Digital Commons Network^™

Expired Dexon Drug As Green Inhibitor For Carbon Steel In Simulated Seawater Medium, Shams Naji, Rana Anaee, Sadeer Majeed

Journal of Applied Sciences and Nanotechnology

The addition of expired drugs as green inhibitors takes high attention as an alternative material compared with other expensive, toxic, non-available chemical inhibitors. In this study, the Dexon drug was suggested as a green inhibitor for commonly used carbon steel in (3.5% NaCl solution) within a temperature range of 303 to 333 K. Corrosion/inhibition investigation was done by potentiostatic analysis to record the corrosion potential, corrosion current density, and Tafel slopes for the added four concentrations (4, 20, 40 and 60 mL/L) of the drug. The polarization resistances and inhibition efficiencies reached the highest value of (25.86×10-3 Ω.cm2) and (95.57%) …

Studies Of Media To Maximize The Expression Of Bacterial Metalloprotease Genes As Potential Therapeutics For Human Alzheimer’S Disease, Uday Kiran Chigurupati

Honors Capstones

This study investigates the potential of bacterial metalloprotease genes as therapeutic agents for Alzheimer's Disease (AD). The research focuses on Pseudomonas 20ei1 (P. 20ei1), a bacterial species isolated from lagoon water, which contains pitrilysin genes that share similarities with human insulysin. Six pqqL-related genes were identified in P. 20ei1 for this study. The research methodology involved bioinformatic studies, bacterial cell culture in various media, RNA extraction, purification, and cDNA synthesis. Quantitative PCR analysis was performed to analyze gene expression levels in different cDNA samples. The bacteria grown in the medium with insoluble phosphate has shown higher expression of selected genes …

Gardner-Webb Incoming First-Year Students Form Strong Bonds In Living-Learning Communities (Llc), Office Of University Communications

Gardner-Webb NewsCenter Archive

After successfully introducing Gardner-Webb University’s first living-learning community (LLC) last year, the Division of Student Success in collaboration with the Office of Housing and Residence Education added two more LLCs for the 2024 Fall Semester. Incoming first-year students could choose to participate in one of three LLCs: Faith, STEM or Honors.

Teacher’S Perspectives Regarding Kindergarten Students’ Behaviors And Social Skills Post-Pandemic, Latalia Middlebrook Robbins

Walden Dissertations and Doctoral Studies

Kindergarten teachers and support professionals in the social-emotional community within a local school district experienced changes in student behaviors and social skills since returning to in-person schooling following the pandemic. After COVID-19, teachers found themselves engaging students who presented a lack of social skill development, contributing to the behaviors within the classroom microsystem. This study used Urie Bronfenbrenner’s ecological systems theory to support research efforts to examine how learning environments and interactions contributed to students’ preparation for Kindergarten. The research explored 12 Kindergarten teachers' and support professionals' perspectives concerning Kindergarten student behaviors and social skills. The research design was an …

Regulatory Dynamics Of Arginine Metabolism In Staphylococcus Aureus, Itidal Reslane, Gabrielle F. Watson, Luke D. Handke, Paul D. Fey

Open Access Articles Funded by the University of Nebraska Open Access Publishing Agreements or the former UNO Open Access Fund

Staphylococcus aureus is a highly significant pathogen with several well studied and defined virulence factors. However, the metabolic pathways that are required to facilitate infection are not well described. Previous data have documented that S. aureus requires glucose catabolism during initial stages of infection. Therefore, certain nutrients whose biosynthetic pathway is under carbon catabolite repression and CcpA, including arginine, must be acquired from the host. However, even though S. aureus encodes pathways to synthesize arginine, biosynthesis of arginine is repressed even in the absence of glucose. Why is S. aureus a functional arginine auxotroph? This review discusses recently described regulatory …

Lipid Discovery Enabled By Sequence Statistics And Machine Learning, Priya M. Christensen, Jonathan Martin, Aparna Uppuluri, Luke R. Joyce, Yahan Wei, Ziqiang Guan, Faruck Morcos, Kelli L. Palmer

