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Washington University Magazine, December 2024

Washington University Magazine

No abstract provided.

Tract-Based Analyses Of White Matter In Schizophrenia, Bipolar Disorder, Aging, And Dementia Using High Spatial And Directional Resolution Diffusion Imaging: A Pilot Study, Daniel Mamah, Shingshiun Chen, Joshua S Shimony, Michael P Harms

2020-Current year OA Pubs

INTRODUCTION: Structural brain connectivity abnormalities have been associated with several psychiatric disorders. Schizophrenia (SCZ) is a chronic disabling disorder associated with accelerated aging and increased risk of dementia, though brain findings in the disorder have rarely been directly compared to those that occur with aging.

METHODS: We used an automated approach to reconstruct key white matter tracts and assessed tract integrity in five participant groups. We acquired one-hour-long high-directional diffusion MRI data from young control (CON, n =28), bipolar disorder (BPD, n =21), and SCZ (n =22) participants aged 18-30, and healthy elderly (ELD, n =15) and dementia (DEM, n …

Characterization Of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels In Oligodendrocytes., Kyle A Lyman, Ye Han, Andrew P Robinson, Samuel E Weinberg, Daniel W Fisher, Robert J Heuermann, Reagan E Lyman, Dong Kyu Kim, Andreas Ludwig, Navdeep S Chandel, Mark D Does, Stephen D Miller, Dane M Chetkovich

2020-Current year OA Pubs

Mature oligodendrocytes (OLG) are the myelin-forming cells of the central nervous system. Recent work has shown a dynamic role for these cells in the plasticity of neural circuits, leading to a renewed interest in voltage-sensitive currents in OLG. Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and their respective current (I

Targeting Of Oncogenic Aaa-Atpase Trip13 Reduces Progression Of Pancreatic Ductal Adenocarcinoma, Farrukh Afaq, Sumit Agarwal, Prachi Bajpai, Sameer Al Diffalha, Hyung-Gyoon Kim, Shajan Peter, Moh'd Khushman, Subhash C Chauhan, Priyabrata Mukherjee, Sooryanarayana Varambally, Upender Manne

2020-Current year OA Pubs

Thyroid hormone receptor-interacting protein 13 (TRIP13) is involved in cancer progression, but its role in pancreatic ductal adenocarcinoma (PDAC) is unknown. Thus, we assessed the expression, functional role, and mechanism of action of TRIP13 in PDAC. We further examined the efficacy of TRIP13 inhibitor, DCZ0415, alone or in combination with gemcitabine on malignant phenotypes, tumor progression, and immune response. We found that TRIP13 was overexpressed in human PDACs relative to corresponding normal pancreatic tissues. TRIP13 knockdown or treatment of PDAC cells with DCZ0415 reduced proliferation and colony formation, and induced G2/M cell cycle arrest and apoptosis. Additionally, TRIP13 knockdown or …

Leveraging Murine Models Of The Neurofibromatosis Type 1 Cancer Predisposition Syndrome To Elucidate The Cellular Circuits That Drive Pediatric Low-Grade Glioma Formation And Progression, David H Gutmann

2020-Current year OA Pubs

Brain tumors are the leading cause of cancer-related death in children, where low-grade gliomas (LGGs) predominate. One common hereditary cause for LGGs involves neurofibromatosis-1 (NF1) gene mutation, as seen in individuals with the NF1 cancer predisposition syndrome. As such, children with NF1 are at increased risk of developing LGGs of the optic pathway, brainstem, cerebellum, and midline brain structures. Using genetically engineered mouse models, studies have revealed both cell-intrinsic (MEK signaling) and stromal dependencies that underlie their formation and growth. Importantly, these dependencies represent vulnerabilities against which targeted agents can be used for preclinical investigation prior to clinical translation.

