Digital Commons Network^™

Leptin Decreases Gluconeogenesis And Gluconeogenic Substrate Availability In Patients With Lipodystrophy, Emmanuel Quaye, Shaji Chacko, Megan Startzell, Rebecca J Brown

Children’s Nutrition Research Center Staff Publications

Context: The effects of leptin, an adipocyte-derived hormone that signals overall energy sufficiency, can only be studied in leptin-deficient conditions. In patients with lipodystrophy, a rare disease and unique model of leptin deficiency, treatment with recombinant leptin (metreleptin) improves glycemia and decreases energy expenditure. We hypothesized that these improvements might be mediated by reduced gluconeogenesis (GNG), an energy-requiring process.

Objective: To determine the effects of metreleptin on GNG and GNG substrates.

Methods: This was a single-arm prospective study of metreleptin administration in 15 patients with lipodystrophy, 9 of whom had data on GNG (NIH, 2013-2018). We analyzed total GNG, insulin-mediated …

Ints6 And The Integrator Phosphatase Module Tune The Efficiency Of Select Premature Transcription Termination Events, Rina Fujiwara, Si-Nan Zhai, Dongming Liang, Aayushi P Shah, Matthew Tracey, Xu-Kai Ma, Christopher J Fields, María Saraí Mendoza-Figueroa, Michele C Meline, Deirdre C Tatomer, Li Yang, Jeremy E Wilusz

Children’s Nutrition Research Center Staff Publications

The metazoan-specific Integrator complex catalyzes 3' end processing of small nuclear RNAs (snRNAs) and premature termination that attenuates the transcription of many protein-coding genes. Integrator has RNA endonuclease and protein phosphatase activities, but it remains unclear if both are required for complex function. Here, we show IntS6 (Integrator subunit 6) over-expression blocks Integrator function at a subset of Drosophila protein-coding genes, although having no effect on snRNAs or attenuation of other loci. Over-expressed IntS6 titrates protein phosphatase 2A (PP2A) subunits, thereby only affecting gene loci where phosphatase activity is necessary for Integrator function. IntS6 functions analogous to a PP2A regulatory …

Towards Ethical Artificial Intelligence In Universities: Chatgpt, Culture, And Mental Health Stigmas In Asian Higher Education Post Covid-19, Michael James Day

Journal of Technology in Counselor Education and Supervision

Mental health can be interpreted as a social taboo in Asia, ensuring that students with mental health stigmas (SWMHS) face complex educational journeys that impact their wellbeing. This article provides a conceptual interdisciplinary commentary that illustrates how in Asian higher education (HE) settings, the psychosocial phenomena of face culture, a sociolinguistic blend of high-context power relations, sense-making, and cultural capital, defines human-to-human (HTH) dialogue. It suggests that human-to-computer-interaction (HCI) through artificial intelligence (AI) technologies, such as ChatGPT, could improve university wellbeing strategies in Asia. The article situates AI discussion into the sociolinguistic features of face culture in Thailand and China …

Immunosequencing Applications In Cutaneous T-Cell Lymphoma, Jenna Mandel, Laura Gleason, Daniel Joffe, Safiyyah Bhatti, Neda Nikbakht

Department of Dermatology and Cutaneous Biology Faculty Papers

Immunosequencing has emerged as a newer clinical test for assessment of T-cell clonality in the blood and skin of cutaneous T-cell lymphoma (CTCL) patients. Utilization of immunosequencing, also known as high-throughput sequencing of the T-cell receptor (HTS-TCR), enables identification and quantification of the precise genetic signature of dominant T-cell clones. Although immunosequencing is more sensitive than commonly used methods such as polymerase chain reaction (PCR) paired with capillary electrophoresis or flow cytometry, it remains underutilized for CTCL management. Nonetheless, incorporation of HTS-TCR in clinical practice offers distinct advantages compared to other molecular analyses that may improve diagnostic evaluation, prognostication, and …

Parallel Neural Pathways Control Sodium Consumption And Taste Valence, Yameng Zhang, Allan-Hermann Pool, Tongtong Wang, Lu Liu, Elin Kang, Bei Zhang, Liang Ding, Kirsten Frieda, Richard Palmiter, Yuki Oka