School of Podiatric Medicine Publications

Bacterial membranes are complex and dynamic, arising from an array of evolutionary pressures. One enzyme that alters membrane compositions through covalent lipid modification is MprF. We recently identified that Streptococcus agalactiae MprF synthesizes lysyl-phosphatidylglycerol (Lys-PG) from anionic PG, and a novel cationic lipid, lysyl-glucosyldiacylglycerol (Lys-Glc-DAG), from neutral glycolipid Glc-DAG. This unexpected result prompted us to investigate whether Lys-Glc-DAG occurs in other MprF-containing bacteria, and whether other novel MprF products exist. Here, we studied protein sequence features determining MprF substrate specificity. First, pairwise analyses identified several streptococcal MprFs synthesizing LysGlc-DAG. Second, a restricted Boltzmann machine-guided approach led us to discover an …

Mapping Out, Hector Escobar, Erik Ziedses Des Plantes

University Libraries Blog Archive

On Nov. 20, Roesch Library hosted its first GIS Day, a global event promoting the use and understanding of geographic information systems.

Evaluation Of The Efficacy Of Transfer Energy Capacitive And Resistive Therapy In Patients With Knee Osteoarthritis, Özge Tezen, Emi̇ne Esra Bi̇li̇r, Öznur Uzun, Duygu Yaniktaş, Başak Şentürk, Evren Yaşar

Turkish Journal of Medical Sciences

Background/aim: The aim of present study was to investigate the treatment efficacy of transfer energy capacitive and resistive (TECAR) therapy, a modern application that has been increasingly used in recent years, compared to conventional physical therapy (CPT) applications in the treatment of knee osteoarthritis, according to the evaluation of clinical findings of patients.Materials and methods: A total of 54 patients, aged between 40 and 75 years, were randomly divided into two groups. Both groups received CPT. Group 2 received TECAR therapy in addition to CPT, applied three times a week for two weeks, for a total of 6 sessions. All …

Achillea Millefolium Ameliorates Doxorubicin-Induced Renal Injury Via Inhibition Of Oxidative Stress And Inflammation In Rats, Mohammed Shaiea, Yi̇mi̇ng Dong, Saleh Alomisy, Hassan Al-Mahbashi, Guozhong Zhang, Chuan Wang

Turkish Journal of Medical Sciences

Background/aim: Doxorubicin (Dox) is a potent anticancer medication. Nevertheless, due to nephrotoxicity, its clinical application is restricted. Achillea millefolium (AM) is a traditional medicine used against several disturbances, including kidney disorders. Therefore, this work aims to investigate the preventative properties of AM extract (AME) and their mechanisms against nephrotoxicity caused by Dox in rats. Materials and methods: The rats were assigned randomly to six groups, including a control group, Dox group (5 mg/kg/week via i.p. for 4 weeks), two groups, AME (100 or 200 mg/kg, orally for 28 days), and the last two groups, Dox+ AME (100 or 200 mg/kg, …

Molecular Docking Simulation Of Bioactive Compounds From Curcuma Longa Against Helicobacter Pylori, Alex J. Mbise, Pulapa V. Kanaka Rao, F.M. Stanley

Tanzania Journal of Science

Peptic ulcers are open sores that form on the lining of the stomach and upper small intestine and the primary causative pathogen is Helicobacter pylori. In this research, the inhibitory activities of bioactive compounds from Curcuma longa, against H. pylori (8H52) were studied. Eighty-three significant bioactive compounds were selected from the Zinc database and were initially screened using absorption, distribution, metabolism, excretion, and toxicity properties based on compliance with the Pfizer rule. Molecular docking studies were carried out with specific docking site dimensions of values x: -37.3658, y: -4.1028, and z: -22.8563, to identify the binding affinities and interactions. Ten …

African Women Custodians: Teaching, Toponymy And Tacit Reverie In Heritage Education, Tokie Laotan-Brown

