Digital Commons Network^™

Effects Of Short-Term Pesticides Exposure On Swimming Behavior, Tissue Morphology, Oxidative-Nitrative Stress, Antioxidant Expression, And Cellular Apoptosis In Kidneys Of Goldfish, Esmirna Cantú

Theses and Dissertations

The prevalence of pesticides in the aquatic environment continues to increase due to anthropogenic activities and poses a threat to aquatic organisms. The aim of this study was to determine the short-term exposure (one-week) of pesticide mixtures (metolachlor, linuron, isoproturon, tebucanazole, alconifen, atrazine, pendimethalin; azinphos-methyl) on swimming behaviors, kidney morphology, and expression of nitrotyrosine protein (NTP), dinitrophenyl protein (DNP), superoxide dismutase (SOD), catalase (CAT), renin, and cellular apoptosis in goldfish (Carassius auratus). Results indicated that both low- and high-dose pesticide mixtures decreased the distance swimming behavior. Histological analysis showed pesticide exposure alters kidney morphology. DNP, NTP, and renin expressions, and …

The Impacts Of Caregiving And Social Support On Stress For Multiple Sclerosis: A Study On Informal Caregivers, Jacqueline Mercado

Theses and Dissertations

The purpose of the present quantitative study was to gain an increased understanding of the impacts of caregiving and social support on stress for unpaid informal caregivers of persons with Multiple Sclerosis. Caregiving for MS can be rewarding yet stressful. Informal caregivers are the primary source of ongoing home care for family members or friends with MS. Due to the complexity of care, caregivers are especially vulnerable to higher strain as they often feel unprepared for this role. This study examined perceived social support and demographic variables on caregivers' stress to gain insight into mediating caregiver psychosocial challenges. …

Publication Of Undergraduate Experiments, Development Of Additional X-Ray Crystallography Experiments, And Proof-Of-Concept Cyclopropanation Reactions, Alain Beauparlant

Electronic Theses and Dissertations

Single crystal X-ray crystallography instructional materials were developed using a variety of compounds including sugar, Epsom salt, pyrite, copper(II) acetate, and rhodium(II) acetate. Rhodium(II) acetate was reacted with a variety of functionalized benzonitriles, and when crystals were obtained, characterized using single crystal X-ray crystallography. Rhodium(II) acetate with adducts of 4-nitrobenzonitrile and 4-(dimethylamino)benzonitrile were also characterized using proton NMR spectroscopy and Fourier Transform Infrared Spectroscopy. Rhodium(II) acetate with adducts of 4-(dimethylamino)benzonitrile was used as a catalyst in a proof-ofconcept reaction to determine if it acts as a catalyst in cyclopropanation reactions. Further, the new rhodium(II) acetate dinitrile compounds were investigated to …

Synthesis Of Bacterial Glycerophospholipids For Biomembrane Model Studies: A Means To Advanced Biofuels, Felix Adulley

Electronic Theses and Dissertations

To reduce reliance on fossil fuels, sustainable biofuels are being pursued, especially advanced biofuels like 1-butanol that have higher energy content and greater compatibility with existing infrastructure than ethanol. A persistent challenge is the yield-limiting toxicity of biofuels and process solvents, such as tetrahydrofuran, to the microbes that ferment biomass into biofuel. The cell membrane is a focal point of toxicity, and understanding how it interacts with fuels and solvents is key to improving yield. Phospholipid bilayers are the core of biomembranes, and model biomembranes of defined composition provide the ideal platform for biophysical studies. To this end, glycerophospholipids characteristic …

Intestinal Microbiome, Fecal Fermentation Profile, And Health Indices In Hiv Infected Men Versus Non-Infected Controls, Mary Andreae, Mary C. Andreae Mrs

Electronic Theses and Dissertations

Many HIV-positive (HIV+) males on Highly Active Anti-Retroviral Therapy (HAART) experience metabolic abnormalities, including Non-Alcoholic Fatty Liver Disease (NAFLD) and lipodystrophy. The intestinal microbiota and short chain fatty acids (SCFA), participate in bidirectional communication with their host. Dysbiosis in HIV+ males on HAART demonstrate a Prevotella-rich enterotype shaped by multiple factors including, medications, adiposity, diet, intestinal permeability, and lifestyle; our objective was to investigate these factors. 19 HIV+ and 21 HIV- males were enrolled. BMI and hip-to-waist ratio (H:W) were obtained, and FibroScan for liver health. Intestinal permeability markers Claudin-21, flagellin, and intestinal fatty acid binding protein (IFABP) in …

