A Comparison Of Metabolite Extraction And Data Manipulation Methods For ¹H Nmr Spectroscopy Of Skeletal Muscle Using Chicken Breast Tissue, Hannah Underhill

Theses

¹H NMR has become an incredibly useful tool in the field of metabolomics. Many tissue types may be examined via ¹H NMR in order to obtain a quantitative metabolic profile for an infinite number of phenotypes. In this study, I have tested three methods of metabolite extraction in conjunction with three methods of data analysis, in an attempt to determine which combination of the six methods (if any) are appropriate for extracting aqueous metabolites from skeletal muscle tissue for metabolite description and quantification via ¹H NMR. Metabolite extraction methods tested the use of methanol/ chloroform/ water, methanol/ …

The Need For International Ai Activities Monitoring, Parviz Partow-Navid, Ludwig Slusky

Journal of International Technology and Information Management

This paper focuses primarily on the need to monitor the risks arising from the dual-use of Artificial Intelligence (AI). Dual-use AI technology capability makes it applicable for defense systems and consequently may pose significant security risks, both intentional and unintentional, with the national and international scope of effects. While domestic use of AI remains the prerogative of individual countries, the unregulated and nonmonitored use of AI with international implications presents a specific concern. An international organization tasked with monitoring potential threats of AI activities could help defuse AI-associated risks and promote global cooperation in developing and deploying AI technology. The …

Winter 2023, Southwestern Oklahoma State University Office Of Institutional Advancement And Alumni

Echoes from the Hill

From the President

Dear Southwestern Oklahoma State University (SWOSU) alumni, emeriti, & friends –

I hope that this issue of Echoes From The Hill finds you and yours well. Here on the SWOSU Hilltop we are reflecting on a year of enrollment growth and significant accomplishments. We are looking forward to reaching new heights in the year ahead.

In these pages you will be able to learn about many great things happening here at SWOSU! This past summer we broke ground on the Jerry & Margaret Hodge Center for Pharmacy & Rural Health. This was the first groundbreaking for an …

Environmental Factors Influencing Selection Of Cave Entrances As Nesting Sites By The Eastern Phoebe (Sayornis Phoebe), J. Foster Sims

Theses

The Eastern Phoebe (Saynoris phoebe) is a medium-sized flycatcher found throughout eastern North America that traditionally nested in cave entrance zones. However, with the onset of human civilization and the adoption of man-made structures (e.g., barns, porches, bridges), the Eastern Phoebe’s population has dramatically increased (over 90%) in recent years. There has been little recent research regarding the continued use of cave entrances as nesting sites by the Eastern Phoebe. In this study, I surveyed 50 cave entrances in northern Alabama and south-central Tennessee to determine the environmental factors that influence the use of cave entrances as nesting …

Testing Cold Atmospheric Pressure Plasma : A Model For Cleaning Single-Use Urinary Catheters To Prevent Infections, Elanie Francease Briggs

Theses

Urinary tract infections (UTIs) are one of the most abundant bacterial infections world-wide with over 10 million cases and 13,000 deaths each year in the United States alone. The use of urinary catheters in hospitals and in outpatients significantly increases the risk of catheter-associated urinary tract infections (CAUTI) that are promoted by uropathogenic biofilm formation. Uropathogenic Escherichia coli (UPEC) is the dominant cause for both UTI and CAUTI, which are becoming increasingly multidrug-resistant. Uropathogenic biofilm accumulation on urinary catheters is very resistant to treatment by traditional sterilization methods. Cold atmospheric pressure plasmas (CAPP) have been known for their strong microbicidal …

N Y State Dent J January 2023

The New York State Dental Journal

In the January 2023 issue, the reader will find the following feature articles:

• Evaluating Repeat Dental Emergency Visits Related to Third-Molar Pathologies in an Urgent Dental Care Center
• Cultural Gingival Tattoo Diagnosis and Origins
• Diagnosis and Management of Nasopalatine Duct Cyst

This issue includes regular columns with regional news impacting the New York membership including: editorial and perspectives columns, legal, association activities, component news, continuing education opportunities, and classifieds.

Kennedy V. Google, Northern District Of California

Historical and Topical Legal Documents

No abstract provided.

“My Ob/Gyn Has The Worst Bedside Manner…” A Qualitative Analysis Of Patient-Provider Communication And Mental Health Outcomes Of Women In Rural Georgia, Tobi F. Oloyede

College of Graduate Studies: Theses & Dissertations

Despite technological advances in medicine and public health innovations, maternal mortality in the United States remains significantly higher than in other developed countries. Specifically, Georgia ranks as the second worst place to give birth in the country. Maternal mental health conditions are common, manageable complications that can happen at any point during pregnancy, childbirth, or in the first year following delivery, with 1 in 5 women affected. Unfortunately, 75% of women who experience MMH symptoms are left untreated.

