Digital Commons Network^™

Screams In The Woods: Friendship And Forbidden Secrets, Jade Bauer

Philomathean

No abstract provided.

A Mourning Glory, Zoe Martin

Philomathean

No abstract provided.

The Mad Scientist, Melissa Buckley

Philomathean

No abstract provided.

Single-Molecule Studies Reveal The Off-Pathway Early Paused State Intermediates As A Target Of Streptolydigin Inhibition Of Rna Polymerase And Its Dramatic Enhancement By Gre Factors, Anatolii Arseniev, Mikhail Panfilov, Georgii Pobegalov, Alina Potyseva, Polina Pavlinova, Maria Yakunina, Jookyung Lee, Sergei Borukhov, Konstantin Severinov, Mikhail Khodorkovskii

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Antibiotic streptolydigin (Stl) inhibits bacterial transcription by blocking the trigger loop folding in the active center of RNA polymerase (RNAP), which is essential for catalysis. We use acoustic force spectroscopy to characterize the dynamics of transcription elongation in ternary elongation complexes (ECs) of RNAP in the presence of Stl at a single-molecule level. We found that Stl induces long-lived stochastic pauses while the instantaneous velocity of transcription between the pauses is unaffected. Stl enhances the short-lived pauses associated with an off-pathway early paused state intermediates of the RNAP nucleotide addition cycle. Unexpectedly, we found that transcript cleavage factors GreA and …

Rna Splicing As A Biomarker And Phenotypic Driver Of Meningioma Dna-Methylation Groups., Nathan Leclair, Abrar Choudury, William C Chen, Stephen T Magill, Kathleen Mccortney, Craig M Horbinski, Zhenhong Chen, Ezequiel Goldschmidt, Charlotte D Eaton, Ketan R Bulsara, Wenya Linda Bi, Akash J Patel, Felix Sahm, David Raleigh, Olga Anczuków

Faculty Research 2024

BACKGROUND: Advances in our understanding of the molecular biology of meningiomas have led to significant gains in the ability to predict patient prognosis and tumor recurrence and to identify novel targets for therapeutic design. Specifically, classification of meningiomas based on DNA methylation has greatly improved our ability to risk stratify patients, however new questions have arisen in terms of the underlying impact these DNA-methylation signatures have on meningioma biology.

METHODS: This study utilizes RNA-sequencing data from 486 meningioma samples corresponding to 3 meningioma DNA-methylation groups (merlin-intact, immune-enriched, and hypermitotic), followed by in vitro experiments utilizing human meningioma cell lines.

RESULTS: …

Little Shop Of Patriarchy : Analyzing Constructed Vs. Essential Gender Roles, Taylor Gadsden-Deterville

Student Scholar Symposium Abstracts and Posters

Frank Oz’s Little Shop of Horrors (1986) is more than a silly kooky musical. The comedic plot dramatically dances with themes of domestic violence, poverty, and sexism. For this project, my poster will explore the expectations and representations of femininity and masculinity set up at the film's beginning versus the end. The film’s context will connect to research involving gender essentialism (innate, biological sex) and gender constructionism (socially constructed gender roles). I will analyze the roles of Seymour, Audrey, and Orin to exhibit how they discover their constructed and essential gender identities. The film represents Audrey as a ditsy hyper-feminine …

Medical School Watercooler Newsletter - December 5, 2024, Lindsay Hughes

Watercooler Newsletter

This is the Thursday, December 5, 2024 edition of the Frederick P. Whiddon College of Medicine's newsletter -- Watercooler.

Contents include:

• Students, faculty present research at international meeting on tropical medicine
• Medical students present research on peer-to-peer learning at national conference
• USA Health Pediatrics expands with addition of Weidow
• Pierce elected chair of APQI Steering Committee
• Creg named director of Office of Continuing Medical Education
• Dasgupta receives 2024 Mayer Mitchell Award for Excellence in Cancer Research
• Pathology resident presents research on melanoma at national dermapathology conference
• USA Health welcomes Benjamin to infectious disease division

Butterfly Peacock Bass (Cichla Ocellaris), Samuel K. Zellefrow, David Kerstetter, John Nichols

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Fall 2024 Student Scholar Symposium Abstract Volume, Chapman University Center For Undergraduate Excellence

Student Scholar Symposium Abstracts and Posters

No abstract provided.

