Intergenic Transcription In In Vivo Developed Bovine Oocytes And Pre-Implantation Embryos, Saurav Ranjitkar, Mohammad Shiri, Jiangwen Sun, Xiuchun Tian

Computer Science Faculty Publications

Background

Intergenic transcription, either failure to terminate at the transcription end site (TES), or transcription initiation at other intergenic regions, is present in cultured cells and enhanced in the presence of stressors such as viral infection. Transcription termination failure has not been characterized in natural biological samples such as pre-implantation embryos which express more than 10,000 genes and undergo drastic changes in DNA methylation.

Results

Using Automatic Readthrough Transcription Detection (ARTDeco) and data of in vivo developed bovine oocytes and embryos, we found abundant intergenic transcripts that we termed as read-outs (transcribed from 5 to 15 kb after TES) and …

Hashes Are Not Suitable To Verify Fixity Of The Public Archived Web, Mohamed Aturban, Martin Klein, Herbert Van De Sompel, Sawood Alam, Michael L. Nelson, Michele C. Weigle

Computer Science Faculty Publications

Web archives, such as the Internet Archive, preserve the web and allow access to prior states of web pages. We implicitly trust their versions of archived pages, but as their role moves from preserving curios of the past to facilitating present day adjudication, we are concerned with verifying the fixity of archived web pages, or mementos, to ensure they have always remained unaltered. A widely used technique in digital preservation to verify the fixity of an archived resource is to periodically compute a cryptographic hash value on a resource and then compare it with a previous hash value. If the …

Diversity Of The Gut Microbiome Of Chicken Fed With Black Soldier Fly Larvae -Based Feeds, Evalyne Wambui Ndotono

All Master’s Theses

Industrial rearing of insects, especially the black soldier fly, is gaining momentum in recent years because of the increase in food and feed insecurity, high prices of animal feeds and animal proteins, and population growth. This in turn has led to increased global demand for alternative sources of protein apart from traditional livestock products. This study focused on evaluating the gut microbial community dynamics of both the layer and broiler chickens that have been fed on BSF larvae-based diet. The bacterial communities were characterized using high throughput Oxford nanopore sequencing of the full-length bacterial 16S rRNA gene and downstream analysis …

Coming Of Age In The Eyes Of The Law: Theconflict Between Miranda, J.D.B., And Puberty, David M. N. Garavito

Articles

Everyone knows that going through puberty is associated with a multitude of changes: physical, mental, hormonal, etc. Fewer people know that when and how fast one goes through puberty can also be associated with changes to one’s legal rights. The Supreme Court of the United States held, in the landmark case of J.D.B. v. North Carolina, that there were many “commonsense conclusions” that could be drawn from how a child’s age would affect their interactions with law enforcement. In that case, the Court was deciding whether age should affect whether a child was considered “in custody” of the police, granting …

What Is Your Why? A Qualitative Study About Motivational Differences Of Latinos/Hispanics And African American/Black In A Computerized Cognitive Training Program To Prevent Alzheimer’S Disease, Lizbeth C. Vera Murillo

UNF Graduate Theses and Dissertations

Recruitment of diverse populations is a major barrier in advancing clinical research (Areán & Gallagher-Thompson, 1993; Areán & Alvidrez, 2003). The need to increase racial diversity is imperative due to the substantial growth of historically marginalized racial communities in the United States (Weinstein et al., 2017). Despite researchers’ efforts to increase racial/ethnic representation in clinical trials, there is still a lack of understanding of the best practices to recruit racial/ethnic minorities in clinical trials. The current study explores the qualitative motivations of why research participants (aged 65+ years old) volunteered for an Alzheimer’s Disease (AD) prevention clinical trial. Racial/ethnic differences …

In God's Hands: Faith Healing, Epilepsy, And The Question Of Human Rights, Marisa L. Pechillo

UNF Graduate Theses and Dissertations

This thesis highlights the historical link between people with epilepsy (PWE) and demonic possession. The discussion traces origins of these views from antiquity to the contemporary era, foregrounding media and scholarship that emphasizes the negative perceptions of PWE developed through Christian teaching and imagery. It explores the question of whether the social isolation, abuse, and violence committed against PWE, through Christian faith healing practices such as exorcism, developed in relation to these stigmatizing views and whether these constitute a human rights violation in accordance with conventions put forth by the United Nations.

