Digital Commons Network^™

Randomized Phase Ii Trial Of Dendritic Cell/Myeloma Fusion Vaccine With Lenalidomide Maintenance After Upfront Autologous Hematopoietic Cell Transplantation For Multiple Myeloma: Bmt Ctn 1401., David J Chung, Nina Shah, Juan Wu, Brent Logan, Lina Bisharat, Natalie Callander, Giulia Cheloni, Kenneth Anderson, Thinle Chodon, Binod Dhakal, Steve Devine, Poorvi Somaiya Dutt, Yvonne Efebera, Nancy Geller, Haider Ghiasuddin, Peiman Hematti, Leona Holmberg, Alan Howard, Bryon Johnson, Dimitra Karagkouni, Hillard M Lazarus, Ehsan Malek, Philip Mccarthy, David Mckenna, Adam Mendizabal, Ajay Nooka, Nikhil Munshi, Lynn O'Donnell, Aaron P Rapoport, Jane Reese, Jacalyn Rosenblatt, Robert Soiffer, Dina Stroopinsky, Lynne Uhl, Ioannis S Vlachos, Edmund K Waller, James W Young, Marcelo C Pasquini, David Avigan

Oncology Articles

PURPOSE: Vaccination with dendritic cell (DC)/multiple myeloma (MM) fusions has been shown to induce the expansion of circulating multiple myeloma-reactive lymphocytes and consolidation of clinical response following autologous hematopoietic cell transplant (auto-HCT).

PATIENTS AND METHODS: In this randomized phase II trial (NCT02728102), we assessed the effect of DC/MM fusion vaccination, GM-CSF, and lenalidomide maintenance as compared with control arms of GM-CSF and lenalidomide or lenalidomide maintenance alone on clinical response rates and induction of multiple myeloma-specific immunity at 1-year posttransplant.

RESULTS: The study enrolled 203 patients, with 140 randomized posttransplantation. Vaccine production was successful in 63 of 68 patients. At …

The Student Movement Volume 108 Issue 10: Vp Or Not Vp?: That Is The Question, Grace No, Lauren Kim, Nick Bishop, Nate Miller, Amelia Stefanescu, Katie Davis, Lily Burke, Kiheon Chung, Robert Zhang, Andrew Francis, Erin Beers, Reagan Westerman, Bella Hamann, Anna Rybachek, Sumin Lee, Alyssa Caruthers

The Student Movement v. 108 (2023-2024)

HUMANS

Interview with Dr. Ponce-Rodas: Change within the Church, Grace No

Dean Spotlight Interview: Alyssa Palmer, Lauren Kim

Maya Sukumaran's Exploration: Unraveling the Neurobiology of Cricket Behavior, Nick Bishop

ARTS & ENTERTAINMENT

AUSO's Vienna Classics Concert, Nate Miller

Discovering the Fine Arts Program, Amelia Stefanescu

What Do Murder and Law School Have in Common?, Amelia Stefanescu

NEWS

AU Abroad, Katie Davis

Context, Changes, Reactions, What's Coming, VP to the Assistant to the President, Lily Burke

Founding of Andrews University, Kiheon Chung

Israel-Gaza Follow Up, Robert Zhang

Second Annual AU Shark Tank Features Intriguing Proposals, Andrew Francis

IDEAS

Coming Out Ministries …

Neutralizing Antibodies Against Ebv Gp42 Show Potent In Vivo Protection And Define Novel Epitopes, Qian Wu, Ling Zhong, Dongmei Wei, Wanlin Zhang, Junping Hong, Yinfeng Kang, Kaiyun Chen, Yang Huang, Qingbing Zheng, Miao Xu, Mu-Sheng Zeng, Yi-Xin Zeng, Ningshao Xia, Qinjian Zhao, Claude Krummenacher, Yixin Chen, Xiao Zhang