This study focuses on patient-provider communication through the birthing experiences of women who have been pregnant in Georgia to examine how their …

Assessment Of Atmospheric Correction Algorithms For The Remote Sensing Of Water Quality In Southeastern U.S. Estuaries, Jerome Reimers

College of Graduate Studies: Theses & Dissertations

Water quality is a key indicator in understanding and representing an environment's overall health. Through developments in remote sensing, we can utilize satellite imagery to measure water parameters in each aquatic system. When accurate atmospheric correction is performed, remote sensing can account for atmospheric attenuation and scattering effects to better measure the reflectance and estimate optically active constituents (OAC) present in upper water columns. Atmospheric Correction for OLI lite (ACOLITE) is an atmospheric correction algorithm designed specifically for robust atmospheric correction of water surfaces, in comparison to algorithms designed more for land surfaces such as the European Space Agency’s (ESA) …

Gene Expression Under Combined Hypoxia And Acidosis In Chondrosarcoma, Michael Stacey, Kostika Vangjeli, Christopher Osgood

Bioelectrics Publications

Chondrosarcomas are the second most common cause of bone cancer and are removed surgically with wide margins. On recurrence, they are resistant to chemo and radiation therapy and new treatment options are critically required. This tumor type produces hyaline cartilage, a cartilage normally formed under hypoxic and acidic environment due to lack of vasculature in cartilage. Paradoxically, chondrosarcomas arise in the well vascularized, oxygen rich environment of the bone. Hypoxia and acidosis are two stressors where the cellular effects are typically reported separately even though cells experience combined effects of hypoxia and acidosis. Given the mechanistic links between hypoxia and …

Extracellular Vesticles In Acute Respiratory Distress Syndrome: Understanding Protective And Harmful Signaling For The Development Of New Therapeutics, Matthew Bavuso, Noel Miller, Joshua M. Sill, Anca Dobrian, Ruben M. L. Colunga Biancatelli

Bioelectrics Publications

Acute respiratory distress syndrome (ARDS) is a severe respiratory condition characterized by increased lung permeability, hyper-inflammatory state, and fluid leak into the alveolar spaces. ARDS is a heterogeneous disease, with multiple direct and indirect causes that result in a mortality of up to 40%. Due to the ongoing Covid-19 pandemic, its incidence has increased up to ten-fold. Extracellular vesicles (EVs) are small liposome-like particles that mediate intercellular communication and play a major role in ARDS pathophysiology. Indeed, they participate in endothelial barrier dysfunction and permeability, neutrophil, and macrophage activation, and also in the development of a hypercoagulable state. A more …

Interactions Of Carboxylated Nanodiamonds With Mouse Macrophages Cell Line And Primary Cells, Maisoun Bani-Hani, Stephen J. Beebe, Michael W. Stacey, Christopher Osgood

Bioelectrics Publications

Nanodiamonds (ND) have attracted significant interest for their use in several biomedical applications. These applications can be very useful if the safety and compatibility of ND are proven. We assessed the effects of ND (100 nm, Carboxylated) on primary macrophages and a macrophage-like cell line and found that these particles are not toxic to these cells at lower concentrations but may interfere with cell functions and differentiation. Internalization of ND by these cells in a time- and dose-dependent manner was mostly via phagocytosis and clathrin-dependent endocytosis and localized to the cytoplasm but not into the nucleus. No significant induction of …

Pulsed Electric Field Ablation Of Esophageal Malignancies And Mitigating Damage To Smooth Muscle: An In Vitro Study, Emily Gudvangen, Uma Mangalanathan, Iurii Semenov, Allen S. Kiester, Mark A. Keppler, Bennett L. Ibey, Joel N. Bixler, Andrei G. Pakhomov

Bioelectrics Publications

Cancer ablation therapies aim to be efficient while minimizing damage to healthy tissues. Nanosecond pulsed electric field (nsPEF) is a promising ablation modality because of its selectivity against certain cell types and reduced neuromuscular effects. We compared cell killing efficiency by PEF (100 pulses, 200 ns–10 µs duration, 10 Hz) in a panel of human esophageal cells (normal and pre-malignant epithelial and smooth muscle). Normal epithelial cells were less sensitive than the pre-malignant ones to unipolar PEF (15–20% higher LD50, p < 0.05). Smooth muscle cells (SMC) oriented randomly in the electric field were more sensitive, with 30–40% lower LD50 (p < 0.01). Trains of ten, 300-ns pulses at 10 kV/cm caused twofold weaker electroporative uptake of YO-PRO-1 dye in normal epithelial cells than in either pre-malignant cells or in SMC oriented perpendicularly to the field. Aligning SMC with the field reduced the dye uptake fourfold, along with a twofold reduction in Ca²⁺ transients. A 300-ns pulse induced a twofold smaller transmembrane potential in cells aligned with the field, making them …