Comparative Analysis Of Teacher Effects Parameters In Models Used For Assessing School Effectiveness: Value-Added Models & Persistence, Merlin J. Kamgue

Graduate Theses and Dissertations

Longitudinal measures for students have become increasingly popular to estimate the effects of individual teachers and schools. Value-added models are one of the approaches using longitudinal data to evaluate teachers and schools. In the value-added model (VAM) literature, many statistical approaches have been developed and used to estimate teacher or school effects on student learning. This study opted to use a Bayesian multivariate model for evaluating teacher effects. The generalized persistence models can handle longitudinal data, not vertically scaled, allowing for a below-par teacher’s effects correlation across test administrations. This study first generated longitudinal students’ test score data and used …

Effect Of Celery (Apium Graveolens L.) Microgreens On Drosophila Melanogaster, Mohamad Agus Salim, Muhammad Subandi, Yeni Yuniarti

Baghdad Science Journal

Celery (Apium graveolens L. ; family : Apiaceae) was often used as a spice in daily food. However, this plant contains many antioxidant compounds useful for attenuating neurodegenerative disorders including Parkinson's disease. Planting celery in the form of microgreens harvested 15 days was expected to increase the content of bioactive compounds. In the current study, we intended to evaluate the neuromodulatory potential of methanol extract of celery microgreens on fruit flies (Drosophila melanogaster Meigen : family Drosophilidae ; ordo : Diptera) which were exposed to paraquat. Neuroprotective capacity was assessed by survival rate, locomotor performance, lipid peroxidation and dopamine content …

Microbial Quality Of Paddy Fields At Alfurat Alawsat Area, Iraq And The Effect Of Milling Process On The Rice Contamination Level, Abeer S. Alhendi, Aqeel M. Ali, Abeer K. Mohammed

Baghdad Science Journal

Fifty-Four paddy samples, 45 soil samples, and nine irrigation water samples of some paddy fields at Alfurat Alawsat Area, Iraq were collected to investigate the microbial quality including total count bacteria (TCB), molds and yeast, total coliform (TC), and E. coli bacteria. Paddy samples were processed at the laboratory level to produce rice at 32 and 36 whiteness, and the microbial quality of the produced rice was conducted too. The results showed that all irrigation water samples were overpassed the FDA limit for E. coli. All paddy samples were exceeded the good limit of TCB, but no sample exceeded the …

Effect Of Chromium (Vi) On The Oxidation Of Methylene Blue Dye By Fe3o4/ Chitosan Composite, Yaman K. Sadiq, Khulood A. Saleh

Baghdad Science Journal

Heavy metal ion removal from industrial wastewater treatment systems is still difficult because it contains organic contaminants. In this study, functional composite hydrogels with photo Fenton reaction activity were used to decompose organic contaminants. Fe3O4 Nanoparticle, chitosan (CS), and other materials make up the hydrogel. There are different factors that affected Photo-Fenton activity including (pH, H2O2 conc., temp., and exposure period). Atomic force microscopy was used to examine the morphology of the composite and its average diameter (AFM). After 60 minutes of exposure to UV radiation, CS/ Fe3O4 hydrogel composite had degraded methylene blue (M.B.) dye by 92 percent. In …

Gene Expression And Methylation Levels Of Pcsk9 Gene In Iraqi Patients With Coronary Artery Disease, Marwa M. Al-Attar, Salwa J. Al-Awadi Al-Awadi, Shaimaa Y. Abdulfattah

Baghdad Science Journal

The expression of the Proprotein Convertase Subtilisin/Kexin Type 9 gene (PCSK9) is inextricably related to lipid levels and a risk of atherosclerotic coronary artery disease (ASCAD). The present study aims to measure the quantity of PCSK9 gene expression and the effect of methylation on its expression level taking part in the pathogenesis of acute coronary artery disorder. A current study included 150 subjects from the Iraqi population, 100 ASCAD patients and 50 healthy controls. The concentration of PCSK9 in each serum sample was determined by the ELISA technique, the expression levels of the PCSK9 gene in whole blood were estimated …

