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Investigating The Role Of Cd44 In Her2+ Breast Cancer Cells During Cancer Cell Redirection, Holly Caroline Jordan Campbell

All Dissertations

Despite a rise in survival rates of breast cancer patients due to advances in modern medicine, breast cancer is the most common type of cancer in women and is the second leading cause of cancer death in the United States. Early detection and a more personalized treatment of breast cancer has slowly improved patient outcomes. Nevertheless, there are impediments to better patient outcomes due to the complexity of the disease physiology. Therefore, there is a demand for a comprehensive understanding of cancer development as well as a need to understand tumor progression for enhanced therapies for patients.

Previous in vivo …

Normal Breast Tissues Harbour Rare Populations Of Aneuploid Epithelial Cells, Yiyun Lin, Junke Wang, Kaile Wang, Shanshan Bai, Aatish Thennavan, Yun Yan, Heba Elgamal, Emi Sei, Anna Casasent, Mitchell Rao, Chenling Tang, Asha S Multani, Jin Ma, Jessica Montalvan, Chandandeep Nagi, Sebastian Winocour, Bora Lim, Alastair Thompson, Nicholas Navin

Faculty, Staff and Student Publications

Aneuploid epithelial cells are common in breast cancer

Impact Of The Adenosine Receptor A2br Expressed On Myeloid Cells On Immune Regulation During Pregnancy, Stefanie Dietz, Janine Hebel, Jessica Rühle, Alisha Huff, Holger K Eltzschig, Christian F Poets, Natascha Köstlin-Gille

Faculty, Staff and Student Publications

During pregnancy, the maternal immune system must carefully balance protection against pathogens with tolerance toward the semiallogeneic fetus. Dysfunctions of the immune system can lead to severe complications such as preeclampsia, fetal growth restriction, or pregnancy loss. Adenosine plays a role in physiological processes and plasma‐level increase during pregnancy. The adenosine receptor A2B (A2BR), which is expressed on both, immune and nonimmune cells, is activated by high adenosine concentrations, achieved during pregnancy. We investigated the impact of A2BR expressed on myeloid cells on immune regulation during pregnancy using a mouse model with myeloid deficiency for A2BR. We demonstrate systemic changes …

Imipridones Inhibit Tumor Growth And Improve Survival In An Orthotopic Liver Metastasis Mouse Model Of Human Uveal Melanoma, Chandrani Chattopadhyay, Janos Roszik, Rajat Bhattacharya, Md Alauddin, Iqbal Mahmud, Mir Mustafa Ali, Varun Vijay Prabhu, Philip L Lorenzi, Bo Wei, Elizabeth Burton, Rohini R Morey, Rossana Lazcano, Michael A Davies, Sapna P Patel, Elizabeth A Grimm

Faculty, Staff and Student Publications

Background: Uveal melanoma (UM) is a highly aggressive disease with very few treatment options. We previously demonstrated that mUM is characterized by high oxidative phosphorylation (OXPHOS). Here we tested the anti-tumor, signaling and metabolic effects of imipridones, which are CLPP activators, which inhibit OXPHOS indirectly and have demonstrated safety in patients.

Methods: We assessed CLPP expression in UM patient samples. We tested the effects of imipridones (ONC201 and ONC212) on the growth, survival, signaling and metabolism of UM cell lines in vitro, and for therapeutic efficacy in vivo in UM liver metastasis models.

Results: CLPP expression was detected in primary …

Anti-Programmed Death Ligand 1 Plus Targeted Therapy In Anaplastic Thyroid Carcinoma: A Nonrandomized Clinical Trial, Maria E Cabanillas, Ramona Dadu, Renata Ferrarotto, Maria Gule-Monroe, Suyu Liu, Michelle D Williams, Jennifer R Wang, Charles Lu, Matthew Ning, Brian A Mckinley, Scott E Woodman, Dzifa Duose, Gary B Gunn, Naifa L Busaidy, Rare Tumor Initiative Team

Faculty, Staff and Student Publications

Importance: Anaplastic thyroid carcinoma (ATC) is a rare and lethal cancer. Although progress has been made in recent years in patients with mutated BRAF tumors, those who respond initially eventually die of their disease; furthermore, there are no approved therapies for non-BRAF mutated tumors.

Objective: To determine whether treatment with matched-targeted therapy plus immune checkpoint inhibitors were associated with improved overall survival (OS).