College of Science & Mathematics Departmental Research

Epstein-Barr virus (EBV) is the first reported human oncogenic virus and infects more than 95% of the human population worldwide. EBV latent infection in B lymphocytes is essential for viral persistence. Glycoprotein gp42 is an indispensable member of the triggering complex for EBV entry into B cells. The C-type lectin domain (CTLD) of gp42 plays a key role in receptor binding and is the major target of neutralizing antibodies. Here, we isolated two rabbit antibodies, 1A7 and 6G7, targeting gp42 CTLD with potent neutralizing activity against B cell infection. Antibody 6G7 efficiently protects humanized mice from lethal EBV challenge and …

A Low-Power Analog Cell For Implementing Spiking Neural Networks In 65 Nm Cmos, John S. Venker, Luke Vincent, Jeff Dix

Computer Science and Computer Engineering Faculty Publications and Presentations

A Spiking Neural Network (SNN) is realized within a 65 nm CMOS process to demonstrate the feasibility of its constituent cells. Analog hardware neural networks have shown improved energy efficiency in edge computing for real-time-inference applications, such as speech recognition. The proposed network uses a leaky integrate and fire neuron scheme for computation, interleaved with a Spike Timing Dependent Plasticity (STDP) circuit for implementing synaptic-like weights. The low-power, asynchronous analog neurons and synapses are tailored for the VLSI environment needed to effectively make use of hardware SSN systems. To demonstrate functionality, a feedforward Spiking Neural Network composed of two layers, …

2022/2023 Robert E. Kennedy Library Annual Report, Robert E. Kennedy Library

Library Publications and Administrative Reports

This year’s Annual Report is the first “outside” edition of Inside 35 following the closure of the library’s home, Building 35, in June 2023. Kennedy Library remains an active partner in Cal Poly’s curricular and scholarly endeavors from many locations across campus. Learn about our Librarian-Scholars collaborating with students and colleagues on research projects across several disciplines. Discover the comprehensive collection of the works of Joe D’Ambrosio that was generously donated to Special Collections and Archives in 2019. Read an update on the building transformation project and meet the Design-Build Team of Hensel-Phelps and the Miller Hull Partnership. Kennedy Library …

Composition And Function Of The Galapagos Penguin Gut Microbiome Vary With Age, Location, And A Putative Bacterial Pathogen, Sage D. Rohrer, Gustavo Jiménez-Uzcátegui, Patricia G. Parker, Lon M. Chubiz

Biology Department Faculty Works

Microbial colonization plays a direct role in host health. Understanding the ecology of the resident microbial community for a given host species is thus an important step for detecting population vulnerabilities like disease. However, the idea of integrating microbiome research into conservation is still relatively new, and wild birds have received less attention in this field than mammals or domesticated animals. Here we examine the composition and function of the gut microbiome of the endangered Galapagos penguin (Spheniscus mendiculus) with the goals of characterizing the normal microbial community and resistome, identifying likely pathogens, and testing hypotheses of structuring forces for …

Families With Fathers In Minimum Security Prison: A Family Treatment Approach To Treating Families With Fathers In Prison, Alexandria Davis, Madison Schrack

Student Projects

The population of those in prison is increasing, and many of those who are in prison have families. Much research has been done on the effect prison has on prisoners, but not as much has looked at the children and what the effect is of having a parent, specifically their father, who is absent in prison. It is important to understand the possible behaviors, risks, and challenges that children face. This research essay looks at what it is like for children between the ages of 5 and 10 to have a father in minimum security prison while also examining the …

The Alliance Of Genome Resources: Transforming Comparative Genomics., Carol J Bult, Paul W Sternberg

Faculty Research 2023

Comparing genomic and biological characteristics across multiple species is essential to using model systems to investigate the molecular and cellular mechanisms underlying human biology and disease and to translate mechanistic insights from studies in model organisms for clinical applications. Building a scalable knowledge commons platform that supports cross-species comparison of rich, expertly curated knowledge regarding gene function, phenotype, and disease associations available for model organisms and humans is the primary mission of the Alliance of Genome Resources (the Alliance). The Alliance is a consortium of seven model organism knowledgebases (mouse, rat, yeast, nematode, zebrafish, frog, fruit fly) and the Gene …