Antioxidant Activity Of Endophytic Bacteria Isolated From (Pyrrosia Piloselloides) (L) M.G. Price, Achmad Arifiyanto, Salman Farisi

Baghdad Science Journal

Endophytic bacteria produced analogous secondary metabolites of their hosts. Similarly, the ability to generate antioxidants is not an exception. Dragon scales (Pyrrosia piloselloides), an epiphytic plant of the Polypodiaceae family, are frequently overlooked. This research aims to isolate antioxidant-producing bacteria from dragon-scale fern leaves. The antioxidant activities were tested after the extraction procedure using ethanolic extract. Bacteria were characterized and selected as candidates for antioxidant production by screening for the production of total phenolic compounds. Antioxidant levels were determined utilizing the ABTS, FRAP, and DPPH techniques. The preliminary findings of the entire phenolic compound test revealed that isolates B2, B3, …

Simultaneous Determination Of Binary Mixture Of Estradiol And Progesterone Using Different Spectrophotometric Methods, Dashty K. Ali, Nabil A. Fakhre, Chnar M. Rasheed

Baghdad Science Journal

Four rapid, accurate and very simple derivative spectrophotometric techniques were developed for the quantitative determination of binary mixtures of estradiol (E2) and progesterone (PRG) formulated as a capsule. Method I is the first derivative zero-crossing technique, derivative amplitudes were detected at the zero-crossing wavelength of 239.27 and 292.51 nm for the quantification of estradiol and 249.19 nm for Progesterone. Method II is ratio subtraction, progesterone was determined at λmax 240 nm after subtraction of interference exerted by estradiol. Method III is modified amplitude subtraction, which was established using derivative spectroscopy and mathematical manipulations. Method IIII is the absorbance ratio technique, …

In Vivo Evaluation The Efficiency Of Nitazoxanide With Cationic Gemini Surfactant On Cryptosporidiosis, Zeinab Ahmed, Eman M. Kandeel, Hagar F. Abdelmaksoud, Tarek Aboushousha, Entsar E. Badr

Baghdad Science Journal

Infection with cryptosporidiosis endangers the lives of many people with immunodeficiency, especially HIV patients. Nitazoxanide is one of the main therapeutic drugs used to treat cryptosporidiosis. However, it is poorly soluble in water, which restricts its usefulness and efficacy in immunocompromised patients. Surfactants have an amphiphilic character which indicates their ability to improve the water solubility of the hydrophobic drugs. Our research concerns the synthesis of new cationic Gemini surfactants that have the ability to improve the solubility of the drug Nanazoxide. So, we synthesized cationic Gemini surfactants. N1,N1,N3,N3-tetramethyl-N1,N3-bis(2-octadecanamidoethyl)propane-1,3-diaminium bromide (CGSPS18) and 2,2‘-(ethane-1,2-diylbis(oxy))bis(N-(2-octadecanamidoethyl)-N,N-dimethyl-2-oxoethane-1-aminium) dichloride (CGSES18) and the detection of their …

Sprouty4 Is Epigenetically Upregulated In Human Colorectal Cancer, Alexei J. Stuckel, Shuai Zeng, Zhen Lyu, Wei Zhang, Xu Zhang, Urszula Dougherty, Reba Mustafi, Tripti Khare, Qiong Zhang, Trupti Joshi, Marc Bissonnette, Sharad Khare

Biomedical Sciences

Sprouty4 (SPRY4) has been frequently reported as a tumor suppressor and is therefore downregulated in various cancers. For the first time, we report that SPRY4 is epigenetically upregulated in colorectal cancer (CRC). In this study, we explored DNA methylation and hydroxymethylation levels of SPRY4 in CRC cells and patient samples and correlated these findings with mRNA and protein expression levels. Three loci within the promoter region of SPRY4 were evaluated for 5mC levels in CRC using the combined bisulfite restriction analysis. In addition, hydroxymethylation levels within SPRY4 were measured in CRC patients. Lastly, DNA methylation and mRNA expression data were …

