Digital Commons Network^™

Single-Cell Analysis Identifies Distinct Macrophage Phenotypes Associated With Prodisease And Proresolving Functions In The Endometriotic Niche., Yasmin Henlon, Kavita Panir, Iona Mcintyre, Chloe Hogg, Priya Dhami, Antonia O Cuff, Anna Senior, Niky Moolchandani-Adwani, Elise T Courtois, Andrew W Horne, Matthew Rosser, Sascha Ott, Erin Greaves

Faculty Research 2024

Endometriosis negatively impacts the health-related quality of life of 190 million women worldwide. Novel advances in nonhormonal treatments for this debilitating condition are desperately needed. Macrophages play a vital role in the pathophysi- ology of endometriosis and represent a promising therapeutic target. In the current study, we revealed the full transcriptomic complexity of endometriosis-associated macrophage subpopulations using single-cell analyses in a preclinical mouse model of experimental endometriosis. We have identified two key lesion-resident populations that resemble i) tumor-associated macrophages (characterized by expression of Folr2, Mrc1, Gas6, and Ccl8+) that promoted expression of Col1a1 and Tgfb1 in human endometrial stromal cells …

The Estrogen Receptor-Related Orphan Receptors Regulate Autophagy Through Tfeb, Mckenna Losby, Matthew Hayes, Aurore Valfort, Danesh H Sopariwala, Ryan Sanders, John K Walker, Weiyi Xu, Vihang A Narkar, Lilei Zhang, Cyrielle Billon, Thomas P Burris

Faculty, Staff and Students Publications

Autophagy is an essential self-degradative and recycling mechanism that maintains cellular homeostasis. Estrogen receptor-related orphan receptors (ERRs) are fundamental in regulating cardiac metabolism and function. Previously, we showed that ERR agonists improve cardiac function in models of heart failure and induce autophagy. Here, we characterized a mechanism by which ERRs induce the autophagy pathway in cardiomyocytes. Transcription factor EB (TFEB) is a master regulator of the autophagy-lysosome pathway and has been shown to be crucial regulator of genes that control autophagy. We discovered that TFEB is a direct ERR target gene whose expression is induced by ERR agonists. Activation of …

Footprint Tools Tiptoeing Towards Nitrogen Sustainability, James N. Galloway, Elizabeth A. Castner, Elizabeth S M Dukes, Jessica Fox, Allison M. Leach

Faculty Publications

As we face multiple environmental crises (e.g. climate change, nutrient pollution, freshwater scarcity), there is often a disconnect between an entity’s activities and the pollution resulting from that activity. Footprint tools help address this disconnect. They are effective in educating people, institutions and communities on how their resource use results in environmental pollution and what we can do to moderate that pollution. These tools connect an entity’s activities with the associated pollution. Footprint tools are also constantly evolving. As we better understand how to best estimate emissions—especially from sources further removed like those from the supply chain—the guidance and methods …

Footprint Tools Tiptoeing Towards Nitrogen Sustainability, James N. Galloway, Elizabeth A. Castner, Elizabeth S M Dukes, Jessica Fox, Allison M. Leach

Faculty Publications

As we face multiple environmental crises (e.g. climate change, nutrient pollution, freshwater scarcity), there is often a disconnect between an entity’s activities and the pollution resulting from that activity. Footprint tools help address this disconnect. They are effective in educating people, institutions and communities on how their resource use results in environmental pollution and what we can do to moderate that pollution. These tools connect an entity’s activities with the associated pollution. Footprint tools are also constantly evolving. As we better understand how to best estimate emissions—especially from sources further removed like those from the supply chain—the guidance and methods …

Vglut2-Based Glutamatergic Signaling In Central Noradrenergic Neurons Is Dispensable For Normal Breathing And Chemosensory Reflexes, Yuan Chang, Savannah Lusk, Andersen Chang, Christopher S Ward, Russell S Ray

