Digital Commons Network^™

Process Automation And Robotics Engineering For Industrial Processing Systems, Drake Stimpson

USF Tampa Graduate Theses and Dissertations

Automation in industrial systems applications has emerged as the fundamental solution for improving quality, production rate, and efficiency of a process. Much of the recent popularity surrounding the transition of processes from manually operated tasks to automated systems can be attributed to the concept of Industry 4.0, which outlines the fundamental guidelines for integrating cyber-physical systems into industrial processes. Due to rapid advancement of technology in robotics and automation as well as the increase in accessibility of resources to this technology, the capability to develop automated systems has become feasible for small-scale enterprise. This work presents a two-part initiative to …

Using Game Theory To Model Tripolar Deterrence And Escalation Dynamics, Grace Farson

Honors Program: Senior Projects (Public)

The study investigated how game theory can been utilized to model multipolar escalation dynamics between Russia, China, and the United States. In addition, the study focused on analyzing various parameters that affected potential conflict outcomes to further new deterrence thought in a tripolar environment.

A preliminary game theoretic model was created to model and analyze escalation dynamics. The model was built upon framework presented by Zagare and Kilgour in their work ‘Perfect Deterrence’. The model is based on assumptions and rules set prior to game play. The model was then analyzed based upon these assumptions using a form of mathematical …

Relationships Between Burnout, Isolation, Secondary Trauma, And Self-Compassion In Mental Health Providers, Michael Underriner

Doctor of Psychology (PsyD)

Experiencing burnout as a mental health provider is a growing concern with a number of personal and professional issues related to it; organizational and self-directed interventions are often used to combat the issue (Morse et al., 2012). With the onset of coronavirus (COVID-19), mental health providers face more isolation at work due to social distancing measures and more instances of hearing trauma reports from their patients. Self-compassion might be a solution to address the concerns stated above (McCade et al., 2021; Richardson et al, 2018). Participants in this study were members of a state psychological association, many of whom are …

Interactions Of Snps In Folate Metabolism Related Genes On Prostate Cancer Aggressiveness In European Americans And African Americans, Hui Yi Lin, Susan E. Steck, Indrani Sarkar, Elizabeth T.H. Fontham, Alan Diekman, Lora J. Rogers, Calvin T. Ratliff, Jeannette T. Bensen, James L. Mohler, L. Joseph Su

School of Public Health Faculty Publications

Background: Studies showed that folate and related single nucleotide polymorphisms (SNPs) could predict prostate cancer (PCa) risk. However, little is known about the interactions of folate-related SNPs associated with PCa aggressiveness. The study’s objective is to evaluate SNP–SNP interactions among the DHFR 19-bp polymorphism and 10 SNPs in folate metabolism and the one-carbon metabolism pathway associated with PCa aggressiveness. Methods: We evaluated 1294 PCa patients, including 690 European Americans (EAs) and 604 African Americans (AAs). Both individual SNP effects and pairwise SNP–SNP interactions were analyzed. Results: None of the 11 individual polymorphisms were significant for EAs and AAs. Three SNP–SNP …

Sources Of Atmospheric Dust Deposition On Utah Lake, Justin Telfer

Theses and Dissertations

Atmospheric deposition (AD) is a significant source of nutrient loading to waterbodies around the world. However, the sources and loading rates are poorly understood for major waterbodies and even less understood for local waterbodies. Utah Lake is a eutrophic lake located in central Utah, USA, and has high nutrient levels. Recent research has identified AD as significant sources for nutrient loading to the lake to better understand the dust AD sources, we sampled suspected source locations and collected deposition samples around the lake. We analyzed these samples using Inductively Coupled Plasma (ICP) for 25 metals to characterize their elemental fingerprints. …

Towards Meaningful Research And Engagement: Indigenous Knowledge Systems And Great Lakes Governance, Deborah Mcgregor, Nicole Latulippe, Rod Whitlow, Kristi Leora Gansworth, Lorrilee Mcgregor, Stephanie Allen

