoriasiform Dermatitis, Mazaher Ramezani, Atefeh Shamshiri, Elisa Zavattaro, Sedigheh Khazaei, Mansour Rezaei, Rozhano Mahmoodi, Masoud Sadeghi

BioMedicine

Background: Psoriasis is the prime example of psoriasiform tissue pattern and should be differentiated from other psoriasiform dermatoses both clinically and histopathologically.

Aim: To evaluate immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis for diagnostic purposes.

Methods: An analytical cross-sectional study was performed on the paraffin blocks of 60 psoriasis and 31 psoriasiform dermatitis patients between 2014 and 2017. The selected formalin-fixed paraffin-embedded tissues from each biopsy specimen were cut into 4-micron sections. Initial sections were stained by hematoxylin and eosin staining. Primary antihuman antibodies against P53, Ki-67, and CD34 were applied. Positive control samples for …

Cohort Study Of Microelement Status In “Healthy” Population Of Russian Megapolis, Andrew K. Martusevich, Konstantin A. Karuzin

BioMedicine

The aim of the study was at the cohort assessment of microelement status in large city residents classified as “apparently healthy people”. The population study included 2,025 randomly selected middle-aged (2045 years) persons without chronic diseases or acute infectious pathologies. The set of subjects was standardized by age and gender. A blood sample was taken once from each person to determine concentrations of microelements. The level of peripheral blood microelements were determined by atomic absorption spectrometry using «Shimadzu AA7000» device (Japan). The study of a large population of city residents demonstrated inhomogeneity in their microelement status. Deficit conditions were found …

Evaluation Of The Serum Β2 Microglobulin Level In Patients With Systemic Lupus Erythematosus And Its Correlation With Disease Activity, Miramir Aghdashi, Simak Salami, Ahmad Nezhadisalami

BioMedicine

Background: Designation of disease activity is serious for the management of systemic lupus erythematosus (SLE). Serum level of β2 microglobulin (β2M) may be associated with illness activity in SLE disease. Since the role of β2M for assessing of illness activity in SLE is not completely clear, the current study aimed to discern evaluation of β2M in patients with SLE and its correlation with sickness activity.

Materials and Methods: In this case-control study, 50 patients with SLE disease and 25 healthy individuals were selected in Imam Khomeini Hospital in central of Urmia.

Blood samples were collected safely from patients, serum was …

Development Of Broad Spectrum Mycosporine Loaded Sunscreen Formulation From Ulva Fasciata Delile, Saurabh Bhatia, Satish Sardana, Ajay Sharma, Celia Bertha Vargas De La Cruz, Bhupal Chaugule, Laleh Khodaie

BioMedicine

Ba.ckground: Sunscreen formulations primarily offer protection against UV induced damages however nowadays it also maintains skin natural physiological conditions. Current global market is flooded with numerous sunscreen products which offer protection to skin against several UV induced damages. However most of these sunscreen formulations offers narrow spectrum protection against UV and also suffer from stability as well as toxicity related issues.

Methods: Present work aims to isolate mycosporine amino acid (Mgy) from green alga namelyUlva fasciata (U. fasciata)and study its sunscreen potential against widely used domestic marketed formulation. Stability evaluations were also performed for almost 90 days.

Results: Results demonstrated …

Fascioliasis Mimicking Malignancy In An Old Patient, Mazaher Ramezani, Farhad Amirian, Masoud Sadeghi

BioMedicine

No abstract provided.

Protective Effect OfGlycyrrhiza GlabraRoots Extract On Bone Mineral Density Of Ovariectomized Rats, Dimitrios Galanis, Konstantinos Soultanis, Pavlos Lelovas, Alexandros Zervas, Panagiotis Papadopoulos, Antonis Galanos, Katerina Argyropoulou, Maria Makropoulou, Anastasia Patsaki, Christina Passali, Anastasia Tsingotjidou, Stavros Kourkoulis, Sofia Mitakou, Ismene Dontas

BioMedicine

Objective: The aim of this study was to evaluate the potential effect of the methanolic extract of plantGlycyrrhiza glabraroots on bone mineral density and femoral bone strength of ovariectomized rats.

