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Articles 24751 - 24780 of 35290
Full-Text Articles in Entire DC Network
Application Of A New Inoculant “Chikuso-1” For Silage Preparation Of Forage Paddy Rice, Y. Cai, C. Xu, S. Ennahar, N. Hino, N. Yoshida, M. Ogawa
Application Of A New Inoculant “Chikuso-1” For Silage Preparation Of Forage Paddy Rice, Y. Cai, C. Xu, S. Ennahar, N. Hino, N. Yoshida, M. Ogawa
IGC Proceedings (1977-2023)
Forage paddy rice is currently one of the most important silage crops in Japan. In fact, the use of paddy rice culture for silage production has been steadily increasing in recent years, not only because this represents a new way towards achieving self-sufficiency in animal feed, but also because of the interest of combining crop cultivation and livestock farming as a more effective use of idle paddy fields that often remain unused. However, the preparation of quality silage from paddy rice and its long-term storage are often challenging (Cai et al., 1999, 2003). In this study, a new bacterial …
The Influence Of The Application Of A Biological Additive On The Fermentation Process Of Red Clover Silage, Ľ. Rajčáková, R. Mlynár, M. Gallo
The Influence Of The Application Of A Biological Additive On The Fermentation Process Of Red Clover Silage, Ľ. Rajčáková, R. Mlynár, M. Gallo
IGC Proceedings (1977-2023)
In Slovakia, mainly in the submontane and mountainous regions, growing of red clover is an important source of proteinous feeds. It is grown on 3.0% of arable land. It was the aim of this work to verify the possibilities of using a biological additive in red clover silage conservation.
Synergism Of Chemical And Microbial Additives On Sugarcane (Saccharum Officinaruml.) Silage Fermentation, T. F. Bernardes, G. R. Siqueira, R. P. Schocken-Inturrino, A. P. T. P. Roth, R. A. Reis
Synergism Of Chemical And Microbial Additives On Sugarcane (Saccharum Officinaruml.) Silage Fermentation, T. F. Bernardes, G. R. Siqueira, R. P. Schocken-Inturrino, A. P. T. P. Roth, R. A. Reis
IGC Proceedings (1977-2023)
Sugarcane has a high productive potential (30 t DM/year) and it is commonly used in its fresh form. The ensiling of sugarcane is increasing but little research has been carried out to reduce nutrient losses during fermentation.
Effects Of Stage Of Growth And Inoculation On Fermentation Quality Of Field Pea Silage, G. Borreani, L. Cavallarin, S. Antoniazzi, E. Tobacco
Effects Of Stage Of Growth And Inoculation On Fermentation Quality Of Field Pea Silage, G. Borreani, L. Cavallarin, S. Antoniazzi, E. Tobacco
IGC Proceedings (1977-2023)
Field peas (Pisum sativum L.) are a short-term catch crop with a high crude protein content, which provides a high forage yield in a short growing period. Since field peas are a succulent crop and are difficult to field cure, it is preferable to directly ensile them to prevent weather damage and excessive grain losses. The onset of lodging is delayed in field pea varieties, since the crop is supported by the tendrils in a more erect manner, and this allows easy harvesting without soil contamination even at advanced stages of maturity (Koivisto et al., 2003). To …
Inoculant Effects On Ensiling And In Vitro Gas Production In Lucerne Silage, R. E. Muck, I. Filya, F. E. Contreras-Govea
Inoculant Effects On Ensiling And In Vitro Gas Production In Lucerne Silage, R. E. Muck, I. Filya, F. E. Contreras-Govea
IGC Proceedings (1977-2023)
Inoculants are the most common additives used in making silage. While inoculant effects on fermentation and dry matter (DM) recovery are understood, animal performance effects are often greater than expected. In vitro analyses may help uncover how inoculants affect rumen fermentation and ultimately dairy cattle performance. Our objective was to study how inoculation of lucerne silage affected in vitro gas production.
A Novel Bacterial Silage Additive Effective Against Clostridial Fermentation, E. Mayrhuber, M. Holzer, W. Kramer, E. Mathies
A Novel Bacterial Silage Additive Effective Against Clostridial Fermentation, E. Mayrhuber, M. Holzer, W. Kramer, E. Mathies
IGC Proceedings (1977-2023)
Silage quality is determined by factors including the content of butyric acid and ammonium-N. These parameters have to be restricted especially in lightly wilted silages due to a higher risk of clostridia contamination. In this study a novel silage additive was tested in grass silages of low dry matter content. The objective of this experiment was to explore the effect of the silage additive on quality parameters in comparison to an untreated control.