Faculty, Staff and Student Publications

The hedonic value of salt fundamentally changes depending on the internal state. High concentrations of salt induce innate aversion under sated states, whereas such aversive stimuli transform into appetitive ones under sodium depletion. Neural mechanisms underlying this state-dependent salt valence switch are poorly understood. Using transcriptomics state-to-cell-type mapping and neural manipulations, we show that positive and negative valences of salt are controlled by anatomically distinct neural circuits in the mammalian brain. The hindbrain interoceptive circuit regulates sodium-specific appetitive drive , whereas behavioral tolerance of aversive salts is encoded by a dedicated class of neurons in the forebrain lamina terminalis (LT) …

Metalloproteomic Investigation Of Hg-Binding Proteins In Renal Tissue Of Rats Exposed To Mercury Chloride, Emerson Carlos De Almeida, Victor Diego Faria, Felipe Dalmazzo Cirinêu, Maria G.A. Santiago, Beatriz Miotto, José C.S. Vieira, Camila Pereira Braga, Jiri Adamec, Ana A.H. Fernandes, Marília A.R. Buzalaf, Pedro De Magalhães Padilha

School of Medicine Faculty Publications

Results obtained from rat studies indicate that, even at low concentrations, mercurial species cause harmful effects on the kidneys, by inducing the nephrotic oxidative stress response. In the present work, Hg-associated proteins were identified as possible mercury-exposure biomarkers in rat kidneys exposed to low mercury chloride concentrations for 30 days (Hg-30) and 60 days (Hg-60), using metalloproteomic strategies. The renal proteomic profile was fractioned by two-dimensional electrophoresis and the mercury determinations in kidney samples, protein pellets and protein spots were performed using graphite furnace atomic absorption spectrometry. The characterization of Hg-associated protein spots and the analysis of differentially expressed proteins …

Artificial Intelligence For Mental Health Support During Covid-19: Experiences Of Graduate Counseling Students, Russell Fulmer, Eric T. Beeson, Carl Sheperis, Daniel Rosello, Rebecca Edelman

Journal of Technology in Counselor Education and Supervision

The purpose of this study was to examine how an AI chatbot could provide mental health support to counselors-in-training during the COVID-19 pandemic. The chatbot “Tess” was available to participants for two weeks. Participants responded to questions about their experience and the content of this qualitative data was analyzed. Themes emerged that focused on mental health during the pandemic, utility of the AI chatbot during the pandemic, and potential therapeutic use in general. Findings were mixed and suggest some skepticism among counseling students towards the use of an AI chatbot.

Technology Justice: Taxation Of Our Collective And Cumulative Cognitive Inheritance, Hilary G. Escajeda

Loyola of Los Angeles Law Review

As artificial intelligence and robotic technologies accelerate economic transformation, outdated property and tax laws will increasingly fail American workers with ordinary skills that perform routine job functions. Because technology may render millions of workers redundant, U.S. policymakers must make significant social, economic, and legal structural changes to (1) improve the lives of average workers, (2) support the economy, and (3) maintain political stability.

Inspired by Thomas Paine’s Agrarian Justice, this twenty-first century Article argues that “Technology Justice” requires that humans benefit from the cognitive endowment created by our ancestors’ minds. Specifically, it asserts that our collective and cumulative cognitive …

Cárcel De Amor / The Prison Of Love, Laura Francis, Álvaro Garrote Pascual

Medieval Feminist Forum: A Journal of Gender and Sexuality

No abstract provided.

