Digital Commons Network^™

Programmable And Reversible Integrin-Mediated Cell Adhesion Reveals Hysteresis In Actin Kinetics That Alters Subsequent Mechanotransduction, Zheng Zhang, Guy M Genin, Et Al.

2020-Current year OA Pubs

Dynamically evolving adhesions between cells and extracellular matrix (ECM) transmit time-varying signals that control cytoskeletal dynamics and cell fate. Dynamic cell adhesion and ECM stiffness regulate cellular mechanosensing cooperatively, but it has not previously been possible to characterize their individual effects because of challenges with controlling these factors independently. Therefore, a DNA-driven molecular system is developed wherein the integrin-binding ligand RGD can be reversibly presented and removed to achieve cyclic cell attachment/detachment on substrates of defined stiffness. Using this culture system, it is discovered that cyclic adhesion accelerates F-actin kinetics and nuclear mechanosensing in human mesenchymal stem cells (hMSCs), with …

Conserved And Divergent Gene Regulatory Programs Of The Mammalian Neocortex, Nathan R Zemke, Daofeng Li, Xiaoyu Zhuo, Vincent Xu, Ting Wang, Et Al.

2020-Current year OA Pubs

Divergence of cis-regulatory elements drives species-specific traits

Multi-Ancestry Genome-Wide Association Study Of Cannabis Use Disorder Yields Insight Into Disease Biology And Public Health Implications, Daniel F Levey, Emma C Johnson, Arpana Agrawal, Et Al.

2020-Current year OA Pubs

As recreational use of cannabis is being decriminalized in many places and medical use widely sanctioned, there are growing concerns about increases in cannabis use disorder (CanUD), which is associated with numerous medical comorbidities. Here we performed a genome-wide association study of CanUD in the Million Veteran Program (MVP), followed by meta-analysis in 1,054,365 individuals (n

Multi-Ancestry Study Of The Genetics Of Problematic Alcohol Use In Over 1 Million Individuals, Hang Zhou, Emma C Johnson, Pamela A F Madden, Andrew C Heath, Arpana Agrawal, Et Al.

2020-Current year OA Pubs

Problematic alcohol use (PAU), a trait that combines alcohol use disorder and alcohol-related problems assessed with a questionnaire, is a leading cause of death and morbidity worldwide. Here we conducted a large cross-ancestry meta-analysis of PAU in 1,079,947 individuals (European, N = 903,147; African, N = 122,571; Latin American, N = 38,962; East Asian, N = 13,551; and South Asian, N = 1,716 ancestries). We observed a high degree of cross-ancestral similarity in the genetic architecture of PAU and identified 110 independent risk variants in within- and cross-ancestry analyses. Cross-ancestry fine mapping improved the identification of likely causal variants. Prioritizing …

Single-Cell Analysis Of Chromatin Accessibility In The Adult Mouse Brain, Songpeng Zu, Yang Eric Li, Et Al.

2020-Current year OA Pubs

Recent advances in single-cell technologies have led to the discovery of thousands of brain cell types; however, our understanding of the gene regulatory programs in these cell types is far from complete

Neurofibromin 1 Mutations Impair The Function Of Human Induced Pluripotent Stem Cell-Derived Microglia, Leonard D Kuhrt, Edyta Motta, Nirmeen Elmadany, Hannah Weidling, Raphaela Fritsche-Guenther, Ibrahim E Efe, Olivia Cobb, Jit Chatterjee, Lucy G Boggs, Marina Schnauß, Sebastian Diecke, Marcus Semtner, Corina Anastasaki, David H Gutmann, Helmut Kettenmann

2020-Current year OA Pubs

Neurofibromatosis type 1 (NF1) is an autosomal dominant condition caused by germline mutations in the neurofibromin 1 (NF1) gene. Children with NF1 are prone to the development of multiple nervous system abnormalities, including autism and brain tumors, which could reflect the effect of NF1 mutation on microglia function. Using heterozygous Nf1-mutant mice, we previously demonstrated that impaired purinergic signaling underlies deficits in microglia process extension and phagocytosis in situ. To determine whether these abnormalities are also observed in human microglia in the setting of NF1, we leveraged an engineered isogenic series of human induced pluripotent stem cells to generate human …

A Multisociety Delphi Consensus Statement On New Fatty Liver Disease Nomenclature, Mary E. Rinella, Samuel Klein, Et Al.

