Artificial Light At Night Disrupts Pain Behavior And Cerebrovascular Structure In Mice, Jacob Raymond Bumgarner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Artificial Light at Night Disrupts Pain Behavior and Cerebrovascular Structure in Mice

Jacob R. Bumgarner

Circadian rhythms are intrinsic biological processes that fluctuate in function with a period of approximately 24 hours. These rhythms are precisely synchronized to the 24- hour day of the Earth by external rhythmic signaling cues. Solar light-dark cycles are the most potent environmental signaling cue for terrestrial organisms to align internal rhythms with the external day. Proper alignment and synchrony of internal circadian rhythms with external environmental rhythms are essential for health and optimal biological function.

The modern human environment on Earth is no longer …

Meiotic Recombination In Zea Mays And Its Contribution To Genetic Diversity, Jasmine Elizabeth Freeman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Meiotic recombination plays a vital role in shaping genome evolution by introducing genetic diversity necessary for natural selection. Recombination rates exhibit extensive variation within and between species, occurring in specific regions known as hotspots and coldspots along individual chromosomes. Maize, with its well-characterized genome, provides an excellent model for studying the nonrandom pattern of meiotic recombination. While retrotransposon activity contributes to significant intergenic variation in maize inbred lines, the genic space remains conserved in terms of gene order and sequence. To investigate recombination activity in the Bz1/Sh1 region, the maize haplotypes W22 and B73, which share genetic backgrounds but differ …

3d Printed Microfluidic Devices For Advanced Fluid Manipulation In Biomedical Applications, Kathrine Curtin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microfluidics are valuable devices in the biomedical field because of their remarkable ability to manipulate small volumes of fluid and facilitate reactions. Microfluidics have several significant advantages, including their portability, low cost, fast reaction times, and high surface area-to-volume ratios. Microfluidics be used for a multitude of applications, including bioanalysis, cellular assays, organ-on-a-chip, and separations. Microfluidics have great potential to overcome many challenges in conventional chemical and biological applications. However, complex fabrication procedures in silicon, glass, thermoplastics, and (polydimethylsiloxane) PDMS devices make rapidly optimizing and prototyping devices challenging. Microfluidic fabrication techniques have been a long-standing challenge in successfully translating these …

Localized Delivery Of Gm-Csf In The Regulation Of Breast Tumor Oxygen And Anti-Tumor Immunity Through Macrophages, Nicole E. Mihalik

Graduate Theses, Dissertations, and Problem Reports (ETD)

GM-CSF has been employed as an adjuvant to cancer immunotherapies in solid tumors due to its ability to drive immune stimulatory macrophages and other myeloid cells that promote anti-tumor responses. However, GM-CSF does not always exert immune stimulatory effects on myeloid cells, and sometimes even drives immune suppressive phenotypes. GM-CSF effects on myeloid cells and the tumor microenvironment (TME) appear to vary across dosages and even cancer types. However, an optimal dosing regimen for GM-CSF has yet to be determined to-date, and this is further complicated by the fact that GM-CSF is often administered via oncolytic viruses or tumor vaccines …

Investigating The Effects Of Flotation Restricted Environment Stimulation Therapy On Neural Networks In Chronic Pain Patients Via Functional Magnetic Resonance Imaging And Other Modalities, Tyler Austin Mcgaughey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chronic lower back pain (CLBP) is one of the leading causes of disability worldwide, with an estimated direct cost of over 600 billion dollars in the US alone. CLBP profoundly impacts the patient, their support team, and the healthcare system. Widespread changes to the patient's well-being, including mental health, are seen in CLBP and contribute to a downward spiral that often leads to disability. Chronic pain is poorly understood and is difficult to treat because of its subjective nature. Recent improvements in pain assessment in the clinic and in biomedical research have had a profoundly impacted the field. More than …

Elucidating Mechanisms Of Proteasome Modulation By Small Molecules To Treat Neurodegenerative Diseases And Cancer, Janelle Joo Yee Chuah

Graduate Theses, Dissertations, and Problem Reports (ETD)

Maintaining protein homeostasis, or proteostasis, is crucial for cellular function, and protein degradation through the proteasome plays a pivotal role in this process. Dysregulation of the proteasome can lead to proteostasis collapse and has been linked to the development of neurodegenerative diseases and cancers. Neurodegenerative diseases often exhibit proteasome impairment, whereas proteasome upregulation is frequently observed in cancer. Although the use of proteasome inhibitors as a pharmaceutical approach has shown success in certain cancers, it has yet to demonstrate efficacy in neurodegenerative diseases due to the absence of drug-like small molecules capable of effectively reversing proteasome impairment and/or activating the …

