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Role Of Human Intermediate Form Prl Receptor I-Tail In The Pathogenesis Of Breast Cancer, Shanwei Shen

Theses and Dissertations

Breast cancer continues to be the second most common cancer in women in the United States. The neuroendocrine hormone human prolactin (hPRL) plays an important role in normal mammary gland development and growth, stimulating normal breast tissue proliferation and differentiation. Considerable evidence has shown that hPRL is involved in the malignant transformation of human breast tissue. hPRL enhances viability, invasiveness, and proliferation of breast cancer cells in vitro. Evidence from epidemiologic and genetic studies also implicates role of hPRL in the pathogenesis of breast cancer. Its cognate receptor the human prolactin receptor (hPRLr) is required for the action of hPRL. …

Fat And Water Content Effects Of Three Food Substrates (Ground Veal, Ground Beef, And Beef Liver) On Lucilia Sericata (Diptera: Calliphoridae) Development And Mortality, Nicole Dailey

Master of Science in Forensic Science Directed Research Projects

Although temperature has been identified as the most important catalyst affecting blow fly larval development, other variables encountered by these species, such as the fat and moisture content of the food source, may have an impact on accurate estimations of the PMImin in forensic investigations. Thus, body composition differences between human carrion, such as variations in body fat and water percentages between younger and older individuals, or heavier persons, could affect blow fly developmental rates, and in turn cause over or under estimations of the PMImin. In this study, the weight, length, and development times of Lucilia sericata third instar …

A Comparative Analysis Of Swabbing Solutions For Sexual Assault Evidence: Direct Impact On Spermatozoa And Dna Recovery, Olivia Q. Kassie

Master of Science in Forensic Science Directed Research Projects

Forensic laboratories struggle with a backlog of sexual assault cases, prompting the need for effective techniques to develop consistent protocols and address scientific gaps. Challenges in recovering optimal DNA from assault evidence stem from factors like collection timing and sampling variables. Variations in lab protocols can lead to differences in DNA yields and profile success. This was observed in a state lab when protocols were developed allowing the use of acetone to swab sexual assault evidence. The use of acetone originated due to their case-routing protocols in multi-section cases involving latent fingerprints. While not necessary for sexual assault and porous …

Cellular Autofluorescence Signatures For Determining Time Since Deposition Of Whole Blood, Hannah Lamer

Master of Science in Forensic Science Directed Research Projects

Knowledge of the relative window of time that evidentiary biological material was deposited at a crime scene, referred to as the time since deposition (TSD), would help investigators determine which evidentiary items are most probative and assist with scene reconstruction. One commonly encountered body fluid at a crime scene is blood. Various methods for estimating TSD of bloodstains have been developed, but none to date have been validated for use in the forensic laboratory. Recently, flow cytometry measurements of cellular autofluorescence and size/structure have been investigated as a signature for TSD of saliva and touch epidermal cells. This study assessed …

Using The Microbiome To Assess The Health Status Of The Individuals Recovered From The East Marshall Street Well, Arian Karim

Master of Science in Forensic Science Directed Research Projects

In 1994, during the construction of the Kontos Building on the Medical College of Virginia Campus, a well on East Marshall Street was discovered to contain artifacts and human remains of African descent. Upon archaeological analysis by the Smithsonian, the ancestral remains can be dated back to the mid-19th century, when grave robbing and the use of cadavers for anatomical purposes [BS1] in medical schools were common. The human oral microbiome is the second largest human microbiota, containing over 600 species of organisms. Dysbiosis of the oral microbiome can lead to the overgrowth of pathogenic species, resulting in illness and …

Assessing The Effectiveness Of Alternative Light Sources In The Search For Skeletal Remains, Oiivia L. Thompson

Master of Science in Forensic Science Directed Research Projects

Many search and recovery operations for human skeletal remains are unsuccessful due to difficulties recognizing bones in outdoor environments even when evidence indicates the last known whereabouts of a missing individual. Though the collagen component of bone is known to emit fluorescent, this property has not been leveraged consistently during skeletal remains searches. Thirty-six mock searches were completed in 5000 ft² zones of eastern deciduous forest by volunteers associated with the Virginia Department of Emergency Management. Pig and deer bones were scattered and partially concealed under brush and leaf cover. Pairs of volunteers were allowed up to one hour …