Electrodeposition Studies Of Gold Nanoparticles And Alginate Hydrogel Films., Harikrishnan Nambiathan Nambiar

Electronic Theses and Dissertations

This dissertation investigates the electrochemical deposition (ECD) and electrophoretic deposition (EPD) processes involving citrate (cit)-stabilized Au nanoparticles (NPs) and calcium alginate (Ca-Alg-Au NP) hydrogel films. The first part of the dissertation investigates the influence of NP size, surface area (SA) coverage, and ligand stabilizers on the electrochemical growth of Au on Au NPs, aiming to enhance our understanding of the growth process during the nucleation and growth of metal NPs. The growth kinetics depend on Au NP size (4 nm > 15 nm > 50 nm) for a constant SA coverage, as determined by the overpotentials for ECD. The growth rate increases …

Principals’ Leadership Of The Integration Of The Neurosequential Model In Education, Alison Grace Ostrom

Dissertations

The impact of childhood trauma on students in schools has become a relevant issue for today’s school leaders in the United States (Mendelson et al., 2015). The effects of trauma often interfere with students’ ability to learn and manage the demands of a school environment. Students are coming into schools with diverse academic, behavioral, and social-emotional needs, which has led school leaders to adopt trauma-informed approaches and programs in schools (Rossen, 2020). There are many trauma-informed programs available to school principals, one of them being the Neurosequential Model in Education (NME). This particular approach connects trauma-informed practices to neurobiological principles …

Student Desire To Stay In A Program: The Influence Of Faculty Caring And Cultural Identification With Faculty, Elizabeth Ann Cunniff

Dissertations

The Future of Nursing 2020-2030 report (National Academies of Sciences, Engineering, and Medicine, 2021) stated nurses should reflect the characteristics of the people they serve to ensure individuals receive culturally competent and equitable health care. Over the next decade and beyond, the U.S. population will become more racially and ethnically diverse, but the nursing workforce has not kept pace with the changing demographics (American Association of Colleges of Nursing, 2023). To increase diversity among nurses, nursing schools need more diverse graduates from prelicensure nursing programs, but attrition is greater among students with diverse racial, ethnic, and cultural backgrounds (Barbé et …

Cyclin D1 Extensively Reprograms Metabolism To Support Biosynthetic Pathways In Hepatocytes, Heng Wu, Betsy T. Kren, Andrew N. Lane, Teresa A. Cassel, Richard M. Higashi, Teresa Whei-Mei Fan, George S. Scaria, Laurie L. Shekels, Mark A. Klein, Jeffrey H. Albrecht

Markey Cancer Center Faculty Publications

Cell proliferation requires metabolic reprogramming to accommodate biosynthesis of new cell components, and similar alterations occur in cancer cells. However, the mechanisms linking the cell cycle machinery to metabolism are not well defined. Cyclin D1, along with its main partner cyclindependent kinase 4 (Cdk4), is a pivotal cell cycle regulator and driver oncogene that is overexpressed in many cancers. Here, we examine hepatocyte proliferation to define novel effects of cyclin D1 on biosynthetic metabolism. Metabolomic studies reveal that cyclin D1 broadly promotes biosynthetic pathways including glycolysis, the pentose phosphate pathway, and the purine and pyrimidine nucleotide synthesis in hepatocytes. Proteomic …

Magnetic Resonance Imaging Of Macrophage Response To Radiation Therapy, Harrison Yang, Brock Howerton, Logan Brown, Tadahide Izumi, Dennis C. Cheek, Jason Anthony Brandon, Francesc Marti, Roberto Gedaly, Reuben Adatorwovor, Fanny Chapelin

Markey Cancer Center Faculty Publications

Background: Magnetic resonance imaging (MRI) is a non-invasive imaging modality which, in conjunction with biopsies, provide a qualitative assessment of tumor response to treatment. Intravenous injection of contrast agents such as fluorine (19F) nanoemulsions labels systemic macrophages, which can, then, be tracked in real time with MRI. This method can provide quantifiable insights into the behavior of tumor-associated macrophages (TAMs) in the tumor microenvironment and macrophage recruitment during therapy.