Neurosteroids Mediate And Modulate The Effects Of Pro-Inflammatory Stimulation And Toll-Like Receptors On Hippocampal Plasticity And Learning, Yukitoshi Izumi, Kazuko A. O'Dell, Anil G. Cashikar, Steven M. Paul, Douglas F. Covey, Steven J. Mennerick, Charles F. Zorumski

2020-Current year OA Pubs

Pro-inflammatory changes contribute to multiple neuropsychiatric illnesses. Understanding how these changes are involved in illnesses and identifying strategies to alter inflammatory responses offer paths to potentially novel treatments. We previously found that acute pro-inflammatory stimulation with high (μg/ml) lipopolysaccharide (LPS) for 10-15 min dampens long-term potentiation (LTP) in the hippocampus and impairs learning. Effects of LPS involved non-canonical inflammasome signaling but were independent of toll-like receptor 4 (TLR4), a known LPS receptor. Low (ng/ml) LPS also inhibits LTP when administered for 2-4 h, and here we report that this LPS exposure requires TLR4. We also found that effects of low …

Control Of Acute Myeloid Leukemia And Generation Of Immune Memory In Vivo Using Amv564, A Bivalent Bispecific Cd33 X Cd3 T Cell Engager, Linda G. Eissenberg, Julie K. Ritchey, Michael P. Rettig, Dilan A. Patel, Kiran Vij, Feng Gao, Victoria Smith, Tae H Han, John F. Dipersio

2020-Current year OA Pubs

Off-the-shelf immunotherapeutics that suppress tumor growth and provide durable protection against relapse could enhance cancer treatment. We report preclinical studies on a CD33 x CD3 bivalent bispecific diabody, AMV564, that not only suppresses tumor growth, but also facilitates memory responses in a mouse model of acute myelogenous leukemia (AML). Mechanistically, a single 5-day treatment with AMV564 seems to reduce tumor burden by redirection of T cells, providing a time window for allogeneic or other T cells that innately recognize tumor antigens to become activated and proliferate. When the concentration of bispecific becomes negligible, the effector: target ratio has also shifted, …

Cardiovascular Disease In Firefighters, Emily Jordan Baldwin

Doctor of Nursing Practice Projects

This quality improvement project aimed to address the problem of increased cardiovascular events in firefighters in a county in the foothills of North Carolina. Nola Pender and Dorothy Orem’s theories were utilized for this project. The literature review exhibits the prevalence and significance of cardiovascular disease in firefighters. The literature review demonstrates the benefits healthy eating and exercise have on the heart and towards the prevention of cardiovascular disease. A pre- and post-test was utilized for this project and allowed for the collection of data appropriately. The results showed education on cardiovascular disease, exercise, and healthy eating increased the knowledge …

Increased Concern Of Suicide Behaviors In The Adolescent Population, Jaime Shareen-Porter Earle

Doctor of Nursing Practice Projects

This quality improvement project focused on addressing the concern for the increase of depression, anxiety, and self-harm behaviors among adolescents, which can lead to suicidal ideation and attempts. Suicide behaviors have increased among adolescents, and research revealed that suicide is the second leading cause of death in this population. An educational PowerPoint was presented to members of the community on suicide behaviors, how to identify the behaviors, and where to get support and resources in the community. Pre- and post-surveys were used to assess the participants' knowledge and confidence levels before and after project implementation. The surveys contained the same …

Mental Health And The Black Church: Decreasing The Division In Divinity, Latonya Gray

Doctor of Nursing Practice Projects

Racial disparities in mental health care persist for Black Americans, with only one in three who need treatment receiving it. The care they do receive is often of a poorer quality than that of other groups. Faith communities serving Black members could help address this gap by partnering with mental health professionals to meet congregants' needs. With mental health now at the forefront of public discourse, considering the specific needs of the Black community is critical. Black Americans face disproportionate mental health burdens but are more likely to seek counsel from clergy due to cultural stigma. Educating clergy on mental …

Community Educational Session On Identifying The Signs Of Depression And Resources Available, Desiree Young

Doctor of Nursing Practice Projects

The DNP Project was designed to educate the community on identifying the signs of depression and the community resources available to them. The intent of the project was to improve the community’s knowledge of depression symptoms and the community resources available that offer assistance to those dealing with these symptoms. There is a great need for education on depression signs, as many in various communities suffer from untreated depression, which often leads to negative outcomes. The impact of the project implementation was measured by using pre- and post-survey data evaluating the participants' overall knowledge of the signs of depression, stigma …

Benefits Of Patient-Centered Education For Type 2 Diabetes Mellitus, Paige Bortnick

Doctor of Nursing Practice Projects

Type 2 diabetes is a chronic, lifelong illness that affects millions of individuals in the United States. The rate of diabetes has doubled over the last 20 years in America and has climbed to be the seventh leading cause of death amongst Americans. The purpose of this project was to determine if participants in the target community demonstrated increased knowledge and awareness of diabetes after receiving education. A pre-intervention and post-intervention survey was given to determine if the education was effective. Data collected revealed an increased awareness and knowledge regarding the prevalence of type 2 diabetes, lifestyle, and diet modification.