Homecoming: Examining Built Relationships That Influence Historically Black College And University Student Experiences, Alvenia Effie Derban

UNF Graduate Theses and Dissertations

Maintaining and increasing student retention is a challenge for small institutions especially small private Historically Black Colleges and Universities (HBCU). There are factors which contribute to this problem of practice and solutions that could increase student retention given more research and analysis. The purpose of this instrumental case study was to explore and examine the campus professional-student relationships to better understand the first-generation/first-time college student’s experience and how these relationships may influence their retention. This study examined the experiences of three Edward Waters University students to understand a problem of practice experienced at Edward Waters University and many others, student …

Physiological Responses Regarding Stress And Trauma: The Freeze Response, Emma Arie Von Holten

UNF Graduate Theses and Dissertations

Fight-or-flight is a commonly known response to threatening situations, but the freeze response is another defensive mechanism that might arise in such situations. There is also evidence that exposure to trauma can impact physiological reactions when defensive mechanisms are not required, such as freezing in response to non-threatening situations. The current study aimed to replicate and expand upon a limited number of previous studies on the human freeze response by implementing measurements of postural sway, heart rate, and infrared thermography while valenced images were shown to participants. Ninety-two participants were recruited from the University of North Florida and participants were …

Shaping Teachers’ Behavioral Intentions To Use Dgbl: Understanding The Key Insights Teachers Garner From A Research-Based Dgbl Professional Development Design, Nadine Ebri

UNF Graduate Theses and Dissertations

This study aims to explore the key insights teachers garner from a research-based Digital Game-Based Learning (DGBL) professional development (PD) workshop and how their garnered insights shape their behavioral intentions to use DGBL in their future teaching practices. By gaining a deeper understanding of these areas, this study aims to enhance the effectiveness of professional development workshops for DGBL, and in turn, improve DGBL implementation in the classroom setting.

DGBL, which integrates digital games into education, has gained attention for its potential to engage and motivate students while enhancing learning outcomes. However, limited focus on DGBL professional development …

Field Assessment Of The Pervious Oyster Shell Habitat: Enhancement Of Intertidal Habitat For The Eastern Oyster And Associated Nekton In Northeast Florida, Hunter Mathews

UNF Graduate Theses and Dissertations

A dramatic global decline in oyster reef habitat has led to widespread deployment of oyster restoration projects. Efforts are underway to move away from polluting methods like plastic mesh bags in oyster deployments. Additionally, increasing pressure on our estuaries from recreational boat wakes and intensified storms requires more robust structures for shoreline protection. The “Pervious Oyster Shell Habitat” (POSH) is a novel artificial reef structure composed of oyster shell bound by a thin layer of Portland cement, into the shape of a dome. The structure’s makeup greatly reduces its environmental impact, while providing quality substrate for provision of oyster reef …

Mouthwashes: Alternatives And Future Directions, Brett Duane, Tami Yap, Prasanna Neelakantan, Robert Anthonappa, Raul Bescos, Colman Mcgrath, Michael Mccullough, Zoë Brookes

Peninsula Dental School

This narrative review summarises “alternative” or “natural” over-the-counter (OTC) mouthwashes not covered elsewhere in this supplement and newly emerging products, as potential mouthwashes of the future. The “natural” mouthwashes reviewed include saltwater, baking soda, coconut oil, charcoal, propolis, seaweeds, and probiotics. Other than essential oils, it is apparent that their clinical effectiveness is still under debate, but there is some evidence to suggest that propolis reduces plaque and gingivitis. This review also covers the host immune response, via novel anti-inmmunomodulant mouthwashes, such as erythropoietin to reduce inflammation with oral mucositis (OM) after radiotherapy. The emerging concept of nanoparticle-containing mouthwashes, such …

Antimicrobial Mouthwashes: An Overview Of Mechanisms—What Do We Still Need To Know?, Zoë Brookes, Colman Mcgrath, Michael Mccullough

Peninsula Dental School

This narrative literature review is the first in a 6-section supplement on the role of mouthwashes in oral care. This introduction briefly summarises current knowledge on antimicrobial mechanisms, relating to some of the most common over-the-counter mouthwash products available worldwide: chlorhexidine, hydrogen peroxide, cetylpyridinium chloride, povidone iodine, and essential oils. The aim of this first article is to describe how mouthwashes “kill” pathogenic microbes when used adjunctively and thus provide a basis for their widespread use to manage key oral diseases, namely caries, gingivitis, and periodontal disease. This article therefore sets the scene for subsequent, more detailed exploration of mouthwashes …

