Digital Commons Network^™

Dlk-Dependent Axonal Mitochondrial Fission Drives Degeneration After Axotomy, Jorge Gómez-Deza, Matthew Nebiyou, Mor R Alkaslasi, Francisco M Nadal-Nicolás, Preethi Somasundaram, Anastasia L Slavutsky, Wei Li, Michael E Ward, Trent A Watkins, Claire E Le Pichon

Faculty, Staff and Students Publications

Currently there are no effective treatments for an array of neurodegenerative disorders to a large part because cell-based models fail to recapitulate disease. Here we develop a reproducible human iPSC-based model where laser axotomy causes retrograde axon degeneration leading to neuronal cell death. Time-lapse confocal imaging revealed that damage triggers an apoptotic wave of mitochondrial fission proceeding from the site of injury to the soma. We demonstrate that this apoptotic wave is locally initiated in the axon by dual leucine zipper kinase (DLK). We find that mitochondrial fission and resultant cell death are entirely dependent on phosphorylation of dynamin related …

Age-Dependent Regulation Of Axoglial Interactions And Behavior By Oligodendrocyte Ankyring, Xiaoyun Ding, Yu Wu, Anna Vainshtein, Victoria Rodriguez, Emily Ricco, James T Okoh, Yanhong Liu, Daniel C Kraushaar, Elior Peles, Matthew N Rasband

Faculty, Staff and Students Publications

The bipolar disorder (BD) risk gene ANK3 encodes the scaffolding protein AnkyrinG (AnkG). In neurons, AnkG regulates polarity and ion channel clustering at axon initial segments and nodes of Ranvier. Disruption of neuronal AnkG causes BD-like phenotypes in mice. During development, AnkG is also expressed at comparable levels in oligodendrocytes and facilitates the efficient assembly of paranodal junctions. However, the physiological roles of glial AnkG in the mature nervous system, and its contributions to BD-like phenotypes, remain unexplored. Here, we show that oligodendroglia-specific AnkG conditional knockout results in destabilization of axoglial interactions in aged but not young adult mice. In …

Fentanyl, Narco-Terrorism, And National Security Politics, J. Richard Broughton

San Diego Law Review

This Article examines the use of a counterterrorism model to attack the fentanyl crisis. Rather than advocate for or against such a model, this Article envisions what employing a counterterrorism model against international drug traffickers would look like. Noting the dimensions of national security politics that intersect with counter-fentanyl policy, this Article will evaluate three distinct counterterrorism approaches: (1) using criminal counterterrorism statutes in federal indictments and prosecutions; (2) designating drug trafficking organizations as Foreign Terrorist Organizations (FTOs); and (3) authorizing the use of military force against Mexican cartels. While the current state of bellicose national security politics can inform …

The Uses Of The Border, Salvador G. Sarmiento

San Diego Law Review

It is a good and necessary time to discuss “American Law at the Border” to better understand and engage with the politics and laws ostensibly addressing U.S. borders. Let’s hope it is also an exercise in understanding the borders of U.S. law itself—the limits, the periphery, and the jagged edges. A discussion on American law at the border should make clear that what is considered at the margins—starting with migrant labor—is central to U.S. economic, legal, and political life.

The first section of this Article will discuss the political uses of the border, beginning with the most recent example of …

Impact Of Chia Seed Ingestion And Physical Activity On Insulin Resistance And Triglyceride Levels In Men And Women, Ali Abdulraheem Jabbar, Maysaa Jalal Majeed, Muataz Fawzi Hussein

Modern Sport

The Chia seeds are a herbaceous plant native to southern Mexico and Guatemala. They have many nutritional and medicinal uses because they contain fibers, minerals and vitamins. These components have a significant impact on insulin resistance, which is considered a source of risk for many diseases, most notably type 2 diabetes. High levels of triglycerides. It is considered a source of danger for many diseases, the most important of which is heart disease. This study aims to evaluate the effect of consuming chia seeds on parameters of insulin resistance and triglyceride levels in individuals who suffer from insulin resistance. The …