Comparative Analysis Of Rectum Delineation: Inter-Observer Agreement Among Treatment Planners Versus Auto Segmentation In A Single Institution, Haley Douglas

Masters Projects

Abstract

Introduction

The past 20 years have seen a continuous rise in the cancer patient population, resulting in staffing shortages, longer wait times for patients, and potentially lower-quality care. Artificial intelligence has been incorporated into the healthcare system and has provided ease with understaffing. Using AI technology to delineate structures can reduce planning time and variation between planners. The objective of this research is to assess the level of consistency in the rectum boundary delineation performed by different dosimetrists working at a single institution, as compared to the performance of the AutoContour software. This analysis will help determine the efficacy …

The Involvement Of Hepatic Cytochrome P450s In The Cytotoxicity Of Lapatinib, Si Chen, Xilin Li, Yuxi Li, Xiaobo He, Matthew Bryant, Xuan Qin, Feng Li, Ji-Eun Seo, Xiaoqing Guo, Nan Mei, Lei Guo

Faculty, Staff and Students Publications

Lapatinib, an oral tyrosine kinase inhibitor used as a first-line treatment for HER2-positive breast cancer, has been reported to be associated with hepatotoxicity; however, the underlying mechanisms remain unclear. In this study, we report that lapatinib causes cytotoxicity in multiple types of hepatic cells, including primary human hepatocytes, HepaRG cells, and HepG2 cells. A 24-h treatment with lapatinib induced cell cycle disturbances, apoptosis, and DNA damage, and decreased the protein levels of topoisomerase in HepG2 cells. We investigated the role of cytochrome P450 (CYP)-mediated metabolism in lapatinib-induced cytotoxicity using our previously established HepG2 cell lines, which express each of 14 …

Current Biomedical Applications Of 3d-Printed Hydrogels, Allan John R Barcena, Kashish Dhal, Parimal Patel, Prashanth Ravi, Suprateek Kundu, Karthik Tappa

Faculty, Staff and Student Publications

Three-dimensional (3D) printing, also known as additive manufacturing, has revolutionized the production of physical 3D objects by transforming computer-aided design models into layered structures, eliminating the need for traditional molding or machining techniques. In recent years, hydrogels have emerged as an ideal 3D printing feedstock material for the fabrication of hydrated constructs that replicate the extracellular matrix found in endogenous tissues. Hydrogels have seen significant advancements since their first use as contact lenses in the biomedical field. These advancements have led to the development of complex 3D-printed structures that include a wide variety of organic and inorganic materials, cells, and …

Mir-410 Is A Key Regulator Of Epithelial-To-Mesenchymal Transition With Biphasic Role In Prostate Cancer, Diana M Asante, Amritha Sreekumar, Sandip Nathani, Tae Jin Lee, Ashok Sharma, Nikhil Patel, Matthew N Simmons, Sharanjot Saini

Faculty, Staff and Student Publications

The molecular basis of prostate cancer (PCa) progression from the primary disease to metastatic castration-resistant prostate cancer (CRPC) followed by therapy-induced neuroendocrine prostate cancer is not fully understood. In this study, we elucidate the role of miR-410, a little-studied microRNA located on chromosome 14q32.31 within the DLK1-DIO3 cluster, in PCa. miR-410 expression analyses in primary and metastatic PCa tissues and cell lines show that its levels are decreased in initial stages and increased in advanced PCa. Functional studies were performed in a series of PCa cell lines. In LNCaP cells, miR-410 overexpression led to decreases in cellular viability, proliferation, invasiveness, …

Olanzapine Pharmacokinetics: A Clinical Review Of Current Insights And Remaining Questions, Priyanka Kolli, Grace Kelley, Marianela Rosales, Justin Faden, Ryan Serdenes

Department of Psychiatry and Human Behavior Faculty Papers

Olanzapine is one of the most widely used antipsychotics since its initial approval by the US Food and Drug Administration in 1996 and has undergone extensive pharmacokinetic study. Despite being utilized in clinical psychiatry for decades, there remain questions regarding the variety of available formulations, the utility of therapeutic drug monitoring, altered kinetic properties in special populations/medical illnesses, the use of high-dose olanzapine, and drug interactions, among many others. We performed a narrative literature review of olanzapine pharmacokinetics in June 2023 using the US National Library of Medicine's PubMed.gov resource (https://www.ncbi.nlm.nih.gov/pubmed) and Google Scholar. Herein, we review clinically relevant aspects …

Distinct Gene Expression Signatures Comparing Latent Tuberculosis Infection With Different Routes Of Bacillus Calmette-Guérin Vaccination, Richard F Silver, Mei Xia, Chad E Storer, Jessica R Jarvela, Michelle C Moyer, Azra Blazevic, David A Stoeckel, Erin K Rakey, Jan M Tennant, Johannes B Goll, Richard D Head, Daniel F Hoft