Articles & Book Chapters

For thousands of years, Indigenous peoples governed their relations in the Great Lakes region, guided by distinct political, legal, governance, and knowledge systems. Despite historic and ongoing exclusion of Indigenous peoples from Great Lakes governance in the Canadian context and other assaults on Indigenous sovereignty, authority, jurisdiction and responsibilities, Indigenous peoples have maintained their relationships with the Great Lakes. In recent years, Indigenous knowledge systems (IKS) have made inroads in Great Lakes governance, thanks primarily to First Nation political advocacy. However, it remains a challenge to include Indigenous knowledge and implement approaches that bridge Indigenous and Western ways of knowing. …

Induction Of Antimicrobial Protein S100a15 Expression By Oral Microbial Pathogens Is Toll-Like Receptors-Dependent Activation Of C-Jun-N-Terminal Kinase (Jnk), P38, And Nf-Κb Pathways, Denis Selimovic, Naji Kharouf, Florence Carrouel, Sofie Yasmin Hassan, Thomas W. Flanagan, Sarah Lilly Hassan, Mosaad Megahed, Youssef Haikel, Simeon Santourlidis, Mohamed Hassan

School of Medicine Faculty Publications

The antimicrobial protein S100A15 belongs to the S100 family, which is differentially expressed in a variety of normal and pathological tissues. Although the function of S100A15 protein has been discussed in several studies, its induction and regulation in oral mucosa, so far, are largely unknown. In this study, we demonstrate that S100A15 is induced by the stimulation of oral mucosa with gram− or gram+ bacterial pathogens, as well as with the purified membrane components, namely lipopolysaccharides (LPS) and lipoteichoic acid (LTA). The stimulation of the human gingival fibroblast (GF) and the human mouth epidermal carcinoma (KB) cell lines with either …

Biotechnology Lab Manual Course Bi-453, Sarbani Ghoshal, Nidhi Gadura

Open Educational Resources

The purpose of the lab manual is to acquaint undergraduate students with hands-on training on key laboratory experiments needed to gain expertise in Biotechnology, Cell & Molecular Biology.

Numerical Study And Knn Method Application In Choosing Biomaterials Dedicated To Dental Implantology, Amel Soualhi, Mourad Brioua, Rachid Benbouta

Emirates Journal for Engineering Research

The dental implant is the equivalent of a tooth root well anchored in the jaw. This research paper aimed to study the characteristics of biomaterials used for dental implants using numerical methods in order to select which has better properties and is closer to the bone. The pattern of stress distribution in the jaw and implant can result in bone restoration and implant failure. In this context, a three-dimensional finite element (3D) model of implant/bone system has been established, for two materials, to predict the stress distribution and further studies the influence of length, threading and coating, in order to …

Cell Volume As A Determinant Of Virus-Mediated Population Growth In Ciliates, Jace Miller, John Paul Delong Phd

Honors Program: Senior Projects (Public)

Many protists and other small aquatic organisms consume virus particles, a behavior known as virovory. Some species of protists, such as the ciliate Halteria grandinella, can grow and divide using viruses as their sole food source. Other ciliate species have previously been shown to consume large quantities of viral particles, but it is unclear if they are able to support population growth with viruses alone. Because large ciliates have a higher energy demand, we hypothesize that they will be unable to support population growth on a virus-only diet. We fed nine ciliate species a diet of chloroviruses and found …

Role Of The Cytosolic Chaperonin Cct In The Folding Of Novel Substrates, Theresa M. Smith

Theses and Dissertations

All cells depend on properly folded proteins for survival and function. Misfolding of proteins results in loss of critical functions and may trigger the misfolding of other nearby proteins leading to toxic aggregation. While many proteins can fold on their own, others with complicated domain structures require assistance from protein folding machines called chaperones. The most complex and highly specialized of all chaperones is the eukaryotic chaperonin complex CCT which is necessary for the folding of a wide variety of essential proteins. These include the cytoskeletal proteins actin and tubulin as well as the Gβ subunit of the G protein …

Dignity And Respect At All Times: How Federal Agencies Can Measure Up In Complying With Nagpra And Related Statutes, Catherine E. Kanatas, Maxwell Smith

Duke Environmental Law & Policy Forum

No abstract provided.