Digital Screen Media Use, Movement Behaviors, And Child Health, Mark S. Tremblay, Nicholas Kuzik, Stuart J.H. Biddle, Valerie Carson, Mai J.M. Chinapaw, Dorothea Dumuid, Wendy Yajun Huang, Travis J. Saunders, Amanda E. Staiano, Russell R. Pate

Population and Public Health Science Faculty Publications

This chapter summarizes the associations between children's digital screen media use (DSMU) and their health, within the 24-h movement behavior framework (physical activities, sedentary behaviors, sleep), provides recommendations for healthy DSMU, and highlights future research directions. Key concepts include behavior displacement, combined associations, and DSMU context and content. Displacement examples include more DSMU decreasing time for healthier behaviors (e.g., physical activity or sleep) or replacing reading books and magazines with DSMU alternatives (e.g., texting, social media). How DSMU affects the relationships between various combinations of movement behaviors and children's health is largely unknown. Total DSMU is most frequently studied, but …

Whose Information Is Whose? Adult Adoptees And The Silent Struggle To Access Records, Alyssa Traina

Undergraduate Honors Theses

The United States has fifty-one different laws pertaining to adult adoptee access to their original birth certificates and adoption court records, with only fifteen states being unrestricted. These states give adult adoptees access to their documents upon request. Fifteen states are restricted, meaning they require a court order to obtain documents, which involves proving “good cause” to a judge. This requirement of “good cause” has no standard definition. The remaining twenty-one states are compromised, meaning they have complex requirements to obtain documentation. My research uses case studies of the restricted laws of California, the unrestricted laws of Louisiana, and the …

Comparisons Of Buried And Unburied Benthic Communities On Mesophotic Coral Ecosystems, Michael Mcdonough

All HCAS Student Capstones, Theses, and Dissertations

Mesophotic coral ecosystems, MCEs, are coral ecosystems found at depths between 30-150m and are divided into two zones-the upper and lower MCE. The upper MCE serves as a transitional zone between shallow water reefs (SWRs) and the lower MCE. This transition is marked by increased reliance on heterotrophy by benthic organisms. While MCEs have been of increased interest by resource managers as potential sources of resiliency for impacted benthic species, these benthic communities are understudied. Mesophotic benthic communities in close proximity of Port Everglades between 32-37m depth were surveyed using video footage recorded by a remotely operated vehicle (ROV). This …

Progerin Can Induce Dna Damage In The Absence Of Global Changes In Replication Or Cell Proliferation, Liza A. Joudeh, P. Logan Schuck, Nina M. Van, Alannah J. Dicintio, Jason A. Stewart, Alan S. Waldman

Faculty Publications

Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare genetic condition characterized by features of accelerated aging, and individuals with HGPS seldom live beyond their mid-teens. The syndrome is commonly caused by a point mutation in the LMNA gene which codes for lamin A and its splice variant lamin C, components of the nuclear lamina. The mutation causing HGPS leads to production of a truncated, farnesylated form of lamin A referred to as "progerin." Progerin is also expressed at low levels in healthy individuals and appears to play a role in normal aging. HGPS is associated with an accumulation of genomic DNA …

Investigating The Relationship Between Population Density Surrounding An Airport And The Number Of Wildlife Strikes Per Operation., Alexandra E. Rowe, Daniel Alves, Evan Park, Brooke Wheeler, Vivek Sharma

Aeronautics Student Publications

This study compared the relationship between population density surrounding an airport and reported wildlife strikes per operation. A very weak correlation with no statistical significance was determined which emphasizes the need for future research on wildlife mitigation strategies and understanding wildlife patterns.

Wildflowers A Novel, Kelli Roberts

Theses

Abstract for Wildflowers

Wildflowers is a feminist literary novel that examines the intersection of sex, power, and free will, exploring how these forces shape and define human agency. The story follows a group of women drawn to an enigmatic leader who promises enlightenment and empowerment through embracing their desires. As they navigate the fine line between self-actualization and manipulation, the novel delves into themes of liberation, coercion, and the choices we make to create the lives we long for. Provocative and thought-provoking, Wildflowers invites readers to consider the complexities of desire, the cost of empowerment, and the courage required to …

Effect Of Laser Textured Surface With Different Patterns On Tribological Characteristics Of Bearing Material, Paul Joshua S

Theses and Dissertations

This research work determines the wear rate and friction characteristics of bearing material under different laser textured surface conditions. Chrome steels are used in bearings since they possess high strength and wear resistance. However, when those parts are in service, failure happens due to sliding friction before the lifetime. To improve the durability of the American Iron and Steel Institute (AISI) 52100 chromium steel, in this work, the effect of laser surface texturing (LST) was analysed.