Machine Learning For Biosensors, Gayathri Anapanani

Graduate Theses, Dissertations, and Problem Reports (ETD)

Biosensors have become increasingly popular as diagnostic tools due to their ability to detect and quantify biological analytes in a wide range of applications. With the growing demand for faster and more reliable biosensing devices, machine learning has become a valuable tool in enhancing biosensor performance. In this report, we review recent progress in the application of machine learning to biosensors. We discuss the potential benefits of using machine learning in biosensors, including improved sensitivity, selectivity, and accuracy. We also discuss the various machine learning techniques that have been applied to biosensors, including data preprocessing, feature extraction, and classification and …

Spatial Processing Of Conspecific Signals In Weakly Electric Fish: From Sensory Image To Neural Population Coding, Oak Everette Milam

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this dissertation, I examine how an animal’s nervous system encodes spatially realistic conspecific signals in their environment and how the encoding mechanisms support behavioral sensitivity. I begin by modeling changes in the electrosensory signals exchanged by weakly electric fish in a social context. During this behavior, I estimate how the spatial structure of conspecific stimuli influences sensory responses at the electroreceptive periphery. I then quantify how space is represented in the hindbrain, specifically in the primary sensory area called the electrosensory lateral line lobe. I show that behavioral sensitivity is influenced by the heterogeneous properties of the pyramidal cell …

Missile Modeling And Simulation Of Nominal And Abnormal Scenarios Resulting From External Damage, James Manuel Floyd Iii

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis presents the development of a six-degree-of-freedom flight simulation environment for missiles and the application thereof to investigate the flight performance of missiles when exposed to external damage. The simulation environment was designed to provide a realistic representation of missile flight dynamics including aerodynamic effects, flight control systems, and self-guidance. The simulation environment was designed to be modular, expandable, and include realistic models of external damage to the missile body obtained by adversarial counteraction.

The primary objective of this research was to examine missile flight performance when subjected to unspecified external damage, including changes in trajectory, stability, and controllability, …

Developing New Ionization Strategies For Characterizing Structural Flexibility, Heterogeneity, And Transformation Of Biopolymers, Daud Sharif

Graduate Theses, Dissertations, and Problem Reports (ETD)

Biopolymers, such as proteins and nucleic acids, serve important functions dictated by their structural folding. Information excavated from the intricate structural arrangements of biopolymers have been proved to be vital in diagnosing diseases, unveiling disease mechanisms, and developing new drugs. A continuous effort has been put to improve the characterization techniques of these biomolecules so that more precision can be achieved in terms of detailing their structure-function relationship. Mass spectrometry (MS) is one such technique that has become very relevant for structural biology with the emergence of soft ionization techniques such as electrospray ionization (ESI). A prerequisite to the application …

Associations Among Executive Functioning, Family Functioning, Adolescent Responsibility, And Adherence In Pediatric Inflammatory Bowel Disease, Cecily Newel Conour

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Inflammatory Bowel Disease (IBD) is a common cause of chronic pain for adolescents in the United States. Adherence to the treatment regimen is a significant concern, particularly for adolescents. Barriers to adherence are varied, but include cognitive factors, such as forgetting. Parent involvement is associated with increased adherence in this population, though adolescent involvement is less studied. Family functioning is associated with adherence to medication regimen across pediatric chronic illnesses, including IBD treatment regimen. To better inform clinical care, this study aims to understand the relations among adolescent’s responsibility, executive functioning, family functioning, and adherence. Methods: The current study …

Expansion On The Use Of The Novel Compound, Elp-004, Using A Mycobacterium Tuberculosis Experimentation Model, Kendyl Marie Berry

Graduate Theses, Dissertations, and Problem Reports (ETD)

Osteoarticular tuberculosis (OAT) is a bone degenerative disease that results in bone erosion, joint effusion, joint swelling, and sometimes, nerve compression. OAT occurs from the hematogenous dissemination of Mycobacterium tuberculosis (Mtb) bacilli spreading from the primary site of infection to the bones and joints; the primary site of infection tends to be the lungs as pulmonary tuberculosis infections are the most common. OAT can occur as the result of an active or latent infection. Latent infections result in OAT when bacilli are able to escape granulomas. The incidence of OAT is not as high as pulmonary infections, making up about …