Design, setting, and participants: A phase 2 trial at a single center, tertiary institution with parallel cohorts, assigning treatment with targeted therapy according to the tumor mutation status. Patients with mutated BRAF V600E tumors received vemurafenib/cobimetinib plus …

Surfactant Protein-C Regulates Alveolar Type 2 Epithelial Cell Lineages Via The Cd74 Receptor, Krishan G Jain, Yang Liu, Runzhen Zhao, Preeti J Muire, Nan-Miles Xi

Faculty, Staff and Student Publications

Background: Deficiency of surfactant protein-C (SPC) increases susceptibility to lung infections and injury, and suppressed expression of SPC has been associated with the severity of acute respiratory distress syndrome (ARDS). Alveolar type 2 epithelial cells (AT2) are critical for maintenance and repair of the lung. However, the role of the SPC in the regulation of AT2 cell lineage and the underlying mechanisms are not completely understood.

Methods: This study aimed to investigate the mechanisms by which SPC regulates AT2 lineages. Sftpc-/- mice were used to model the SPC deficiency in ARDS patients. We utilized three-dimensional (3D) organoids to compare AT2 …

Explainable Artificial Intelligence And Domain Adaptation For Predicting Hiv Infection With Graph Neural Networks, Evan Yu, Jingcheng Du, Yang Xiang, Xinyue Hu, Jingna Feng, John A Schneider, Kayo Fujimoto, Cui Tao

Faculty, Staff and Student Publications

OBJECTIVE: Investigation of explainable deep learning methods for graph neural networks to predict HIV infections with social network information and performing domain adaptation to evaluate model transferability across different datasets.

METHODS: Network data from two cohorts of younger sexual minority men (SMM) from two U.S. cities (Chicago, IL, and Houston, TX) were collected between 2014 and 2016. Feature importance from graph attention network (GAT) models were determined using GNNExplainer. Domain adaptation was performed to examine model transferability from one city dataset to the other dataset, training with 100% of the source dataset with 30% of the target dataset and prediction …

Mtor Maintains Endothelial Cell Integrity To Limit Lung Vascular Injury, Michelle Warren Millar, Rauf A Najar, Spencer A Slavin, Mohammad Shadab, Imran Tahir, Xin Lin, Terry W Wright, David A Dean, Fabeha Fazal, Arshad Rahman

Faculty, Staff and Student Publications

The functional and structural integrity of the endothelium is essential for vascular homeostasis. Loss of barrier function in quiescent and migratory capacity in proliferative endothelium causes exuberant vascular permeability, a cardinal feature of many inflammatory diseases including acute lung injury (ALI). However, the signals governing these fundamental endothelial cell (EC) functions are poorly understood. Here, we identify mechanistic target of rapamycin (MTOR) as an important link in preserving the barrier integrity and migratory/angiogenic responses in EC and preventing lung vascular injury and mortality in mice. Knockdown of MTOR in EC altered cell morphology, impaired proliferation and migration, and increased endocytosis …

Promotion And Tenure For Medical Physicists Should Be Based On Article Specific Measures And Not On Journal Impact Factor, Samantha Hedrick, Jinzhong Yang, Yi Rong

Faculty, Staff and Student Publications

No abstract provided.

The Prosecutor Vacancy Crisis, Adam M. Gershowitz

Faculty Publications

There is a prosecutor vacancy crisis in the United States. Prosecutors are quitting in droves and there are few applicants to replace them. In Houston and Los Angeles, more than 15% of prosecutor positions are open. In Detroit, the vacancy rate exceeds 20%. In Alameda, 25% of prosecutor positions are empty. And in Miami, a staggering 33% of prosecutor positions are unfilled. The situation is equally dire in many large and small counties across the nation.

Drawing on data and interviews from more than two-dozen district attorney’s offices, this Article documents how low salaries, massive caseloads, lack of remote work …

Immunopeptidomic Mhc-I Profiling And Immunogenicity Testing Identifies Tcj2 As A New Chagas Disease Mrna Vaccine Candidate, Jungsoon Lee, Junfei Wei, Nelufa Islam, Brian Keegan, William K Russell, Cristina Poveda, Maria Jose Villar, Kathryn Jones, Maria Elena Bottazzi, Peter Hotez, Edwin Tijhaar, Jeroen Pollet

Faculty, Staff and Students Publications

Trypanosoma cruzi is a protozoan parasite that causes Chagas disease. Globally 6 to 7 million people are infected by this parasite of which 20-30% will progress to develop Chronic Chagasic Cardiomyopathy (CCC). Despite its high disease burden, no clinically approved vaccine exists for the prevention or treatment of CCC. Developing vaccines that can stimulate T. cruzi-specific CD8+ cytotoxic T cells and eliminate infected cells requires targeting parasitic antigens presented on major histocompatibility complex-I (MHC-I) molecules. We utilized mass spectrometry-based immunopeptidomics to investigate which parasitic peptides are displayed on MHC-I of T. cruzi infected cells. Through duplicate experiments, we identified an …