2020-Current year OA Pubs

The principal limitations of the terms NAFLD and NASH are the reliance on exclusionary confounder terms and the use of potentially stigmatising language. This study set out to determine if content experts and patient advocates were in favor of a change in nomenclature and/or definition. A modified Delphi process was led by three large pan-national liver associations. The consensus was defined a priori as a supermajority (67%) vote. An independent committee of experts external to the nomenclature process made the final recommendation on the acronym and its diagnostic criteria. A total of 236 panelists from 56 countries participated in 4 …

Apoe Genotype And Alzheimer Disease Risk Across Age, Sex, And Population Ancestry, Michael E Belloy, Shea J Andrews, Yann Le Guen, Michael Cuccaro, Lindsay A Farrer, Valerio Napolioni, Michael D Greicius

2020-Current year OA Pubs

IMPORTANCE: Apolipoprotein E (APOE)*2 and APOE*4 are, respectively, the strongest protective and risk-increasing, common genetic variants for late-onset Alzheimer disease (AD), making APOE status highly relevant toward clinical trial design and AD research broadly. The associations of APOE genotypes with AD are modulated by age, sex, race and ethnicity, and ancestry, but these associations remain unclear, particularly among racial and ethnic groups understudied in the AD and genetics research fields.

OBJECTIVE: To assess the stratified associations of APOE genotypes with AD risk across sex, age, race and ethnicity, and global population ancestry.

DESIGN, SETTING, PARTICIPANTS: This genetic association study included …

Identification Of New 4-(6-Oxopyridazin-1-Yl)Benzenesulfonamides As Multi-Target Anti-Inflammatory Agents Targeting Carbonic Anhydrase, Cox-2 And 5-Lox Enzymes: Synthesis, Biological Evaluations And Modelling Insights, Waleed A Badawi, Mahmoud Rashed, Alessio Nocentini, Alessandro Bonardi, Mohammad M Abd-Alhaseeb, Sara T Al-Rashood, Giri Babu Veerakanellore, Taghreed A Majrashi, Eslam B Elkaeed, Bahaa Elgendy, Paola Gratteri, Claudiu T Supuran, Wagdy M Eldehna, Mohamed Elagawany

2020-Current year OA Pubs

Multiple inhibitions of CA, COX-2 and 5-LOX enzymes has been recognised as a useful strategy for the development of anti-inflammatory drugs that can avoid the disadvantages of using NSAIDs alone. Here, we report new pyridazine-based sulphonamides (

What Can The Plasma Proteome Tell Us About Platelets And (Vice Versa)?, Steven Bruzek, Marisol Betensky, Jorge Di Paola, Thomas Diacovo, Neil Goldenberg, Vera Ignjatovic

2020-Current year OA Pubs

Multi-omics approaches are being used increasingly to study physiological and pathophysiologic processes. Proteomics specifically focuses on the study of proteins as functional elements and key contributors to, and markers of the phenotype, as well as targets for diagnostic and therapeutic approaches. Depending on the condition, the plasma proteome can mirror the platelet proteome, and hence play an important role in elucidating both physiologic and pathologic processes. In fact, both plasma and platelet protein signatures have been shown to be important in the setting of thrombosis-prone disease states such as atherosclerosis and cancer. Plasma and platelet proteomes are increasingly being studied …

Correlating Deep Learning-Based Automated Reference Kidney Histomorphometry With Patient Demographics And Creatinine, Nicholas Lucarelli, Brandon Ginley, Jarcy Zee, Sayat Mimar, Anindya S. Paul, Sanjay Jain, Seung Seok Han, Luis Rodrigues, Tezcan Ozrazgat-Baslanti, Michelle L. Wong, Girish Nadkarni, William L. Clapp, Kuang-Yu Jen, Pinaki Sarder

2020-Current year OA Pubs

KEY POINTS: The authors leverage the unique benefits of panoptic segmentation to perform the largest ever quantitation of reference kidney morphometry. Kidney features vary with age and sex; and glomeruli size may intricately link to creatinine, defying prior notions.