The Fashion Industry Is Not As "Green" As It Would Like You To Believe, Ashly Riches

Duke Environmental Law & Policy Forum

No abstract provided.

Association Of Excessive Precipitation And Agricultural Land Use With Honey Bee Colony Performance, Gabriela M. Quinlan, Rufus Isaacs, Clint R. V. Otto, Autumn H. Smart, Meghan O. Milbrath

Department of Entomology: Faculty Publications

Context From landscape variables to weather, multiple environmental factors affect honey bees and other pollinators. Detailed honey bee colony assessments in a variety of landscape and weather conditions offer the opportunity to develop a mechanistic understanding of how landscape composition, configuration, and weather are associated with colony nutrition, demography, and productivity.

Objectives Our objective was to test if weather and landscape characteristics (e.g., agricultural versus forested land use) are associated with different honey bee colony outcomes (foraged nectar mass, foraged pollen mass, pupal population size, and adult population size change).

Methods We collected detailed colony measurements on over 450 honey …

Progranulin Oncogenic Network In Solid Tumors, Elisa Ventura, Giacomo Ducci, Reyes Benot Dominguez, Valentina Ruggiero, Antonino Belfiore, Elena Sacco, Marco Vanoni, Renato V. Iozzo, Antonio Giordano, Andrea Morrione

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Progranulin is a pleiotropic growth factor with important physiological roles in embryogenesis and maintenance of adult tissue homeostasis. While-progranulin deficiency is associated with a broad range of pathological conditions affecting the brain, such as frontotemporal dementia and neuronal ceroid lipofuscinosis, progranulin upregulation characterizes many tumors, including brain tumors, multiple myeloma, leiomyosarcoma, mesothelioma and epithelial cancers such as ovarian, liver, breast, bladder, adrenal, prostate and kidney carcinomas. The increase of progranulin levels in tumors might have diagnostic and prognostic significance. In cancer, progranulin has a pro-tumorigenic role by promoting cancer cell proliferation, migration, invasiveness, anchorage-independent growth and resistance to chemotherapy. In …

Addressing Barriers To Proactive Restoration Of At-Risk Sagebrush Communities: A Causal Layered Analysis, Carmen Calzado-Martinez, Mark W. Brunson, Sofia Koutzoukis, Jacobo Baggio, Kari E. Veblen

Environment and Society Faculty Publications

Restoration success in degraded rangelands often depends on a site's resilience to disturbance and resistance to invasive plants. Because it is more difficult to restore plant communities after they are dominated by invasive species, a potential approach is proactive restoration in sites at risk of crossing degradation thresholds (e.g. initiating restoration prior to invasive grass dominance). When developing a new restoration approach, it is important to consider operational feasibility, including social, budgetary, and environmental factors. Accordingly, we studied influences within land management agencies on the adoption of a specific proactive restoration approach: out-planting native grass and forb seedlings into sagebrush …

Inhibition Of Src-3 As A Potential Therapeutic Strategy For Aggressive Mantle Cell Lymphoma, Jianwei Chen, Shelby Sloan, Lapo Alinari, David M Lonard, Bert W O'Malley, Michael Wang, Jin Wang