Wagadu: A Journal of Transnational Women's & Gender Studies

This paper investigates the critical role of women in African cultures as guardians of cultural heritage. It explores their central function in transmitting traditional ecological knowledge and practices across generations through established pedagogical methods, toponymic knowledge systems (place-names within cultural landscapes), and the cultivation of tacit knowledge. The paper emphasizes the significance of women's contributions to environmental education, often overlooked or undervalued within Western scientific paradigms. This marginalization restricts access to resources and participation in decision-making processes. It argues for the integration of traditional knowledge systems, informed by women's expertise, into formal education and pedagogy. This inclusion serves to validate …

Increased Thermal Stability And Retained Antibacterial Properties In A Sulbactam And Amantadine Salt: Towards Effective Antibacterial-Antiviral Combination Therapies, Josephine Bicknell, Ivan Bondarenko, Alice Colatrella, Elani J. Cabrera-Vega, Jesus Daniel Loya, Delbert S. Botes, Jay L. Mellies, Gonzalo Campillo-Alvarado

Chemistry Faculty Publications and Presentations

A clinically relevant multidrug solid comprising sulbactam (β-lactamase inhibitor) and amantadine (antiviral) displays enhanced thermal stability and sustained antibacterial activity compared to starting materials.

We describe the formation of a multidrug salt comprising sulbactam (SUL, β-lactamase inhibitor) and amantadine (AMNH, antiviral). Physicochemical investigation of the SUL·AMNH salt revealed enhanced thermal stability compared to pristine starting materials. In vitro studies found that salt formation in SUL·AMNH does not disrupt antibacterial activity against model organisms Escherichia coli and Staphylococcus epidermidis. To our knowledge, we show the first β-lactamase inhibitor-antiviral salt where both components have been approved by the U.S. Food and Drug …

International Methods Of Offender Profile Construction, Standardization, And Effective Criminal Investigative Processes, Savannah Peoples

Walden Dissertations and Doctoral Studies

Offender profiling is a tool used within criminal investigations to infer offender characteristics according to evidence collected from a crime scene. The practice of offender profiling has been applied since the Roman era, empirically developed, and recently popularized in media depictions. Existing studies demonstrate that there is a lack of peer reviewed literature that examines current methods of criminal profile construction, opportunities for standardization, and effective criminal investigative processes. Motivation for this study came after reviewing research that revealed high rates of serious and violent international serial offending, noted as a major challenge for law enforcement agencies, and a significant …

The Effect Of Applied Mental Health Courses On College Student Anxiety And Perfectionism, Nathan Thomas Rhees

Theses and Dissertations

University students face a variety of mental health challenges, including increasingly high rates of anxiety. As demand for college counseling services among student populations rises, universities have an increasing need for effective and resource-efficient methods of intervention that support student mental health on their campuses. One area in need of research is that of using university classes themselves as a form of preventive intervention. If effective, this kind of preventive measure might be a particularly attractive method of reducing incidence of mental illness on campus and decreasing demand on university therapy resources. This study involves students at a private university …

Ai-Driven Anomaly Detection In Cybersecurity, Mohamed Almansoori

Theses

India has witnessed a huge level of digitization in the last two decades which has greatly impacted its economy and society. But the increasing use of technology has also intensified the number and level of threats to cyberspace in the country. Signature-based detection systems that have dominated the cybersecurity field for years have not been up to the challenge of modern attackers who use more complex and diverse attack methods. These challenges are well understood and this research focuses on improving the cybersecurity anomaly detection in India during the period between 2003 and 2022 using advanced AI and machine learning. …

Angiogenin-Catalyzed Cleavage Within Trna Anticodon-Loops Identified By Cp-Rna-Seq, Megumi Shigematsu, Ryuma Matsubara, Justin Gumas, Takuya Kawamura, Yohei Kirino