The Status And Prospects Of Phytoremediation Of Heavy Metals, Aniruddha Acharya, Enrique Perez, Miller Maddox-Mandolini, Hania De La Fuente

Faculty Publications - Biological Sciences

The release of heavy metals into the agricultural soil and waterbodies has been accelerated due to anthropogenic activities. They are not usually required for biological functions thus, their accumulation in biological system poses serious threat to health and environment globally. Phytoremediation offers a safe, inexpensive, and ecologically sustainable technique to clean habitats contaminated with heavy metals. Though several plants have been identified and used as a potential candidate for such phytoremediation, the technique is still at its formative stage and has been mostly confined to laboratory and greenhouses. However, recently several field studies have shown promising results that can propel …

Unpacking The Unconscious: Mortality Awareness And Its Effects On Consumer Engagement In Charitable Campaigns, Lois Warden

Honors Projects in Marketing

This research aims to investigate the interplay between terror management theory, self-other message framing, and psychological distance in influencing individuals’ perceptions, judgements, and behavioral responses, particularly in context of advertisements featuring animals of varying proximity to humans. The purpose of this research is to understand how mortality sensitivity and the categorizations of ‘self’ and ‘other’ impact individuals’ attitudes and intentions towards advertisements featuring animals. The research methodology employed involves experimental designs with manipulated salience of priming conditions (birth or death) and the presentation of animals (monkey as proxy to self and frog as distal to self) to gauge participants attitudes, …

Winter Commencement, 2023, Marshall University

Marshall University Commencement

Program for the One Hundred Eighty-Eighth Commencement of Marshall University, Saturday, December 9 2023 held at the Mountain Health Arena in Huntington, West Virginia.

Mycorrhizal Feedbacks Influence Global Forest Structure And Diversity, Camille S. Delavaux, Joseph A. Lamanna, Jonathan A. Myers, Richard P. Phillips, Salomón Aguilar, David Allen, Alfonso Alonso, Kristina J. Anderson-Teixeira, Matthew E. Baker, Jennifer L. Baltzer, Pulchérie Bissiengou, Mariana Bonfim, Norman A. Bourg, Warren Y. Brockelman, David F.R.P. Burslem, Li Wan Chang, Yang Chen, Jyh Min Chiang, Chengjin Chu, Keith Clay, Susan Cordell, Mary Cortese, Jan Den Ouden, Christopher Dick, Sisira Ediriweera, Erle C. Ellis, Anna Feistner, Amy L. Freestone, Thomas Giambelluca, Christian P. Giardina, Gregory S. Gilbert, Fangliang He, Jan Holík, Robert W. Howe, Walter Huaraca Huasca, Stephen P. Hubbell, Faith Inman, Patrick A. Jansen, Daniel J. Johnson, Kamil Kral, Sabrina E. Russo, Et Al.

Center for Plant Science Innovation: Faculty Publications

One mechanism proposed to explain high species diversity in tropical systems is strong negative conspecific density dependence (CDD), which reduces recruitment of juveniles in proximity to conspecific adult plants. Although evidence shows that plant-specific soil pathogens can drive negative CDD, trees also form key mutualisms with mycorrhizal fungi, which may counteract these effects. Across 43 large-scale forest plots worldwide, we tested whether ectomycorrhizal tree species exhibit weaker negative CDD than arbuscular mycorrhizal tree species. We further tested for conmycorrhizal density dependence (CMDD) to test for benefit from shared mutualists. We found that the strength of CDD varies systematically with mycorrhizal …

Beyond N-Dopants For Organic Semiconductors: Use Of Bibenzo[D]Imidazoles In Uv-Promoted Dehalogenation Reactions Of Organic Halides, Kan Tang, Megan R. Brown, Chad Risko, Melissa K. Gish, Garry Rumbles, Phuc H. Pham, Oana R. Luca, Stephen Barlow, Seth R. Marder