Opioid System's Potential Involvement In Ketamine's Cognitive And Antidepressant-Like Effects In Rats, Fan Zhang

Theses and Dissertations

Depression stands as one of the most pervasive mental health challenges worldwide, including the United States. Conventional antidepressants typically display a delayed onset of therapeutic action (4-8 weeks) and lack efficacy in a substantial portion of patients (over 40% of depressed patients are treatment-resistant). The dissociative anesthetic ketamine, which is a glutamatergic N-methyl-D-aspartate (NMDA) antagonist, has emerged as a promising novel treatment due to its rapid and prolonged antidepressant effects, and it is especially beneficial for treatment-resistant depression. Nevertheless, the precise neurochemical mechanisms underlying ketamine’s antidepressant properties remain elusive, hampering the development of safer and more efficacious treatments. The present …

The Organophosphate Malathion Underlies Locomotor Performance Changes And Motoneuron Morphological Alterations In Sprague Dawley Rats, Kaitlyn Marie Miller

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Organophosphates are known neurotoxicants; however, they remain in use as flame retardants and are heavily used as pesticides. Malathion is an organophosphate insecticide commonly used in the United States since 1956. Despite known neurobehavioral impacts, there is little data available as to how repeated, occupational like, malathion exposures impact locomotion and the structure of motoneurons. We hypothesized that low-level repeated exposure to malathion would result in motor function deficits with anatomical alterations in the alpha-motoneurons (MN) consistent with neurodegeneration. Also, we hypothesized that galantamine would be neuroprotective against the effects of malathion. To test our hypothesis, we exposed male and …

Investigating Calcium Levels During Excitation-Contraction Coupling In Model Huntington's Disease Skeletal Muscle, Pooneh Hajmirza Mohammadi Kamalabadi

Browse all Theses and Dissertations

One of the less studied aspects of Huntington’s disease (HD) is its impact on skeletal muscle function. Recent studies have suggested that HD patients exhibit altered muscle function and reduced muscle strength, which may be related to defects in Ca2+ handling during excitation-contraction coupling (ECC). However, the underlying mechanisms of these alterations remain poorly understood. This study aims to determine if the myoplasmic Ca2+ during ECC is reduced in late-stage R6/2 (mouse model of HD) muscle fibers compared to age-matched controls during twitch or tetanic stimulation. Such a decrease would help explain weakness or fatigue in the model HD muscle. …

Role Of Interleukin 2 Receptor Alpha In The Cellular Response To Dna Damage, Shristi Khanal

Browse all Theses and Dissertations

The interleukin 2 receptor alpha (IL-2Rα), which is integral to the functioning of the cytokine interleukin 2, has recently been found to be present in different cell types besides just lymphocytes. Previous studies done by our laboratory showed that several other non-lymphoid cells including smooth muscle cells express IL-2Rα. Vascular smooth muscle cell (VSMC) deficient in IL-2Rα exhibited increased proliferation, decreased size, and hypodiploid DNA content when compared to wild type (WT) cells. Previous studies also suggested that VSMC proliferate in response to IL-2 and that IL-2 increases following interventions to stimulate intimal hyperplasia in vivo. These findings, in total, …

Novel Approaches To Treatment Of Hyperexcitability In Skeletal Muscle, Phillip Vance Walker Ii

Browse all Theses and Dissertations

Myotonia Congenita (MC) is a rare, inherited ion channelopathy caused by a loss-of-function mutation in the CLCN1 gene. The resulting downregulation of the skeletal muscle chloride channel (ClC-1) results in hyperexcitable skeletal muscle fibers that fire action potentials involuntarily. Patients with MC suffer from debilitating stiffness due to myotonia, described clinically as delayed muscle relaxation following voluntary contraction. Skeletal muscle is a unique system we use in this study to advance our understanding of the pathophysiology underlying MC, and other channelopathies characterized by hyperexcitable cells (i.e., forms of epilepsy and cardiac arrhythmia). Furthermore, re-assessing what makes anti-myotonic drugs such as …

A Novel In-Vitro Approach To Investigate The Molecular Interaction Between Inhaled Particles And The Human Pulmonary Surfactant System, Bryan Mayville