BACKGROUND: Reference histomorphometric data of healthy human kidneys are largely lacking because of laborious quantitation requirements. Correlating histomorphometric features with clinical parameters through machine learning approaches can provide valuable information about natural population variance. To this end, we leveraged deep learning (DL), computational image analysis, and feature analysis to associate the relationship of histomorphometry with patient age, sex, serum creatinine (SCr), …

Comprehensive Analysis Of The Effects Of Superplasticizer Variation On The Workability And Strength Of Ready-Mix Concrete, Tahsin Tareque, Fahmid Tousif, Mirza Addaito Billah, Abdullah Al Masum Jabir, Seyedali Mirmotalebi

Civil Engineering Faculty Publications

This experimental study aims to examine the influence of many crucial parameters on the workability and compressive strength of Ready-Mix Concrete (RMC). The study utilized two distinct varieties of superplasticizers obtained from the local market. The fine aggregates utilized in this study were sourced from Sylhet sand, whereas the coarse aggregates were comprised of boulder crushed stone chips. The experimental procedures adhered to the requirements outlined by ASTM. A comprehensive investigation was conducted on a range of concrete compositions that used diverse chemical admixtures. The slump test was performed at regular intervals of 15 minutes until the slump value reached …

Vehicle-Bridge Interaction Simulation And Damage Identification Of A Bridge Using Responses Measured In A Passing Vehicle By Empirical Mode Decomposition Method, Shohel Rana, Md. Rifat Zaman, Md. Ibrahim Islam Ifty, Seyedali Mirmotalebi, Tahsin Tareque

Civil Engineering Faculty Publications

To prevent early bridge failures, effective Structural Health Monitoring (SHM) is vital. Vibration-based damage assessment is a powerful tool in this regard, as it relies on changes in a structure’s dynamic characteristics as it degrades. By measuring the vibration response of a bridge due to passing vehicles, this approach can identify potential structural damage. This dissertation introduces a novel technique grounded in Vehicle-Bridge Interaction (VBI) to evaluate bridge health. It aims to detect damage by analyzing the response of passing vehicles, taking into account VBI. The theoretical foundation of this method begins with representing the bridge’s superstructure using a Finite …

Developing Recommendations For Broad Mite, Polyphagotarsonemus Latus Banks, In Blackberry, Rubus Subgenus Rubus, Jared Bradley Linn

Graduate Theses and Dissertations

Broad mite, (Polyphagotarsonemus latus Banks) (Acari: Tarsonemidae), is an economically important pest of blackberry production across the world. Since it was first reported on primocane-fruiting blackberries in 2010, little work has gone into developing IPM tools and understanding impact on floricane-fruiting cultivars. The objective of this thesis was to evaluate the effect of broad mite feeding on floricane-fruiting cultivars of blackberry, develop an efficient and accurate field scouting strategy, and investigate the impact of cultivar and trellising technique on the development of broad mite populations. Experiments consisted of evaluating floricane fruiting cultivars by quantifying broad mite populations and cataloging their …

Effect Of Cultural Strategies And Application Technologies On Weed Control In Flooded And Furrow-Irrigated Rice, Noah Hunter Reed

Graduate Theses and Dissertations

Weed control is a major problem that rice (Oryza sativa L.) producers face in the Mid-south every growing season. Restricted use of some rice herbicides has also highlighted the need for more research to be conducted on cultural weed control strategies and application technologies in rice. The aim of these studies was to evaluate rice weed control using various rice row widths, bed widths (irrigation-furrow spacing), rice cultivar selection, and nozzle type selection. Three field experiments were conducted in 2021 and 2022 across four sites at Lonoke, AR, Pine Tree, AR, Rohwer, AR, and Stoneville, MS. In experiment one, as …