Insights Into The Conservation Of The Rare Perennial Herb, Astragalus Applegatei, Sean Stewart

Cal Poly Humboldt theses and projects

As anthropogenic activities expand, conservation of rare and endangered species is critical to reduce extinction risks. Collecting and analyzing demographic data can assist in the development of conservation strategies and can help evaluate the success of existing strategies. In this study, I built integral projection models from five years of demographic data and conducted a population viability analysis on four populations of the rare perennial herb, Astragalus applegatei, to inform conservation decisions. I also assessed how planting A. applegatei seedlings might contribute to the growth of an extant population. I found that one population was already below the extinction …

Is The Area Postrema Resistant To The Anesthetic Effects Of Ms-222?, Austin T. Csiszar

Cal Poly Humboldt theses and projects

MS-222 is a common fish anesthetic that dampens neural activity by inhibiting neuronal voltage-gated sodium channels (Na_v) required for action potentials. Previous experiments conducted by our lab’s primary investigator using fluorescence calcium imaging in zebrafish neurons resulted in the observation that neurons in the area postrema (AP), a circumventricular organ located in the dorsal hindbrain, displayed prominent fluorescence intensity during MS-222 induced sedation. I used the genetically encoded calcium indicator GCaMP6s to conduct fluorescence calcium imaging with the goal of recording neural activity in the larval zebrafish area postrema and optic tectum to compare the anesthetic effects of …

The Effects Of Physical Education On Social Outcomes In Children With Disabilities: A Meta-Analysis, Alyette J. Afont

Cal Poly Humboldt theses and projects

Social outcomes are the foundation of human relationships and many children with disabilities may display deficits in the necessary behaviors to form those connections. Previous literature shows that physical activity can be an effective intervention for children with disabilities to promote social outcomes through the three domains of learning – cognitive, psychomotor, and affective. The purpose of this meta-analysis is to collect, index, and evaluate the available literature exploring how a physical education program can impact social outcomes in children with disabilities. By composing articles pertaining to social outcomes and physical education through multiple databases, researchers will be able to …

Indigenous Women In Active Drug Abuse Recovery: An Analysis Of Native And Non-Native Programs, Raquel J. Muñoz

Cal Poly Humboldt theses and projects

In general, much has been written on the experiences of prototypical women in drug recovery programs, however there is only a scarcity of research on the experiences of rural women of color in drug recovery programs. Very few Northern American cultures had experience with alcohol before the first wave of European settlers. Responses to intergenerational trauma faced by Native women include substance abuse, depression, anxiety, low self-esteem, suicidal thinking, and more. Due to socioeconomic disadvantages drug and alcohol abuse tends to be a coping mechanism for many Native American women. Drawing on the narratives of ten Indigenous women who are …

Olfactory Organ Morphology And Ultrastructure Of The Larval Koh Tao Island Caecilian (Ichthyophis Kohtaoensis), Jaclyn M. Patmore

Cal Poly Humboldt theses and projects

The olfactory system of living amphibians (Lissamphibia) undergoes major changes as it transitions from an aquatic to a terrestrial system during metamorphosis. Patterns of change in the cellular morphology of the nose have been examined for frogs (Anura) and salamanders (Caudata). However, it remains unknown if caecilians (Gymnophiona) have similar patterns of change in their nasal ultrastructure. In particular, no data on larval caecilian olfactory cell types are available. Here, using light microscopy and transmission electron microscopy, I examined the olfactory organ of larvae of the caecilian Ichthyophis kohtaoensis, to establish the ultrastructure of the epithelium and compare it …

Techno-Economic Analysis Of A Utility-Scale Solar Power Plant In Bhutan., Minjur

Cal Poly Humboldt theses and projects

Solar Photovoltaic (PV) technology, which captures energy from the sun, is a rapidly growing technology. Bhutan’s heavy dependence on a single energy source, hydropower, has become a significant concern for energy security. To address this, the Government of Bhutan intends to diversify energy sources using alternative renewable sources.

This research determined that Bhutan has the total potential to develop 3 gigawatts (GW) of solar power based on the utilization of about 7,100 acres of suitable land. The study also identified Tenchhekha, near the capital city of Thimphu, as a potential solar power plant location, with a capacity of 4.85 MWp …

Negative Social Media And Its Influence On Athlete's Performance, Bernd R. Huber

Cal Poly Humboldt theses and projects

This study aimed to investigate the potential impact of negative social media content on athletes' cortisol levels and subsequent performance. The study focused on the change in cortisol levels and differences in free throw performance, based on previous research findings. We hypothesized that negative social media postings would increase the stress experienced by student-athletes, resulting in elevated cortisol levels and decreased performance. Additionally, participants (n = 8) completed a questionnaire to examine the interaction between preexisting fear and the biological stress response. Contrary to expectations, there was no significant change in stress response, and negative postings did not have …

Let’S Talk About Series, Matthew B. Christensen

BYU Studies

A couple of years ago, Deseret Book began publishing the Let’s Talk About series, books designed to help readers learn about interesting—and potentially difficult—topics. The books are relatively short, ranging from 130 to 168 pages. Each book measures 4¼ × 7 inches, making them the perfect size to fit into a pocket or purse. The tone of each book is conversational and engaging. The authors are as varied as the subjects, recognized authorities in their respective fields and believing Latter-day Saints. The series began in 2021, and nine volumes have been published as of summer 2023.