2020-Current year OA Pubs

Tuberculosis remains an international health threat partly because of limited protection from pulmonary tuberculosis provided by standard intradermal vaccination with Bacillus of Calmette and Guérin (BCG); this may reflect the inability of intradermal vaccination to optimally induce pulmonary immunity. In contrast, respiratory Mycobacterium tuberculosis infection usually results in the immune-mediated bacillary containment of latent tuberculosis infection (LTBI). Here we present RNA-Seq-based assessments of systemic and pulmonary immune cells from LTBI participants and recipients of intradermal and oral BCG. LTBI individuals uniquely display ongoing immune activation and robust CD4 T cell recall responses in blood and lung. Intradermal BCG is associated …

The Trader Joe’S Way (To Success), Ritch Eich, Joan Taylor Cummings Eich

The Journal of Values-Based Leadership

No abstract provided.

Top Directed, Bottom Driven: Subaru Tackles Waste (Interview With Denise Coogan, Subaru), Elizabeth Gingerich

The Journal of Values-Based Leadership

No abstract provided.

Industrial Education 4.0: The Role Of Human, Technology, And Data Literacy, Suparno Suparno, Dedi Purwana, Agus Wibowo, Bagus Shandy Narmaditya

Journal of Media Literacy Education

This study aims to confirm the effect of industrial education 4.0 on data, technology, and human literacy among postgraduate students in Indonesia. The research adopted covariance-based structural equation modeling by employing AMOS 25 to examine the relationship between variables. The model estimation was performed using confirmatory factor analysis as a standard model for measuring industrial education 4.0 and involved structural model analysis to confirm the hypotheses. The population in this research paper was 2.958 postgraduate students in 32 study programs, while the sample was 312 respondents, which was determined using the proportional random sampling technique. This study tested the three …

Library Steam Kits: Developing Circulatable Curriculum For Community Steam Learning, Daphne Fauber, Ashley Fletcher

Purdue Journal of Service-Learning and International Engagement

Public libraries serve as repositories for a movement described as cultivation of the Library of Things. In the wake of COVID-19, the West Lafayette Public Library enhanced its existing Library of Things collection through the creation of science, technology, engineering, art, and mathematics (STEAM) kits. Since 2017, the West Lafayette Public Library has held regular free STEAM programs for the community; those programs were put on hold during the height of the COVID-19 pandemic, which concurred with a library renovation. These kits provide the community with the ability to learn STEAM concepts at home engaging, hands-on activities that may otherwise …

Warming Up Cold Cases With Dr. Krystal Hans, Vanessa Cooper

Purdue Journal of Service-Learning and International Engagement

Dr. Krystal Hans is an assistant professor of forensic entomology in the Department of Entomology. Dr. Hans teaches courses on forensic science and forensic entomology, including the “Forensic Investigation” (ENTM 22810) course, where a major component of the course is a cold case project. The cold case project focuses on accessing information about an unresolved case and trains students to engage with empathy as advocates for victims while developing skills in research, digital and information literacy, submission of a Freedom of Information Act (FOIA) request, designing infographics, and public speaking. Students produce digital case files and infographics to display the …

The Complexities Of Green Infrastructure, Troy Weber

Purdue Journal of Service-Learning and International Engagement

The Wabash River is the most extensive river system in Indiana. The river drains two-thirds of Indiana’s 92 counties, which can have issues associated with stormwater runoff (Weist, 1981). As precipitation runs off impervious surfaces, it can carry high concentrations of pollutants, such as excess nitrogen and phosphorus, pet waste, and automobile fuel. Prior to impervious runoff entering the river, preexisting contaminants include industrial waste (acid mine drainage and oil field brines), municipal waste, and sediment (Weist, 1981). Thus, stormwater runoff can negatively impact the Wabash River watershed. On top of this pollution, Lafayette and West Lafayette have combined sewer …

Stimulating The Interest Of Young Learners In Physics Through Storytelling And Stem Activities, Emmanuel Babalola, Wonjin Yu, Hillary Ongoyo Omoze, Zainab Saka, Favour Ojike, Iyanu Adedeji, Zhu Yue