Refereed Research: A Sufficientarian Approach To Consumption Ethics In The Face Of Climate Change, Srinivas Venugopal, Ronika Chakrabarti

Subsistence Marketplaces

In this paper, we adopt a sufficientarian ethics approach to study the concern of consumption inadequacy engendered by climate change in subsistence communities. The sufficientarian approach to consumption ethics dictates that in the face of climate change, priority should be afforded to establishing and maintaining consumption adequacy in low-income settings. We elaborate on the basic principles underlying sufficientarian ethics and compare it to alternative approaches such as utilitarian ethics and prioritarian ethics. Subsequently, we turn our attention toward specifying how poverty and the deleterious impact of climate change combine to render subsistence communities more vulnerable to consumption inadequacies as compared …

“Strange And Unusual Things”: Teaching The Speculative Fiction Of Margaret Cavendish, E Mariah Spencer

ABO: Interactive Journal for Women in the Arts, 1640–1830

Margaret Cavendish, a prolific author and natural philosopher, defies categorization with her multifaceted contributions to literature and philosophy. While she is recognized for her groundbreaking work in various academic fields, her literature also resonates within the realms of science fiction, weird fiction, and speculative genres. This essay explores innovative approaches to teaching Cavendish's work, particularly within the realm of speculative literature, using an interdisciplinary lens. Drawing on perspectives including monster theory and the concept of the abject, students are prompted to critically engage with Cavendish's texts. By examining themes such as the monstrous, the supernatural, and the occult, students uncover …

Teaching Margaret Cavendish Through The Lens Of Scientific Anti-Realism, Kevin Lower

ABO: Interactive Journal for Women in the Arts, 1640–1830

Margaret Cavendish (1623-1673) only began receiving sustained attention by historians of philosophy in recent decades, but she is being rapidly integrated into the early modern philosophical canon. This essay explores one dimension of teaching Cavendish that presents difficulties for introductory philosophy students: her criticisms of experimental philosophy. Section one explains how I integrate Cavendish into the narrative of my course, identifying both historical and thematic strategies for including her in introductory philosophy courses. Section two describes methods for teaching Cavendish’s natural philosophy and identifies a pedagogical challenge to presenting her criticisms of experimental philosophy. In their first exposure to Cavendish, …

An Argumental Discourse Before All: An Approach To Teaching Cavendish, Jonathan L. Shaheen

ABO: Interactive Journal for Women in the Arts, 1640–1830

Cavendish directs readers of Observations upon Experimental Philosophy to begin engaging her work through "An Argumental Discourse." In this piece, I describe a way of thinking about the structure of that piece and how to understand two of its major moments in relationship to standardly taught material. The first moment is the dilemma about how inanimate matter is moved, with its response centered on treating a horse and its rider as a paradigm example of causation. The article shows how the argument involves Cavendish's conceptions of causation, minds, bodies, and mind-body interaction. The second moment has to do with the …

Jumping Pens And Historical Vertigo: Teaching Cavendish In The Twenty-First Century, Anne M. Thell

ABO: Interactive Journal for Women in the Arts, 1640–1830

"Jumping Pens" considers "historical and epistemological vertigo" as a useful pedagogical rubric for teaching the works of Margaret Cavendish (1623-73) in contemporary university classrooms. Specifically, it looks back across more than a decade of teaching Cavendish in diverse settings in order to show how exciting this author can be for students who are just starting out in the discipline of literary studies. As this essay argues, Cavendish can make us all better, more self-aware instructors, while also developing critical and historical acumen in our students.

Enfilade And Variations: Interpreting Salon Music Through Residential Architecture In Eighteenth-Century Paris, Nicole Vilkner

ABO: Interactive Journal for Women in the Arts, 1640–1830

In eighteenth-century Parisian salons, theme and variations on popular melodies proliferated. Elaine Sisman’s seminal study of variation form provides a foundational approach for understanding variations as rhetorical exercises that persuade through differences in syntax and expression. In this vein, scholars have comprehensively affirmed the ways that salon music reflects the dialogic and sociable practices of Enlightenment receptions (Hanning, Sutcliffe, and Zohn). I suggest that theme and variation form is not only connected to the rhetoric of the salon, but that the concept of sequential elaboration is a principle that was also prized in residential architecture.