Computational Medicine Center Faculty Papers

Angiogenin (Ang), an endoribonuclease belonging to the RNase A superfamily, cleaves the anticodon-loops of tRNAs to produce tRNA-half molecules. Although previous studies have demonstrated the involvement of Ang in the pathobiology of neurodegenerative disorders, the characterization of Ang-generated tRNA halves in neuronal cells remains limited. This is partly due to the technical limitations of standard RNA-seq methods, which cannot capture Ang-generated RNAs containing a 2',3'-cyclic phosphate (cP). In this report, we established an Ang-treatment model using SH-SY5Y, a human neuroblastoma cell line, and demonstrated Ang-dependent accumulation of tRNA halves. By performing cP-RNA-seq, which selectively captures cP-containing RNAs, we identified Ang-generated …

The Long Shadow Of Infection: The Long-Term Implications Of Herpes Simplex Virus And M. Tuberculosis Infection, Lydia Howell

Theses and Dissertations

Infectious diseases and autoimmune diseases, such as multiple sclerosis (MS) and type 1 diabetes (T1D), have traditionally been seen as distinct, with separate etiologies and courses of disease. Recent research has shown that infection and autoimmune disease are more closely woven together than previously thought; certain pathogens like Epstein-Barr virus and M. tuberculosis have been shown to cause long-term disruption to the immune system, for example (Bordignon et al 2011, #1) (Zhang et al 2023, #2) (Bjornevik et al 2023, #3). Conversely, a distinct pattern has emerged in developed countries where as rates of infection fall, rates of autoimmune disease …

Induction Of Tier 2 Hiv-Neutralizing Iga Antibodies In Rhesus Macaques Vaccinated With Bg505.664 Sosip, Justin C. Smith, Prabhu S. Arunachalam, Traci H. Legere, Lisa A. Cavacini, Eric Hunter, Bali Pulendran, Rama R. Amara, Pamela A. Kozlowski

School of Graduate Studies Faculty Publications

BACKGROUND: A goal of mucosal human immunodeficiency virus type 1 (HIV-1) vaccines is to generate mucosal plasma cells producing polymeric IgA (pIgA)-neutralizing antibodies at sites of viral entry. However, vaccine immunogens capable of eliciting IgA neutralizing antibodies (nAbs) that recognize tier 2 viral isolates have not yet been identified. METHODS: To determine if stabilized native-like HIV-1 envelope (Env) trimers could generate IgA nAbs, we purified total IgA and IgG from the banked sera of six rhesus macaques that had been found in a previous study to develop serum nAbs after subcutaneous immunization with BG505.664 SOSIP and 3M-052 adjuvant, which is …

International Methods Of Offender Profile Construction, Standardization, And Effective Criminal Investigative Processes, Savannah Peoples

Walden Dissertations and Doctoral Studies

Offender profiling is a tool used within criminal investigations to infer offender characteristics according to evidence collected from a crime scene. The practice of offender profiling has been applied since the Roman era, empirically developed, and recently popularized in media depictions. Existing studies demonstrate that there is a lack of peer reviewed literature that examines current methods of criminal profile construction, opportunities for standardization, and effective criminal investigative processes. Motivation for this study came after reviewing research that revealed high rates of serious and violent international serial offending, noted as a major challenge for law enforcement agencies, and a significant …

Examining The Relationship Between Personality, Values, And Treatment Retention In Substance Abuse Programs: A Quantitative Correlation Analysis, Jonathan Ciampi

Walden Dissertations and Doctoral Studies

Substance Use Disorder (SUD) continues to be a global issue with devasting effects on the community, loved ones, and individuals suffering from SUD. Healthcare professionals continue to develop improved treatments for addressing patient needs, yet treatment abandonment remains high. While studies have examined treatment retention factors, few have examined the relationship between personality traits, values, and treatment retention. This study examined the relationship between the five-factor model of personality traits, the theory of formal axiology of values, and the days in treatment for adults in a telehealth substance abuse outpatient clinic in the United States. The study assessed the combined …

Characterizing Full-Time, Non-Tenure-Track Teaching Faculty In Biology, Desiree Forsythe, Jeremy L. Hsu