Patterns In Winter Stonefly Distribution Along A River Continuum And Land-Use Gradient In Northwest Arkansas Streams, Zachary Tipton

Graduate Theses and Dissertations

Freshwater ecosystems are facing a crisis with extinction rates of aquatic species exceeding those of their terrestrial counterparts by up to fivefold. This decline is predominantly attributed to evolving land use patterns within watersheds, leading to chemical and physical transformations in freshwater habitats. Northwest Arkansas (NWA) represents one of the fastest-growing regions in the United States, undergoing substantial shifts in land use. Consequently, the status of aquatic life in this region remains uncertain. Addressing this concern, the latest Arkansas Wildlife Action Plan emphasizes the necessity of distribution and population data to guide conservation efforts for Species of Greatest Conservation Need …

A Framework Of Flow Models For The Development And Testing Of Cardiovascular Devices, Megan Laughlin

Graduate Theses and Dissertations

Cardiovascular disease (CVD) is the leading cause of death worldwide and its treatment continues to improve due to the advancement of innovative cardiovascular medical devices. In vitro and in silico models serve to replicate the in vivo environment for the development and testing of these devices prior to using animals for testing, and eventually humans in clinical trials. However, limitations related to these models exist in multiple areas including general, cardiac, and venous applications. Specifically, current gaps have been identified with customizable, tissue-mimicking phantoms (general), 2D Blood Speckle Imaging (BSI) (cardiac) and a bioprosthetic venous valve (venous). This dissertation focuses …

Characteristics Of Award-Winning Children’S Books About Agriculture: An Analysis Of Content, And The Perspectives Of Authors, Illustrators, And Publishers, Addison L. Beckham

Graduate Theses and Dissertations

The purpose of this two-article qualitative study was to characterize children's literature about agriculture and to describe the perceptions of authors and illustrators who are responsible for writing and designing these successful publications. This will result in the ability of organizations like Feeding Minds Press to provide writers, illustrators, and publishers with effective strategies and techniques to improve the accuracy and overall quality of children’s literature about agriculture. Few parameters exist for authors of children’s books about agriculture (Biser, 2007). These parameters are necessary to ensure the quality and accuracy of these educational efforts (Serafini, 2012). Though Feeding Minds Press …

Assessment Of Needs And Priorities For Precision Agriculture And Soil Testing In Arkansas, Solomon Frimpong

Graduate Theses and Dissertations

Optimum crop production is needed to meet the growing population's food, water, fiber, and energy needs. However, agricultural productivity is hampered by many destabilizing factors such as pest management challenges, declining water quantity, and climate change which threaten long-term food security. Maximizing productivity will require optimizing resources through Precision Agriculture (PA) by supplying inputs based on crop needs. Precision Agriculture offers tools that can be used to optimize crop management practices globally. However, stakeholders’ perceptions and the lack of data-driven site-specific recommendations limit stakeholders’ ability to fully harness the potential benefits of PA. Identification of stakeholder needs and approaches to …

Examining The Hormonal Underpinnings Of Affiliation Motivation In Oral Contraceptive Users, Hormonal Iud Users, And Naturally Cycling Women, Mikayla Tolliver

Graduate Theses and Dissertations

Affiliation is important for women during times of stress. Although, affiliation has been linked to women’s hormones across the menstrual cycle, many women use hormonal contraceptives which disrupt the links between hormones, psychology, and behavior. This study examined how hormonal contraceptives (hormonal IUD vs. the oral contraceptive pill) and menstrual cycle phase (follicular vs. luteal) are associated with affiliative motivations following the Trier Social Stress Test (TSST). Further, this study examined women’s cortisol reactivity and whether cortisol reactivity mediated affiliative motivation following the stressor. I found that oral contraceptive pill users projected more stress and rejection onto others’ faces and …