Browse all Theses and Dissertations

The deployment of military personnel to austere environments poses significant pulmonary health risks from inhalation of particulates including sand, dust, pollution, and heavy machinery exhaust. When particles are less than 10 µm in diameter, they can penetrate deep within the alveoli and embed within the protective lung surfactant monolayer. Here, absorption of surfactant lipids to the particle surface disrupts the essential monolayer configuration needed to reduce surface tension and prevent atelectasis, thus leading to disease. Currently, there are no in vitro surfactant-producing lung cell models capable of studying the effects of inhaled particles on the human pulmonary surfactant system. A549 …

Insights Into Hyperkalemic Periodic Paralysis: Novel Mechanisms And Treatments, Christopher Dupont

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Hyperkalemic Periodic Paralysis (hyperKPP) is an inherited channelopathy that leads to incapacitating episodes marked by severe skeletal muscle weakness or total paralysis, often accompanied by muscular stiffness (myotonia). The periods of muscle dysfunction are thought to be caused by the elevation in extracellular potassium (K). This autosomal dominant disorder is associated with mutations in the skeletal muscle voltage-gated sodium channel (Nav1.4). A multitude of inciting factors have been documented, including rest post-exertion, a potassium-rich diet, exposure to cold temperatures, psychological stressors, and fatigue. Given the variability of the triggers and the debilitating manifestation of symptoms, individuals afflicted by hyperKPP have …

Exploring The Effects Of Choline Treatment On Dystrophic Phenotype, Brooklyn Morris

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Duchenne Muscular Dystrophy (DMD) is a devastating progressive muscular disorder caused by a mutation in the dystrophin gene affecting approximately 1 in 3,500 males. However, despite its prevalence, there is currently no cure for this disease. Mutations in dystrophin lead to enhanced inflammation, fibrosis, cell death, development, and decreased skeletal muscle function. Phosphatidylcholine, synthesized by choline, is a major phospholipid that functions in maintaining and synthesizing cell membranes and has previously been found to be decreased in DMD patients. This led us to hypothesize that the dystrophic phenotype could be improved through treatment with choline. In this study, we used …

Comparative Analysis Of Motoneuronal C-Bouton Structural Alterations In Als And Aging, Shelby Nicole Ward

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Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease characterized by motor neuron (MN) death resulting in paralysis and eventually death. ALS has greater prevalence in older populations sharing characteristics with aging like muscle weakness and MN type specific degeneration. MNs innervate skeletal muscles and control muscle contraction through their excitability which is altered in both conditions. C-Boutons are a cholinergic, excitatory synaptic input to MNs and have been studied in ALS and aging but have produced inconsistent findings and undesired gaps. We used immunohistochemistry to label mouse lumbar spinal cord and separate MN types. 60x imaging and automated analysis was …

Studies Of Selective And Reversible Inhibitors Of Calcium Entry In Immune Cells, Naga Lakshmi Gandi

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Calcium signaling is a crucial regulator in many cellular processes, from immune response modulation to gene expression and apoptosis. Here, I have investigated the dynamics of calcium entry pathways, namely, store-operated calcium entry and store-independent calcium entry using a variety of cell types such as Jurkat T cells, HEK-293 cells and murine macrophages. Using fluorescence calcium imaging and selective inhibitor compounds, I have investigated the contributions of Orai and related calcium channels to calcium entry in both immune and non-immune cell types. The results demonstrate the pharmacological modulation of store-operated and store-independent calcium entry, showing the ability of compounds such …

Sex-Driven Alterations In Aging Alpha Motoneurons: Exploring Size, Type, Density, And Kv2.1 Channel Expression, Kalin R. Gerber

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This research explores how motor neurons (MNs) and Kv2.1 clustering change with age, emphasizing sex differences and MN subtypes. We found that MN density decreases with age in both sexes, while soma size increases in male mice. FF MNs were the most affected, and old weak mice had smaller MNs than their stronger counterparts, underscoring FF MN vulnerability. Baseline studies revealed that FF and FI MNs have larger Kv2.1 clusters compared to FR and S MNs. Female mice had smaller, denser Kv2.1 clusters than male mice, suggesting less clustering in females. With age, Kv2.1 clusters grew larger but became less …

Investigating The Effects Of Human Astroviruses On Aquaporins And Tight Junction Proteins In Vitro, Makalia T. Robinson