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Institutions of higher education play a major role in teaching undergraduate students. Historically, most courses have been taught by tenure-track (TT) faculty who may also be responsible for research or scholarly activities. However, a recent shift from “teaching-intensive” TT faculty to “teaching-only” contingent faculty off the tenure track has highlighted the importance of understanding the experiences of contingent faculty. While there have been an increasing number of studies examining the experiences of part-time contingent faculty, few studies have directly surveyed the increasing number of full-time, non-tenure-track (NTT) teaching faculty in science, technology, engineering, and mathematics (STEM) to capture their experiences, …

Teacher’S Perspectives Regarding Kindergarten Students’ Behaviors And Social Skills Post-Pandemic, Latalia Middlebrook Robbins

Walden Dissertations and Doctoral Studies

Kindergarten teachers and support professionals in the social-emotional community within a local school district experienced changes in student behaviors and social skills since returning to in-person schooling following the pandemic. After COVID-19, teachers found themselves engaging students who presented a lack of social skill development, contributing to the behaviors within the classroom microsystem. This study used Urie Bronfenbrenner’s ecological systems theory to support research efforts to examine how learning environments and interactions contributed to students’ preparation for Kindergarten. The research explored 12 Kindergarten teachers' and support professionals' perspectives concerning Kindergarten student behaviors and social skills. The research design was an …

Examining The Relationship Between Personality, Values, And Treatment Retention In Substance Abuse Programs: A Quantitative Correlation Analysis, Jonathan Ciampi

Walden Dissertations and Doctoral Studies

Substance Use Disorder (SUD) continues to be a global issue with devasting effects on the community, loved ones, and individuals suffering from SUD. Healthcare professionals continue to develop improved treatments for addressing patient needs, yet treatment abandonment remains high. While studies have examined treatment retention factors, few have examined the relationship between personality traits, values, and treatment retention. This study examined the relationship between the five-factor model of personality traits, the theory of formal axiology of values, and the days in treatment for adults in a telehealth substance abuse outpatient clinic in the United States. The study assessed the combined …

Exploration In Cell Growth: Rac3 And Its Effect On Ewing Sarcomas Tumorigenic Properties, Allie Woods, Samuel De La Cruz, Catherine Garcia, Terry Jo Shackleford

Cell and Molecular Methods

Multiple experiments were conducted to measure the effectiveness of certain genes on cell growth. In the exploration of this process, RAC3 acted as the target gene and was applied to both SOX18 overexpressing and normal expressing cells (control). RAC3 is a gene that typically controls the intercellular signaling pathway of a cell. This means it helps with the movement of cells and allows for migration.The purpose of these experiments were to determine how the gene RAC3 would work when introduced to carcinogenic cells, specifically Ewing Sarcoma. Typically, RAC3 acts as a messenger for the cell, but when in conjunction with …

The Effect Of Knockdown Of Gene Kit On Sox18 Gene Expression Through The Jak2/Stat3 Signaling Pathway Within An Ewing Sarcoma Cell, Samantha Aragon, Cristina Herrera, Felicity Rodriguez, Riley Smith, Terry Jo Shackleford

Cell and Molecular Methods

Ewing sarcoma is an aggressive cancer that affects children & adolescents. Common treatments include surgery, radiation, and chemotherapy, yet individuals have a poor survival rate and often develop side effects from treatment, including fertility issues, secondary cancer, heart problems, and growth abnormalities.1SOX18 is a gene identified to aid in cell proliferation and cell differentiation, leading to the development of several cancers, such as gastric, bladder, and lung cancer.2,3 SOX18 resides in the JAK2/STAT3 pathway as a regulator, along with another gene named KIT that instigates the formation of the cell signaling pathway.4KIT is a transmembrane tyrosine kinase, which instigates cell …

The Effects Of Ewing Sarcoma Through The Overexpression And Knockdown Of Transcription Factors Sox18 And Stat1, Victoria Castillo, Melany Cervantes, Nicole Robles, Terry Jo Shackleford