Analysis Of ‘Touch’ Dna Recovered From Metal Substrates: An Investigation Into Cfdna-Metal Interactions And The Efficacy Of Different Collection Techniques On Dna Yield, Jessica E. Thornton

Graduate Theses, Dissertations, and Problem Reports (ETD)

While several improvements have been made in recent years to optimize the recovery of ‘touch’ DNA, relatively little research has been conducted to understand the relationship between ‘touch’ DNA and the binding affinity of that DNA to metal surfaces, specifically those with a significant copper presence. Furthermore, characterization of cell-free DNA (cfDNA) and its contribution to ‘touch’ samples and those cfDNA-metal interactions from objects commonly identified at crime scenes (cartridge casings, knives, doorknobs) have been lacking. Research has identified the tendency of copper ions to intercalate with DNA helices, resulting in sample degradation among other damaging conformational changes; however, while …

Optimal Path Planning For Aerial Robots Using Genetic Algorithm, Anna Puigvert I Juan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis presents a path optimization solution for a robot in two different constrained 3-dimensional (3D) environments. The robot is required to travel from its current position to a goal position following minimum cost paths (optimal paths). The first environment has 3D obstacles that interfere with the robot’s path. The path cost for this environment accounts for the minimum distance traveled by the robot from the start to the goal position while avoiding obstacles. The second environment is the atmosphere of Venus, specifically a flyable region of this atmosphere with characteristics similar to Earth’s. This environment has strong westward winds …

Computational Mechanisms Of Face Perception, Jinge Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

The intertwined history of artificial intelligence and neuroscience has significantly impacted their development, with AI arising from and evolving alongside neuroscience. The remarkable performance of deep learning has inspired neuroscientists to investigate and utilize artificial neural networks as computational models to address biological issues. Studying the brain and its operational mechanisms can greatly enhance our understanding of neural networks, which has crucial implications for developing efficient AI algorithms. Many of the advanced perceptual and cognitive skills of biological systems are now possible to achieve through artificial intelligence systems, which is transforming our knowledge of brain function. Thus, the need for …

Structural Health Monitoring Using Machine Learning And Synthetic Data, Michail Tzimas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Structural health monitoring spans many decades of research across multiple engineering fields. However, typical monitoring processes for damage detection of complex structures usually prohibit real-time or fast detection of debilitating damage to the structure. One of the major issues of real-time detection of damage is the enormity of data that needs to be processed, which is worsened by the relative inability of fast relaying of data to structural engineers. With the rapid advancement of Machine Learning, both issues can be overcome, and detection of failure is achieved with non-invasive techniques. This dissertation explores the applicability of Machine Learning as a …

A Comparison Of The Failure Rate Of Two Orthodontic Light-Curable Adhesives: A Chart Review, Ian Montgomery Weaver

Graduate Theses, Dissertations, and Problem Reports (ETD)

A Comparison of the Failure Rate of Two Orthodontic Light-Curable Adhesives: A Chart Review

Ian Montgomery Weaver, D.D.S.

Background and Objectives: The overhead costs of running a private orthodontic practice are infamously high, thus there is a notable benefit to finding the most cost effective methods of treating patients while still maintaining high quality care. A relatively new light-cure orthodontic adhesive BracePaste®^® (American Orthodontics™, Sheboygan, WI), introduced to the market in 2017, can fluoresce under UV light to assist in identifying the difference between the enamel of the tooth and the adhesive material itself, an ability which can aid …

Weigh-In-Motion Data-Driven Pavement Performance Prediction Models, Mohhammad Afsar Sujon

Graduate Theses, Dissertations, and Problem Reports (ETD)

The effective functioning of pavements as a critical component of the transportation system necessitates the implementation of ongoing maintenance programs to safeguard this significant and valuable infrastructure and guarantee its optimal performance. The maintenance, rehabilitation, and reconstruction (MRR) program of the pavement structure is dependent on a multidimensional decision-making process, which considers the existing pavement structural condition and the anticipated future performance. Pavement Performance Prediction Models (PPPMs) have become indispensable tools for the efficient implementation of the MRR program and the minimization of associated costs by providing precise predictions of distress and roughness based on inventory and monitoring data concerning …