Developmental Pharmacokinetics Of Indomethacin In Preterm Neonates: Severely Decreased Drug Clearance In The First Week Of Life., Wojciech Krzyzanski, Bradley Stockard, Andrea Gaedigk, Allison Scott, Whitney M. Nolte, Kim T. Gibson, J Steven Leeder, Tamorah Lewis

Manuscripts, Articles, Book Chapters and Other Papers

Indomethacin is used commonly in preterm neonates for the prevention of intracranial hemorrhage and closure of an abnormally open cardiac vessel. Due to biomedical advances, the infants who receive this drug in the neonatal intensive care unit setting have become younger, smaller, and less mature (more preterm) at the time of treatment. To develop a pharmacokinetics (PK) model to aid future dosing, we designed a prospective cohort study to characterize indomethacin PK in a dynamically changing patient population. A population PK base model was created using NONMEM, and a covariate model was developed in a primary development cohort and subsequently …

Preclinical Efficacy Of Azacitidine And Venetoclax For Infant Kmt2a-Rearranged Acute Lymphoblastic Leukemia Reveals A New Therapeutic Strategy., Laurence C. Cheung, Carlos Aya-Bonilla, Mark N. Cruickshank, Sung K. Chiu, Vincent Kuek, Denise Anderson, Grace-Alyssa Chua, Sajla Singh, Joyce Oommen, Emanuela Ferrari, Anastasia M. Hughes, Jette Ford, Elena Kunold, Maria C. Hesselman, Frederik Post, Kelly E. Faulk, Erin H. Breese, Erin M. Guest, Patrick A. Brown, Mignon L. Loh, Richard B. Lock, Ursula R. Kees, Rozbeh Jafari, Sébastien Malinge, Rishi S. Kotecha

Manuscripts, Articles, Book Chapters and Other Papers

Infants with KMT2A-rearranged B-cell acute lymphoblastic leukemia (ALL) have a dismal prognosis. Survival outcomes have remained static in recent decades despite treatment intensification and novel therapies are urgently required. KMT2A-rearranged infant ALL cells are characterized by an abundance of promoter hypermethylation and exhibit high BCL-2 expression, highlighting potential for therapeutic targeting. Here, we show that hypomethylating agents exhibit in vitro additivity when combined with most conventional chemotherapeutic agents. However, in a subset of samples an antagonistic effect was seen between several agents. This was most evident when hypomethylating agents were combined with methotrexate, with upregulation of ATP-binding cassette transporters identified …

Adverse Maternal Environment Alters Oprl1 Variant Expression In Mouse Hippocampus., Xingrao Ke, Yingliu Huang, Qi Fu, Amber Majnik, Venkatesh Sampath, Robert H. Lane

Manuscripts, Articles, Book Chapters and Other Papers

An adverse maternal environment (AME) and Western diet (WD) in early life predispose offspring toward cognitive impairment in humans and mice. Cognitive impairment associates with hippocampal dysfunction. An important regulator of hippocampal function is the hippocampal Nociceptin/Orphanin FQ (N/OFQ) system. Previous studies find links between dysregulation of hippocampal N/OFQ receptor (NOP) expression and impaired cognitive function. NOP is encoded by the opioid receptor-like 1 (Oprl1) gene that contains multiple mRNA variants and isoforms. Regulation of Oprl1 expression includes histone modifications within the promoter. We tested the hypothesis that an AME and a postweaning WD increase the expression of hippocampal Oprl1 …

How Certain Robot Attributes Influence Human-To-Robot Social And Emotional Bonds, James Gaskin, P. Thomas Fife, Warren Rosengren

Faculty Publications

A growing population of humans are feeling lonely and isolated and may therefore benefit from social and emotional companionship. However, other humans cannot always be available to fulfill these needs, and such in-need individuals often cannot care for pets. Therefore, we explore how robot companions may be designed to facilitate bonds with humans. Our preliminary examination of 115 participants in a scenario based quasi-experimental study suggests that humans are more likely to develop social and emotional bonds with robots when those robots are good at communicating and conveying emotions. However, robots’ anthropomorphic attributes and responsiveness to external cues were found …

Worth Writing! Worth Reading! Edition Viii (2020 - 2023), New York Law School

New York Law School Community Newsletter

No abstract provided.