Faculty, Staff and Students Publications

Mantle cell lymphoma (MCL) is a heterogeneous disease with a poor prognosis. Despite years of research in MCL, relapse occurs in patients with current therapeutic options necessitating the development of novel therapeutic agents. Previous attempts to pharmacologically inhibit SRC-3 show effectiveness in vivo and in vitro in other B cell lymphomas, and previous studies have shown that SRC-3 is highly expressed in the lymph nodes of B cell non-Hodgkin’s lymphoma patients. This suggests that SRC-3 may play a role in the progression of B cell lymphoma and that the development of selective SRC inhibitors should be investigated. This study aimed …

Thermodynamic Effects Drive Countergradient Responses In The Thermal Performance Of Littorina Saxatilis Across Latitude, Christopher Dwane, Enrico L. Rezende, Oliver Tills, Juan Galindo, Emilio Rolán-Alvarez, Simon Rundle, Manuela Truebano

School of Biological and Marine Sciences

Thermal performance curves (TPCs) provide a powerful framework to assess the evolution of thermal sensitivity in populations exposed to divergent selection regimes across latitude. However, there is a lack of consensus regarding the extent to which physiological adjustments that compensate for latitudinal temperature variation (metabolic cold adaptation; MCA) may alter the shape of TPCs, including potential repercussion on upper thermal limits. To address this, we compared TPCs for cardiac activity in latitudinally-separated populations of the intertidal periwinkle Littorina saxatilis. We applied a non-linear TPC modelling approach to explore how different metrics governing the shape of TPCs varied systematically in response …

Mechanism Of Kmt5b Haploinsufficiency In Neurodevelopment In Humans And Mice, Sarah E Sheppard, Marisa Andrews, Dustin Baldridge, John Constantino, Et Al.

2020-Current year OA Pubs

Pathogenic variants in

How Are Secure Attachment Relationships Fostered Through Talk Between Teachers And Students Who Have Been Adopted? A Conversation Analysis, R Phillips, R Dallos, S Minton, P Keohane

School of Psychology

Despite the growing recognition of the importance of supportive teacher–student relationships to create safety for young people who have experienced early adversity and trauma, there is not a clear understanding of what factors make for positive school relationships and how these can be fostered. The aims of the study were to explore how children with challenging emotional backgrounds are supported by their key adult in school and how this occurs in the process of conversations between them. Three student–teacher pairs from a specialist school took part in a semi-structured interview about their relationship. Data was analysed using conversation analysis. Analysis …

Epidermal Threads Reveal The Origin Of Hagfish Slime, Yu Zeng, David C. Plachetzki, Kristen Nieders, Hannah Campbell, Marissa Cartee, M. Sabrina Pankey, Kennedy Guillen, Douglas Fudge

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

When attacked, hagfishes produce a soft, fibrous defensive slime within a fraction of a second by ejecting mucus and threads into seawater. The rapid setup and remarkable expansion of the slime make it a highly effective and unique form of defense. How this biomaterial evolved is unknown, although circumstantial evidence points to the epidermis as the origin of the thread- and mucus-producing cells in the slime glands. Here, we describe large intracellular threads within a putatively homologous cell type from hagfish epidermis. These epidermal threads averaged ~2 mm in length and ~0.5 μm in diameter. The entire hagfish body is …

Long-Term Morbidity And Mortality Among Survivors Of Neuroblastoma Diagnosed During Infancy: A Report From The Childhood Cancer Survivor Study, Danielle Novetsky Friedman, Pamela J Goodman, Wendy M Leisenring, Lisa R Diller, Susan L Cohn, Susan A Smith, Suzanne L Wolden, Joseph P Neglia, Kirsten K Ness, Todd M Gibson, Paul C Nathan, Brent R Weil, Leslie L Robison, Kevin C Oeffinger, Gregory T Armstrong, Charles A Sklar, Tara O Henderson

Faculty, Staff and Student Publications

Purpose: To describe the risk of late mortality, subsequent malignant neoplasms (SMNs), and chronic health conditions (CHCs) in survivors of neuroblastoma diagnosed in infancy by treatment era and exposures.