Influence Of Maternal Nano-Titanium Dioxide Inhalation Exposure On Cyclooxygenase Metabolites: Maternal Microvascular And Sexually Dimorphic Feto-Placental Outcomes., Julie Anne Griffith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nano-titanium dioxide (nano-TiO₂) is an engineered nanomaterial (ENM), which is widely utilized in diverse products like surface coatings, cosmetics, medical devices, and filters. Inhaled nano-TiO₂ has been demonstrated to induce pulmonary inflammation, impair microvascular function, and hinder generational reproductive endpoints. These outcomes indicate why it is important to explore gestation, as it requires timely vascular adaptations to support maternal and fetal health and development. Therefore, the central hypothesis of my dissertation is: maternal nano-TiO₂ inhalation exposure will impact maternal microvascular function and fetoplacental hemodynamics and function in a sexually dimorphic manner.

The first purpose of …

A Case Study Of Makerere University In Uganda Through The Lens Of U.S. Land-Grant Higher Education, Christina L. Hand

Graduate Theses, Dissertations, and Problem Reports (ETD)

This case study examines Makerere University through the lens of U.S. land-grant ideal and normative domains in order to provide a deeper understanding of global higher education. A case study uses multiple types of data to create a holistic perspective. As well as interviewing Makerere stakeholders representing diverse sectors, numerous documents and different types of media were analyzed enabling a triangulation of data. Six major themes emerged focusing on Makerere’s aspiration to be a research-led institution, the impact of neoliberalism, challenges in undergraduate education, the importance of reputation and saga, the ever-present role of the Ugandan government, and the continuing …

Lxs Caravanerxs And Nonsecular Protest: Rethinking Migrant Family Separation With Un Llanto Colectivo, Marcelle Maese

English: Faculty Scholarship

Written and directed by Celia Herrera Rodríguez and Cherríe Moraga in 2018, "Un Llanto Colectivo: Press Conference & Performa-Protesta" took place in front of ICE headquarters in downtown San Diego (September 15th) and then in front of Otay Mesa Detention Center (September 16th). Envisioned as an act of consciousness raising around family separation, Central American caravans, and the detention-industrial-complex, the flyer invitation read, "[T]his is ceremonial resistance." This writing is an extension of my witness of and participation in Un llanto colectivo. I provide a historical context for lxs caravanerxs, as well as interviews with Maestras Celia and Cherríe. The …

Learning And Planning For Autonomous Systems With Emergent Hierarchical Representations And Decaying Short-Term Memory, Piotr Aleksander Bogdan

School of Engineering, Computing and Mathematics Theses

How do we create machines with the ability to capture, record and recall memories of past experience? How should these machines choose the most optimal action based on those stored memories? These seem like crucial questions for creating intelligent machines capable of learning from experience. The field of Artificial Intelligence (AI) is trying to reproduce such capabilities with increasing success. Currently a large portion of AI algorithms are focusing on making decisions based on big sets of learned past experience examples in the form of instantaneous input-output mapping. They operate as discrete models where time is collapsed into independent signal …

Graphene-Based Biosensors For Quantitative Characterisation Of Dna Methylation, Mina Safarzadeh

School of Engineering, Computing and Mathematics Theses

DNA methylation is associated with various diseases including cancer, cardiovascular diseases and neurodegenerative disorders, making it a potential biomarker to diagnose and prognose these diseases. Hence, a tool to detect and quantify DNA methylation can be valuable to improve clinical tests. In this thesis, we report two different biosensors for the detection and quantification of methylated tumour suppressor gene, O-6-methylguanine-DNA methyltransferase (MGMT), which is a potential biomarker for brain tumours and breast cancer. Both of the reported biosensors in this thesis are based on screen-printed reduced graphene oxide electrodes (rGO SPEs) and are electrochemical biosensors. In the Ab/ssDNA biosensor the …

Flaws In The Methodologies For Organic Carbon Analysis In Seagrass Blue Carbon Soils, Oscar Serrano, Ines Mazarrasa, James W. Fourqurean, Eduard Serrano, Jeffrey Baldock, Jonathan Sanderman

Research outputs 2022 to 2026

The ability to accurately measure organic carbon (OC) in marine sediments or soils is overall taken for granted in scientific communities, yet this seemingly mundane task remains a methodological challenge when the soil matrix contains calcium carbonate (CaCO3), creating inaccuracies in Blue Carbon estimates. Here, we compared five common methods combining acidification, combustion, and wet oxidation pre-treatments for determination of OC in sediments and soils containing CaCO3 based on the analyses of artificial soil mixtures made of different OC and CaCO3 contents, and multiple soils from Australian seagrass cores. The results obtained showed that methods involving acidification pre-treatment entailed −17 …

Investigating The Perspectives Of Older Adults In Residential Aged Care On Oral Health-Related Quality Of Life, Parisa Malekpour, Amanda Devine, Julie Dare, Leesa Costello

Research outputs 2022 to 2026

Objective

The objective of the study was to explore how older people living in a residential aged care facility perceived that their oral health influenced their food preferences and attitudes towards food, their social interactions and their self-esteem.