An Examination Of A Traditional Business Curriculum And Its Effect On Students’ Level Of Moral Decision Making, Brian M. Rothschild

Theses and Dissertations

The questionable decisions of prominent business leaders in major industries in the last two or more decades has called into question the ethical training corporate leaders receive and their ethical decision-making abilities. The research has been extensive but inconclusive as to whether a traditional business curriculum has the effect of developing the moral decision-making abilities of business majors at universities who may end up in industry leadership positions. Therefore, this applied dissertation was designed to contribute to the literature by providing additional information regarding using ethics as a theme throughout a traditional undergraduate business curriculum.

The study included 141 freshman …

When The Math Matters: Use Of P-Values In Pharmaceutical Litigation, Robin L. Juni

GW Law Faculty Publications & Other Works

This article explains the use of p-values as part of statistical analyses to support several types of pharmaceutical litigation, focusing on how lawyers can best present relevant studies to benefit their clients “when the math matters” and indeed critically affects the outcome of the dispute. The article begins with a conceptual discussion of p-values as utilized in the scientific community and continues by describing the substantial concerns many scientists have voiced about the use of p-values to show much of anything. The article then explains use by lawyers and judges of p-values in litigation, particularly three aspects of pharmaceutical litigation, …

Amending The Defense Production Act: Preventing Another Pandemic, Madalyn Mcgunagle

Roger Williams University Law Review

No abstract provided.

Lacera V. Department Of Children, Youth, And Families, 272 A.3d 1064 (R.I. 2022), Hilary L. Levey Friedman

Roger Williams University Law Review

No abstract provided.

Investigation Of Relationships Between Serum Lactate, Acute Phase Proteins, Pro/ Antiinflammatory Cytokine Levels, And Metritis Formation In Holstein Dairy Heifers, Talha Avcilar, Davut Koca, Yavuz Nak, Mustafa Eren Şahi̇n, Musa Özgür Özyi̇ği̇t, Deni̇z Nak

Turkish Journal of Veterinary & Animal Sciences

The main purpose of this investigation was to identify and suggest suitable early screening inflammatory markers for the early diagnosis of uterine infections such as acute septic metritis (ASM) in the Holstein heifers. In addition, to achieve the above -mentioned aim, the objectives of the present study were tried to determine the relationship between blood serum lactate, acute phase proteins (APPs), pro/anti inflammatory cytokine levels, and metritis formation in Holstein heifers. For this purpose, 250 Holstein heifers which were 11-13 months old, were chosen as the study material. After heifers were conceived, blood serum samples were collected at the 8th …

Effect Of Different Light Intensities And Colors On Growth Performance And Reproductive Characteristics In Japanese Quails, Fawwad Ahmad, Muhammad Sharif, Muhammad Ashraf, Muhammad Riaz, Muhammad Shoaib, Talha Siddique, Aqian Ahmed, Ahmad Hamad Sheir, Muhammad Ahsan Yaseen, Muhammad Mahboob Ali Hamid

Turkish Journal of Veterinary & Animal Sciences

Light is one of the most important environmental factors in quail farming, as it has a significant impact on production efficiency and reproductive characteristics. The purpose of this study was to determine the effect of different light intensities and colors on production performance in Japanese quails. For the first experiment, 200 Japanese quails were divided into five treatment groups, each with four replicates (10 birds in each). The birds in the control treatment were exposed to a light intensity of 20 lux, while the other four treatment groups were exposed to light intensities of 10 lux, 15 lux, 25 lux, …

Arterial Vascularization Of The Spleen In Merino Sheep, Melek Tosun, Şükrü Hakan Atalgin

Turkish Journal of Veterinary & Animal Sciences

In this study, it is aimed to reach detailed information about the anatomy of arteries in the sheep spleen, which can be used as an example and model for the success of planned or emergency surgical interventions related to the spleen. In spleen injuries, the spleen may be divided into two or three parts, or it may be limited to cracks on the spleen. In particular, it is aimed to protect the organs that have been damaged by trauma recently. Operations such as splenectomy and splenography are surgical procedures that require good knowledge of the pathways of artery and its …

Modeling Of Individual Egg Weights Of Lohmann-Brown Layer Hens, Esra Yavuz, Samet Hasan Abaci, Kadi̇r Erensoy, Mustafa Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the most suitable model for egg weights of the Cubic, Gompertz, Logistics, Gamma, Richard, Piecewise Quadratic, Orscov, and Sigmoaidal models, which are widely used in estimation. Lohmann-Brown Classic laying hens raised in Ondokuz Mayıs University Research and Application farm were used as animal material. In the modeling of egg weights of 351 layer hens raised in 3-storey cages, individual egg weight data measured at weekly intervals, 32 on the 1st floor, 17 on the 2nd floor, and 27 on the 3rd floor, were taken into account, and a total of 76 hens' individual …