Geochemistry Of Metal-Rich Black Shales And Their Genetic Relationship To Mississippi Valley-Type Pb-Zn Ore Deposits, Midcontinent U.S., Noah S. Morris

Graduate Theses and Dissertations

The formation of Mississippi Valley-type (MVT) lead-zinc (Pb-Zn) ores is complex, involving an interaction of tectonic compression, hydrothermal fluid migration, and geochemical processes within the subsurface. Fundamental questions regarding this interaction remain unclear, including the source(s) of the Pb and Zn ions that make up the ores. A genetic link between black shales and MVT ores has been thought to exist because of (1) the presence of mature hydrocarbon and brine fluid inclusions within many MVT ores, (2) the association of the hydrothermal fluids that are linked to MVT ore formation with organic compounds and oil-like droplets, and (3) the …

Characterization Of Human Fibroblast Growth Factor 2 Variant To Determine Effects On Structure, Stability, And Cell Proliferation, Ryan Layes

Graduate Theses and Dissertations

Fibroblast growth factors (FGFs) are a family of cell signaling proteins conserved across multiple species. Each individual FGF elicits different cellular functions including, but not limited to, proliferation, migration, differentiation, angiogenesis, and wound healing. One of the most studied members, fibroblast growth factor 2 (FGF2), has demonstrated substantial wound healing capacity in a wide range of tissues including skeletal, muscular, neural, respiratory, epithelial, and cardiovascular. This ability makes FGF2 a potential therapeutic for a wide range of conditions and injuries. However, due to a short half-life at room temperature, therapeutic use of FGF2 is limited. It has been demonstrated that …

Evaluation Of Oxyfluorfen For Use In Roxy Rice, Casey Hunter Arnold

Graduate Theses and Dissertations

Mid-southern U.S. rice (Oryza sativa L.) producers are challenged every year to control problematic barnyardgrass [Echinochloa crus-galli (L.) Beauv.] and weedy rice (Oryza sativa L.) because they have evolved resistance to most labeled herbicides. Oxyfluorfen is a Herbicide Resistance Action Committee (HRAC)/Weed Science Society of America (WSSA) Group 14 herbicide that inhibits protoporphyrinogen oxidase within the plant. Currently, there are no HRAC/WSSA Group 14 herbicides labeled for barnyardgrass or weedy rice control in midsouthern rice production. Therefore, oxyfluorfen is being evaluated for barnyardgrass and weedy rice control. Plant breeders at the University of California – Davis were able to breed …

Demographics, Sexual Dimorphism, And Ecological Aspects Of Ambystoma Annulatum (Ringed Salamander) In Northwest Arkansas, Usa, Brian M. Becker

Graduate Theses and Dissertations

The need to study and monitor amphibian populations is increasing along with the threats to their population stability and persistence in nature. Northwest Arkansas is one of the fastest growing areas in the nation and with that growth comes rapid changes in land use, massive alterations to habitats, habitat loss, and the introduction of nonnative plants and animals. Ambystoma annulatum (Ringed Salamander) is an Ambystomatid endemic to the Ozark and Ouachita Mountains of southern Missouri, northern and western Arkansas, and eastern Oklahoma giving it a relatively small distribution compared to most Ambystoma. Therefore, Arkansas constitutes a significant portion of the …

Diminishing Creative Returns: Predicting Optimal Creative Performance Via Individual Differences In Executive Functioning, Kent F. Hubert

Graduate Theses and Dissertations

A robust finding in creativity research is that during idea generation, later (vs. earlier) responses on a divergent thinking task tend to be more creative, while the number of responses start off quickly, and then slow down over time, termed the serial order effect. Divergent thinking and time may follow a diminishing returns curve, where there is an optimal amount of time that should be spent while generating ideas. Executive functions (inhibition, updating, and shifting) have been associated with divergent thinking, specifically with the idea generation phase, but the effect of individual differences on the temporal dynamics of divergent thinking …