Background

Poor oral health can have biological, behavioural and social impacts on quality of life among older adults (aged 65+ years). In terms of biological impacts, oral health impairments may cause older adults to avoid many types of foods. This shift in dietary pattern can lead to malnutrition among older people, undermine general health and negatively impact quality of life (QOL).

Materials …

"Having It Both Ways: Containing The Champions Of Feminism In Female-Led Origin And Solo Superhero Films", Jessica Taylor, Laura Glitsos

Research outputs 2014 to 2021

In this article, we consider the emerging trend of solo, female-led superhero films, and their repeated location in aesthetically distinct pasts or “closed moments.” This pastness, we contend, serves to distinguish the concerns of the protagonists, which are often read as feminist, as redundant for the contemporary audience. This framing is in keeping with a postfeminist cultural context, wherein feminist values and successes are celebrated, while simultaneously declared irrelevant.

We examine the historical or closed settings in Wonder Woman (2017), Wonder Woman 1984 (2020), Captain Marvel (2019) and Black Widow (2021), and consider how this collective investment in the past …

Mining For Sleep Data: An Investigation Into The Sleep Of Fly-In Fly-Out Shift Workers In The Mining Industry And Potential Solutions, Gemma Maisey

Theses: Doctorates and Masters

Shift work in the mining industry is a risk factor for sleep loss leading to impaired alertness, which may adversely impact health and safety risks. This risk is being increasingly recognised by leaders and shift workers in the mining industry, however, there is limited knowledge available on the extent of sleep loss and other potential contributing factors. Furthermore, knowledge of the efficacy of individual interventions to assist shift workers to improve their sleep, and the management of risk at an organisational level is scarce. This PhD thesis involved three studies. The first two studies involved the recruitment of 88 shift …

Comparative Study On Development Of Novel Catalytic Oxidation For Removing Emerging Contaminants, Abdul H. Asif

Theses: Doctorates and Masters

‘Wastewater treatment’ is not itself a new concept, where global water bodies have been polluted since the evolution of mankind. As a result of human activities including domestic, industrial and commercial practices, levels of water pollution through aqueous contaminants are increasing every day. Moreover, rapid industrialisation and recent human progress has given rise to a new classification of pollution, that of emerging contaminants (ECs). Further, these chemicals are highly resistant to biodegradation and are capable of travelling, where they can penetrate from one part of the world to another. Whilst traditional wastewater treatment techniques, such as adsorption, might be effective …

Towards Establishing A Fit-For-Purpose Regulatory Framework For Radiation Protection In Western Australia's Mining Industry: Evaluating Mine Worker Exposures To Naturally Occurring Radionuclides, Martin I. Ralph

Theses: Doctorates and Masters

Mining in the state of Western Australia (WA) formally commenced in the 1840s, and over the ensuing 180 years has evolved to be the epicentre of the Australian mining industry and a significant contributor to the national economy. The lithology of WA is replete with mineralisation that hosts uranium and “critical minerals” required for the global renewable energy sector. The state’s first uranium mine is under development, and high levels of activity are occurring in the state’s nascent critical minerals sector, with 168 WA-based companies pursuing rare earths-bearing minerals, 51 of which are actively drilling on their tenements.

WA’s mineral …

Experiments And Modelling Into Air Staging In A Fixed Bed Biomass Combustor, Awais Junejo

Theses: Doctorates and Masters

Combustion of biomass to produce heat and electricity has increased recently due to environmental concerns over the use of fossil fuels. Being CO2 neutral, biomass has the potential to replace solid fossil fuels, such as coal, in thermal power plants. Direct combustion of biomass is the most widely used thermochemical process to generate heat and electricity.

In staged biomass combustion, primary air is supplied beneath the fuel bed, with secondary air then provided above in the freeboard region. The freeboard can be divided into primary freeboard length (LI), which is upstream of secondary air, and a secondary freeboard length (LII), …