Faculty, Staff and Students Publications

Central noradrenergic (NA) neurons are key constituents of the respiratory homeostatic network. NA dysfunction is implicated in several developmental respiratory disorders including Congenital Central Hyperventilation Syndrome (CCHS), Sudden Infant Death Syndrome (SIDS), and Rett Syndrome. The current unchallenged paradigm in the field, supported by multiple studies, is that glutamate co-transmission in subsets of central NA neurons plays a role in breathing control. If true, NA-glutamate co-transmission may also be mechanistically important in respiratory disorders. However, the requirement of NA-derived glutamate in breathing has not been directly tested and the extent of glutamate co-transmission in the central NA system remains uncharacterized. …

Host-Derived Ceacam-Laden Vesicles Engage Enterotoxigenic Escherichia Coli For Elimination And Toxin Neutralization, Alaullah Sheikh, Debayan Ganguli, Tim J Vickers, Marjahan Akhtar, Nazia Khatoon, Bipul Setu, Supratim Basu, Wandy L Beatty, James M Fleckenstein, Et Al.

2020-Current year OA Pubs

Enterotoxigenic

Drivers Of Coral Reef Benthic Changes And Implications On Ecosystem Functioning And Services, Miriam Reverter

School of Biological and Marine Sciences

Increasing anthropogenic stressors will likely result in many coral reefs undergoing compositional and functional benthic changes, including towards non-hard coral dominated communities. The decline of reef building species and the increase of alternative (i.e., non reef-building) organisms profoundly affect the functioning of coral reefs and the services provided, but this remains poorly understood, especially for non-algal alternative communities. Here, we propose a standardised framework to study coral reef benthic changes and review the knowledge on coral reef shifts and their implications on coral reef functioning and service provisioning using this framework, which contains the five following elements: 1) contextual information, …

The George-Anne Inkwell Edition, Georgia Southern University

The Inkwell

No abstract provided.

Creb-Binding Protein/P300 Bromodomain Inhibition Reduces Neutrophil Accumulation And Activates Antitumor Immunity In Triple-Negative Breast Cancer, Xueying Yuan, Xiaoxin Hao, Hilda L Chan, Na Zhao, Diego A Pedroza, Fengshuo Liu, Kang Le, Alex J Smith, Sebastian J Calderon, Nadia Lieu, Michael J Soth, Philip Jones, Xiang Hf Zhang, Jeffrey M Rosen

Faculty, Staff and Students Publications

Tumor-associated neutrophils (TANs) have been shown to promote immunosuppression and tumor progression, and a high TAN frequency predicts poor prognosis in triple-negative breast cancer (TNBC). Dysregulation of CREB-binding protein (CBP)/P300 function has been observed with multiple cancer types. The bromodomain (BRD) of CBP/P300 has been shown to regulate its activity. In this study, we found that IACS-70654, a selective CBP/P300 BRD inhibitor, reduced TANs and inhibited the growth of neutrophil-enriched TNBC models. In the bone marrow, CBP/P300 BRD inhibition reduced the tumor-driven abnormal differentiation and proliferation of neutrophil progenitors. Inhibition of CBP/P300 BRD also stimulated the immune response by inducing …

Adamts12 Promotes Fibrosis By Restructuring Extracellular Matrix To Enable Activation Of Injury-Responsive Fibroblasts, Konrad Hoeft, Benjamin D. Humphreys, Et Al.