Amyloid Fibrils Of Human Fgf-1 Induced By Different Detergents, Zeina Ismael Ibrahem Alraawi

Graduate Theses and Dissertations

Nature achieves molecular self-assembly through the ordered growth of nanoscale building blocks with high efficiency to fabricate macromolecular architectures. One example of self- assembly is peptides folding onto protein is one of the most astounding biological self-assembly processes. When proteins aggregate to form amyloid fibers, the secondary structure of the protein converts from its native state to a cross-beta-sheet. Fibroblast growth factors (FGFs) possess an essential role in neuronal survival during development. In addition, they are involved in neural stem cell (NSC) proliferation. Fibroblast growth factors (FGFs) are well known to be synthesized in the central nervous system (CNS) and …

The Spatial And Temporal Distribution Of Large Rock Blocks And Control On Landscape Evolution In The Ozarks, Chelsea Moran

Graduate Theses and Dissertations

Geologists often use landscape form to infer landscape processes through time. While climate and tectonics shape geomorphic form, the potential range of spatial or temporal scales that can shape any specific landscape can render landscape process-form based hypotheses too general for consideration. Contributions by mathematical modeling have helped bridge the gap between inferring processes from form, notably in how sediment transport dynamics shape hillslopes. However, few models encapsulate the movement of large rock blocks ( >2 meters across) and their potential impact as hillslope sediment transport disruptors. The Upper Buffalo River watershed (BRW) in the Ozarks of northern Arkansas has …

A Generic Qualitative Inquiry Of The Challenges For Black African American Men Who Have Experienced Trauma, Randall Lee Maurice Shakir

Graduate Theses and Dissertations

Black African American Men (BAAM) suffer disproportionately from trauma related challenges and have a higher risk of encountering trauma across the lifespan. The negative impact of trauma is a major public health concern in the United States, evidence suggests trauma negatively impacts the physical well-being, mental health, and mortality rate. BAAM have increased rates of trauma exposure and their traumatic experience is historically complex involving a variety of contemporary issues (i.e. Adverse Childhood Experiences (ACEs), depression, victimization and desensitization, stress). This research aims to explore the complex nature of their trauma-related challenges among a purposeful sample of BAAM participants in …

In Vitro And In Vivo Evaluation Of Antimicrobial Activity, Oocyst Sporulation Rate, Oocyst Integrity And Oocyst Infectivity In Eimeria Maxima M6 Oocyst Suspensions Treated With Chlorhexidine Salts In Comparison With Potassium Dichromate, Lauren Laverty

Graduate Theses and Dissertations

Eimeria species oocysts cause coccidiosis, one of the costliest diseases in the poultry industry. Coccidiosis infections cause significant economic losses due to reduced performance in chickens and turkeys. The objective of this thesis was to determine fecal bacterial recovery from chlorhexidine (CHX) salt suspensions containing Eimeria maxima M6 oocysts and evaluate the effect of CHX salts on oocysts during long-term cold storage. For bacterial recovery experiments, CHX digluconate and CHX gluconate were tested at different concentrations on poultry fecal slurries during oocyst shedding. Eimeria maxima M6 oocysts were evaluated in CHX salt suspensions to test the effect on oocyst degradation …

Geospatial Analysis Of Weathering And Recession On Architectural Limestone At The 1891 Powerhouse/Icehouse In Eureka Springs, Arkansas, Stuart Bruce Wright

Graduate Theses and Dissertations

The objective of this study of the architectural limestone of the 1891 Powerhouse/Icehouse in Eureka Springs, Arkansas was to analyze the environmental factors that influenced the deterioration and surface recession of this historical stone structure. The focus of this research was to examine and establish a baseline study that may assist in future monitoring and documentation of this historic limestone structure on stone weathering. A replicable methodology was specifically created toward this baseline research in hopes that it represented a solid baseline study. Observations and data analyses revealed the relationship between the architectural limestone building structure and the dominating recession …