Methods: Female mice received mammary fat pad injections of murine breast or colon cancer cell lines. The mice then received an intravenous 19F nanoemulsion injection, followed by a baseline 19F MRI. …

Md_Harmonize: A Python Package For Atom-Level Harmonization Of Public Metabolic Databases, Huan Jin, Hunter N. B. Moseley

Markey Cancer Center Faculty Publications

A major challenge to integrating public metabolic resources is the use of different nomenclatures by individual databases. This paper presents md_harmonize, an open-source Python package for harmonizing compounds and metabolic reactions across various metabolic databases. The md_harmonize package utilizes a neighborhood-specific graph coloring method for generating a unique identifier for each compound via atom identifiers based on a compound’s chemical structure. The resulting harmonized compounds and reactions can be used for various downstream analyses, including the construction of atom-resolved metabolic networks and models for metabolic flux analysis. Parts of the md_harmonize package have been optimized using a variety of computational …

Design And Characterization Of A Novel Eef2k Degrader With Potent Therapeutic Efficacy Against Triple-Negative Breast Cancer, Changxin Zhong, Rongfeng Zhu, Ting Jiang, Sheng Tian, Xiaobao Zhao, Xiaoya Wan, Shilong Jiang, Zonglin Chen, Rong Gong, Linhao He, Jin-Ming Yang, Na Ye, Yan Cheng

Markey Cancer Center Faculty Publications

Dysregulated eEF2K expression is implicated in the pathogenesis of many human cancers, including triple-negative breast cancer (TNBC), making it a plausible therapeutic target. However, specific eEF2K inhibitors with potent anti-cancer activity have not been available so far. Targeted protein degradation has emerged as a new strategy for drug discovery. In this study, a novel small molecule chemical is designed and synthesized, named as compound C1, which shows potent activity in degrading eEF2K. C1 selectively binds to F8, L10, R144, C146, E229, and Y236 of the eEF2K protein and promotes its proteasomal degradation by increasing the interaction between eEF2K and the …

A Multi-Faceted Approach To The Alzheimer’S Disease Proteome: From Human To Mouse Models, Jay M. Yarbro

Theses and Dissertations (ETD)

Amyloid pathology is the central hallmark of Alzheimer’s disease (AD) and related dementias (ADRD). We analyzed the dementia with Lewy bodies disease (DLBD) proteome, a disorder also associated with amyloid pathology, and compared to the AD proteome, identifying conserved alterations in amyloid pathology and shared components in amyloid plaques, including MDK and NTN1. We additionally evaluated major AD mouse models to determine their relevance to human disease and identify shared proteomic alterations. We observed alterations in several highly conserved matrisomal components consistently im- plicated in proteomic analyses of human amyloidosis disorders. We additionally identified several pathways influenced by amyloid-pathology that …

Matrix Gene Splicing And Emergence Of Highly Pathogenic Avian Influenza, Christopher Patton

Theses and Dissertations (ETD)

Highly pathogenic avian influenza (HPAI) is a continual threat to the commercial poultry industry. In the past 40 years there have been five outbreaks of HPAI in North America. Rapid responses to these outbreaks have been crucial in preventing HPAI from becoming endemic in North America. The three worst outbreaks of HPAI in North America occurred in 1983-84, 2014-15, and 2021-23. The most recent outbreak was the worst North American HPAI outbreak in recorded history and will likely result in endemicity. Here we described the three worst recorded HPAI outbreaks in North America. The first of these outbreaks occurred in …

Reducing Inputs And Adding Value To Turfgrass Systems Through Clover Inclusion And Plant Growth-Promoting Rhizobacteria Applications, Paige E. Boyle

All Graduate Theses and Dissertations, Fall 2023 to Present

Recently, the US has seen an expansion in the amount of turfgrass land cover (lawns, parks, roadsides, sports fields, and golf courses), as well as an interest in reducing fertilizer, water, and pesticide use in these grass systems. To help maintain quality and function while reducing resource inputs, two promising approaches have emerged: planting clover into lawns and applying plant growth-promoting rhizobacteria.