Targeting The Glutamine-Arginine-Proline Metabolism Axis In Cancer, Di Wang, Jiang-Jie Duan, Yu-Feng Guo, Jun-Jie Chen, Tian-Qing Chen, Shi-Cang Yu

Faculty, Staff and Student Publications

Metabolic abnormalities are an important feature of tumours. The glutamine-arginine-proline axis is an important node of cancer metabolism and plays a major role in amino acid metabolism. This axis also acts as a scaffold for the synthesis of other nonessential amino acids and essential metabolites. In this paper, we briefly review (1) the glutamine addiction exhibited by tumour cells with accelerated glutamine transport and metabolism; (2) the methods regulating extracellular glutamine entry, intracellular glutamine synthesis and the fate of intracellular glutamine; (3) the glutamine, proline and arginine metabolic pathways and their interaction; and (4) the research progress in tumour therapy …

Healthcare Disparities, Screening, And Molecular Testing In The Changing Landscape Of Non-Small Cell Lung Cancer In The United States: A Review, Razelle Kurzrock, Aadel A Chaudhuri, David Feller-Kopman, Narjust Florez, Jed Gorden

Faculty, Staff and Student Publications

Inequitable access to care continues to hinder improvements in diagnosis and treatment of lung cancer. This review describes healthcare disparities in the changing landscape of non-small cell lung cancer (NSCLC) in the United States, focusing on racial, ethnic, sex-based, and socioeconomic trends. Furthermore, strategies to address disparities, overcome challenges, and improve patient outcomes are proposed. Barriers exist across lung cancer screening, diagnosis, and treatment regimens, varying by sex, age, race and ethnicity, geography, and socioeconomic status. Incidence and mortality rates of lung cancer are higher among Black men than White men, and incidences in young women are substantially greater than …

Low Intensity Mechanical Signals Promote Proliferation In A Cell-Specific Manner: Tailoring A Non-Drug Strategy To Enhance Biomanufacturing Yields, M Ete Chan, Christopher Ashdown, Lia Strait, Sishir Pasumarthy, Abdullah Hassan, Chanpreet Singh, Gabriel Pagnotti, Omor Khan, Gunes Uzer, Clinton T Rubin

Faculty, Staff and Student Publications

Biomanufacturing relies on living cells to produce biotechnology-based therapeutics, tissue engineering constructs, vaccines, and a vast range of agricultural and industrial products. With the escalating demand for these bio-based products, any process that could improve yields and shorten outcome timelines by accelerating cell proliferation would have a significant impact across the discipline. While these goals are primarily achieved using biological or chemical strategies, harnessing cell mechanosensitivity represents a promising - albeit less studied - physical pathway to promote bioprocessing endpoints, yet identifying which mechanical parameters influence cell activities has remained elusive. We tested the hypothesis that mechanical signals, delivered non-invasively …

Low Toxicity Of Dissolved Silver From Silver-Coated Titanium Dental Implants To Human Primary Osteoblast Cells, Ranj Nadhim Salaie, Alexandros Besinis, Christopher Tredwin, Richard D Handy

School of Engineering, Computing and Mathematics

The controlled release of silver as a biocide from Ag-coated medical implants is desirable. However, the biocompatibility of Ag leachates is poorly understood. This study investigated the toxicity of silver released from the silver plated titanium implants to human primary osteoblast cells; and the effect of cell culture medium on the silver speciation and bioavailability.

METHODS: Ti6Al4V discs were coated with Ag nanoparticles (NPs), silver plus hydroxyapatite (HA) nanoparticles (Ag+nHA), or Ag NPs plus microparticles (Ag+mHA). Primary human osteoblast cells were exposed to the leachates from the various discs for up to 7 days.