Drawing from theoretical writings of …

Simulating An Eighteenth-Century Enlightenment Salon: Embodied Experience And Scholarly Investigation, Jennifer M. Jones

ABO: Interactive Journal for Women in the Arts, 1640–1830

This paper reflects on my experience simulating an 18th-century French salon for an academic conference and in an undergraduate classroom. I argue that simulating a salon is heuristically as well as pedagogically useful and allows historians to test hypotheses about the nature of the salon and the role of the salonnière proposed by scholars such as Dena Goodman and Antoine Lilti. The essay also engages with some of the important insights gained from feminist and performance theory regarding historical reenactment. Thus I caution against naive attempts to reenact the past and instead suggest practices that foster open-ended conversations about the …

Introduction: The Salon And The Senses In Eighteenth-Century France, Jennifer M. Jones, Rebecca Cypess

ABO: Interactive Journal for Women in the Arts, 1640–1830

An introduction to a special collection of essays exploring the salon and the senses in eighteenth-century France. Provides an overview of the critical historiography on the salon and the role of salon hostesses. Explores new approaches to salon within the context of the history of the senses, with attention to the role of art, music, and sociability in salon culture. Provides an overview of the essays in the collection.

What Is It Like To Be A Cognitive Ethologist?, Anne Wordsworth She/Her

Animal Sentience

Donald Griffin not only made the study of animal minds acceptable for scientific investigation but the work of the cognitive ethologists that he inspired made animals and their lives fascinating and accessible to the greater public. The scrupulously detailed examination of his life and work by Ristau ensures that this history will always be there to respect his work and his legacy

Leading Towards The Queerest Insurrection: Queer Anarchism And Leadership Studies, Josie Holland

Interdisciplinary Journal of Leadership Studies

Leadership shapes our world, our narratives, and what is possible to imagine. Unfortunately, leadership studies has some trouble imagining queer leadership, or rather, queer theory and leadership studies. In order to integrate the more abstract aspects of queer theory with the largely evidence-based field of leadership studies, I draw on the texts, history, practices, and theory of queer anarchism. This article aims to highlight a gap in leadership studies as it currently stands, and through accounting for the absence of queer anarchism in leadership studies, expand what leadership looks like. To this end, I suggest the framing of ‘leadership acts’ …

Utilitarian And Self-Representational: Player-To-Player Character Relationships In Final Fantasy Xiv, Zu Er Guan

Theses

This thesis studies the relationships between video game players and their player characters, the characters that they control, through the lens of player purpose and intent by conducting research using an online survey developed and analyzed using an interdisciplinary methodology based on existing studies in the field regarding avatar identification, player psychology, and queer and feminist theory. This thesis posits that are two types of player relationships with their player characters based on player intent—player characters as the personal avatar and representation of the self, and player characters as a tool for the execution of player control and gameplay experience. …

Exploring Predictors Of Purchase Intention Toward Plant-Based Meat, Kristiana Ginelle D. Monares, Ken Sean D. Roque, Melissa A. Santos, Sofia Marianne S. So, Catherine A. Valentin, Alvin Patrick M. Valentin

Journal of Management for Global Sustainability

Plant-based meat (PBM) presents itself as a sustainable alternative to meat production systems that have adversely impacted consumers’ health and the environment at large. In the Philippine context, however, there are only a few PBM manufacturers to date even though production has been emerging with expanded options for consumers to consider. Moreover, while PBM’s potential allows it to be a prospective working food system in the country through the promotion of healthier food chains, the literature on PBM in the Philippines is scant. This study thus contributes to this literature by exploring predictors of purchase intention toward PBM. The researchers …