Purdue Journal of Service-Learning and International Engagement

The Physics is Fun Project is an initiative funded by Purdue’s Office of Engagement and aims to promote an interest in physics among young learners aged 5 to 12. The project’s unique approach blends instructional design and motivational design to create an engaging and relevant learning experience. Development of the project was guided by the analyze, design, develop, implement, and evaluate (ADDIE) framework and Keller’s attention, relevance, confidence, and satisfaction (ARCS) theory and utilized storytelling and hands-on learning strategies to bring physics to life through the story of a young physics enthusiast who is passionate about building a dream car. …

Service-Learning In Action: Lafayette, Indiana, Rain Garden Installation, Elizabeth Mccleery

Purdue Journal of Service-Learning and International Engagement

Indiana’s Wabash River is being polluted with contaminated water runoff from precipitation events. A lack of pervious land cover has led to an accumulation of fertilizer, sediment, and waste in the river. Green infrastructure, a stormwater management practice that mimics the natural ecosystem, is one of the most effective ways to prevent pollution from stormwater runoff and benefit the community. This project consisted of a rain garden installation at the Lafayette Fueling Station, a site where frequent water drainage and water runoff into the Wabash occurs. The rain garden will allow on-site water infiltration during rain events and promote natural …

Reverse Pseudo-Gours: A New Sub-Type Of Folia Observed In The Nerja Cave (Se Spain), Cristina Liñán Baena, Concepción Jiménez De Cisneros, Yolanda Del Rosal, Paolo Forti

International Journal of Speleology

A new sub-type of folia named “reverse pseudo-gour” has been observed and described in the Nerja Cave, southern Spain. It consists of fairly vertical, thin barriers (about 5 mm high and 2 mm thick) that develop on the underside of a sub-horizontal surface (shelfstone) and grow in the opposite direction to normal gours (rimstone dams), generating sinuous shapes. Their mineral composition is essentially calcium carbonate, although globular aggregates composed of clay and phosphate minerals have also been identified. The genesis and evolution of these reverse pseudo-gours occur just at the air-water interface and are controlled by (1) the sub-horizontality of …

دور المنصات التعليمية اإللكترونية في تعزيز ثقافة التعليم األخضر ومعوقات نشرها من وجهة نظر المعلمين في األردن, Haitham Abd-Alkareem Al Horany, Fatima Wahba Dr

Journal of the Association of Arab Universities for Research in Higher Education مجلة اتحاد الجامعات العربية للبحوث في التعليم العالي

هدف المقال التعرف إلى دور المنصات التعليمية اإللكترونية في تعزيز ثقافة التعليم األخضر ومعوقات نشرها من وجهة نظر المعلمين في األردن، وقد تكونت عينة الدراسة من )292( معلماً ومعلمة ممن يعملون في المدارس الحكومية األردنية، وقد تم اختيارهم ، عشوائيا ولتحقيق أهداف الدراسة استخدم المنهج ً )الوصفي التحليلي(، واستخدمت االستبانة كأداة لجمع بيانات هذه الدراسة. وقد أظهرت نتائج الدراسة أن دور المنصات التعليمية اإللكترونية في تعزيز ثقافة التعليم األخضر من وجهة نظر المعلمين كانت بدرجة متوسطة على األداة ككل، وعلى كل مجال من مجاالت األداة، كما تبين عدم وجود فروق ذات داللة إحصائية عند مستوى الداللة )0.05=α )تعزى ألثر الجنس، …

أثر استراتيجية مقترحة تدمج ما بين استراتيجيتي التعليم المدمج والتعليم المعكوس في معالجة ضعف القراءة لدى طلبة الصف الثالث األساسي وفي تنمية تفكيرهم الناقد, Ola Habes Alreqeb

Journal of the Association of Arab Universities for Research in Higher Education مجلة اتحاد الجامعات العربية للبحوث في التعليم العالي