Community Education: Impact On Earlier Recognition Of Stroke, Lauren Kahn

Doctor of Nursing Practice Projects

Cerebrovascular disease was identified as the fourth leading cause of death in Cleveland County, North Carolina from 2014 to 2018 (Cleveland County Public Health Center, 2020). Community education was identified as a potential solution to decreasing the number of community members affected by cerebrovascular disease and resulting death and disability. Five community classes were taught with 97 participants. Pre-, post-, and 1-week post-class surveys were distributed to assess baseline knowledge, knowledge gain, and knowledge retention, with 74 pre-class, 72 post-class, and 28 1-week post-class surveys returned. Of these surveys, after exclusionary criteria were used, 40 pre-class, 53 post-class, and 22 …

Cathepsin C Role In Inflammatory Gastroenterological, Renal, Rheumatic, And Pulmonary Disorders, Ali A Aghdassi, Christine Pham, Lukas Zierke, Vincent Mariaule, Brice Korkmaz, Moez Rhimi

2020-Current year OA Pubs

Cathepsin C (CatC, syn. Dipeptidyl peptidase I) is a lysosomal cysteine proteinase expressed in several tissues including inflammatory cells. This enzyme is important for maintaining multiple cellular functions and for processing immune cell-derived proteases. While mutations in the CatC gene were reported in Papillon-Lefèvre syndrome, a rare autosomal recessive disorder featuring hyperkeratosis and periodontitis, evidence from clinical and preclinical studies points toward pro-inflammatory effects of CatC in various disease processes that are mainly mediated by the activation of neutrophil serine proteinases. Moreover, tumor-promoting effects were ascribed to CatC. The aim of this review is to highlight current knowledge of the …

Cep120 Is Essential For Kidney Stromal Progenitor Cell Growth And Differentiation, Ewa Langner, Tao Cheng, Eirini Kefaloyianni, Charles Gluck, Baolin Wang, Moe R Mahjoub

2020-Current year OA Pubs

Mutations in genes that disrupt centrosome structure or function can cause congenital kidney developmental defects and lead to fibrocystic pathologies. Yet, it is unclear how defective centrosome biogenesis impacts renal progenitor cell physiology. Here, we examined the consequences of impaired centrosome duplication on kidney stromal progenitor cell growth, differentiation, and fate. Conditional deletion of the ciliopathy gene Cep120, which is essential for centrosome duplication, in the stromal mesenchyme resulted in reduced abundance of interstitial lineages including pericytes, fibroblasts and mesangial cells. These phenotypes were caused by a combination of delayed mitosis, activation of the mitotic surveillance pathway leading to apoptosis, …

Non-Invasive Auricular Vagus Nerve Stimulation For Subarachnoid Hemorrhage (Navsah): Protocol For A Prospective, Triple-Blinded, Randomized Controlled Trial, Anna Huguenard, Gansheng Tan, Gabrielle Johnson, Markus Adamek, Andrew Coxon, Terrance Kummer, Joshua Osbun, Ananth Vellimana, David Limbrick, Gregory Zipfel, Peter Brunner, Eric Leuthardt

2020-Current year OA Pubs

BACKGROUND: Inflammation has been implicated in driving the morbidity associated with subarachnoid hemorrhage (SAH). Despite understanding the important role of inflammation in morbidity following SAH, there is no current effective way to modulate this deleterious response. There is a critical need for a novel approach to immunomodulation that can be safely, rapidly, and effectively deployed in SAH patients. Vagus nerve stimulation (VNS) provides a non-pharmacologic approach to immunomodulation, with prior studies demonstrating VNS can reduce systemic inflammatory markers, and VNS has had early success treating inflammatory conditions such as arthritis, sepsis, and inflammatory bowel diseases. The aim of the Non-invasive …

Early-Career Georgia Agriculture Teachers' Agricultural Mechanics Professional Development Needs, Christopher Crump