Comparison Of Three Chimeric Zika Vaccine Prototypes Developed On The Genetic Background Of The Clinically Proven Live-Attenuated Japanese Encephalitis Vaccine Sa14-14-2, Byung-Hak Song, Jordan C. Frank, Sang-Im Yun, Justin G. Julander, Jeffrey B. Mason, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Xin Dai, Young-Min Lee

Animal, Dairy, and Veterinary Science Student Research

Zika virus (ZIKV) is a medically important mosquito-borne orthoflavivirus, but no vaccines are currently available to prevent ZIKV-associated disease. In this study, we compared three recombinant chimeric viruses developed as candidate vaccine prototypes (rJEV/ZIKV^MR-766, rJEV/ZIKV^P6-740, and rJEV/ZIKV^PRVABC-59), in which the two neutralizing antibody-inducing prM and E genes from each of three genetically distinct ZIKV strains were used to replace the corresponding genes of the clinically proven live-attenuated Japanese encephalitis virus vaccine SA₁₄-14-2 (rJEV). In WHO-certified Vero cells (a cell line suitable for vaccine production), rJEV/ZIKV^P6-740 exhibited the slowest viral growth, formed …

Vaccines Targeting P53 Mutants Elicit Anti-Tumor Immunity, Dafei Chai, Xu Wang, Chunmei Fan, Junhao Wang, Jing Ming Lim, Xinfang Yu, Ken H Young, Yong Li

Faculty, Staff and Students Publications

The p53 tumor suppressor is commonly mutated in cancer; however, there are no effective treatments targeting p53 mutants. A DNA vaccine gWIZ-S237G targeting the p53 S237G mutant, which is highly expressed in A20 murine tumor cells, was developed and administered intramuscularly via electroporation, either alone or in combination with PD1 blockade. The anti-p53-S237G immunization elicited a robust protective response against subcutaneous A20 tumors and facilitated the infiltration of immune cells including CD8+ T cells, NK cells, and DCs. The vaccine enhanced the induction and maturation of CD11c+, CD103+CD11c+, and CD8+CD11c+ cells, which in turn promoted tumor-specific antibody production, as well …

Chiropterophagy By The Siberian Weasel (Mustela Sibirica): The First Record In The Russian Far East, Valeriya E. Omelko, Mikhail P. Tiunov

International Journal of Speleology

Chiropterophagy is a widespread phenomenon, but only one case of bat consumption by sable has been documented in the Russian Far East. This study presents the first recorded instance of chiropterophagy by the Siberian weasel (Mustela sibirica) in the southern Russian Far East, based on excrement collected from the Solyanik Cave in the southern Sikhote-Alin Mountains in 2007. The excrement included bones from three bat species (Murina hilgendorfi, Myotis bombinus, Plecotus ognevi) and one vole species (Myodes rutilus). Our findings suggest that the Siberian weasel visits caves during the cold season, although …

Hauntings Of Publication Deaths, Possibilities For Our Academic Present, Kathleen (Kaye) A. Hare, Amber Moore

Journal of Feminist Scholarship

In this paper, we use a feminist dialogue to query publication deaths that haunt us. We ruminate on respective past publications that remain unread and uncited, despite encapsulating defining events in our development as scholars. For Author Kaye Hare this was an affective narrative that troubled a seemingly ideal moment of public scholarship; for Author Amber Moore, this was a hard-fought analysis of precarious intersectional feminist resistance in popular culture. Inspired by the horror-film genre “spectral incognizance,” we methodologically deploy a multivocal dialogical structure based on multiple revisitings that attenuate anxieties of metaphorical publication death, before revealing that this death …

Using Gis To Visualize Daily Routines At The Neighborhood And Household Scales: A Feminist Approach Using A Nineteenth-Century Diary, Sterling D. Quinn