UK CARES Faculty Publications

2,2’-Bis(4-dimethylaminophenyl)- and 2,2'-dicyclohexyl-1,1',3,3'-tetramethyl-2,2',3,3'-tetrahydro-2,2'-bibenzo[d]imidazole ((N-DMBI)2 and (Cyc-DMBI)2) are quite strong reductants with effective potentials of ca. −2 V vs ferrocenium/ferrocene, yet are relatively stable to air due to the coupling of redox and bond-breaking processes. Here, we examine their use in accomplishing electron transfer-induced bond-cleavage reactions, specifically dehalogenations. The dimers reduce halides that have reduction potentials less cathodic than ca. −2 V vs ferrocenium/ferrocene, especially under UV photoexcitation (using a 365 nm LED). In the case of benzyl halides, the products are bibenzyl derivatives, whereas aryl halides are reduced to the corresponding arenes. The potentials of the halides that can be …

Strategic Approach To The Design Of Polymers From Natural Oils, Zoran Petrović, Jian Hong

National Institute for Materials Advancement

Natural oils are a large group of substances with varied compositions and properties. Thus, their utilization in polymers must be carefully planned. Generally, oils are less pure starting materials for the synthesis of polymers compared to petrochemicals, which may have a negative or positive influence on their applications. Designing oils with a desired structure by genetic modifications for a given application is an exciting future development. This chapter [1] tries to systematically analyze the influence of structural factors on the properties and the technologies used to obtain desired products.

Association Between Microbiome And The Development Of Adverse Posttraumatic Neuropsychiatric Sequelae After Traumatic Stress Exposure, Abigail L. Zeamer, Marie Claire Salive, Xinming An, Francesca L. Beaudoin, Stacey L. House, Jennifer S. Stevens, Donglin Zeng, Thomas C. Neylan, Gari D. Clifford, Sarah D. Linnstaedt, Scott L. Rauch, Alan B. Storrow, Christopher Lewandowski, Paul I. Musey, Phyllis L. Hendry, Sophia Sheikh, Christopher W. Jones, Brittany E. Punches, Robert A. Swor, Lauren A. Hudak, Jose L. Pascual, Mark J. Seamon, Erica Harris, Claire Pearson, David A. Peak, Roland C. Merchant, Robert M. Domeier, Niels K. Rathlev, Brian J. O’Neil, Paulina Sergot, Leon D. Sanchez, Steven E. Bruce

Psychology Faculty Works

Patients exposed to trauma often experience high rates of adverse post-traumatic neuropsychiatric sequelae (APNS). The biological mechanisms promoting APNS are currently unknown, but the microbiota-gut-brain axis offers an avenue to understanding mechanisms as well as possibilities for intervention. Microbiome composition after trauma exposure has been poorly examined regarding neuropsychiatric outcomes. We aimed to determine whether the gut microbiomes of trauma-exposed emergency department patients who develop APNS have dysfunctional gut microbiome profiles and discover potential associated mechanisms. We performed metagenomic analysis on stool samples (n = 51) from a subset of adults enrolled in the Advancing Understanding of RecOvery afteR traumA …

Use Of Serial Smartphone-Based Assessments To Characterize Diverse Neuropsychiatric Symptom Trajectories In A Large Trauma Survivor Cohort, Francesca L. Beaudoin, Xinming An, Archana Basu, Yinyao Ji, Mochuan Liu, Ronald C. Kessler, Robert F. Doughtery, Donglin Zeng, Kenneth A. Bollen, Stacey L. House, Jennifer S. Stevens, Thomas C. Neylan, Gari D. Clifford, Tanja Jovanovic, Sarah D. Linnstaedt, Laura T. Germine, Scott L. Rauch, John P. Haran, Alan B. Storrow, Christopher Lewandowski, Paul I. Musey, Phyllis L. Hendry, Sophia Sheikh, Christopher W. Jones, Brittany E. Punches, Michael C. Kurz, Robert A. Swor, Vishnu P. Murty, Meghan E. Mcgrath, Lauren A. Hudak, Jose L. Pascual, Elizabeth M. Datner