Murray State Theses and Dissertations

Agricultural mechanics is a prominent topic in Georgia Agricultural Education, as it is the agricultural subject matter area with the second-largest enrollment across all school systems in the state. However, with Georgia agricultural teacher preparation programs only requiring three credit hours in the subject matter area, questions arise as to whether Georgia agriculture teachers are properly prepared to teach this subject matter area, especially if those teachers did not complete a traditional teacher preparation program. This nonexperimental, quantitative study assessed early-career Georgia agriculture teachers’ professional developmental (PD) needs in agricultural mechanics. Qualtrics was utilized to collect responses to a research …

Changing Demographics In Career & Technical Student Organizations, Elizabeth Hunt Galloway

Murray State Theses and Dissertations

The state of Georgia’s demographics has consistently changed in the last twenty years. Since 2010, the population of Georgia has steadily increased by 1% (Georgia Population by Year, County, Race, & More, 2023). A mirror of the state population increases occurred in the school system with 1% growth each year between 2010 and 2022 (Georgia Population by Year, County, Race, & More, 2023). The demographics of the state of Georgia has changed along with the population with an increase in Hispanic and Black population while the White population has decreased (Hauer, n.d.). The need to increase membership in Career Technical …

Administrator Perceptions Of Factors Distinguishing School-Based Agricultural Education Programs As Successful, Mckinley Hunter

Murray State Theses and Dissertations

Agricultural education is a vital component of the Career, Technical, and Agricultural Education (CTAE) framework that encompasses classroom instruction, supervised agricultural experience (SAE), and involvement in the National FFA Organization. This study gathered Georgia high school administrators’ perceptions of factors distinguishing school-based agricultural education (SBAE) programs as successful. Administrators identified various components important to program success for each are of the agricultural three-component model. Administrators in this study, prioritized involvement in the National FFA Organization over the other constructs, indicating that the traditional view of the three-component model can be dependent upon local needs and priorities. This research can be …

Listen Up: Perceptions Of Audiobooks By Teachers, Librarians, And Parents, Megan Bright

Murray State Theses and Dissertations

Reading is a critical component of education. From learning to read, to eventually reading to learn, students and teachers rely upon texts to help make sense of the world around them. Methods and tools vary, but teachers, librarians, and parents can all agree that reading is a fundamental skill their students need. However, students are sometimes told to limit their use of audiobooks in favor of reading with their eyes. These limitations may prevent students from finding the books they love and also limit students’ opportunities to use books to learn about the world around them. This study sought to …

Student Support Teams In Title I Schools, Jennifer Michael

Murray State Theses and Dissertations

Student Support Teams (SST) are designed to be an innovative practice that supports the diverse needs of learners to help learners grow and be successful. This study will look at the impact that SSTs have on students in the Intermediate grades (3^rd-6^th Grade) at a Title I at-risk school. The research will analyze this question, does an SST positively impact student growth in reading and math in a highly diversified Title I School? The goal of the study is to analyze the impact of SST on the academic growth of students in specific focus groups. The study …

The Impact Of Intra-District, Open Enrollment School Choice On Students In A Selected Georgia School District, Elizabeth Miegel

Murray State Theses and Dissertations

This study aims to determine the impact of a Georgia school district’s intra-district, open enrollment Choice Program on students who participate compared to those who do not at a selected high school. Three research questions were developed: (1) does GPA differ between students who participate in school choice and students who do not (2) do SAT scores differ between students who participate in school choice and students who do not and (3) do AP test scores differ between students who participate in school choice and students who do not. The research questions were assessed through independent measures t-tests, with …

A Comparison Of Student Achievement On Agriculture Mechanics End Of Pathway Assessments Among Agriculture Programs In Georgia, William Burt

Murray State Theses and Dissertations

Agriculture education programs in Georgia are held to the same standards and evaluations regardless of their location, resource availability, and student clientele. For schools with agriculture mechanics programs specifically, students are expected to obtain proficient skills in welding, electrical wiring, woodworking, small engines, concrete, and machinery. Students' are expected to obtain skill sets in these various content areas, yet the equipment and resources available to the educator differs among schools. The experience that a student encounters also differs among schools. For instance, some schools have multiple agriculture pathways for a student to enroll in, while other schools have minimal. The …

Stakeholder Engagement: Challenges, Metrics, And Tactical Solutions In Higher Education Strategic Planning, Stephanie Miller Gillen