Journal of Feminist Scholarship

Feminist scholars in the discipline of geography have noted the ways that routine activities at the household and neighborhood scales contribute to power relations and identity formation. Geographic information systems (GIS), with their ability to create multiple visual representations of people’s experiences in space at any scale, hold great potential for revealing these local processes and patterns that are often lost or invisible in broader historical narratives, especially when they pertain to women and children. This article contributes an example by using GIS to encode and map everyday events in a diary kept by Elisabeth Koren, a nineteenth-century Norwegian immigrant …

Folic Acid And Methyltetrahydrofolate Supplementation In The, Karen E Christensen, Marie-Lou Faquette, Daniel Leclerc, Vafa Keser, Yan Luan, Jeanna L Bennett-Firmin, Olga V Malysheva, Alaina M Reagan, Gareth R Howell, Marie A Caudill, Teodoro Bottiglieri, Rima Rozen

Faculty Research 2024

Background/Objectives: The MTHFR677C>T gene variant results in a thermolabile MTHFR enzyme associated with elevated plasma homocysteine in TT individuals. Health risks associated with the TT genotype may be modified by dietary and supplemental folate intake. Supplementation with methyltetrahydrofolate (methylTHF) may be preferable to folic acid because it is the MTHFR product, and does not require reduction by DHFR to enter one-carbon folate metabolism. In the Mthfr677C>T mouse model for this variant, female 677TT (TT) mice have an increased incidence of hepatic steatosis. The objective of this study was to compare the effects of methylTHF and folic acid supplementation …

On Resource Testing Of Straight-Bevel Gears In Drive Mechanisms Of Cotton Machines, Shoraxmat Asqarovich Shaabidov, Umid Mamazokir Ugli Mamasobirov, Azizbek G‘Ulom O‘G‘Li Xusanbayev

Technical science and innovation

This article is dedicated to endurance testing of straight-tooth bevel gear reducers used in various machines, including cotton farming machinery. The choice of the test object is due to the fact that many machines require bevel gear transmissions for spatial arrangement of components. These gears enable the transmission of rotational motion between intersecting shaft axes within compact external dimensions. Additionally, bevel gear transmissions are the most common type of gear mechanisms after cylindrical ones.

For several objective reasons, straight-tooth bevel gears have gained wide use. Unlike cylindrical gears, they are more sensitive to manufacturing and assembly errors as well as …

Development Of The Principles Of Forming A Database For Geoinformation Mapping Of Tourism (As An Example Of Bostanlyk District Of Tashkent Region), Lola Xoji-Akbarovna Gulyamova, Risolat Raximjon Qizi Nizomova

Technical science and innovation

When creating tourist assessment maps for the purpose of tourist mapping, special attention is paid to determining the criteria for the formation of a database. To assess tourism opportunities in terms of natural conditions, ecology and socio-economic factors, data collection, their characteristics and requirements for them were studied. In order to improve the quality of sources of tourist assessment maps, parables of working with the internet and real-time removable data, creating a map based on social network data were considered. Proposals were made to create, manage and maintain a big data base. The ArcGIS program analyzed the possibilities of describing …

Bayesian Semiparametric Inference In Longitudinal Metabolomics Data, Abhra Sarkar, Ornella Cominetti, Ivan Montoliu, Joanne Hosking, Jonathan Pinkney, Francois Pierre Martin, David B. Dunson

Peninsula Medical School

The article is motivated by an application to the EarlyBird cohort study aiming to explore how anthropometrics and clinical and metabolic processes are associated with obesity and glucose control during childhood. There is interest in inferring the relationship between dynamically changing and high-dimensional metabolites and a longitudinal response. Important aspects of the analysis include the selection of the important set of metabolites and the accommodation of missing data in both response and covariate values. With this motivation, we propose a flexible but parsimonious Bayesian semiparametric joint model for the outcome and the covariate generating processes, making novel use of nonparametric …

Parp Inhibition Radiosensitizes Brca1 Wildtype And Mutated Breast Cancer To Proton Therapy, Mariam Ben Kacem, Scott J Bright, Emma Moran, David B Flint, David K J Martinus, Broderick X Turner, Ilsa Qureshi, Rishab Kolachina, Mandira Manandhar, Poliana C Marinello, Simona F Shaitelman, Gabriel O Sawakuchi