2020-Current year OA Pubs

Fibrosis represents the uncontrolled replacement of parenchymal tissue with extracellular matrix (ECM) produced by myofibroblasts. While genetic fate-tracing and single-cell RNA-Seq technologies have helped elucidate fibroblast heterogeneity and ontogeny beyond fibroblast to myofibroblast differentiation, newly identified fibroblast populations remain ill defined, with respect to both the molecular cues driving their differentiation and their subsequent role in fibrosis. Using an unbiased approach, we identified the metalloprotease ADAMTS12 as a fibroblast-specific gene that is strongly upregulated during active fibrogenesis in humans and mice. Functional in vivo KO studies in mice confirmed that Adamts12 was critical during fibrogenesis in both heart and kidney. …

Dual-Peptide Functionalized Hydrogel For Enhancing Biosynthesis And Immunomodulation In Primary And Stem Cells For Therapeutic Applications, Xiaohong Tan

McKelvey School of Engineering Graduate Student Theses & Dissertations

Intervertebral disc (IVD) degeneration significantly contributes to disability and the economic burden of musculoskeletal diseases. Early changes in IVD degeneration include reduced cellularity and altered phenotype in nucleus pulposus (NP) cells, leading to diminished capacity for extracellular matrix (ECM) and glycosaminoglycan production. Cell supplementation, particularly with bone marrow-derived human mesenchymal stem cells (MSCs), has been explored to promote ECM synthesis and repair. MSCs offer potential for either direct cell replacement or by secreting factors to support disc repair by restoring biosynthetic activity of remaining endogenous NP cells and ameliorating inflammation. Biomaterials have been extensively studied to preserve and restore the …

Dual-Peptide Functionalized Hydrogel For Enhancing Biosynthesis And Immunomodulation In Primary And Stem Cells For Therapeutic Applications, Xiaohong Tan

McKelvey School of Engineering Graduate Student Theses & Dissertations

Intervertebral disc (IVD) degeneration significantly contributes to disability and the economic burden of musculoskeletal diseases. Early changes in IVD degeneration include reduced cellularity and altered phenotype in nucleus pulposus (NP) cells, leading to diminished capacity for extracellular matrix (ECM) and glycosaminoglycan production. Cell supplementation, particularly with bone marrow-derived human mesenchymal stem cells (MSCs), has been explored to promote ECM synthesis and repair. MSCs offer potential for either direct cell replacement or by secreting factors to support disc repair by restoring biosynthetic activity of remaining endogenous NP cells and ameliorating inflammation. Biomaterials have been extensively studied to preserve and restore the …

Detecting Altered Hepatic Lipid Oxidation By Mri In An Animal Model Of Masld, Marc Mcleod, Mario C Chang, Anna Rushin, Mukundan Ragavan, Rohit Mahar, Gaurav Sharma, Arshee Badar, Anthony Giacalone, Max E Glanz, Vinay R Malut, Dalton Graham, Nishanth E Sunny, James A Bankson, Kenneth Cusi, Matthew E Merritt

Faculty, Staff and Student Publications

Metabolic dysfunction-associated steatotic liver disease (MASLD) prevalence is increasing annually and affects over a third of US adults. MASLD can progress to metabolic dysfunction-associated steatohepatitis (MASH), characterized by severe hepatocyte injury, inflammation, and eventual advanced fibrosis or cirrhosis. MASH is predicted to become the primary cause of liver transplant by 2030. Although the etiology of MASLD/MASH is incompletely understood, dysregulated fatty acid oxidation is implicated in disease pathogenesis. Here, we develop a method for estimating hepatic β-oxidation from the metabolism of [D

Gut Phageome In Mexican Americans: A Population At High Risk For Metabolic Dysfunction-Associated Steatotic Liver Disease And Diabetes, Suet-Ying Kwan, Caroline M Sabotta, Lorenzo R Cruz, Matthew C Wong, Nadim J Ajami, Joseph B Mccormick, Susan P Fisher-Hoch, Laura Beretta

Faculty, Staff and Student Publications

Mexican Americans are disproportionally affected by metabolic dysfunction-associated steatotic liver disease (MASLD), which often co-occurs with diabetes. Despite extensive evidence on the causative role of the gut microbiome in MASLD, studies determining the involvement of the gut phageome are scarce. In this cross-sectional study, we characterized the gut phageome in Mexican Americans of South Texas by stool shotgun metagenomic sequencing of 340 subjects, concurrently screened for liver steatosis by transient elastography. Inter-individual variations in the phageome were associated with gender, country of birth, diabetes, and liver steatosis. The phage signatures for diabetes and liver steatosis were subsequently determined. Enrichment of …