Methods: Thirty 10-month-old Wistar rats were randomly separated into three groups of ten, Control, Ovariectomy and Ovariectomy-plus-Glycyrrhiza in their drinking water. Total and proximal tibial bone mineral density was measured in all groups before ovariectomy (baseline) and after 3 and 6 months post ovariectomy. Three-point-bending of the femurs and uterine weight and histology were examined at the end of the study.

Results: No significant difference was noted in bone density …

In VitroAntioxidant And Antinociceptive Properties OfPorphyra Vietnamensis, Saurabh Bhatia, Satish Sardana, Kishan Ram Senwar, Anjali Dhillon, Ajay Sharma, Tanveer Naved

BioMedicine

No abstract provided.

Materials And Structures Used In Meniscus Repair And Regeneration: A Review, Ketankumar Vadodaria, Abhilash Kulkarni, E Santhini, Prakash Vasudevan

BioMedicine

No abstract provided.

A Brief Literature Review And Own Clinical Experience In Prophylaxis Of Oral Mucositis In Children Using Low Level Laser Therapy, Sergey Moskvin, Denis Pritiko, Elena Sergeenko, Elena Lukash, Leonid Gusev

BioMedicine

No abstract provided.

Chromophobe Renal Cell Carcinoma Or Oncocytoma: A Manner Of Challenge In Frozen Section Diagnosis, Farideh Baharzadeh, Masoud Sadeghi, Mazaher Ramezani

BioMedicine

No abstract provided.

The Effect Of Selenium Supplementation On Cystine Crystal Volume In Patients With Cystinuria, Mehrdad Mohammadi, Amin Shohani, Hatef Khorami, Kia Nouri Mahdavi, Mohammad Hossein Izadpanahi, Farshid Alizadeh, Mohammad Azizi

BioMedicine

No abstract provided.

Study Of The Effect Of The Essential Oil (Extract) Of Rhubarb Stem (Shoot) On Glycosylated Hemoglobin And Fasting Blood Glucose Levels In Patients With Type Ii Diabetes, Fahimeh Shojaei Shad, Maryam Jahantigh Haghighi

BioMedicine

No abstract provided.

A Comparison Of The Effects Of Entonox Inhalation And Spinal Anesthesia On Labor Pain Reduction And Apgar Score In Vaginal Delivery: A Clinical Trial Study, Samira Foji, Manijeh Yousefi Moghadam, Hosein Tabasiasl, Milad Nazarzadeh, Hamid Salehiniya

BioMedicine

No abstract provided.

Cervical Cancer In Iran: Integrative Insights Of Epidemiological Analysis, Zohre Momenimovahed, Hamid Salehiniya

BioMedicine

No abstract provided.

Benzyl Isothiocyanate (Bitc) Triggers Mitochondria-Mediated Apoptotic Machinery In Human Cisplatin-Resistant Oral Cancer Car Cells, Chiu-Fang Lee, Ni-Na Chiang, Yao-Hua Lu, Yu-Syuan Huang, Jai-Sing Yang, Shih-Chang Tsai, Chi-Cheng Lu, Fu-An Chen

BioMedicine

No abstract provided.

Mechanical Stretch And Chronotherapeutic Techniques For Progenitor Cell Transplantation And Biomaterials, Eve Helena Rogers, Vanja Pekovic-Vaughan, John Alan Hunt

BioMedicine

No abstract provided.

A Screening Of Growth Inhibitory Activity Of Iranian Medicinal Plants On Prostate Cancer Cell Lines, Majid Asadi-Samani, Mahmoud Rafieian-Kopaei, Zahra Lorigooini, Hedayatollah Shirzad

BioMedicine

No abstract provided.

Effectiveness Of Low Level Laser Therapy For Treating Male Infertility, Sergey Vladimirovich Moskvin, Oleg Ivanovich Apolikhin

BioMedicine

No abstract provided.

The Relationship Between Health-Promoting Lifestyle And Sleep Quality In Postmenopausal Women, Asieh Moudi, Ali Dashtgard, Hamid Salehiniya, Maryam Sadat Katebi, Mohammad Reza Razmara, Mohammad Reza Jani

BioMedicine

No abstract provided.