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Human Astroviruses (HAstV) are positive sense non-enveloped single stranded RNA viruses. HAstV is divided into classical (serotype 1-8) and non-classical (HAstVMLB 1-3 and HAstV-VA1-5) astroviruses. Classical HAstV is known to cause diarrhea in children, elderly, and immunocompromised patients. Additionally, the etiology of nonclassical HAstV remains unknown. However, recent studies have implicated non-classical HAstV closely associated with immunocompromised patients diagnosed neurological diseases like acute encephalitis. The mechanisms by which HAstV cause diarrhea is currently unknown. Aquaporins (AQPs) are integral membrane proteins that regulate the movement of water and small uncharged molecules across the cell membrane in response to osmotic gradients. Tight …

The Price Of Criminal Law, Russell M. Gold

Articles

Should tax dollars pay for more criminal law, better public schools, or a new community center? Different counties will answer the question differently, but facing these tradeoffs is profoundly important to democratic governance. Nonetheless, because the criminal legal system diffuses power and hides and offioads costs, officials and voters do not have to honestly consider that question. These structural features place a hidden thumb on the scale that gives counties more criminal enforcement than they pay for. That is a problem. Too much enforcement is particularly pernicious in criminal law: Incarceration inflicts tremendous suffering, especially in poor communities of color. …

Transgender Students And The First Amendment, Dara Purvis

Faculty Scholarship

Suppose a transgender child experiences teasing and harassment from their classmates, whose hostile reactions interrupt the school day. School administrators tell the transgender child that, in order to allow educational activities to continue, they must dress in more gender-neutral clothing, ideally cionsistent with the sex they were assigned at birth. The student's parents protest, arguing that their child's clothing is speech that expresses their gender identity. The school points to Tinker v. Des Moines, allowing suppression of student speech where it creates a material disruption, as well as recent legislation characterizing discussion of gender identity as lewd and obscene. …

The Nebulous Binaries Of C.S. Lewis' Till We Have Faces, Amantia Menalla

Honors Undergraduate Theses

Much of C.S. Lewis’ novel Till We Have Faces can be examined through one of two binaries. The tension between rationality, or reason, and spirituality, or religion, is one of the core facets of both the text and its main character, Orual. Orual feels the pull of both ideas and thus spends most of the novel in a nebulous space between them. This tension is further complicated by the space between the feminine and the masculine which she also inhabits. By covering her face and body, Orual de-emphasizes her feminine identity and takes on a masculine role, dressing as a …

Msw Portfolio, Courtney R. Nucito

Graduate Student Portfolios, Professional Papers, and Capstone Projects

This portfolio encapsulates my transformative journey through the realm of social work, detailing experiences, reflections, and insights gained throughout my academic and professional endeavors. Rooted in a commitment to social justice and ethical practice, my narrative unfolds through various roles and settings, each contributing to my growth as a compassionate and effective social work practitioner.

Addressing The Occupational Needs Of College Students, Amanda Lam, Ashley Keates, Marissa Heirich, Noreen Pervaiz, Albert Andrew Mendoza

Occupational Therapy | MSOT Capstone Projects

The Dominican Occupational Therapy (OT) Coaching project was led by a team of five master's level OT students in collaboration with the Integrative Coaching (IC) team at Dominican University of California. Aligned with the Occupational Therapy Practice Framework 4th edition (2020), the project supported various aspects of student wellness, such as academics, healthy lifestyles, stress management, sleep hygiene, and time management. In the fall of 2022, the team conducted a comprehensive literature review and needs assessment of Dominican's Student Success Center (SSC), actively engaged in SSC and IC meetings, and delivered an educational presentation on OT to SSC staff. The …

Intergenerational Connections: Us College Students’ Attitudes And Expectations Toward Older Adults And Aging In An Online Critical Community Engagement Project, Sandra L. Faulkner, Madison Pollino, Jaclyn Shetterly, Wendy K. Watson

Journal of Communication Pedagogy

We present a critically focused, semester-long community engagement project entitled Intergenerational Connections as a form of critical interpersonal and family communication pedagogy (CIFCP). The project utilized Zoom to connect small groups of undergraduate students in the US enrolled in a course on relational communication with older adults in a long-term care facility to dialogue about close relationships. We were interested in how dialogue across generations maintained or altered students’ attitudes and expectations about communicating with older adults and analyzed student reflections before, during, and after the dialogues using a turning point analysis. The study was framed using Social Identity Theory …

Development Of Novel Hydrogel Materials For Viscoelastically Accurate Brain Phantoms, Lila G. Schandler

Theses and Dissertations

Current methods such as concussion tests and Magnetic Resonance Imaging (MRI) scans only reveal structural damage post-injury. Creating an anatomically accurate model of the brain that mimics brain structure and viscoelastic properties will further allow medical professionals to analyze concussion events and create curated and personalized plans of recovery for a patient’s unique brain injury.