Loss Of P53 And Smad4 Induces Adenosquamous Subtype Pancreatic Cancer In The Absence Of An Oncogenic Kras Mutation, Daowei Yang, Xinlei Sun, Rohan Moniruzzaman, Hua Wang, Citu Citu, Zhongming Zhao, Ignacio I Wistuba, Huamin Wang, Anirban Maitra, Yang Chen

Faculty, Staff and Student Publications

Pancreatic cancer is associated with an oncogenic KRAS mutation in approximately 90% of cases. However, a non-negligible proportion of pancreatic cancer cases harbor wild-type KRAS (KRAS-WT). This study establishes genetically engineered mouse models that develop spontaneous pancreatic cancer in the context of KRAS-WT. The Trp53

Systematic Review On Isolation, Purification, Characterization, And Industrial Applications Of Thermophilic Microbial Α- Amylases, Rugaiyah A. Arfah, Sarlan Sarlan, Abdul Karim, Anita Anita, Ahyar Ahmad, Paulina Taba, Harningsih Karim, Siti Halimah Larekeng, Dorothea Agnes Rampisela, Rusdina Bte Ladju

Karbala International Journal of Modern Science

The α-amylase enzyme, sourced from diverse organisms, including plants, animals, and bacteria, plays a crucial role in multiple industries, notably food processing sectors like cakes, fruit juices, and starch syrup. Research identifies thermophilic organisms as prime sources of this enzyme thriving at temperatures ranging from 41°C to 122°C. The enzyme purification was carried out using liquid-liquid extraction, which involved the exchange of substances between two liquid phases that were immiscible or partially soluble. The optimal temperature for α-amylase was 45 to 90°C. The best pH for bacterial and fungal α-amylases ranged from 5.0 to 10.5 and 5.0 to 9.0. Based …

Wmdds Bulletin Summer 2020

West Michigan District Dental Society Bulletin

In the Summer 2020 issue, the reader will find the following feature articles:

• Ask an Expert: What Does This New Legislation Around PPP Mean for Me?
• COVID-19 Pandemic Results in Severe Operating Room Access Limitations for Pediatric Dentists

Also included are your monthly tripartite update, news from West Michigan counties, the President's Message and Editor's Letter.

Wmdds Bulletin Holiday 2020

West Michigan District Dental Society Bulletin

In the Holiday 2020 issue, the reader will find the following feature articles:

• Evidence-Based Dentistry: Learning from a Local Expert
• Dentists Doing Good: Dr. Dan Armstrong, Exalta Health
• Dentists Beyond the Chair: Chairman of Board of Directors for Pankey Institute

Also included are your monthly tripartite update, news from West Michigan counties, the President's Message and Editor's Letter.

Wmdds Bulletin Fall 2020

West Michigan District Dental Society Bulletin

In the Fall 2020 issue, the reader will find the following feature articles:

• Looking to Leadership: Personal Experiences During the Pandemic
• Dentists Doing Good: WMDF Golf Outing
• Dentists Beyond the Chair: Maintaining a Pollinator Habitat and 52 Acre Farm
• 2020 WMDDS Directory Corrections/Additions

Also included are your monthly tripartite update, news from West Michigan counties, the President's Message and Editor's Letter.

El Único Pecado De Chepita Rodriguez, Maria G. Vielma

Regeneración: A Xicanacimiento Studies Journal

Cuento.