In Vitro Gas Production And Bacterial Biomass Estimation For Lucerne Silage Inoculated With One Of Three Lactic Acid Bacterial Inoculants, F. E. Contreras-Govea, R. E. Muck, I. Filya, D. R. Mertens, P. J. Weimer
In Vitro Gas Production And Bacterial Biomass Estimation For Lucerne Silage Inoculated With One Of Three Lactic Acid Bacterial Inoculants, F. E. Contreras-Govea, R. E. Muck, I. Filya, D. R. Mertens, P. J. Weimer
IGC Proceedings (1977-2023)
Silages inoculated with microbial inoculants frequently have a lower pH than non-inoculated crops. Less often inoculated crops have a positive effect on milk production (Weinberg & Muck, 1996). One hypothesis is that bacterial inoculants produce a probiotic effect that could enhance animal performance (Weinberg & Muck, 1996). Our objective was to use the method of Blümmel et al. (1997) to study differences in in vitro fermentation among lucerne silages inoculated with three microbial inoculants.
Paying For Our Keep: Grasslands Decision Support In More-Developed Countries, Andrew D. Moore
Paying For Our Keep: Grasslands Decision Support In More-Developed Countries, Andrew D. Moore
IGC Proceedings (1977-2023)
Key points
1. A survey of decision support (DS) tools in grassland agriculture illustrates the diversity of decisions supported and of delivery technologies that are used. Larger, whole-enterprise planning tools are undergoing a period where their user interfaces are being adapted to better reflect the requirements and practice of advisory users.
2. The history of use of GrassGro, a ‘versatile simulator’, is used to illustrate how versatile tools attract a diverse range of users and uses. Lessons learnt by the GrassGro team are discussed.
3. Uptake rates of DS tools in grasslands are generally lower than was expected a decade …
Survey Of Tetraploid And Diploid Perennial Pastures In The Waikato For Number Of Spores Produced By The Fungus Pithomyces Chartarum, J. P. J. Eerens, W. W. Nichol, J. Waller, J. M. Mellsop, M. R. Trolove, M. G. Norriss
Survey Of Tetraploid And Diploid Perennial Pastures In The Waikato For Number Of Spores Produced By The Fungus Pithomyces Chartarum, J. P. J. Eerens, W. W. Nichol, J. Waller, J. M. Mellsop, M. R. Trolove, M. G. Norriss
IGC Proceedings (1977-2023)
Facial eczema (FE) is a disease of livestock, caused by a toxin released into the bloodstream after digestion of spores of Pithomyces chartarum, a fungus residing in necrotic plant material in the base of pastures (di Menna & Bailey, 1973). Spore numbers tend to be highest in warm, humid conditions, where high post grazing residuals have lead to a build up of necrotic plant material. Tetraploid perennial ryegrass pastures tend to be more palatable, and with lower post gazing residuals, than equivalent diploid pastures; thus we hypothesised that spore numbers would be lower in tetraploid pastures. A survey of …
A Survey Of European Regional Adaptation In Italian Ryegrass Varieties, T. J. Gilliland, A. J. P. Wijk
A Survey Of European Regional Adaptation In Italian Ryegrass Varieties, T. J. Gilliland, A. J. P. Wijk
IGC Proceedings (1977-2023)
Ryegrass is widely adapted to cool temperate eco-zones and breeders often submit individual varieties for testing in a number of EU countries. National testing programmes often combine data from several trial sites that may differ climatically, but not from sites in other member states, despite the possibility of high ecological similarity. Given increasing interest in ‘animal value’ characters (soluble sugars, lipids, sward geometry), additional testing for these would be valuable but is prohibited by capped or declining funding. Data sharing between EU national authorities could be advantageous but is inhibited by the lack of statistically valid data on the sensitivity …
Correlation Between Epiphytic Microflora And Microbial Pollution And Fermentation Quality Of Silage Made From Grasses, B. Osmane, J. Blūzmanis
Correlation Between Epiphytic Microflora And Microbial Pollution And Fermentation Quality Of Silage Made From Grasses, B. Osmane, J. Blūzmanis
IGC Proceedings (1977-2023)
Grass forage in Latvia is the main and inexpensive cow feed, however its composition and nutritive value differ during the growth period of grasses. The traits of grasses, their natural ensilage capacity, count of epiphytic microflora, the timing of harvest and ensilage making technology affecting the quality of grass silage are important issues to be studied. Silage making for the winter period is the treatment of green material to minimise the breakdown of nutrients being the results of biochemical and microbiological processes. The aim of the research was to clarify the methodologies to reduce the count of epiphytic microflora and …