RESULTS: the total Ag concentrations released …

Telehealth In Emergency Medicine Residency Training: A Model Curriculum, Bruce M Lo, Samuel D Luber

Faculty, Staff and Student Publications

Emergency physicians are well‐positioned to take a leadership role in telehealth, particularly in emerging categories such as triage, direct acute unscheduled care, and virtual observation. However, the growth of telehealth has outpaced curricular development in emergency medicine (EM) residency programs. This manuscript presents a model longitudinal telehealth curriculum, developed by the consensus of education experts, including representatives from the telehealth interest groups from EM's two primary specialty societies: the American College of Emergency Physicians and the Society for Academic Emergency Medicine. The curriculum describes overarching goals and components that may serve as a foundation for individual institutions seeking to train …

Prevention Is Better Than Cure: Rethinking Court Behaviour And Design, De Maartje Visser

Research Collection Yong Pung How School Of Law

Using a relational dynamics perspective, this commentary considers how to buttress courts' ability to practice their role as guardians of the constitution. It suggests that we should pay more attention to empowering the general public to provide meaningful social support for courts through constitutional literacy efforts. Such literacy efforts should focus on the place of the court within the separation of powers and the value of judicial independence. At the same time, it may be prudent to redact the list of tasks given to constitutional courts and divest responsibility for some activities to other institutions.

Alternative Agrifood Systems And The Economic Sustainability Of Farmers' Cooperatives: The Chinese Experience, Zhanping Hu, Qian Forrest Zhang

Research Collection School of Social Sciences

Most registered farmers' cooperatives in China are de facto private enterprises; while many bring economic gains to farmers, they do not function as cooperatives. Among the small minority that started as authentic cooperatives, however, most struggled to provide economic benefits to members, unable to achieve economic sustainability. The failure of true farmers' cooperatives in China has been widely studied; the success of the few that did become economically sustainable, however, remains poorly understood. Using a sample of 70 farmers' cooperatives across the country and comparing three “extreme cases”—cooperatives that are both authentic and economically successful—with the rest, this study argues …

Computer Use Frequency And Cardiovascular Health In Middle-Aged And Older Adults, Meilan Hu, Shu Fen Diong, K. T. A. Sandeeshwara Kasturiratna, Andree Hartanto

Research Collection School of Social Sciences

With increased computer usage amongst midlife and older adults, concerns are emerging with regards to the potential adverse health effects of computer use given the sedentary habits it may encourage. The current study aims to investigate the relationship between computer use and cardiovascular risk in midlife and older adults. From the National Survey of Midlife Development in the United States II: Biomarker Project (2004–2009) and the National Survey of Midlife Development in the United States (MIDUS II), 2004–2006, we examined five cardiovascular risk biomarkers—high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, interleukin-6, and C-reactive protein—in relation to self-reported general …

(In)Visible China: Understanding Chinese Global Orders, Hassan Karrar, William A. Callahan, Carwyn Morris, Stephen Whiteman

Research Collection School of Social Sciences

Where is China going? What does its alternative global order look like? We hear a lot about China’s grand projects like the Belt and Road Initiative, but how are they experienced on the ground in Africa, South America, and Asia?The Chinese Global Orders project brings together twenty-two scholars from five continents to explore these questions. As these four commentaries show, it seeks to pluralize the discussion by exploring “Chinese” beyond the PRC nation-state, “Global” as a space beyond the international, and “Orders” as a plural set of norms.The project seeks to do more than just describe Global China’s material impact, …

Assessing Divergence And Convergence In The Design Process: Results From Two Experiments, Arlindo Silva, Tamas Makany, Matt Chiu

Research Collection Lee Kong Chian School Of Business

It is often assumed that the design process comprises divergent and convergent thinking cycles. Although many authors studied the design process in depth, a quantification of divergence and convergence is yet to be established. This paper attempts to quantify divergence and convergence in the design process for teams of design students. Student submissions from two independent design-related courses from two Universities are analysed for divergent and convergent thinking. Such an approach uses Natural Language Processing to harvest existing student submissions in written documents and converts them into a 'real-time' dashboard for feedback that can be used in multiple ways: searching …

Phypo: Priority-Based Hybrid Task Partitioning And Offloading In Mobile Computing Using Automated Machine Learning, Shehr Bano, Ghulam Abbas, Muhammad Bilal, Ziaul Haq Abbas, Zaiwar Ali, Muhammad Waqas

Research outputs 2022 to 2026

With the increasing demand for mobile computing, the requirement for intelligent resource management has also increased. Cloud computing lessens the energy consumption of user equipment, but it increases the latency of the system. Whereas edge computing reduces the latency along with the energy consumption, it has limited resources and cannot process bigger tasks. To resolve these issues, a Priority-based Hybrid task Partitioning and Offloading (PHyPO) scheme is introduced in this paper, which prioritizes the tasks with high time sensitivity and offloads them intelligently. It also calculates the optimal number of partitions a task can be divided into. The utility of …

What Journalism Feels Like: Considering The Body Of The Journalist, Mark Deuze, Laura Glitsos