Psychology Faculty Works

The authors sought to characterize adverse posttraumatic neuropsychiatric sequelae (APNS) symptom trajectories across ten symptom domains (pain, depression, sleep, nightmares, avoidance, re-experiencing, anxiety, hyperarousal, somatic, and mental/fatigue symptoms) in a large, diverse, understudied sample of motor vehicle collision (MVC) survivors. More than two thousand MVC survivors were enrolled in the emergency department (ED) and completed a rotating battery of brief smartphone-based surveys over a 2-month period. Measurement models developed from survey item responses were used in latent growth curve/mixture modeling to characterize homogeneous symptom trajectories. Associations between individual trajectories and pre-trauma and peritraumatic characteristics and traditional outcomes were compared, along …

Evaluation Of Current Monitoring Practices For Patients Receiving Infliximab, Yujin Hong Pharmd, Michael Schaedler Pharmd, Bcps, Bcop, Vincent Do Pharmd, Bcps, Bctxp

Posters

• Infliximab is used for a wide range of inflammatory conditions including Crohn’s disease, ulcerative colitis, rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis, and plaque psoriasis.
• Patients should be closely monitored for development of signs and symptoms of active tuberculosis and latent infection, hepatitis B virus, and human immunodeficiency virus (HIV) during treatment with infliximab following termination of therapy.
• Hematologic abnormalities have also been reported in patients receiving infliximab and caution should be exercised in patients being treated with infliximab who have ongoing or a history of significant hematologic abnormalities.
• Patients with symptoms of signs of liver dysfunction should be evaluated for …

Kejahatan Transnasional Di Indonesia: Studi Kasus Yakuza Sebagai Organisasi Kejahatan Internasional, Adi Saputra Rusli, Yanwar Iswahyudi Mansur, Nisrina Arumdanie, Biky Uthbek Mubarok

Jurnal Kajian Stratejik Ketahanan Nasional

This article presents an analysis of the Yakuza phenomenon, an organized crime organization with deep historical roots in Japan. Through a qualitative approach, this research explores the history and development of the Yakuza, their criminal focus, operational networks, and their impact both national and international levels. The Japanese government's efforts to combat the Yakuza, on a national and global scale, are detailed, including programs for drug prevention, anti-human trafficking, and counter-terrorism. In an international context, the Yakuza is known for its extensive networks, creating new challenges in global security. This research also highlights the importance of global efforts to counter …

Liquid Chromatography Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Elisha Ogbebor

Masters Theses & Specialist Projects

Bovine mastitis is caused by a wide range of pathogens which results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the innate immune system and act as the first line of defense against mastitis in bovine. β-defensins (~6- 10kDa) are antimicrobial peptides that contain about 28 to 67 amino acids, the presence of six cysteine residues results in their three disulfide bonds and a β-sheet structure. A few β- defensins have been identified in cows based on the site of occurrence and time of expression in tissue, but not in milk. A …

Towards The Quantification Of Estrone And 17Β-Estradiol Conjugates In Dairy Cattle Urine Sorptive Stir Bar Extraction And Gas Chromatography-Mass Spectometry, S.M. Istiak Ahmed

Masters Theses & Specialist Projects

The global concern regarding the presence of compounds with estrogenic properties in the environment has grown significantly. Endocrine-disrupting compounds (EDCs) are environmental pollutants that disrupt the endocrine systems of both wildlife and humans. Estrogens, with their potent estrogenic effects, are particularly noteworthy among the various substances with endocrine-disrupting properties. They are of concern due to their prevalence in the environment, especially due to dairy waste contamination. These lipophilic compounds can accumulate in livestock fat tissues, potentially entering the human food chain. Sulfated forms of estrone and β- estriol, which exhibit a higher degree of saturation than their non-conjugated counterparts, have …

Bioactivity And Structural Properties Of Hydroxyapatite On Ti6a|4v And Si(100) Surfaces By Pulsed Laser Deposition, Salizhan Kylchbekov

Masters Theses & Specialist Projects

Although biomedical implant technology is very advanced, there are still caveats in terms of biocompatibility properties because metals are inert to biological processes such as osseointegration, cell growth, and cell adhesion. This results in statistically significant complications and opens rooms for improvements. Among many techniques to improve this problem, coating the metal surface with biologically functional materials has resulted in the best performances. Hydroxyapatite (HAP), Ca_₁₀(PO_₄)_₆(OH)_₂, is the most stable form of calcium phosphates in human body environment and is functional in key processes such as ion exchange, osteoblast (bone cells) formation, …