Survival In The City Streets: An Autoethnographic Analysis Challenging The Criminalization Of Urban Street Gangs, Robert Northman
Survival In The City Streets: An Autoethnographic Analysis Challenging The Criminalization Of Urban Street Gangs, Robert Northman
PSU McNair Scholars Online Journal
To varying outcomes, many attempts have been made to understand and define urban street gangs. Though these understandings and definitions of gangs may vary, they almost always rely on criminality as at least one of the gang’s “purposes.” The author, a gang member for approximately thirty years seeks to contribute lived experiences to the conversation centered on gangs. This auto-ethnographic analysis of urban street gang culture seeks to highlight the author’s experiences as potential determinants of gang membership while also exacting the realities of some common myths and other misconceptions surrounding the urban street gang, including the criminalization thereof. The …
Invasive Plant Species And Their Impact On Stream Health Using Macroinvertebrates In The Willamette Valley, Oregon, Michelle Hesek, Patrick Edwards
Invasive Plant Species And Their Impact On Stream Health Using Macroinvertebrates In The Willamette Valley, Oregon, Michelle Hesek, Patrick Edwards
PSU McNair Scholars Online Journal
Oregon spends approximately $83 million annually to maintain invasive plant species, which decreases resource availability for the environment and humanity. Their continued spread affects riparian zones, one of the most biologically diverse ecosystems that supplement stream and upland habitats. Some of the energy flow in these systems circulates by macroinvertebrates that decompose leaf litter during their immature stage of life. Upon metamorphosis, they rely on plants in riparian zones for reproductive habitat, making them prey to predators in stream and upland food webs, referred to as a transformational link. This research examines how invasive plant species affect stream health in …
The Effect Of Rhizophagus Intraradices Mycorrhizal Fungus On Iron Uptake Of Corn: A Global Overview On The Effects Of Climate Change On The Nutritional Content Of Crops, Caitlin R. Costello
The Effect Of Rhizophagus Intraradices Mycorrhizal Fungus On Iron Uptake Of Corn: A Global Overview On The Effects Of Climate Change On The Nutritional Content Of Crops, Caitlin R. Costello
PSU McNair Scholars Online Journal
Global iron deficiency is a major factor contributing to malnutrition and varied health conditions. One way to combat this is to maintain or increase the iron uptake of the globe’s major crops. Research has shown that certain types of mycorrhizal fungi can increase the nutrient uptake of plants. To further this research, we looked at the arbuscular fungi Rhizophagus intraradices and its potential relationship with the corn variety “Golden Bantam 8 Row”. This type of mycorrhizal fungi improves plant nutrition including the plant’s ability to source iron from the natural environment. Much of the iron found in the natural environment …
The Basic Communication Course Syllabus As A Rhetorical Document: The Impact Of Mediated Immediacy On Communication Apprehension With Instructors And Out-Of-Class Communication, Divine N. Aboagye, John Hooker, Cheri J. Simonds
The Basic Communication Course Syllabus As A Rhetorical Document: The Impact Of Mediated Immediacy On Communication Apprehension With Instructors And Out-Of-Class Communication, Divine N. Aboagye, John Hooker, Cheri J. Simonds
Basic Communication Course Annual
The purpose of this study was to examine teachers’ use of mediated immediacy in a syllabus to determine effects on students’ communication apprehension with instructors and student out-of-class communication with instructors. Participants viewed either a basic course syllabus with high levels of mediated immediacy or low levels of mediated immediacy and then completed surveys. The results showed that syllabi high in mediated immediacy made students significantly less apprehensive to communicate with instructors and more likely to engage in out-of-class communication with them. Implications for the use of mediated immediacy in syllabus construction are discussed.