Values have been established for the necessary viscoelastic properties (time constants and elasticity) to mimic the native human brain tissue such as grey matter and white matter. The mechanical properties of the hydrogel are tunable by varying the concentration of Phytagel (PHY), a protein binding …

Parent Motivations For Enrolling Their Young Children In Early Childhood Programs: An Application Of The Theory Of Planned Behaviour, Rachel A. Mcgovern, Camilla Hodge, Edward Ruddell

Faculty Publications

This study used the theory of planned behaviour to identify and determine the relative influence of factors influencing parents’ decisions to enrol their young children (3 to 5 years old) in early childhood programs at a specific museum venue. An eliciting questionnaire was distributed to museum members with at least one child 3–5 years old to elicit outcome expectancies, normative beliefs, and control beliefs, and these responses were developed into items for the main questionnaire. Regression and correlation analyses were conducted to determine factors associated with parents’ intention to enrol their children. Parents across socioeconomic and educational backgrounds indicated they …

Confronting The Wildfire Crisis: Understanding Equity And Decision-Making In Wildfire Management Strategies, Katherine Huerta Sanchez

Dissertations, Master's Theses and Master's Reports

This study investigates how fuel managers incorporate equity and social vulnerability considerations into wildfire fuel reduction treatments on National Forest land and explores the resulting impact on the implementation of wildfire risk reduction policies. Through interviews, managers revealed differing perspectives on prioritizing social vulnerability characteristics and challenges such as insufficient information and resources to inform equitable fuel treatment decisions. These findings illuminate the complexities of integrating considerations of social vulnerability into decision-making processes for wildfire management at the wildland-urban interface, suggesting potential divergence from nationally supported policies aimed at equitably implementing wildfire risk reduction measures. The study findings underscore the …

Restoration Is Repairing Relationships: Bridging Indigenous And Western Sciences To Assess The Socio-Ecological Restoration Of Wild Rice (Zizania Palustris) On Keweenaw Bay Indian Community Homelands, Cassandra M. Reed-Vandam

Dissertations, Master's Theses and Master's Reports

Manoomin (wild rice, Zizania palustris) is an ecologically and culturally significant plant relative for the Keweenaw Bay Indian Community (KBIC). Historically, manoomin was present across much of the Great Lakes, however, their presence has declined since the early 1900s and, by 1990, virtually disappeared within KBIC homelands. Employing ethnography, surveys, focus groups, and conversations with Ojibwa knowledge holders, this research defined successful manoomin restoration for KBIC and developed socio-ecological attributes and indicators to assess restoration presented in the Medicine Wheel Framework for Manoomin Restoration. Surface water, sediment, and pore water samples at six different manoomin sites were analyzed to …

Exploring Usability In Exercise Interventions: From Conceptualization To Measurement And Application, Anne Inger Mørtvedt

Dissertations, Master's Theses and Master's Reports

Exercise interventions hold promise for preventing and treating numerous conditions, diseases, and injuries. However, these interventions will only be effective if they are being used. Unfortunately, uptake and adherence to prescribed exercise and physical activity guidelines are insufficient. Some reasons for this include lack of knowledge, resources, flexibility, and enjoyment. Exercise program developers need to not only consider the effectiveness of the program during the development phase, but also involve end-users and receive feedback on program usability to determine likelihood of uptake and adoption. Usability testing can be used to detect barriers to use and implementation likelihood but has not …

Gis-Based Landscape Prioritization To Guide Invasive Species Survey, Erin R. Mauk

Dissertations, Master's Theses and Master's Reports

Utilizing prioritization models gives invasive species managers the capability to systematically target surveying and management toward priority areas, therefore maximizing limited financial resources by increasing the value that management can have on the landscape. In this report, Geographic Information System (GIS) techniques were utilized to produce an invasion risk model and a quality land model. Combining these two models produced a landscape-level prioritization model, which denoted the land quality and associated invasion risk across the study area. High-quality land was commonly distributed within Isle Royale National Park, along Lake Superior’s coastline, and within coastal wetlands and riparian habitats. Areas of …