With the different pattern of circle, ellipse, groove and grid were produced on the sample surface, the wear behavior was investigated using the pin …

Species Detection And Discovery Of Zoanthidea Symbiotic With Deep-Sea Gorgonians, Saige Jost

All HCAS Student Capstones, Theses, and Dissertations

Symbiotic marine invertebrates known as Zoanthidea, from the phylum Cnidaria, live on a wide diversity of other organisms. Deep-sea corals such as Gorgonians are one of these clades of hosts. These octocorals and their Zoanthidean symbionts are challenging to collect and consequently difficult to study. However, the Evolution of Marine Symbioses Laboratory at Nova Southeastern University has obtained several samples from the Yale Peabody Museum and other museums or universities with the goal of expanding our knowledge of the diversity of Zoanthideans. Until recently, the existing taxonomic system has been based on morphological characters. A competing molecular parataxonomic system has …

Faculty Senate Roster 2024-2025, Faculty Senate

Faculty Senate Documents

A list distributed by Faculty Senate of members of the governance body and affiliated committees.

Effect Of Polyacrylamide Gel Elasticity On Collagen Type Ii Fibril Assembly, Kathryn G. Wilcox, Stephanie Kramer, Surajit Chatterjee, Adam Linscott, Sneha Suresh, Lydia Kisley, Svetlana Morozova

Faculty Scholarship

Collagen type II fibrils provide structural integrity to the articular cartilage extracellular matrix. However, the conditions that control the fibril radial size scale, distribution, and formation inside of dense networks are not well understood. We have investigated how surrounding elastic networks affect fibril formation by observing the structure and dynamics of collagen type II in model polyacrylamide gels of varying moduli. Cryogenic transmission electron microscopy (cryo-TEM) is used to image the fibril structure and is verified qualitatively with optical microscopy of fluorescently-tagged collagen within the gels. Using fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI), the diffusion dynamics of the …

Ices Roadmap For Marine Recreational Fisheries (Mrf), International Council For The Exploration Of The Sea (Ices), Karina L. Ryan

Fisheries Research Articles

Purpose of the roadmap

The overarching goal of this roadmap is to guide the development of robust marine recreational fisheries (MRF) assessments through data and methodological improvements, in line with current and future management needs. To address the requirements of the policy and legislative instruments in relation to MRF and to plan future steps in ICES, there is need to further improve and operationalize the required MRF dataflows and assessment procedures. Better integration of MRF into advice is in line with ICES vision and mission alongside its advisory framework and principles. This will assist not only managers but also the …

Montana Kaimin, December 5, 2024, Students Of The University Of Montana, Missoula

Montana Kaimin, 1898-present

Student newspaper of the University of Montana, Missoula.

The Canada Disability Benefit Act And Women With Disabilities: Pursuing Disability Equality And Reducing Administrative Violence, Laverne Jacobs

Osgoode Hall Law Journal

Stakeholders have welcomed the Canadian Disability Benefits Act as an opportunity to rectify the long-standing socioeconomic disadvantage experienced by persons with disabilities. This article examines one specific form of marginalization that lies beneath the surface of the discussions surrounding poverty and persons with disabilities in Canada: It considers women with disabilities and disabled women from intersecting backgrounds, who face unique experiences of poverty that need to be addressed by any legislation aiming to eradicate income barriers for the disability community. By drawing on the theoretical frameworks of bureaucratic disentitlement, administrative violence, and disability equality, this article examines the lived realities …

Liar-Dependent Gene Expression Contributes To Antimicrobial Responses In Group A, Luis Alberto Vega, Misú Sanson-Iglesias, Piyali Mukherjee, Kyle D Buchan, Gretchen Morrison, Anne E Hohlt, Anthony R Flores

Faculty, Staff and Student Publications

The ability to sense and respond to host defenses is essential for pathogen survival. Some mechanisms involve two-component systems (TCSs) that respond to host molecules, such as antimicrobial peptides (AMPs), and activate specific gene regulatory pathways to aid in survival. Alongside TCSs, bacteria coordinate cell division proteins, chaperones, cell wall sortases, and secretory translocons at discrete locations within the cytoplasmic membrane, referred to as functional membrane microdomains (FMMs). In group A Streptococcus (GAS), the FMM or “ExPortal” coordinates protein secretion, cell wall synthesis, and sensing of AMP-mediated cell envelope stress via the LiaFSR three-component system. Previously, we showed that GAS …