Methods: Among 5-year survivors of neuroblastoma in the Childhood Cancer Survivor Study diagnosed age < 1 year between 1970 and 1999, we examined the cumulative incidence of late (> 5 years from diagnosis) mortality, SMN, and CHCs (grades 2-5 and 3-5). Multivariable Cox regression models estimated hazard ratios (HRs) and 95% CIs by decade and treatment (surgery-alone v chemotherapy with or without surgery [C ± S] v radiation with or without chemotherapy ± surgery [R ± C ± S]) among survivors and between survivors and 5,051 …

Towards A Biomarker For Acute Arterial Thrombosis Using Complete Blood Count And White Blood Cell Differential Parameters In Mice, Hee Jeong Jang, Dawid Schellingerhout, Jiwon Kim, Jinyong Chung, Dong-Eog Kim

Faculty, Staff and Student Publications

There is no blood biomarker diagnostic of arterial thrombosis. We investigated if arterial thrombosis per se was associated with alterations in complete blood count (CBC) and white blood cell (WBC) differential count in mice. Twelve-week-old C57Bl/6 mice were used for FeCl3-mediated carotid thrombosis (n = 72), sham-operation (n = 79), or non-operation (n = 26). Monocyte count (/µL) at 30-min after thrombosis (median 160 [interquartile range 140-280]) was ~ 1.3-fold higher than at 30-min after sham-operation (120 [77.5-170]), and twofold higher than in non-operated mice (80 [47.5-92.5]). At day-1 and -4 post-thrombosis, compared with 30-min, monocyte count decreased by about …

Structure-Based Regulatory Role For The 5'Utr Of Rcnmv Rna2, Jennifer S H Im, Jasmine R Sheppard, K Andrew White

Faculty, Staff and Student Publications

Red clover necrotic mosaic virus (RCNMV) is a segmented positive-strand RNA virus consisting of RNA1 and RNA2. Previous studies demonstrated that efficient translation of RCNMV RNA2 requires de novo synthesis of RNA2 during infections, suggesting that RNA2 replication is required for its translation. We explored a potential mechanism underlying the regulation of replication-associated translation of RNA2 by examining RNA elements in its 5' untranslated region (5'UTR). Structural analysis of the 5'UTR suggested that it can form two mutually exclusive configurations: a more thermodynamically stable conformation, termed the 5'-basal stem structure (5'BS), in which 5'-terminal sequences are base paired, and an …

Differential Spatial Gene And Protein Expression Associated With Recurrence Following Chemoradiation For Localized Anal Squamous Cell Cancer, Sharia Hernandez, Prajnan Das, Emma B Holliday, Li Shen, Wei Lu, Craig A Messick, John Skibber, Ethan B Ludmir, Y Nancy You, Grace Li Smith, Brian Bednarski, Larisa Kostousov, Eugene J Koay, Bruce D Minsky, Matthew Tillman, Shaelynn Portier, Cathy Eng, Albert C Koong, George J Chang, Wai Chin Foo, Jing Wang, Luisa Solis Soto, Van K Morris

Faculty, Staff and Student Publications

The identification of transcriptomic and protein biomarkers prognosticating recurrence risk after chemoradiation of localized squamous cell carcinoma of the anus (SCCA) has been limited by a lack of available fresh tissue at initial presentation. We analyzed archival FFPE SCCA specimens from pretreatment biopsies prior to chemoradiation for protein and RNA biomarkers from patients with localized SCCA who recurred (N = 23) and who did not recur (N = 25). Tumor cells and the tumor microenvironment (TME) were analyzed separately to identify biomarkers with significantly different expression between the recurrent and non-recurrent groups. Recurrent patients had higher mean protein expression of …

Brain Injury Accelerates The Onset Of A Reversible Age-Related Microglial Phenotype Associated With Inflammatory Neurodegeneration, Sarah J Doran, Romeesa Khan, Chunfeng Tan, Shaolin Liu, Bogdan A Stoica, Alan I Faden, Gregory Szeto, David J Loane, Junfang Wu