White clover and grass mixtures have been studied for their ability to cut down on fertilizer usage and provide a uniform, dark green lawn, but other clover types have not been as widely studied and may provide similar …

Biomarkers Of Objective Criteria For Subtle Cognitive Decline In Parkinson’S Disease, Mary Ellen Garcia

Electronic Theses, Projects, and Dissertations

Mild cognitive impairment in Parkinson’s disease (PD-MCI) is the continuum from normal cognitive function to dementia. Recent studies suggest that objectively defined subtle cognitive decline (Obj-SCD), which uses non-traditional “process” neuropsychological scores, may be a better pathway to earlier detection of cognitive impairment. Obj-SCD has been defined as the stage where cognition is not impaired, but biomarkers are present or cognitive impairment is minimal but not sufficient to meet MCI or dementia criteria. We examined the longitudinal trajectories of neurodegenerative markers among individuals who are classified as cognitive normal (CN), Obj-SCD, and PD-MCI. Past literature has been inconsistent about the …

Edaphic And Climatic Regulation Of Microbial Carbon-Use Efficiency In Managed Semi-Arid Systems, Kirsten R. Butcher

All Graduate Theses and Dissertations, Fall 2023 to Present

In agricultural systems, an increasingly prevalent goal is to promote carbon sequestration into stable, mineral-associated soil organic matter, as increases in soil organic matter have been linked to increases in soil water-holding capacity and increases in nutrient availability. Because microbially-produced compounds are the foundation of soil organic matter, understanding interactions between management strategies and abiotic controls on microbial activity can prove invaluable for producers and ranchers interested in building soil organic matter and safeguarding production systems under a changing climate. The ability of microorganisms to contribute to growing soil organic matter stocks is dictated by their carbon-use efficiency, which is …

Statistical Analysis Of Health Habits For Incoming College Students, Wendy Isamara Lizarraga Noriega

Electronic Theses, Projects, and Dissertations

Health habits among college students are commonly overseen, especially for students transitioning from high school right into college. These students are becoming independent young adults, and learning how to adapt to a different scenery when it comes to their learning environment. As these young adults transition into college, this is the perfect time for the students to become more vulnerable and comfortable with their independence, and their weight begins to fluctuate. Many variables come into consideration when increasing weight as an incoming first-year student. Students are more likely to live alone, get a job, and rely on fast food and …

A Mixed Methods Design Exploring Factors And Experiences Of Ed.D. Graduates And Time To Degree, Brian Willess

Electronic Theses, Projects, and Dissertations

In higher education, the extended time-to-degree of doctoral students creates a gap between those who graduate on time and those who do not. This delay is due to program structures, relationships, and dissertation writing. Academic success requires timely program completion and increases career opportunities for graduates. A long time to degree increases the risk of non-completion, especially during dissertations. Attrition, persistence, and dropping out have been studied, but why students graduate on time needs more examination. Most studies focus on Ph.D. students and programs with different requirements and time-to-degree, so new research must include Ed.D. students and examine doctoral graduates' …

General Population Projection Model With Census Population Data, Takenori Tsuruga

Electronic Theses, Projects, and Dissertations

The US Census Bureau offers a wide range of data, and within this array, the American Community Survey 5-Year Estimate (ACS5) serves as a valuable resource for understanding the US population. This project embarks on an exploration of Machine Learning and the Software Development process with the goal of generating effective population projections from ACS5 data. The project aims to provide methods to make predictions for every city and town in the US, encompassing their total population and population divided into 5-year age groups. It's worth noting that while the generation of these projections is grounded in the generalized statistical …

The Impact Of Covid-19 Pandemic On Fan Attendance In Major League Soccer (Mls) 2018-2022, Benjamin Appiah

Electronic Theses, Projects, and Dissertations

ABSTRACT

This culminating experience project investigates the impact of the COVID-19- on Major League Soccer (MLS). The research questions are: (Q1) How did the pandemic impact the trend of fan attendance in the MLS during the COVID-19 pandemic? (Q2) What factors impacted fan attendance in MLS games before the COVID-19 pandemic? (Q3) What factors impact fan attendance in MLS games after COVID-19 pandemic? The findings were: (Q1) Average annual fan attendance declined by 93.61% in 2019 and 2020, and an annua l increase of 49.47% between the year of 2020 and 2021. (Q2) The study revealed that 72.9% of the …