The Effect Of Simvastatin Treatment On Tnf-Alpha-Induced Inflammation In Human Vascular Endothelial Cells (Huvecs), Büşra Görgün, Betül İşi̇ner Kaya, Kübra Kaya, Leyla Di̇dem Kozaci

Turkish Journal of Veterinary & Animal Sciences

This study investigates the effects of simvastatin (SMV) on human umbilical vein endothelial cells (HUVECs) inflamed by tumor necrosis factor-alpha (TNF-a). Cells were treated with simvastatin (5 μM, 10 μM, and 50 μM) with/without TNF (20 ng/mL). The effects of simvastatin on the proliferation of cells were examined, and its cytotoxic effect was determined by lactate dehydrogenase (LDH) assays. Metalloproteinase expression (MMP-2 and MMP-9) levels of SMV at different concentrations were determined by qPCR, and protein levels were determined by Western blot. The increasing concentrations of SMV down-regulated the expression levels of MMPs. After stimulation with TNF-a, the effect of …

The First Record Of Philophthalmus Lucipetus (Trematoda: Philophthalmidae) In The Great Egret (Ardea Alba) From Turkey, Mehmet Öztürk, Şi̇nasi̇ Umur

Turkish Journal of Veterinary & Animal Sciences

The genus Philophthalmus, Looss 1899 (Echinostoma: Philophthalmidae) is a cosmopolitan group of parasites that infects the eyes of birds and mammals, including humans. A great egret (Ardea alba) was found dead in Kızılırmak Delta, Samsun, Turkey. The bird was transported to the Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun Turkey, for a necropsy. In the parasitological examination, a trematode was detected in the eye, and then it was identified as Philophthalmus lucipetus. This is the first report of the zoonotic parasite P. lucipetus in Turkey.

Investigation Of The In Vitro Antioxidant Activity Of Fermented Tomato Pruning Residues And Its Effects On Biochemical And Hematological Parameters Of Rats, Bariş Denk, Beyza Aydoğan, İsmai̇l Bayram, Abdurrahman Fati̇h Fi̇dan

Turkish Journal of Veterinary & Animal Sciences

In the tomato greenhouse production process, a by-product emerges in the form of greenhouse residues. One of these residues is leaf pruning residue, which poses an environmental pollution problem in terms of waste management. The purpose of this work was to investigate the use of fermented tomato leaves (FTL) as an animal feed additive and its potential to mitigate the environmental pollution problem caused by greenhouse pruning residues. The pruning residues were fermented and their in vitro antioxidant activity (AOA) levels were analyzed using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH), reducing power (RP), and total phenolic content (TPC) assays. Wistar albino …

Investigation Of Bmp15 And Gdf9 Gene Polymorphisms And Their Effects On Litter Size In Anatolian Sheep Breed Akkaraman, Koray Kirikçi

Turkish Journal of Veterinary & Animal Sciences

This study aimed to examine the polymorphism of the BMP15 (FecX$^{G}$ and FecX$^{I}$) and GDF9 (G1 and G4) genes and the association between genotypes and litter size in the Akkaraman breed. Blood samples were taken from 100 ewes in total. Genomic DNA was obtained from whole blood. The PCR-RFLP technique was employed to determine the polymorphisms of the genes under investigation. The G1 was the only polymorphic among the four loci analyzed. The frequencies for the GA and GG genotypes were 0.26 and 0.74, respectively, while the A and G allele frequencies were 0.13 and 0.87, respectively. The observed and …

Investigation Of Snps In Bmp15 And Gdf9 Genes In "Çepni" And "Of" Sheep In The Black Sea Region Of Turkey, Koray Kirikçi

Turkish Journal of Veterinary & Animal Sciences

Sheep fertility genes have been studied in Turkey for many years. But so far it cannot be said that a satisfactory result has been achieved in terms of the number of detected polymorphisms on fertility genes. Exhibiting the genetic structure of local gene resources adapted to a particular region is very important for sustainability, conservation and breeding studies, and many other issues such as profitability. Therefore, the primary aim of this study was to investigate the single nucleotide polymorphisms (SNPs) on the BMP15 and GDF9 genes in two new Turkish sheep breeds, Çepni and Of. DNA sequencing was performed to …