Securing The Right Skills: A Longitudinal Assessment Of College Students’ Writing And Public Speaking Self-Efficacy, T. Kody Frey, Jessalyn I. Vallade
Securing The Right Skills: A Longitudinal Assessment Of College Students’ Writing And Public Speaking Self-Efficacy, T. Kody Frey, Jessalyn I. Vallade
Basic Communication Course Annual
This research investigated the developmental patterns of students’ writing and public-speaking self-efficacy throughout their experience in the basic communication course (BCC). Questions were posed regarding (a) whether students grew in their reported writing and public speaking self-efficacy over two semesters, (b) whether growth differed based on biological sex, and (c) whether affinity and apprehension (as sources of performance self-efficacy) played a role in student growth. Two multilevel models revealed significant differences in students’ initial status and rate of growth for each outcome. Specifically, sex, affinity, and apprehension influenced students’ starting positions in the course, while only apprehension had a significant …
The Ecology Of Human-Caused Mortality For A Protected Large Carnivore, John F. Benson, Kyle D. Dougherty, Paul Beier, Walter M. Boyce, Bogdan Cristescu, Daniel J. Gammons, David K. Garcelon, J. Mark Higley, Quinton E. Martins, Anna C. Nisi, Seth P. D. Riley, Jeff A. Sikich, Thomas R. Stephenson, T. Winston Vickers, Greta M. Wengert, Christopher C. Wilmers, Heiko U. Wittmer, Justin A. Dellinger
The Ecology Of Human-Caused Mortality For A Protected Large Carnivore, John F. Benson, Kyle D. Dougherty, Paul Beier, Walter M. Boyce, Bogdan Cristescu, Daniel J. Gammons, David K. Garcelon, J. Mark Higley, Quinton E. Martins, Anna C. Nisi, Seth P. D. Riley, Jeff A. Sikich, Thomas R. Stephenson, T. Winston Vickers, Greta M. Wengert, Christopher C. Wilmers, Heiko U. Wittmer, Justin A. Dellinger
School of Natural Resources: Faculty Publications
Mitigating human-caused mortality for large carnivores is a pressing global challenge for wildlife conservation. However, mortality is almost exclusively studied at local (within-population) scales creating a mismatch between our understanding of risk and the spatial extent most relevant to conservation and management of wide-ranging species. Here, we quantified mortality for 590 radio-collared mountain lions statewide across their distribution in California to identify drivers of human-caused mortality and investigate whether human-caused mortality is additive or compensatory. Human-caused mortality, primarily from conflict management and vehicles, exceeded natural mortality despite mountain lions being protected from hunting. Our data indicate that human-caused mortality is …
Mitigation Of Imidacloprid Toxicity In Poultry Chicken By Selenium Nanoparticles: Growth Performance, Lipid Peroxidation, And Blood Traits, Yahya Z. Eid, Yassin Omara, Asmaa Ragab, Ahmed Ismail
Mitigation Of Imidacloprid Toxicity In Poultry Chicken By Selenium Nanoparticles: Growth Performance, Lipid Peroxidation, And Blood Traits, Yahya Z. Eid, Yassin Omara, Asmaa Ragab, Ahmed Ismail
Faculty Journal Articles
No abstract provided.
Genesis Rabbah As Scripture: Testing The Bounds Of Theory, Text, And Event, Ryan Quandt
Genesis Rabbah As Scripture: Testing The Bounds Of Theory, Text, And Event, Ryan Quandt
Journal of Scriptural Reasoning
No abstract provided.
Atezolizumab-Induced Psoriasiform Drug Eruption Successfully Treated With Ixekizumab: A Case Report And Literature Review, Laura Gleason, E Hunter, Alexa J. Cohen, Jayson Suriano, Neda Nikbakht
Atezolizumab-Induced Psoriasiform Drug Eruption Successfully Treated With Ixekizumab: A Case Report And Literature Review, Laura Gleason, E Hunter, Alexa J. Cohen, Jayson Suriano, Neda Nikbakht
Department of Dermatology and Cutaneous Biology Faculty Papers
Immune-related cutaneous adverse events (ircAE) are commonly seen with immune checkpoint inhibitors such as atezolizumab. Atezolizumab-induced psoriasis has been previously reported as an ircAE, especially in patients with pre-existing psoriasis. The severity of the reaction influences treatment of the cutaneous eruption. Biologics should be considered as a treatment option for severe refractory psoriasiform eruptions even in patients with complex medical conditions like chronic infections and malignancy. This is the first reported case of successful treatment of atezolizumab-induced psoriasiform eruption with ixekizumab, a neutralizing IL17A monoclonal antibody, to the best of our knowledge. Herein, we present a 63-year-old man with a …
Defining Dementia-Friendly Communities From The Perspective Of Those Affected, Iris Alexandra Wernher
Defining Dementia-Friendly Communities From The Perspective Of Those Affected, Iris Alexandra Wernher
Dissertations and Theses
More and more communities across the globe are pledging to become more "dementia friendly," yet many initiatives lack direction as to what this pledge might entail. The intent of this qualitative study, conducted in the metropolitan area of Portland and several other cities in Oregon, was to better understand how communities can increase their dementia friendliness – from the perspective of people living with dementia and their care partners. The study further aimed to clarify if and how age- and dementia-friendly efforts can be integrated.