Research outputs 2022 to 2026

All journalists have bodies. This commonsensical fact acquires more currency every day as the work of reporters and editors becomes automated, robotized, and taken over by (generative) artificial intelligence. The embodied nature of news work matters, not in the least because of the personal attachment practitioners have to what journalism is, or should be. However, in the rich history of journalism studies, bodily perspectives are remarkably absent—beyond descriptions of journalists as sociodemographic profiles. In this essay, we explore various theoretical frameworks to bring the body back into the study and practice of journalism. In our argument, we apply the insights …

Corrosion Behavior Of Laser Powder Bed Fusion Additive Manufacturing Produced Tini Alloy By Micro-Arc Oxidation, Xin Zhao, Yicheng Liu, Chenfan Jia, Hao Chang, Wei Zhang, Yun Bai, Shujun Li, Lai-Chang Zhang, Wei Yuan

Research outputs 2022 to 2026

To improve the corrosion resistance of TiNi alloy fabricated by laser powder bed fusion (LPBF), a porous oxidation layer was synthesized by micro-arc oxidation in a sodium aluminate and sodium silicate electrolyte. The influences of the applied voltage and the processing time on the morphology of oxidation layer were investigated, and the corrosion behavior of the oxidation layer in artificial saliva was evaluated and compared with that of the as-fabricated LPBF alloy. The results indicate that, as increasing the applied voltage and the processing time, the oxidation layer becomes uniform and integrated. The optimum parameters are with an applied voltage …

Fomo And The Ico: The Changing Salience Of Quality Signals, Simon J.D. Schillebeeckx, Sandzhar Tazhibaev, Johannes Gartner

Research Collection Lee Kong Chian School Of Business

The manuscript investigates whether the individual personality trait, Fear of Missing Out (FOMO)—typically considered negative—influences the willingness of individuals to contribute to Initial Coin Offerings (ICOs), a phenomenon that emerged after the blockchain revolution. We conducted both qualitative and quantitative work in this space and present the results of an international survey, including a conjoint experiment. Theoretically, we anchor our study in signaling theory and propose that signal valence (the positive or negative interpretation of a signal) can diverge from signal intent. Specifically, we find that candidate ICO funders with strong FOMO behave predictably irrationally. They are more likely to …

The Role Of Porphyromonas Gingivalis In Inducing Il-6 Macrophages Expression In Ovaries Of Periodontitis Models, Agustin Ws. Dharmayanti, Ernie M. Setiawatie, Hendy Hendarto

Journal of Dentomaxillofacial Science

Objective: This study aimed to determine whether P. gingivalis induction in the gingival sulcus increased IL-6 expression by macrophages in the ovaries.

Material and Methods: This research was a laboratory experiment using a post-test-only control group design, using female white rats strain Sprague Dawley, six weeks old and in the estrous phase. They were divided into the control and treatment groups. The animal models were induced with P. gingivalis in the mesial gingival sulcus of their first right and left lower jaw molars, with 0.05 ml every three days for 19 days. On 0, 7, 14 days after bacteria induction, …

Article Iii Standing In Federal Prosecutions Of “Victimless Crimes”, Ryan H. Nelson

Fordham Law Review Online

Plaintiffs in federal court bear the burden of proving their standing, as Article III permits inferior federal courts, after Congressional authorization, to exercise jurisdiction over “Cases” and “Controversies” alone. From these constitutional terms of art—“Cases” and “Controversies”—we derive the familiar case-or-controversy requirement of standing, including injury. These terms of art authorize Congress to empower the inferior federal courts to hear civil and criminal actions alike, but federal prosecutors have never been similarly burdened with proving the standing of the United States in federal court, including that the United States has suffered injury. This Essay examines that lapse and contends that …

Cover And Forewords, C. Nicholas Raphael

The Geographical Bulletin

Cover and Forewords

Volume 43-1 Complete Issue, C. Nicholas Raphael

The Geographical Bulletin

Volume 43-1 Complete Issue

Between Africa And The Abyss: Globalization, Media, And The Invisibility Of A Continent, Julie A. Silva, Elvin K. Wyly

The Geographical Bulletin

Mass communication, perception, and mental maps are pervasive themes in human geography. Yet the role of globalization on our collective mental maps remains poorly understood, raising critical questions of theory and policy as flows of capital, people, and ideas continue to blur the boundaries between local and international events. This paper analyzes these themes in the context of subSaharan Africa, focusing specifically on the civil war in Sierra Leone. The nature of armed conflict in Africa has evolved considerably in recent decades, and the globalization of Western media has altered the way the region has been portrayed. As publishers, editors, …