Increased Spatial Coupling Of Integrin And Collagen Iv In The Immunoresistant Clear-Cell Renal-Cell Carcinoma Tumor Microenvironment., Alex C. Soupir, Mitchell T. Hayes, Taylor C. Peak, Oscar Ospina, Nicholas H. Chakiryan, Anders E. Berglund, Paul A. Stewart, Jonathan Nguyen, Carlos Moran Segura, Natasha L. Francis, Paola M Ramos Echevarria, Jad Chahoud, Roger Li, Kenneth Y. Tsai, Jodi A. Balasi, Yamila Caraballo Peres, Jasreman Dhillon, Lindsey A. Martinez, Warren E. Gloria, Nathan Schurman, Sean Kim, Mark Gregory, James Mulé, Brooke L. Fridley, Brandon J. Manley

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Immunotherapy has improved survival for patients with advanced clear cell renal cell carcinoma (ccRCC), but resistance to therapy develops in most patients. We use cellular-resolution spatial transcriptomics in patients with immunotherapy naïve and exposed primary ccRCC tumors to better understand immunotherapy resistance.

RESULTS: Spatial molecular imaging of tumor and adjacent stroma samples from 21 tumors suggests that viable tumors following immunotherapy harbor more stromal CD8 + T cells and neutrophils than immunotherapy naïve tumors. YES1 is significantly upregulated in immunotherapy exposed tumor cells. Spatial GSEA shows that the epithelial-mesenchymal transition pathway is spatially enriched and the associated ligand-receptor transcript …

Clonal Landscape And Clinical Outcomes Of Telomere Biology Disorders: Somatic Rescue And Cancer Mutations, Fernanda Gutierrez-Rodrigues, Emma M Groarke, Natthakan Thongon, Juan Jose Rodriguez-Sevilla, Luiz Fernando B Catto, Marena R Niewisch, Ruba Shalhoub, Lisa J Mcreynolds, Diego V Clé, Bhavisha A Patel, Xiaoyang Ma, Dalton Hironaka, Flávia S Donaires, Nina Spitofsky, Barbara A Santana, Tsung-Po Lai, Lemlem Alemu, Sachiko Kajigaya, Ivana Darden, Weiyin Zhou, Paul V Browne, Subrata Paul, Justin Lack, David J Young, Courtney D Dinardo, Abraham Aviv, Feiyang Ma, Michel Michels De Oliveira, Ana Paula De Azambuja, Cynthia E Dunbar, Malgorzata Olszewska, Emmanuel Olivier, Eirini P Papapetrou, Neelam Giri, Blanche P Alter, Carmem Bonfim, Colin O Wu, Guillermo Garcia-Manero, Sharon A Savage, Neal S Young, Simona Colla, Rodrigo T Calado

Faculty, Staff and Student Publications

Telomere biology disorders (TBDs), caused by pathogenic germ line variants in telomere-related genes, present with multiorgan disease and a predisposition to cancer. Clonal hematopoiesis (CH) as a marker of cancer development and survival in TBDs is poorly understood. Here, we characterized the clonal landscape of a large cohort of 207 patients with TBD with a broad range of age and phenotype. CH occurred predominantly in symptomatic patients and in signature genes typically associated with cancers: PPM1D, POT1, TERT promoter (TERTp), U2AF1S34, and/or TP53. Chromosome 1q gain (Chr1q+) was the commonest karyotypic abnormality. Clinically, multiorgan involvement and CH in TERTp, TP53, …

Integrative Multi-Omics Analysis Uncovers Tumor-Immune-Gut Axis Influencing Immunotherapy Outcomes In Ovarian Cancer, Spencer R Rosario, Mark D Long, Shanmuga Chilakapati, Eduardo Cortes Gomez, Sebastiano Battaglia, Prashant K Singh, Jianmin Wang, Katy Wang, Kristopher Attwood, Suzanne M Hess, A J Robert Mcgray, Kunle Odunsi, Brahm H Segal, Gyorgy Paragh, Song Liu, Jennifer A Wargo, Emese Zsiros

Faculty, Staff and Student Publications

Recurrent ovarian cancer patients, especially those resistant to platinum, lack effective curative treatments. To address this, we conducted a phase 2 clinical trial (NCT02853318) combining pembrolizumab with bevacizumab, to increase T cell infiltration into the tumor, and oral cyclophosphamide, to reduce the number of regulatory T cells. The trial accrued 40 heavily pretreated recurrent ovarian cancer patients. The primary endpoint, progression free survival, was extended to a median of 10.2 months. The secondary endpoints demonstrated an objective response rate of 47.5%, and disease control in 30% of patients for over a year while maintaining a good quality of life. We …