A Nonparametric Test For Comparing Survival Functions Based On Restricted Distance Correlation, Quinyang Zhang

Mathematical Sciences Faculty Publications and Presentations

In this article, we propose an omnibus test for comparing two survival functions under non-proportional hazards. The test statistic is based on a product-limit estimate of the restricted distance correlation, which is closely related to the L₂ distance between survival curves. The strong consistency is established under mild regularity conditions. Our simulation studies show that the new test has satisfactory power under proportional hazard and various non-proportional hazards settings including delayed treatment effect, diminishing effect, and crossing survival curves; therefore, it can be a competitive alternative to the existing omnibus tests such as Kolmogorov-Smirnov test, Cramer-von Mises test, two-stage …

Examining The Effects Of Hormonal Contraception On Injury In Collegiate Club And Recreation Sports, Kaitlyn Doherty

Honors College Theses

Background: There are differences among injury rates between males and females in collegiate sports. Hormonal contraception is used in females as a way of balancing hormones. There is limited research on the effects of hormonal contraception on injury outcomes in females.

Purpose: The purpose of this study was to examine the effects of hormonal contraceptives on injury in collegiate club and recreation sports.

Methods: A one-time survey was administered to all students enrolled in intramural sports at a mid-sized Southeastern Division I university. Questions were asked regarding injury history, menstrual cycle, and use of hormonal contraceptives.

Results: Analysis of survey …

Analyzing Functional Interactions Of Designed Peptides By Nmr Spectroscopy, Wonsuk Choi

Pharmaceutical Sciences (MS) Theses

The development of small peptide-based therapeutics can be accelerated by the knowledge of relationships between the peptide structure and its functional interactions. Here, we report the analysis of two groups of synthetic peptides designed for two applications – broad bactericidal action and inhibition of protein-protein interactions in human cells. Novel amphiphilic peptides designed for antibacterial application incorporated arginine as cationic amino acids and non-natural amino acids that have aromatic side chains with similar hydrophobic properties as tryptophan. The interaction of lead cyclic peptides and their linear analogs with a phospholipid bilayer mimicking a bacterial membrane was studied using nuclear magnetic …

Perfusability And Immunogenicity Of Implantable Pre-Vascularized Tissues Recapitulating Features Of Native Capillary Network, Dhavan Sharma, Archita Sharma, Linghao Hu, Te An Chen, Sarah Voon, Kayla J. Bayless, Jeremy Goldman, Alex J. Walsh, Feng Zhao

Michigan Tech Publications

Vascularization is a key pre-requisite to engineered anatomical scale three dimensional (3-D) constructs to ensure their nutrient and oxygen supply upon implantation. Presently, engineered pre-vascularized 3-D tissues are limited to only micro-scale hydrogels, which meet neither the anatomical scale needs nor the complexity of natural extracellular matrix (ECM) environments. Anatomical scale perfusable constructs are critically needed for translational applications. To overcome this challenge, we previously developed pre-vascularized ECM sheets with long and oriented dense microvascular networks. The present study further evaluated the patency, perfusability and innate immune response toward these pre-vascularized constructs. Macrophage-co-cultured pre-vascularized constructs were evaluated in vitro to …

+Protective Roles For Interferon-Gamma In Astrocytes During Chronic Autoimmunity, Brandon C. Smith

ETD Archive

Multiple sclerosis (MS) is an inflammatory and neurodegenerative disease of the central nervous system (CNS). Infiltrating inflammatory immune cells perpetuate demyelination and axonal damage in the CNS and significantly contribute to pathology and clinical deficits. Interferon (IFN)γ is classically described as deleterious in acute CNS autoimmunity, we and others have shown astrocytic IFNγ signaling also has a neuroprotective role. We found IFNγ signaling on astrocytes upregulates subunits of the immunoproteasome (iP) and programmed death ligand 1 (PD-L1), both of which serve protective roles during autoimmunity. Astrocyte-specific knockouts of Ifngr1 resulted in increased clinical severity in the MS murine model, experimental …