Faculty, Staff and Student Publications

Lipofuscin is an autofluorescent (AF) pigment formed by lipids and misfolded proteins, which accumulates in postmitotic cells with advanced age. Here, we immunophenotyped microglia in the brain of old C57BL/6 mice (>18 months old) and demonstrate that in comparison to young mice, one-third of old microglia are AF, characterized by profound changes in lipid and iron content, phagocytic activity, and oxidative stress. Pharmacological depletion of microglia in old mice eliminated the AF microglia following repopulation and reversed microglial dysfunction. Age-related neurological deficits and neurodegeneration after traumatic brain injury (TBI) were attenuated in old mice lacking AF microglia. Furthermore, increased …

Applying Contemplative Practices To The Educational Design Of Mathematics Content: Report From A Pioneering Workshop, Patricia Morgan, Dor Abrahamson

Journal of Contemplative Inquiry

Researchers in the field of mathematics education are beginning to appreciate the potential of contemplative practices such as mindfulness to alleviate students’ stress and increase their focus. What researchers do not yet know is whether, and if so how, bringing focused attention to somatic experience through a wide variety of contemplative–somatic practices (i.e., yoga, Feldenkrais, body–mind centering, and attending to bodily sensations in meditation) may support student learning of specific mathematical content. As a first step toward conceptualizing and ideating the pedagogical design and facilitation of content-oriented contemplative exercises, we convened a workshop to explore these ideas. Here we report …

Classical Physics And Human Embodiment: The Role Of Contemplative Practice In Integrating Formal Theory And Personal Experience In The Undergraduate Physics Curriculum, Zosia Krusberg, Meredith Ward

Journal of Contemplative Inquiry

One of the objectives of the undergraduate physics curriculum is for students to become aware of the connections between the fundamental principles of classical physics and their personal experience. Nonetheless, numerous studies have shown that students’ awareness of such connections tends to deteriorate, sometimes substantially, following instruction. In this work, which constitutes the first analysis of the effects of contemplative practices on the learning experience in undergraduate physics courses, we present two practices aimed at integrating formal theory with students’ personal, embodied experience: a sensory meditation and a contemplative videography. In written reflections on their experiences with the practices, the …

Many Rivers, One Ocean: An Initiative For Contemplative Study And Practice, Deborah Kipps-Vaughan, Jared Featherstone, Edward Brantmeier, Marsha Mays-Bernard, Kimberly Duvall, Shari Scofield

Journal of Contemplative Inquiry

Contemplative practices, with their intentions toward well-being, authentic relationships, and deep learning, are beneficial to a community. Therefore, a sense of responsibility exists for universities to explore avenues for developing a culture of contemplative inquiry. This article describes a process embarked upon by members of a large state university community in their intentional pursuit to develop a more pervasive contemplative community on campus. Several faculty and staff with well-established personal practices in mind-body exercise, meditation, and mindfulness were motivated, within their respective professional roles, to incorporate contemplative work in classrooms and programs. Such integration is increasingly common at universities of …

Soulfulness As An Orientation To Contemplative Practice: Culture, Liberation, And Mindful Awareness, Shelly P. Harrell

Journal of Contemplative Inquiry

Soulfulness is introduced here as an orientation to contemplative practice that centers a synergistic integration of the psychological, spiritual, and cultural dimensions of soul (deepness, aliveness, authenticity, and a healing/transformative resource) to inform the design and implementation of culturally attuned methods. Soulfulness is characterized by themes emerging from diasporic African cultural influences and inspired by an African American cultural sensibility. These themes include an ethos of interconnectedness, a relational/communal sensibility, the centrality of spirituality, creativity and improvisation, a holistic orientation to human experience, emotional expressiveness, resilience and overcoming adversity, and struggles for liberation in the context of historical and ongoing …