Murray State Theses and Dissertations

Effective stakeholder engagement is critical in higher education strategic planning, particularly as institutions face challenges such as changing demographics, technological advances, and evolving student expectations. This study explores the complexities of engaging diverse stakeholders, including students, faculty, community members, and governance, in the strategic planning process. Using a qualitative approach, the research examines how stakeholder engagement practices contribute to the development of strategic plans. The study emphasizes the importance of moving beyond superficial engagement to foster genuine collaboration and accountability, ultimately enhancing higher education institutions' adaptability. The research provides valuable insights for institutions aiming to enhance the efficacy of the …

Georgia Elementary Agriculture Education Pilot Program: An Extensive Look Into The History, Development, And Success, Christa Steinkamp

Murray State Theses and Dissertations

Georgia Elementary Agricultural Education (EAE) was formally introduced through the 2018 Senate Bill 330 (SB33); a two-part bill passed with unanimous support in both the House & the Senate. The first part of the bill included the three-component model of school-based agricultural education for high schools and middle schools which includes classroom/laboratory instruction, supervised agricultural experience, and leadership development through the National FFA Organization. The second part of SB330 introduced an elementary agricultural education (EAE) pilot program. The three-year pilot program started in the 2019-2020 school year with twenty-five schools; and concluded with thirty-one schools at the end of the …

Examining Stigma In Rural Mental Health Care Settings: A Mixed Methods Approach, Lainie Krumenacker

Murray State Theses and Dissertations

More than half of Americans will be diagnosed with a mental illness in their lifetime (CDC, 2021), yet stigma towards mental health affects both patients and providers. Although programs exist to address stigma, improve cultural competency among providers, and educate families on the importance of support, facilities are often limited on programs they provide due to allocation of resources and funds. Without a shift in treatment and programing, stigma will continue to impact patient care and outcome.

This study explored stigma among mental health providers in rural communities, while exploring potential differences in treatment among patients due to race. Mental …

Burnout Of Women In Agriculture, Ashley Rivers

Murray State Theses and Dissertations

Agriculture is considered one of the foundations of the United States. Women have played an important but often overlooked role in the history of agriculture. Despite many significant contributions, women have been left out of the history and advancements of agriculture. This research examined the lived experiences of 56 women who are involved in production agriculture and the factors that can contribute to stress and burnout in this area. By identifying how females view their roles and various aspects of their work, valuable insights can be gained into the experiences women face. Financial challenges and gender bias were identified as …

Exploring Teachers’ Perspectives On Integrating Gaming Into Agriculture Education Through Nasef Farmcraft, Carrie Settles Livers

Murray State Theses and Dissertations

With a growing population and a decrease in arable land, it is vital to prepare the next generation of agriculturalists. However, enrollment in agricultural education programs has been on the decline. Incorporating digital games into the classroom could serve to engage future students and encourage careers in agriculture. This study explored how Georgia agriculture teachers' technological and pedagogical knowledge influences their willingness to adopt digital games, specifically Georgia Farmcraft and Minecraft for Education, in their teaching. Additionally, it sought to identify demographic factors that might impact adoption and to determine effective professional development strategies to support teachers in using these …

Student Knowledge Of Sexual Assault Resources, Kali Forden

Murray State Theses and Dissertations

Despite the changes in policy and the endorsement of mandatory reporting among college students over time, less than half of survivors of sexual assault report the incident to police or university officials (Cantor et al., 2020). Further, while there is little research on the subject, Moore and Baker (2016) found that a student’s residence can influence whether they report a sexual assault. This project sought to extend this research to determine whether a student’s residence and knowledge of available resources effects the likelihood to report a hypothetical sexual assault. Additionally, it sought to determine the relationship between reporting a sexual …

K-5 Educators’ Perceptions Of Restorative Practices In St. Louis, Missouri, Jessica Ellen Finney

Murray State Theses and Dissertations

Restoration is any form of guidance or counsel toward right decision-making. One can be restored in an emotional or psychological way. The act of restoring emotional reactions back to logical thinking are the core teachings of this paper. The objective of this study was to analyze K-5 educators’ lived experiences, opinions, and perceptions in regard to this social and emotional restoration of teaching. This study advocates teacher perspectives in the art of restorative teaching and thinking for students they teach. This qualitative research study provides insight into the perceptions, opinions, and lived experiences of 10 educators regarding the implementation of …