Twenty-five community-dwelling individuals living with dementia and their 25 informal carers participated separately in semi-structured …
Saving The Critically-Endangered Western Gray Whale, Jen Maresh
Saving The Critically-Endangered Western Gray Whale, Jen Maresh
Sustainability Research & Practice Seminar Presentations
Professor Jen Maresh, Biology - Saving the Western Gray Whale: How Science, Industry, and the Public are Coming Together to Protect a Critically Endangered Species from Oil & Gas Activities
"Blessed And Grateful … ": Longstanding Uab Employee Reflects On Life And Career, Micah Hardge
"Blessed And Grateful … ": Longstanding Uab Employee Reflects On Life And Career, Micah Hardge
Browse All News
No abstract provided.
Bison Influences On Composition And Diversity Of Riparian Plant Communities In Yellowstone National Park, J. Boone Kauffman, Dian Lyn Cummings, Cimarron Kauffman, Robert L. Beschta, Jeremy Brooks, Keeley Macneill, William J. Ripple
Bison Influences On Composition And Diversity Of Riparian Plant Communities In Yellowstone National Park, J. Boone Kauffman, Dian Lyn Cummings, Cimarron Kauffman, Robert L. Beschta, Jeremy Brooks, Keeley Macneill, William J. Ripple
Aspen Bibliography
Riparian zones are among the most biologically diverse ecosystems in the Intermountain West, USA, and provide valuable ecosystem services, including high rates of biotic productivity, nutrient processing, and carbon storage. Thus, their sustainability is a high priority for land managers. Large ungulates affect composition and structure of riparian/stream ecosystems through herbivory and physical effects, via trailing and trampling. Bison (Bison bison) in Yellowstone National Park (YNP) have been characterized as "ecosystem engineers" because of their demonstrated effects on phenology, aboveground productivity of grasses, and woody vegetation structure. Bison have greatly increased in numbers during the last two decades …
Covid-19 And Fall Rates In Older Adults: Longitudinal Study On The Impact Of Covid-19 On Geriatric Fall Rates In A Care Facility, Britton Holman
Covid-19 And Fall Rates In Older Adults: Longitudinal Study On The Impact Of Covid-19 On Geriatric Fall Rates In A Care Facility, Britton Holman
Doctor of Psychology (PsyD)
Falls in the geriatric population are continuing to increase around the world. Along with this, COVID-19 caused significant distress and changes in many older adult populations living in care facilities. Our study used archival data to determine if an association existed between fall rates at a specific care facility before COVID-19, during COVID-19, and during an outbreak of COVID-19 at the facility. An analysis of variance and Bonferroni’s post-hoc analysis was conducted, which found falls tended to be lower during outbreaks at the facility compared to pre-COVID-19 times. Reasons for this finding may be due to restrictions on activities and …
Bacterial Outer Membrane Vesicles Promote Lung Inflammatory Responses And Macrophage Activation Via Multi-Signaling Pathways, Sunhyo Ryu, Kareemah Ni, Chenghao Wang, Ayyanar Sivanantham, Johnathan M. Carnino, Hong-Long Ji, Yang Jin
Bacterial Outer Membrane Vesicles Promote Lung Inflammatory Responses And Macrophage Activation Via Multi-Signaling Pathways, Sunhyo Ryu, Kareemah Ni, Chenghao Wang, Ayyanar Sivanantham, Johnathan M. Carnino, Hong-Long Ji, Yang Jin
Cellular and Molecular Biology Faculty Publications and Presentations
Emerging evidence suggests that Gram-negative bacteria release bacterial outer membrane vesicles (OMVs) and that these play an important role in the pathogenesis of bacterial infectionmediated inflammatory responses and organ damage. Despite the fact that scattered reports have shown that OMVs released from Gram-negative bacteria may function via the TLR2/4-signaling pathway or induce pyroptosis in macrophages, our study reveals a more complex role of OMVs in the development of inflammatory lung responses and macrophage pro-inflammatory activation. We first confirmed that various types of Gram-negative bacteria release similar OMVs which prompt proinflammatory activation in both bone marrow-derived macrophages and lung alveolar macrophages. …