Myth And Monument In Old Town Albuquerque: Southwest Pietà And The War Of Presiding Histories, Eric Castillo

Regeneración: A Xicanacimiento Studies Journal

Luis Jiménez’s Southwest Pietà (1984) intended to combat cultural amnesia that obscured Native Americans’ and Mexicans’ contributions to the state. Jiménez’s Pietà sought to counter the iconography that shaped New Mexico’s colonialist heritage. But Old Town Albuquerque shrouds Native American and Mexican contributions to the region. Albuquerque’s public art has often been deployed as a wedge to write and rewrite narratives about land inhabitants, but the city’s public art tells a powerful story about race and place in New Mexico. This essay explores the socio-historical battle of land memorialization in Old Town Albuquerque and provides a geo-racial perspective about the …

The Queer Body And The Obstructed Gaze: Chicanx Performance Artists And The Power Of Self-Objectification, Tracie N. Amend

Regeneración: A Xicanacimiento Studies Journal

The Queer Body and the Obstructed Gaze: Chicanx Performance Artists and the Power of Self-Objectification

This article examines the ways in which four queer Chicanx performance artists have manipulated and obstructed the spectator’s gaze as a method for activating their abjection, performing self-objectification or fetishization, or asserting agency. As seminal Chicanx and queer performance artists, Luis Alfaro and Nao Bustamante set the stage for identity exploration through the corporeal and figurative display of excess: they invite in the spectator’s gaze to look at the queer brown body and challenge them to resist a reductive framing of their othered voluptuousness. In …

Teaching Manita/O New Mexico To Knowledge And Power Curators: Epistemologies Of The Manita Nuevo Mexicans/O, Keith E. Sanchez

Regeneración: A Xicanacimiento Studies Journal

This essay examines the cultural ecologies of Manita/o New Mexico and the educational model of La Academia de La Nueva Raza developed therein. The conclusion section offers a brief synthesis of applied pedagogical models of CCM and a sampling of culturally relevant experiential curricula concepts authored by me. The academic aim is to inform power and knowledge curators working within the Manita/o expression of the Chicana/o diaspora. The objective of this text is to create an infrastructural guide for further study and curricula development. The curriculum directed to teach and inspire professors, teachers, and public-policy makers are intended to …

Julie And Her Chickens, Lorissa Rinehart

PoemMemoirStory (literary magazine)

pp. 126-136

Little Creatures, Jane Kokernak

PoemMemoirStory (literary magazine)

pp. 82-92

To Famous Cases Of Syphilis, Re'lynn Hansen

PoemMemoirStory (literary magazine)

pp. 62-73

Milton's Captive Audience: Teaching Paradise Lost In A Maximum Security Prison, Alison A. Chapman

PoemMemoirStory (literary magazine)

pp. 49-61

Uncommon Renal Manifestation And Persistent Hiccups In A Patient With Coexisting Comorbidites: A Case Of Complicated Brucellosis, Meghna Neog, Weena Stanley, Shashikala .

Vedam- 2024, The Academic Fest and Undergraduate Medical Conference of KMC, Manipal

No abstract provided.

James E. Reagan, Dds: The Apple Doesn’T Fall Far From The Tree

West Michigan District Dental Society Bulletin

West Michigan’s Jim Reagan was born James Edward Reagan on October 22nd, 1957, and he grew up in Lowell, Michigan. Jim was the fourth of ten Reagan children born to Bob (Robert Reagan, DDS) and Carol Reagan. Carol Reagan was an RN from Marquette, but her time was filled by being a homemaker. Jim’s dad, Bob Reagan, practiced as a dentist in Lowell, MI from 1953 until 1993. In fact, Bob Reagan was recognized with the Silent Bell in 1993. Jim and Bob are the first multi-generational family members to share that honor.

Wmdds Bulletin Spring 2024

West Michigan District Dental Society Bulletin

In the Spring 2024 issue, the reader will find the following feature articles:

• Advocacy Avenue: ADPAC and you
• Opportunities in Grand Rapids for Oral Surgeons and Dentists with Advanced Training
• James E. Reagan, DDS: The Apple Doesn't Fall Far from the Tree
• Silent Bell Awards Night and Celebration Highlights

Also included are your monthly tripartite update, news from West Michigan counties, the President's Message and Editor's Letter.