Faculty, Staff and Student Publications

Aggressive breast cancers often fail or acquire resistance to radiotherapy. To develop new strategies to improve the outcome of aggressive breast cancer patients, we studied how PARP inhibition radiosensitizes breast cancer models to proton therapy, which is a radiotherapy modality that generates more DNA damage in the tumor than standard radiotherapy using photons. Two human BRCA1-mutated breast cancer cell lines and their isogenic BRCA1-recovered pairs were treated with a PARP inhibitor and irradiated with photons or protons. Protons (9.9 and 3.85 keV/µm) induced higher cell kill independent of BRCA1 status. PARP inhibition amplified the cell kill effect to both photons …

Extracorporeal Shock Wave Therapy For Children And Adolescents Diagnosed With Osgood Schlatter Disease To Reduce Knee Pain: Systematic Review, Mohammad Tamboosi, Mohammad Tamboosi, Hashim Alharthi, Abdulaziz Bukhari Bukhari, Abdulrahman Alsulami, Waleed Hawari, Yazeed Alahmadi, Rayan Alshahrani, Rayan Alshamrani

Journal of Medical Rehabilitation Science

Aim: The current systematic review aimed to investigate the effectiveness of ESWT on children and adolescents diagnosed with OSD to reduce their knee pain. Method: An electronic search for studies about ESWT on patients with OSD that were published in English in different databases (CINAHL, PubMed, MEDLINE, Scopus, and PEDro). National Heart, Lung, and Blood Institute’s Quality assessment tool was used to evaluate the included studies. Results: According to the search strategy, from 2807 articles, only 2 studies (retrospective cohort and RCT) were selected for the current review, with a total of 34 subjects (20 Males and 14 Females) diagnosed …

Cancer Organoids As Reliable Disease Models To Drive Clinical Development Of Novel Therapies, Giovanni Blandino, Ronit Satchi-Fainaro, Ingeborg Tinhofer, Giovanni Tonon, Sarah C Heilshorn, Yong-Jun Kwon, Ana Pestana, Carlotta Frascolla, Luca Pompili, Aurora Puce, Sara Iachettini, Annalisa Tocci, Sofia Karkampouna, Marianna Kruithof-De Julio, Piera Tocci, Nicla Porciello, Klizia Maccaroni, Daniela Rutigliano, Xiling Shen, Gennaro Ciliberto

Faculty, Staff and Student Publications

On September 23-24 (2024) the 6th Workshop IRE on Translational Oncology, titled "Cancer Organoids as Reliable Disease Models to Drive Clinical Development of Novel Therapies," took place at the IRCCS Regina Elena Cancer Institute in Rome. This prominent international conference focused on tumor organoids, bringing together leading experts from around the world.A central challenge in precision oncology is modeling the dynamic tumor ecosystem, which encompasses numerous elements that evolve spatially and temporally. Patient-derived 3D culture models, including organoids, explants, and engineered or bioprinted systems, have recently emerged as sophisticated tools capable of capturing the complexity and diversity of cancer cells …

Muramyl Peptide Blend Ameliorates Intestinal Inflammation And Barrier Integrity In Caco-2 Cells, Dmytro M. Masiuk, Victor S. Nedzvetsky, Giyasettin Baydas

Karbala International Journal of Modern Science

Intestinal barrier function depends on epithelial adhesion, which restricts permeability and microbial invasion from the internal environment. Impairment of barrier integrity and gut function is closely linked to pro-inflammatory changes. Inflammation is often the primary factor that provokes gut function disorders. The anti-inflammatory potential of postbiotics has been reported in recent years. Muramyl peptides (MPs) are small signaling molecules that stimulate intracellular pathogen receptors and can regulate cell responses. However, the molecular mechanisms of MPs' effects on intestinal cells remain unknown. The study of MPs treatment on lipopolysaccharide (LPS)-challenged Caco-2 intestinal cells aimed to investigate the postbiotic effects on intestinal …