Bacterial Outer Membrane Vesicles Promote Lung Inflammatory Responses And Macrophage Activation Via Multi-Signaling Pathways, Sunhyo Ryu, Kareeman Ni, Chenghao Wang, Ayyanar Sivanantham, Jonathon M. Carnino, Hong-Long Ji, Yang Jin
Bacterial Outer Membrane Vesicles Promote Lung Inflammatory Responses And Macrophage Activation Via Multi-Signaling Pathways, Sunhyo Ryu, Kareeman Ni, Chenghao Wang, Ayyanar Sivanantham, Jonathon M. Carnino, Hong-Long Ji, Yang Jin
School of Medicine Faculty Publications and Presentations
Emerging evidence suggests that Gram-negative bacteria release bacterial outer membrane vesicles (OMVs) and that these play an important role in the pathogenesis of bacterial infection-mediated inflammatory responses and organ damage. Despite the fact that scattered reports have shown that OMVs released from Gram-negative bacteria may function via the TLR2/4-signaling pathway or induce pyroptosis in macrophages, our study reveals a more complex role of OMVs in the development of inflammatory lung responses and macrophage pro-inflammatory activation. We first confirmed that various types of Gram-negative bacteria release similar OMVs which prompt pro-inflammatory activation in both bone marrow-derived macrophages and lung alveolar macrophages. …
Intestinal Neuropod Cell Gucy2c Regulates Visceral Pain, Joshua R. Barton, Annie K. Londregran, Tyler D. Alexander, Ariana A. Entezari, Shely Bar-Ad, Lan Cheng, Angelo C. Lepore, Adam E. Snook, Manuel Covarrubias, Scott A. Waldman
Intestinal Neuropod Cell Gucy2c Regulates Visceral Pain, Joshua R. Barton, Annie K. Londregran, Tyler D. Alexander, Ariana A. Entezari, Shely Bar-Ad, Lan Cheng, Angelo C. Lepore, Adam E. Snook, Manuel Covarrubias, Scott A. Waldman
Department of Pharmacology and Experimental Therapeutics Faculty Papers
Visceral pain (VP) is a global problem with complex etiologies and limited therapeutic options. Guanylyl cyclase C (GUCY2C), an intestinal receptor producing cyclic GMP(cGMP), which regulates luminal fluid secretion, has emerged as a therapeutic target for VP. Indeed, FDA-approved GUCY2C agonists ameliorate VP in patients with chronic constipation syndromes, although analgesic mechanisms remain obscure. Here, we revealed that intestinal GUCY2C was selectively enriched in neuropod cells, a type of enteroendocrine cell that synapses with submucosal neurons in mice and humans. GUCY2Chi neuropod cells associated with cocultured dorsal root ganglia neurons and induced hyperexcitability, reducing the rheobase and increasing the resulting …
Form Cdc14 Phosphatase Contributes To Cell Wall Integrity And Pathogenesis In Candida Albicans, Kedric L. Milholland, Ahmed Abdelkhalek, Kortany M. Baker, Smriti Hoda, Andrew G. Demarco, Noelle H. Naughton, Angela N. Koeberlein, Gabrielle R. Lorenz, Kartikan Anandasothy, Antonio Esperilla-Muñoz, Sanjeev K K. Narayanan, Jaime Correa-Bordes, Scott D. Briggs, Mark C. Hall
Form Cdc14 Phosphatase Contributes To Cell Wall Integrity And Pathogenesis In Candida Albicans, Kedric L. Milholland, Ahmed Abdelkhalek, Kortany M. Baker, Smriti Hoda, Andrew G. Demarco, Noelle H. Naughton, Angela N. Koeberlein, Gabrielle R. Lorenz, Kartikan Anandasothy, Antonio Esperilla-Muñoz, Sanjeev K K. Narayanan, Jaime Correa-Bordes, Scott D. Briggs, Mark C. Hall
Purdue University Libraries Open Access Publishing Fund
The Cdc14 phosphatase family is highly conserved in fungi. In Saccharomyces cerevisiae, Cdc14 is essential for down-regulation of cyclin-dependent kinase activity at mitotic exit. However, this essential function is not broadly conserved and requires only a small fraction of normal Cdc14 activity. Here, we identified an invariant motif in the disordered C-terminal tail of fungal Cdc14 enzymes that is required for full enzyme activity. Mutation of this motif reduced Cdc14 catalytic rate and provided a tool for studying the biological significance of high Cdc14 activity. A S. cerevisiae strain expressing the reduced-activity hypomorphic mutant allele (cdc14hm ) as the …