Natural Medicine Use In Costa Rica, Elizabeth Krol

Honors Theses

This study explores the role of natural medicine in Costa Rican culture, while focusing on its historical, cultural, and clinical significance. With a literature review, ethnobotanical research, and a study abroad experience, this paper highlights the integration of natural, herbal medicine into modern healthcare. Costa Rica’s rich biodiversity, with its varied microclimates and abundant medicinal plants, forms a strong foundation for traditional herbal medicine. Indigenous groups such as the BriBri and Cabécar contributed significantly to this legacy, which has evolved through interactions with European and Afro-Caribbean populations.

Research emphasizes the cultural importance of herbal medicine within the “Pura Vida” lifestyle, …

Spatial Dynamics Of Culex Quinquefasciatus Abundance: Geostatistical Insights From Harris County, Texas, Morgan Jibowu, Melissa S Nolan, Ryan Ramphul, Heather T Essigmann, Abiodun O Oluyomi, Eric L Brown, Maximea Vigilant, Sarah M Gunter

Faculty, Staff and Students Publications

Mosquito-borne diseases pose a significant public health threat, prompting the need to pinpoint high-risk areas for targeted interventions and environmental control measures. Culex quinquefasciatus is the primary vector for several mosquito-borne pathogens, including West Nile virus. Using spatial analysis and modeling techniques, we investigated the geospatial distribution of Culex quinquefasciatus abundance in the large metropolis of Harris County, Texas, from 2020 to 2022. Our geospatial analysis revealed clusters of high mosquito abundance, predominantly located in central Houston and the north-northwestern regions of Harris County, with lower mosquito abundance observed in the western and southeastern areas. We identified persistent high mosquito …

Rna Splicing As A Biomarker And Phenotypic Driver Of Meningioma Dna-Methylation Groups, Nathan K Leclair, Abrar Choudury, William C Chen, Stephen T Magill, Kathleen Mccortney, Craig M Horbinski, Zhenhong Chen, Ezequiel Goldschmidt, Charlotte D Eaton, Ketan R Bulsara, Wenya Linda Bi, Akash J Patel, Felix Sahm, David Raleigh, Olga Anczukow

Faculty, Staff and Students Publications

BACKGROUND: Advances in our understanding of the molecular biology of meningiomas have led to significant gains in the ability to predict patient prognosis and tumor recurrence and to identify novel targets for therapeutic design. Specifically, classification of meningiomas based on DNA methylation has greatly improved our ability to risk stratify patients, however new questions have arisen in terms of the underlying impact these DNA-methylation signatures have on meningioma biology.

METHODS: This study utilizes RNA-sequencing data from 486 meningioma samples corresponding to 3 meningioma DNA-methylation groups (merlin-intact, immune-enriched, and hypermitotic), followed by in vitro experiments utilizing human meningioma cell lines.

RESULTS: …

Immune Landscape Of Isocitrate Dehydrogenase-Stratified Primary And Recurrent Human Gliomas, Pravesh Gupta, Minghao Dang, Shivangi Oberai, Simona Migliozzi, Rakesh Trivedi, Gayatri Kumar, Mekenzie Peshoff, Nancy Milam, Aml Ahmed, Krishna Bojja, Tuan M Tran, Joy Gumin, Carlos Kamiya-Matsuoka, Jason Huse, Kathryn Cox, Jianzhuo Li, Huma Shehwana, Sameer A Sheth, Rodriguez Saxon, Sun Baohua, Brittany Parker Kerrigan, Atul Maheshwari, Edwin Roger Parra Cuentas, Nicholas E Navin, Amy B Heimberger, Frederick F Lang, Antonio Iavarone, Karen Clise-Dwyer, Linghua Wang, Krishna P Bhat

Faculty, Staff and Student Publications

Background: Human gliomas are classified using isocitrate dehydrogenase (IDH) status as a prognosticator; however, the influence of genetic differences and treatment effects on ensuing immunity remains unclear.

Methods: In this study, we used sequential single-cell transcriptomics on 144 678 and spectral cytometry on over 2 million immune cells encompassing 48 human gliomas to decipher their immune landscape.

Results: We identified 22 distinct immune cell types that contribute to glioma immunity. Specifically, brain-resident microglia (MG) were reduced with a concomitant increase in CD8+ T lymphocytes during glioma recurrence independent of IDH status. In contrast, IDH-wild type-associated patterns, such as an abundance …