Cell and Molecular Methods

SOX18, a transcription factor with a DNA-binding HMG domain, plays a critical role in regulatory processes linked to cancer progression. Exhibiting oncogenic properties, SOX18 has been linked to various cancers, where it promotes tumor growth by enhancing cell invasion, uncontrolled proliferation, and resistance to apoptosis through dysregulated signaling pathways. STAT1, a protein crucial for immune system regulation, is prominently expressed in immune-associated tissues like the lymph nodes, and bone marrow, suggesting it could play a role in tumor growth. Ewing Sarcoma (EWS), an aggressive cancer that targets bone and soft tissue, carries a high mortality rate of 90% without treatment …

Assessment Of Bite Force And Masseter And Temporal Muscles Thickness In Adult Women Undergoing Buccal Fat Pad Removal Surgery–Bichectomy, Thamyres Branco, Marcelo Palinkas, Alice Helena De Lima Santos Cardoso, Nicole Barbosa Bettiol, Paulo Batista De Vasconcelos, Gabriella Simi Gariba Silva, Jéssica Brandani Chiaratto, Patricia Santos De Lima, Samuel Porfírio Xavier, Isabela Hallak Regalo, Selma Siéssere, Simone Cecilio Hallak Regalo

Journal of Dentistry Indonesia

Objective: Bichectomy, a surgical procedure that removes the buccal fat pad, can affect masticatory function, including bite force and muscle thickness, in adult women. This study aimed to assess the bite force as well as masseter and temporal muscle thicknesses before and 30 and 60 days after bichectomy in adult women. Methods: This longitudinal study included a sample of 20 adult women (mean age 31.1 ± 9.84 years), with normal occlusion and no history of temporomandibular disorder. The molar bite force was assessed using a dynamometer, while the masseter and temporal muscle thicknesses at rest and during dental …

Characterization Of Fresh Bovine Amnion Membrane Combined With Hydroxyapatite As Candidate Scaffold For Alveolar Bone Tissue Engineering, Octarina Octarina, Elly Munadziroh

Journal of Dentistry Indonesia

Objective: This research aims to analyze the characteristics of a BAM-HA biocomposite with ratios of 4:1 and 4:2. Methods: This research is an in vitro laboratory experiment that starts by grinding fresh BAM to produce amnion slurry and then, adding HA powder to the slurry and carrying out freeze-drying on the slurry. Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscope-Energy dispersive X-ray (SEM-EDX) tests are used to analyze the characteristics of the BAM-HA biocomposite. Results: The FTIR test results showed that the BAM-HA biocomposite had amide functional groups I, II, III, A, B, OH, CO_{3 …}

Histomorphometric Assessment Of Vibrational Forces On The Extended Midpalatal Suture, Aylin Pasaoglu Bozkurt, Özge Özdal Zincir, Asli Emincik

Journal of Dentistry Indonesia

Objective: This study determined the effects of vibrational forces on bone formation in the retention period of an expanded mid-palatal, histomorphometric suture in a rat model. Methods: Twenty-four 7–8- to 8-week-old male Wistar rats weighing 190.25 ± 15.35 g were separated into groups: only expansion (G1), expansion and 30 Hz vibration application (G2), and expansion and 111 Hz vibration application (G3). After 5 days of expansion, there were 12 days of retention. All animals were sacrificed, and their premaxilla were dissected. A histological examination evaluated the number of osteoblasts, osteoclasts, and capillaries, the amount of mineralized area, and …

Knowledge, Attitude, Oral Hygiene Practice And Self-Perception On Periodontal Health Among Older Adult Population In Malaysia, Annabel Shanta Edwin Thomas, Haslina Taib, Zurairah Berahim, Siti Lailatul Akmar Zainuddin, Munirah Mohd Adnan

Journal of Dentistry Indonesia

Objective: This study aimed to assess the knowledge, attitude, oral hygiene practice, and self-perception of periodontal health among the older adult population in Malaysia and its association with demographic factors. Methods: An online questionnaire assessing their knowledge, attitude, oral hygiene practices, and self-perception of periodontal disease was distributed to adults aged 50 years and above. Results: 397 respondents participated. The mean knowledge score was 1.36 (SD 0.92), significantly associated with gender, ethnicity, education, and occupation. About 63% of respondents know that poor oral hygiene is related to general health and is significantly associated with ethnicity (p = …