An Analysis Of The Inter-State Similarity Of The Herpetofaunas Of Mexican States, Julio A. Lemos-Espinal, Geoffrey R. Smith

Faculty Publications

No abstract provided.

Variable Selection And Estimation In Bayesian Causal Mediation Analysis, Shuang Wang

Open Access Master's Theses

Causal mediation analysis has emerged as a valuable tool in various fields, allowing for decomposing total effects into direct and indirect effects. However, identifying the mediation effects relies on assumptions of no unmeasured confounding in the exposure-mediator, exposure-outcome, and mediator-outcome relationships. Moreover, there is often an interest in examining the effects of a mixture of exposures on the outcome, which poses challenges in determining the appropriate set of confounding variables. Therefore, we proposed a Bayesian mediation analysis that simultaneously selects common confounding variables associated with the two exposures, mediator, and outcome, by employing spike and slab group lasso using MCMC …

Plastic Recognition And Electrogenic Uniport Translocation Of 1st-, 2nd-, And 3rd-Row Transition And Post-Transition Metals By Primary-Active Transmembrane P1b-2-Type Atpase Pumps, Sameera S. Abeyrathna, Nisansala S. Abeyrathna, Priyanka Basak, Gordon W. Irvine, Limei Zhang, Gabriele Meloni

Department of Biochemistry: Faculty Publications

Transmembrane P-type ATPase pumps catalyze the extrusion of transition metal ions across cellular lipid membranes to maintain essential cellular metal homeostasis and detoxify toxic metals. Zn()-pumps of the P-type subclass, in addition to Zn , select diverse metals (Pb, Cdand Hg) at their transmembrane binding site and feature promiscuous metal-dependent ATP hydrolysis in the presence of these metals. Yet, a comprehensive understanding of the transport of these metals, their relative translocation rates, and transport mechanism remain elusive. We developed a platform for the characterization of primary-active Zn()-pumps in proteoliposomes to study metal selectivity, translocation events and transport mechanism in real-time, …

Examining The Regulation Of The Bs21 Homologs In Francisella Tularensis, Sierra Schmidt

Open Access Master's Theses

Francisella tularensis is a facultative intracellular pathogen, which relies on a network of regulation for its virulence. It contains three homologs of the ribosomal protein bS21. These three ribosomal protein homologs can independently associate with ribosomes, leading to ribosomal heterogeneity. The second homolog, bS21-2, is implicated in the positive regulation of a number of virulence proteins. Additionally, transcriptomic data indicates that bS21-2 is autoregulated. The mechanism by which this autoregulation occurs is unknown, as is the regulation of the other two homologs, bS21-1 and bS21-3. In the following chapters, I will show that bS21-1 and bS21-3 do not autoregulate their …

Poverty And Critical Theory, David Ingram

Philosophy: Faculty Publications and Other Works

This chapter surveys the various critical theory approaches from Marx to the present in the study of poverty and underdevelopment in relationship to capitalism, democracy, and intersectionality.

Social Care For Caregivers: How Washington Can Guarantee Unemployment Insurance For Working Caregivers, Victoria Kroeger

Seattle Journal for Social Justice

No abstract provided.

Decision-Making Processes, Rachel Cohen

Seattle Journal for Social Justice

No abstract provided.

The Role Of Myocardin In The Progression Of Non-Small Cell Lung Cancer, Soromidayo Akinsiku

Biotechnology Theses

Lung cancer is the leading cause of cancer-related mortality in the world and NSCLC accounts for 85% of all lung cancer cases. The mainstay of treatment for patients with stage I, II and IIIA NSCLC is surgery, followed by post-operative cisplatin-based chemotherapy. Additional adjuvant therapy involving targeted tyrosine kinase inhibitors has been in use, however even for the targeted therapy, resistance eventually develops. Therefore, there is a need for identifying novel targets for this life-threatening disease. Given that preliminary studies in Ikebe lab revealed that myocardin knockdown significantly promoted caspase-3 degradation, in this study, using myocardin siRNA, we investigated the …

Budget Oversight Policy 4th Quarter Budget Review Fy 2023-24, Csusb Budget Office

University Budget Office records

No abstract provided.