هدف المقال الكشف عن أثر استراتيجية مقترحة تدمج ما بين استراتيجيتي التعليم المدمج والتعليم المعكوس في معالجة ضعف القراءة لدى طلبة الصف الثالث األساسي وفي تنمية تفكيرهم الناقد، واستخدمت الباحثة المنهج شبه التجريبي، ولتحقيق أهداف الدراسة تم تطوير اختبار شفوي ومقياس تفكير ناقد، وذلك بعد التأكد من صدقهما وثباتهما، وتكونت الدراسة من مجموعتين ضابطة وتجريبية بحيث تم توزيع مجموعتي الدراسة عشوائًيا، بمعدل )25( طالب وطالبة من الصف الثالث األساسي في كل شعبة، وأشارت نتائج التحليل اإلحصائي إلى وجود فروق ذات داللة إحصائية بين متوسطات أداء مجموعتي الدراسة باختالف طريقة التدريس )استراتيجيتي التعليم المدمج والتعليم المعكوس، والطريقة االعتيادية(، في مبحث اللغة …

أثر استخدام المحاكاة في تدريس العلوم للمرحلة الأساسيّة العليا على الدافعيّة نحو التّعلّم ومهارات التّفكير العلميّ, Alaa Adnan Smiek, Mohammad Habib Samkari

Journal of the Association of Arab Universities for Research in Higher Education مجلة اتحاد الجامعات العربية للبحوث في التعليم العالي

هدفت المقال التعرفِ على أثَر استخْدامِ المحاكاةِ في تَدريسِ العُلوم للمرحلةِ الأساسيَّةِ العُلْيا على الدّافعيّةِ نحوَ التَّعلُّمِ ومهارات التَّفكير العِلمِيَّ، وأُجريتْ هذه الدراسة في مجموعةِ مدارس الجامعة (الأولى، والثانية) في محافظة العاصمة عمان في الأردن، بالفصلِ الدراسيّ الثاني من العامِ الدراسيّ 2021-2022. ولتحقيقِ أهداف الدراسة استُخدِم المنهج شبه التجريبي، حيث تمَّ اختيار أفراد الدراسة بالطريقة القصديّة، والّتي تكونتْ من (60) طالبة من طلبة الصف العاشر الأساسي، حيثُ تكونتِ العينةُ من ثلاثِ مجموعات تمَّ توزيعها عشوائيًا: التجريبية الأولى دُرّست باستخدام تطبيق (PhET)، والمجموعة التجريبية الثانية دُرستْ باستخدام تطبيق (Crocodile Physics)، والأخيرة مجموعة ضابطة دُرِّسَت بالطريقةِ الاعتياديةِ، وتكونت كل شعبة من (20) …

Importance Of Employee Motivation Across Different Organizational Landscapes, Lorena Monserrat Tenorio

CISLA Senior Integrative Projects

No abstract provided.

Environmental Footmark And Its Effects On The Sustainability Of Towns, Ali Abdul Samea Hameed

Al-Esraa University College Journal for Engineering Sciences

The Ecological Footmark (EF) is one of the utmost recent and important criteria for assessing the capacity of the population to uphold a life sustainable way. It tests the extent which the indicators have an influence on the natural resources (NR) of the ecosystem such as forestry, water bodies, agricultural lands, pastures, CO2, and properties for construction purposes. This footmark was utilized to evaluate the use of NR to the capacity of the land for regeneration. This research paper discusses the theoretical conceptual component of the EF framework and plans regarding issues related to the environment. The environmental image (EI) …

Some Biological Markers In Diabetic Patients, Ahmed Malk Hassan, Hasan Ali Hussein, Radhwan Mohmmed Hussein, Maryam Hiyder Kadhum

Al-Esraa University College Journal for Medical Sciences

Endstage renal disease (ESRD) usually comes on by diabetic nephropathy (DN) and is one of the most dreaded chronic microvascular complications of diabetes. The objective of this study is to provide an overview of the DN-biomarker landscape with a particular emphasis on new approaches that improve the sensitivity of biomarkers for identifying patients who will develop DN or who are at risk of developing ESRD. This study was designed as a case-control study. 120 individuals were involved, divided into three groups: a control group diabetic without nephropathy, Early stage and end stage of nephropathy, 40 patients were taken for each …