Exploration Of Secondary Structure Directing Agents On The Formation Of Hierarchical And 2d Zeolites, Suleiman Yahaya

ETD Archive

Hierarchical and 2D zeolites address the diffusion limitation of conventional zeolites by enhancing diffusion in the mesopores. So far, the surfactant compounds, which successfully produce hierarchical zeolites, have complex molecular structures making the zeolite synthesis expensive and uncompetitive for commercialization, making cost-effective synthesis of hierarchical and 2D zeolite highly desirable. However, the development of effective synthesis protocols relies on a good understanding of the SDA's influence on crystal morphology development, which is largely unknown. Here, we explore inexpensive and readily available compounds via a dual-SDA strategy and use MFI zeolite as the model system for the research. TPAOH served as …

Protective Responses Of The Intestinal Epithelial Cell Line Ht-29 Cells Exposed To Dephosphorylated Salmonella Flagellin [Research Note], Heyeon Baik, Jaiesoon Cho

The Philippine Agricultural Scientist

This study aimed to describe the effects of Salmonella Typhimurium flagellin (SFL) dephosphorylated by sweet potato purple acid phosphatase (PAP) on the protective responses of the intestinal epithelial cell line HT-29 cells. The enzyme was reported to display a broad substrate specificity for various organic phosphorylated conjugates and phosphoproteins. Dephosphorylation of SFL by sweet potato PAP decreased to 35% in the presence of 0.05 mM vanadate as compared with the negative control (p < 0.05). Intact SFL and the SFL treated with sweet potato PAP did not remarkably induce the activation of caspase-3 in HT-29 cells at all the tested levels of the substrate. Intact SFL maximally induced the release of IL (interleukin)-8 in HT-29 cells at 1000 ng/mL (p < 0.05). However, the SFL treated with the enzyme inhibited the release of IL-8 at over 100 ng/mL of the substrate as compared with intact SFL, resulting in an approximately 8-fold decrease even at 1000 ng/mL (p < 0.05). The SFL treated with the enzyme decreased the activation of the total ERK1/2 in the cells to 1.9 and 1.7–fold at 10 and 1000 ng/mL of the substrate, respectively, as compared with intact SFL (p < 0.05). In conclusion, sweet potato PAP could be a promising tool for controlling excessive inflammation during Salmonella infection in animal husbandry, and the enzyme could be a safe alternative that can overcome the drawbacks of chemotherapy.

An Objective Structured Clinical Examination For Anesthetic Management Of Pheochromocytoma, Shiar Rahaim

Doctoral Projects

Pheochromocytoma, whether recognized or unrecognized, has the potential to cause severe cardiovascular complications during surgery. This paper focuses on the creation and importance of an Objective Structured Clinical Examination (OSCE) and a short trigger film focused on the anesthetic management of pheochromocytoma. This OSCE intends to help prepare students to recognize and treat undiagnosed or improperly managed pheochromocytoma in the didactic phase of their learning so as to better prepare them for the possible real-life scenario.

The pathophysiology of pheochromocytoma regarding the catecholamines epinephrine and norepinephrine are discussed along with their potential to wreak havoc on the patient. The treatment …

The Perception Of Natural Resource Management In Nebraska: Efforts For Cross-Boundary Collaborative Management, Daniel Morales

School of Natural Resources: Dissertations, Theses, and Student Research

Nebraska’s agricultural landscapes are rapidly changing, affecting natural resources and their successful management. I utilized two surveys and scenario planning (Chapters 1: statewide survey, 2: local survey, and 3: scenario-planning workshop) to investigate attitudes and perceptions of natural resource management and cross-boundary collaboration. My first objective focused on determining what prevents Nebraskans from addressing natural resources challenges, considering demographics amongst generations and the type of areas they live in (rural versus urban). The second objective focused on whether landowners engaged with their community in managing natural resources. The third objective was to develop alternative future scenarios for the Denton Hills …